Emil Ludwig, Ernest Newman, H. Shachter, J. T. C. Moore-Brabazon, Major the Hon. J. C. Darling, S. J. Young, O. M. Green, E. N., John Hodgkin, L. St. Clare Grondona (Author of "Empire Stock-taking"), H. E. Dale, Philip Kent, Ralph Straus, Mary MacLeod Moore, Sir Sidney Low, Sydney Carroll, Herbert Sidebotham, Yarborogh, Arthur Keith, William C. Hall, J. M. Bulloch, S. W., Gerard F. K. Heller, A. Loscombe Wallis, Walter Dexter, E. V. Lucas, Carew Mildmay, Laurie Magnus, H. W. B., James Agate, Frank Rutter, E. H. Price, H. H. Hammond, George C. Stead, D. R. Gent, C. F. Annesley Voysey, F. French, Desmond MacCarthy, Atticus, H. C. Minchin, W. B. Winckworth, M. Pollock Smith, J. T. S. Stopford, F. D. D'evelyn, Nesta Hunter, Sir Philip Gibbs, Clara Watts-Dunton, L. Van Vliet, P. W. Johnston, T. W. Tyrrell, Dilys Powell, H. R. Barbor, J. M. B., Rowland H. Hill, Louis Roche, T. Earle Welby, H. F., Hamilton Price, D. P., Charles Lowe, Jean N. Norris, Frank Bonnett, John A. Barton, R. J. Barrett Financial Editor, F. J. O'hare,
... By P. C. Wren. (Murray. 7s. 6d) "The Minion." By Rafael Sabatini. (Hutchinson. 7s. 6d) "Lamplight." By ... The Lovers of Astrea The Whistling Thorn The Minion The Guillone Hutchinson Multiple Display Advertising Items Frank ...
1930 - Gale Group | Sunday Times HA GDA
Whitney Whitford, Victoria Hawkins, Kriebashne S. Moodley, Matthew J. Grant, Klaus Lehnert, Russell G. Snell, Jessie C. Jacobsen,
... throughput laboratories. The application of Oxford Nanopore Technologies’ MinION sequencer has the potential to fulfil this requirement. ... a multiplexing assay that pools PCR amplicons for MinION sequencing to enable sequencing of multiple templates from ... and sample pooling was developed for simultaneous multiplex MinION sequencing of 100 PCR amplicons. The amplicons are ... affected individuals following Illumina exome sequencing. The pooled MinION sequences were deconvoluted by aligning to custom references ...
Tópico(s): CRISPR and Genetic Engineering
2022 - Nature Portfolio | Scientific Reports
By Hugh Noyes, Parliamentary Correspondent, By David Felton, Labour Reporter, By David Hewson, From Roger Boyes, From Nicholas Ashford, By Julian Haviland Political Editor, By Paul Routledge, Labour Editor, By Diana Geddes, Education Correspondent, By Philip Webster, Political Reporter, By Frances Gibb, By Nicholas Timmins, By Lucy Hodges, By the Staff of "Nature", By Tony Samstag, By David Walker, From Richard Ford, By Anthony Bevins Political Correspondent, By Hugh Clayton Environment Correspondent, From Our Correspondent, From Trevor Fishlock, From Michael Hamlyn, From David Bonavia, From Ian Murray, From Godfrey Morrison, From Ray Kennedy, John Earle, From Edward Mortimer, From Olli Kivinen, From Peter David, By David Cross, From Michael Binyon, Moscow, Jan 19, By staff reporters in London, Trevor Fishlock, Dennis Hackett, John Russell Taylor, Richard Williams, Irving Wardle, John Percival, John Higgins, Paul Griffiths, Noël Goodwin, by Christopher Johnson, Henry Fairlie, Peter Watson, by Michael Meacher, STEPHEN HASTINGS, GLADWYN, HUGH JENKINS, JOHN GIELGUD, LOUIS KENTNER, ANDREW MARTIN, PETER FRANKL, KATALIN SCHPFLIN, GEORGE MIKES, ANDRE DEUTSCH, GABRIEL RONAY, VICTOR G. AMBRUS, ANTHONY WIGRAM, ANDREW GILCHRIST, PETER WOOD, B. M. WRIGHT, PAUL THEROUX, MICHAEL KITSON, JANE BROWN, MICHAEL MANSER, EILEEN LORAM, By Kenneth Gosling, By Alan Hamilton, A correspondent, Michael Clark, By Philip Robinson, Paul Maidment, By Frances Williams, By Margareta Pagano, By Peter Wilson-Smith, From Bailey Morris, By Edward Townsend Industrial Correspondent, Sally White, From Michael Parrott, Adrienne Gleeson, From John Woodcock Cricket Correspondent, By John Hennessy, By Keith Macklin, By Leslie Duxbury, By Nicholas Harling, By Stuart Jones Football Correspondent, By Paul Newman, Derek Wyatt, By Norman Fox Athletics Correspondent, By Richard Eaton, By Peter West Rugby Correspondent, By Sydney Friskin, By John Karter, By Gordon Allan, John Blunsden, by Baron Phillips, Edited by Peter Dear, From George Clark European Political Corr., From Charles Hargrove,
... Important Notic To Travellers, The Spastics Society, Rover, Minion, Bl, Secretaries Plus, Stannah Lifts, Graduate Girls Secretarial, ...
1982 - Gale Group | TDA
Amrita Srivathsan, Leshon Lee, Kazutaka Katoh, Emily Hartop, Sujatha Narayanan Kutty, Johnathan Wong, Darren Yeo, Rudolf Meier,
... from specimen to sequence by using the portable MinION sequencer.We describe how tagged amplicons can be obtained and sequenced with the real-time MinION sequencer in many settings (field stations, biodiversity labs, ... on several runs of the latest generation of MinION flow cells ("R10.3") which suggest that each ... ONTbarcoder, which overcomes the bioinformatics challenges posed by MinION reads. The software is compatible with Windows 10, ... input files (FASTQ, demultiplexing file). We document that MinION barcodes are virtually identical to Sanger and Illumina ...
Tópico(s): Identification and Quantification in Food
2021 - BioMed Central | BMC Biology
From Clifford Webb, Midlands Industrial Correspondent, From Michael Binyon, , By Our Political Correspondent, By David Felton Labour Reporter, By Julian Haviland, Political Editor, From Charles Hargrove, , By Anthony Bevins Political Correspondent, By Christopher Warman Arts Correspondent, By Anthony Bevins, Political Correspondent, By Paul Routledge, Labour Editor, By Ian Bradley, By David Felton, Labour Reporter, By Annabel Ferriman Health Services Correspondent, By Diana Geddes Education Correspondent, By the Staff of "Nature", By Pearce Wright, Science Editor, From Arthur Osman Birmingham, By John Young, Agriculture Correspondent, From Our Correspondent, , By David Nicholson, , By Diana Geddes, Education Correspondent, By a Staff Reporter, By David Walker, By Lucy Hodges, By Peter Henuessy, From Our Correspondent York, By Frances Gibb, From Henry Stanhope, Defence Correspondent, , From W. P. Reeves, , From Patricia Clough, From Peter Hazelhurst, , From Richard Wigg, , By Caroline Moorehead, From Nicholas Hirst, From Ivor Davis, From Robert Fisk, , From Christopher Walker, , From Our Correspondent, From Trevor Fishlock, From Moshe Brilliant, From Frederick Bonnart, From Roger Boyes, , From Peter Norman, From Dessa Trevisan, , By Denis Taylor, From David Bonavia, , DES WHSON, by Susan Beattie, by Robert Fisk, Peter Watson, by Eric Heffer, JOHN HUNT., S. ZUCKERMAN., DAVID LIDDELL-KING, , DENIS O'BRIEN., T. MERVYN JONES., A. W. STANFORTH, , KONRAD SYROP., HARRY HODGKINSON, , T. M. McCARTHY., STUART HAMPSHIRE, , BRUCE ELSMORE, , M. B. DANIELS, , ESMOND WARNER, , By a Bridge Correspondent, By Geraldine Norman, Sale Room Correspondent, From Our Own Correspondent, Miles Kington, By Harry Golombek Chess Correspondent, Dennis Hackett, John Russell Taylor, Stanley Sadie, William Mann, Noel Goodwin, John Higgins, By Adriènne Gleeson, By Hugh Noyes Parliamentary Correspondent, By Ronald Pullen, By Peter Wilson-Smith Banking Correspondent, By Melvyn Westlake, By Peter Hill, Industrial Editor, From Bailey Morris, , By Elkan Allan, By Michael Prest, By Clive Cookson and Michael Parrott, By Peter Hill Industrial Editor, By Our Financial Staff, By Derek Harris, Commercial Editor, By Our Industrial Staff, By Ronald Kershaw, Northern Industrial Correspondent, BY THE FINANCIAL EDITOR, Ross Davies, By Paul Maidment, By Peter West Rugby Correspondent, By Peter Aykroyd, By Norman Fox, By Robert Pryce, From Barry Pickthall, By Roy McKelvie, From Richard Streeton, By Michael Seely, By Nicholas Harling, Edited by Peter Dear, By Kenneth Gosling,
... Display Advertising: The Royal Ballet, Bank Lending Rates, Minion, Garrard, Tate Gallery, Unilever N.V., Rediffusion, Nch, ...
1981 - Gale Group | TDA
Benedict M. Matern, Timo I. Olieslagers, Mathijs Groeneweg, Burcu Duygu, Lotte Wieten, Marcel G.J. Tilanus, Christina E.M. Voorter,
... stem cell transplantation. Single-molecule sequencing (PacBio or MinION) is a newest (third) generation sequencing approach. MinION is a nanopore sequencing platform, which provides long ... used to compare third field sequences obtained by MinION, with Sanger sequence–based typing showing a 100% ... set the acceptance criteria for the use of MinION in a routine setting. The acceptance criteria were ... stem cell transplantation. Single-molecule sequencing (PacBio or MinION) is a newest (third) generation sequencing approach. MinION ...
Tópico(s): Immune Cell Function and Interaction
2020 - Elsevier BV | Journal of Molecular Diagnostics
Andrew Robson, Dr Thomas Stuttaford Medical Briefing, Ian Whitlock, Rosemary Righter, Matthew Syed, Andrew Pierce, DJM, Neelam Verjee, Adam Sherwin Media Reporter, Sarah Potter, Frances Gibb Legal Editor, Geoff Prescott (Chief Executive), Magnus Linklater, Peter Lansley, Adam Isaacs, David Rhys Jones, Richard Hobson, Ivo Tennant, Zahid Hussein, Sylvia Lachmann, Philip Howard, Jennal Cox Fitness Editor, David Chater, Suna Erdem, P. B. Knowles, Nic Hopkins, Colin Bullen, Sheila Johnston, Barbara Ellen, Robert Black, Patrick Barkham, Kevin Eason, Jon Valur Jensson, Joe Joseph, Antonia Senior, Ben Webster Transport Correspondent, Peter Wyman, Tina Brown, Stewart Tendler Crime Correspondent, Dominic Walsh, James Doran Wall Street Correspondent, Debra Craine, Rory Watson, Gary Jacob, Joe Morgan, David Castree, Daniel Finkelstein, Robert Thomson and Leo Lewis, Dan Sabbagh, Roland Watson and Richard Owen, Phoebe Greenwood, Robin Young, Alan Hamilton, Stephen Farrell, Oliver Wright Health Correspondent, David Eimer, Nick Wyke, Anatole Kaletsky, Michael Bechley, Valerie Elliott Consumer Editor, Roland Watson, Dan Stubbs, David Hands, Matt Dickinson Chief Football Correspondent, Lisa Armstrong Fashion Editor, David Charter, Nicholas Wapshott, Patience Wheatcroft Business Editor, Mohammed Al-Hilli, Mary Ring, Tony Halpin Education Editor, Daniel McGrory, Suzy Jagger, Oliver Kay, Christine Selb, Charles Bremner, Philip Webster, Roland Watson and James Bone, Rick Broadbent, Tom Baldwin, Jill Sherman Whitehall Editor, Mark Henderson Science Correspondent, Christopher Martin-Jenkins, Rob Wright, Elaine Monaghan, Stephen Farrell and Robert Talt and Richard Beeston, Neil Harman Tennis Correspondent, Raymond Keene, Lewis Smith, Sean MacAulay, Philip Whiteley, M. Spensley, David Martin, Christine Buckley Industrial Editor, Beryl Dixon, Greg Hurst Parliamentary Correspondent, Chris Campling, Russell Hotten, Richard Owen, Christopher Martin-Jenkins Chief Cricket Correspondent, Clem Cecil, Oliver August, Peter Dixon, Nick Hasell, Lindsay Wallace, Kings Langley, David Hands Rugby Correspondent, Tom Dart, Ingrid Mansell and Dominic Walsh, Peter Hardy, James Doran, Melissa Kite Political Correspondent, Christopher Walker, Helen Franks, Ian Johns, Mel Webb, Des Dearlove, Ian Cobain and Dalya Alberge, Richard Heller, Russell Jenkins, Alan Lee, Peter Riddell, Ben MacIntyre, Lea Paterson Economics Editor, Michael Gove, Tony Dawe, Philip Webster, Jack Malvern Arts Reporter, Lea Paterson, Gary Duncan Economics Correspondent, Mark Henderson, Martin Waller, James Jackson, Carl Mortished International Business Editor, Laura Peek, Benedict Nightingale, Martin Amis, Dr Thomas Stuttaford, Stephen Dalton, Steve Allen, Leo Lewis, Philip Rogers, James Christopher, Gordon Brown, Alan Lee Racing Correspondent, Manish Agarwal, Robbie Millen, Edward Gorman Sailing Correspondent, Sabrina Wolfe, Mark Bristow, Christopher Irvine, Michael Heath, A. Graham Hellier,
... The T-shirts of peace You monkey, you minion - shut up and sit down Ministers from Irag ...
2003 - Gale Group | TDA
Christian Grumaz, Anne Hoffmann, Yevhen Vainshtein, Maria Kopp, Silke Grumaz, Philip Stevens, Sebastian Decker, Markus A. Weigand, Stefan Hofer, Thorsten Brenner, Kai Sohn,
... would have also been detected using the optimized MinION workflow. Reliable identification of pathogens based on circulating ... would have also been detected using the optimized MinION workflow. Reliable identification of pathogens based on circulating ... most recent sequencing platforms is represented by the MinION (Oxford Nanopore Technologies, Oxford, UK), a handheld sequencer ... M. A first look at the Oxford Nanopore MinION sequencer.Mol Ecol Resour. 2014; 14: 1097-1102Crossref ... P. Hall L.J. Clark M.D. Rapid MinION metagenomic profiling of the preterm infant gut microbiota ...
Tópico(s): Genomics and Phylogenetic Studies
2020 - Elsevier BV | Journal of Molecular Diagnostics
Rev. A. Pierce-Jones, Ruth Rouse, Stephen Charles Neill, S. C. Carpenter, Geddes MacGregor, Harry Blamires, G. E. Wright, Karl Barth, Hugh Martin, A. Pierce-Jones, A. C. Craig, T. Price, Norman J. Bull,
... M. 5), The Progress of 'Jane', The Pitt Minion Reference Bible, The Church and Racial Tensions World ...
1954 - Gale Group | NCCO EuropeAfrica
Andrea Maurizio Cabibbe, Andrea Spitaleri, Simone Battaglia, Rebecca E. Colman, Anita Suresh, Swapna Uplekar, Timothy C. Rodwell, Daniela María Cirillo,
... on the compact, low-cost Oxford Nanopore Technologies MinION sequencer. One hundred four DNA samples extracted from ... assay on an Illumina MiniSeq, were resequenced on MinION after applying a custom library preparation. MinION read quality, mapping statistics, and variant calling were ... the reference MiniSeq data. The average percentage of MinION reads mapped to an H37RV reference genome was ... coverage were 4,151× and 4,177× on MinION and MiniSeq, respectively, with heterogeneous distribution across targeted ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2020 - American Society for Microbiology | Journal of Clinical Microbiology
Victoria McKee, Rosemary Righter, Chris Petit, Paul Heiney, Graham Rock, Clifford Longley, Patricia Davies, John Vincent, Jane MacQuitty, Simon Barnes, Stephen Leather, Tim Judah, Patrick Hanratty, Gordon Allan, Neil Bennett, Barbara Ellis, Michael Seely, Racing Correspondent, Clive Scott-Hopkins, Philip Howard, Nick Worrall, Laura Thompson, Tom Giles, Jeremy Kingston, Clive Davis, Melinda Wittstock, Media Correspondent, Carol Leonard, Albert Dormer, Marion Shoard, Richard Streeton, Anthoyn Quinton, James Bone and Michael Knipe, Geoff Brown, Ray Clancy, D. L. Froomberg, Chairman, Francesca Greenoak, Martin Fletcher, Andrew Wadsworth, K. W. Ruddiman, Principal, Stuart Jones, Peter Jones, Michael Chadwick, Michael Bryant, Louise Taylor, Huon Mallalieu, John Varney, Peter Bills, Michael Evans, Defence Correspondent, Robin Young, Brian Morton, Jenny MacArthur, Philip Robinson, John Young, Douglas Broom, Local Government Correspondent, Frances Bissell, David Hart, General Secretary, Ian Bradley, Norman Hammond Archaeology Correspondent, John Percival, John Hennessy, Quentin Cowdry Home Affairs Correspondent, Wolfgang M?nchau, Harvey Elliott, Air Correspondent, Richard Basset, Nicole Swengley, George Melly, S. W. Morris, Gavin Bell, John Woodcock, Lindsay Cook, Edward Gorman, Irish Affairs Correspondent, Michael Theodoulou, Robert Kirley, Wolfgang Munchau, Lindsay Cook Money Editor, Robin Allen, Rupert Bruce, Gillian Bowditch, Geoffrey Wheeler, Michael Tate, Deputy City Editor, Christopher Lloyd, Simon Burrough, Sandy Bisp, John Higgins, Catherine Sampson, Marcus Binney, Kerry Gill, John Russell Taylor, John Grigg, Barbara Amiel, Kunwar Khalid Yonus, Craig Brown, Paul Griffiths, D. V. Q. Henriques, Stephen Thorpe, Ken Lawrence, John Blunsden, Richard Owen, Eric Moonman, Chairman, Keith Wheatley, Nigel Hawkes, Science Editor, Keith MacKlin, Rodney Hobson, Tim Jones and Kevin Eason, Christopher Thomas, Christopher Walker, Clive White, Sebastian Coe, Matthew Parris, Graham Searjeant Financial Editor, Liz Smith, Alan Franks, Jonathan Clark, John Shaw, Ricardo Dorich, Thomson Prentice, Medical Correspondent, Jim Stather, Alan Lee Cricket Correspondent, John Julius Norwich, Graham Searjeant, David Sinclair, Lindsay Cook Weekend Money Editor, Alan McGregor, Benedict Nightingale, Colin Campbell and Michael Tate, Barry Pickthall, Yachting Correspondent, Jonathan Meades, Lulu Yu, Jack Bailey, Richard Morrison, Jennifer Macrory (Librarian), Derek Harris Industrial Editor, Juan Carlos, Matthew Bond, James Bone and Michael Knipe, Diplomatic Correspondent, Colin Campbell, Susan Ellicott, Shona Crawford Poole, Judy Froshaug, George Lloyd's, Mary Dejevsky, Dennis Walters,
... send 'rescue us' plea Kuwait City Bitter-sweet Minion as detained son returns to his family Thatcher ...
1990 - Gale Group | TDA
Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González‐Escalona, David L. Erickson, Eric W. Brown, Jianghong Meng,
... n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9. ... potential, whole-genome phylogeny, and pan-genome. The MinION reads were base-called in real-time using ... 0.4.8 were used to generate the MinION, MiSeq, and hybrid assemblies, respectively. The MinION assemblies were highly contiguous compared to the MiSeq ... which corrected erroneous single nucleotide polymorphisms (SNPs). The MinION assemblies provided similar predictions of AMR and virulence ... tetracycline resistance genes in 11 of the 19 MinION assemblies of tetracycline-resistant isolates. The MinION assemblies ...
Tópico(s): Bacterial Identification and Susceptibility Testing
2020 - Public Library of Science | PLoS ONE
Daniel Harris, G. Smith, Greville Brooke,
... Classified Advertising Items The new book, entirled "The Minion of the… Multiple Classified Advertising Items Dr. La' ...
1850 - Gale Group | Sunday Times HA GDA
William Taylor, John F. Pearson, Allison L. Miller, Sebastian Schmeier, Frank Frizelle, Rachel Purcell,
... variety of methods. In this study, we compare MinION sequencing with meta-transcriptomics and amplicon-based sequencing ... colorectal tumour samples. 16S rRNA amplicon sequencing and MinION sequencing was carried out using genomic DNA, and RNA-Sequencing for meta-transcriptomic analysis. Non-human MinION and RNA-Sequencing reads, and 16S rRNA amplicon ... was greater than 129 times that generated using MinION sequencing. However, the average read length of MinION sequences was more than 13 times that of ...
Tópico(s): Oral microbiology and periodontitis research
2020 - Public Library of Science | PLoS ONE
... the Arena Chapter XXXIV How Nero Gave His Minion a Terrible Fright, That Rascal Jack Chapter XXXI ...
1885 - Gale Group | NCCO British Theatre
Nguyen Thi Thu Hong, Ho Dang Trung Nghia, Tran Tan Thanh, Nguyen Phu Huong Lan, Nguyen Thi Han Ny, Nghiêm My Ngoc, Vu Thi Ty Hang, Le Thi My Chau, Van Xuan Quynh, Le Thi Diem, Bui Thi Bich Hanh, Nguyen Ho Hong Hanh, Du Trong Duc, Dinh Nguyen Huy Man, James I. Campbell, Pham Kieu Nguyet Oanh, Jeremy Day, Nguyen Hoan Phu, Nguyễn Văn Vĩnh Châu, Guy Thwaites, Le Van Tan,
... pcr amplification of 16S rRNA genes using the MinION™ nanopore sequencer.FEBS Open Bio. 2019; 9: 548- ... by a 16S rRNA gene analysis using the MinION nanopore sequencer, South Korea, 2016.Emerg Microbes Infect. ... patient was discharged with full clinical recovery. Additionally, MinION sequencing of complete 16S rRNA gene was retrospectively ... them and the sequencing errors introduced by the MinION systems. Analysis of sequencing data generated during the ... same results (Supplementary Figure 2). Details about the MinION procedure are presented in Supplementary Materials. To further ...
Tópico(s): Genomics and Phylogenetic Studies
2019 - Elsevier BV | Journal of Infection
Maureen C. Montgomery, Chang Liu, Rosanne Petraroia, Eric T. Weimer,
... However, to demonstrate feasibility, the utility of the MinION sequencer (Oxford Nanopore Technologies, Oxford, UK) was evaluated ... However, to demonstrate feasibility, the utility of the MinION sequencer (Oxford Nanopore Technologies, Oxford, UK) was evaluated ... Full-length HLA class I genotyping with the MinION nanopore sequencer.Methods Mol Biol. 2018; 1802: 155- ... Full-length HLA class I genotyping with the MinION nanopore sequencer.Methods Mol Biol. 2018; 1802: 155- ... Fisher Scientific, Carlsbad, CA). To increase the downstream MinION sequencing output, one sample (cDNA014) was further purified ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - Elsevier BV | Journal of Molecular Diagnostics
Kaire Loit, Kalev Adamson, Mohammad Bahram, Rasmus Puusepp, Sten Anslan, Riinu Kiiker, Rein Drenkhan, Leho Tedersoo,
... Of the third-generation sequencing techniques, the portable MinION device (Oxford Nanopore Technologies) has received much attention ... relative performances of two third-generation sequencing instruments, MinION and Sequel (Pacific Biosciences), in identification and diagnostics ... is efficient for metabarcoding of complex samples, whereas MinION is not suited for this purpose due to ... rate and multiple biases. However, we find that MinION can be utilized for rapid and accurate identification ... extraction and incubation times, we performed the entire MinION workflow, from sample preparation through DNA extraction, sequencing, ...
Tópico(s): Plant Disease Resistance and Genetics
2019 - American Society for Microbiology | Applied and Environmental Microbiology
Jamie L. Duke, Timothy L. Mosbruger, Deborah Ferriola, Nilesh Chitnis, Taishan Hu, Nikolaos Tairis, David J. Margolis, Dimitri Monos,
... of ambiguities. In this study, the Oxford Nanopore MinION, a third-generation sequencing technology, was used to ... combined with the long reads obtained from the MinION platform, allow for the resolution of the tested ... of ambiguities. In this study, the Oxford Nanopore MinION, a third-generation sequencing technology, was used to ... combined with the long reads obtained from the MinION platform, allow for the resolution of the tested ... together with Oxford Nanopore Technology (ONT; Oxford, UK) MinION sequencing offers a way to accurately resolve ambiguities ...
Tópico(s): T-cell and B-cell Immunology
2019 - Elsevier BV | Journal of Molecular Diagnostics
MarkC Preul, Arpan Patel, Evgenii Belykh, Eric J Miller, LaethL George, NikolayL Martirosyan, Vadim A. Byvaltsev,
... paper reviews nanopore sequencing, an innovation utilized by MinION and outlines its potential use for neurosurgery.Methods: ... conducted for publications containing the keywords of Oxford MinION, nanopore sequencing, brain tumor, glioma, whole genome sequencing ( ... and used for this review.Results: The Oxford MinION nanopore sequencing technology has had successful applications within ... specialties.Technical details, methodology, and current use of MinION sequencing are discussed through the prism of potential ...
Tópico(s): Glioma Diagnosis and Treatment
2018 - Medknow | Surgical Neurology International
Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Kotaro Mitsutake, Takashi Murakami, Shigefumi Maesaki, Takuya Maeda,
... for influenza viruses in clinical specimens with the MinION portable sequencer. Methods: Whole genomes of influenza A ... collected in Tokyo, Japan. Barcode tags for multiplex MinION sequencing were added with each multiplex RT-PCR ... amplicons were mixed and multiplex sequencing using the MinION sequencer with 1D2 sequencing kit. In addition, multiplex ... Illumina MiSeq platform to validate the performance of MinION sequencer. The accuracy, recall, and precision rates of MinION sequencing were calculated by comparing the results of ...
Tópico(s): vaccines and immunoinformatics approaches
2018 - Frontiers Media | Frontiers in Microbiology
Bonnie L. Brown, Mick Watson, Samuel S. Minot, Maria C. Rivera, Rima B. Franklin,
... and biological factors. A nanopore-based sequencing platform, MinION™, produces reads that are ≥1 × 104 bp in ... tested the ability of sequence data produced by MinION (R7.3 flow cells) to correctly assign taxonomy ... low-complexity communities was assessed by analyzing the MinION sequence data with three different bioinformatic approaches: Kraken, ... 5 kit and chemistry, run on the original MinION device, yielded as few as 224 to as ... correctly assigned to the species level. Synthetic metagenome MinION libraries generated using version 6 kit and chemistry ...
Tópico(s): RNA modifications and cancer
2017 - University of Oxford | GigaScience
Kayzad Nilgiriwala, Marie Sylvianne Rabodoarivelo, Michael B. Hall, Grishma Patel, Ayan Mandal, Shefali Mishra, Fanantenana Randria Andrianomanana, Kate E. Dingle, Gillian Rodger, Sophie George, Derrick W. Crook, Sarah Hoosdally, Nerges Mistry, Niaina Rakotosamimanana, Zamin Iqbal, Simon Grandjean Lapierre, Timothy M. Walker,
... were included. Sequencing was performed using Illumina and MinION (monoplex and multiplex) technologies. We measured the impact ... samples using Illumina, 34/38 were identified using MinION-monoplex sequencing, and 20/24 were identified using MinION-multiplex sequencing. The fraction of M. tuberculosis reads from MinION sequencing was lower than from Illumina, but monoplexing grade 3+ samples on MinION produced higher read depth than Illumina (P < 0.05) and MinION multiplexing (P < 0.01). No significant differences in ...
Tópico(s): Genomics and Phylogenetic Studies
2023 - American Society for Microbiology | Journal of Clinical Microbiology
Anja Pecman, Ian P. Adams, Ion Gutiérrez‐Aguirre, Adrian Fox, Neil Boonham, Maja Ravnikar, Denis Kutnjak,
... HTS platform (MiSeq benchtop sequencer-Illumina), with the MinION sequencer (Oxford Nanopore Technologies) for the detection of ... depleted total RNA (rRNA-depleted totRNA) and for MinION sequencing, direct RNA sequencing of totRNA was used. ... viroid, three additional variations of sample preparation for MinION sequencing were also used: direct RNA sequencing of ... sample at an extremely low titer. All other MinION sequencing approaches showed improved performance with outcomes similar ... totRNA showing the best performance amongst tested nanopore MinION sequencing approaches. Moreover, when enough sequencing data were ...
Tópico(s): Plant and Fungal Interactions Research
2022 - Frontiers Media | Frontiers in Microbiology
Lucky Ronald Runtuwene, Josef S. B. Tuda, Arthur E. Mongan, Yutaka Suzuki,
... level with the advent of Oxford Nanopore Technologies’ MinION. The low equipment investment, ease of library preparation, ... computer enable the portability for on-site sequencing. MinION has had its role in clinical, biosecurity, and ... the many facets of on-site sequencing with MinION. First, we will present some field works using MinION. We will discuss the requirements for targeted or ... procedures available for data analysis in the field. MinION has greatly changed the way we do sequencing ... to the biodiversity. Although numerous limitations exist for MinION to be truly portable, improvements of procedures and ...
Tópico(s): Genomics and Rare Diseases
2019 - Springer Nature | Advances in experimental medicine and biology
Spyros Oikonomopoulos, Yu Chang Wang, Haig Djambazian, Dunarel Badescu, Jiannis Ragoussis,
... assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, cDNAs from the External RNA Controls ... the direct comparison between different sequencing platforms. The MinION performance was assessed for its ability to sequence ... abundance of the ERCC cDNA molecules sequenced by MinION agreed with their expected concentration. No length or ... Illumina HiSeq 2500, PacBio RS II and ONT MinION platforms. We observed that there was a good ... cDNA abundance between PacBio RS II and ONT MinION (r pearson = 0.82, isoforms with length more ...
Tópico(s): CRISPR and Genetic Engineering
2016 - Nature Portfolio | Scientific Reports
... The current study attempts to analyse and compare MinION sequencing data with Ion Torrent data, using a ... five samples on both Ion Torrent and Oxford MinION platforms. The resulting data were analysed for output, ... variant metrics across both platforms independently. Results Overall, MinION produced longer reads (range 108‐7227 bp) than ... lower error rate of 1.59% compared with MinION's 20.31%. Despite high error, MinION platform was able to identify and report the ... and Conclusion The extremely long read lengths of MinION sequencers and better coverage in difficult to sequence ...
Tópico(s): Genomics and Rare Diseases
2020 - Wiley | International Journal of Laboratory Hematology
Nina Vasiljevic, Marisa Lim, Emily Humble, Adeline Seah, Adelgunde Kratzer, Nadja V. Morf, Stefan Prost, Rob Ogden,
... are analysed simultaneously. The Oxford Nanopore Technologies (ONT) MinION™ is an affordable and small footprint DNA sequencing ... high-throughput (deep read) sequence data from the MinION making it currently impractical for many wildlife forensic end-users. Here, we present a MinION deep read sequence data validation study for species ... we investigated the impact of differences between the MinION consensus and Sanger control sequences on correct species ... to understand the ability and accuracy of the MinION consensus sequence to differentiate the true species from ...
Tópico(s): Genomics and Phylogenetic Studies
2021 - Elsevier BV | Forensic Science International Genetics
Joshua Quick, Aaron R. Quinlan, Nicholas J. Loman,
The MinION™ is a new, portable single-molecule sequencer developed by Oxford Nanopore Technologies. It measures four inches in ... 3.0 port of a laptop computer. The MinION™ measures the change in current resulting from DNA ... coli K-12 substr. MG1655 generated on a MinION™ device during the early-access MinION™ Access Program (MAP). Sequencing runs of the MinION™ are presented, one generated using R7 chemistry (released ... demonstrate the nature of data produced by the MinION™ platform and to encourage the development of customised ...
Tópico(s): RNA modifications and cancer
2014 - University of Oxford | GigaScience
Ketema Tafess, Timothy Ting-Leung Ng, Hiu Yin Lao, Kenneth Siu Sing Leung, Kingsley King Gee Tam, Rahim Rajwani, Sarah Tsz Yan Tam, Lily Pui Ki Ho, Corey Mang Kiu Chu, Dimitri Gonzalez, Chalom Sayada, Oliver Chiu Kit, Belete Haile, Gobena Ameni, Wing Cheong Yam, G. G. Siu,
... sequencing workflows based on Illumina MiSeq and Nanopore MinION for the prediction of drug resistance in M. ... barcoded and sequenced in parallel on MiSeq and MinION in respective batch sizes of 24 and 12 ... 649×, respectively. The variant calling by MiSeq and MinION could achieve 100% agreement if variants with an allele frequency of <40% reported by MinION were excluded. Both workflows achieved a mean clinical ... test (pDST). Turnaround times for the MiSeq and MinION workflows were 38 and 15 h, facilitating the ...
Tópico(s): Genomics and Phylogenetic Studies
2020 - American Association for Clinical Chemistry | Clinical Chemistry