Produção Nacional
Revisado por pares
Yollanda Edwirges Moreira Franco, Maria José Ferreira Alves, Miyuki Uno, Isabele Fattori Moretti, Marina Trombetta-Lima, Suzana de Siqueira Santos, Ancély Ferreira dos Santos, Gabriel Santos Arini, Maurı́cio S. Baptista, Antônio Marcondes Lerário, Sueli Mieko Oba‐Shinjo, Suely Kazue Nagahashi Marie,
Abstract Background Glioblastoma is the most frequent and high-grade adult malignant central nervous system tumor. The prognosis is still poor despite the use of combined therapy involving maximal surgical resection, radiotherapy, and chemotherapy. Metabolic reprogramming currently is recognized as one of the hallmarks of cancer. Glutamine metabolism through glutaminolysis has been associated with tumor cell maintenance and survival, and with antioxidative stress through glutathione (GSH) synthesis. ...
Tópico(s): Epigenetics and DNA Methylation
2021 - BioMed Central | Cancer & Metabolism
Produção Nacional
Revisado por pares
Marina Trombetta-Lima, Lívia Rosa-Fernandes, Cláudia B. Angeli, Isabele Fattori Moretti, Yollanda Edwirges Moreira Franco, Adaliana Sorg Mousessian, Alda Wakamatsu, Antônio Marcondes Lerário, Sueli Mieko Oba‐Shinjo, Carlos Augusto Pasqualucci, Suely Kazue Nagahashi Marie, Giuseppe Palmisano,
Medulloblastomas (MBs) and glioblastomas (GBMs) are high-incidence central nervous system tumors. Different origin sites and changes in the tissue microenvironment have been associated with the onset and progression. Here, we describe differences between the extracellular matrix (ECM) signatures of these tumors. We compared the proteomic profiles of MB and GBM decellularized tumor samples between each other and their normal decellularized brain site counterparts. Our analysis revealed that 19, 28, ...
Tópico(s): Ferroptosis and cancer prognosis
2021 - American Chemical Society | Journal of Proteome Research
Produção Nacional
Revisado por pares
Elisa Frederico Seneme, Daiane Carla dos Santos, Evelyn Marcela Rodrigues Silva, Yollanda Edwirges Moreira Franco, Giovanna Barbarini Longato,
Natural products have been used by humanity for many centuries to treat various illnesses and with the advancement of technology, it became possible to isolate the substances responsible for the beneficial effects of these products, as well as to understand their mechanisms. In this context, myristicin, a substance of natural origin, has shown several promising activities in a large number of in vitro and in vivo studies carried out. This molecule is found in plants such as nutmeg, parsley, carrots, ...
Tópico(s): Traditional and Medicinal Uses of Annonaceae
2021 - Multidisciplinary Digital Publishing Institute | Molecules
Produção Nacional
Revisado por pares
Pedro Augusto Alves, Ellen G. de Oliveira, Ana Paula Moreira Franco-Luiz, Letícia Trindade Almeida, Amanda Bonoto Gonçalves, Iara A. Borges, Flávia Rocha, Raíssa Prado Rocha, Matheus Filgueira Bezerra, Pâmella Miranda, Flávio Diniz Capanema, Henrique Resende Martins, Gerald Weber, Santuza Maria Ribeiro Teixeira, Gabriel Luz Wallau, Rubens Lima do Monte‐Neto,
The coronavirus disease 2019 (COVID-19) pandemic unfolded due to the widespread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission reinforced the urgent need for affordable molecular diagnostic alternative methods for massive testing screening. We present the clinical validation of a pH-dependent colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection. The method revealed a limit of detection of 19.3 ± 2.7 viral genomic copies/ ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2021 - Frontiers Media | Frontiers in Microbiology
Produção Nacional
Revisado por pares
Yollanda Edwirges Moreira Franco, Márcia Y. Okubo, Adriana de la Torre, Paula Pereira de Paiva, Marcela N. Rosa, Viviane Aline Oliveira Silva, Rui Manuel Reis, Ana Lúcia Tasca Góis Ruiz, Paulo M. Imamura, João Ernesto de Carvalho, Giovanna Barbarini Longato,
Glioblastoma (GBM) is the most frequent and highest-grade brain tumor in adults. The prognosis is still poor despite the use of combined therapy involving maximal surgical resection, radiotherapy, and chemotherapy. The development of more efficient drugs without noticeable side effects is urgent. Coronarin D is a diterpene obtained from the rhizome extract of Hedychium coronarium, classified as a labdane with several biological activities, principally anticancer potential. The aim of the present ...
Tópico(s): Natural Compounds in Disease Treatment
2019 - Multidisciplinary Digital Publishing Institute | Molecules
Blossom Akagbosu, Zakieh Tayyebi, Gayathri Shibu, Yoselin A. Paucar Iza, Deeksha Deep, Yollanda Edwirges Moreira Franco, Logan Fisher, H. Amalia Pasolli, Valentin Thevin, Rasa Elmentaite, Maximilian M. L. Knott, Saskia Hemmers, Lorenz Jahn, Christin Friedrich, Jacob Verter, Zhong-Min Wang, Marcel R.M. van den Brink, Georg Gasteiger, Thomas G. P. Grünewald, Julien C. Marie, Christina Leslie, Alexander Y. Rudensky, Chrysothemis C. Brown,
Abstract Establishing and maintaining tolerance to self-antigens or innocuous foreign antigens is vital for the preservation of organismal health. Within the thymus, medullary thymic epithelial cells (mTECs) expressing autoimmune regulator (AIRE) have a critical role in self-tolerance through deletion of autoreactive T cells and promotion of thymic regulatory T (T reg ) cell development 1–4 . Within weeks of birth, a separate wave of T reg cell differentiation occurs in the periphery upon exposure to antigens ...
Tópico(s): T-cell and B-cell Immunology
2022 - Nature Portfolio | Nature
Produção Nacional
Revisado por pares
Andreia Elisa Cursino, Ana Paula Pessoa Vilela, Ana Paula Moreira Franco-Luiz, Jaquelline Germano de Oliveira, Márcia Furlan Nogueira, João Pessoa Araújo Júnior, Daniel Moura de Aguiar, Erna Geessien Kroon,
Equine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those ...
Tópico(s): Viral Infections and Vectors
2018 - Springer Science+Business Media | Archives of Virology
Produção Nacional
Revisado por pares
Tatiana Paiva Moreira Franco, Ana Paula Pires dos Santos, Antônio Canabarro,
Tópico(s): Laser Applications in Dentistry and Medicine
2018 - Springer Science+Business Media | Lasers in Medical Science
Produção Nacional
Revisado por pares
Maria Elisa Melo Branco de Araújo, Yollanda Edwirges Moreira Franco, Thiago Grando Alberto, Márcia Cristina Fernandes Messias, Camila Wielewski Leme, Alexandra Christine Helena Frankland Sawaya, Patrícia de Oliveira Carvalho,
Studies have reported that flavonoids inhibit xanthine oxidase (XO) activity; however, poor solubility and stability in lipophilic media limit their bioavailability and applications. This study evaluated the kinetic parameters of XO inhibition and partition coefficients of flavonoid esters biosynthesised from hesperidin, naringin, and rutin via enzymatic acylation with hexanoic, octanoic, decanoic, lauric, and oleic acids catalysed by Candida antarctica lipase B (CALB). Quantitative determination ...
Tópico(s): Natural product bioactivities and synthesis
2017 - Taylor & Francis | Journal of Enzyme Inhibition and Medicinal Chemistry
Produção Nacional
Revisado por pares
Danilo Bretas de Oliveira, Talitah Michel Sanchez Candiani, Ana Paula Moreira Franco-Luiz, Gabriel Magno de Freitas Almeida, Jônatas Santos Abrahão, María Rios, Roney S. Coimbra, Erna Geessien Kroon,
Meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. The range of viral meningitis severity depends mainly on age, immune status and etiological agent. The aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. The diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (CSF) of patients. ...
Tópico(s): Viral Infections and Vectors
2017 - Elsevier BV | Journal of the Neurological Sciences
Produção Nacional
Revisado por pares
Maria de Araújo, Yollanda Edwirges Moreira Franco, Márcia Cristina Fernandes Messias, Giovanna Barbarini Longato, João Alencar Pamphile, Patrícia de Oliveira Carvalho,
Several studies have described important biological activities of flavonoids such as coronary heart disease prevention, hepatoprotective, anti-inflammatory and anticancer activities, enzyme inhibition activity, and antibacterial, antifungal, and antiviral activities. Flavonoids show promising activity as natural plant-based antioxidants due to their antioxidant and free radical scavenging properties. However, their primary applications as antioxidants in the pharmaceutical, cosmetic, and food industries ...
Tópico(s): Natural Antidiabetic Agents Studies
2016 - Thieme Medical Publishers (Germany) | Planta Medica
Ana Paula Moreira Franco-Luiz, Alexandre Fagundes-Pereira, Galileu Barbosa Costa, Pedro Augusto Alves, Danilo Bretas de Oliveira, Cláudio Antônio Bonjardim, Paulo César Peregrino Ferreira, Giliane de Souza Trindade, Carlos Javier Panei, Cecília Mônica Galosi, Jônatas Santos Abrahão, Erna Geessien Kroon,
9).The TMB-2-producing A. soli strain that we isolated came from a blood culture, indicating that A. soli is a potential cause of bloodstream infections or bacteremia. A. soli has also been detected in lice and keds of domestic animals (10), indicating that A. soli may inhabit natural environments and that injuries and bites by arthropods might present a risk for invasive infections.Isolates of Acinetobacter species, particularly those recovered from blood culture, should be identified to species ...
Tópico(s): Animal Disease Management and Epidemiology
2014 - Centers for Disease Control and Prevention | Emerging infectious diseases
Produção Nacional
Revisado por pares
Fábio P. Dornas, Lorena C. F. Silva, Gabriel Magno de Freitas Almeida, Rafael K. Campos, Paulo Victor de Miranda Boratto, Ana Paula Moreira Franco-Luiz, Bernard La Scola, Paulo César Peregrino Ferreira, Erna Geessien Kroon, Jônatas Santos Abrahão,
Viruses are extremely diverse and abundant and are present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around the world. As evidence of their ubiquity and ecological impact, mimiviruses have been found in multiple environmental samples. However, isolation of these viruses from environmental samples is inefficient, mainly due to methodological limitations and lack of information regarding the interactions between viruses ...
Tópico(s): Legionella and Acanthamoeba research
2014 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Maria Elisa Melo Branco de Araújo, Yollanda Edwirges Moreira Franco, Thiago Grando Alberto, Mariana Alves Sobreiro, Marco Aurélio Veiga Conrado, Denise Gonçalves Priolli, Alexandra Christine Helena Frankland Sawaya, Ana Lúcia Tasca Góis Ruiz, João Ernesto de Carvalho, Patrícia de Oliveira Carvalho,
Bioavailability and biological properties of flavonoid glycosides can be improved after the enzymatic hydrolysis of specific glycosyl groups. In this study, we evaluate the antioxidant and antiproliferative potential of rutin after enzymatic hydrolysis performed by α-l-rhamnosidases (hesperidinase from Penicillium sp. and naringinase from Penicillium decumbens) previously heated at 70°C for 30 min to inactivate the undesirable β-d-glucosidase activity. The highest in vitro antioxidant activity determined ...
Tópico(s): Natural product bioactivities and synthesis
2013 - Elsevier BV | Food Chemistry
Produção Nacional
Revisado por pares
Cecília Luiza Simões Santos, Maria Anice Mureb Sallum, Heitor Moreira Franco, Fabíola M. Oshiro-Monreal, Iray Maria Rocco,
The molecular characterization of SPH253157, a new strain of St. Louis encephalitis virus (SLEV), isolated in 2004 from the first case of human infection recognized in the state of São Paulo, Brazil, is reported. The patient, presenting a febrile illness without neurological involvement, was hospitalized as a probable case of dengue fever. Genomic RNA was isolated from the supernatant of C6/36 cells infected with acute phase-serum specimen of the patient and the envelope gene was amplified by reverse- ...
Tópico(s): Malaria Research and Control
2006 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Rosângela Rodrigues, Luciene Cardoso Scherer, Cristina Mendes de Oliveira, Heitor Moreira Franco, Rosa Dea Sperhacke, João Leandro Ferreira, Simone Martins de Castro, Isete Maria Stella, Luís Fernando de Macedo Brígido,
We describe preliminary molecular characterization of HIV-1 pol from 108 consecutive HIV seropositive users of a Voluntary Counseling and Testing (VCT) site of Porto Alegre city, the major metropolitan area in the south of Brazil. Protease and partial reverse transcriptase regions were retrotranscribed from plasma HIV-1 RNA and sequenced after direct nested PCR. Principal antiretroviral resistance mutations (ARM) were observed in 3% of the samples, two cases with K103N and one with M41L, L210W and ...
Tópico(s): HIV/AIDS Research and Interventions
2005 - Elsevier BV | Virus Research
Produção Nacional
Revisado por pares
Luís Fernando de Macedo Brígido, Heitor Moreira Franco, Renata Marconi Custódio, Carmen A. Freitas Oliveira, João Leandro Ferreira, Margareth da Eira, Fernando Bergel, Fabio Araújo, Jose R. Carvalheiro, Rosângela Rodrigues,
Partial sequences of HIV-1 polymerase from 185 patients, 141 ARV experienced and 44 naive, of gag (p24) and env (C2V3) from a subset of naive cases were evaluated in São Paulo, Brazil. Antiretroviral resistance mutations were detected in 4% of 26 recently (<2 years) infected patients. Polymorphisms at the protease gene were common both in contemporary and pre-HAART era isolates, some significantly associated with the viral clade. HIV-1 clade B was preponderant, in 79%, with 11% clade F and one case ...
Tópico(s): HIV/AIDS Research and Interventions
2005 - Mary Ann Liebert, Inc. | AIDS Research and Human Retroviruses
Produção Nacional
Revisado por pares
Wilson Malagó, Heitor Moreira Franco, Euclides Matheucci, Adriana Medaglia, Flávio Henrique‐Silva,
Ostrich (Struthio camelus) breeds have been gaining increasing significance around the world. The large-scale sex determination of chicks is an important task in the development of these breeds. To date, two PCR-based methods have been established for ostrich sex typing, neither of them intended for large-scale analyses. Here, we report on a protocol adapted to carry out large-scale gender scoring using DNA obtained from chick feathers. The DNA was extracted using a fast and simple alkaline extraction ...
Tópico(s): Bird parasitology and diseases
2002 - BioMed Central | BMC Biotechnology
René Drucker-Colı́n, Ignacio Madrazo, Feggy Ostrosky‐Solís, M Shkurovich, Rebecca Moreira Franco, Cesar Lara - Torres,
Tópico(s): Nerve injury and regeneration
1988 - Elsevier BV | Progress in brain research
Produção Nacional
Revisado por pares
Felipe Lopes de Assis, Ana Paula Moreira Franco-Luiz, Raíssa Nunes dos Santos, Fabrício Souza Campos, Fábio P. Dornas, Paulo V. M. Borato, Ana Cláudia Franco, Jônatas Santos Abrahão, Philippe Colson, Bernard La Scola,
The family Mimiviridae, comprised by giant DNA viruses, has been increasingly studied since the isolation of the Acanthamoeba polyphaga mimivirus (APMV), in 2003. In this work, we describe the genome analysis of two new mimiviruses, each isolated from a distinct Brazilian environment. Furthermore, for the first time, we are reporting the genomic characterization of mimiviruses of group C in Brazil (Br-mimiC), where a predominance of mimiviruses from group A has been previously reported. The genomes ...
Tópico(s): Viral gastroenteritis research and epidemiology
2017 - Frontiers Media | Frontiers in Microbiology
Ana Paula Moreira Franco-Luiz, Danilo Bretas de Oliveira, Alexandre Fagundes Pereira, Mirela Cristina Soares Gasparini, Cláudio Antônio Bonjardim, Paulo César Peregrino Ferreira, Giliane de Souza Trindade, Rodrigo Puentes, Agustín Furtado, Jônatas Santos Abrahão, Erna Geessien Kroon,
Abstract We detected orthopoxvirus in 28 of 125 serum samples collected during 2009 from cattle in Uruguay. Two samples were PCR-positive for vaccinia virus and had sequences similar to those for vaccinia virus associated with outbreaks in Brazil. Autochthonous circulation of vaccinia virus in Uruguay and other South American countries cannot be ruled out.
Tópico(s): Virus-based gene therapy research
2016 - Centers for Disease Control and Prevention | Emerging infectious diseases
Produção Nacional
Revisado por pares
Maria Elisa Melo Branco de Araújo, Fabiano Jares Contesini, Yollanda Edwirges Moreira Franco, Alexandra Christine Helena Frankland Sawaya, Thiago Grando Alberto, Natália Dalfré, Patrícia de Oliveira Carvalho,
Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin ...
Tópico(s): Plant biochemistry and biosynthesis
2011 - Multidisciplinary Digital Publishing Institute | Molecules
Produção Nacional
Revisado por pares
Yollanda Edwirges Moreira Franco, Carolina A. de Lima, Marcela N. Rosa, Viviane Aline Oliveira Silva, Rui Manuel Reis, Denise Gonçalves Priolli, Patrícia de Oliveira Carvalho, Jessyane Rodrigues do Nascimento, Cláudia Quintino da Rocha, Giovanna Barbarini Longato,
Recently, many studies have reported the anticancer properties of flavonoid luteolin against a variety of tumors, but there is still a lack in the description of its mechanism of action. In attempt to better contribute to the literature, we evaluated the antiproliferative activity of luteolin extracted by Fridericia platyphylla in a panel of tumor cell lines representative of six different tissues. Luteolin presented antiproliferative activity for all the assessed tumor cell lines, being glioblastoma ...
Tópico(s): Traditional Chinese Medicine Analysis
2020 - Taylor & Francis | Natural Product Research
Produção Nacional
Revisado por pares
Débora Jacomini, Susana Moreira, Franco Dani Campos Pereira, Ricardo de Lima Zollner, Márcia Regina Brochetto-Braga,
Tópico(s): Contact Dermatitis and Allergies
2014 - Elsevier BV | Toxicon
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Ynara Bosco de Oliveira Lima‐Arsati, Camila Gobo de Freitas, Vera Cavalcanti de Araújo,
The purpose of this study was to investigate the responses of salivary immunoglobulin A (SIgA) in 10 professional top-level Brazilian futsal players after 2 highly competitive games separated by 7 days. Unstimulated saliva was collected over a 5-minute period at PRE- and POST-match. The SIgA was measured by an enzyme-linked immunosorbent assay and expressed as the absolute concentration (SIgAabs) and secretion rate of IgA (SIgArate). Rate of perceived exertion and heart rate were used to monitor the ...
Tópico(s): Sports Performance and Training
2011 - Lippincott Williams & Wilkins | The Journal of Strength and Conditioning Research
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Ynara Bosco de Oliveira Lima‐Arsati, Antônio Carlos Simões, Vera Cavalcanti de Araújo,
Abstract The aim of the study was to evaluate the possible relationships between stress tolerance, training load, banal infections and salivary parameters during 4 weeks of regular training in fifteen basketball players. The Daily Analysis of Life Demands for Athletes' questionnaire (sources and symptoms of stress) and the Wisconsin Upper Respiratory Symptom Survey were used on a weekly basis. Salivary cortisol and salivary immunoglobulin A (SIgA) were collected at the beginning (before) and after ...
Tópico(s): Fibromyalgia and Chronic Fatigue Syndrome Research
2010 - Wiley | Stress and Health
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Ynara Bosco de Oliveira Lima‐Arsati, Émerson Franchini, Vera Cavalcanti de Araújo,
The hypothesis that salivary cortisol would increase and salivary immunoglobulin A (IgA) decrease after a kickboxing match was tested among 20 male athletes. Saliva samples collected before and after the match were analyzed. Salivary cortisol and salivary IgA concentrations (absolute concentration, salivary IgAabs) and the secretion rate of IgA (salivary IgArate) were measured by enzyme-linked immunosorbent assay. A Wilcoxon test for paired samples showed significant increases in salivary Cortisol ...
Tópico(s): Fibromyalgia and Chronic Fatigue Syndrome Research
2010 - SAGE Publishing | Perceptual and Motor Skills
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Ynara Bosco de Oliveira Lima‐Arsati, Danilo Augusto da Silva, Vera Cavalcanti de Araújo,
Tópico(s): Winter Sports Injuries and Performance
2009 - Springer Science+Business Media | European Journal of Applied Physiology
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Patrícia Ramos Cury, Clóvis Franciscon, Paulo Roberto de Oliveira, Vera Cavalcanti de Araújo,
Moreira, A, Arsati, F, Cury, PR, Franciscon, C, Oliveira, PR, and Araújo, VC. Salivary immunoglobulin a response to a match in top-level brazilian soccer players. J Strength Cond Res 23(7): 1968-1973, 2009-It has been suggested that several parameters of mucosal immunity, including salivary immunoglobulin A (s-IgA), are affected by heavy exercise either in field sports or in the laboratory environment. Few observations have been made during a true sporting environment, particularly in professional ...
Tópico(s): Sports Performance and Training
2009 - Lippincott Williams & Wilkins | The Journal of Strength and Conditioning Research
Produção Nacional
Revisado por pares
Alexandre Moreira, Franco Arsati, Patrícia Ramos Cury, Clóvis Franciscon, Antônio Carlos Simões, Paulo Roberto de Oliveira, Vera Cavalcanti de Araújo,
This investigation examined the impact of a 17-d training period (that included basketball-specific training, sprints, intermittent running exercises, and weight training, prior to an international championship competition) on salivary immunoglobulin A (SIgA) levels in 10 subjects (athletes and staff members) from a national basketball team, as a biomarker for mucosal immune defence. Unstimulated saliva samples were collected at rest at the beginning of the preparation for the Pan American Games and ...
Tópico(s): Winter Sports Injuries and Performance
2008 - Wiley | European Journal Of Oral Sciences