Vittoria Marini, Fabiola Marino, Flaminia Aliberti, Nefele Giarratana, Enrico Pozzo, Robin Duelen, Álvaro Cortés Calabuig, Rita La Rovere, Tim Vervliet, Daniele Torella, Geert Bultynck, Maurilio Sampaolesi, Yoke Chin Chai,
Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disease which to date is incurable. The major cause of death is dilated cardiomyopathy however, its pathogenesis is unclear as existing cellular and animal models do not fully recapitulate the human disease phenotypes. In this study, we generated cardiac organoids from patient-derived induced pluripotent stem cells (DMD-COs) and isogenic-corrected controls (DMD-Iso-COs) and studied if DMD-related cardiomyopathy and disease progression occur ...
Tópico(s): CRISPR and Genetic Engineering
2022 - Frontiers Media | Frontiers in Cell and Developmental Biology
Flavio Ronzoni, Nefele Giarratana, Stefania Crippa, Mattia Quattrocelli, Marco Cassano, Gabriele Ceccarelli, Laura Benedetti, Jens Van Herck, Maria Gabriella Cusella De Angelis, Marco Vitale, Daniela Galli, Maurilio Sampaolesi,
Muscular regeneration is a complex biological process that occurs during acute injury and chronic degeneration, implicating several cell types. One of the earliest events of muscle regeneration is the inflammatory response, followed by the activation and differentiation of muscle progenitor cells. However, the process of novel neuromuscular junction formation during muscle regeneration is still largely unexplored. Here, we identify by single-cell RNA sequencing and isolate a subset of vessel-associated ...
Tópico(s): Extracellular vesicles in disease
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Natacha Breuls, Nefele Giarratana, Laura Yedigaryan, Gabriel Miró Garrido, Paolo Carai, Stéphane Heymans, Adrian Ranga, Christophe M. Deroose, Maurilio Sampaolesi,
Muscular dystrophies are debilitating neuromuscular disorders for which no cure exists. As this disorder affects both cardiac and skeletal muscle, patients would benefit from a cellular therapy that can simultaneously regenerate both tissues. The current protocol to derive bipotent mesodermal progenitors which can differentiate into cardiac and skeletal muscle relies on the spontaneous formation of embryoid bodies, thereby hampering further clinical translation. Additionally, as skeletal muscle ...
Tópico(s): Muscle Physiology and Disorders
2021 - Springer Nature | Cell Death and Disease
J. Camps, Natacha Breuls, Alejandro Sifrim, Nefele Giarratana, Marlies Corvelyn, Laura Danti, Hanne Grosemans, Sebastiaan Vanuytven, Irina Thiry, M. Belicchi, Mirella Meregalli, Khrystyna Platko, Melissa E. MacDonald, Richard C. Austin, Rik Gijsbers, Giulio Cossu, Yvan Torrente, Thierry Voet, Maurilio Sampaolesi,
Fibrosis and fat replacement in skeletal muscle are major complications that lead to a loss of mobility in chronic muscle disorders, such as muscular dystrophy. However, the in vivo properties of adipogenic stem and precursor cells remain unclear, mainly due to the high cell heterogeneity in skeletal muscles. Here, we use single-cell RNA sequencing to decomplexify interstitial cell populations in healthy and dystrophic skeletal muscles. We identify an interstitial CD142-positive cell population ...
Tópico(s): Single-cell and spatial transcriptomics
2020 - Cell Press | Cell Reports
Nefele Giarratana, Filippo Conti, Rita La Rovere, Rik Gijsbers, Paolo Carai, Robin Duelen, Tim Vervliet, Geert Bultynck, Flavio Ronzoni, Roberto Piciotti, Domiziana Costamagna, Stefania Fulle, Ivana Barravecchia, Debora Angeloni, Yvan Torrente, Maurilio Sampaolesi,
Abstract Contractile myofiber units are mainly composed of thick myosin and thin actin (F-actin) filaments. F-Actin interacts with Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 2 (MICAL2). Indeed, MICAL2 modifies actin subunits and promotes actin filament turnover by severing them and preventing repolymerization. In this study, we found that MICAL2 increases during myogenic differentiation of adult and pluripotent stem cells (PSCs) towards skeletal, smooth and cardiac muscle ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2020 - Springer Nature | Cell Death and Disease
Filippo Giannetti, R. Reggiannini, Marco Moretti, Elisa Adirosi, Luca Baldini, Luca Facheris, Andrea Antonini, Samantha Melani, Giacomo Bacci, Antonio Petrolino, Attilio Vaccaro,
... the NEFOCAST project (named by the contraction of “Nefele”, which is the Italian spelling for the mythological ...
Tópico(s): Soil Moisture and Remote Sensing
2017 - Multidisciplinary Digital Publishing Institute | Sensors
Jan Toralf Fosen, Luca Morini, Cristina Sempio, Nefele Giarratana, Asle Enger, Jørg Mørland, Gudrun Høiseth,
Measurement of ethyl glucuronide (EtG) in nail, as a biomarker for alcohol intake, has recently been suggested as alternative to measurement in hair. The aim of this study was to compare levels of EtG in nail and hair, and to investigate the elimination kinetics of EtG in fingernails during an alcohol abstinent period. Overall, 40 subjects (median estimated daily intake of ethanol (EDI) 92.5 g/day) were recruited from an alcohol rehabilitation clinic. Nail and hair samples were collected at inclusion ...
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2017 - Oxford University Press | Alcohol and Alcoholism
Gabriele Ceccarelli, Rossella Presta, Saturnino Marco Lupi, Nefele Giarratana, Nora Bloise, Laura Benedetti, Maria Gabriella Cusella De Angelis, Ruggero Rodriguez y Baena,
Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in ...
Tópico(s): Bone Tissue Engineering Materials
2017 - Multidisciplinary Digital Publishing Institute | Molecules
Anna Parola, Raffaele Di Fuccio, Federica Somma, Orazio Miglino,
... It concludes with a brief description of the NEFELE Erasmus+ project that will implement tangible user interfaces ( ...
Tópico(s): Literacy, Media, and Education
2022 - Springer Science+Business Media | Communications in computer and information science
Enrico Pozzo, Nefele Giarratana, Gabriele Sassi, Merve Elmastas, Theo Killian, Chao-chi Wang, Vittoria Marini, Flavio Ronzoni, Jason T. Yustein, Anne Uyttebroeck, Maurilio Sampaolesi,
Fusion-negative rhabdomyosarcoma (FN-RMS) is the most common soft tissue sarcoma of childhood arising from undifferentiated skeletal muscle cells from uncertain origin. Currently used therapies are poorly tumor-specific and fail to tackle the molecular machinery underlying the tumorigenicity and uncontrolled proliferation of FN-RMS. We and other groups recently found that microRNAs (miRNA) network contributes to myogenic epigenetic memory and can influence pluripotent stem cell commitments. Here, ...
Tópico(s): Cancer-related molecular mechanisms research
2021 - Frontiers Media | Frontiers in Physiology
Jan Toralf Fosen, Gudrun Høiseth, Cristina Sempio, Nefele Giarratana, Asle Enger, Jørg Mørland, Luca Morini,
Abstract Hair levels of the direct ethanol biomarker ethyl glucuronide (EtG) are used to evaluate history of alcohol intake. The proximal 3 cm of the hair sample is most often analyzed and this is assumed to represent the intake of ethanol over approximately the past three months. The aim of this study was to investigate change of EtG levels during hair growth in an ethanol‐abstinent period. Twenty‐seven patients were recruited from an alcohol treatment clinic. A hair sample was collected after hospitalization ...
Tópico(s): Botulinum Toxin and Related Neurological Disorders
2018 - Wiley | Drug Testing and Analysis
Anna Parola, Federico Diano, Michela Ponticorvo, Luigia Simona Sica,
... to be trained for this role, and the NEFELE project aims to fill this gap. The training ...
Tópico(s): Identity, Memory, and Therapy
2024 - Frontiers Media | Frontiers in Psychology
Laura Yedigaryan, Ester Martínez‐Sarrà, Giorgia Giacomazzi, Nefele Giarratana, Bernard K. van der Veer, Alessio Rotini, Silvia Querceto, Hanne Grosemans, Álvaro Cortés-Calabuig, Sara Salucci, Michela Battistelli, Elisabetta Falcieri, Rik Gijsbers, Mattia Quattrocelli, Kian Peng Koh, Liesbeth De Waele, Gunnar Buyse, Rita Derua, Maurilio Sampaolesi,
Skeletal muscle holds an intrinsic capability of growth and regeneration both in physiological conditions and in case of injury. Chronic muscle illnesses, generally caused by genetic and acquired factors, lead to deconditioning of the skeletal muscle structure and function, and are associated with a significant loss in muscle mass. At the same time, progressive muscle wasting is a hallmark of aging. Given the paracrine properties of myogenic stem cells, extracellular vesicle-derived signals have ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2022 - Frontiers Media | Frontiers in Immunology
Anna Parola, Federico Diano, Luigia Simona Sica,
Tópico(s): Technology Assessment and Management
2024 - Springer International Publishing | Lecture notes in networks and systems
Francisco Javier Villegas-Soto, Mijael Altamirano Santiago, Adalberto de Hoyos,
... de sometimiento, utilizando la metáfora de Ixión y Néfele, la cual describe adecuadamente el comportamiento de la ...
Tópico(s): Law, Ethics, and AI Impact
2022 - | Revista de Investigaciones Universidad del Quindío
Enrico Pozzo, Laura Yedigaryan, Nefele Giarratana, Chao-chi Wang, Gabriel Miró Garrido, Ewoud Degreef, Vittoria Marini, Gianmarco Rinaldi, Bernard K. van der Veer, Gabriele Sassi, Guy Eelen, Mélanie Planque, Alessandro Fanzani, Kian Peng Koh, Peter Carmeliet, Jason T. Yustein, Sarah‐Maria Fendt, Anne Uyttebroeck, Maurilio Sampaolesi,
Rhabdomyosarcoma (RMS), the most common pediatric soft tissue sarcoma, arises in skeletal muscle and remains in an undifferentiated state due to transcriptional and post-transcriptional regulators. Among its subtypes, fusion-negative RMS (FN-RMS) accounts for the majority of diagnoses in the pediatric population. MicroRNAs (miRNAs) are non-coding RNAs that modulate cell identity via post-transcriptional regulation of messenger RNAs (mRNAs). In this study, we identify miRNAs impacting FN-RMS cell identity, ...
Tópico(s): MicroRNA in disease regulation
2025 - Cell Press | Cell Reports
Nefele Giarratana, Filippo Conti, Lorenza Rinvenuti, Flavio Ronzoni, Maurilio Sampaolesi,
Adult skeletal muscle is a dynamic tissue able to regenerate quite efficiently, thanks to the presence of stem cell machinery. Besides the quiescent satellite cells that are activated upon injury or paracrine factors, other stem cells are described to be directly or indirectly involved in adult myogenesis. Mesoangioblasts (MABs) are vessel-associated stem cells originally isolated from embryonic dorsal aorta and, at later stages, from the adult muscle interstitium expressing pericyte markers. Adult ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2023 - Springer Science+Business Media | Methods in molecular biology
Davide Gabellini, Antonio Musarò, M Linari, N Elvassore, J Ruas, Mark A. Tarnopolsky, K Bernadzki, M Gawor, Piotr Niewiadomski, B.M. Pradhan, Tomasz Prószyński, A Franco-Romero, Gargulak Milan, Romanello, Renata Sartori, M. Sandri, Giovanni Gherardi, De Stefani, Rosario Rizzuto, C Mammucari, Matteo Giovarelli, Silvia Zecchini, M Brucoli, C. Palma, Emily O’Connor, Phan, I Cordts, G Cairns, Albert de Roos, Hanns Lochmüller, A F Isham, Carol L. Bowers, O ́Connor E, Denisa Hathazi, Ana Maria Jimenez-Moreno, Oksana Pogoryelova, D Furling, I Verhaart, N Nikolenko, Michael Saclier, George Bedinelli Rossi, S Antonini, M Lapi, Corrado Bonfanti, Giovanni Messina, Taglietti, S Monteverde, F Guarnier, L Pietrangelo, M Serano, S Boncompagni, F Protasi, C Pecorai, Aaron Michelucci, Adriano Fonzino, Elaine Conte, G Camerino, A Liantonio, M Bellis, Andrea Mele, D Tricarico, Gabriella Dobrowolny, S Pierno, Federica Maiole, Fabio Benfenati, Letizia Zullo, M Bertin, N Marroncelli, M Bianchi, S Consalvi, Saccone, M Bardi, Poonam Puri, Douchan Palacios, Sara Adamo, Moresi, Ilaria Di Renzo, E. Clementi, C Perrotta, Ersilia Fornetti, Simone Testa, Claudia Fuoco, G Cesareni, S Cannata, C Gargioli, Mariangela Marrone, R Rovere, Simone Guarnieri, Simone Fulle, Geert Bultynck, Rocco L. Mancinelli, Frédéric Relaix, A Urciuolo, E Serena, L Urbani, S Perin, Libero Vitiello, B Blaauw, Paolo De Coppi, N Elavassore, Eva Galletta, F Majo, L Tibaudo, Giorgia Giacomazzi, L Madaro, Serena Mandla, P. Gilbert, Lynn Smith, Kok H, EN Barton, Bianconi, Beatrice Biferali, C Mozzetta, Steve Carr, M Ritso, Theodor von Brand, Steffen Krebs, Alexander Graf, Harold F. Blum, Karl G. Lorenz, Hanns Lochmüller, A Pasut, M Manilla, Eusebio Perdiguero, Pura Muñoz‐Cánoves, F. Rossi, Sartorelli, C Provenzano, M Cappella, R Valaperta, R Cardani, G. Meola, Francesca K. A. Martelli, B Cardinali, Guido J. Falcone, E Pigna, Emanuele Greco, E Simonazzi, Al Rickard, J Arjomand, Alex S. Kiselyov, Ute Schmidt, M Soardi, M Carotti, C Fecchio, Roberta Sacchetto, D Sandonà, Evelyn Marie Caruso, K Petroni, C Tonelli, Francesca Solagna, Leonardo Nogara, K Dyar, F Chemello, H Uhlenhaut, K Vissing, Markus Krüger, Milica Marinkovic, Lucia Lisa Petrilli, F Spada, Francesca Sacco, Mônica Steffen Guise Rosina, Melanie Mann, Luisa Castagnoli, D Owens, J Mésséant, G Herledan, Antoine Ferry, A. R. Bertrand, Gisèle Bonne, C Coirault, G Belotti, Graziano Ceccarelli, L Benedetti, Fernando Mulas, Riccardo Bellazzi, Michela De Angelis, Maurilio Sampaolesi, F Ronzoni, Denis Vecellio Reane, F Vallese, Vanessa Checchetto, Laura Acquasaliente, Gisela Butera, Filippis, István Szabó, Gloria Zanotti, A Raffaello, Natacha Breuls, Domiziana Costamagna, Bryan Holvoet, R Duelen, Sahakyan, Gobbo, E Germinario, E Martínez-Sarrà, S Montori, C Gil-Recio, R Núñez-Toldrà, A Rotini, M Atari, A Luttun, Sally Spendiff, RJ Howarth, G McMacken, Silvia Cipriani, Rita Horváth, Paola Frattini, Carina Magali Yaucán Villa, Frank J. De Santis, M Meregalli, M Belicchi, S Erratico, Professor Hassan Bella, Manuela Teresa Raimondi, Lu Q, Y Torrente, S Codenotti, M. Poli, M Asperti, A Fanzani, Laura Pasetto, Davide Olivari, Giovanni Nardo, Caterina Bendotti, R Piccirillo, Bonetto, A Cecconi, G Martinelli, S Previdi, Sergio Marchini, Ludovica Beltrame, E Filippo, G Nefele, H Sustova, M Feudis, S Reano, F Prodam, N Filigheddu, Elena Agosti, S Clerici, M Ferrara, E Angelino, Alberto Graziani, Giosuè Annibalini, Mauro De Santi, S Contarelli, R Saltarelli, M Guescini, Luciana Vallorani, Giorgio Brandi, Stocchi, Edoardo Roberto Barbieri, R Lakshmi, N Bursac, Flavio Ascenzi, Laura Barberi, C Nicoletti, Marián Navarro Beltrá, F. Pin, R Ballarò, Amanda Iannuzzi, Francesca Della Penna, P Costelli, F Blegniski, R Cecchini, F Bonazza, Stephen M. Gavazzi, Luca Brocca, Roberto Bottinelli, Maurizio Pellegrino, D Burini, S Salucci, M Battistelli, S Burattini, P Gobbi, Elisabetta Falcieri, D Curzi, P Ceccaroli, S Maggio, E Polidori, F Lucertini, Sara Chiappalupi, F Riuzzi, L Salvadori, R Sagheddu, Roberto Di Donato, Guglielmo Sorci, M. Gervasi, Candela Marini, F Fardetti, E. Grassi, P Benelli, de Winter J, Jaap Molenaar, Manuela Marabita,
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