Shu-Ping Lu, Michiko Kato, Su-Ju Lin,
... are mainly mediated by a conserved NmR kinase (Nrk1) and three nucleosidases (Urh1, Pnp1, and Meu1). Nrk1 phosphorylates NmR and NaR to produce nicotinamide mononucleotide ( ... marker using a galactose inducible Cre-recombinase. The Nrk1 overexpression construct was made as follows: a pair ... NheI site to the 3′ end of the NRK1 gene was designed to amplify the coding region of NRK1 via PCR. After PCR amplification, DNA was digested ... PubMed Scopus (50) Google Scholar), resulting in pADH1-NRK1, which was verified by DNA sequencing. Anaerobic growth ...
Tópico(s): Tryptophan and brain disorders
2009 - Elsevier BV | Journal of Biological Chemistry
Michiko Sasabe, Takashi Soyano, Yuji Takahashi, Seiji Sonobe, Hisako Igarashi, Tomohiko J. Itoh, Mikiko Hidaka, Yasunori Machida,
... activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery ... expansion were not known. Here, we show that NRK1/NTF6 phosphorylates the threonine residue at position 579 ... late M phase of the cell cycle, when NRK1/NTF6 is activated. Phosphorylated NtMAP65-1 is concentrated at the equator of phragmoplast, as is NRK1/NTF6. Overexpression of mutant forms of NtMAP65-1a that cannot be phosphorylated by NRK1 delays progression of the M phase and phragmoplast ...
Tópico(s): Photosynthetic Processes and Mechanisms
2006 - Cold Spring Harbor Laboratory Press | Genes & Development
Yasushi Kitaoka, Kana Sase, Chihiro Tsukahara, Naoki Fujita, Ibuki Arizono, Hitoshi Takagi,
... examined the localization of nicotinamide riboside kinase 1 (NRK1), which is a downstream enzyme for NR biosynthesis ... optic nerve. Immunohistochemical analysis was performed to localize NRK1 expression. Morphometric analysis showed substantial axonal protection by ... levels in optic nerve. Immunohistochemical analysis showed that NRK1 exists in retinal ganglion cells (RGCs) and nerve ... retina and optic nerve. NR administration apparently upregulated NRK1 levels in the TNF-treated eyes as well ... implicating that SIRT1 regulates autophagy status. In conclusion, NRK1 exists in RGCs and optic nerve axons. NR ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2020 - Springer Science+Business Media | Molecular Neurobiology
Rui Fan, Jing Cui, Feng Ren, Qingzhi Wang, Yanmei Huang, Bin Zhao, Lai Wei, Xinlai Qian, Xiwen Xiong,
... identify the roles of nicotinamide riboside kinase 1 (NRK1) plays in regulating hepatic NAD+ biosynthesis and lipid ... adenovirus mediated gene transduction to overexpress or knockdown NRK1 in mouse liver, we have demonstrated that NRK1 is critical for maintaining hepatic NAD+ levels and ... or in aged mice. However, adenoviral delivery of NRK1 in these diet- and age-induced mice elevates ... sensitivity. Our results provide important insights in targeting NRK1 for treating hepatic steatosis.
Tópico(s): Autophagy in Disease and Therapy
2018 - Elsevier BV | Biochemical and Biophysical Research Communications
Audrey Sambeat, Joanna Ratajczak, Magali Joffraud, José Luis Sánchez, Maria Pilar Giner, Armand Valsesia, Judith Giroud‐Gerbetant, Miriam Valera‐Alberni, Angelique Cercillieux, Marie Boutant, Sameer S. Kulkarni, Sofia Moco, Carles Cantó,
... We have previously shown that NR kinase 1 (NRK1) is rate-limiting and essential for NR-induced ... metabolism, we generated whole body and liver-specific NRK1 knockout mice. Here, we show that NRK1 deficiency leads to decreased gluconeogenic potential and impaired mitochondrial function. Upon high-fat feeding, NRK1 deficient mice develop glucose intolerance, insulin resistance and ... diet-induced metabolic damage, highlighting the relevance of NRK1 as a therapeutic target for metabolic disorders.
Tópico(s): PARP inhibition in cancer therapy
2019 - Nature Portfolio | Nature Communications
Rachel S. Fletcher, Joanna Ratajczak, Craig Doig, Lucy Oakey, Rebecca Callingham, Gabriela da Silva Xavier, Antje Garten, Yasir Elhassan, Philip Redpath, Marie E. Migaud, Andrew Philp, Charles Brenner, Carles Cantó, Gareth G. Lavery,
... single and double nicotinamide riboside kinase 1/2 (NRK1/2) loss-of-function mice, and pharmacological inhibition ... muscle cells primarily rely on nicotinamide phosphoribosyltransferase (NAMPT), NRK1, and NRK2 for salvage biosynthesis of NAD+. NAMPT ... cell NAD+ in a pathway that depends on NRK1 and NRK2. Nrk2 knockout mice develop normally and ... their NAD+ metabolome and expression of related genes. NRK1, NRK2, and double KO myotubes revealed redundancy in ... NAMPT to maintain NAD+ availability and reveal that NRK1 and 2 display overlapping function in salvage of ...
Tópico(s): Calcium signaling and nucleotide metabolism
2017 - Elsevier BV | Molecular Metabolism
Hanna Gräns, Peter Nilsson, Birgitta Evengård,
... three of the five genes (CD83, BOLA1 and NRK1) (Fig. 1) with real-time PCR. Significance was ... both normalization genes, 18S rRNA and GAPDH. CD83, NRK1 and BOLA1 were identified with significant differential gene ... activity level in patients compared with controls, whilst NRK1 and BOLA1 are overexpressed. The five genes differentiated ... been used as medical treatment for CFS patients. NRK1 is an enzyme involved in the synthesis of ... and gradual illness onset) compared with healthy controls. NRK1 and BOLA1 are overexpressed in the patient group. ...
Tópico(s): Health, psychology, and well-being
2005 - Wiley | Journal of Internal Medicine
Sofie Lautrup, David Sinclair, Mark P. Mattson, Evandro Fei Fang,
... NR into NMN by nicotinamide riboside kinase 1 (NRK1) or NRK2 (Bieganowski and Brenner, 2004Bieganowski P. Brenner ... M. Redpath P. Migaud M.E. et al.NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in ... M. Redpath P. Migaud M.E. et al.NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in ... M. Redpath P. Migaud M.E. et al.NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in ...
Tópico(s): Calcium signaling and nucleotide metabolism
2019 - Cell Press | Cell Metabolism
... to nicotinamide mononucleotide (NMN) by the NR kinase Nrk1 (Figure 1). NMN is then readily converted to ... Scholar). Utilization of NR requires either an active Nrk1 or an active Urh1/Pnp1 pathway. In the absence of NR supplementation, the Nrk1 and Urh1/Pnp1 pathways contribute to NAD+ levels, ... to nicotinamide mononucleotide by specific nicotinamide riboside kinases, Nrk1 and Nrk2. In this study, we discovered that ...
Tópico(s): Tryptophan and brain disorders
2007 - Cell Press | Cell
Takashi Soyano, Ryuichi Nishihama, Kayoko Morikiyo, Masaki Ishikawa, Yasunori Machida,
... study, we have identified tobacco NQK1/NtMEK1 and NRK1 as a MAPKK and a MAPK, respectively, downstream ... NQK1/NtMEK1 phosphorylate and activate NQK1/NtMEK1 and NRK1, respectively. Both NQK1/NtMEK1 and NRK1, as well as NPK1, are activated at the ... cascade that consists of NPK1, NQK1/NtMEK1, and NRK1 and functions in a process related to the ...
Tópico(s): Plant-Microbe Interactions and Immunity
2003 - Cold Spring Harbor Laboratory Press | Genes & Development
Joanna Ratajczak, Magali Joffraud, Samuel A.J. Trammell, Rosa Ras, Núria Canela-Canela, Marie Boutant, Sameer S. Kulkarni, Marcelo Rodrigues Rodrigues, Philip Redpath, Marie E. Migaud, Johan Auwerx, Óscar Yanes, Charles Brenner, Carles Cantó,
... Here we show that nicotinamide riboside kinase 1 (NRK1) is necessary and rate-limiting for the use ... models, we further demonstrate that the role of NRK1 in driving NAD + synthesis from other NAD + precursors, ...
Tópico(s): PARP inhibition in cancer therapy
2016 - Nature Portfolio | Nature Communications
Dahyun Hwang, HyunA Jo, Seong-Ho Ma, Young‐Hee Lim,
... of NAD+-synthesis enzymes, NaPRT1, nicotinamide riboside kinase1 (NRK1) and nicotinamide mononucleotide adenylyltransferase1 (Nmnat1) was significantly increased ... LS 174T cells. The inhibition of NaPRT1 and NRK1 did not decrease MUC2 expression after inhibiting by small interfering RNA (siRNA)-NaPRT1 and siRNA-NRK1, respectively; however, inhibition of Nmnat by an Nmnat ...
Tópico(s): Cytomegalovirus and herpesvirus research
2018 - Elsevier BV | Food and Chemical Toxicology
Sakari Jukarainen, Sini Heinonen, Joel Rämö, Rita Rinnankoski‐Tuikka, Elisabeth Rappou, Mark Tümmers, Maheswary Muniandy, Antti Hakkarainen, Jesper Lundbom, Nina Lundbom, Jaakko Kaprio, Aila Rissanen, Eija Pirinen, Kirsi H. Pietiläinen,
... test.SIRT1, SIRT3, SIRT5, NAMPT, NMNAT2, NMNAT3, and NRK1 expressions were significantly down-regulated and the activity ... twin-shared factors, SIRT1, SIRT3, NAMPT, NMNAT3, and NRK1 were significantly negatively correlated with adiposity, SIRT1, SIRT5, NMNAT2, NMNAT3, and NRK1 were negatively correlated with inflammation, and SIRT1 and ...
Tópico(s): Tea Polyphenols and Effects
2015 - Oxford University Press | The Journal of Clinical Endocrinology & Metabolism
W. Tempel, Wael M. Rabeh, Katrina L. Bogan, Peter Belenky, Marzena Wójcik, Heather F. Seidle, L. Nedyalkova, Tianle Yang, Anthony A. Sauve, Hee-Won Park, Charles Brenner,
... by phosphorylation and adenylylation. Crystal structures of human Nrk1 bound to nucleoside and nucleotide substrates and products ... which were shown to be essential for human Nrk1 and Nrk2 activity in vivo. Although the structures ... and Preiss-Handler salvage. Thus, crystal structures of Nrk1 led to the identification of new pathways to ...
Tópico(s): PARP inhibition in cancer therapy
2007 - Public Library of Science | PLoS Biology
Peter Belenky, Frances G. Racette, Katrina L. Bogan, Julie M. McClure, Jeffrey S. Smith, Charles Brenner,
... to nicotinamide mononucleotide by specific nicotinamide riboside kinases, Nrk1 and Nrk2. In this study, we discovered that ... increased net NAD(+) synthesis through two pathways, the Nrk1 pathway and the Urh1/Pnp1/Meu1 pathway, which is Nrk1 independent. Additionally, the two nicotinamide riboside salvage pathways ...
Tópico(s): Adipose Tissue and Metabolism
2007 - Cell Press | Cell
Xiaojing Wei, Chuanfei Wei, Yutian Tan, Ximing Dong, Zhao Yang, Jianqun Yan, Xiao Luo,
... the enzymes involved in NAD+ salvage (Nampt, Nmnat1, Nrk1), clearance (Nnmt, Aox1, Cyp2e1), and consumption pathways (Sirt1, ... concomitant with positive correlations with Pck1 gene expression. Nrk1 and Cyp2e1 gene expression positively correlated with fat ...
Tópico(s): Autophagy in Disease and Therapy
2023 - Elsevier BV | The Journal of Nutritional Biochemistry
Joanna A. Ruszkiewicz, Alexander Bürkle, Aswin Mangerich,
... Ras R. Canela N. Boutant M. et al.NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in ... Ras R. Canela N. Boutant M. et al.NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in ...
Tópico(s): Biochemical and Molecular Research
2022 - Elsevier BV | Journal of Biological Chemistry
Thomas J. Waller, Catherine A. Collins,
... NR) (while overexpressing the NMN-synthesizing NR kinase [NRK1]). While this popular nutritional supplement is used for ... explain why neurons pretreated with NR together with NRK1 show an increased resilience to degeneration (Figley et ...
Tópico(s): Adenosine and Purinergic Signaling
2021 - Cell Press | Neuron
Xiaojing Wei, Ru Jia, Guan Wang, Shangyu Hong, Lin Song, Bo Sun, Ke Chen, Nan Wang, Qiqi Wang, Xiao Luo, Jianqun Yan,
... on key enzymes in NAD+ salvage (Nampt, Nmnat1, Nrk1), clearance (Nnmt, Aox1, Cyp2e1) and consumption pathways (Sirt1, ... energy imbalance in WAT but not in BAT. Nrk1 gene expression was decreased in eWAT and iWAT ...
Tópico(s): Tea Polyphenols and Effects
2020 - Elsevier BV | The Journal of Nutritional Biochemistry
Yue Yang, Naseer Ahmed Khan Mohammed, Ning Zhang, Anthony A. Sauve,
... NRH to the compound NMNH, but independent of Nrk1 or Nrk2. These studies identify a putative new ... NRH to the compound NMNH, but independent of Nrk1 or Nrk2. These studies identify a putative new ...
Tópico(s): Tryptophan and brain disorders
2019 - Elsevier BV | Journal of Biological Chemistry
Peter Belenky, Tiberiu Moga, Charles Brenner,
... demonstrated in 2004, depends on eukaryotic NR kinase Nrk1 or Nrk2 (2Bieganowski P. Brenner C. Cell. 2004; ... precursor converted to NAD+ in pathways initiated by Nrk1, Urh1, and Pnp1 (12Tempel W. Rabeh W.M. ...
Tópico(s): Fungal and yeast genetics research
2008 - Elsevier BV | Journal of Biological Chemistry
Tanja Sonntag, Sara Ancel, Sonia Karaz, Paulina Cichosz, Guillaume Jacot, Maria Pilar Giner, José Luis Sánchez, Alice Pannérec, Sofia Moco, Vincenzo Sorrentino, Carles Cantó, Jérôme N. Feige,
... plasticity during skeletal muscle growth and remodeling. Using NRK1/2 double KO (NRKdKO) mice, we investigated the ... controls mitochondrial metabolism and fiber type composition via NRK1/2 and is rate-limiting for myogenic commitment ...
Tópico(s): Adipose Tissue and Metabolism
2022 - Frontiers Media | Frontiers in Cell and Developmental Biology
Jian-Ju He, Xinxin Liu, Ying Li, Zhe Wang, Hongling Shi, Yunchao Kan, Lunguang Yao, Cun‐Duo Tang,
... gene fragments from S. cerevisiae , and the Sc NRK1 was achieved a high level of soluble expression ...
Tópico(s): Calcium signaling and nucleotide metabolism
2023 - Frontiers Media | Frontiers in Bioengineering and Biotechnology
Chongkui Sun, Kun Wang, Amanda J. Stock, Yi Gong, Tyler G. Demarest, Beimeng Yang, Neelam Giri, Lea Harrington, Blanche P. Alter, Sharon A. Savage, Vilhelm A. Bohr, Yie Liu,
... precursor, NR. NR is converted to NMN by NRK1/2 synthesizing enzymes, followed by conversion of NMN ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2020 - Springer Nature | The EMBO Journal
Jerre M. Madern, Robbert Q. Kim, Mohit Misra, Ivan Đikić, Yong Zhang, Huib Ovaa, Jeroen D. C. Codée, Dmitri V. Filippov, Gerbrand J. van der Heden van Noort,
... expanded the known scope of substrates for the NRK1/NMNAT1 enzyme combination by turning all three modified ...
Tópico(s): Autophagy in Disease and Therapy
2020 - Wiley | ChemBioChem
Judith Giroud‐Gerbetant, Magali Joffraud, Maria Pilar Giner, Angelique Cercillieux, Simona Bártová, Mikhail V. Makarov, Rubén Zapata‐Pérez, José Luis Sánchez, Riekelt H. Houtkooper, Marie E. Migaud, Sofia Moco, Carles Cantó,
... enzymes to synthesize NAD+, thus revealing a new NRK1-independent pathway for NAD+ synthesis. Finally, we provide ...
Tópico(s): Cancer-related cognitive impairment studies
2019 - Elsevier BV | Molecular Metabolism
Roldan M. de Guia, Marianne Agerholm, Thomas S. Nielsen, Leslie A. Consitt, Ditte Søgaard, Jørn Wulff Helge, Steen Larsen, Josef Brandauer, Joseph A. Houmard, Jonas T. Treebak,
... of young and old people, levels of NAMPT, NRK1, and NMNAT1/2 in abdominal subcutaneous adipose tissue ...
Tópico(s): Muscle Physiology and Disorders
2019 - Wiley | Physiological Reports
David C. Schöndorf, Dina Ivanyuk, Pascale Baden, Álvaro Sánchez-Martínez, Silvia De Cicco, Cong Yu, Ivana Giunta, Lukas Schwarz, Gabriele Di Napoli, Vasiliki Panagiotakopoulou, Sigrun Nestel, Marcus Keatinge, Jan Pruszak, Oliver Bandmann, Bernd Heimrich, Thomas Gasser, Alexander J. Whitworth, Michela Deleidi,
... NAMPT) to maintain the NAD+ pool and utilize NRK1 to synthesize NAD+ from NAD+ precursors. Remarkably, NR ...
Tópico(s): Autophagy in Disease and Therapy
2018 - Cell Press | Cell Reports
Giovanna Sociali, Lizzia Raffaghello, Mirko Magnone, Federica Zamporlini, Laura Emionite, Laura Sturla, Giovanna Bianchi, Tiziana Vigliarolo, Aimable Nahimana, Alessio Nencioni, Nadia Raffaelli, Santina Bruzzone,
... line (OVCAR-3), by means of CD73 or NRK1 specific silencing. Next, we investigated the anti-tumor ...
Tópico(s): PARP inhibition in cancer therapy
2015 - Impact Journals LLC | Oncotarget
... second pathway involves kinases called nicotinamide riboside kinases (Nrk1 and Nrk2, in humans). The likely involvement of ...
Tópico(s): Coconut Research and Applications
2013 - Lippincott Williams & Wilkins | Current Opinion in Clinical Nutrition & Metabolic Care