Kurt Wollenberg, Michael A. Harris, Andrei Gabrielian, Nelly Ciobanu, Dumitru Chesov, Alyssa Long, Jessica Taaffe, Darrell E. Hurt, Alex Rosenthal, Michael Tartakovsky, Valeriu Crudu,
Recurrence of drug-resistant tuberculosis (DR-TB) after treatment occurs through relapse of the initial infection or reinfection by a new drug-resistant strain. Outbreaks of DR-TB in high burden regions present unique challenges in determining recurrence status for effective disease management and treatment. In the Republic of Moldova the burden of DR-TB is exceptionally high, with many cases presenting as recurrent.We performed a retrospective analysis of Mycobacterium tuberculosis from Moldova ...
Tópico(s): Infectious Diseases and Tuberculosis
2020 - BioMed Central | BMC Infectious Diseases
Bárbara Molina-Moya, Nelly Ciobanu, Marta Hernández, Cristina Prat, Valeriu Crudu, Emily R. Adams, Alexandru Codreanu, Derek J. Sloan, Luís E. Cuevas, José Domínguez,
Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, ...
Tópico(s): Mycobacterium research and diagnosis
2020 - Multidisciplinary Digital Publishing Institute | Journal of Clinical Medicine
Adam Penn‐Nicholson, Sophia B. Georghiou, Nelly Ciobanu, Mubin Kazi, Manpreet Bhalla, Anura David, Francesca Conradie, Morten Rühwald, Valeriu Crudu, Camilla Rodrigues, Vithal Prasad Myneedu, Lesley Scott, Claudia M. Denkinger, Samuel G. Schumacher,
Background The WHO End TB Strategy requires drug susceptibility testing and treatment of all people with tuberculosis, but second-line diagnostic testing with line-probe assays needs to be done in experienced laboratories with advanced infrastructure. Fewer than half of people with drug-resistant tuberculosis receive appropriate treatment. We assessed the diagnostic accuracy of the rapid Xpert MTB/XDR automated molecular assay (Cepheid, Sunnyvale, CA, USA) to overcome these limitations. Methods We ...
Tópico(s): Antibiotic Resistance in Bacteria
2021 - Elsevier BV | The Lancet Infectious Diseases
Chongguang Yang, Benjamin Sobkowiak, Vijay Naidu, Alexandru Codreanu, Nelly Ciobanu, Kenneth S. Gunasekera, Melanie H. Chitwood, Sofia Alexandru, Stela Bivol, Marcus Russi, Joshua Havumaki, Patrick Cudahy, Heather Fosburgh, Christopher J. Allender, Heather Centner, David M. Engelthaler, Nicolas A. Menzies, Joshua L. Warren, Valeriu Crudu, Caroline Colijn, Ted Cohen,
The incidence of multidrug-resistant tuberculosis (MDR-TB) remains critically high in countries of the former Soviet Union, where >20% of new cases and >50% of previously treated cases have resistance to rifampin and isoniazid. Transmission of resistant strains, as opposed to resistance selected through inadequate treatment of drug-susceptible tuberculosis (TB), is the main driver of incident MDR-TB in these countries.We conducted a prospective, genomic analysis of all culture-positive TB cases diagnosed ...
Tópico(s): Pneumonia and Respiratory Infections
2022 - Public Library of Science | PLoS Medicine
Sudhasini Panda, Jeffrey Morgan, Catherine Cheng, Mayuko Saito, Robert H. Gilman, Nelly Ciobanu, Valeriu Crudu, Donald G. Catanzaro, Antonino Catanzaro, Timothy C. Rodwell, Judy Perera, Teshan Chathuranga, Bandu Gunasena, Aruna Dharshan De Silva, Bjoern Peters, Alessandro Sette, Cecilia S. Lindestam Arlehamn,
There is still incomplete knowledge of which Mycobacterium tuberculosis (Mtb) antigens can trigger distinct T cell responses at different stages of infection. Here, a proteome-wide screen of 20,610 Mtb-derived peptides in 21 patients mid-treatment for active tuberculosis (ATB) reveals IFNγ-specific T cell responses against 137 unique epitopes. Of these, 16% are recognized by two or more participants and predominantly derived from cell wall and cell processes antigens. There is differential recognition ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
2024 - Nature Portfolio | Nature Communications
Dumitru Chesov, Sofia Alexandru, Valeriu Crudu, Nelly Ciobanu, Victor Botnaru, Jan Heyckendorf, Christoph Lange,
Tópico(s): Tuberculosis Research and Epidemiology
2019 - International Union Against Tuberculosis and Lung Disease | The International Journal of Tuberculosis and Lung Disease
Tyler S. Brown, Vegard Eldholm, Ola Brynildsrud, Magnus N Osnes, Natalie S. Levy, James Stimson, Caroline Colijn, Sofia Alexandru, Ecaterina Noroc, Nelly Ciobanu, Valeriu Crudu, Ted Cohen, Barun Mathema,
The evolution and emergence of drug-resistant tuberculosis (TB) has been studied extensively in some contexts, but the ecological drivers of these two processes remain poorly understood. This study sought to describe the joint evolutionary and epidemiological histories of a novel multidrug-resistant Mycobacterium tuberculosis strain recently identified in the capital city of the Republic of Moldova (MDR Ural/4.2), where genomic surveillance of drug-resistant M. tuberculosis has been limited thus far. ...
Tópico(s): Evolution and Genetic Dynamics
2021 - Microbiology Society | Microbial Genomics
Dumitru Chesov, Nelly Ciobanu, Christoph Lange, Jan Heyckendorf, Valeriu Crudu,
Since May 2016 the World Health Organization (WHO) has recommended the treatment of multidrug-resistant tuberculosis (MDR-TB) patients with a standardised treatment regimen of 9–12 months duration if patients fulfil specific eligibility criteria [1]. This shorter-course MDR-TB treatment regimen consists of a combination of seven drugs (clofazimine, ethambutol, high-dose isoniazid, kanamycin, moxifloxacin, prothionamide and pyrazinamide) for 4–6 months, followed by four drugs (moxifloxacin, clofazimine, ...
Tópico(s): Mycobacterium research and diagnosis
2017 - European Respiratory Society | European Respiratory Journal
Dumitru Chesov, Nelly Ciobanu, Christoph Lange, Thomas Schön, Jan Heyckendorf, Valeriu Crudu,
We are grateful for the response by E. Heldal to our correspondence. Similar to other reports [1] from the World Health Organization (WHO) Euro-Region we found that a high proportion (88%) of Mycobacterium tuberculosis strains from patients with multidrug-resistant (MDR)/extensively drug-resistant (XDR) tuberculosis (TB) in the Republic of Moldova had a katG 315T mutation, which is strongly suggestive that high-dose isoniazid should not be part of a standardised treatment regimen for patients with MDR/ ...
Tópico(s): Diagnosis and treatment of tuberculosis
2017 - European Respiratory Society | European Respiratory Journal
Leigh Ann Cates, Valeriu Crudu, Alexandru Codreanu, Nelly Ciobanu, Heather Fosburgh, Ted Cohen, Nicolas A. Menzies,
Tópico(s): Mycobacterium research and diagnosis
2021 - International Union Against Tuberculosis and Lung Disease | The International Journal of Tuberculosis and Lung Disease
Melanie H. Chitwood, Caroline Colijn, Chongguang Yang, Valeriu Crudu, Nelly Ciobanu, Alexandru Codreanu, Jaehee Kim, Isabel Rancu, Kyu Y. Rhee, Ted Cohen, Benjamin Sobkowiak,
Abstract The projected trajectory of multidrug resistant tuberculosis (MDR-TB) epidemics depends on the reproductive fitness of circulating strains of MDR M. tuberculosis (Mtb) . Previous efforts to characterize the fitness of MDR Mtb have found that Mtb strains of the Beijing sublineage (Lineage 2.2.1) may be more prone to develop resistance and retain fitness in the presence of resistance-conferring mutations than other lineages. Using Mtb genome sequences from all culture-positive cases collected ...
Tópico(s): Evolution and Genetic Dynamics
2024 - Nature Portfolio | Nature Communications
Sheng‐Wei Pan, Donald G. Catanzaro, Marva Seifert, Rehan R. Syed, Naomi Hillery, Mei‐Lin Ho, Valeriu Crudu, Elena Tudor, Nelly Ciobanu, Alexandru Codreanu, Antonino Catanzaro, Timothy C. Rodwell,
To assess associations between disease severity in index TB patients and QuantiFERON-TB Gold Plus (QFT-Plus) results in contacts, and predictors for QFT-Plus conversion in contacts over 6-12 months. TB patients (n=295) and the contacts (n=1051) were enrolled during 2018-2021 with QFT-Plus performed at baseline and months 6 and 12. A strong CD8 response was defined as TB2 interferon gamma (IFN-γ) response minus TB1 >0.6 IU/ml and stringent conversion as change from QFT-plus negative to high-positive QFT- ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2023 - Elsevier BV | Journal of Microbiology Immunology and Infection
Rehan R. Syed, Donald G. Catanzaro, Rebecca E. Colman, Christopher G. Cooney, Yvonne Linger, Alexander V. Kukhtin, Rebecca C. Holmberg, Ryan Norville, Valeriu Crudu, Nelly Ciobanu, Alexandru Codreanu, Marva Seifert, Naomi Hillery, Peter G. Chiles, Antonino Catanzaro, Timothy C. Rodwell,
While the goal of universal drug susceptibility testing has been a key component of the WHO End TB Strategy, in practice, this remains inaccessible to many. Rapid molecular tests for tuberculosis (TB) and antituberculosis drug resistance could significantly improve access to testing. In this study, we evaluated the accuracy of the Akonni Biosystems XDR-TB (extensively drug-resistant TB) TruArray and lateral-flow-cell (XDR-LFC) assay (Akonni Biosystems, Inc., Frederick, MD, USA), a novel assay that detects ...
Tópico(s): Pneumonia and Respiratory Infections
2023 - American Society for Microbiology | Journal of Clinical Microbiology
Shiying You, Melanie H. Chitwood, Kenneth S. Gunasekera, Valeriu Crudu, Alexandru Codreanu, Nelly Ciobanu, Jennifer Furin, Ted Cohen, Joshua L. Warren, Reza Yaesoubi,
Background Limited access to drug-susceptibility tests (DSTs) and delays in receiving DST results are challenges for timely and appropriate treatment of multi-drug resistant tuberculosis (TB) in many low-resource settings. We investigated whether data collected as part of routine, national TB surveillance could be used to develop predictive models to identify additional resistance to fluoroquinolones (FLQs), a critical second-line class of anti-TB agents, at the time of diagnosis with rifampin-resistant ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2022 - Public Library of Science | PLOS Digital Health
N. A. Enaki, Nelly Ciobanu, Miguel Orszag,
The influence of the standing wave amplitude on the resonance fluorescence of a three-level system of radiators is discussed. Great attention is devoted to the peculiarities of dressed states in the standing wave and the exchange integrals between two atoms situated in the anti-nodes or the nodes. The correlation functions of emitted photons at two dressed frequencies were obtained. It is shown that in the case when the distance between two atoms is smaller than the wavelength of the field, the ...
Tópico(s): Spectroscopy and Quantum Chemical Studies
2011 - IOP Publishing | Journal of Physics B Atomic Molecular and Optical Physics
Dumitru Chesov, V. Lesanu, Nelly Ciobanu, Alexandru Codreanu, Valeru Crudu, Luís E. Cuevas, Dániel Kiss, N. Czoboly, Ákos Somoskövi,
Tópico(s): Biosimilars and Bioanalytical Methods
2020 - International Union Against Tuberculosis and Lung Disease | The International Journal of Tuberculosis and Lung Disease
LeAnna L. Cates, Alexandru Codreanu, Nelly Ciobanu, Heather Fosburgh, Christopher J. Allender, Heather Centner, David M. Engelthaler, Valeru Crudu, Ted Cohen, Nick A Menzies,
BACKGROUND Diagnosing drug resistance is critical for choosing effective TB treatment regimens. Next-generation sequencing (NGS) represents an alternative approach to conventional phenotypic drug susceptibility testing (pDST) for diagnosing TB drug resistance. METHODS We undertook a budget impact analysis estimating the costs of introduction and routine use of NGS in the Moldovan National TB Programme. We conducted an empirical costing study and collated price and operating characteristics for NGS ...
Tópico(s): Biosimilars and Bioanalytical Methods
2022 - International Union Against Tuberculosis and Lung Disease | The International Journal of Tuberculosis and Lung Disease
Lyndon P. James, Fayette Klaassen, Sedona Sweeney, Jennifer Furin, Molly F. Franke, Reza Yaesoubi, Dumitru Chesov, Nelly Ciobanu, Alexandru Codreanu, Valeriu Crudu, Ted Cohen, Nicolas A. Menzies,
Background Emerging evidence suggests that shortened, simplified treatment regimens for rifampicin-resistant tuberculosis (RR-TB) can achieve comparable end-of-treatment (EOT) outcomes to longer regimens. We compared a 6-month regimen containing bedaquiline, pretomanid, linezolid, and moxifloxacin (BPaLM) to a standard of care strategy using a 9- or 18-month regimen depending on whether fluoroquinolone resistance (FQ-R) was detected on drug susceptibility testing (DST). Methods and findings The primary ...
Tópico(s): Pharmacogenetics and Drug Metabolism
2024 - Public Library of Science | PLoS Medicine
Naomi Hillery, Marva Seifert, Donald G. Catanzaro, Symone McKinnon, Rebecca E. Colman, Peter G. Chiles, Dumitru Chesov, Nelly Ciobanu, Christopher Hagan, Valeriu Crudu, Antonino Catanzaro, Timothy C. Rodwell,
The lack of accurate and efficient diagnostic devices for extensively drug-resistant tuberculosis (XDR-TB) makes it a severe threat to global public health. A prospective clinical study in an intended-use cohort was designed to evaluate the Akonni Biosystems XDR-TB TruArray and lateral flow cell (XDR-LFC) to address this gap in tuberculosis diagnostics.
Tópico(s): Innovative Microfluidic and Catalytic Techniques Innovation
2021 - JMIR Publications | JMIR Research Protocols
Valeriu Crudu, Dumitru Chesov, Alexandru Codreanu, Nadejda Turcanu, Nelly Ciobanu, Liuba Nepoliuc, Doina Rusu,
Tópico(s): Mycobacterium research and diagnosis
2025 - Elsevier BV | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
N. A. Enaki, Marina Ţurcan, Nelly Ciobanu, Tudor Roşca, Ashok Vaseashta,
The second-order coherent function at two-photon cooperative emission is investigated. As was observed the co-operative generation of γ-ray light is possible if the duration of X-ray pulse is longer than the co-operative delay super-radiant time of gamma photons. In this case, the amplitude of the X-ray field is constant in the process of co-operative γ-photon emission and the quasi-energetic levels for the extended nuclei system are dressed. Achievement of viable two-photon sources of correlated ...
Tópico(s): Quantum Mechanics and Applications
2011 - Springer Nature (Netherlands) | NATO science for peace and security series. A, Chemistry and biology
Nelly Ciobanu, N. A. Enaki, Miguel Orszag,
The effect of standing wave amplitude on the resonance fluorescence of a three-level system of radiators is discussed. The cooperative emission and the fluorescence spectrum of emitted photons from two atoms situated in the nodes and anti-nodes of the laser field are investigated. A realizable model for producing an entangled state, that can be controlled via the distance of localization between two atoms in the anti-nodes, is discussed.
Tópico(s): Quantum optics and atomic interactions
2012 - IOP Publishing | Physica Scripta
Nelly Ciobanu, N. A. Enaki, Miguel Orszag,
The resonance fluorescence emitted by two three-level atoms dressed in a strong laser field is studied. A stark dynamics shift based on photon statistics is discussed. The appearance of coherent Rabi oscillations (quantum beats) of the atoms located in the non-symmetric positions of the anti-nodes and the modification of the quantum correlations at the Stokes and anti-Stokes cooperative emission are described. For some positions of the atoms, we observed the violation of the Cauchy–Schwarz inequality, ...
Tópico(s): Quantum Mechanics and Applications
2013 - IOP Publishing | Journal of Physics B Atomic Molecular and Optical Physics
Nelly Ciobanu, N. A. Enaki, Miguel Orszag,
The possibility of manipulating the position of atoms dressed in the standing field of the cavity, based on the resonance fluorescence emission, is discussed. Stark dynamics modification and the strong coupling between two undistinguishable radiators situated in non-symmetric positions of the anti-nodes are investigated. The fluorescent spectrum is shown versus the second atom localization and external field parameters. We describe the conditions in which the Rabi frequencies of atoms change their ...
Tópico(s): Laser-Matter Interactions and Applications
2013 - IOP Publishing | Physica Scripta
Nelly Ciobanu, Spiridon Rusu, Vasile Tronciu,
We discuss a model of anticipated synchronization of two distributed feedback lasers (DFB) with passive dispersive reflector. The influence of passive dispersive reflector parameters on the anticipated synchronization is discussed. The conditions for delayed, perfect and anticipated synchronizations are explored in detail.
Tópico(s): Quantum optics and atomic interactions
2015 - Springer Nature | IFMBE proceedings
Nelly Ciobanu, Victor Vovc, A. Saulea, Vasile Tronciu,
In this paper we give a model that describes the nonlinear cooperative stationary phenomena at interaction of Bose-condensed phonons with millimeter or terahertz electromagnetic radiation in a biological media. The expressions that characterize the real and imaginary parts of the dielectric susceptibility and permeability, as well as the refraction and reflection indexes caused by condensed phonons were obtained.
Tópico(s): Biofield Effects and Biophysics
2015 - Springer Nature | IFMBE proceedings
Victor Vovc, Svetlana Lozovanu, Andrei Ganenco, Nelly Ciobanu,
This article presents the design and content of biomedical disciplines included in biomedical engineering master’s program for universities of the Republic of Moldova. The biomedical curriculum of the biomedical education program contains two fundamental disciplines: 1.Functional anatomy and physiology; 2.Biophysics. To make more familiar future biomedical engineers with technical part of medical practice one applied discipline is proposed: Diagnostics and treatment methods in medicine.
Tópico(s): Biomedical and Engineering Education
2015 - Springer Nature | IFMBE proceedings
Elena Chesov, Greta Bălan, Nelly Ciobanu, Dumitru Chesov, Valeriu Crudu,
Background: Resistance profile of the index case is recommended to be considered in treatment decision in close contacts with active TB, pending drug sensitivity test (DST) results. However, the agreement between index and secondary TB cases DST could be not enough accurate in special in settings with a high rate of infection transmission. Aim: To compare the resistance profiles of M. tuberculosis in index and secondary TB cases registered in the same household. Methods: We performed a retrospective study to ...
Tópico(s): Infectious Diseases and Tuberculosis
2018 - Elsevier BV | Tuberculosis
Nelly Ciobanu, Sofia Alexandru, Dumitru Chesov, Alexandru Codreanu, Christoph Lange, Valeriu Crudu,
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Tópico(s): Traditional Chinese Medicine Studies
2018 - Sankt-Peterburg : NIIÈM imeni Pastera | Russian Journal of Infection and Immunity
Valeriu Crudu, Sofia Alexandru, Alexandru Codreanu, Nelly Ciobanu, Dumitru Chesov, Valentina Vilc, Ana Donica, Elena Romancenco, Ecaterina Noroc, Nadejda Turcan, Christoph Lange,
Introduction: With increasing incidence of MDRTB accurate drug susceptibility testing (DST) of M.tuberculosis has become crucial for proper patient management. Aim: to evaluate the concordance across phenotypic and genotypic results of DST of M.tuberculosis for rapid diagnostic of MDRTB. Methods: Results across phenotypic (MGIT 960 system) and molecular methods (GenoType MTBDRplus&GeneXpert MTB/RIF) were evaluated to find the discrepancies in the DST results. Results: Among 1119 TB patients investigated by ...
Tópico(s): Mycobacterium research and diagnosis
2018 - Elsevier BV | Tuberculosis