Kuanren Qian, Ashlee S. Liao, Shixuan Gu, Victoria A. Webster‐Wood, Yongjie Zhang,
... neurons undergo to develop sophisticated morphologies and interwoven neurite networks. Recent experimental research advances have enabled us ... computationally expensive. In this paper, we incorporate experimental neurite features into a phase field method-based neuron ... Based on a semi-automated quantitative analysis of neurite morphology, we obtain relative turning angle, average tortuosity, neurite endpoints, average segment length, and the total length of neurites. We use the total neurite length to determine ...
Tópico(s): Model Reduction and Neural Networks
2023 - Elsevier BV | Computer Methods in Applied Mechanics and Engineering
Jungeun Lee, Beate I. Escher, Stefan Scholz, Rita Schlichting,
... The impairment of key neurodevelopmental processes such as neurite outgrowth inhibition can be used as endpoints for screening of DNT effects. We quantified neurite-specific effects using the ratio of effect concentrations for cytotoxicity and neurite outgrowth inhibition (SRcytotoxicity). Baseline cytotoxicity, the minimal toxicity ... cytotoxicity with the effects on cell viability and neurite outgrowth, respectively. The effects on cell viability and neurite length were measured based on image analysis in ...
Tópico(s): Neuroscience and Neuropharmacology Research
2022 - Springer Science+Business Media | Archives of Toxicology
Zubair Ahmed, Richard I. Tuxworth,
... target and promoted dorsal root ganglion neuron (DRGN) neurite outgrowth in vitro and stimulated axon regeneration after ... the effects of AZD1390 on DRGN survival and neurite outgrowth. The treatment of DRGN cultures with increasing ... and 81% increase in DRGN survival, % DRGN with neurites, and longest neurite length compared to vehicle-treated DRGN, respectively (Figure ... showed similar levels of DRGN survival, % DRGN with neurites, and the mean longest neurite length as observed with AZD1390 (Figure 2C–F). ...
Tópico(s): Nerve injury and regeneration
2022 - Springer Science+Business Media | Clinical and Translational Medicine
Shigeru Tanaka, Naoto Shimada, Hiroko Shiraki, Tatsuhiro Miyagi, Kana Harada, Izumi Hide, Norio Sakai,
During neuronal development, immature neurons extend neurites and subsequently polarize to form an axon and dendrites. We have previously reported that G protein-coupled receptor 3 (GPR3) levels increase ... neuronal development, and that GPR3 has functions in neurite outgrowth and neuronal differentiation in cerebellar granular neurons. ... is transported and concentrated at the tips of neurite, thereby contributing to the local activation of protein ... PKA). However, the signaling pathways for GPR3-mediated neurite outgrowth and its subsequent effects on neuronal polarization ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2021 - Elsevier BV | Molecular and Cellular Neuroscience
Elveda Gozdas, Hannah Fingerhut, Lauren Dacorro, Jennifer L. Bruno, S. M. Hadi Hosseini,
... major risk factor for neurodegenerative diseases and affects neurite distributions throughout the brain, yet underlying neurobiological mechanisms remain unclear. Multi-shell diffusion-weighted imaging and neurite orientation dispersion and density imaging (NODDI) now provide ... the cortex and white matter. In this study, neurite distributions were evaluated in the cortex and white ... and neurodegeneration. Older age was associated with reduced neurite density and neurite orientation dispersion (ODI) in widespread ...
Tópico(s): MRI in cancer diagnosis
2021 - Oxford University Press | Cerebral Cortex
Evy Lefevere, Inge Van Hove, Jurgen Sergeys, David Steel, Reinier O. Schlingemann, Lieve Moons, Ingeborg Klaassen,
... eventual blindness. During the formation of these membranes, neurite/process outgrowth occurs in retinal neurons and glial ... vitreous of PDR patients with FVMs, could induce neurite outgrowth in an experimental setting.Retinal explants of ... performed with the addition of potential inducers of neurite outgrowth. Automated analysis of neurite outgrowth was performed by measuring β-tubulin-immunopositive neurites using Image J. Expression of PDGF receptors was ... PDR patients.Platelet-derived growth factor (PDGF) induced neurite outgrowth in a concentration-dependent manner, whilst neuregulin ...
Tópico(s): Retinal Development and Disorders
2021 - Taylor & Francis | Current Eye Research
Shuaizhang Li, Li Zhang, Ruili Huang, Tuan Xu, Fred Parham, Mamta Behl, Menghang Xia,
... process that has been implicated in neurodevelopment is neurite outgrowth; the disruption of which can result in ... we developed a green fluorescent protein (GFP) labeled neurite outgrowth assay in a high-content, high-throughput ... compounds that have previously been screened in other neurite outgrowth assays. This library consists of known developmental ... 24 and 48 h. Effects of compounds on neurite outgrowth were evaluated by quantifying total neurite length, number of segments, and maximum neurite length ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2021 - Elsevier BV | NeuroToxicology
Jessica L. Funnell, Alexis M. Ziemba, James F. Nowak, Hussein Awada, Nicos Prokopiou, Johnson Samuel, Yannick Guari≠, Benjamin Nottelet, Ryan J. Gilbert,
... grafted scaffolds combined with magnetic fields to promote neurite outgrowth by providing contact guidance from the aligned ... stimulation from the SPIONs in the magnetic field. Neurite outgrowth from primary rat dorsal root ganglia (DRG) ... determine the optimal magnetic field stimulation to promote neurite outgrowth, we generated a static, alternating, and linearly ... scaffold over time. The alternating magnetic field increased neurite length by 40% on control fibers compared to ... magnetic field resulted in a 30% increase in neurite length and 62% increase in neurite area on ...
Tópico(s): Graphene and Nanomaterials Applications
2021 - Elsevier BV | Acta Biomaterialia
Reza Rahmanzadeh, Po‐Jui Lu, Muhamed Baraković, Matthias Weigel, Pietro Maggi, Thanh D. Nguyen, Simona Schiavi, Alessandro Daducci, Francesco La Rosa, Sabine Schaedelin, Martina Absinta, Daniel S. Reich, Pascal Sati, Yi Wang, Meritxell Bach Cuadra, Ernst-Wilhelm Radüe, Jens Kühle, Ludwig Kappos, Cristina Granziera,
... the study. Differences in myelin water fraction and neurite density index were substantial when lesions were compared ... lesions exhibited a decreased myelin water fraction and neurite density index compared with healthy (P < 0.0001) ... Periventricular lesions showed decreased myelin water fraction and neurite density index compared with lesions in the juxtacortical ... paramagnetic rims showed decreased myelin water fraction and neurite density index relative to lesions without a rim ( ... 75% of white matter lesions, the reduction in neurite density index was higher than the reduction in ...
Tópico(s): Bone and Joint Diseases
2021 - Oxford University Press | Brain
Produção Nacional
Revisado por pares
Gary Hin‐Fai Yam, Francisco Bandeira, Yuchi Liu, Kavya Devarajan, Nur Zahirah Binte M. Yusoff, Hla-Myint Htoon, Jodhbir S. Mehta,
... investigate the effect of lenticule customization on lenticule neurite length profile and the excitatory response (calcium signaling) ... by sodium dodecyl sulfate (SDS), respectively. The overall neurite scaffold immuno-positive to TuJ1 (neuron-specific class III β-tubulin) expression and population of active neurite fragments with calcium response revealed by L-glutamate- ... construction. The NeuronJ plugin was used to measure neurite lengths for TuJ1 (NL-TuJ1) and calcium signal ( ... Whitney U test.The customization methods significantly eliminated neurites inside the lenticules. NL-TuJ1 was significantly reduced ...
Tópico(s): Corneal Surgery and Treatments
2021 - Elsevier BV | Journal of Advanced Research
Gavin P. Winston, Sjoerd B. Vos, Benoît Caldairou, Seok‐Jun Hong, Monika Czech, Tobias C. Wood, Stephen Wastling, Gareth J. Barker, Boris C. Bernhardt, Neda Bernasconi, John S. Duncan, Andrea Bernasconi,
... spatial distribution of conventional and novel metrics including neurite density derived from NODDI (Neurite Orientation Dispersion and Density Imaging) and myelin water ... weighted MRI. Conventional diffusion and novel metrics of neurite density and MWF were sampled from intracortical grey ... frontopolar cortices with more restricted areas of reduced neurite density. Diffusivity increases were largely related to reductions in neurite density, and to a lesser extent CSF partial ... were seen. These were primarily related to reduced neurite density, with an additional relationship to reduced MWF ...
Tópico(s): Advanced MRI Techniques and Applications
2020 - Elsevier BV | NeuroImage Clinical
Valeria Kaltezioti, Iosifina P. Foskolou, Matthieu D. Lavigne, Elpinickie Ninou, Matina Tsampoula, Maria Fousteri, Marigoula Margarity, Panagiotis Politis,
... system (CNS) development, proper and timely induction of neurite elongation is critical for generating functional, mature neurons, ... transcription factor, as a key player in inhibiting neurite elongation. Although Prox1 promotes acquisition of early neuronal ... differentiated neurons, indicating a regulatory role in delaying neurite outgrowth in newly formed neurons. Consistently, we show that Prox1 is sufficient to inhibit neurite extension in mouse and human neuroblastoma cell lines. More importantly, Prox1 overexpression suppresses neurite elongation in primary neuronal cultures as well as ...
Tópico(s): Cellular Mechanics and Interactions
2020 - Springer Nature | Cellular and Molecular Life Sciences
Issa Olakunle Yusuf, Hsiu-Mei Chen, Pei-Hsun Cheng, C.S. Chang, Shaw‐Jenq Tsai, Jih‐Ing Chuang, Chia-Ching Wu, Bu‐Miin Huang, H. Sunny Sun, Chuan‐Mu Chen, Shang‐Hsun Yang,
... is a neurodegenerative disease that causes deficits in neurite outgrowth, which suggests that enhancement of neurite outgrowth is a potential direction by which to ... pathway, and FGF9 also stimulates cytoskeletons to enhance neurite outgrowth via nuclear factor kappa B (NF-kB) ... the importance of the ERK pathway for the neurite outgrowth induced by FGF9 in HD striatal models. ... Q111 striatal knock-in cell lines to examine neurite outgrowth, cytoskeletal markers, and synaptic proteins via immunofluorescence ...
Tópico(s): Ubiquitin and proteasome pathways
2020 - Elsevier BV | Life Sciences
Hongxiu Han, Chunxue Yang, Yuan Zhang, Changhao Han, Guohua Zhang,
... ERK), and p38 inhibitors significantly attenuated VEGF-induced neurite elongation. These findings provide direct evidence that VEGF, ... ERK), and p38 inhibitors significantly attenuated VEGF-induced neurite elongation. These findings provide direct evidence that VEGF, ... mitogen-activated protein kinases (MAPKs) on VEGF-induced neurite outgrowth, the cells were treated overnight with PI3K/ ... plus VEGF (100 ng/mL). To assess the neurite outgrowth, the tubulin immunostaining of DRG neurons was ... were set up for each condition. The longest neurite length measurement of neurons was performed with Leica ...
Tópico(s): Angiogenesis and VEGF in Cancer
2020 - Elsevier BV | American Journal Of Pathology
Sudhin Thampi, Anoopkumar Thekkuveettil, Vignesh Muthuvijayan, P. Ramesh,
... a great impact on the directional outgrowth of neurites. In this study, we have electrospun electrically conductive, ... neuronal cell behavior, including cell adhesion, proliferation, and neurite outgrowth. These bio-adhesion proteins or nanomaterials mimicking ... attributes of these scaffolds or may alter the neurite outgrowth of PC-12 cells like any other ... lysine (PLL) for neural cell adhesion, proliferation, and neurite extension. Neurite outgrowth studies showed that although the average neurite length was comparable on both GO- and PLL- ...
Tópico(s): Neuroscience and Neural Engineering
2020 - American Chemical Society | ACS Applied Bio Materials
Jingcong Zhuang, Zhongjie Chen, Pingping Cai, Rong Wang, Qingwei Yang, Longling Li, Hui‐Li Yang, Renjing Zhu,
... as well as their effects on cell apoptosis, neurite outgrowth, and inflammation in Alzheimer disease (AD). Rat ... cellular models alone or combined. Then, cell apoptosis, neurite outgrowth, proinflammatory cytokines, miR-125b, FOXQ1, PTGS2, and CDK5 expressions were detected. MiR-125b inhibition facilitated neurite outgrowth but suppressed cell apoptosis and proinflammatory cytokines ( ... promoted cell apoptosis and proinflammatory cytokines but repressed neurite outgrowth; PTGS2 inhibition achieved the opposite effects; CDK5 ...
Tópico(s): Genomics, phytochemicals, and oxidative stress
2020 - Frontiers Media | Frontiers in Cellular Neuroscience
Muzhou Jiang, Jie Meng, Fan Zeng, Hong Qing, Gregory Hook, Vivian Hook, Zhou Wu, Junjun Ni,
Abstract Lysosomes are known to mediate neurite outgrowth in neurons. However, the principal lysosomal molecule controlling that outgrowth is unclear. We studied primary mouse neurons in vitro and found that they naturally develop neurite outgrowths over time and as they did so ... neurons with CA‐074Me, which inhibits CTSB, prevented neurites. As that compound also inhibits another protease, we ... knockout, KO) using CRISPR technology and induced their neurite outgrowth by treatment with retinoic acid. We found ...
Tópico(s): Lipid Membrane Structure and Behavior
2020 - Wiley | Journal of Neurochemistry
Elisabetta Mantuano, Pardis Azmoon, Michael A. Banki, Michael S. Lam, Christina J. Sigurdson, Steven L. Gonias,
... initiated cell-signaling. S-PrP promoted PC12 cell neurite outgrowth. This response required the NMDA-R, LRP1, ... The effects of S-PrP on PC12 cell neurite outgrowth and Schwann cell migration were similar to ... initiated cell-signaling. S-PrP promoted PC12 cell neurite outgrowth. This response required the NMDA-R, LRP1, ... The effects of S-PrP on PC12 cell neurite outgrowth and Schwann cell migration were similar to ... lipid rafts to activate p59fyn and to enhance neurite outgrowth.J. Cell Biol. 2005; 169 (15851519): 341- ...
Tópico(s): Nuclear Receptors and Signaling
2020 - Elsevier BV | Journal of Biological Chemistry
Sumei Li, Jifeng Zhang, Jiaqi Zhang, Jiong Li, Longfei Cheng, Li Chen, Caihui Cha, Guoqing Guo,
... aims to revealing the underlying microtubule mechanism of neurites outgrowth during neuronal development and also proposes a ... Microtubule polymerization and severing form the basis for neurite outgrowth and branch formation. However, the mechanisms that ... of microtubules are unclear. Here, we showed that neurite outgrowth mediated by collapsing response mediator protein 2 ( ... the severing of microtubule cytoskeleton.To explore whether neurite outgrowth was mediated by coordination of CRMP2 and ... was also performed to determine the morphology of neurites.We first demonstrated that CRMP2 interacted with spastin ...
Tópico(s): Microtubule and mitosis dynamics
2020 - Bentham Science Publishers | CNS & Neurological Disorders - Drug Targets
Jeremiah D. Paskus, Bruce E. Herring, Katherine W. Roche,
... exchange factor domains of kalirin in regulation of neurite growth and neuronal morphology.J. Neurosci. 2001; 21: ... studies have established the importance of Trio in neurite growth and axon guidance. Trio has been shown to function in neurite formation downstream of the neurotrophin nerve growth factor ( ... are involved in the NGF pathway, leading to neurite outgrowth.Curr. Biol. 2002; 12: 307-312Abstract Full ... interpret, as extensions in PC12 cells are not neurites precisely. However, the NGF downstream target Kidins220 has ...
Tópico(s): Mitochondrial Function and Pathology
2020 - Elsevier BV | Trends in Neurosciences
Tao Jiang, Zhenbin Cai, Zhisheng Ji, Jianyu Zou, Zhi Liang, Guowei Zhang, Yaozhong Liang, Hongsheng Lin, Minghui Tan,
Spastin, a microtubule-severing enzyme, is important for neurite outgrowth. However, the mechanisms underlying the post-transcriptional ... 1 (MALAT1)/microRNA-30 (miR-30) axis during neurite outgrowth. The miR-30 expression level decreased in ... 30 overexpression suppressed while miR-30 inhibition promoted neurite outgrowth in hippocampal neurons. Spastin was validated as ... assay. The protein expression, microtubule severing activity, and neurite promoting effect of spastin were suppressed by the ... by miR-30 inhibitors. MALAT1 expression increased during neurite outgrowth and MALAT1 silencing impaired neurite outgrowth. miR- ...
Tópico(s): RNA Research and Splicing
2020 - Frontiers Media | Frontiers in Cellular Neuroscience
Kai A. Kropp, Alberto Domingo López-Muñoz, Birgit Ritter, Rocı́o Martı́n, Alberto Rastrojo, Sangar Srivaratharajan, Katinka Döhner, Akshay Dhingra, Julia S. Czechowicz, Claus‐Henning Nagel, Beate Sodeik, Antonio Alcamı́, Abel Viejo‐Borbolla,
... HSV-2) replicates in epithelial cells and enters neurites to infect neurons of the peripheral nervous system. ... factors and attractive and repulsive directional cues influence neurite outgrowth and neuronal survival. We hypothesized that HSV- ... modulates the activity of such cues to increase neurite outgrowth. To test this hypothesis, we exposed sensory ... HEK-293T cells, since they express repellents of neurite outgrowth. We show that HEK-293T cells secrete factors that inhibit neurite outgrowth, while infection with HSV-2 strains MS ...
Tópico(s): Nerve injury and regeneration
2020 - American Society for Microbiology | Journal of Virology
Kazutoshi Hisano, Shiori Kawase, Tetsuhiko Mimura, Hironori Yoshida, Hiroki Yamada, Hisao Haniu, Tamotsu Tsukahara, Taiga Kurihara, Yoshikazu Yonei, Naoto Saito, Takeshi Uemura,
Neurite outgrowth is important in neuronal circuit formation and functions, and for regeneration of neuronal networks following ... identification and characterization of the molecules that regulate neurite outgrowth are essential for understanding how brain circuits ... LPE) and stearoyl-LPE (18:0 LPE), stimulate neurite growth in cultured cortical neurons. Interestingly, YM-254890, ... Gq/11 protein, inhibited 16:0 LPE-stimulated neurite outgrowth but not 18:0 LPE-stimulated neurite outgrowth. In contrast, pertussis toxin, an inhibitor of ...
Tópico(s): Lipid Membrane Structure and Behavior
2020 - Elsevier BV | Biochemical and Biophysical Research Communications
Brigid K. Jensen, Martin H Schuldi, Kevin McAvoy, K. Russell, Ashley Boehringer, Bridget M Curran, Karthik Krishnamurthy, Xinmei Wen, Thomas Westergard, Le Ma, Aaron R. Haeusler, Dieter Edbauer, Piera Pasinelli, Davide Trotti,
... demonstrate that mobile GA aggregates are present within neurites, evoke a reduction in synaptic vesicle-associated protein ... and rescues neuronal toxicity. Mobile GA aggregates in neurites of cortical and motor neurons result in increased ... GA expression in primary neurons results in reduced neurite outgrowth and cellular toxicity through proteasome impairment and ... soma, eGFP-GA inclusions were also present within neurites (Wen et al, 2014). This localization pattern was ... determine whether poly-GA aggregates are found in neurites over time, we performed a longitudinal assessment of ...
Tópico(s): Genetic Neurodegenerative Diseases
2020 - Springer Nature | EMBO Molecular Medicine
Chenju Yi, Ket Yin Goh, Lik‐Wei Wong, Ajeena Ramanujan, Kazuhiro Tanaka, Sreedharan Sajikumar, Carlos F. Ibáñez,
... species, amyloid plaque burden, gliosis, mitochondrial stress, and neurite dystrophy than global knock-outs. Strikingly, long-term ... species, amyloid plaque burden, gliosis, mitochondrial stress and neurite dystrophy than global knock-outs. Long-term synaptic ... age, achieve substantial Aβ burden with gliosis and neurite degeneration by 4 months, and show significant memory ... versus vehicle (Student's t-test). Quantification of neurite length in cultured cortical neurons of the indicated ... or 10 µM). Data are expressed as mean neurite length in individual neurons, relative to wild-type ...
Tópico(s): Computational Drug Discovery Methods
2020 - Springer Nature | The EMBO Journal
Alessandro Falconieri, Sara De Vincentiis, Vittoria Raffa,
... by protecting them from fast degradation and demonstrating neurite elongation and increased complexity of the neuronal branching ... manipulate axonal growth and guidance. Interestingly, by labeling neurites with MNPs, the extremely low forces generated by ... magnetic field can be used for stretching the neurites, resulting in elongation and growth by a mechanism ... guidance, by orientating the direction of growth of neurites [6]. When the magnetic field is applied to ... was found to modulate growth cone motility and neurite growth [8]. D-Mannose-coated MNPs were used ...
Tópico(s): Neuroscience and Neuropharmacology Research
2019 - Future Medicine | Nanomedicine
Anthony R. D’Amato, Devan L. Puhl, Alexis M. Ziemba, Christopher Johnson, Janneke Doedee, Jonathan Bao, Ryan J. Gilbert,
... or fibers coated with laminin. On uncoated fibers, neurite outgrowth was restricted by fibers displaying divoted or pitted nanotopography when compared to neurite outgrowth on smooth fibers. However, neurites extending from whole DRG cultured on laminin-coated ... restricted by divoted or pitted surface nanotopography. Thus, neurites extending on laminin-coated fibers were able to extend long neurites even in the presence of surface divots or ... exhibited a 1.5-fold increase in total neurite length, and a 2.3 or 2.7- ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2019 - Public Library of Science | PLoS ONE
Takeshi Endo, Ken Kadoya, Daisuke Kawamura, Norimasa Iwasaki,
... importance? This study demonstrates that Schwann cells stimulate neurite outgrowth by direct contact with neurites and by secreting factors. Notably, the effect of cell-contact factors in neurite outgrowth is comparable to that of secreted factors, ... cell surface molecules on Schwann cells that promote neurite outgrowth could lead to development of a new ... effect of SCs on promotion of neuronal outgrowth. Neurite outgrowth of adult sensory neurons from dorsal root ... in a 51% reduction in the length of neurite outgrowth. In addition, when dorsal root ganglion neurons ...
Tópico(s): Pain Mechanisms and Treatments
2019 - Wiley | Experimental Physiology
Produção Nacional
Revisado por pares
Francisco Bandeira, Gary Hin‐Fai Yam, Yu‐Chi Liu, Kavya Devarajan, Jodhbir S. Mehta,
We study the density and excitatory response of neurites, and Schwann cells (SCs) in fresh and cryopreserved ... microscopy, followed by three-dimensional reconstruction, to reveal neurite distribution. The lenticule neurite density (LND) was assessed using a validated neurite tracing and length measurement method with NeuronJ. LND ... marker expression and the laser effect on SC-neurite interaction was examined under transmission electron microscopy (TEM). ... functional excitatory calcium response assay.Using a validated neurite length measurement method, we found an inverse correlation ...
Tópico(s): Corneal surgery and disorders
2019 - Cadmus Press | Investigative Ophthalmology & Visual Science
... which neurons initially extend an excess number of neurites, the majority of which will be eliminated by the mechanism called neurite pruning. Some neurites undergo stereotyped and developmentally regulated pruning. However, the signaling cues that instruct stereotyped neurite pruning are yet to be fully elucidated. Here we show that Wnt morphogen instructs stereotyped neurite pruning for proper neurite projection patterning of the cholinergic motor neuron called ... and lin-17/frizzled mutant animals, the PDB neurites often failed to prune and grew towards the ...
Tópico(s): Spaceflight effects on biology
2019 - eLife Sciences Publications Ltd | eLife