Tatsuro Nakagawa, Maurizio Pellegrino, Koji Sato, Leslie B. Vosshall, Kazushige Touhara,
... odor- or pheromone-specific Or subunit and the Orco co-receptor, which is highly conserved in all insect species. The insect Or-Orco complex has been proposed to function as a ... possibly modulated by G-proteins. However, the Or-Orco proteins lack homology to any known family of ... the mechanisms by which odors activate the Or-Orco complex and how ions permeate this complex remain ... To begin to address the relationship between Or-Orco structure and function, we performed site-directed mutagenesis ... or Tyr residues in the silkmoth BmOr-1-Orco pheromone receptor complex and measured functional properties of ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2012 - Public Library of Science | PLoS ONE
... subunits and a highly conserved co-receptor subunit (Orco), assembled in an unknown stoichiometry. Synthetic Orco directed agonists and antagonists have recently been identified. Several Orco antagonists have been shown to act via an ... by odorants. The high degree of conservation of Orco across insect species results in Orco antagonists having broad activity at ORs from a ... species and suggests that the binding site for Orco ligands may serve as a modulatory site for ... of biogenic and trace amines, identifying several as Orco antagonists. Of particular interest were tryptamine, a plant- ...
Tópico(s): Insect Pest Control Strategies
2014 - Faculty of 1000 | F1000Research
Latha Mukunda, Sofía Lavista-Llanos, Bill S. Hansson, Dieter Wicher,
... proteins, an odor-specific OrX and a coreceptor (Orco) protein. These ORs form ligand gated cation channels ... is regulated by intracellular signaling cascades. Heterologously expressed Orco proteins form also non-selective cation channels that ... such as VUAA1. The stoichiometry of OR or Orco channels is unknown. In this study we engineered the simplest oligomeric construct, the Orco dimer (Orco di) and investigated its functional properties. Two Orco proteins were coupled via a 1-transmembrane protein ...
Tópico(s): Insect Utilization and Effects
2014 - Frontiers Media | Frontiers in Cellular Neuroscience
Rebecca M. Turner, Stephen Derryberry, Brijesh Kumar, Thomas Brittain, Laurence J. Zwiebel, Richard D. Newcomb, David L. Christie,
... and a highly conserved co-receptor known as Orco. Orco is found only in insects, and very little ... channel activation. In the absence of an ORx, Orco forms homomeric channels that can be activated by a synthetic agonist, VUAA1. Drosophila melanogaster Orco (DmelOrco) contains eight cysteine amino acid residues, six ... cysteine residues with serine or alanine and expressed Orco mutants in Flp-In 293 T-Rex cells. ... Kinetic modeling indicated that the response of the Orco mutants to VUAA1 was faster than wild-type ...
Tópico(s): Olfactory and Sensory Function Studies
2014 - Elsevier BV | Journal of Biological Chemistry
Matthew DeGennaro, Carolyn S. McBride, Laura F. Seeholzer, Takao Nakagawa, Emily Jane Dennis, Chloe Goldman, Nijole Jasinskiene, Anthony A. James, Leslie B. Vosshall,
Mosquitoes with null mutations in the orco olfactory co-receptor have reduced preference for humans, are only attracted to human odour in the presence of CO2 and are not ... no longer prefer humans. The mutation disrupts the orco gene, which codes for a co-receptor that ... essential for all insect odorant receptors. Interestingly the orco-mutant female mosquitoes were attracted to humans even ... selective subunit and an obligate co-receptor called Orco (ref. 6). Here we use zinc-finger nucleases to generate targeted mutations in the orco gene of A. aegypti to examine the contribution ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2013 - Nature Portfolio | Nature
... common subunit (the odorant receptor co-receptor subunit, Orco) and one of many variable subunits that confer odorant specificity. The recent discovery of Orco directed ligands, including both agonists and antagonists, suggests Orco as a promising target for chemical control of ... In addition to competitively inhibiting OR activation by Orco agonists, several Orco antagonists have been shown to act through a ... by odorants. We previously identified a series of Orco antagonists, including N-(4-ethylphenyl)-2-thiophenecarboxamide (OX1a, ...
Tópico(s): Insect and Pesticide Research
2013 - Public Library of Science | PLoS ONE
Brijesh Kumar, Robert W. Taylor, Gregory M. Pask, Laurence J. Zwiebel, Richard D. Newcomb, David L. Christie,
... sensitive tuning receptor, and a conserved co-receptor (Orco). An Orco agonist, VUAA1, is able to activate both heteromeric and homomeric Orco-containing channels. Very little is known about specific residues in Orco that contribute to cation permeability and gating. We ... 7, for channel function by mutagenesis. Drosophila melanogaster Orco and its substitution mutants were expressed in HEK ... a substantial reduction in channel activity. The D466E Orco substitution mutant was ∼2 times more sensitive to ...
Tópico(s): Plant and animal studies
2013 - Public Library of Science | PLoS ONE
Suhyoung Bahk, Walton D. Jones,
... an odorant-specific odorant receptor (OR) along with Orco, the olfactory co-receptor. Orco binds ORs and permits their trafficking to the ... cation channels. While most amino acid residues in Orco are well conserved across insect orders, one especially well-conserved region in Orco's second intracellular loop is a putative calmodulin ( ... In this study, we explore the relationship between Orco and CaM in vivo in the olfactory neurons ... disrupts the spontaneous firing of OSNs and reduces Orco trafficking to the ciliated dendrites of OSNs without ...
Tópico(s): Plant biochemistry and biosynthesis
2016 - BioMed Central | BMC Biology
Devin Kepchia, Scott Moliver, Kunal Chohan, Cameron Phillips, Charles W. Luetje,
... subunit and a conserved odorant receptor co-receptor (Orco) subunit, in an unknown stoichiometry. The Orco subunit possesses an allosteric site to which modulators ... characterized several halogen-substituted versions of a phenylthiophenecarboxamide Orco antagonist structure. Orco antagonist activity was assessed on ORs from Drosophila ... activated by diverse odorants. Next, we asked whether Orco antagonist OX1w could affect insect olfactory behavior. A ... closed experimental chamber. Attraction to ethyl acetate was Orco dependent and also required the odorant specificity subunit ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2017 - Public Library of Science | PLoS ONE
Alan Soffan, Siti Subandiyah, Hirokazu Makino, Tomoaki Watanabe, Tokumasa Horiike,
Odorant coreceptor (Orco) represents one of the essential genes in the insect olfactory system, which facilitates signal transduction and heterodimerization with ... is important to examine the functional flexibility of Orco that potentially supports insect survival. The maximum likelihood ... in PAML ver 4.9e package across 59 Orco codon sequences available from GenBank. These sequences represented ... site model that identified common ω values for Orco, it was clearly shown that Orco was under strong purifying selection, indicated by the ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2018 - Oxford University Press | Journal of Insect Science
Devin Kepchia, Pingxi Xu, Raymond Terryn, Ana Hortência Fônseca Castro, Stephan C. Schürer, Walter Leal Filho, Charles W. Luetje,
... ORs) contain a conserved odorant receptor co-receptor (Orco) subunit which is an attractive target for repellent development. Orco directed antagonists allosterically inhibit odorant activation of ORs and we previously showed that an airborne Orco antagonist could inhibit insect olfactory behavior. Here, we identify novel, volatile Orco antagonists. We functionally screened 83 structurally diverse compounds against Orco from Anopheles gambiae . Results were used for training ... subset of predicted active compounds revealed enrichment for Orco antagonists, many structurally distinct from previously known Orco ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2019 - Nature Portfolio | Scientific Reports
Anna Avesani, Valerio Marino, Serena Zanzoni, Karl‐Wilhelm Koch, Daniele Dell’Orco,
... cGMP phosphodiesterase 6 (PDE) (1Koch K.W. Dell'Orco D. Protein and signaling networks in vertebrate photoreceptor ... to shape their photoresponses (1Koch K.W. Dell'Orco D. Protein and signaling networks in vertebrate photoreceptor ... below the basal levels (1Koch K.W. Dell'Orco D. Protein and signaling networks in vertebrate photoreceptor ... Scopus (58) Google Scholar, 7Koch K.W. Dell'Orco D. A calcium-relay mechanism in vertebrate phototransduction. ... redundancy. GCAPs display different affinity for Ca2+ (12Dell'Orco D. Sulmann S. Linse S. Koch K.W. ...
Tópico(s): Ubiquitin and proteasome pathways
2021 - Elsevier BV | Journal of Biological Chemistry
Xiao-Bin Fan, Bao‐Tong Mo, Guo‐Cheng Li, Ling‐Qiao Huang, Hao Guo, Xin-Lin Gong, Chen‐Zhu Wang,
... heteromultimeric complex of the odorant receptor co-receptor (Orco) and a ligand-selective Or. Other types of ... also involved in the olfactory system of insects. Orco as an obligatory subunit of ORs is highly ... Results Herein, we successfully established a homozygous mutant ( Orco −/− ) of Helicoverpa armigera , a notorious crop pest, using ... olfactory response characteristics of wild type (WT) and Orco −/− adults and larvae. Orco −/− males were infertile, while Orco −/− females were fertile. The lifespan of Orco −/− females was longer than that of WT females. ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2022 - BioMed Central | BMC Biology
Hao Sun, Ling-Ao Bu, Shao-Cong Su, Di Guo, Cong‐Fen Gao, Shun‐Fan Wu,
... receptor neurons (ORNs), the odorant receptor co-receptor (Orco) is an obligatory component that is required for ... a ligand-gated ion channel complex. The ORs/Orco heteromeric complex plays a crucial role in insect ... applied CRISPR/Cas9 genome editing to mutate its Orco gene and constructed a homozygous mutant strain of Orco (Orco-/-) by genetic crosses. Electroantennogram (EAG) analysis showed that the responses of Orco-/- male moths to two universal sex pheromones, Z9- ... Z7-12: Ac, were abolished. We found that Orco-/- males cannot successfully mate with female moths. An ...
Tópico(s): Insect Resistance and Genetics
2022 - Elsevier BV | Insect Biochemistry and Molecular Biology
Patrick L. Jones, Gregory M. Pask, Ian M. Romaine, Robert W. Taylor, Paul R. Reid, Alex G. Waterson, Gary A. Sulikowski, Laurence J. Zwiebel,
... consisting of a well-conserved OR co-receptor (Orco) ion channel and a non-conserved tuning OR that provides coding specificity to each complex. Orco functions as a non-selective cation channel and ... of many insects are principally driven by olfaction, Orco represents a novel target for behavior-based control ... and synthetic odorants have been shown to agonize Orco/Or complexes, only a single direct Orco modulator, VUAA1, has been described. In an effort to identify additional Orco modulators, we have investigated the structure/activity relationships ...
Tópico(s): Insect Pheromone Research and Control
2012 - Public Library of Science | PLoS ONE
Latha Mukunda, Fabio Miazzi, Sabine Kaltofen, Bill S. Hansson, Dieter Wicher,
... protein (OrX) and a ubiquitous co-receptor protein (Orco). The ORs operate as non-selective cation channels, also conducting Ca(2+) ions. The Orco protein contains a conserved putative calmodulin (CaM)-binding ... antagonist W5. A similar effect was observed for Orco proteins heterologously expressed in CHO cells when CaM ... overexpression of CaM-EF-hand mutants. With the Orco CaM mutant bearing a point mutation in the ... were akin to those obtained for wild type Orco in the presence of W7. There was no ... responses in CHO cells expressing complete ORs (Or22a/Orco, Or47a/Orco, Or33a/Orco, Or56a/Orco). For Or33a ...
Tópico(s): Olfactory and Sensory Function Studies
2014 - Elsevier BV | Cell Calcium
Immo A. Hansen, Stacy D. Rodriguez, Lisa L. Drake, David Price, Brittny N. Blakely, John I. Hammond, Hitoshi Tsujimoto, Erika Y. Monroy, William A. Maio, Alvaro Romero,
... complexes of ORs and a universal OR coreceptor (Orco). Orco is the constant chain of every odorant receptor ... olfaction but does not directly bind to odorants. Orco agonists and antagonists have been suggested as high- ... and identified several odorant receptors as well as Orco. We characterized Orco expression and investigated the effect of chemicals targeting Orco on bed bug behavior and reproduction. We have ... OBPs and 16 ORs. Full length bed bug Orco was cloned and sequenced. Orco is widely expressed ...
Tópico(s): Insect and Pesticide Research
2014 - Public Library of Science | PLoS ONE
Andreas Nolte, Nico W. Funk, Latha Mukunda, Petra Gawalek, Achim Werckenthin, Bill S. Hansson, Dieter Wicher, Monika Stengl,
... membrane topology. They colocalize with a conserved coreceptor (Orco) with chaperone and ion channel function. Some studies ... insects employ exclusively ionotropic odor transduction via OR-Orco heteromers. Other studies provide evidence for different metabotropic ... transduction mechanism is employed. If indeed an OR-Orco ion channel complex underlies an ionotropic mechanism, then Orco agonist-dependent opening of the OR-Orco channel pore should add up to pheromone-dependent ... from intact pheromone-sensitive sensilla, perfusion with the Orco agonist VUAA1 did not increase pheromone-responses within ...
Tópico(s): Insect Pheromone Research and Control
2013 - Public Library of Science | PLoS ONE
Lorena Halty-deLeon, Fabio Miazzi, Sabine Kaltofen, Bill S. Hansson, Dieter Wicher,
... systems. We co-transfected the Drosophila olfactory coreceptor (Orco) in HEK293 cells with a truncated form of ... acetate and was shown to be apposed to Orco within the functional receptor unit. Co-transfection of Orco with either of the two constructs led to an enhanced response to stimulations with the synthetic Orco agonist VUAA1, as compared to transfection with Orco alone. This method enhances the functional expression of Orco in HEK293 cells in comparison to conventional transfection with Orco alone and enables the use of a lower ...
Tópico(s): Invertebrate Immune Response Mechanisms
2016 - Elsevier BV | Journal of Neuroscience Methods
Roshan Khadka, Colm Carraher, Cyril Hamiaux, Jadranka Travaš‐Sejdić, Andrew V. Kralicek,
... subunit, OrX, and an ion channel forming subunit, Orco. In our previous study, we showed that the ... we investigated the effect of the presence of Orco on the response of the OrX subunit to ... OrXs - Or10a, Or22a, Or35a and Or71a, together with Orco, were recombinantly expressed, purified and integrated into liposomes. These OrX/Orco liposomes were covalently attached to a gold surface ... acid (MHA). It was demonstrated that the OrX/Orco liposomes could sensitively and selectively detect their ligands ... a shift in their dose-response curves when Orco was co-integrated, reflecting an increase in ligand ...
Tópico(s): Insect Pheromone Research and Control
2020 - Elsevier BV | Biosensors and Bioelectronics
Ying Yang, Jürgen Krieger, Long Zhang, Heinz Breer,
... gregaria, which encode the olfactory receptor co-receptor (Orco). It was found that the sequences of LmigOrco ... of identity to each other and also to Orco proteins from different insect orders. The Orco-expressing cells in the antenna of S. gregaria ... L. migratoria were visualized by in situ hybridization. Orco expression could be assigned to clusters of cells ... trichodea, most likely representing olfactory sensory neurons. No Orco-positive cells were detected in sensilla coeloconica and sensilla chaetica. Orco expression was found already in all nymphal stages ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2012 - Ivyspring International Publisher | International Journal of Biological Sciences
Pablo F. German, S. van der Poel, Colm Carraher, Andrew V. Kralicek, Richard D. Newcomb,
... at least two subunit members: the non-canonical Orco ion channel subunit and a ligand-binding receptor ... interactions of the ligand-binding OR, Or22a, and Orco (Or22a–Or22a, Or22a–Orco, Orco–Orco). There was no evidence for an interaction between SNMP1 and Orco or between SNMP2 and Orco or Or22a. However, fusion proteins of SNMP1 and ... supports previously observed homomeric and heteromeric interactions between Orco and the ligand-binding OR, Or22a, and identifies ...
Tópico(s): Plant biochemistry and biosynthesis
2012 - Elsevier BV | Insect Biochemistry and Molecular Biology
Ramgopal Nadimpalli, Nasser Yalpani, Gurmukh S. Johal, Carl R. Simmons,
... Ref. 7McClung J.K. Jupe E.R. Dell'Orco R.T. Exp. Gerontol. 1995; 30: 99-124Crossref ... A. Smith J.R. Schneider E.L. Dell'Orco R. Lumpkin C.K. Danner D.B. McClung ... cycle (7McClung J.K. Jupe E.R. Dell'Orco R.T. Exp. Gerontol. 1995; 30: 99-124Crossref ... A. Smith J.R. Schneider E.L. Dell'Orco R. Lumpkin C.K. Danner D.B. McClung ... control (7McClung J.K. Jupe E.R. Dell'Orco R.T. Exp. Gerontol. 1995; 30: 99-124Crossref ... membrane (7McClung J.K. Jupe E.R. Dell'Orco R.T. Exp. Gerontol. 1995; 30: 99-124Crossref ... membranes (7McClung J.K. Jupe E.R. Dell'Orco R.T. Exp. Gerontol. 1995; 30: 99-124Crossref ...
Tópico(s): Plant Virus Research Studies
2000 - Elsevier BV | Journal of Biological Chemistry
Andreas Nolte, Petra Gawalek, Sarah Koerte, HongYing Wei, Robin S. Schumann, Achim Werckenthin, Jürgen Krieger, Monika Stengl,
... topology. They associate with the conserved ion channel Orco. As chaperon, Orco maintains ORs in cilia and, as pacemaker channel, Orco controls spontaneous activity in olfactory receptor neurons. Odorant binding to ORs opens OR-Orco receptor ion channel complexes in heterologous expression systems. ... Because it is still unclear how and when Orco opens after odor-OR-binding, we used tip ... to examine in vivo the effects of the Orco antagonist OLC15 and the amilorides MIA and HMA ... Zeitgebertime-dependently. Our results suggest no involvement for Orco in the primary transduction events, in contrast to ...
Tópico(s): Insect and Arachnid Ecology and Behavior
2016 - Public Library of Science | PLoS ONE
Konstantina T. Tsoumani, Alexandros Belavilas-Trovas, Maria‐Eleni Gregoriou, Kostas D. Mathiopoulos,
... gene that encodes the conserved olfactory co-receptor Orco (Odorant receptor co-receptor), which interacts with all ... We initially searched the expression profile of Bo-Orco in both sexes during sexual maturation, as well ... time PCR (qRT-PCR) analysis suggesting that Bo-Orco was abundantly expressed in sexually mature adults. We further investigated the functional role of Bo-Orco in mating and oviposition behavior via transient gene ... in sexually mature flies 7 days after eclosion. Orco -knockdown phenotypes in both sexes showed reduced copulation ...
Tópico(s): Insect Utilization and Effects
2020 - BioMed Central | BMC Genomic Data
Zhenqing Chen, Ian M. Traniello, Seema Rana, Amy Cash-Ahmed, Alison L. Sankey, Yang Che, Gene E. Robinson,
... functionality of the highly conserved co-receptor gene orco. Genome editing studies in a few species of ants and moths have revealed that orco can also have a neurodevelopmental function, in addition ... melanogaster. To extend this analysis, we determined whether orco mutations also affect the development of the adult ... communication. We used CRISPR/Cas9 to knock out orco and examined anatomical and molecular consequences. To increase ... Sanger sequencing to quickly select individuals with complete orco knockout for neuroanatomical analyses and later validated and ...
Tópico(s): Insect and Pesticide Research
2021 - Taylor & Francis | Journal of Neurogenetics
Vardanush Sargsyan, Merid N. Getahun, Sofía Lavista Llanos, Shannon B. Olsson, Bill S. Hansson, Dieter Wicher,
... an olfactory receptor protein and the ion channel Orco. Heterologously expressed insect ORs are activated via an ... that leads to cAMP production and activates the Orco channel. The contribution of metabotropic signaling to the ... investigated this phenomenon in HEK293 cells expressing Drosophila Orco and found that phospholipase C (PLC) inhibition reduced the sensitivity of Orco to cAMP. A similar effect was seen upon ... protein kinase C (PKC), whereas PKC stimulation activated Orco even in the absence of cAMP. Mutation of ...
Tópico(s): Olfactory and Sensory Function Studies
2011 - Frontiers Media | Frontiers in Cellular Neuroscience
... common subunit (the odorant receptor co-receptor subunit, Orco) and at least one variable odorant specificity subunit. ... shown to act directly on the highly conserved Orco subunit, suggesting that broadly active compounds can be ... the VUAA1 structure in order to identify new Orco ligands. Principal Findings We screened ORs from several ... also yielded competitive antagonists. Importantly, we show that Orco antagonists inhibit odorant activation of ORs from several ... pattern of agonist and antagonist sensitivity displayed by Orco subunits from different species suggests a highly conserved ...
Tópico(s): Insect Utilization and Effects
2012 - Public Library of Science | PLoS ONE