A Complete View of Golden Hall in the Centre of the Shitennoji Temple Buildings. To the Left Rear Is Part of the Corridor, and the Hall Connected to the ...
0000 - Gale Group | NCCO Photography
Tsutomu Nakazawa, Mitsutoshi Nakamura, Young-Soo Park, Yasushi Motoyama, Yasuo Hironaka, Fumihiko Nishimura, Ichiro Nakagawa, Shuichi Yamada, Ryosuke Matsuda, Kentaro Tamura, Tadashi Sugimoto, Yasuhiro Takeshima, Akiko Marutani, Takahiro Tsujimura, Noriko Ouji, Yukiteru Ouji, Masahide Yoshikawa, Hiroyuki Nakase,
Glioblastoma (GBM) is a highly aggressive brain tumor for which novel therapeutic approaches, such as immunotherapy, are urgently needed. Zoledronate (ZOL), an inhibitor of osteoclastic activity, is known to stimulate peripheral blood-derived γδT cells and sensitize tumors to γδT cell-mediated killing. To investigate the feasibility of γδT cell-based immunotherapy for patients with GBM, we focused on the killing of GBM cell lines by γδT cells and the molecular mechanisms involved in these cell–cell ...
Tópico(s): Glioma Diagnosis and Treatment
2013 - Springer Science+Business Media | Journal of Neuro-Oncology
The Bell Tower of Rinoji Temple Was Moved from Toshogu to a Spot Southwest of Sanbutsudo in 1882. The Tower Had Three Pillars on Each Corner, or a Total ...
0000 - Gale Group | NCCO Photography
Yukiteru Ouji, Masahide Yoshikawa, Mariko Nishiofuku, Noriko Ouji-Sageshima, Atsushi Kubo, Shigeaki Ishizaka,
Although mouse Wnt-10b has been shown to play various roles in a wide range of biological actions, the effects on epithelial stem/progenitor cells in the skin have not been reported. In the present study, we investigated the effects of Wnt-10b on proliferation and differentiation of murine skin-derived CD34 and CD49f double-positive (CD34+CD49f+) cells, a supposed fraction as enriched epithelial stem/progenitor cells. The cells were prepared from dorsal skin samples obtained from young adult mice ...
Tópico(s): Skin and Cellular Biology Research
2010 - Elsevier BV | Journal of Bioscience and Bioengineering
The Garden of Ogiya Restaurant Is on the Left. The Water Is Flowing through Grooves in the Rocks Apparently Made by Long Exposure. Felice Beato Took a ...
0000 - Gale Group | NCCO Photography
Hideshi Kobayashi, Kazuhiko Tsuneki, Hideo Akiyoshi, Yuta Kobayashi, Masumi Nozaki, Masami Ouji,
The histochemical distribution of peroxidase was studied in 10 species of ascidians. In the endostyle, strong peroxidase activity was found in zone 7 in Ciona intestinalis, Ascidia zara, Ascidia sydneiensis samea, Cnemidocarpa areolata, Styela clava, and Pyrura vittata. The activity in zone 7 was weak in Styela plicata and Halocynthia hilgendorfi. Pyura michaelseni and Halocynthia roretzi showed only faint activity in zone 7, but showed strong activity in zone 9 and in the transitional zone, respectively. ...
Tópico(s): Marine Biology and Environmental Chemistry
1983 - Elsevier BV | General and Comparative Endocrinology
One of Two Gold Screens at Rinoji Temple. This Screen Was Ordered for the 21st Anniversary of the Death of the Shogun Tokugawa Ieyasu. 72 Dancers Are ...
0000 - Gale Group | NCCO Photography
Kazuhiko Tsuneki, Hideshi Kobayashi, Masami Ouji,
The histochemical distribution of peroxidase was studied in amphioxus, ammocoetes larvae, adult lampreys, and hagfish. The endostyle of amphioxus displayed peroxidase activity in zone 5 and, in some individuals, in zone 1 as well. The endostyle of ammocoetes exhibited strong peroxidase activity in type 2c and type 3 cells. These peroxidase-positive regions coincide well with radioiodine-binding regions previously described. Thyroid follicles of adult lampreys stained strongly for peroxidase, but ...
Tópico(s): Aquaculture disease management and microbiota
1983 - Elsevier BV | General and Comparative Endocrinology
Four Men in Armor Are Standing in Front of the Golden Screen at Rinoji Temple. The Bugaku Costumes Were Dedicated to the Temple Together with the Screen ...
0000 - Gale Group | NCCO Photography
Hayato Toriumi, Masahide Yoshikawa, Ryosuke Matsuda, Fumihiko Nishimura, Shuichi Yamada, Hidehiro Hirabayashi, Hiroyuki Nakase, Jun-Ichi Nonaka, Yukiteru Ouji, Shigeaki Ishizaka, Toshisuke Sakaki,
Abstract Objective: The purpose of the present study was to examine the efficacy of transplantation of mouse embryonic stem (ES) into Parkinson's disease (PD) model mice as well as the necessity of immunosuppression in allogeneic donor-host combinations. Materials and methods: ES cells, derived from SvJ129 strain mice, were differentiated into tyrosine hydroxylase (TH)-positive neurons in vitro by an embryoid body (EB)-based multistep differentiation method and used as graft cells for PD mice, which ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2009 - Taylor & Francis | Neurological Research
... Prefecture) and Thus Established a Shrine to Jakuichi Ouji Gongen (Kumano Gongen) That Later Became Oji Gongen. ...
0000 - Gale Group | NCCO Photography
Yann Leydier, Asma Ouji, Frank Lebourgeois, Hubert Emptoz,
In this article, we introduce the first method that allows the indexation of ancient manuscripts of any language and alphabet. We describe a word retrieval engine inspired by recent word-spotting advances on ancient manuscripts. Our approach does not need any layout segmentation and makes use of features fitted to any type of alphabet (Latin, Arabic, Chinese, etc.) and writing. The engine is tested on numerous documents and in several use-cases.
Tópico(s): Video Analysis and Summarization
2009 - Elsevier BV | Pattern Recognition
Keiji Shimada, Mitsutoshi Nakamura, Marco A. De Velasco, Motoyoshi Tanaka, Yukiteru Ouji, Makito Miyake, Kiyohide Fujimoto, Kazuya Hirao, Noboru Konishi,
Heparan sulfate proteoglycan syndecan-1, CD138, is well known to be associated with cell proliferation, adhesion, and migration in various types of malignancies. In the present study, we focused on the role of syndecan-1 in human urothelial carcinoma of the urinary bladder. Silencing of syndecan-1 by siRNA transfection down-regulated transcriptional factor junB and the long isoform of FLICE-inhibitory protein (FLIP long), resulting in the induction of apoptosis in the urothelial carcinoma cell lines ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2009 - Wiley | Cancer Science
Keiji Shimada, Mitsutoshi Nakamura, Marco A. De Velasco, Motoyoshi Tanaka, Yukiteru Ouji, Noboru Konishi,
Heparan sulfate proteoglycan syndecan-1 (CD138) is well known to be associated with cell proliferation, adhesion and migration in various types of malignancies. In the present study, we focused on the role of syndecan-1 in human prostate cancer. Immunohistochemical analysis revealed either no or rare expression of syndecan-1 in normal secretory glands and prostate cancer cells at hormone naïve status, whereas the expression was significantly increased in viable cancer cells following neo-adjuvant ...
Tópico(s): Protease and Inhibitor Mechanisms
2009 - Wiley | Cancer Science
Ryosuke Matsuda, Masahide Yoshikawa, Hajime Kimura, Yukiteru Ouji, Hiroyuki Nakase, Fumihiko Nishimura, Jun-Ichi Nonaka, Hayato Toriumi, Shuichi Yamada, Mariko Nishiofuku, Kei Moriya, Shigeaki Ishizaka, Mitsutoshi Nakamura, Toshisuke Sakaki,
Embryonic stem (ES) cells are a potential source for treatment of spinal cord injury (SCI). Although one of the main problems of ES cell-based cell therapy is tumor formation, there is no ideal method to suppress tumor development. In this study, we examined whether transplantation with bone marrow stromal cells (BMSCs) prevented tumor formation in SCI model mice that received ES cell-derived grafts containing both undifferentiated ES cells and neural stem cells. Embryoid bodies (EBs) formed in 4-day ...
Tópico(s): Mesenchymal stem cell research
2009 - SAGE Publishing | Cell Transplantation
Keiji Shimada, Mitsutoshi Nakamura, Satoshi Anai, Marco A. De Velasco, Motoyoshi Tanaka, Kazutake Tsujikawa, Yukiteru Ouji, Noboru Konishi,
Abstract We recently identified a novel human AlkB homologue, ALKBH8, which is expressed in various types of human cancers including human urothelial carcinomas. In examining the role and function of ALKBH8 in human bladder cancer development in vitro, we found that silencing of ALKBH8 through small interfering RNA transfection reduced reactive oxygen species (ROS) production via down-regulation of NAD(P)H oxidase-1 (NOX-1) and induced apoptosis through subsequent activation of c-jun NH2-terminal ...
Tópico(s): Folate and B Vitamins Research
2009 - American Association for Cancer Research | Cancer Research
Fumihiko Nishimura, Masahide Yoshikawa, Seiji Kanda, Masahiro Nonaka, Hiroshi Yokota, Akira Shiroi, Hiroyuki Nakase, Hidehiro Hirabayashi, Yukiteru Ouji, Jun‐ichi Birumachi, Shigeaki Ishizaka, Toshisuke Sakaki,
Abstract Background and Aims. The purpose of the present study was to examine the efficacy of transplantation of mouse embryonic-stem-(ES)-cell-derived tyrosine hydroxylase-positive (TH+) cells into Parkinsonian mice using behavioral tests and immunohistochemical evaluation. Methods. Undifferentiated ES cells carrying the enhanced green fluorescent protein (EGFP) gene were differentiated into a cell population containing TH+ neurons using a five-step in vitro differentiation method. These ES-cell- ...
Tópico(s): Nerve injury and regeneration
2003 - Oxford University Press | Stem Cells
Yukiteru Ouji, Masahide Yoshikawa, Kei Moriya, Mariko Nishiofuku, Ryosuke Matsuda, Shigeaki Ishizaka,
Although Wnts are expressed in hair follicles throughout life from embryo to adult, and considered to be critical for their development and maturation, their roles remain largely unknown. In the present study, we investigated the effects of Wnts (Wnt-3a, Wnt-5a, Wnt-10b, and Wnt-11) on epithelial cell differentiation using adult mouse-derived primary skin epithelial cell (MPSEC) cultures and hair growth using hair follicle organ cultures. Only Wnt-10b showed evident promotion of epithelial cell differentiation ...
Tópico(s): Nerve injury and regeneration
2008 - Elsevier BV | Biochemical and Biophysical Research Communications
Masahide Yoshikawa, Mariko Nishiofuku, Kei Moriya, Yukiteru Ouji, Shigeaki Ishizaka, Kei Kasahara, Keiichi Mikasa, Toshiko Hirai, Youka Mizuno, Shuhei Ogawa, Takahito Nakamura, Haruhiko Maruyama, Nobuaki Akao,
We present 3 adult cases of visceral toxocariasis from the same family, who each consumed thin slices of raw bovine liver weekly, and developed eosinophilia and multiple small lesions in their livers and lungs. Serological examinations using the larval excretory–secretory product of Toxocara canis strongly indicated infection with Toxocara species larvae. The patients responded well to treatment with albendazole. Ingestion of raw liver from paratenic animals is considered to be a common transmission ...
Tópico(s): Dermatological diseases and infestations
2008 - Elsevier BV | Parasitology International
Kei Moriya, Masahide Yoshikawa, Yukiteru Ouji, Ko Saito, Mariko Nishiofuku, Ryosuke Matsuda, Shigeaki Ishizaka, Hiroshi Fukui,
Summary We transplanted undifferentiated embryonic stem (ES) cells into the spleens of carbon tetrachloride (CCl 4 )‐treated mice to determine their effects on liver fibrosis. Carbon tetrachloride at 0.5 ml/kg of body weight was injected intraperitoneally into C57BL/6 mice twice weekly for up to 20 weeks. Four weeks after the first injection, the mice were divided into two groups and those in group 1 received 1 × 10 5 ES cells genetically labelled with enhanced green fluorescent protein (GFP) in the spleens, ...
Tópico(s): 3D Printing in Biomedical Research
2008 - Wiley | International Journal of Experimental Pathology
Yukiteru Ouji, Masahide Yoshikawa, Kei Moriya, Shigeaki Ishizaka,
Wnts are deeply involved in the proliferation and differentiation of skin epithelial cells. We previously reported the differentiation of cultured primary skin epithelial cells toward hair shaft and inner root sheath (IRS) of the hair follicle via β-catenin stabilization caused by Wnt-10b, however, the effects of Wnt-10b on cultured hair follicles have not been reported. In the present study, we examined the effects of Wnt-10b on shaft growth using organ cultures of whisker hair follicles in serum- ...
Tópico(s): Skin and Cellular Biology Research
2007 - Elsevier BV | Biochemical and Biophysical Research Communications
Ali Ouji, María José Suso, Mustapha Rouaissi, Raoudha Abdellaoui, Mohamed El Gazzah,
Tópico(s): Genetic diversity and population structure
2011 - Springer Science+Business Media | Genes & Genomics
Di Huang, Karima Ouji, Mohsen Ardabilian, Yunhong Wang, Liming Chen,
In recent years, 3D face recognition has been considered as a major solution to deal with these unsolved issues of reliable 2D face recognition, i.e. illumination and pose variations. This paper focuses on two critical aspects of 3D face recognition: facial feature description and classifier design. To address the former one, a novel local descriptor, namely Local Shape Patterns (LSP), is proposed. Since LSP operator extracts both differential structure and orientation information, it can describe ...
Tópico(s): Image Retrieval and Classification Techniques
2011 - Springer Science+Business Media | Lecture notes in computer science
Yukiteru Ouji, Masahide Yoshikawa, Akira Shiroi, Shigeaki Ishizaka,
To evaluate the role of Wnt-10b in epithelial differentiation, we investigated the effects of Wnt-10b on adult mouse-derived primary skin epithelial cells (MPSEC). Recombinant Wnt-10b protein (rWnt-10b) was prepared using a gene engineering technique and MPSEC were cultured in its presence, which resulted in morphological changes from cuboidal to spindle-shaped and inhibited their proliferation. Further, involvement of the canonical Wnt signal pathway was also observed. MPSEC treated with rWnt-10b ...
Tópico(s): Skin and Cellular Biology Research
2006 - Elsevier BV | Biochemical and Biophysical Research Communications
Yukiteru Ouji, Masahide Yoshikawa, Akira Shiroi, Shigeaki Ishizaka,
We previously showed that Wnt-10b promoted the differentiation of primary skin epithelial cells (MPSEC) toward hair shaft and inner root sheath of the hair follicle (IRS) cells in vitro. In the present study, we found that Wnt-10b promotes the development of hair follicles using a culture of mouse embryonic skin tissue and trichogenesis using a reconstitution experiment with nude mice. Hair follicle development was observed in skin taken from mouse embryos on embryonic day 10.5 following a 2-day culture ...
Tópico(s): Skin and Cellular Biology Research
2006 - Elsevier BV | Biochemical and Biophysical Research Communications
Yukiteru Ouji, Masahide Yoshikawa, Akira Shiroi, Shigeaki Ishizaka,
Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions, while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and ...
Tópico(s): Skin and Cellular Biology Research
2006 - Elsevier BV | Biochemical and Biophysical Research Communications
Takashi Uchida, Ouji Ohashi, H. Kawamoto, Hirofumi Yoshimura, Ken-ichi KOBAYASHI, Makoto Tanimura, Naohiro Fujikawa, Tetsuro Nishimoto, Kazuhiko Awata, Masaru Tachibana, Kenichi Kojima,
We show that diesel soot can be recycled as a carbon source for the synthesis of single-wall carbon nanotubes (SWNTs). The synthesis of SWNTs was carried out by the laser vaporization of diesel soot. The presence of SWNTs was confirmed by Raman spectroscopy and transmission electron microscopy. SWNTs produced in this way should provide economic benefits and also contribute to a cleaner environment.
Tópico(s): Fullerene Chemistry and Applications
2006 - Institute of Physics | Japanese Journal of Applied Physics
Yukiteru Ouji, Shigeaki Ishizaka, Masahide Yoshikawa,
Dermal papilla (DP) cells are associated with the development of hair follicles (HFs) and regulation of the hair cycle. However, primary DP cells prepared from cultured HFs are known to lose their ability to induce HF after culturing in standard media, for example, fibroblast growth conditions. We explored a new culture condition by which DP cells maintained their HF induction ability. The addition of Wnt-10b to the first culture of primary DP cells promoted their proliferation and maintained their ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2012 - SAGE Publishing | Cell Transplantation
Yukiteru Ouji, Shinji Ishizaka, Fukumi Nakamura‐Uchiyama, Masahiro Yoshikawa,
Hearing loss is mainly caused by loss of sensory hair cells (HCs) in the organ of Corti or cochlea. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about the efficient generation of HC-like cells from ES cells. In the present study, we developed a single-medium culture method for growing embryoid bodies (EBs), in which conditioned medium (CM) from cultures of ST2 stromal cells (ST2-CM) was used for 14-day cultures of 4-day EBs. At the end of the 14-day cultures, ...
Tópico(s): Pluripotent Stem Cells Research
2012 - Springer Nature | Cell Death and Disease
Rie Ommori, Noriko Ouji, Fumiko Mizuno, Eiji Kita, Yoshito Ikada, Hideo Asada,
Staphylococcus aureus (S. aureus) is one of the most clinically important inflammation-inducing pathogens, while Staphylococcus epidermidis (S. epidermidis) is nonpathogenic and hardly causes inflammation on skin. β-defensins, antimicrobial peptides, are secreted from keratinocytes constitutively or upon induction by various microorganisms. However, the difference between S. aureus and S. epidermidis is still unclear in terms of their influences on the production of β-defensins. In this study, we ...
Tópico(s): Immune Response and Inflammation
2012 - Elsevier BV | Microbial Pathogenesis