Y. Sasaki, Takashi Ideue, Miho Sano, Toutai Mituyama, Tetsuro Hirose,
... MEN) I locus and which localize to nuclear paraspeckles. This finding raises the intriguing possibility that MENepsilon/beta are involved in paraspeckle organization, because paraspeckles are, reportedly, RNase-sensitive structures. Successful removal of ... beta by a refined knockdown method resulted in paraspeckle disintegration. Furthermore, the reassembly of paraspeckles disassembled by transcriptional arrest appeared to be unsuccessful ... RNA interference and immunoprecipitation further revealed that the paraspeckle proteins p54/nrb and PSF selectively associate with ...
Tópico(s): RNA modifications and cancer
2009 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Christine Clemson, John N. Hutchinson, Sergio Andres Sara, Alexander W. Ensminger, Archa H. Fox, Andrew Chess, Jeanne B. Lawrence,
... foci that we show are completely coincident with paraspeckles, nuclear domains implicated in mRNA nuclear retention. Depletion of NEAT1 RNA via RNAi eradicates paraspeckles, suggesting that it controls sequestration of the paraspeckle proteins PSP1 and p54, factors linked to A- ... editing. Unlike overexpression of PSP1, NEAT1 overexpression increases paraspeckle number, and paraspeckles emanate exclusively from the NEAT1 transcription site. The ... required for its colocalization with NEAT1 RNA in paraspeckles, and biochemical analyses support that NEAT1 RNA binds ...
Tópico(s): RNA and protein synthesis mechanisms
2009 - Elsevier BV | Molecular Cell
... ncRNA that is an integral component of nuclear paraspeckles. Paraspeckles are discrete structures that typically localize adjacent to ... to provide the crucial structural framework to nucleate paraspeckle formation. The precedent for a class of architectural ... ncRNAs was thus established. Although the function of paraspeckles is not wholly understood, the identity of associated ... p54nrb, PSF, and PSP1) are characteristically enriched in paraspeckles, and NEAT1 is required for their localization (Chen ... 900) Google Scholar). The presence of p54nrb in paraspeckles strongly suggests that they are involved in retention ...
Tópico(s): RNA modifications and cancer
2009 - Elsevier BV | Molecular Cell
Yuntao S. Mao, Hongjae Sunwoo, Bin Zhang, David L. Spector,
The formation of subnuclear domains, such as the paraspeckles where hyperdited mRNAs are stored, is not well understood. The transcription of the paraspeckle non-coding RNA MEN ɛ/β initiates the ... molecular mechanism of how one nuclear body, the paraspeckle, is assembled and organized. Paraspeckles are discrete ribonucleoprotein bodies found in mammalian cells ... and the direct visualization of the recruitment of paraspeckle proteins. Using this system, we demonstrate that Men ... essential to initiate the de novo assembly of paraspeckles. These newly formed structures effectively harbour nuclear-retained ...
Tópico(s): Genomics and Chromatin Dynamics
2010 - Nature Portfolio | Nature Cell Biology
Takao Naganuma, Shinichi Nakagawa, Akie Tanigawa, Y. Sasaki, Naoki Goshima, Tetsuro Hirose,
... of long noncoding RNA initiates construction of nuclear paraspeckles Takao Naganuma Takao Naganuma Functional RNomics Team, Biomedicinal ... to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Paraspeckles are unique subnuclear structures built around a specific ... address the precise molecular processes that lead to paraspeckle formation, we identified 35 paraspeckle proteins (PSPs), mainly by colocalization screening with a ... Subsequent RNAi analyses identified seven essential PSPs for paraspeckle formation. One of the essential PSPs, HNRNPK, appeared ...
Tópico(s): RNA modifications and cancer
2012 - Springer Nature | The EMBO Journal
Tetsuro Hirose, Shinichi Nakagawa,
... long ncRNA functions that remain largely enigmatic. A paraspeckle is a nuclear body that is almost ubiquitously ... cell population-specific in adult mouse tissue. The paraspeckle structure is RNase-sensitive. Long ncRNAs, termed MENε/ ... been identified as the RNA components of the paraspeckles. Specific elimination has revealed that MENε/β ncRNAs ... essential components for the formation of the intact paraspeckle structure. Paraspeckle formation requires the continual MENε/β ncRNA biogenesis ... transcription, alternative 3'-end processing, and stabilization. Some paraspeckle-localized RNA-binding proteins (p54/nrb and PSF) ...
Tópico(s): RNA and protein synthesis mechanisms
2012 - De Gruyter | BioMolecular Concepts
Wen Shen, Xue‐hai Liang, Stanley T. Crooke,
Nuclear paraspeckles are built co-transcriptionally around a long non-coding RNA, NEAT1. Here we report that transfected 20- ... phosphorothioate-modified (PS) antisense oligonucleotides (ASOs) can recruit paraspeckle proteins to form morphologically normal and apparently functional paraspeckle-like structures containing no NEAT1 RNA. PS-ASOs can associate with paraspeckle proteins, including P54nrb, PSF, PSPC1 and hnRNPK. NEAT1 ... can be displaced by transfected PS-ASO from paraspeckles and rapidly degraded. Co-localization of PS-ASOs ...
Tópico(s): RNA modifications and cancer
2014 - Oxford University Press | Nucleic Acids Research
Tetsuro Hirose, Giorgio Virnicchi, Akie Tanigawa, Takao Naganuma, Ruohan Li, Hiroshi Kimurâ, Takahide Yokoi, Shinichi Nakagawa, Marianne Bénard, Archa H. Fox, Gérard Pierron,
Paraspeckles are subnuclear structures formed around nuclear paraspeckle assembly transcript 1 (NEAT1)/MENε/β long noncoding RNA (lncRNA). Here we show that paraspeckles become dramatically enlarged after proteasome inhibition. This enlargement ... transcriptional up-regulation rather than accumulation of undegraded paraspeckle proteins. Of interest, however, using immuno-electron microscopy, we find that key paraspeckle proteins become effectively depleted from the nucleoplasm by 50% when paraspeckle assembly is enhanced, suggesting a sequestration mechanism. We ...
Tópico(s): RNA Research and Splicing
2013 - American Society for Cell Biology | Molecular Biology of the Cell
Tatyana A. Shelkovnikova, Hannah K. Robinson, Claire Troakes, Natalia Ninkina, Vladimir L. Buchman,
Paraspeckles are nuclear bodies formed by a set of specialized proteins assembled on the long non-coding ... a number of neurodegenerative disorders, is an essential paraspeckle component. We have shown that its recruitment to ... interacts with p54nrb/NONO, a major constituent of paraspeckles, in an RNA-dependent manner and responds in the same way as other paraspeckle proteins to alterations in cellular homeostasis such as ... protein methylation. FUS also regulates NEAT1 levels and paraspeckle formation in cultured cells, and FUS deficiency leads ...
Tópico(s): RNA Research and Splicing
2013 - Oxford University Press | Human Molecular Genetics
Takao Naganuma, Tetsuro Hirose,
Paraspeckles are unique subnuclear structures that are built around a specific long non-coding RNA (lncRNA), NEAT1, ... P. NEAT1_2 is an essential component for paraspeckle formation. Paraspeckles form during the NEAT1_2 lncRNA biogenesis process, ... processing and accumulation. Recent RNAi analyses of 40 paraspeckle proteins (PSPs) identified four PSPs that are required for paraspeckle formation by mediating NEAT1 processing and accumulation. In ... synthesis. The other three PSPs were required for paraspeckle formation, but did not affect NEAT1_2 expression. ...
Tópico(s): RNA regulation and disease
2013 - Taylor & Francis | RNA Biology
Tetsuya Kawaguchi, Akie Tanigawa, Takao Naganuma, Yasuyuki Ohkawa, Sylvie Souquère, Gérard Pierron, Tetsuro Hirose,
Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved ... stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 ... biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/ ... SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. ...
Tópico(s): interferon and immune responses
2015 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Aparna Anantharaman, Mahdieh Jadaliha, Vidisha Tripathi, Shinichi Nakagawa, Tetsuro Hirose, Michael F. Jantsch, Supriya G. Prasanth, Kannanganattu V. Prasanth,
Paraspeckles are sub-nuclear domains that are nucleated by long noncoding RNA Neat1. While interaction of protein components of paraspeckles and Neat1 is understood, there is limited information ... the interaction of non-structural RNA components with paraspeckles. Here, by varying paraspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their non-structural ... remains nuclear-retained in the absence of intact paraspeckles, suggesting that they do not regulate nuclear retention ...
Tópico(s): RNA Research and Splicing
2016 - Nature Portfolio | Scientific Reports
Archa H. Fox, Shinichi Nakagawa, Tetsuro Hirose, Charles S. Bond,
Paraspeckles have become an important model system for studying long noncoding RNA–protein interactions in the context ... recruit nuclear RNA-binding proteins and build a paraspeckle. Protein domains that mediate liquid–liquid phase separation are essential for many aspects of paraspeckle formation, including gluing together individual ribonucleoprotein bundles into a mature paraspeckle. Paraspeckle formation is dynamic and triggered by many different ... RNA and protein components. One such system is paraspeckles – protein-rich nuclear organelles built around a specific ...
Tópico(s): RNA regulation and disease
2017 - Elsevier BV | Trends in Biochemical Sciences
Shuai Wang, Qian Zhang, Qinlan Wang, Qicong Shen, Xiang Chen, Zhenyang Li, Ye Zhou, Jin Hou, Bo‐Wen Xu, Nan Li, Xuetao Cao,
ABSTRACT The formation of paraspeckle, a stress-induced nuclear body, increases in response to viral infection or proinflammatory stimuli. Paraspeckle consists of lncRNA (nuclear paraspeckle assembly transcript 1, NEAT1) and protein components including ... according to TCGA data. However, the role of paraspeckle in HCC progression needs further identification. IL-6 ... Here we reported that IL-6 signaling increased paraspeckle formation in HCC cells. Destruction of paraspeckle formation by silencing the paraspeckle essential components NEAT1_ ...
Tópico(s): RNA modifications and cancer
2018 - Landes Bioscience | OncoImmunology
Tatyana A. Shelkovnikova, Michail S. Kukharsky, Haiyan An, Pasquale Dimasi, Svetlana Alexeeva, Osman Shabir, Paul R. Heath, Vladimir L. Buchman,
Paraspeckles are subnuclear bodies assembled on a long non-coding RNA (lncRNA) NEAT1. Their enhanced formation in ... whether and how TDP-43 pathology may augment paraspeckle assembly. Paraspeckle formation in human samples was analysed by RNA- ... and protein overexpression. We show that de novo paraspeckle assembly in spinal neurons and glial cells is ... but not its cytoplasmic accumulation or aggregation augments paraspeckle assembly in cultured cells. TDP-43 is a component of the microRNA machinery, and recently, paraspeckles have been shown to regulate pri-miRNA processing. ...
Tópico(s): Cancer-related gene regulation
2018 - BioMed Central | Molecular Neurodegeneration
Haiyan An, Lucy Skelt, Antonietta Notaro, J. Robin Highley, Archa H. Fox, Vincenzo La Bella, Vladimir L. Buchman, Tatyana A. Shelkovnikova,
... less understood. FUS is an essential component of paraspeckles, subnuclear bodies assembled on a lncRNA NEAT1. Paraspeckles may play a protective role specifically in degenerating ... endogenous levels of mutant FUS would affect NEAT1/paraspeckles. Using novel cell lines with the FUS gene ... mutant FUS cause accumulation of NEAT1 isoforms and paraspeckles. However, despite only mild cytoplasmic mislocalisation of FUS, paraspeckle integrity is compromised in these cells, as confirmed by reduced interaction of mutant FUS with core paraspeckle proteins NONO and SFPQ and increased NEAT1 extractability. ...
Tópico(s): RNA Research and Splicing
2019 - Springer Science+Business Media | Acta Neuropathologica Communications
Paraspeckles are a type of subnuclear bodies built on the long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1, also known as MEN-ε/β or VINC-1). Paraspeckles are involved in many physiological processes including cellular ... ultra-resolution microscopy coupled with multicolor-labeling of paraspeckle components (the NEAT1 RNA and paraspeckle proteins) revealed the exquisite details of paraspeckle structure and function. NEAT1 transcripts are radially arranged to form a core-shell spheroidal structure, while paraspeckle proteins (PSPs) localize within different layers. Functional dissection ...
Tópico(s): Cancer-related molecular mechanisms research
2020 - Portland Press | Essays in Biochemistry
Tomohiro Yamazaki, Tetsuya Yamamoto, Hyura Yoshino, Sylvie Souquère, Shinichi Nakagawa, Gérard Pierron, Tetsuro Hirose,
Article22 April 2021free access Source DataTransparent process Paraspeckles are constructed as block copolymer micelles Tomohiro Yamazaki Corresponding Author Tomohiro Yamazaki [email protected] orcid.org/0000-0003-0866-5173 Graduate School of ... favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Paraspeckles are constructed by NEAT1_2 architectural long noncoding ... theoretically investigated how the shape and organization of paraspeckles are determined. We identified the NEAT1_2 RNA ...
Tópico(s): Photosynthetic Processes and Mechanisms
2021 - Springer Nature | The EMBO Journal
Jie Zan, Xuya Zhao, Xiya Deng, Hongda Ding, Wang Bi, Minyi Lu, Zi-Jing Wei, Zhi Huang, Shuai Wang,
... malignancies, with poor prognosis and low survival rate. Paraspeckles, which are unique subnuclear structures, are recently found ... This study aimed to investigate the possibility of paraspeckle in HCC cells participating in immune escape and ... vivo.MethodsExpression of NEAT1_2, the framework of paraspeckle, in HCC cells and tissues was detected by ... CRISPR-CAS9 knock-in technology. The effects of paraspeckle on HCC sensitivity to T-cell-mediated cytolysis ... immunoprecipitation, Western blotting, and ELISA. The role of paraspeckle during adoptive T-cell transfer therapy for HCC ...
Tópico(s): Immune Cell Function and Interaction
2021 - Elsevier BV | Cellular and Molecular Gastroenterology and Hepatology
Pei Wen Lee, A.C. Marshall, Gavin J. Knott, Simon Kobelke, Luciano G. Martelotto, Hyun‐Jung Cho, Paul J. McMillan, Mihwa Lee, Charles S. Bond, Archa H. Fox,
... their recruitment to distinct subnuclear locations, for example, paraspeckle condensates, where DBHS proteins bind to the long ... RNA NEAT1 in the first essential step in paraspeckle formation. To carry out their diverse roles, DBHS ... for live-cell 3D-structured illumination microscopy, revealing paraspeckles with dynamic, twisted elongated structures. Using siRNA knockdowns, we show these labeled paraspeckles consist of GFP-NONO/endogenous SFPQ dimers and that GFP-NONO localization to paraspeckles depends on endogenous SFPQ. Using purified proteins, we ...
Tópico(s): RNA and protein synthesis mechanisms
2022 - Elsevier BV | Journal of Biological Chemistry
... the assembly of dynamic nuclear structures (Figure 1E). Paraspeckles are discrete ribonucleoprotein bodies found in mammalian cell ... hyperedited mRNAs. Mao et al. expressed fluorescently tagged paraspeckle-associated fusion proteins in cells with an inducible Men ɛ/β lncRNA, the RNA component of paraspeckles (Mao et al., 2011Mao Y. Sunwoo H. Zhang ... nascent Men ɛ/β transcripts. They showed that paraspeckle-associated proteins were rapidly recruited and assembled on ... been shown for endogenous Men ɛ/β-containing paraspeckles. Also, like endogenous paraspeckles, the induced structures effectively ...
Tópico(s): RNA modifications and cancer
2011 - Cell Press | Cell
Yoshinori Nishimoto, Shinichi Nakagawa, Tetsuro Hirose, Hirotaka James Okano, Masaki Takao, Shinsuke Shibata, Satoshi Suyama, Ken-ichiro Kuwako, Takao Imai, Shigeo Murayama, Norihiro Suzuki, Hideyuki Okano,
... 2 (NEAT1_2), constitutes nuclear bodies known as "paraspeckles". Mutations of RNA binding proteins, including TAR DNA- ... this study was to clarify the interaction of paraspeckles with ALS-associated RNA-binding proteins, and to identify increased occurrence of paraspeckles in the nucleus of ALS spinal motor neurons. ... associated RNA-binding proteins, and to test if paraspeckles form in ALS spinal motor neurons. As the ... TDP-43 and FUS/TLS were enriched in paraspeckles and bound to NEAT1_2 lncRNA directly. The ...
Tópico(s): Neurogenetic and Muscular Disorders Research
2013 - BioMed Central | Molecular Brain
Tomohiro Yamazaki, Tetsuro Hirose,
... composed of distinct subnuclear structures termed nuclear bodies. Paraspeckles are subnuclear structures that form around the long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) together with numerous RNA- ... domain and are related to specific neurodegenerative diseases. Paraspeckle formation proceeds in conjunction with NEAT1 lncRNA biogenesis and involves the cooperation of multiple paraspeckle-localized RNA-binding proteins. NEAT1 lncRNA likely sequesters these RNA-binding proteins in paraspeckle structures, which act as molecular sponges at which ...
Tópico(s): Cancer-related molecular mechanisms research
2015 - Frontiers Media | Frontiers in Bioscience-Elite
Manon Torres, Denis Becquet, Marie‐Pierre Blanchard, Séverine Guillen, Bénédicte Boyer, Mathias Moreno, Jean‐Louis Franc, Anne‐Marie François‐Bellan,
... post-transcriptional mechanism, which involves nuclear bodies called paraspeckles. Major components of paraspeckles including the long noncoding RNA Neat1, which is ... protein partners, as well as the number of paraspeckles, follow a circadian pattern in pituitary cells. Paraspeckles are known to retain within the nucleus RNAs ... circadian expression. Moreover, circadian endogenous mRNA associated with paraspeckles lost their circadian pattern when paraspeckles were disrupted. This work not only highlights a ...
Tópico(s): Spaceflight effects on biology
2017 - Taylor & Francis | Nucleus
Yizhu Lin, Brigitte F. Schmidt, Marcel P. Bruchez, C. Joel McManus,
Paraspeckles are nuclear bodies that regulate multiple aspects of gene expression. The long non-coding RNA (lncRNA) NEAT1 is essential for paraspeckle formation. NEAT1 has a highly ordered spatial organization within the paraspeckle, such that its 5' and 3' ends localize on the periphery of paraspeckle, while central sequences of NEAT1 are found within the paraspeckle core. As such, the structure of NEAT1 RNA may be important as a scaffold for the paraspeckle. In this study, we used SHAPE probing and ...
Tópico(s): Plant and Fungal Interactions Research
2018 - Oxford University Press | Nucleic Acids Research
Haiyan An, Jing Tong Tan, Tatyana A. Shelkovnikova,
... the crosstalk between spatially separated granules remains unaddressed. Paraspeckles and stress granules (SGs) are prototypical RNP granules ... sclerosis. We characterized the composition of affinity-purified paraspeckle-like structures and found a significant overlap between the proteomes of paraspeckles and SGs. We further show that paraspeckle hyperassembly is typical for cells subjected to SG- ... is required to trigger and maintain stress-induced paraspeckle assembly. Mechanistically, SGs may sequester negative regulators of ...
Tópico(s): RNA modifications and cancer
2019 - Rockefeller University Press | The Journal of Cell Biology
Ana Bajc Česnik, Simona Darovic, Sonja Prpar Mihevc, Maja Štalekar, Mirjana Malnar, Helena Motaln, Youn‐Bok Lee, Julija Mazej, Jure Pohleven, Markus Grosch, Miha Modic, Marko Fonovič, Boris Turk, Micha Drukker, Christopher E. Shaw, Boris Rogelj,
... G4C2)n repeat RNA predominantly associates with essential paraspeckle proteins SFPQ, NONO, RBM14, FUS and hnRNPH and co-localizes with known paraspeckle-associated RNA hLinc-p21. As formation of paraspeckles in motor neurons has been associated with early ... ALS, we investigated the extent of similarity between paraspeckles and (G4C2)n RNA foci. Overexpression of (G4C2) ... co-localization with SFPQ-stained nuclear bodies. These paraspeckle-like (G4C2)72 RNA foci form independently of the known paraspeckle scaffold, the long non-coding RNA NEAT1. Moreover, ...
Tópico(s): Neurogenetic and Muscular Disorders Research
2019 - The Company of Biologists | Journal of Cell Science
Andrew T. Major, Cathryn A. Hogarth, J. Christopher Young, Yasuyuki Kurihara, David A. Jans, Kate L. Loveland,
... are markers for the distinct subnuclear domain termed paraspeckles, while a long NEAT1 isoform scaffold facilitates paraspeckle nucleation. Paraspeckles contain many proteins (>40) and are broadly involved ... Immunohistochemistry reveals cell-specific profiles for core DBHS paraspeckle protein expression, indicating their functional diversity. PSPC1 is ... lncRNA appears in the punctate nuclear foci delineating paraspeckles only within Leydig cells. Identification of NEAT1 in ... cytoplasm of spermatogonia and spermatocytes must reflect non-paraspeckle-related functions. NONO was absent from germ cells ...
Tópico(s): Molecular Biology Techniques and Applications
2019 - Bioscientifica | Reproduction
Tetsuya Yamamoto, Tomohiro Yamazaki, Tetsuro Hirose,
Paraspeckles are nuclear bodies scaffolded by RNP complexes of NEAT1_2 RNA transcripts and multiple RNA-binding proteins. The assembly of paraspeckles is coupled with the transcription of NEAT1_2. Paraspeckles form the core-shell structure, where the two ... 2 RNP complexes compose the shell of the paraspeckle and the middle regions of these complexes compose ... core. We here construct a theoretical model of paraspeckles by taking into account the transcription of NEAT1_ ... predicts that the core-shell structure of a paraspeckle is assembled by the association of the middle ...
Tópico(s): Protein Structure and Dynamics
2022 - Frontiers Media | Frontiers in Molecular Biosciences
Archa H. Fox, Charles S. Bond, Angus I. Lamond,
... presence at different nuclear locations. Here we characterize paraspeckles, a subnuclear domain containing p54nrb and other RNA- ... to p54nrb that acts as a marker for paraspeckles. We show that PSP1 interacts in vivo with ... required for the correct localization of PSP1 to paraspeckles. This interaction is necessary but not sufficient for paraspeckle targeting by PSP1, which also requires an RRM ... at the end of mitosis with DRB prevents paraspeckle formation, which recommences after removal of DRB, indicating ...
Tópico(s): Genomics and Chromatin Dynamics
2005 - American Society for Cell Biology | Molecular Biology of the Cell