Produção Nacional
Revisado por pares
Julia Schwartzman, François Lebreton, Rauf Salamzade, Terrance Shea, Melissa J. Martin, Katharina Schaufler, Aysun Urhan, Thomas Abeel, Ilana Lopes Baratella da Cunha Camargo, Bruna F. Sgardioli, Janira Prichula, Ana Paula Guedes Frazzon, Gonzalo Giribet, Daria Van Tyne, Gregg Treinish, Charles J. Innis, Jaap A. Wagenaar, Ryan M. Whipple, Abigail L. Manson, Ashlee M. Earl, Michael S. Gilmore,
Enterococci are gut microbes of most land animals. Likely appearing first in the guts of arthropods as they moved onto land, they diversified over hundreds of millions of years adapting to evolving hosts and host diets. Over 60 enterococcal species are now known. Two species,
Tópico(s): Bacterial Identification and Susceptibility Testing
2024 - National Academy of Sciences | Proceedings of the National Academy of Sciences
João Brinco, Paula Guedes, Marco Gomes da Silva, Eduardo P. Mateus, Alexandra B. Ribeiro,
This work reviews recently developed methodologies for multiclass pesticide residue analysis in soil and evaluates them under the focus of Green Analytical Chemistry principles, cost and time. Different extraction, clean-up and determination techniques are highlighted. QuEChERS was found to be the dominant form of extraction reported, although extractions using pressurized fluid, ultrasound and simple solid–liquid partitioning are still widely employed. GC–MS and LC-MS remain the standard analytical ...
Tópico(s): Pharmaceutical and Antibiotic Environmental Impacts
2023 - Elsevier BV | Microchemical Journal
Cynthia Malhadas, Ricardo Malheiro, José Alberto Pereira, Paula Guedes de Pinho, Paula Baptista,
In this study, the antimicrobial potential of three fungal endophytes from leaves of Olea europaea L. was evaluated and the host plant extract effect in the antimicrobial activity was examined. The volatile compounds produced by endophytes were identified by GC/MS and further correlated with the antimicrobial activity. In potato dextrose agar, both Penicillium commune and Penicillium canescens were the most effective inhibiting Gram-positive and -negative bacteria (up to 2.7-fold compared to 30 µg/ ...
Tópico(s): Microbial Natural Products and Biosynthesis
2017 - Springer Science+Business Media | World Journal of Microbiology and Biotechnology
Juliana Garcia, Vera Marisa Costa, Ana Elisa Costa, Sérgio Faloni de Andrade, Ana Cristina Carneiro, Filipe Conceiç�ão, José Artur Paiva, Paula Guedes de Pinho, Paula Baptista, Maria de Lourdes Bastos, Félix Carvalho,
Mushroom poisonings occur when ingestion of wild mushrooms containing toxins takes place, placing the consumers at life-threatening risk. In the present case report, an unusual multiple poisoning with isoxazoles- and amatoxins-containing mushrooms in a context of altered mental state and poorly controlled hypertension is presented. A 68-year-old female was presented to São João hospital (Portugal) with complaints of extreme dizziness, hallucinations, vertigo and imbalance, 3 h after consuming a stew ...
Tópico(s): Herbal Medicine Research Studies
2015 - Elsevier BV | Toxicon
Luís R. Silva, Maria J. Pereira, Jessica Azevedo, Rui F. Gonçalves, Patrı́cia Valentão, Paula Guedes de Pinho, Paula B. Andrade,
The consumption of sprouts, common in Asia, has been growing in western countries, once they are a natural healthy food and considered as a valuable dietary supplement. Comparing with their mature counterparts, sprouts are usually richer in health-promoting phytochemicals. So, the nutritional composition and the biological potential of widely consumed sprouts of three species – Glycine max (L.) Merr., Vigna radiata L. and Medicago sativa L. – were compared for the first time. Phenolic compounds and phytosterols ...
Tópico(s): Moringa oleifera research and applications
2012 - Elsevier BV | Food Research International
Patrı́cia Valentão, Rui F. Gonçalves, Cristóvão Belo, Paula Guedes de Pinho, Paula B. Andrade, Federico Ferreres,
Abstract Piper betle is a species growing in South East Asia, where its leaves are economically and medicinally important. To screen the highest possible number of volatile and semivolatile components, the leaves were subjected to headspace solid‐phase microextraction, hydrodistillation and Soxhlet extraction, prior to analysis by GC/MS. Fifty compounds (identified by comparison with standard compounds or tentatively by National Institute of Standards and Technology database) were determined, 23 ...
Tópico(s): Plant-based Medicinal Research
2010 - Wiley | Journal of Separation Science
Marcos Taveira, Luís R. Silva, Luís A. Vale-Silva, Eugénia Pinto, Patrı́cia Valentão, Federico Ferreres, Paula Guedes de Pinho, Paula B. Andrade,
Lycopersicon esculentum (tomato) fruit is a widely studied matrix. However, only few works focus their attention on its seeds, which constitute a major byproduct of the tomato processing industry. In this study the antimicrobial potential of ten different tomato seed extracts from "Bull's heart" and "Cherry" varieties were analyzed against Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Enterococcus faecalis and Bacillus cereus) and Gram-negative (Proteus mirabilis, ...
Tópico(s): Phytochemicals and Antioxidant Activities
2010 - American Chemical Society | Journal of Agricultural and Food Chemistry
Marcos Taveira, Fátima Fernandes, Paula Guedes de Pinho, Paula B. Andrade, José Alberto Pereira, Patrı́cia Valentão,
Brassica rapa var. rapa L. (turnip) is highly appreciated and consumed by human. In this work, the volatile profile of B. rapa var. rapa was studied during the maturation process. The volatiles of seeds, sprouts with 6 and 9 days, and adult plant were determined using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap-mass spectrometry (GC/IT-MS). Several constituents, including alcohols, aldehydes, esters, ketones, norisoprenoids, nitrogen and sulphur compounds ...
Tópico(s): Nitrogen and Sulfur Effects on Brassica
2009 - Elsevier BV | Microchemical Journal
Bárbara Ribeiro, Paula Guedes de Pinho, Paula B. Andrade, Paula Baptista, Patrı́cia Valentão,
A comparative study was developed on the total fatty acids composition of twelve wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, Cantharellus cibarius, Amanita caesarea and Hydnum rufescens). In order to define qualitative and quantitative profiles, combined fatty acids were hydrolyzed with potassium hydroxide/methanol and all free compounds were ...
Tópico(s): Polysaccharides and Plant Cell Walls
2009 - Elsevier BV | Microchemical Journal
A. C. Silva Ferreira, Paula Guedes de Pinho, Paula Rodrigues, Tim Hogg,
The negative effects of oxygen on white wine quality and the various factors which influence it (including temperature, dissolved oxygen, pH, and free SO(2)) are well documented both at the sensory and compositional levels. What is less defined is the quantitative relationship between these parameters and the kinetics of the development of the negative effects of oxidation. The experiment presented here attempts to generate data which can be used to predictively model the oxidative degradation of ...
Tópico(s): Phytochemicals and Antioxidant Activities
2002 - American Chemical Society | Journal of Agricultural and Food Chemistry
Javier Pérez-García, Mario A. González Carracedo, Antonio Espuela‐Ortiz, José María Hernández Pérez, Ruperto González‐Pérez, Olaia Sardón, Elena Martín-González, Elena Mederos‐Luis, Paloma Poza‐Guedes, Paula Corcuera, Ariel Callero, Inmaculada Sánchez‐Machín, Javier Korta‐Murua, José Antonio Pérez Pérez, Jesús Villar, María Pino-Yanes, Fabián Lorenzo-Díaz,
BackgroundThe response to inhaled corticosteroids (ICS) in asthma is affected by the interplay of several factors. Among these, the role of the upper-airway microbiome has been scarcely investigated. We aimed to evaluate the association between the salivary, pharyngeal, and nasal microbiome with asthma exacerbations despite receipt of ICS.MethodsSamples from 250 asthma patients from the Genomics and Metagenomics of Asthma Severity (GEMAS) study treated with ICS were analyzed. Control/case subjects ...
Tópico(s): Respiratory and Cough-Related Research
2022 - Elsevier BV | Journal of Allergy and Clinical Immunology
Ana Catarina Guedes, Paula Ludovico, Belém Sampaio‐Marques,
Alpha-synuclein (syn) is the main component of proteinaceous inclusions known as Lewy bodies (LBs), which are implicated in the pathogenesis of the neurodegenerative diseases known as synucleinopathies, like Parkinson's disease (PD). Aging is a major risk factor for PD and thus, interventions that delay aging will have promising effects in PD and other synucleinopathies. Caloric restriction (CR) is the only non-genetic intervention shown to promote lifespan extension in several model organisms. CR has ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2016 - Elsevier BV | Mechanisms of Ageing and Development
David Guede, Paula Álvarez‐González, José Ramón Caeiro,
The principals of classical mechanics are applied to the study of the resistance of materials to fracture when subject to a certain load.Bone has been, for a long time, the object of study in the field of mechanics in order to understand and resolve problems of fracture associated with deficient mechanical behaviour which may exist due to factors such as age or certain pathologies.The great quantity of specific vocabulary used in biomechanics, derived as it is from the terminology of mechanical ...
Tópico(s): Bone health and osteoporosis research
2013 - SciELO | Revista de Osteoporosis y Metabolismo Mineral
Isabel P. Martins, Tania Guedes, Paula Rama, João Carlos Ramos, Tatiana Tchemisova,
A food bank is a non-profit, social solidarity organisation that typically distributes the donated food among a wide variety of local non-profit, social solidarity institutions which in turn feed the low-income people. The problem presented by the Portuguese Federation of Food Banks is to determine, for a specific food bank, the quantities of the donated products that must be assigned to each local social solidarity institution in order to satisfy the needs of the supported people as much as possible, ...
Tópico(s): Optimization and Mathematical Programming
2011 - Inderscience Publishers | International Journal of Mathematical Modelling and Numerical Optimisation
Ivo Oliveira, Paula Guedes de Pinho, Ricardo Malheiro, Paula Baptista, José Alberto Pereira,
Arbutus unedo L. is a Mediterranean shrub used in the production of jams, marmalades and beverages, as well as in traditional medicine. In this work, the effect of ripening on the volatile compounds of the fruits of A. unedo was evaluated, using headspace-solid phase microextraction combined with gas chromatography/ion trap-mass spectrometry. Alcohols are the main component of the volatile fraction of A. unedo fruits, followed by aldehydes and esters. The main volatile compounds identified were ( ...
Tópico(s): Phytochemicals and Antioxidant Activities
2011 - Elsevier BV | Food Chemistry
Branca M. Silva, Rui Santos, Lídia S. Mendes, Paula Guedes de Pinho, Patrı́cia Valentão, Paula B. Andrade, José Alberto Pereira, Márcia Carvalho,
The present study reports for the first time the metabolite profile and antioxidant activity of aqueous extract obtained from Dracaena draco L. fruit. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 9 compounds being identified, distributed by several distinct chemical classes: 1 alcohol, 3 aldehydes, 2 carotenoid derivatives, and 3 terpenic compounds. Aldehydes constituted the most abundant class in this exotic berry, representing 59% of total identified volatile compounds. Phenolics ...
Tópico(s): Natural product bioactivities and synthesis
2010 - Elsevier BV | Food Research International
Patrı́cia Valentão, Pedro Trindade, Daniela Gomes, Paula Guedes de Pinho, Teresa Mouga, Paula B. Andrade,
The chemical composition of green Codium tomentosum Stackhouse and red Plocamium cartilagineum (Linnaeus) P.S. Dixon seaweeds from the Atlantic Ocean surrounding Portugal was studied. For the first time, the profile of organic acids was analysed in these matrices: seven and four organic acids were detected in green and red species, respectively. In P. cartilagineum these compounds were present in vestigial amounts, whilst C. tomentosum exhibited a higher content, with oxalic acid being the main compound. ...
Tópico(s): Collagen: Extraction and Characterization
2009 - Elsevier BV | Food Chemistry
Marcos Taveira, Paula Guedes de Pinho, Rui F. Gonçalves, Paula B. Andrade, Patrı́cia Valentão,
Tópico(s): Plant biochemistry and biosynthesis
2009 - Elsevier BV | Microchemical Journal
Produção Nacional
Revisado por pares
Mara Heckmann, Nadiia Sadova, Ivana Drotarova, Stefanie Atzmüller, Bettina Schwarzinger, Roberto Maurício Carvalho Guedes, Paula A. Correia, Stefan Hirtenlehner, Christine Potthast, Gerald Klanert, Julian Weghuber,
Lignans are known to exhibit a broad spectrum of biological activities, indicating their potential as constituents of feed supplements. This study investigated two extracts derived from the feed supplements 'ROI' and 'Protect'-which contain the wood lignans magnolol and honokiol ('ROI'), or soluble tannins additional to the aforementioned lignans ('Protect')-and their impact on selected parameters of intestinal functionality. The antioxidant and anti-inflammatory properties of the extracts were determined ...
Tópico(s): Bee Products Chemical Analysis
2022 - Multidisciplinary Digital Publishing Institute | Molecules
Daniel Gomes, Paula Guedes de Pinho, Helena Pontes, Luı́sa M. Ferreira, Paula S. Branco, Fernando Remião, Félix Carvalho, Maria de Lourdes Bastos, Helena Carmo,
The investigation of 3,4-methylenedioxymethamphetamine (MDMA; ecstasy) abuse requires very robust methods with high sensitivity and wide linearity ranges for the quantification of this drug of abuse and its main metabolites in body fluids. An optimized gas chromatography–ion trap mass spectrometry (GC–IT/MS) methodology with electron impact ionization addressing these issues is presented. The sample preparation involves an enzymatic hydrolysis of urine and plasma for conjugate cleavage, a SPE extraction, ...
Tópico(s): Cannabis and Cannabinoid Research
2010 - Elsevier BV | Journal of Chromatography B
Fátima Fernandes, Paula Guedes de Pinho, Patrı́cia Valentão, José Alberto Pereira, Paula B. Andrade,
In this work, the volatile composition of kale (Brassica oleracea L. var. acephala) and its variation during germination were monitored during the first 9 days of seedling development by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap−mass spectrometry (GC/IT-MS). Differences were found among the materials in the distinct analyzed periods. A total of 66 volatile compounds, distributed in several chemical classes, were determined: alcohols, carbonyl compounds ( ...
Tópico(s): Phytochemicals and Antioxidant Activities
2009 - American Chemical Society | Journal of Agricultural and Food Chemistry
Paula Guedes de Pinho, Bárbara Ribeiro, Rui F. Gonçalves, Paula Baptista, Patrı́cia Valentão, Rosa M. Seabra, Paula B. Andrade,
Volatile and semivolatile components of 11 wild edible mushrooms, Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, and Cantharellus cibarius, were determined by headspace solid-phase microextraction (HS-SPME) and by liquid extraction combined with gas chromatography-mass spectrometry (GC-MS). Fifty volatiles and nonvolatiles components were formally ...
Tópico(s): Phytochemistry and Bioactivity Studies
2008 - American Chemical Society | Journal of Agricultural and Food Chemistry
Juliana Garcia, Ana F. Oliveira, Paula Guedes de Pinho, Víctor de Freitas, Alexandra T. P. Carvalho, Paula Baptista, Eric Pereira, Maria de Lourdes Bastos, Félix Carvalho,
Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. α-Amanitin ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2015 - Taylor & Francis | Mycologia
David M. Pereira, Juliana Vinholes, Paula Guedes de Pinho, Patrı́cia Valentão, Teresa Mouga, Natércia Teixeira, Paula B. Andrade,
In this work a fast and simple multi-target gas chromatography-mass spectrometry (GC-MS) method for the simultaneous detection and absolute quantification of amino acids, fatty acids, sterols and lupanes in marine organisms is proposed. The methodology was applied to the characterization of the echinoderm Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of target compounds were evaluated by using different extraction procedures, solvent systems ...
Tópico(s): Aquaculture Nutrition and Growth
2012 - Elsevier BV | Talanta
Esther Trigueros, Andreia P. Oliveira, Paula B. Andrade, Romeu A. Videira, Paula Guedes de Pinho, Marı́a Teresa Sanz, Sagrario Beltrán,
Gelidium sesquipedale is valued in the Spanish agar industry, but its production generates substantial waste, often discarded despite its nutritional and bioactive content. Subcritical water extraction (SWE) at 175 °C and 50 bar for 130 min was performed on this waste after agar extraction, comparing it to conventional ethanol extraction. The SWE extract exhibited superior nutritional profile, including proteins (170.6 ± 1.0 mg/g
Tópico(s): Algal biology and biofuel production
2024 - Elsevier BV | Food Chemistry
Ricardo Malheiro, Paula Guedes de Pinho, Sandra de Aguiar Soares, A. C. Silva Ferreira, Paula Baptista,
Secondary metabolites present in the volatile fraction of six wild mushroom species (Clitocybe odora, Clitocybe fragrans, Hebeloma crustuliniforme, Lepista nuda, Tricholoma fracticum and Tricholoma terreum) were studied, as an attempt to identify compounds capable to distinguish mushroom species for taxonomic and authentication purposes. Volatiles were assessed by headspace solid phase microextraction (HS–SPME) and by gas chromatography/ion-trap mass-spectrometry (GC/IT–MS). By using target analysis, ...
Tópico(s): Phytochemicals and Antioxidant Activities
2013 - Elsevier BV | Food Research International
Paula Guedes de Pinho, Patrı́cia Valentão, Rui F. Gonçalves, Carla Sousa, Paula B. Andrade,
Volatile and semi-volatile components of internal and external leaves of Brassica oleracea L. var. costata DC, grown under different fertilization regimens, were determined by headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/ion trap mass spectrometry (GC/ITMS). Forty-one volatiles and non-volatile components were formally identified and thirty others were tentatively identified. Qualitative and quantitative differences were noticed between internal and external leaves. ...
Tópico(s): Plant biochemistry and biosynthesis
2009 - Wiley | Rapid Communications in Mass Spectrometry
Fátima Fernandes, David M. Pereira, Paula Guedes de Pinho, Patrı́cia Valentão, José Alberto Pereira, Albino Bento, Paula B. Andrade,
The influence of Pieris brassicae feeding on kale was monitored, by evaluating its effect on the volatiles released by the plant through time. This is the first study applying headspace solid-phase microextraction (HS-SPME) and gas chromatography/ion trap-mass spectrometry to an isolated insect, as most studies analyse the insect-plant system as a whole, being unable to evaluate the contribution of the insect itself. Substantial differences were noticed between the volatiles composition of kale before ...
Tópico(s): Insect and Pesticide Research
2009 - Elsevier BV | Food Chemistry
Clara Grosso, Juliana Vinholes, Luís R. Silva, Paula Guedes de Pinho, Rui F. Gonçalves, Patrı́cia Valentão, Anna K. Jäger, Paula B. Andrade,
Capsella bursa-pastoris (L.) Medik. (Brassicaceae) is a wild herb with high nutritional value that can be eaten raw or cooked. A metabolomic study was performed with different extracts of its aerial parts that were tested concerning their antiradical, acetylcholinesterase inhibitory and antibacterial activities. Phenolic compounds were identified and quantified by HPLC-DAD, organic acids and amino acids were determined by HPLC-UV, while free fatty acids and sterols were analysed by GC-ITMS. The vegetal ...
Tópico(s): Essential Oils and Antimicrobial Activity
2011 - Springer Science+Business Media | Revista Brasileira de Farmacognosia
Andreia P. Oliveira, Luís R. Silva, Federico Ferreres, Paula Guedes de Pinho, Patrı́cia Valentão, Branca M. Silva, José Alberto Pereira, Paula B. Andrade,
Ficus species possess latex-like material within their vasculatures, affording protection and self-healing from physical attacks. In this work, metabolite profiling was performed on Ficus carica latex. Volatiles profile was determined by HS-SPME/GC−IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 5 aldehydes, 7 alcohols, 1 ketone, 9 monoterpenes, 9 sesquiterpenes and 3 other compounds. Sesquiterpenes constituted the most abundant class in latex (ca. 91% of total ...
Tópico(s): Hibiscus Plant Research Studies
2010 - American Chemical Society | Journal of Agricultural and Food Chemistry