Nigel Hawker, Brian Jeffery, Paulo De Almeida, Edward Gacek, Paul Clermont, Ron St. John,
... none Ukraine British Gas Diners Club International Virgin Cavaco's gamble Portugal Race for votes, votes for ...
1991 - Gale Group | The Economist
Kosuke Ito, Masayuki Nakanishi, Woo‐Cheol Lee, Yuehua Zhi, Hiroshi Sasaki, Shuhei Zenno, Kaoru Saigo, Yukio Kitade, Masaru Tanokura,
... Google Scholar, 19Maier J. Kandelbauer A. Erlacher A. Cavaco-Paulo A. Gubitz G.M. Appl. Environ. Microbiol. 2004; ... 202) Google Scholar, 20Ramalho P.A. Paiva S. Cavaco-Paulo A. Casal M. Cardoso M.H. Ramalho M. ...
Tópico(s): Photosynthetic Processes and Mechanisms
2008 - Elsevier BV | Journal of Biological Chemistry
Andreia Vasconcelos, Giuliano Freddi, Artur Cavaco‐Paulo,
... Fibroin and KeratinAndreia Vasconcelos, Giuliano Freddi, and Artur Cavaco-Paulo*Cite this: Biomacromolecules 2009, 10, 4, 1019Publication Date ( ...
Tópico(s): Dyeing and Modifying Textile Fibers
2009 - American Chemical Society | Biomacromolecules
Madalena Martins, Nuno G. Azóia, Ulyana Shimanovich, Teresa Matamá, Andreia C. Gomes, Carla Silva, Artur Cavaco‐Paulo,
A novel transdermal hyaluronic acid (HA) conjugated with bovine serum albumin (BSA) was developed in the form of solid-in-oil (S/O) nanodispersion (129.7 nm mean diameter). Ex vivo skin penetration analysis by fluorescence and confocal observation of histological skin sections revealed the ability of BSA/HA nanodispersions to cross the stratum corneum and penetrate into the dermis. Furthermore, no significant toxicity was found in fibroblast and keratinocyte cells in vitro. These results proved the ...
Tópico(s): Advanced Drug Delivery Systems
2014 - American Chemical Society | Molecular Pharmaceutics
Ya Zhang, Aixue Dong, Qiang Wang, Xuerong Fan, Artur Cavaco‐Paulo, Ying Zhang,
The high-redox-potential catalyst laccase, isolated from Aspergillus, was first used as a biocatalyst in the oxidative polymerization of water-soluble conductive polyaniline, and then conductive cotton was prepared by in situ polymerization under the same conditions. The polymerization of aniline was performed in a water dispersion of sodium dodecylbenzenesulfonate (SDBS) micellar solution with atmospheric oxygen serving as the oxidizing agent. This method is ecologically clean and permits a greater ...
Tópico(s): Electrochemical Analysis and Applications
2014 - Springer Science+Business Media | Applied Biochemistry and Biotechnology
Idalina Gonçalves, Ana S. Abreu, Teresa Matamá, Artur Ribeiro, Andreia C. Gomes, Carla Silva, Artur Cavaco‐Paulo,
Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase ...
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
2014 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Ana Loureiro, Ana S. Abreu, Marisa P. Sárria, Mafalda C. O. Figueiredo, Lı́gia M. Saraiva, Gonçalo J. L. Bernardes, Andreia C. Gomes, Artur Cavaco‐Paulo,
BSA-bioconjugates were synthesized for production of functionalized protein-based nanoemulsions using high pressure homogenization. These PEGylated nanoemulsions are designed for intravenous administration and drug delivery.
Tópico(s): Proteins in Food Systems
2014 - Royal Society of Chemistry | RSC Advances
Ulyana Shimanovich, Gonçalo J. L. Bernardes, Tuomas P. J. Knowles, Artur Cavaco‐Paulo,
Micro- and nano-scale systems have emerged as important tools for developing clinically useful drug delivery systems. In this tutorial review, we discuss the exploitation of biomacromolecules for this purpose, focusing on proteins, polypeptides, nucleic acids and polysaccharides and mixtures thereof as potential building blocks for novel drug delivery systems. We focus on the mechanisms of formation of micro- and nano-scale protein-based capsules and shells, as well as on the functionalization of ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2013 - Royal Society of Chemistry | Chemical Society Reviews
Andreia Vasconcelos, Artur Cavaco‐Paulo,
Keratins are naturally derived proteins that can be fabricated into several biomaterials morphologies including films, sponges and hydrogels. As a physical matrix, keratin biomaterials have several advantages of both natural and synthetic materials that are useful in tissue engineering and controlled released applications. Like other naturally derived protein biomaterials, such as collagen, keratin possess amino acid sequences, similar to the ones found on extracellular matrix (ECM), that may interact ...
Tópico(s): Skin Protection and Aging
2013 - Bentham Science Publishers | Current Drug Targets
Produção Nacional
Revisado por pares
Daniela Gomes, Teresa Matamá, Artur Cavaco‐Paulo, Galba Maria de Campos‐Takaki, Alexandra A. Salgueiro,
Background:The hydrolytic action of cutinases has been applied to the degradation of plastics.Polyethylene terephthalate (PET) have long half-life which constitutes a major problem for their treatment as urban solid residues.The aim of this work was to characterize and to improve stable the enzyme to optimize the process of degradation using enzymatic hydrolysis of PET by recombinant cutinases. Results:The wild type form of cutinase from Fusarium solani pisi and its C-terminal fusion to cellulose ...
Tópico(s): Animal Genetics and Reproduction
2013 - Elsevier BV | Electronic Journal of Biotechnology
Carla Silva, Teresa Matamá, Nuno G. Azóia, Catarina Mansilha, Margarida Casal, Artur Cavaco‐Paulo,
The application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli, and the purified protein was biochemically characterized. The IC50 values (concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fragrances, namely, citronellol, benzyl benzoate, citronellyl valerate and ethyl valerate. ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2013 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Helena Ferreira, Teresa Matamá, Raquel Silva, Carla Silva, Andreia C. Gomes, Artur Cavaco‐Paulo,
From ancientness, suitable materials have been developed to cover the wounds in order to prevent infections and promote proper wound healing. In this study, the successful development of functionalized nonwoven gauzes with liposomes entrapping anti-inflammatory piroxicam is reported. Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) that can suppress a persistent inflammatory response, leading to improved wound healing. The results demonstrated that the highest NSAID concentration released ...
Tópico(s): Surgical Sutures and Adhesives
2013 - Elsevier BV | Reactive and Functional Polymers
Agnes Stépán, George E Anasontzis, Teresa Matamá, Artur Cavaco‐Paulo, Lisbeth Olsson, Paul Gatenholm,
This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX films and that a cutinase from Fusarium solani pisi surface acetylates these films. The ...
Tópico(s): Enzyme Catalysis and Immobilization
2013 - Elsevier BV | Journal of Biotechnology
Célia F. Cruz, Margarida M. Fernandes, Andreia C. Gomes, L. Coderch, Meritxell Martı́, S. Méndez, Luı́s Gales, Nuno G. Azóia, Ulyana Shimanovich, Artur Cavaco‐Paulo,
Synopsis Human hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also report the study of the interaction between those two main components of hair, ...
Tópico(s): Skin and Cellular Biology Research
2013 - Wiley | International Journal of Cosmetic Science
Idalina Gonçalves, Madalena Martins, Ana Loureiro, Andreia C. Gomes, Artur Cavaco‐Paulo, Carla Silva,
The main goal of this work is to develop a novel and environmental-friendly technology for cotton bleaching with reduced processing costs. This work exploits a combined laccase-hydrogen peroxide process assisted by ultrasound. For this purpose, specific reactors were studied, namely ultrasonic power generator type K8 (850 kHz) and ultrasonic bath equipment Ultrasonic cleaner USC600TH (45 kHz). The optimal operating conditions for bleaching were chosen considering the highest levels of hydroxyl radical ...
Tópico(s): Dermatologic Treatments and Research
2013 - Elsevier BV | Ultrasonics Sonochemistry
Artur Ribeiro, Teresa Matamá, Célia F. Cruz, Andreia C. Gomes, Artur Cavaco‐Paulo,
The objective of this work was to develop a new strategy to physically 'repair' chemically damaged hair. Hence the human eye γD-crystallin, a protein from the superfamily characterized structurally by the Greek key motif, was studied. The human γD-crystallin was chosen based on the ability of proteins belonging to this superfamily to be involved in the coating of specific structures. Two crystallins were used on the study, the wild type (Protein Data Bank ID: 1HK0) and the mutant protein. The mutant ...
Tópico(s): melanin and skin pigmentation
2013 - Wiley | International Journal of Cosmetic Science
Madalena Martins, Nuno G. Azóia, Artur Ribeiro, Ulyana Shimanovich, Carla Silva, Artur Cavaco‐Paulo,
Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.
Tópico(s): Proteins in Food Systems
2013 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Eugénia Nogueira, Ana Loureiro, Patrícia Nogueira, Jaime Freitas, Catarina R. Almeida, Johan Härmark, Hans Hebert, Alexandra Moreira, Alexandre M. Carmo, Ana Preto, Andreia C. Gomes, Artur Cavaco‐Paulo,
Liposomes and protein based nanoparticles were tuned with different polymers and glycolipids to improve stealth and thus decrease their clearance by macrophages. Liposomes were coated with polyethylene glycol (PEG) and brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) nanoparticles were produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained nanoparticles were monodisperse, with sizes ranging from 80 to 120 nm, with a zeta-potential close to zero. The presented ...
Tópico(s): Characterization and Applications of Magnetic Nanoparticles
2013 - Royal Society of Chemistry | Faraday Discussions
Alexandra Rollett, Tamara Reiter, Patrícia Nogueira, Massimiliano Cardinale, Ana Loureiro, Andreia C. Gomes, Artur Cavaco‐Paulo, Alexandra Moreira, Alexandre M. Carmo, Georg M. Guebitz,
Activated synovial macrophages play a key role in Rheumatoid Arthritis (RA). Recent studies have shown that folate receptor beta (FRβ) is specifically expressed by activated macrophages. Therefore a folate-based nanodevice would provide the possibility of delivering therapeutic agents to activated macrophages without affecting normal cells and tissues. This study shows for the first time the sonochemical preparation of HSA nanocapsules avoiding toxic cross linking chemicals and emulsifiers used in ...
Tópico(s): Advancements in Transdermal Drug Delivery
2012 - Elsevier BV | International Journal of Pharmaceutics
Margarida M. Fernandes, Cristóvão F. Lima, Ana Loureiro, Andreia C. Gomes, Artur Cavaco‐Paulo,
A peptide based on a fragment of hair keratin type II cuticular protein, keratin peptide (KP), was studied as a possible strengthening agent for weakened relaxed hair. The peptide was prepared both in aqueous water formulation (WF) and organic solvent formulations (OF), to determine the effect of organic solvents on peptide interaction with hair and the differences in hair recovery. Both peptide formulations were shown to improve mechanical and thermal properties of weakened hair with peptide in OF ...
Tópico(s): Bee Products Chemical Analysis
2012 - Wiley | International Journal of Cosmetic Science
Ulyana Shimanovich, Dror Eliaz, Sally Zigdon, Vadim Volkov, Adva Aizer, Artur Cavaco‐Paulo, Shulamit Michaeli, Yaron Shav‐Tal, Aharon Gedanken,
In the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the "ultrasonic emulsification method". It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA-RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. ...
Tópico(s): Virus-based gene therapy research
2012 - Royal Society of Chemistry | Journal of Materials Chemistry B
Andreia Vasconcelos, Andreia C. Gomes, Artur Cavaco‐Paulo,
Silk fibroin (SF) and elastin (EL) scaffolds were successfully produced for the first time for the treatment of burn wounds. The self-assembly properties of SF, together with the excellent chemical and mechanical stability and biocompatibility, were combined with elastin protein to produce scaffolds with the ability to mimic the extracellular matrix (ECM). Porous scaffolds were obtained by lyophilization and were further crosslinked with genipin (GE). Genipin crosslinking induces the conformational ...
Tópico(s): Bone Tissue Engineering Materials
2012 - Elsevier BV | Acta Biomaterialia
Jiajia Fu, Gibson S. Nyanhongo, Georg Gübitz, Artur Cavaco‐Paulo, Suyeon Kim,
Enzymes have received significant attention as alternative catalysts to chemical auxiliaries in textile processing. For example, laccases and peroxidises are promising alternatives for bleaching and denim stone washing processes. Similarly, the ability to oxidise different phenolic substrates and dye precusors resulting in the formation of different coloured polymeric molecules is being exploited for developing green chemistry dyeing processes. The enzymatic process is simpler than conventional ...
Tópico(s): Dyeing and Modifying Textile Fibers
2012 - Taylor & Francis | Biocatalysis and Biotransformation
Jiajia Fu, Xingqun Zhang, Chongwen Yu, Georg M. Guebitz, Artur Cavaco‐Paulo,
Bamboo culms were processed using microorganisms, and fibre bundles were obtained. Bacteria and fungi with xylanase activity were isolated from the bamboo retting system. Chemical composition analysis of the fibre bundles obtained showed that the components are mainly cellulose, hemicelluloses and lignin. An increase in cellulose and hemicelluloses content was detected along with a decrease in lignin content after bio-processing. Environmental Scanning Electronic Microcopy of the fibre bundles (retted) ...
Tópico(s): Advanced Cellulose Research Studies
2012 - Index Copernicus International S.A. | Fibres and Textiles in Eastern Europe
Suyeon Kim, Margarida M. Fernandes, Teresa Matamá, Ana Loureiro, Andreia C. Gomes, Artur Cavaco‐Paulo,
Due to their recognised properties of biocompatibility, biodegradability and sustainability, chitosan nanocarriers have been successfully used as new delivery systems. In this work, nanoparticles combining chitosan and lignosulfonates were developed for the first time for cosmetic and biomedical applications. The ability of lignosulfonates to act as a counter polyion for stabilisation of chitosan particles, generated using high intensity ultrasound, was investigated. Several conditions for particles ...
Tópico(s): Nanomaterials and Printing Technologies
2012 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Jiajia Fu, Xiaoqiang Li, Weidong Gao, Hongbo Wang, Artur Cavaco‐Paulo, Carla Silva,
This review addresses the potential biotechnological techniques for textile fibre extraction (degumming), mainly focusing on bamboo, one of the main and recently developed textile natural fibres in China. The latest developments in microbial and enzymatic processing for ecological fibre extraction as well as the development of a molecular technique for natural fibre bio-treatment are highlighted.
Tópico(s): Biofuel production and bioconversion
2012 - Taylor & Francis | Biocatalysis and Biotransformation
Cristina Caparrós, Neil J. Lant, Johan Smets, Artur Cavaco‐Paulo,
The impacts of two hybrid cloned commercial cellulases designed for detergency on cotton fibres were compared. HiCel45 has a family 45 catalytic domain and a fungal cellulose binding module (CBM) from the fungus Humicola insolens. BaCel5 has a family 5 catalytic domain and a fungal CBM from Bacillus spp. BaCel5 bound irreversibly to cellulose under the buffer conditions tested while HiCel45 was found to bind reversibly to cellulose because it showed low adsorption. BaCel5 seems to yield more activity ...
Tópico(s): Enzyme Production and Characterization
2012 - Taylor & Francis | Biocatalysis and Biotransformation
Oshrat Tzhayik, Artur Cavaco‐Paulo, Aharon Gedanken,
Protein microspheres have been prepared by sonicating a mixture of pure fragrant oil (amyl acetate (AA)) with an aqueous protein (bovine serum albumin) solution. The prepared protein spheres are nano- to micrometer sized with an encapsulation efficiency of approx. 97% for the AA present on the surface and inside the BSA capsule. Containers were found stable for more than 6 months when stored sealed at 4 °C and 20 °C. For the release profile measurements, we used a simple, automated and direct method. ...
Tópico(s): Innovative Microfluidic and Catalytic Techniques Innovation
2012 - Elsevier BV | Ultrasonics Sonochemistry
Raquel Silva, Helena Ferreira, Nuno G. Azóia, Ulyana Shimanovich, Giuliano Freddi, Aharon Gedanken, Artur Cavaco‐Paulo,
Microspheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the interface. Fourier transform infrared results indicate a large increase in the content ...
Tópico(s): Proteins in Food Systems
2012 - American Chemical Society | Molecular Pharmaceutics
Alexandra Rollett, Barbara Thallinger, Anna Ohradanova‐Repic, Christian Machacek, Evelyn Walenta, Artur Cavaco‐Paulo, Ruth Birner‐Gruenberger, Juliane Gertrude Bogner‐Strauß, Hannes Stockinger, Georg M. Guebitz,
Highly specific targeted drug delivery devices can be obtained with antibody-human serum albumin (mAb-HSA) conjugates. However, their conventional production involves several reaction steps including chemical modification and activation of both proteins followed by cross-linking often involving toxic chemicals. Here, we describe the enzymatic synthesis of mAb-HSA conjugates for targeted drug delivery devices using tyrosinase from Agaricus bisporus under mild reaction conditions (pH 6.8, 25 °C). Reaction ...
Tópico(s): Glycosylation and Glycoproteins Research
2012 - Royal Society of Chemistry | RSC Advances