Nigel Hawker, Brian Jeffery, Paulo De Almeida, Edward Gacek, Paul Clermont, Ron St. John,
... none Ukraine British Gas Diners Club International Virgin Cavaco's gamble Portugal Race for votes, votes for ...
1991 - Gale Group | The Economist
Kosuke Ito, Masayuki Nakanishi, Woo‐Cheol Lee, Yuehua Zhi, Hiroshi Sasaki, Shuhei Zenno, Kaoru Saigo, Yukio Kitade, Masaru Tanokura,
... Google Scholar, 19Maier J. Kandelbauer A. Erlacher A. Cavaco-Paulo A. Gubitz G.M. Appl. Environ. Microbiol. 2004; ... 202) Google Scholar, 20Ramalho P.A. Paiva S. Cavaco-Paulo A. Casal M. Cardoso M.H. Ramalho M. ...
Tópico(s): Photosynthetic Processes and Mechanisms
2008 - Elsevier BV | Journal of Biological Chemistry
Andreia Vasconcelos, Giuliano Freddi, Artur Cavaco‐Paulo,
... Fibroin and KeratinAndreia Vasconcelos, Giuliano Freddi, and Artur Cavaco-Paulo*Cite this: Biomacromolecules 2009, 10, 4, 1019Publication Date ( ...
Tópico(s): Dyeing and Modifying Textile Fibers
2009 - American Chemical Society | Biomacromolecules
Ya Zhang, Aixue Dong, Qiang Wang, Xuerong Fan, Artur Cavaco‐Paulo, Ying Zhang,
The high-redox-potential catalyst laccase, isolated from Aspergillus, was first used as a biocatalyst in the oxidative polymerization of water-soluble conductive polyaniline, and then conductive cotton was prepared by in situ polymerization under the same conditions. The polymerization of aniline was performed in a water dispersion of sodium dodecylbenzenesulfonate (SDBS) micellar solution with atmospheric oxygen serving as the oxidizing agent. This method is ecologically clean and permits a greater ...
Tópico(s): Electrochemical Analysis and Applications
2014 - Springer Science+Business Media | Applied Biochemistry and Biotechnology
Ana Loureiro, Ana S. Abreu, Marisa P. Sárria, Mafalda C. O. Figueiredo, Lı́gia M. Saraiva, Gonçalo J. L. Bernardes, Andreia C. Gomes, Artur Cavaco‐Paulo,
BSA-bioconjugates were synthesized for production of functionalized protein-based nanoemulsions using high pressure homogenization. These PEGylated nanoemulsions are designed for intravenous administration and drug delivery.
Tópico(s): Proteins in Food Systems
2014 - Royal Society of Chemistry | RSC Advances
Idalina Gonçalves, Ana S. Abreu, Teresa Matamá, Artur Ribeiro, Andreia C. Gomes, Carla Silva, Artur Cavaco‐Paulo,
Biofilm formation in urinary indwelling catheters is one of the most critical issues that patients face. Catheters were coated with poly(catechin)-antibiotic conjugates with enhanced antimicrobial properties. Catechin was conjugated with two antibiotics, namely trimethoprim (TMP) and sulfamethoxazole (SMZ) via activation with N,N′-disuccinimidyl carbonate (DSC) and subsequent coupling to molecules containing α-amine moieties. Silicone and polyurethane catheters were functionalized in situ through laccase ...
Tópico(s): Phenothiazines and Benzothiazines Synthesis and Activities
2014 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Idalina Gonçalves, Victor Herrero-Yniesta, Iratxe Perales Arce, Monica Escrigas Castañeda, Artur Cavaco‐Paulo, Carla Silva,
The potential of ultrasound-assisted technology has been demonstrated by several laboratory scale studies. However, their successful industrial scaling-up is still a challenge due to the limited pilot and commercial sonochemical reactors. In this work, a pilot reactor for laccase-hydrogen peroxide cotton bleaching assisted by ultrasound was scaled-up. For this purpose, an existing dyeing machine was transformed and adapted by including piezoelectric ultrasonic devices. Laboratory experiments demonstrated ...
Tópico(s): Biocrusts and Microbial Ecology
2014 - Elsevier BV | Ultrasonics Sonochemistry
Idalina Gonçalves, Carla Silva, Artur Cavaco‐Paulo,
Combined laccase–ultrasound systems allow the reduction of both the environmental impact and the operational costs of conventional industrial processing.
Tópico(s): Electrochemical sensors and biosensors
2014 - Royal Society of Chemistry | Green Chemistry
Madalena Martins, Nuno G. Azóia, Ulyana Shimanovich, Teresa Matamá, Andreia C. Gomes, Carla Silva, Artur Cavaco‐Paulo,
A novel transdermal hyaluronic acid (HA) conjugated with bovine serum albumin (BSA) was developed in the form of solid-in-oil (S/O) nanodispersion (129.7 nm mean diameter). Ex vivo skin penetration analysis by fluorescence and confocal observation of histological skin sections revealed the ability of BSA/HA nanodispersions to cross the stratum corneum and penetrate into the dermis. Furthermore, no significant toxicity was found in fibroblast and keratinocyte cells in vitro. These results proved the ...
Tópico(s): Advanced Drug Delivery Systems
2014 - American Chemical Society | Molecular Pharmaceutics
Célia F. Cruz, Margarida M. Fernandes, Andreia C. Gomes, L. Coderch, Meritxell Martı́, Sialia Karina Mellink-Méndez, Luı́s Gales, Nuno G. Azóia, Ulyana Shimanovich, Artur Cavaco‐Paulo,
Synopsis Human hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also report the study of the interaction between those two main components of hair, ...
Tópico(s): Skin and Cellular Biology Research
2013 - Wiley | International Journal of Cosmetic Science
Agnes Stépán, George E Anasontzis, Teresa Matamá, Artur Cavaco‐Paulo, Lisbeth Olsson, Paul Gatenholm,
This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX films and that a cutinase from Fusarium solani pisi surface acetylates these films. The ...
Tópico(s): Enzyme Catalysis and Immobilization
2013 - Elsevier BV | Journal of Biotechnology
Carla Silva, Teresa Matamá, Nuno G. Azóia, Catarina Mansilha, Margarida Casal, Artur Cavaco‐Paulo,
The application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli, and the purified protein was biochemically characterized. The IC50 values (concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fragrances, namely, citronellol, benzyl benzoate, citronellyl valerate and ethyl valerate. ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2013 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Idalina Gonçalves, Madalena Martins, Ana Loureiro, Andreia C. Gomes, Artur Cavaco‐Paulo, Carla Silva,
The main goal of this work is to develop a novel and environmental-friendly technology for cotton bleaching with reduced processing costs. This work exploits a combined laccase-hydrogen peroxide process assisted by ultrasound. For this purpose, specific reactors were studied, namely ultrasonic power generator type K8 (850 kHz) and ultrasonic bath equipment Ultrasonic cleaner USC600TH (45 kHz). The optimal operating conditions for bleaching were chosen considering the highest levels of hydroxyl radical ...
Tópico(s): Dermatologic Treatments and Research
2013 - Elsevier BV | Ultrasonics Sonochemistry
Artur Ribeiro, Teresa Matamá, Célia F. Cruz, Andreia C. Gomes, Artur Cavaco‐Paulo,
The objective of this work was to develop a new strategy to physically 'repair' chemically damaged hair. Hence the human eye γD-crystallin, a protein from the superfamily characterized structurally by the Greek key motif, was studied. The human γD-crystallin was chosen based on the ability of proteins belonging to this superfamily to be involved in the coating of specific structures. Two crystallins were used on the study, the wild type (Protein Data Bank ID: 1HK0) and the mutant protein. The mutant ...
Tópico(s): melanin and skin pigmentation
2013 - Wiley | International Journal of Cosmetic Science
Ulyana Shimanovich, Gonçalo J. L. Bernardes, Tuomas P. J. Knowles, Artur Cavaco‐Paulo,
Micro- and nano-scale systems have emerged as important tools for developing clinically useful drug delivery systems. In this tutorial review, we discuss the exploitation of biomacromolecules for this purpose, focusing on proteins, polypeptides, nucleic acids and polysaccharides and mixtures thereof as potential building blocks for novel drug delivery systems. We focus on the mechanisms of formation of micro- and nano-scale protein-based capsules and shells, as well as on the functionalization of ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2013 - Royal Society of Chemistry | Chemical Society Reviews
Andreia Vasconcelos, Artur Cavaco‐Paulo,
Keratins are naturally derived proteins that can be fabricated into several biomaterials morphologies including films, sponges and hydrogels. As a physical matrix, keratin biomaterials have several advantages of both natural and synthetic materials that are useful in tissue engineering and controlled released applications. Like other naturally derived protein biomaterials, such as collagen, keratin possess amino acid sequences, similar to the ones found on extracellular matrix (ECM), that may interact ...
Tópico(s): Skin Protection and Aging
2013 - Bentham Science Publishers | Current Drug Targets
Produção Nacional
Revisado por pares
Daniela Gomes, Teresa Matamá, Artur Cavaco‐Paulo, Galba Maria de Campos‐Takaki, Alexandra A. Salgueiro,
Background:The hydrolytic action of cutinases has been applied to the degradation of plastics.Polyethylene terephthalate (PET) have long half-life which constitutes a major problem for their treatment as urban solid residues.The aim of this work was to characterize and to improve stable the enzyme to optimize the process of degradation using enzymatic hydrolysis of PET by recombinant cutinases. Results:The wild type form of cutinase from Fusarium solani pisi and its C-terminal fusion to cellulose ...
Tópico(s): Animal Genetics and Reproduction
2013 - Elsevier BV | Electronic Journal of Biotechnology
Eugénia Nogueira, Ana Loureiro, Patrícia Nogueira, Jaime Freitas, Catarina R. Almeida, Johan Härmark, Hans Hebert, Alexandra Moreira, Alexandre M. Carmo, Ana Preto, Andreia C. Gomes, Artur Cavaco‐Paulo,
Liposomes and protein based nanoparticles were tuned with different polymers and glycolipids to improve stealth and thus decrease their clearance by macrophages. Liposomes were coated with polyethylene glycol (PEG) and brain-tissue-derived monosialoganglioside (GM1). Bovine serum albumin (BSA) nanoparticles were produced incorporating a PEGylated surfactant (PEG-surfactant). All obtained nanoparticles were monodisperse, with sizes ranging from 80 to 120 nm, with a zeta-potential close to zero. The presented ...
Tópico(s): Characterization and Applications of Magnetic Nanoparticles
2013 - Royal Society of Chemistry | Faraday Discussions
Madalena Martins, Nuno G. Azóia, Artur Ribeiro, Ulyana Shimanovich, Carla Silva, Artur Cavaco‐Paulo,
Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.
Tópico(s): Proteins in Food Systems
2013 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Helena Ferreira, Teresa Matamá, Raquel Silva, Carla Silva, Andreia C. Gomes, Artur Cavaco‐Paulo,
From ancientness, suitable materials have been developed to cover the wounds in order to prevent infections and promote proper wound healing. In this study, the successful development of functionalized nonwoven gauzes with liposomes entrapping anti-inflammatory piroxicam is reported. Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) that can suppress a persistent inflammatory response, leading to improved wound healing. The results demonstrated that the highest NSAID concentration released ...
Tópico(s): Surgical Sutures and Adhesives
2013 - Elsevier BV | Reactive and Functional Polymers
Tarsila G. Castro, Manuel Melle‐Franco, Cristina E. A. Sousa, Artur Cavaco‐Paulo, João Carlos Marcos,
This review provides a fresh overview of non-canonical amino acids and their applications in the design of peptidomimetics. Non-canonical amino acids appear widely distributed in nature and are known to enhance the stability of specific secondary structures and/or biological function. Contrary to the ubiquitous DNA-encoded amino acids, the structure and function of these residues are not fully understood. Here, results from experimental and molecular modelling approaches are gathered to classify ...
Tópico(s): Antimicrobial Peptides and Activities
2023 - Multidisciplinary Digital Publishing Institute | Biomolecules
Xue Yang, Jing Zhao, Artur Cavaco‐Paulo, Jing Su, Hongbo Wang,
Laccase have received extensive attention in pollutant degradation, but its practical viability is largely affected by the poor stability, easy inactivation and difficulty in recycling for the present. Enzyme immobilization offers enhanced enzyme stability and constructs a synergistic system for the efficient adsorption and degradation of pollutants. In this study, bimetallic Cu/Zn ZIFs were synthesized by co-precipitation method as the protective carrier for laccase. Lac@Cu-ZIF-90 exhibited a good ...
Tópico(s): Nanomaterials for catalytic reactions
2023 - Elsevier BV | International Journal of Biological Macromolecules
David S. Freitas, Diana Rocha, Tarsila G. Castro, Jennifer Noro, Vânia I. B. Castro, Marta A. Teixeira, Rui L. Reis, Artur Cavaco‐Paulo, Carla Silva,
Quercus suber cork generates bioactive components, such as phenolic acids, terpenoids, and tannins, with excellent biological properties, including antioxidant, anti-inflammatory, and antiaging activities. Aiming to design environmentally benign processes to eliminate, or reduce, the use of toxic chemicals, we propose the green extraction of bioactives from cork using natural deep eutectic solvents (NADES). Several deep eutectic mixtures were developed, through the mixture of natural compounds, namely, ...
Tópico(s): Botanical Research and Applications
2022 - American Chemical Society | ACS Sustainable Chemistry & Engineering
Rachel E. Young, Samuel Hofbauer, Rachel Riley,
... Google Scholar, 10Guimaraes D. Noro J. Silva C. Cavaco-Paulo A. Nogueira E. Protective effect of saccharides on ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
2022 - Elsevier BV | Molecular Therapy
Cristiana Costa, Artur Cavaco‐Paulo, Teresa Matamá,
The importance of the hair follicle in the process of cutaneous drug penetration has been established since this skin appendage was recognized as an entry point for topically applied substances. A comprehensive review on the hair follicle as a target per se is here provided, exploring the current knowledge on both targeted regions and delivery systems that take advantage of this permeation route. The follicular penetration is a complex process, whose effectiveness and efficiency strongly depends ...
Tópico(s): Hair Growth and Disorders
2021 - Elsevier BV | International Journal of Pharmaceutics
Diana Guimarães, Artur Cavaco‐Paulo, Eugénia Nogueira,
Liposomes are spherical vesicles consisting of one or more concentric phospholipid bilayers enclosing an aqueous core. Being both nontoxic and biodegradable, liposomes represent a powerful delivery system for several drugs. They have improved the therapeutic efficacy of drugs through stabilizing compounds, overcoming obstacles to cellular and tissue uptake and increasing drug biodistribution to target sites in vivo, while minimizing systemic toxicity. This review offers an overview of liposomes, ...
Tópico(s): Advanced Drug Delivery Systems
2021 - Elsevier BV | International Journal of Pharmaceutics
Filipa Gonçalves, Artur Ribeiro, Carla Silva, Artur Cavaco‐Paulo,
The olfactory system of mammals allows the detection and discrimination of thousands of odors from the environment. In mammals, odorant-binding proteins (OBPs) are considered responsible to carry odorant molecules across the aqueous nasal mucus to the olfactory receptors (ORs). The three-dimensional structure of these proteins presents eight antiparallel β-sheets and a short α-helical segment close to the C terminus, typical of the lipocalins family. The great ability of OBPs to bind differentiated ...
Tópico(s): Neurobiology and Insect Physiology Research
2021 - Taylor & Francis | Critical Reviews in Biotechnology
The review focus on the effect of ultrasound on protein functionality. The presence of transient ultrasonic mechanical waves induce various sonochemical and sonomechanical effects on a protein. Sonochemical effects include the breakage of chains and/or the modification of side groups of aminoacids. Sonomechanical modifications by enhanced molecular agitation, might lead to the transient or permanent modification of the 3D structure of the folded protein. Since the biological function of proteins ...
Tópico(s): Microencapsulation and Drying Processes
2021 - Elsevier BV | Ultrasonics Sonochemistry
Ana Tinoco, Madalena Martins, Artur Cavaco‐Paulo, Artur Ribeiro,
Tópico(s): Animal Genetics and Reproduction
2021 - Elsevier BV | Trends in biotechnology
Yuwei Liu, Jing Su, Diana Carvalho, Jiajia Fu, Madalena Martins, Artur Cavaco‐Paulo,
The methylimidazolium chloride ionic liquid (IL) was successfully used for the dissolution of cotton cellulose. IL and IL-cellulose were investigated by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy analyses. The obtained IL-cellulose was applied to wool and hair keratin fiber surfaces, forming a fine coating layer. From FT-IR and XRD analyses, the results suggested the reduction of hydrogen bonds and crystallinity in IL-cellulose-coated wool fibers. This effect was caused ...
Tópico(s): Enzyme-mediated dye degradation
2021 - American Chemical Society | ACS Sustainable Chemistry & Engineering