Dmitry M. Hushpulian, Pavel A. Savitski, A. Rojkova, T. A. Chubar, V. A. Fechina, Ivan Yu. Sakharov, L. Mark Lagrimini, В. И. Тишков, Irina G. Gazaryan,
Tópico(s): Amino Acid Enzymes and Metabolism
2003 - Pleiades Publishing | Biochemistry (Moscow)
Ari Frank, Mikhail M. Savitski, Michael L. Nielsen, Roman A. Zubarev, Pavel A. Pevzner,
The recent proliferation of novel mass spectrometers such as Fourier transform, QTOF, and OrbiTrap marks a transition into the era of precision mass spectrometry, providing a 2 orders of magnitude boost to the mass resolution, as compared to low-precision ion-trap detectors. We investigate peptide de novo sequencing by precision mass spectrometry and explore some of the differences when compared to analysis of low-precision data. We demonstrate how the dramatically improved performance of de novo ...
Tópico(s): Genomics and Phylogenetic Studies
2006 - American Chemical Society | Journal of Proteome Research
Pavel Loiko, Vasili G. Savitski, Alan J. Kemp, A. A. Pavlyuk, Н. В. Кулешов, K. V. Yumashev,
The anisotropy of thermal lensing and the photo-elastic effect is characterized for diode-pumped Nd : KGd(WO4)2 crystals cut along the Np and Ng optical indicatrix axes and along its optical axis, O = Ng + 43°, at a laser wavelength of 1067 nm. Distortions in the spatial profile of the output laser beam are analyzed. The thermal lens is astigmatic; the orientation of its principal meridional planes, A and B, is determined by the anisotropy of photo-elastic effect. The thermal lens has opposite signs for ...
Tópico(s): Luminescence Properties of Advanced Materials
2014 - IOP Publishing | Laser Physics Letters
Dmitry M. Hushpulian, А. А. Полозников, Pavel A. Savitski, А. М. Рожкова, Tatyana A. Chubar, V. A. Fechina, Marina A. Orlova, В. И. Тишков, Irina G. Gazaryan, L. Mark Lagrimini,
The role of the conserved glutamic acid residue in anionic plant peroxidases with regard to substrate specificity and stability was examined. A Glu141Phe substitution was generated in tobacco anionic peroxidase (TOP) to mimic neutral plant peroxidases such as horseradish peroxidase C (HRP C). The newly constructed enzyme was compared to wild-type recombinant TOP and HRP C expressed in E. coli. The Glu141Phe substitution supports heme entrapment during the refolding procedure and increases the reactivation ...
Tópico(s): Lignin and Wood Chemistry
2007 - De Gruyter | Biological Chemistry
Vladimir Tomberg, Pjotr Savitski, Pavel Djundik, Vsevolods Berzinsh,
While testing students is a popular pedagogical evaluation approach in both formal and informal learning, there still is a lack of usable, interoperable and open source tools that could have enough motivating affordances for instructors. Another issue with the testing tools is a poor support of the question and test interoperability standards. Such standards exist for already seventeen years, however, the tools which support these standards are either big-scale proprietary products or are not easy ...
Tópico(s): Online Learning and Analytics
2017 - Springer Science+Business Media | Communications in computer and information science
Pavel Loiko, K. V. Yumashev, Н. В. Кулешов, Vasili G. Savitski, S. Calvez, D. Burns, A. A. Pavlyuk,
A comparative study of thermal lensing effect in diode laser pumped Ng- and Np-cut Nd:KGd(WO4)2 (KGW) laser crystals was performed for laser emission polarized along the principle refractive axis, Nm. The thermal lens in the Ng-cut Nd: KGW was found to be weakly astigmatic with a positive refractive power for both the Nm- and Np-directions. For Np -cut Nd:KGW, strong astigmatism was observed and the refractive powers in the Ng- and Nm-directions had opposing signs. The degree of astigmatism was found ...
Tópico(s): Laser Design and Applications
2009 - Optica Publishing Group | Optics Express
Dmitry M. Hushpulian, А. А. Полозников, Pavel A. Savitski, А. М. Рожкова, Tatyana A. Chubar, V. A. Fechina, L. Mark Lagrimini, В. И. Тишков, Irina G. Gazaryan,
The wild-type anionic tobacco peroxidase and its Glu141Phe mutant have been expressed in Escherichia coli, and reactivated to yield active enzymes. A Glu141Phe substitution was made with the tobacco anionic peroxidase (TOP) to mimic neutral plant peroxidases, such as horseradish peroxidase (HRP). Both recombinant forms of tobacco peroxidase show extremely high activity in luminol oxidation with hydrogen peroxide, and thus, preserve the unique property of the native tobacco peroxidase, a superior chemiluminescent ...
Tópico(s): Advanced Nanomaterials in Catalysis
2007 - Taylor & Francis | Biocatalysis and Biotransformation
Pavel Loiko, S. J. Yoon, Josep María Serres, Xavier Mateos, Stephen J. Beecher, Rolf B. Birch, Vasili G. Savitski, Alan J. Kemp, K. V. Yumashev, Uwe Griebner, Valentin Petrov, Magdalena Aguiló, Francesc Dı́az, J. I. Mackenzie,
High-resolution absorption and stimulated-emission cross-section spectra are presented for monoclinic Nd:KGd(WO4)2 (Nd:KGW) laser crystals in the temperature range 77–450 K. At room-temperature, the maximum stimulated emission cross-section is σSE = 21.4 × 10−20 cm2 at 1067.3 nm, for light polarization E || Nm. The lifetime of the 4F3/2 state of Nd3+ in KGW is practically temperature independent at 115 ± 5 μs. Measurement of the energy transfer upconversion parameter for a 3 at.% Nd:KGW crystal proved that ...
Tópico(s): Photorefractive and Nonlinear Optics
2016 - Elsevier BV | Optical Materials
Valentin Ivanov, Klaus Augsburg, Dzmitry Savitski, Jiří Plíhal, Pavel Nedoma, Jaroslav Machan,
The paper introduces the method of assessment of vehicle manoeuvres through a set of global cost functions. The corresponding cost functions can be derived for different domains like longitudinal and lateral dynamics, driving comfort and other. The procedures of the computation of the cost functions include in general: (1) Selection of vehicle dynamics parameters relevant to the domain; (2) Transformation the appointed parameter to the dimensionless form; (3) Definition of weighting factors for each ...
Tópico(s): Vehicle emissions and performance
2012 - Springer Science+Business Media | Lecture notes in electrical engineering
Irina G. Gazaryan, Pavel A. Savitski, A. Rojkova, Dmitry M. Hushpulian, T. A. Chubar, V. A. Fechina, Ivan Yu. Sakharov, В. И. Тишков, L. Mark Lagrimini,
Tópico(s): Enzyme Production and Characterization
2003 - American Chemical Society | Biochemistry
Sangtae Kim, Nikolai Mischerikow, Nuno Bandeira, J. Daniel Navarro, Louis Wich, Shabaz Mohammed, Albert J. R. Heck, Pavel A. Pevzner,
... peptides (20.Zubarev R.A. Zubarev A.R. Savitski M.M. Electron capture/transfer versus collisionally activated/ ... information (20.Zubarev R.A. Zubarev A.R. Savitski M.M. Electron capture/transfer versus collisionally activated/ ... Scopus (128) Google Scholar, 22.Nielsen M.L. Savitski M.M. Zubarev R.A. Improving protein identification ... Text PDF PubMed Scopus (130) Google Scholar, 23.Savitski M.M. Nielsen M.L. Kjeldsen F. Zubarev ... peptide sequencing than traditional CID-based algorithms (23.Savitski M.M. Nielsen M.L. Kjeldsen F. Zubarev ...
Tópico(s): Analytical Chemistry and Chromatography
2010 - Elsevier BV | Molecular & Cellular Proteomics
... Sasaki, Tomoyuki Gargiulo, Valentina Sauer, Karen Gaspar, Guilherme Savitski, Vasili Gatti, Carlo Scajev, Patrik Gauden, Piotr A. ... Elena Wińska, Katarzyna Korneva, Anna Witzel, Bernd Koroteev, Pavel S.Wolarz, Eryk Koshimizu, Masanori Wolny, Juliusz A. ...
Tópico(s): Inorganic Fluorides and Related Compounds
2020 - Multidisciplinary Digital Publishing Institute | Crystals