Lourdes Lledó, María Isabel Gegúndez, Consuelo Giménez-Pardo, Rufino Álamo, Pedro Fernández‐Soto, Maria Sofia Núncio, José Vicente Saz,
This paper reports a 17-year seroepidemiological surveillance study of Borrelia burgdorferi infection, performed with the aim of improving our knowledge of the epidemiology of this pathogen. Serum samples (1,179) from patients (623, stratified with respect to age, sex, season, area of residence and occupation) bitten by ticks in two regions of northern Spain were IFA-tested for B. burgdorferi antibodies. Positive results were confirmed by western blotting. Antibodies specific for B. burgdorferi were ...
Tópico(s): Vector-Borne Animal Diseases
2014 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Luz Cachulo, Rufino Silva, Pedro Fonseca, Isabel Pires, Santos Carvajal‐Gonzalez, Rui Bernardes, José Cunha‐Vaz,
To identify morphological and/or functional early markers of choroidal neovascularization (CNV) development in fellow eyes of patients with exudative age-related macular degeneration (AMD).This is a single-center, prospective, observational, longitudinal 2-year study.Patients were enrolled with the diagnosis of neovascular AMD in 1 eye and early age-related maculopathy (ARM) in the fellow eye. Intervention or Methods: All patients completed the baseline assessment and were followed up for up to 24 ...
Tópico(s): Glaucoma and retinal disorders
2010 - Karger Publishers | Ophthalmologica
João Figueira, Rufino Silva, José Henriques, Paulo Caldeira Rosa, Inês Laíns, Pedro Melo, Sandrina Nunes, José Cunha‐Vaz,
To compare the efficacy and safety of intravitreal ranibizumab (IVR) in monotherapy or associated with panretinal photocoagulation (PRP) versus conventional PRP, for high-risk proliferative diabetic retinopathy (PDR) without vitreoretinal traction.Multicenter randomized trial, with 3 treatment arms: PRP versus IVR alone and PRP + IVR combined treatment. Follow-up was performed at months 3, 6 and 12.Thirty-five subjects were randomized and 32 used for analysis. Complete regression of neovessels elsewhere ...
Tópico(s): Retinal and Optic Conditions
2015 - Karger Publishers | Ophthalmologica
Fernando J. Reyes‐Zurita, Eva E. Rufino‐Palomares, Pedro P. Medina, E. Leticia García-Salguero, Juan Peragón, Marta Cascante, José A. Lupiáñez,
We report that a novel triterpenoid, (2a,3b)-2,3-dihydroxyolean-12-en-28-oic acid (maslinic acid), isolated from olive pomace from Olea europaea, triggers primarily the extrinsic and later the intrinsic apoptotic pathways in Caco-2 human colon-cancer cells. Apoptosis induced by maslinic acid was confirmed by FACS analysis using annexine-V FICT staining. This induction of apoptosis was correlated with the early activation of caspase-8 and caspase-3, the activation of caspase-8 was also correlated with ...
Tópico(s): Phytochemical compounds biological activities
2013 - Elsevier BV | Biochimie
Eva E. Rufino‐Palomares, Fernando J. Reyes‐Zurita, Leticia García‐Salguero, Khalida Mokhtari, Pedro P. Medina, José A. Lupiáñez, Juan Peragón,
Maslinic acid (MA) is an anti-tumoural agent which shows potent anti-proliferative properties against the HT29 colon-cancer cells. To shed light upon the active mechanism of MA we have investigated its effects upon the cytoskeleton. We used a proteomics procedure based on two-dimensional gel electrophoresis, mass analysis and peptide mass fingerprinting. The incubation of HT29 cells with MA led to G1 cell-cycle arrest. After 24 hours' exposure to 3.7 μM (IC50/8) and 30 μM (IC50) MA fourteen differentially ...
Tópico(s): Natural product bioactivities and synthesis
2013 - Elsevier BV | Journal of Proteomics
Produção Nacional
Revisado por pares
Lívio César Cunha Nunes, Alexandre Bezerra Galindo, Annalu da Silva Oliveira de Deus, Daniel Dias Rufino Arcanjo, Karina Perrelli Randau, Haroudo Sátiro Xavier, Antônia Maria das Graças Lopes Citó, Pedró José Rolim Neto,
A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa ...
Tópico(s): Essential Oils and Antimicrobial Activity
2009 - Springer Science+Business Media | Revista Brasileira de Farmacognosia
José Luis Ruiz-Barba, Manuel Brenes, Rufino Jiménez-Dı́az, Pedro Garcı́a, António Garrido,
The characteristic phenolic content of NaOH‐treated and untreated olive brines is reported. Detection, quantification and isolation of the phenolic compounds were carried out by HPLC. Viability of Lactobacillus plantarum in the presence of single or combined fractions of these isolated compounds was tested. When assayed at the concentrations found in brines, only the single phenolic fraction containing hydroxytyrosol strongly inhibited Lact. plantarum. However, inhibition of Lact. plantarum was ...
Tópico(s): Microbial Metabolites in Food Biotechnology
1993 - Wiley | Journal of Applied Bacteriology
Fernando J. Reyes‐Zurita, Eva E. Rufino‐Palomares, Leticia García‐Salguero, Juan Peragón, Pedro P. Medina, Andrés Parra, Marta Cascante, José A. Lupiáñez,
Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t- ...
Tópico(s): Phytochemical compounds biological activities
2016 - Public Library of Science | PLoS ONE
Domingo Hernández, Margarita Rufino, Silvia Armas, Ana González, Pedro Gutiérrez, Pablo Cereceda Barbero, S. Vivancos, Concepción Rodríguez, José Rodríguez de Vera, Armando Torres,
Risk factors for surgical complications (SCs) following kidney transplantation in the modern transplant era need to be identified to perform appropriate prophylactic interventions.Records from 870 consecutive adult cadaveric kidney transplants done at a single centre were reviewed. SCs were classified into four groups: (i) vascular (12%, thrombosis or stenosis); (ii) haemorrhagic (12%); (iii) ureteral (7.5%, leaks and stenosis) and (iv) wound (16%, lymphocoeles or dehiscences).One or more SCs occurred in ...
Tópico(s): Neurological Complications and Syndromes
2006 - Oxford University Press | Nephrology Dialysis Transplantation
Carina Proença, Ana T. Rufino, José Miguel P. Ferreira de Oliveira, Marisa Freitas, Pedro Alexandrino Fernandes, Artur M. S. Silva, Eduarda Fernandes,
Type 2 diabetes (T2D) is the most common form of diabetes, and the number of people with this metabolic disease is steadily increasing worldwide. Among the available antidiabetic agents, α-glucosidase inhibitors are the most effective at reducing postprandial hyperglycaemia (PPHG), one of the main characteristics of T2D. However, most of the studies that have been performed have used the more readily available rat intestinal preparations or yeast α-glucosidase as the enzyme source, which despite being ...
Tópico(s): Diet, Metabolism, and Disease
2022 - Royal Society of Chemistry | Food & Function
Daniela Ribeiro, Adelaide Sousa, Pedro D. M. Nicola, José Miguel P. Ferreira de Oliveira, Ana T. Rufino, Marta Silva, Marisa Freitas, Félix Carvalho, Eduarda Fernandes,
Carotenoids are ubiquitously distributed in nature, β-carotene being the most frequently found carotenoid in the human diet. In the human body, β-carotene is absorbed, distributed and metabolized by enzymatic and/or non-enzymatic oxidant cleavage into several metabolites. Despite the broadly accepted biological value of β-carotene, it has also been considered a double-edged sword, mainly due to its potential antioxidant versus pro-oxidant behaviour. In this sense, the aim of this work was to scrutinize ...
Tópico(s): Retinoids in leukemia and cellular processes
2020 - Elsevier BV | Food and Chemical Toxicology
Produção Nacional
Revisado por pares
Danúbia da Cunha de Sá-Caputo, Cintia Renata Sousa Gonçalves, Danielle Soares Morel, Eloá Moreira Marconi, Patrícia Fróes, Rogério Rufino, Cláudia Henrique da Costa, Agnaldo José Lopes, Adriano Arnóbio, Nasser Ribeiro Asad, Pedro J. Marín, Trentham Furness, Mário Bernardo-Filho,
Background . Appropriate management, including pulmonary rehabilitation, associated with correct diagnosis of chronic obstructive pulmonary disease (COPD) in patients can contribute to improving clinical conditions of these patients. Physical activity is recommended for COPD patients. Whole-body vibration (WBV) is a modality of physical activity. Putting together the biological effects and safe use of WBV, it may be a potentially feasible intervention to add to pulmonary rehabilitation. The purpose ...
Tópico(s): Noise Effects and Management
2016 - Hindawi Publishing Corporation | Evidence-based Complementary and Alternative Medicine
Cláudia Farinha, Ana Luísa Silva, Rita Coimbra, Sandrina Nunes, Maria Luz Cachulo, João Pedro Marques, Isabel Pires, José Cunha‐Vaz, Rufino Silva,
Tópico(s): Glaucoma and retinal disorders
2021 - Springer Science+Business Media | Graefe s Archive for Clinical and Experimental Ophthalmology
Produção Nacional
Revisado por pares
Darne Germano de Almeida, Rita de Cássia F. Soares da Silva, Hugo Morais Meira, Pedro Pinto Ferreira Brasileiro, Elias José Silva, Juliana M. Luna, Raquel D. Rufino, Leonie Asfora Sarubbo,
Contamination by oil and its derivatives causes serious damage to the environment, motivating the development of innovative technologies for the removal of these contaminants, such as the use of biosurfactants. In the present study, the biosurfactant from Candida tropicalis UCP0996 produced in the low cost-medium formulated with molasses, residual frying oil, and corn steep liquor, was characterized and its toxicity, formulation, and application in removal and biodegradation of oil were investigated. ...
Tópico(s): Pesticide and Herbicide Environmental Studies
2021 - Multidisciplinary Digital Publishing Institute | Processes
Domingo Hernández, R Castaño Guerra, Antonio Milena, Armando Torres, Sagrario García, Candelaria García, Pedro Abreu, A Berenguer González, María Ángeles Ruiz Gómez, Margarita Rufino, José Manuel González‐Posada, Vı́ctor de Lorenzo, Eduardo Salido,
Background. Dietary fish oil, rich in omega‐3 polyunsaturated fatty acids, decreases TNF‐α, IL‐1β and IL‐2 levels, which may benefit renal transplant recipients. To explore this possibility, we studied the effect of fish oil on the incidence of acute rejection, in situ expression of interleukins (TNF‐α, IL‐1β and IL‐2) and renal function after renal transplantation. Methods. In a double‐blind clinical trial, 86 subjects with no immunological risk randomly received either 6 g/day of fish oil (fish ...
Tópico(s): Clinical Nutrition and Gastroenterology
2002 - Oxford University Press | Nephrology Dialysis Transplantation
João Pedro Marques, Ana Luísa Carvalho, José Henriques, Joaquim Murta, Jorge Saraiva, Rufino Silva,
The development of multicenter patient registries promotes the generation of scientific knowledge by using real-world data. A country-wide, web-based registry for inherited retinal dystrophies (IRDs) empowers patients and community organizations, while supporting formal partnerships research. We aim to describe the design, development and deployment of a country-wide, with investigators and stakeholders in the global aim to develop high-value, high-utility web-based, user-friendly and interoperable ...
Tópico(s): Retinal Diseases and Treatments
2020 - BioMed Central | Orphanet Journal of Rare Diseases
Khalida Mokhtari, Eva E. Rufino‐Palomares, Amalia Pérez‐Jiménez, Fernando J. Reyes‐Zurita, Celeny Figuera, Leticia García‐Salguero, Pedro P. Medina, Juan Peragón, José A. Lupiáñez,
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine ...
Tópico(s): Essential Oils and Antimicrobial Activity
2015 - Hindawi Publishing Corporation | Evidence-based Complementary and Alternative Medicine
M. Helena Vasconcelos, Inês Marques, Ana Rita Santos, Pedro Melo, Isabel Pires, João Figueira, Jose F. Abreu, Maria Luz Cachulo, Rufino Silva,
To evaluate morphological and functional chorioretinal changes 5 years after standard photodynamic therapy (PDT) for chronic central serous chorioretinopathy (CSC).A retrospective, nonrandomized study, including patients with chronic CSC treated with standard PDT and followed for at least 60 months. All patients underwent a complete ophthalmological examination, and the location and number of treatments were registered. Five or more years after treatment, subfoveal and non-subfoveal treated areas ...
Tópico(s): Ophthalmology and Visual Impairment Studies
2013 - Springer Science+Business Media | Graefe s Archive for Clinical and Experimental Ophthalmology
João Pedro Marques, José Filipe Costa, Marco Frederico Marques, Maria Luz Cachulo, João Figueira, Rufino Silva,
To assess and describe sequential morphological changes in the choroidal neovascularization (CNV) net using optical coherence tomography angiography (OCTA) in patients undergoing treatment with intravitreal antivascular endothelial growth factor (VEGF).Prospective cohort study. OCTA was performed sequentially: before (t0), 1 h (t1), 1 week (t2) and 1 month after the injection (t3), using Avanti RTVue XR equipped with the AngioVue® software (Optovue, Calif., USA). All images were classified by two independent ...
Tópico(s): Optical Coherence Tomography Applications
2016 - Karger Publishers | Ophthalmic Research
Eva E. Rufino‐Palomares, Amalia Pérez‐Jiménez, Fernando J. Reyes‐Zurita, Leticia García‐Salguero, Khalida Mokhtari, Antonio Herrera-Merchan, Pedro P. Medina, Juan Peragón, José A. Lupiáñez,
Pentacyclic triterpenes are natural substances, synthesized and present in variable amounts in a large number of terrestrial and aquatic plants, which act not only as antioxidants and antimicrobials but also as poisons, antibiotics, protease inhibitors, and so on. From the organic chemistry point of view, they are compounds derived from isoprene. These compounds consist of 30 carbons with different substituents, which facilitate their chemical identification and confer different physico-chemical ...
Tópico(s): Biological Activity of Diterpenoids and Biflavonoids
2015 - Bentham Science Publishers | Current Organic Chemistry
Amalia Pérez‐Jiménez, Eva E. Rufino‐Palomares, Nieves Fernández‐Gallego, María del Carmen Ortuño‐Costela, Fernando J. Reyes‐Zurita, Juan Peragón, Leticia García‐Salguero, Khalida Mokhtari, Pedro P. Medina, José A. Lupiáñez,
Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product with demonstrated pharmacological functions including anti-inflammation, anti-tumor and anti-oxidation, among others.Here we report the effects of MA on the adipogenesis process in 3T3-L1 cells.Cell viability, glucose uptake, cytoplasmic triglyceride ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2016 - Elsevier BV | Phytomedicine
Cláudia Farinha, Torcato Santos, Inês Marques, João Pedro Marques, Luísa Ribeiro, João Figueira, Rufino Silva, José Cunha‐Vaz,
To test OCT-Leakage, a new method of analyzing and mapping sites of lower optical reflectivity found on OCT, by examining eyes with various types of retinal edema to identify abnormal increases in retinal extracellular fluid.Prospective analysis of a cohort of cases.Healthy eyes and eyes with retinal edema in the setting of different retinal diseases.Prospective OCT-Leakage analysis of 12 eyes with various types of retinal edema, such as diabetic macular edema, branch retinal vein occlusion, idiopathic ...
Tópico(s): Retinal Diseases and Treatments
2017 - Elsevier BV | Ophthalmology Retina
Fernando J. Reyes‐Zurita, Marta Medina‐O’Donnell, Rosa M. Ferrer‐Martín, Eva E. Rufino‐Palomares, Samuel Martin-Fonseca, Francisco Rivas, Antonio Martı́nez, Andrés Garcı́a-Granados, Amalia Pérez‐Jiménez, Leticia García‐Salguero, Juan Peragón, Khalida Mokhtari, Pedro P. Medina, Andrés Parra, José A. Lupiáñez,
Antiproliferative and proapoptotic effects of 3- O -succinyl-28- O -benzyl oleanolate on B16–F10 skin-melanoma cells.
Tópico(s): Genomics, phytochemicals, and oxidative stress
2016 - Royal Society of Chemistry | RSC Advances
José Felipe Orzuna‐Orzuna, Griselda Dorantes-Iturbide, Alejandro Lara-Bueno, Germán David Mendoza Martínez, Luis Alberto Miranda‐Romero, Rufino López‐Ordaz, Pedro Abel Hernández-García,
The objective of this study was to evaluate the effects of dietary supplementation of a polyherbal mixture (HM) containing saponins, flavonoids, and polysaccharides on productive performance, carcass characteristics and meat quality of lambs during the final fattening period. Thirty-six Dorper × Katahdin lambs (23.27 ± 1.23 kg body weight (BW)) were housed in individual pens and were assigned to four treatments (n = 9) with different doses of HM: 0 (CON), 1 (HM1), 2 (HM2) and 3 (HM3) g of HM kg−1 of DM for 56 ...
Tópico(s): Ruminant Nutrition and Digestive Physiology
2021 - Multidisciplinary Digital Publishing Institute | Agriculture
Rufino López‐Ordaz, Claudio Vite-Cristóbal, José Guadalupe García-Muñiz, Pedro Martínez‐Hernández,
El objetivo del presente estudio fue evaluar los factores ambientales y la proporción óptima de genes Bos taurus (BT) y su influencia en la producción de leche (PL) y el comportamiento reproductivo de bovinos cruzados ¾ Holstein (H) x ¼ Cebú (C); 3H1C, ¾ Suizo (S) x ¼ C; 3S1C, 1/2H x 1/2C; HC y 1/2S x 1/2C; SC. La información analizada se obtuvo de 276 lactancias de 69 vacas de los grupos raciales de tres hatos en Veracruz, México. Las variables estudiadas incluyeron la PL por lactancia (PTL), por vaca/ ...
Tópico(s): Natural Products and Biological Research
2008 - University of Córdoba | Archivos de Zootecnia
João Figueira, David Martins, Bernardete Pessoa, Natália Ferreira, Angelina Meireles, António Sampaio Soares, Ângela Carneiro, F Vaz, João Nascimento, Natacha Moreno, José Roque, Manuel Domingues, Rita Flores, Carla Teixeira, Eduardo Conde, Filipe Henriques, Helena Proença, José Pita Negrão, Marisa Barbosa, Rufino Silva, Sara Vaz-Pereira, Pedro Neves, Nuno Gomes, Miguel Raimundo,
<b><i>Aim:</i></b> Evaluate the real-life experience with ocriplasmin on vitreomacular traction (VMT) release and full-thickness macular hole (FTMH) closure in Portugal. <b><i>Methods:</i></b> Multicentric, retrospective study of 83 eyes of 78 patients who were treated with intravitreal ocriplasmin for VMT with and without FTMH. Primary outcomes were VMT release and FTMH closure. Secondary outcomes included visual acuity changes and structural features on ...
Tópico(s): Vascular Malformations Diagnosis and Treatment
2016 - Karger Publishers | Ophthalmic Research
Pedro Abel Hernández-García, Alejandro Lara-Bueno, Germán David Mendoza Martínez, José R. Bárcena-Gama, Fernando Xicoténcatl Plata Pérez, Rufino López‐Ordaz, Jose A. Martinez,
Yeasts and organic minerals are used in diets to improve health, productive performance and some carcass characteristics of ruminants and non-ruminants. Thirty-two lambs (Pelibuey×Katahdin; BW=(30.55±1.67) kg; n=8) were used in a 56-d feeding experiment to study the effects of different levels of live yeast (Saccharomyces cerevisiae; yeast), selenium (Se) and chromium (Cr) mixed (Se-Cr), and a mixture of yeast-Se-Cr on growth performance and carcass traits. Animals were stratified by body weight (BW) ...
Tópico(s): Meat and Animal Product Quality
2015 - Elsevier BV | Journal of Integrative Agriculture
Cláudia Farinha, Maria Luz Cachulo, Dalila Alves, Isabel Pires, João Pedro Marques, Patrícia Barreto, Sandrina Nunes, José Coutinho Costa, Amélia Martins, Isa Sobral, Inês Laíns, João Figueira, Luísa Ribeiro, José Cunha‐Vaz, Rufino Silva,
To describe the 6.5-year incidence and progression of age-related macular degeneration (AMD) in a coastal town of central Portugal.Population-based cohort study. Participants underwent standardized interviews and ophthalmological examination. Color fundus photographs were graded according to the International Classification and Grading System for AMD and ARM. The crude and age-standardized incidence of early and late AMD was calculated, and progression was analyzed.The 6.5-year cumulative incidence ...
Tópico(s): Glaucoma and retinal disorders
2019 - Karger Publishers | Ophthalmic Research
Maria Luz Cachulo, Inês Laíns, Conceição Lobo, João Figueira, Luísa Ribeiro, João Pedro Marques, José Coutinho Costa, António Vieira, João Rodrigues, Dalila Alves, Sandrina Nunes, Miguel Costa, Vítor Rodrigues, José Cunha‐Vaz, Cécile Delcourt, Rufino Silva,
Abstract Purpose To determine the age‐ and sex‐specific prevalence of early and late age‐related macular degeneration ( AMD ) in two P ortuguese population‐based samples and to identify its risk factors. Population A population of 6023 adults aged ≥55 years was recruited from two P ortuguese primary healthcare units in the central region of P ortugal – one from a coastal ( n = 3000) and another from an inland town ( n = 3023). Methods Cross‐sectional population‐based study. Participants were enrolled in the two ...
Tópico(s): Glaucoma and retinal disorders
2016 - Wiley | Acta Ophthalmologica
João Pedro Marques, Sara Vaz-Pereira, José Costa, Ana Marta, José Henriques, Rufino Silva,
Abstract Rare disease registries increase research accessibility for patients, while providing clinicians/investigators with a coherent data ecosystem necessary to boost research and patient care. The IRD-PT registry is a national, web-based, interoperable registry for inherited retinal degenerations (IRDs) designed to generate scientific knowledge and collect high-quality data on the epidemiology, genomic landscape and natural history of IRDs in Portugal. In two years, the number of enrolled patients ...
Tópico(s): Genomic variations and chromosomal abnormalities
2022 - BioMed Central | Orphanet Journal of Rare Diseases