Aditi Gupta, Kamisetty Krishna Rao, Umakant Sahu, Pundi N. Rangarajan,
... global regulator of multiple metabolic pathways of P. pastoris. Pichia pastoris (a.k.a. Komagataella phaffii) is a methylotrophic ... M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris.FEMS Microbiol. Rev. 2000; 24: 45-66Crossref PubMed ... Steiger M.G. Mattanovich D. Metabolic engineering of Pichia pastoris.Metab. Eng. 2018; 50: 2-15Crossref PubMed Scopus ( ... expression regulator 1 (Mxr1p) in the methylotrophic yeast Pichia pastoris.J. Biol. Chem. 2016; 291: 3648-3657Abstract Full ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2021 - Elsevier BV | Journal of Biological Chemistry
Dauenpen Meesapyodsuk, Yan Chen, Siew Hon Ng, Jianan Chen, Xiao Qiu,
... a highly active endogenous Δ12 desaturase in P. pastoris. Pichia Δ12 desaturase is a membrane-associated enzyme catalyzing ... the substrate level for the hydroxylation, the endogenous Pichia Δ12 desaturase gene was disrupted by homologous recombination in a haploid P. pastoris strain GS115. The disruption (D12-KO) was confirmed ...
Tópico(s): Enzyme Catalysis and Immobilization
2015 - Elsevier BV | Journal of Lipid Research
Haifa Chahed, Mohamed Boumaiza, Aymen Ezzine, Mohamed Néjib Marzouki,
... recombinant plasmid pPICZαA was used to transform Pichia pastoris. Pichia culture supernatants expressing the recombinant Endo2 (rEndo2) were ...
Tópico(s): Polysaccharides and Plant Cell Walls
2017 - Elsevier BV | International Journal of Biological Macromolecules
Agnetha Gustafsson, Magnus Sjöblom, Lena Strindelius, Tomas Johansson, Tilly Fleckenstein, Nathalie Chatzissavidou, Lene Lindberg, Jonas Ångström, Ulrika Rova, Jan Holgersson,
... glycans were stably expressed in the yeast Pichia pastoris. Pichia pastoris-produced PSGL-1/mIgG2b was shown to carry ...
Tópico(s): Immunotherapy and Immune Responses
2011 - Oxford University Press | Glycobiology
Jian-Lu An, Weixin Zhang, Weiping Wu, Guanjun Chen, Weifeng Liu,
... achieved when ReGal2 was expressed in an engineered Pastoris pichia expression system with a modified AOX1 promoter and ...
Tópico(s): Biofuel production and bioconversion
2019 - BioMed Central | Microbial Cell Factories
Praveen Kumar Agarwal, Vanita Uppada, Santosh Noronha,
Tópico(s): Amino Acid Enzymes and Metabolism
2013 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Sidong Liu, Thomas J. Charlesworth, John V. Bason, M.G. Montgomery, Michael E. Harbour, Ian M. Fearnley, John E. Walker,
... mitochondria of the fungal species Yarrowia lipolytica, Pichia pastoris, Pichia angusta and Saccharomyces cerevisiae. The subunit compositions of ...
Tópico(s): Fuel Cells and Related Materials
2015 - Portland Press | Biochemical Journal
Haruhiko Ehara, Takashi Umehara, Shun‐ichi Sekine, Shigeyuki Yokoyama,
... of Pol II from the methylotropic yeast Komagataella pastoris (Pichia pastoris) was determined. While the structure is highly homologous ...
Tópico(s): Enzyme Structure and Function
2017 - Elsevier BV | Biochemical and Biophysical Research Communications
Caroline Benedicte Kjærulff Mathiesen, Michael C. Carlsson, Stéphanie Brand, Svenning Rune Møller, Manja Idorn, Per thor Straten, Anders Elm Pedersen, Sally Dabelsteen, Adnan Halim, Peter Adler Würtzen, Jens Brimnes, Henrik Ipsen, Bent Larsen Petersen, Hans H. Wandall,
... α1-2 O-linked mannose residues from P pastoris (Pichia), or O-GalNAc residues from COSMC knockout CHO ...
Tópico(s): Immunotherapy and Immune Responses
2018 - Elsevier BV | Journal of Allergy and Clinical Immunology
... PECS Periplasmic expression cytometric screening; PK Pharmacokinetics; P. pastoris Pichia pastoris; PTM Post-translational modification; Rg Radius of gyration; ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2022 - Landes Bioscience | mAbs
Matthias Mack, Marion Wannemacher, Birgit Hobl, Patricia Pietschmann, Björn Hock,
The methylotrophic yeasts Pichia pastoris and Pichia angusta (Hansenula polymorpha) were used for the comparative heterologous production of two model mammalian proteins of pharmaceutical interest, the NK1- ... chromosomally integrated heterologous genes was found. In P. pastoris strains less degradation of the two model recombinant proteins was observed. Altogether, this paper provides a structured protocol for rapidly identifying productive Pichia strains for the synthesis of full-length recombinant ...
Tópico(s): Cancer-related gene regulation
2009 - Elsevier BV | Protein Expression and Purification
Chang Ting Tsai, Ching‐Tsan Huang,
... rumen fungus Neocallimastix frontalis was expressed in Pichia pastoris and Pichia methanolica. Using a complex medium with medium replacement before induction and the maintenance of the methanol induction level at 0.5%, P. pastoris was able to produce about 5400 U/ml ...
Tópico(s): Phytase and its Applications
2008 - Elsevier BV | Enzyme and Microbial Technology
Raúl A. Poutou‐Piñales, HA Córdoba-Ruiz, LA Barrera-Avellaneda, JM Delgado-Boada,
Pichia pastoris and Pichia methanolica have been used as expression systems for the production of recombinant protein. The main problems of ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2010 - Academic Journals | AFRICAN JOURNAL OF BIOTECHNOLOGY
Huan Chen, Cui Cao, Anna Kulinich, Li Liu, Yong‐Sam Jung, Josef Voglmeir,
... of mutant gene libraries. Keywords: Expression vector, Pichia pastoris, Pichia-specific autonomous replication sequence, promoter, red fluorescent protein, ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2017 - Bentham Science Publishers | Combinatorial Chemistry & High Throughput Screening
Produção Nacional
Revisado por pares
Giana Carla Gaboardi, Débora Aline Alves, Diego Gil de los Santos, Eduardo Gonçalves Xavier, Ana Paula Nunes, Paula Fonseca Finger, Emili Griep, Victor Fernando Büttow Roll, Patrícia Diaz de Oliveira, Arthur Silva, Ângela Nunes Moreira, Fabrício Rochedo Conceição,
... T1 (Control) received a basal diet without P. pastoris ; T2 ( Pichia Effluent) received a basal diet supplemented with P. ... conclusion, quail’s immune response was improved by Pichia pastoris X-33, either it was grown in YPD ... industrial residues, and the egg weight increased with Pichia pastoris X-33 grown in YPD, thereby demonstrating to ...
Tópico(s): Moringa oleifera research and applications
2019 - Nature Portfolio | Scientific Reports
The methylotrophic yeast Komagataella pastoris (syn. Pichia pastoris) is one of the few known yeasts that can utilize sulfamate ( $${\text{NH}}_{2} {\text{SO}}_{3}^{ - }$$ ) as a sulfur source. The ...
Tópico(s): Polyamine Metabolism and Applications
2017 - Springer Science+Business Media | Current Microbiology
Takeshi Sakaki, Ulrich Zähringer, Dirk Warnecke, Annette Fahl, Wolfgang Knogge, Ernst Heinz,
... in the three yeast species Candida albicans , Pichia pastoris and Pichia anomala , as well as in the six fungal species Sordaria macrospora , Pyrenophora teres, Ustilago maydis , Acremonium chrysogenum , Penicillium olsonii and Rhynchosporium secalis . Cerebroside was found in all organisms tested, whereas acylated sterol glycosides and glycosyldiacylglycerols were not found in any organism. Sterol glycosides were detected in P. pastoris strain GS115, U. maydis , S. macrospora and R. ...
Tópico(s): Fungal Biology and Applications
2001 - Wiley | Yeast
... Bagadi Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia ...
Tópico(s): Marine Biology and Environmental Chemistry
1943 - University of Chicago Press | The Quarterly Review of Biology
... E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG ...
Tópico(s): Enzyme function and inhibition
1960 - Elsevier BV | Biochimica et Biophysica Acta
... Evaluation of Soy Leghemoglobin Protein Preparation Derived From Pichia pastoris , Intended for Use as a Flavor Catalyst in ...
Tópico(s): Biochemical Analysis and Sensing Techniques
1939 - American Physiological Society | American Journal of Physiology-Legacy Content
R. A. Brierley, Christopher M. Bussineau, Ryszard Kosson, Anthony E. Melton, Robert S. Siegel,
... 1 p. 350-362 Fermentation Development of Recombinant Pichia pastoris Expressing the Heterologous Gene: Bovine Lysozyme R. A. ... efficient assembly of hepatitis B surface antigen in Pichia pastoris. Bio/Technology 5: 479–485. 2 Digan, M. ... Secretion of heterologous proteins from the methylotrophic yeast, Pichia pastoris. Dev. Ind. Microbiol. 29: 59–69. 3 Digan, ... of bovine lysozyme via secretion from the yeast, Pichia pastoris. In preparation. 4 Tschopp, J. F., G. Sverlow, ... secretion of glycosylated invertase in the methylotrophic yeast Pichia pastoris. Bio/Technology 5: 1305–1308. 5 Ellis, S. ...
Tópico(s): Polyamine Metabolism and Applications
1990 - Wiley | Annals of the New York Academy of Sciences
Zhengbing Jiang, Bei Gao, Ren Ren, Xingyi Tao, Yushu Ma, Dongzhi Wei,
... surface spontaneously, regarded as immobilized enzymes. Results Two Pichia pastoris cell surface display vectors based on the flocculation ... with the FLO gene was successfully transformed into Pichia pastoris KM71. The lipase LipB52 was expressed under the control of the AOX1 promoter and displayed on Pichia pastoris KM71 cell surface with the two Pichia pastoris cell surface display vectors. Localization of the displayed ... scanning microscopy (CLSM). The LipB52 displayed on the Pichia pastoris cell surface exhibited activity toward p -nitrophenol ester ...
Tópico(s): Electrochemical sensors and biosensors
2008 - BioMed Central | BMC Biotechnology
Joan Lin‐Cereghino, William W. Wong, See Xiong, W. Giang, Linda T. Luong, Jane Vu, Sabrina D. Johnson, Geoff P. Lin‐Cereghino,
... favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail The methylotrophic yeast Pichia pastoris has gained widespread acceptance as a system of ... 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45–66.Crossref, Medline, ... A.Y. Hessler, and K.R. Madden. 1985. Pichia pastoris as a host system for transformations. Mol. Cell. ... genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous ...
Tópico(s): Microbial metabolism and enzyme function
2005 - Future Science Ltd | BioTechniques
Oliver Spadiut, Simona Capone, Florian Krainer, Anton Glieder, Christoph Herwig,
... antibody and antibody fragment production, namely Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. Monoclonal antibodies (mAbs) and antibody ... antibody and antibody fragment production, namely Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. IntroductionOver the past three decades, ... notification concerning BD16449 – phospholipase C enzyme preparation from Pichia pastoris, http://www.accessdata.fda.gov/scripts/fcn/gras_ ... pathway [27Naatsaari L. et al.Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene ...
Tópico(s): Transgenic Plants and Applications
2013 - Elsevier BV | Trends in biotechnology
Umakant Sahu, Vinod Rajendra, Shankar S. Kapnoor, Raghu Bhagavat, Nagasuma Chandra, Pundi N. Rangarajan,
... that MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic in Saccharomyces ... that MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic in Saccharomyces ... Cloning and identification of methionine synthase gene from Pichia pastoris.Acta Biochim. Biophys. Sin. 2005; 37: 371-378Crossref ... stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as ζ crystallin.Nucleic Acids ... a master regulator of methanol utilization pathway in Pichia pastoris.Biochim. Biophys. Acta. 2009; 1789: 460-468Crossref PubMed ...
Tópico(s): Folate and B Vitamins Research
2017 - Elsevier BV | Journal of Biological Chemistry
The heterologous protein expression system of Pichia pastoris is now widely used for expression of many human proteins, because the efficiently expressed proteins will be correctly folded in Pichia pastoris cells and also efficiently secreted from the cells. ... human serum albumin (rHSA) is efficiently secreted from Pichia pastoris. Nowadays, the expression of rHSA exceeds 10g in ... protein is completely purified. Recombinant HSA expressed in Pichia pastoris was approved as a medicine by the authorities ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2010 - Bentham Science Publishers | Recent Patents on Biotechnology
Anneli Wennman, Ernst H. Oliw,
... were poor substrates. 9S-LOX was expressed in Pichia pastoris. Recombinant 9S-LOX oxidized 18:2n-6 directly ... were poor substrates. 9S-LOX was expressed in Pichia pastoris. Recombinant 9S-LOX oxidized 18:2n-6 directly ... Oliw E.H. Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands. ... we expressed 9S-LOX of M. salvinii in Pichia pastoris. The recombinant enzyme was found to oxidize 18: ... 2 mm; 5 µm, Kromasil) from ChromTech. pPICZαA, Pichia pastoris (strain X-33), phleomycin (Zeocin), and yeast nitrogen ...
Tópico(s): Biochemical and biochemical processes
2012 - Elsevier BV | Journal of Lipid Research
Stefan Linder, Manfred Schliwa, Eckhard Kube-Granderath,
... Vol. 73, No. 5Transformation of pPICZaA-E2 to Pichia pastoris X-33 and Mut+ phenotype analysisPsAA9A, a C1- ... Applied Microbiology and Biotechnology, Vol. 104, No. 22Engineered Pichia pastoris production of fusaruside, a selective immunomodulator17 June 2019 | ... Vectors, Vol. 7, No. 1Enhancing precursors availability in Pichia pastoris for the overproduction of S-adenosyl-l-methionine ... peak expression of hepatitis B surface antigen in Pichia pastoris shake flask systemOpen Life Sciences, Vol. 9, No. ... Vol. 129, No. 4High-level constitutive expression in Pichia pastoris and one-step purification of phospholipase D from ...
Tópico(s): Plant-Microbe Interactions and Immunity
1996 - Future Science Ltd | BioTechniques
... Oliw E.H. Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands. ... Oliw E.H. Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands. ... Oliw E.H. Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands. ... Oliw E.H. Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands. ... N466L, and Mn-LOX S469A were expressed in Pichia pastoris (strain X-33) as secreted proteins using the ...
Tópico(s): Biochemical and biochemical processes
2007 - Elsevier BV | Journal of Lipid Research
Michael A. Romanos, Carol A. Scorer, Jeffrey J. Clare,
... level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integratons of the gene. ... growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205– ... Baratti, J. (1980). Oxidation of methanol bythe yeast Pichia Pastoris. Purfication and properties of alcohol oxidase. Agric. Biol. ... Hessler, A. Y. and Madden, K. R. (1985). Pichia pastoris as a host system for transformations. Mol. Cell. ... and construction of methanol-utilisation-defective mutants of Pichia pastoris by gene disruption. In G. G. Stewart, I. ...
Tópico(s): RNA and protein synthesis mechanisms
1992 - Wiley | Yeast