... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
R. Kania, Gerda E. M. Lamers, Marcel J. Vonk, Esmee Dorpmans, Joyce A. Struik, Patrice Tran Ba Huy, Pieter S. Hiemstra, Guido V. Bloemberg, J. J. Grote,
To demonstrate the presence of mucosal biofilm in adenoid tissue using double staining for visualization of both the bacterial matrix and the bacterial cells. To identify bacterial species present on the surface of the studied adenoids.Prospective study.A total of 39 specimens of adenoidectomy were removed from children with chronic and/or recurrent otitis media. The specimens were prepared for light microscopy using Gram staining, scanning electron microscopy (SEM) and confocal laser scanning microscopy ( ...
Tópico(s): Nosocomial Infections in ICU
2008 - Wiley | The Laryngoscope
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
R. Kania, Gerda E. M. Lamers, Marcel J. Vonk, Patrice Tran Ba Huy, Pieter S. Hiemstra, Guido V. Bloemberg, J. J. Grote,
Objectives To demonstrate mucosal biofilms in human tissue by direct visualization of bacteria and glycocalyx using confocal laser scanning microscopy with double fluorescent staining on tonsils and to compare the findings with the results of scanning electron microscopy analysis. Design Prospective study. Setting Tertiary university-based referral center. Patients Twenty-four tonsils were obtained from children with chronic or recurrent tonsillitis. Interventions Tonsils were prepared for analysis by scanning ...
Tópico(s): Otolaryngology and Infectious Diseases
2007 - American Medical Association | Archives of Otolaryngology - Head and Neck Surgery
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Lisenka E.L.M. Vissers, Conny M.A. van Ravenswaaij, R.J.C. Admiraal, Jane A. Hurst, Bert B.A. de Vries, Irene M. Janssen, W A van der Vliet, Erik Huys, Pieter J. de Jong, Ben C.J. Hamel, Eric Schoenmakers, Han G. Brunner, Joris A. Veltman, Ad Geurts van Kessel,
CHARGE syndrome is a common cause of congenital anomalies affecting several tissues in a nonrandom fashion. We report a 2.3-Mb de novo overlapping microdeletion on chromosome 8q12 identified by array comparative genomic hybridization in two individuals with CHARGE syndrome. Sequence analysis of genes located in this region detected mutations in the gene CHD7 in 10 of 17 individuals with CHARGE syndrome without microdeletions, accounting for the disease in most affected individuals.
Tópico(s): Congenital heart defects research
2004 - Nature Portfolio | Nature Genetics
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Evelien Moorkens, Arnold G. Vulto, Isabelle Huys, Pieter Dylst, Brian Godman, Simon Keuerleber, Barbara Claus, Maria Dimitrova, Guenka Petrova, Ljiljana Sović-Brkičić, Juraj Slabý, Robin Šebesta, Ott Laius, Allan Karr, M. Beck, Jaana E. Martikainen, Gisbert Selke, Susan Spillane, Laura McCullagh, Gianluca Trifirò, Patricia Vella Bonanno, Asbjørn Mack, Antra Fogele, Anita Viksna, M Wladysiuk, Hélder Mota‐Filipe, Dmitry Meshkov, Marija Kalaba, Simona Mencej Bedrač, Jurij Fürst, Corrine Zara, Peter Skiöld, Einar Magnússon, Steven Simoens,
Background Across European countries, differences exist in biosimilar policies, leading to variations in uptake of biosimilars and divergences in savings all over Europe. Objectives The aim of this article is to provide an overview of different initiatives and policies that may influence the uptake of biosimilars in different European countries. Recommendations will be formulated on how to create sustainable uptake. Methods An overview of policies on biosimilars was obtained via a questionnaire, ...
Tópico(s): Health Systems, Economic Evaluations, Quality of Life
2017 - Public Library of Science | PLoS ONE
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
R. Kania, Gerda E. M. Lamers, Nicole van de Laar, Marloes Dijkhuizen, Ellen Lagendijk, Patrice Tran Ba Huy, Philippe Herman, Pieter S. Hiemstra, J. J. Grote, Johan H. M. Frijns, Guido V. Bloemberg,
The aim of this study was to demonstrate the presence of yeast and bacterial biofilms on the surface of tracheoesophageal voice prostheses (TVPs) by a double-staining technique with confocal laser scanning microscopy (CLSM). Biofilms of 12 removed TVPs were visualized by scanning electron microscopy, then stained with ConA-FITC and propidium iodide for CLSM. Microbial identification was by partial 16S rRNA gene analysis and ITS-2 sequence analysis. Microbial biofilms on the TVPs consisted of bacteria ...
Tópico(s): Antimicrobial Peptides and Activities
2010 - Taylor & Francis | Biofouling
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Pieter-Jan D’Huys, Ivan Lule, Sven Van Hove, Dominique Vercammen, Carine Wouters, Kristel Bernaerts, Jozef Anné, Jan Van Impe,
Streptomyces lividans is considered an interesting host for the secretory production of heterologous proteins. To obtain a good secretion yield of heterologous proteins, the availability of suitable nitrogen sources in the medium is required. Often, undefined mixtures of amino acids are used to improve protein yields. However, the understanding of amino acid utilization as well as their contribution to the heterologous protein synthesis is poor. In this paper, amino acid utilization by wild type ...
Tópico(s): Biochemical and Molecular Research
2010 - Elsevier BV | Journal of Biotechnology
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Philip Breugelmans, Pieter-Jan D’Huys, René Mot, Dirk Springael,
Linuron-mineralizing cultures were enriched from two linuron-treated agricultural soils in the presence and absence of a solid support. The cultures contained linuron-degrading bacteria, which coexisted with bacteria degrading either 3,4-dichloroaniline (3,4-DCA) or N, O-dimethylhydroxylamine (N, O-DMHA), two common metabolites in the linuron degradation pathway. For one soil, the presence of a solid support enriched for linuron-degrading strains phylogenetically related to but different from those ...
Tópico(s): Microbial Fuel Cells and Bioremediation
2007 - Oxford University Press | FEMS Microbiology Ecology
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Pieter-Jan D’Huys, Ivan Lule, Dominique Vercammen, Jozef Anné, Jan Van Impe, Kristel Bernaerts,
Constraint-based metabolic modeling comprises various excellent tools to assess experimentally observed phenotypic behavior of micro-organisms in terms of intracellular metabolic fluxes. In combination with genome-scale metabolic networks, micro-organisms can be investigated in much more detail and under more complex environmental conditions. Although complex media are ubiquitously applied in industrial fermentations and are often a prerequisite for high protein secretion yields, such multi-component ...
Tópico(s): Biofuel production and bioconversion
2012 - Elsevier BV | Journal of Biotechnology
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Jeroen Bouvin, Simon Cajot, Pieter-Jan D’Huys, Jerry Ampofo‐Asiama, Jozef Anné, Jan Van Impe, Annemie Geeraerd, Kristel Bernaerts,
13C-based metabolic flux analysis is an excellent technique to resolve fluxes in the central carbon metabolism but costs can be significant when using specialized tracers. This work presents a framework for cost-effective design of 13C-tracer experiments, illustrated on two different networks. Linear and non-linear optimal input mixtures are computed for networks for Streptomyces lividans and a carcinoma cell line. If only glucose tracers are considered as labeled substrate for a carcinoma cell ...
Tópico(s): Bioinformatics and Genomic Networks
2015 - Elsevier BV | Mathematical Biosciences
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Paul Janssen, R. Coopman, Geert Huys, Jean Swings, Maria Bleeker, Pieter Vos, Marc Zabeau, K. Kersters,
We investigated the usefulness of a novel DNA fingerprinting technique, AFLP, which is based on the selective amplification of genomic restriction fragments by PCR, to differentiate bacterial strains at the subgeneric level. In total, 147 bacterial strains were subjected to AFLP fingerprinting: 36 Xanthomonas strains, including 23 pathovars of Xanthomonas axonopodis and six pathovars of Xanthomonas vasicola, one strain of Stenotrophomonas, 90 genotypically characterized strains comprising all 14 ...
Tópico(s): Plant Pathogens and Fungal Diseases
1996 - Microbiology Society | Microbiology
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Nina Nauwelaerts, Neel Deferm, Anne Smits, Chiara Bernardini, Bart Lammens, Peggy Gandia, Alice Panchaud, Hedvig Nordeng, Maria Laura Bacci, Monica Forni, Domenico Ventrella, Kristel Van Calsteren, Anthony M. DeLise, Isabelle Huys, Michèle Bouisset-Leonard, Karel Allegaert, Pieter Annaert,
Breastfeeding plays a major role in the health and wellbeing of mother and infant. However, information on the safety of maternal medication during breastfeeding is lacking for most medications. This leads to discontinuation of either breastfeeding or maternal therapy, although many medications are likely to be safe. Since human lactation studies are costly and challenging, validated non-clinical methods would offer an attractive alternative. This review gives an extensive overview of the non-clinical ...
Tópico(s): Infant Nutrition and Health
2021 - Elsevier BV | Biomedicine & Pharmacotherapy
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Lien Dejager, Iris Pinheiro, Pieter Bogaert, Liesbeth Huys, Claude Libert,
ABSTRACT Infection with Salmonella enterica serovar Typhimurium is a complex disease in which the host-bacterium interactions are strongly influenced by genetic factors of the host. We demonstrate that SPRET/Ei, an inbred mouse strain derived from Mus spretus , is resistant to S. Typhimurium infections. The kinetics of bacterial proliferation, as well as histological examinations of tissue sections, suggest that SPRET/Ei mice can control bacterial multiplication and spreading despite significant attenuation ...
Tópico(s): interferon and immune responses
2010 - American Society for Microbiology | Infection and Immunity
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Jean‐Paul Pirnay, Gilbert Verbeken, Pieter‐Jan Ceyssens, Isabelle Huys, Daniël De Vos, Charlotte Ameloot, Alan Fauconnier,
Since time immemorial, phages—the viral parasites of bacteria—have been protecting Earth’s biosphere against bacterial overgrowth. Today, phages could help address the antibiotic resistance crisis that affects all of society. The greatest hurdle to the introduction of phage therapy in Western medicine is the lack of an appropriate legal and regulatory framework. Belgium is now implementing a pragmatic phage therapy framework that centers on the magistral preparation (compounding pharmacy in the US) ...
Tópico(s): Microbial infections and disease research
2018 - Multidisciplinary Digital Publishing Institute | Viruses
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Ivan Lule, Pieter-Jan D’Huys, Lieve Van Mellaert, Jozef Anné, Kristel Bernaerts, Jan Van Impe,
The metabolic impact exerted on a microorganism due to heterologous protein production is still poorly understood in Streptomyces lividans. In this present paper, based on exometabolomic data, a proposed genome-scale metabolic network model is used to assess this metabolic impact in S. lividans. Constraint-based modeling results obtained in this work revealed that the metabolic impact due to heterologous protein production is widely distributed in the genome of S. lividans, causing both slow substrate ...
Tópico(s): Enzyme Catalysis and Immobilization
2013 - Elsevier BV | Mathematical Biosciences
... dat alle dese schepen sonder ladinghe moestten t' huys keeren. Dit kasteel is soo gheleght, dat het ...
0000 - Gale Group | SASII
Ivan Lule, Bárbara Maldonado, Pieter-Jan D’Huys, Lieve Van Mellaert, Jan Van Impe, Kristel Bernaerts, Jozef Anné,
Tópico(s): Biochemical and Molecular Research
2012 - Springer Science+Business Media | Applied Microbiology and Biotechnology