C. M. Hardy, Carol Midgley and Alexandra Frean, Jon Ashworth, Tunku Varadarajan, Sam Kiley and Ben Macintyre, Richard Ford, Home Correspondent, Jasper Rees, Andrew Longmore, Alexandra Frean, Media Correspondent, Inigo Gilmore, Harvey Elliott, Richard Hobson, James Landale, Political Reporter, William Waldegrave, Jeremy Kingston, Joanna Bale, William Clarke, Binyamin Netanyahu, Lynne Truss, Carl Mortished, Nick Nuttall, Environment Correspondent, Morag Preston, Tom Walker, Geoff Brown, Gerald Davies, Clare Stewart, Robert Sheehan, Bridge Correspondent, Michael Latham, Arthur Leathley Political Correspondent, John Bryant, Polly Newton, Political Reporter, Bridget Harrison, Steve Keenan, David Charter Education Correspondent, Janet Bush, Economics Editor, Peter Ball, Raymond Keene Chess Correspondent, Audrey Magee Ireland Correspondent, Bernard Weatherill, Peter Ackroyd, Nick Nuttall Technology Correspondent, Peter Waymark, Alan Hamilton, Stephen Farrell, David Dabydeen, Robert Bruce, Pattrick Smellie, Quentin Letts, Robert D. Ballard, John Fletcher, Ian McIntyre, Peter Barnard, David Charter, King Hussein, Russell Kempson, Paul Durman, Julian Kossoff, Audrey Magee, Ireland Correspondent, David Miller, Richard Dunwoody, Frances Gibb and Jeremy Laurance, Arthur Hearnden General Secretary, Dalya Alberge, Arts Correspondent, Gillian Bowditch, Scotland Correspondent, Andrew Purkis, Adam Jones, Raymond Keene, Peter Davalle, Alan Lee, Cricket Correspondent, Emma Wilkins, John Grigg, Jonathan Prynn Transport Correspondent, P. H. S, Richard Evans, Bronwen Maddox, Harvey Elliott and Steve Keenan, Wade Mansell, Damian Whitworth, Susan Gray, Roger Boyes, Nigel Hawkes, Science Editor, Sarah Cunningham, Dominic Kennedy Social Affairs Correspondent, Andrew Pierce Political Correspondent, Philip Webster, Political Editor, Tony Bingham, Julian Muscat, Christopher Thomas, William Rees-Mogg, Eileen McCabe, Giles Whittell, Christopher Walker, Carol Midgley and Adrian Lee, Jill Sherman and Dominic Kennedy, Tim Hames, Mel Webb, Des Dearlove, Kathryn Knight, Matthew Parris, Richard Evans Racing Correspondent, Wendy Mullins, Anthony Loyd, Alan Challoner, Graham Searjeant, Jason Cowley, Nigel Hawkes Science Editor, Paul Cavadino, Tony Garland, Benedict Nightingale, Tess Knighton, Tom Rhodes, Dr Thomas Stuttaford, Rob Andrew, Stuart Etherington, Michael Henderson, Doug Counsell, Alasdair Murray, John Goodbody, Sports News Correspondent, Reginald Hibbert, Bryan Reuben, Orlando Figes, David Hands, Rugby Correspondent, Jonathan Mirsky, Christopher Irvine, Christine Buckley, Industrial Correspondent, M. Vandenberg,
... held up banks with her son's toy pisto. the drama had a tragic ending Admiral Dell ...
1997 - Gale Group | TDA
Paula Vainio, Santosh Gupta, Kirsi Ketola, Tuomas Mirtti, John-Patrick Mpindi, Pekka Kohonen, Vidal Fey, Merja Perälä, Frank Smit, Gerald W. Verhaegh, Jack A. Schalken, Kalle Alanen, Olli Kallioniemi, Kristiina Iljin,
... Ojala K. Iljin K. Bucher E. Sara H. Pisto T. Saarela M. Skotheim R.I. Björkman M. ... Ojala K. Iljin K. Bucher E. Sara H. Pisto T. Saarela M. Skotheim R.I. Björkman M. ...
Tópico(s): Prostate Cancer Treatment and Research
2011 - Elsevier BV | American Journal Of Pathology
... correlation with cardiovascular disease by multivariate analysis [4].Pisto et al. followed 988 persons from 1991–2009 [ ... 1579–1584 (2007).Crossref, Medline, CAS, Google Scholar5 Pisto P, Santaniemi M, Bloigu R et al. Fatty ...
Tópico(s): Liver Disease and Transplantation
2016 - Future Medicine | Future Cardiology
Constantinos Pistos, James T. Stewart,
A direct plasma injection HPLC method has been developed for the determination of selected benzodiazepines (nitrazepam, clobazam, oxazepam, lorazepam). The method uses an analytical hydrophobic shielded phase (Hisep) column equipped with a Hisep guard column, are easy to perform and requires 20 ul of a filtered plasma sample. The chromatographic run time is less than 15 min using a mobile phase of 15:85 v/v acetonitrile–0.18 M ammonium acetate pH 2.5. The method is good for 175 injections before replacement ...
Tópico(s): Molecular Sensors and Ion Detection
2003 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Constantinos Pistos, James T. Stewart,
A direct plasma injection HPLC method has been developed for the determination of selected phenothiazines (promethazine, promazine, chlorpromazine) using a Hisep column. The method is easy to perform and requires 20 microL of a filtered plasma sample. The chromatographic run time is less than 11 min using a mobile phase of 15:85 v/v acetonitrile-0.18 m ammonium acetate pH 5.0 and UV detection at 254 nm. The method is linear in the concentration range 0.1-25 microg mL(-1) (r > 0.99, n = 6) for each analyte ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2003 - Wiley | Biomedical Chromatography
Jonathan S. Jahr, Jeffrey D. Pisto, Melvin C. Gitlin, Patrick T. O Neill,
Sertraline is a new antidepressant categorized as a selective serotonin (5-hydroxytryptamine; 5-HT) reuptake inhibitor (SSRI) (1).This class of drugs reversibly blocks the reuptake of 5-HT in the synaptic cleft (1). Sertraline is efficacious in the treatment of depression, obsessive-compulsive, eating, and personality disorders (2). It is safe and well tolerated based upon worldwide experience (3). We report, however, a case of prolonged myoclonus in a patient who underwent a minor orthopedic procedure ...
Tópico(s): Parkinson's Disease and Spinal Disorders
1994 - Lippincott Williams & Wilkins | Anesthesia & Analgesia
Constantinos Pistos, Ioannis Papoutsis, Artemisia Dona, Maria Stefanidou, Sotiris Athanaselis, Constantinos Maravelias, Chara Spiliopoulou,
A simple HPLC method has been validated for the determination of sildenafil and its active metabolite (N-desmethylsildenafil) in human blood, using an octadecyl silica (ODS) hypersil column. The chromatographic run time is less than 25 min using a mobile phase of 35:65 (v/v) acetonitrile–0.015 M disodium hydrogen phosphate (Na2HPO4), triethylamine 0.1%, pH 7.4 at 1 mL/min flow rate and UV–vis detection at 230 nm. The method is linear in the concentration range of 10–500 ng/mL (r > 0.999, n = 5) for each ...
Tópico(s): Urinary Bladder and Prostate Research
2008 - Elsevier BV | Forensic Science International
Sami Kilpinen, Reija Autio, Kalle Ojala, Kristiina Iljin, Elmar Bucher, Henri Sara, Tommi Pisto, Matti Saarela, Rolf I. Skotheim, Mari Björkman, John‐Patrick Mpindi, Saija Haapa-Paananen, Paula Vainio, Henrik Edgren, Maija Wolf, Jaakko Astola, Matthias Nees, Sampsa Hautaniemi, Olli Kallioniemi,
Abstract Our knowledge on tissue- and disease-specific functions of human genes is rather limited and highly context-specific. Here, we have developed a method for the comparison of mRNA expression levels of most human genes across 9,783 Affymetrix gene expression array experiments representing 43 normal human tissue types, 68 cancer types, and 64 other diseases. This database of gene expression patterns in normal human tissues and pathological conditions covers 113 million datapoints and is available ...
Tópico(s): Cancer Genomics and Diagnostics
2008 - BioMed Central | Genome biology
Panagiota Nikolaou, Ioannis Papoutsis, Constantinos Maravelias, Chara Spiliopoulou, Constantinos Pistos, Antony C. Calokerinos, Julia Atta‐Politou,
Methadone is used extensively for the maintenance of opioid-addicted pregnant women. Because methadone and the two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyrroline (EMDP), are excreted into breast milk, a sensitive and specific gas chromatographic-mass spectrometric method has been developed, optimized, and validated for their quantitative determination in human breast milk. The procedure combined protein precipitation with acetonitrile ...
Tópico(s): Metabolism and Genetic Disorders
2008 - Oxford University Press | Journal of Analytical Toxicology
Ioannis Papoutsis, Sotiris Athanaselis, Panagiota Nikolaou, Constantinos Pistos, Chara Spiliopoulou, Constantinos Maravelias,
Benzodiazepines are used widely in daily clinical practice, due to their multiple pharmacological actions. The frequent problems associated with the wide use of benzodiazepines, as well as the multiple incidents of poisonings, led to the necessity for the development of a precise, sensitive and rapid method for the simultaneous determination of the 23 most commonly used benzodiazepines (diazepam, nordiazepam, oxazepam, bromazepam, alprazolam, lorazepam, medazepam, flurazepam, fludiazepam, tetrazepam, ...
Tópico(s): Poisoning and overdose treatments
2010 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Ioannis Papoutsis, Panagiota Nikolaou, Sotiris Athanaselis, Maria Stefanidou, Constantinos Pistos, Chara Spiliopoulou, Constantinos Maravelias,
Anticholinergic plants contain a variety of alkaloids that are toxic if ingested. Datura innoxia belongs to the family of Solanaceae and contains two main toxic alkaloids, atropine and scopolamine.In this study we report the case series of seven individuals who were admitted to two different hospitals of Athens with an anticholinergic syndrome. All symptoms manifested after consumption of cooked vegetables (blites).The investigation of the cases revealed that among the vegetables there was also Datura ...
Tópico(s): Drug-Induced Hepatotoxicity and Protection
2010 - Taylor & Francis | Clinical Toxicology
Ioanna Vardakou, Constantinos Pistos, Chara Spiliopoulou,
The present review highlights the existing monitoring and legislation status of synthetic cannabinoids in “Spice” products and alert research community about the identification and risk assessment problems of these compounds. Available data were collected by various literature search engines. All valuable information about psychoactive properties, safety profile, clinical data and detection problems for synthetic cannabinoids and their use as “herbal highs” were managed to spot and summarise. “Spice” ...
Tópico(s): Psychedelics and Drug Studies
2010 - Elsevier BV | Toxicology Letters
Ioanna Vardakou, Artemisia Dona, Constantinos Pistos, G. Alevisopoulos, Sotiris Athanaselis, Christos T. Maravelias, Chara Spiliopoulou,
A sensitive and specific GC/MS method for the determination of clozapine (CLZ) and its major metabolite norclozapine (NCLZ), in plasma has been developed, optimized and validated. Specimen preparation includes solid-phase extraction of both analytes using Bond-Elut Certify cartridge and further derivatization with TFAA. Clozapine-d8 was used as internal standard for the determination of CLZ and NCLZ. Limits of detection were 0.45 ng/mL for CLZ and 1.59 ng/mL for NCLZ, while limits of quantification ...
Tópico(s): Schizophrenia research and treatment
2010 - Elsevier BV | Journal of Chromatography B
Ioanna Vardakou, Constantinos Pistos, Chara Spiliopoulou,
Recently a new class of "designer drugs" has emerged on the drugs abuse market, known as "legal highs". Such drugs are legal to use and possess, and legal to supply. Mephedrone, a central nervous system stimulant, is the most widely experienced "legal high". This review presents any available information about psychoactive properties, safety profile, clinical data, and legislation of the new "legal high" and emphasizes the role of Internet with mephedrone's expansion. Available data were collected by ...
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2010 - Elsevier BV | Toxicology Letters
Kilian Guse, João D. Dias, Gerd Bauerschmitz, T Hakkarainen, Einari Aavik, Tuuli Ranki, Tommi Pisto, Merja Särkioja, R A Desmond, Anna Kanerva, Akseli Hemminki,
Tópico(s): Cancer Research and Treatments
2007 - Springer Nature | Gene Therapy
Alaa Khraiwesh, Ioannis Papoutsis, Panagiota Nikolaou, Constantinos Pistos, Chara Spiliopoulou, Sotiris Athanaselis,
A sensitive and specific GC/MS method for the determination of sertraline and its main metabolite desmethyl-sertraline in whole blood has been developed, optimized and validated. Sample preparation included solid-phase extraction of both analytes and their derivatization with heptafluorobutyric anhydride (HFBA). Protriptyline was used as internal standard for the determination of both analytes. Limits of detection and quantification for both sertraline and desmethyl-sertraline were 0.30 and 1.00 ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2011 - Elsevier BV | Journal of Chromatography B
Ioannis Papoutsis, Panagiota Nikolaou, Sotiris Athanaselis, Giorgos Alevizopoulos, Constantinos Pistos, Christina Paraskevopoulou, Chara Spiliopoulou,
Abstract Sildenafil (SDL) is a phosphodiesterase type 5 inhibitor and it is approved for the treatment of erectile dysfunction and pulmonary hypertension. SDL is extensively metabolized to its pharmacologically active metabolite, desmethyl‐sildenafil (DSDL). A sensitive and specific GC/MS method for the determination of SDL and DSDL in whole blood was developed and validated to support therapeutic drug monitoring of SDL patients. The combination of solid‐phase extraction with derivatization using ...
Tópico(s): Pharmaceutical Quality and Counterfeiting
2011 - Wiley | Journal of Separation Science
Panagiota Nikolaou, Ioannis Papoutsis, Sotiris Athanaselis, G. Alevisopoulos, Alaa Khraiwesh, Constantinos Pistos, Chara Spiliopoulou,
Tadalafil is a phosphodiesterase type 5 (PDE-5) inhibitor and it is used in the treatment of pulmonary arterial hypertension and erectile dysfunction. A sensitive and specific method is described for the determination of tadalafil in whole blood. Tadalafil and its internal standard (protriptyline) were isolated from the matrix by solid phase extraction, and were analyzed by gas chromatography/mass spectrometry (GC/MS) after derivatization by N,O-bis(trimethylsilyl)-trifluoracetamide (BSTFA) with 1% ...
Tópico(s): Phosphodiesterase function and regulation
2011 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
John Patrick Mpindi, Henri Sara, Saija Haapa-Paananen, Sami Kilpinen, Tommi Pisto, Elmar Bucher, Kalle Ojala, Kristiina Iljin, Paula Vainio, Mari Björkman, Santosh Gupta, Pekka Kohonen, Matthias Nees, Olli Kallioniemi,
Meta-analysis of gene expression microarray datasets presents significant challenges for statistical analysis. We developed and validated a new bioinformatic method for the identification of genes upregulated in subsets of samples of a given tumour type ('outlier genes'), a hallmark of potential oncogenes.A new statistical method (the gene tissue index, GTI) was developed by modifying and adapting algorithms originally developed for statistical problems in economics. We compared the potential of the ...
Tópico(s): Molecular Biology Techniques and Applications
2011 - Public Library of Science | PLoS ONE
Ioanna Vardakou, Constantinos Pistos, Artemisia Dona, Chara Spiliopoulou, Sotiris Athanaselis,
Ioanna Vardakou*a, Constantinos Pistosa, Artemis Donaa, Chara Spiliopouloua & Sotiris Athanaselisaa Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2011 - Taylor & Francis | Drug and Chemical Toxicology
Pauliina Pisto, Olavi Ukkola, Merja Santaniemi, Y. Antero Kesäniemi,
Proinflammatory cytokines and adipokines have a significant role in the development and progression of fatty liver. The aim of our current study was to explore the major indicators for hepatic fat determined as liver brightness. In addition to peptide hormones, several known cardiovascular and metabolic risk factors were included in the model. This is a population-based, epidemiological, cross-sectional study where 1200 subjects (600 men and 600 women, aged 40-59 years) were randomly selected, half ...
Tópico(s): Adipose Tissue and Metabolism
2011 - Elsevier BV | Metabolism
Ioannis Papoutsis, Alaa Khraiwesh, Panagiota Nikolaou, Constantinos Pistos, Chara Spiliopoulou, Sotiris Athanaselis,
Antidepressant drugs are widely used for the treatment of depression and other psychiatric disorders and as a result they are involved in numerous clinical and forensic cases. The aim of this study was the development, optimization and validation of a simple, specific and sensitive GC/MS method for the simultaneous determination of 11 antidepressant drugs and 4 of their metabolites (amitriptyline, citalopram, clomipramine, fluoxetine, fluvoxamine, maprotiline, desmethyl-maprotiline, mirtazapine, ...
Tópico(s): Forensic Toxicology and Drug Analysis
2012 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Sevasti Karampela, Ioanna Vardakou, Ioannis Papoutsis, Artemisia Dona, Chara Spiliopoulou, Sotiris Athanaselis, Constantinos Pistos,
A simple and rapid LC/MS method with direct injection analysis was developed and validated for the identification and quantification of ten benzodiazepines (flunitrazepam, nordiazepam, diazepam, 7-aminoflunitrazepam, flurazepam, bromazepam, midazolam, alprazolam, temazepam and oxazepam) in human urine using diazepam-d5 as internal standard (IS). The main advantage of the proposed methodology is the minimal sample preparation procedure, as diluted urine samples were directly injected into LC/MS system. ...
Tópico(s): Analytical Chemistry and Chromatography
2012 - Elsevier BV | Journal of Chromatography B
Ioannis Papoutsis, Marcela Mendonis, Panagiota Nikolaou, Sotiris Athanaselis, Constantinos Pistos, Constantinos Maravelias, Chara Spiliopoulou,
Abstract: Anticholinesterase pesticides are widely used, and as a result they are involved in numerous acute and even fatal poisonings. The aim of this study was the development, optimization, and validation of a simple, rapid, specific, and sensitive gas chromatography–mass spectrometry method for the determination of 11 anticholinesterase pesticides (aldicarb, azinphos methyl, carbofuran, chlorpyrifos, dialifos, diazinon, malathion, methamidophos, methidathion, methomyl, and terbufos) in blood. ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2012 - Wiley | Journal of Forensic Sciences
Constantinos Pistos, Maria Koutsopoulou, Irene Panderi,
Abstract An improved liquid chromatographic tandem mass spectrometric method for the determination of glimepiride in human plasma has been developed and fully validated. The article describes in detail the bioanalytical procedure and summarizes the validation results obtained. The samples were extracted using liquid–liquid extraction with a mixture of 1‐chlorobutane–isopropanol–ethyl acetate (88:2:10, v/v/v). The chromatographic separation was performed on a reversed‐phase Hypersil ODScolumn (250 × ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2005 - Wiley | Biomedical Chromatography
Constantinos Pistos, Maria Koutsopoulou, Irene Panderi,
A liquid chromatographic tandem mass spectrometric method for the determination of trandolapril in human plasma has been developed and fully validated. The article describes, in detail, the bioanalytical procedure and summarizes the validation results obtained. The samples were extracted using HLB Oasis solid-phase extraction cartridges. The chromatographic separation was performed on X-Terra C8 MS column (150 mm × 4.6 mm i.d., 5 μm particle size) using a mobile phase consisting of acetic acid 20 mM ...
Tópico(s): Pesticide Residue Analysis and Safety
2005 - Elsevier BV | Analytica Chimica Acta
Constantinos Pistos, Anna Tsantili‐Kakoulidou, Michael Α. Koupparis,
Tópico(s): Microfluidic and Capillary Electrophoresis Applications
2005 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Constantinos Pistos, James T. Stewart,
A fast and sensitive high performance liquid chromatography (HPLC) assay was developed on a C18 monolithic column for the simultaneous determination of acetaminophen–caffeine–butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated using a mobile phase of 95:5 (v/v) 0.1 M potassium phosphate monobasic (pH 2.41)–acetonitrile on the C18 monolithic column with detection at 220 nm. Benzoic acid was used as the internal standard (IS). The ...
Tópico(s): Pharmacological Effects and Assays
2004 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Constantinos Pistos, Irene Panderi, Julia Atta‐Politou,
A rapid, sensitive and novel narrow-bore liquid chromatography-mass spectrometric method was developed and fully validated for the quantification of citalopram in human plasma. The analyte and internal standard (imipramine) were extracted by liquid-liquid extraction with a mixture of hexane-heptane-isopropanol (88:10:2, v/v/v). The use of a Hypersil BDS C(8) micro-bore column (250 mm x 2.1 mm i.d.; 3.5 microm particle size), results in substantial reduction in solvent consumption. The mobile phase ...
Tópico(s): Analytical Methods in Pharmaceuticals
2004 - Elsevier BV | Journal of Chromatography B
Constantinos Pistos, Maria Koutsopoulou, Irene Panderi,
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2004 - Elsevier BV | Analytica Chimica Acta