Laurent Blairon, Roberto Cupaiolo, Isabelle Thomas, Sébastien Piteüs, Alain Wilmet, Ingrid Beukinga, Marie Tré‐Hardy,
Abstract More and more rapid antigen tests for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) appear in the market with varying performance. The sensitivity of these tests heavily depends on the viral load, extrapolated by the threshold cycle (Ct). It is therefore essential to verify their performance before their inclusion in routine. The Coronavirus Ag Rapid Test Cassette Bio‐Rad, the GSD NovaGen SARS‐CoV‐2 (COVID‐19) Antigen Rapid Test, and the Aegle Coronavirus Ag ...
Tópico(s): Biosensors and Analytical Detection
2021 - Wiley | Journal of Medical Virology
Laurent Blairon, Sébastien Piteüs, Ingrid Beukinga, Marie Tré‐Hardy,
Abstract The occurrence of the COVID‐19 second‐wave outbreak in Europe has pushed laboratories performing molecular SARS‐CoV‐2 tests to increase their throughput and decrease the result rendering time. In this evaluation, we tested for the first time a new, extraction‐free, protocol with the Allplex SARS‐CoV‐2 Assay RT‐qPCR kit on a Nimbus platform. Ninety‐one samples, of which 71 previously tested positive with RT‐qPCR with extraction were immediately analyzed without extraction, using only a dilution ...
Tópico(s): Biosensors and Analytical Detection
2021 - Wiley | Journal of Medical Virology
Laurent Blairon, Roberto Cupaiolo, Sébastien Piteüs, Ingrid Beukinga, Marie Tré‐Hardy,
Following the emergence of SARS-CoV-2 variants of concern (VOCs) worldwide, it is important to monitor local epidemiology to better understand the occurrence of clusters, reinfections, or infection after vaccination. Detecting mutations by specific RT-qPCR is a rapid and affordable alternative to sequencing. However, care must be taken to ensure that the techniques used are up-to-date and adapted to the variants circulating in the studied population.All samples tested positive for SARS-CoV-2 were ...
Tópico(s): Viral gastroenteritis research and epidemiology
2021 - Elsevier BV | Journal of Virological Methods
María Luisa Cerdeño Serrano, Olga De Miguel,
... Materiales de importación en la necrópolis de Can Piteu-Can Roqueta (Sabadell, Barcelona)”, en S. Celestino y ...
Tópico(s): Medieval Architecture and Archaeology
2022 - University of Castile-La Mancha | Vínculos de Historia Revista del Departamento de Historia de la Universidad de Castilla-La Mancha
Marie Tré‐Hardy, Sébastien Piteüs, Ingrid Beukinga, Laurent Blairon,
Faced with the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-throughput respiratory tests are in high demand. We evaluated the clinical performance of the GSD NovaPrime® SARS-CoV-2 RTq-PCR assay, a new assay that detects 2 specific RNA sequences of the nucleocapsid (N) gene. It was assessed using 99 nasopharyngeal samples and compared in parallel with the Allplex® assay. Among those samples, 72 and 27 were included in the positive (PPA) and negative (NPA) percent agreement ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2022 - Elsevier BV | Diagnostic Microbiology and Infectious Disease