Harvie P. Portugaliza, Shinya Miyazaki, Fiona J. A. Geurten, Christopher Pell, Anna Rosanas‐Urgell, Chris J. Janse, Alfred Cortés,
Malaria transmission is dependent on the formation of gametocytes in the human blood. The sexual conversion rate, the proportion of asexual parasites that convert into gametocytes at each multiplication cycle, is variable and reflects the relative parasite investment between transmission and maintaining the infection. The impact of environmental factors such as drugs on sexual conversion rates is not well understood. We developed a robust assay using gametocyte-reporter parasite lines to accurately ...
Tópico(s): Complement system in diseases
2020 - eLife Sciences Publications Ltd | eLife
Harvie P. Portugaliza, Oriol Llorà-Batlle, Anna Rosanas‐Urgell, Alfred Cortés,
Abstract Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact ...
Tópico(s): Complement system in diseases
2019 - Nature Portfolio | Scientific Reports
Harvie P. Portugaliza, Beatriz Galatas, Hoticha Nhantumbo, Helder Djive, Ilda Murato, Francisco Saúte, Pedro Aíde, Christopher Pell, Khátia Munguambe,
In a background of renewed calls for malaria eradication, several endemic countries in sub-Saharan Africa are contemplating malaria elimination nationally or sub-nationally. In Mozambique, a strategy to eliminate malaria in the south is underway in the context of low endemicity levels and cross-border initiatives to eliminate malaria in South Africa and Eswatini. In this context, a demonstration project aiming to interrupt malaria transmission through mass antimalarial drug administrations and intensified ...
Tópico(s): Parasites and Host Interactions
2019 - BioMed Central | Malaria Journal
Delfin E. Cabardo, Harvie P. Portugaliza,
Haemonchus contortus is one of the prevalent and pathogenic ruminant parasites that has grown resistance to common anthelmintic treatment. This study evaluated the anthelmintic potential of Moringa oleifera seed ethanolic and aqueous extracts against H. contortus eggs and infective stage larvae (L3s). The efficacy of five extract concentrations (0.95, 1.95, 3.9, 7.8, and 15.6 mg/mL) were tested through egg hatch assay and larval motility test. Phytochemical tests were conducted to detect the different ...
Tópico(s): Rabbits: Nutrition, Reproduction, Health
2017 - Taylor & Francis | International Journal of Veterinary Science and Medicine
Ariel Paul M. Rupa, Harvie P. Portugaliza,
Aim: Gastrointestinal parasitism is a serious constraint affecting goat production in the Philippines.This study aimed to determine the prevalence and associated risk factors of gastrointestinal nematode infection in goat-populated barangays of Baybay City, Leyte. Materials and Methods:A total of 81 households or farms were interviewed, and 450 goats were sampled for fecalysis.Fecal egg count along with egg morphological identification and coproculture for third stage larvae identification were ...
Tópico(s): Parasites and Host Interactions
2016 - Veterinary World | Veterinary World
Harvie P. Portugaliza, Teresa Fernández,
A study was conducted to determine the growth performance of Cobb broilers supplemented with varying concentrations of Moringa oleifera Aqueous Leaf Extract (MoALE) via the drinking water. A total of four hundred day-old chicks were randomly distributed into four treatment groups, replicated four times with twenty-five broilers per replicate. The growth performance of broilers was evaluated based on their feed consumption, live weight, feed conversion ratio (FCR) and return of investment (ROI). Results ...
Tópico(s): Animal Nutrition and Physiology
2012 - | Online Journal of Animal and Feed Research
Harvie P. Portugaliza, Ivy Mae C. Balaso, June Clyde B. Descallar, Eugene B. Lañada,
Fasciolosis remains an important parasitic disease of ruminants in Southeast Asia. We investigated the prevalence and spatial distribution of Fasciola spp. in carabao (Bubalus bubalis) and snail intermediate hosts (IHs) in Baybay, Leyte, Philippines. Using one-stage cluster sampling, we examined 335 fecal samples of carabaos from 138 herds for Fasciola spp. eggs, and in parallel collected IH snail data in the area. Risk factor analyses were performed and GIS maps were created for Fasciola spp. prevalence ...
Tópico(s): Coccidia and coccidiosis research
2018 - Elsevier BV | Veterinary Parasitology Regional Studies and Reports
Harvie P. Portugaliza, Melvin Ayes Bagot,
Tópico(s): Rabies epidemiology and control
2015 - CIPAV Foundation | Livestock research for rural development
Harvie P. Portugaliza, Hamtandi Magloire Natama, Pieter Guetens, Eduard Rovira-Vallbona, Anyirékun Fabrice Somé, Aida Millogo, Delwendé Florence Ouedraogo, Innocent Valéa, Hermann Sorgho, Halidou Tinto, Nguyen Van Hong, António Sitoe, Rosauro Varo, Quique Bassat, Alfred Cortés, Anna Rosanas‐Urgell,
Artemisinins (ART) are the key component of the frontline antimalarial treatment, but their impact on Plasmodium falciparum sexual conversion rates in natural malaria infections remains unknown. This is an important knowledge gap because sexual conversion rates determine the relative parasite investment between maintaining infection in the same human host and transmission to mosquitoes.The primary outcome of this study was to assess the impact of ART-based treatment on sexual conversion rates by ...
Tópico(s): Trypanosoma species research and implications
2022 - Elsevier BV | EBioMedicine
Núria Casas-Vila, Anastasia K. Pickford, Harvie P. Portugaliza, Elisabet Tintó-Font, Alfred Cortés,
In Plasmodium falciparum, the parasite responsible for the most severe forms of human malaria, many fundamental processes are controlled at the transcriptional level. Studies on diverse aspects of basic parasite biology as well as molecular epidemiology studies often rely on the ability to accurately measure transcript levels, but this is complicated by the cyclic expression patterns of the majority of malaria parasite genes. Here, we provide a complete workflow to measure transcript levels in P. ...
Tópico(s): Invertebrate Immune Response Mechanisms
2021 - Springer Science+Business Media | Methods in molecular biology
Marlon Dave P. Conde, Harvie P. Portugaliza, Eugene B. Lañada,
Toxocara canis is a parasitic gastrointestinal nematode of dogs causing toxocariasis in humans, a neglected tropical disease. This study examined the burden of T. canis infection in dogs and egg contamination in school playgrounds and public parks in Baybay City, Leyte, Philippines. Fecalysis and egg detection in soils were performed to determine the parasite prevalence in dogs and the environment. In addition, pet owner's survey and GIS mapping were employed to identify the risk factors of infection ...
Tópico(s): Pediatric health and respiratory diseases
2022 - Springer Nature | Journal of Parasitic Diseases
Harvie P. Portugaliza, Shiela Navarroza Romero, Melvin Ayes Bagot,
This case report describes the necropsy findings of two potentially zoonotic parasites infecting the Philippine brown deer (Cervus mariannus) in Leyte Island, Philippines. A female deer aging approximately 5-year was presented for necropsy to the Diagnostic Laboratory at the College of Veterinary Medicine, Visayas State University. Gross pathology was recorded and the selected organs having lesion were collected for histopathological studies. Results showed severe necrotizing lesions in the nasal ...
Tópico(s): Zoonotic diseases and public health
2015 - Network for the Veterinarians of Bangladesh | Journal of Advanced Veterinary and Animal Research
Objective: This study aims to determine the seroprevalence of African swine fever (ASF) in pigs for slaughter in Leyte, Philippines. It underpins the concept that recovered and infected pigs from ASF are likely sent for slaughter to avoid perceived economic losses. Materials and Methods: A cross-sectional study was conducted from March to June 2023 in eight abattoirs, each representing both a city and a municipality in Leyte province. A total of 218 blood samples from 78 farms were examined for ASF ...
Tópico(s): Animal Virus Infections Studies
2024 - Network for the Veterinarians of Bangladesh | Journal of Advanced Veterinary and Animal Research
Harvie P. Portugaliza, Irvin L. Tocmo, Tomas J. Fernandez,
The periodicity of parasite egg excretion refers to variations in the number of eggs produced across time, with significant implications in optimizing diagnostic procedures and conducting the Fecal Egg Count Reduction Test (FECRT). Here, we explore whether Ascaridia galli egg excretion varies across time under Philippine conditions, thus informing the best time to collect fecal samples during flock health examination. A time-course analysis was performed in chickens (N = 12) experimentally infected ...
Tópico(s): Parasite Biology and Host Interactions
2024 - Springer Nature | Journal of Parasitic Diseases