Produção Nacional
Revisado por pares
Marie Plourde, Jean-Michel Ubeda, Goutam Mandal, Rubens Lima do Monte‐Neto, Rita Mukhopadhyay, Marc Ouellette,
The Leishmania aquaglyceroporin AQP1 plays an important physiological role in water and uncharged polar solutes transport, volume regulation, osmotaxis, and is a key determinant of antimony resistance. By targeted gene disruption, we generated a Leishmania major promastigote AQP1 null mutant. This required several attempts but a chromosomal null AQP1 mutant was obtained by loss of heterozygosity in the presence of a rescue plasmid encoding AQP1. Growth in the absence of selection led to the loss ...
Tópico(s): Trypanosoma species research and implications
2015 - Elsevier BV | Molecular and Biochemical Parasitology
Produção Nacional
Revisado por pares
Joana Darc S. Chaves, Luiza Guimarães Tunes, Chris H. J. Franco, Thiago M. Francisco, Charlane C. Corrêa, Silvane Maria Fonseca Murta, Rubens Lima do Monte‐Neto, Heveline Silva, Ana Paula Soares Fontes, Mauro V. de Almeida,
The current anticancer and antileishmanial drug arsenal presents several limitations concerning their specificity, efficacy, costs and the emergence of drug-resistant cells lines, which encourages the urgent need to search for new alternatives. Inspired by the fact that gold(I)-based compounds are promising antitumoral and antileishmanial drug candidates, we synthesized novel gold(I) complexes containing phosphine and 5-phenyl-1,3,4-oxadiazole-2-thione and evaluated their anticancer and antileishmanial ...
Tópico(s): Ferrocene Chemistry and Applications
2016 - Elsevier BV | European Journal of Medicinal Chemistry
Produção Nacional
Revisado por pares
Daniel Abud Seabra Matos, José Rubens Lima Jardilino,
Nas pesquisas sobre “o que pensam” alunos, professores e atores educacionais, há uma enorme variedade de termos que se utiliza para referir-se às representações mentais. Essa diversidade acaba dificultando a compreensão de pesquisas da área educacional e seu arcabouço teórico- -conceitual. Há muitos estudos na literatura educacional que não definem claramente os conceitos utilizados. O objetivo deste artigo é realizar uma análise sobre os conceitos de concepção, percepção, representação e crença. ...
Tópico(s): Education Pedagogy and Practices
2016 - UNIVERSIDADE ESTADUAL DO CEARÁ | Educação & Formação
Produção Nacional
Revisado por pares
Raul Rio Ribeiro, Weverson Alves Ferreira, Patricia Silveira Martins, Rubens Lima do Monte‐Neto, Olguita G. Ferreira Rocha, Laurence Le Moyec, Cynthia Demicheli, Frédéric Frézard,
Abstract The orally active composition comprising meglumine antimoniate (MA) and β ‐cyclodextrin ( β ‐CD) differs markedly from conventional drug–CD complexes, since it combines a water‐soluble drug and a hydrophilic CD. In order to obtain insights into the mechanism(s) responsible for the improved oral delivery of the drug, physicochemical and pharmacokinetic studies were carried out. The composition investigated here was prepared at a 7:1 antimony(Sb)/ β ‐CD molar ratio, a condition that improves its ...
Tópico(s): Aluminum toxicity and tolerance in plants and animals
2009 - Wiley | Biopharmaceutics & Drug Disposition
Produção Nacional
Revisado por pares
Rubens Lima do Monte‐Neto, Marco Antonio Paula de Sousa, Celidarque S. Dias, José Maria Barbosa‐Filho, Márcia Rosa de Oliveira,
The in vitro cytotoxic potential of yangambin was evaluated. Yangambin is a pharmacologically active furofuran lignan obtained from the leaves of Ocotea duckei. It is the major compound from the lignoids fraction. Yangambin presented low cytotoxicity in all in vitro models analyzed. Its cytotoxicity to murine macrophages was measured by the Trypan blue dye exclusion test and MTT reduction assay, resulting in high CC 50 values of 187.0 μg/mL (383.3 μm) and 246.7 μg/mL (504.3 μm), respectively. The ...
Tópico(s): Synthetic Organic Chemistry Methods
2008 - De Gruyter | Zeitschrift für Naturforschung C
Tópico(s): Sociology and Education in Brazil
2008 - Pedagogical and Technological University of Colombia | Revista Historia de la Educación Latinoamericana
Produção Nacional
Revisado por pares
Rubens Lima do Monte‐Neto, Louisa M. A. Sousa, Celidarque S. Dias, José Maria Barbosa‐Filho, Márcia Rosa de Oliveira, Regina Célia Bressan Queiroz de Figueiredo,
We have previously demonstrated that yangambin, a lignan obtained from Ocotea duckei Vattimo (Lauraceae), shows antileishmanial activity against promastigote forms of Leishmania chagasi and Leishmania amazonensis. The aim of this study was to determine the in vitro effects of yangambin against these parasites using electron and confocal microscopy. L. chagasi and L. amazonensis promastigotes were incubated respectively with 50 μg/mL and 65 μg/mL of pure yangambin and stained with acridine orange. ...
Tópico(s): Research on Leishmaniasis Studies
2010 - Elsevier BV | Experimental Parasitology
Produção Nacional
Revisado por pares
Rubens Lima do Monte‐Neto, José Maria Barbosa‐Filho, Louisa M. A. Sousa, Petrônio F. Athayde Filho, Celidarque S. Dias, Márcia Rosa de Oliveira,
Crude ethanolic extract, lignoid fraction and the purified compound yangambin were obtained from Ocotea duckei (Lauraceae) and their antileishmanial activity was tested against promastigote forms of Leishmania chagasi and Leishmania amazonensis cultivated in Schneider medium, supplemented with 20% of fetal bovine serum. All substances presented antileishmanial activity with IC 50 values of 135.7 μg/mL for the crude ethanolic extract, 26.5 μg/mL for the lignoid fraction and 49.0 μg/mL for yangambin ...
Tópico(s): Phytochemical compounds biological activities
2007 - De Gruyter | Zeitschrift für Naturforschung C
Produção Nacional
Revisado por pares
Rubens Lima do Monte‐Neto, Adriano C. Coelho, Frédéric Raymond, Danielle Légaré, Jacques Corbeil, Maria Norma Melo, Frédéric Frézard, Marc Ouellette,
Background Drug resistance is a major problem in leishmaniasis chemotherapy. RNA expression profiling using DNA microarrays is a suitable approach to study simultaneous events leading to a drug-resistance phenotype. Genomic analysis has been performed primarily with Old World Leishmania species and here we investigate molecular alterations in antimony resistance in the New World species L. amazonensis. Methods/Principal Findings We selected populations of L. amazonensis promastigotes for resistance ...
Tópico(s): Trypanosoma species research and implications
2011 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
E. Jaimes, Rubens Lima do Monte‐Neto, Priscila G. Reis, Nelson G. Fernandes, Nivaldo L. Speziali, Maria do Carmo Barros de Melo, Frédéric Frézard, Cynthia Demicheli,
Two novel trivalent antimony(III) and bismuth(III) complexes with the nitrogen-donor heterocyclic ligand dipyrido[3,2-a:2',3'-c]phenazine (dppz) were synthesized and characterized as [Sb(dppz)Cl3]∙H2O∙CH3OH and [Bi(dppz)Cl3]. The crystal structure of Sb(III) complex was determined by X-ray crystallography. These complexes were evaluated for their activity against the promastigote form of Sb(III)-sensitive and –resistant Leishmania infantum chagasi and Leishmania amazonensis strains. Both complexes were ...
Tópico(s): Synthesis and Biological Evaluation
2012 - Multidisciplinary Digital Publishing Institute | Molecules
Philippe Leprohon, Christopher Fernandez‐Prada, Élodie Gazanion, Rubens Lima do Monte‐Neto, Marc Ouellette,
The use of next generation sequencing has the power to expedite the identification of drug resistance determinants and biomarkers and was applied successfully to drug resistance studies in Leishmania. This allowed the identification of modulation in gene expression, gene dosage alterations, changes in chromosome copy numbers and single nucleotide polymorphisms that correlated with resistance in Leishmania strains derived from the laboratory and from the field. An impressive heterogeneity at the ...
Tópico(s): Insect symbiosis and bacterial influences
2014 - Elsevier BV | International Journal for Parasitology Drugs and Drug Resistance
Produção Nacional
Revisado por pares
Frédéric Frézard, Rubens Lima do Monte‐Neto, Priscila G. Reis,
Antimonial compounds have been used for more than a century in the treatment of the parasitic disease leishmaniasis. Although pentavalent antimonials are still first-line drugs in several developing countries, this class of drugs is no longer recommended in the Indian sub-continent because of the emergence of drug resistance. The precise mechanisms involved in the resistance of leishmania parasites to antimony are still subject to debate. It is now well documented that drug resistance in leishmania ...
Tópico(s): Garlic and Onion Studies
2014 - Springer International Publishing | Biophysical Reviews
Produção Nacional
Revisado por pares
E. Jaimes, Priscila G. Reis, Filipe M. B. V. Bezerra, Bernardo L. Rodrigues, Rubens Lima do Monte‐Neto, Maria Norma Melo, Frédéric Frézard, Cynthia Demicheli,
Novel trivalent antimony complexes with the nitrogen donor heterocyclic ligand 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or dipyrido[3,2-d:2',3'-f]quinoxaline (dpq) have been synthesized by the reaction with SbCl3 or PhSbCl2. The crystal structures of [Sb(phen)Cl3] and [PhSb(phen)Cl2]CH3COOH were determined and shown to adopt a distorted square pyramid geometry with a five-coordinated Sb center. Surprisingly, all the complexes, the ligands and PhSbCl2 showed very high antileishmanial activities, ...
Tópico(s): Synthesis and Biological Evaluation
2013 - Elsevier BV | Journal of Inorganic Biochemistry
Produção Nacional
Revisado por pares
Tiago T. Guimarães, Maria do Carmo F. R. Pinto, Juliane S. Lanza, Maria Norma Melo, Rubens Lima do Monte‐Neto, Isadora M.M. de Melo, Emilay B. T. Diogo, Vı́tor F. Ferreira, Celso A. Câmara, Wagner O. Valença, Ronaldo N. de Oliveira, Frédéric Frézard, Eufrânio N. da Silva Júnior,
Continuing our screening program for novel anti-parasite compounds, we synthesized seven 1,4-naphthoquinones coupled to 1,2,3-triazoles, five nor-β-lapachone-based 1,2,3-triazoles and ten α-lapachone-based 1,2,3-triazoles. These and other naphthoquinonoid compounds were evaluated for their activity against promastigote forms of antimony-sensitive and -resistant strains of Leishmania infantum (syn. Leishmania chagasi) and Leishmania amazonensis. The toxicity of these compounds to mammalian cells was ...
Tópico(s): Synthesis and Biological Evaluation
2013 - Elsevier BV | European Journal of Medicinal Chemistry
Angana Mukherjee, Sébastien Boisvert, Rubens Lima do Monte‐Neto, Adriano C. Coelho, Frédéric Raymond, Rita Mukhopadhyay, Jacques Corbeil, Marc Ouellette,
Summary Antimonials are still the mainstay of treatment against leishmaniasis but drug resistance is increasing. We carried out short read next‐generation sequencing ( NGS ) and comparative genomic hybridization ( CGH ) of three independent L eishmania major antimony‐resistant mutants. Copy number variations were consistently detected with both NGS and CGH . A major attribute of antimony resistance was a novel terminal deletion of variable length (67 kb to 204 kb) of the polyploid chromosome 31 in the three ...
Tópico(s): Parasites and Host Interactions
2013 - Wiley | Molecular Microbiology
Produção Nacional
Revisado por pares
Douglas S. Moreira, Rubens Lima do Monte‐Neto, Juvana Moreira Andrade, Ana Maria Murta Santi, Priscila G. Reis, Frédéric Frézard, Silvane Maria Fonseca Murta,
ATP-binding cassette (ABC) transporters have been associated with drug resistance in various diseases. The MRPA gene, a transporter of ABCC subfamily, is involved in the resistance by sequestering metal-thiol conjugates in intracellular vesicles of Leishmania parasite. In this study, we performed the molecular characterization of the MRPA transporter, analysis of P-glycoprotein (Pgp) and aquaglyceroporin-1 (AQP1) expression, and determination of antimony level in antimony-susceptible and -resistant ...
Tópico(s): Parasites and Host Interactions
2013 - Elsevier BV | International Journal for Parasitology Drugs and Drug Resistance
Produção Nacional
Revisado por pares
Rubens Lima do Monte‐Neto, Marie-Claude N. Laffitte, Philippe Leprohon, Priscila G. Reis, Frédéric Frézard, Marc Ouellette,
Antimony resistance complicates the treatment of infections caused by the parasite Leishmania.Using next generation sequencing, we sequenced the genome of four independent Leishmania guyanensis antimony-resistant (SbR) mutants and found different chromosomal alterations including aneuploidy, intrachromosomal gene amplification and gene deletion. A segment covering 30 genes on chromosome 19 was amplified intrachromosomally in three of the four mutants. The gene coding for the multidrug resistance ...
Tópico(s): Trypanosoma species research and implications
2015 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Carolina Andion, Luciana Francisco de Abreu Ronconi, Rubens Lima Moraes, Aghata Karoliny Ribeiro Gonsalves, Lilian Brum Duarte Serafim,
Este artigo propõe uma nova leitura teórico-analítica para o estudo dos processos de inovação social promovidos por atores da sociedade civil na esfera pública, com base na sociologia pragmática francesa. Parte de uma discussão sobre o lugar das iniciativas da sociedade civil na ação pública na atualidade. Em seguida, faz uma incursão no debate sobre a incidência da sociedade civil na esfera pública, buscando evidenciar em que a sociologia pragmática francesa pode auxiliar para uma nova compreensão ...
Tópico(s): Education, sociology, and vocational training
2017 - FUNDAÇÃO GETÚLIO VARGAS | Revista de Administração Pública
Produção Nacional
Revisado por pares
Carolina Andion, Luciana Francisco de Abreu Ronconi, Rubens Lima Moraes, Aghata Karoliny Ribeiro Gonsalves, Lilian Brum Duarte Serafim,
Tópico(s): Innovation, Technology, and Society
2017 - FUNDAÇÃO GETÚLIO VARGAS | Revista de Administração Pública
Produção Nacional
Revisado por pares
Rubens Lima Moraes, Carolina Andion,
Tópico(s): Social Media and Politics
2017 - Springer Science+Business Media | VOLUNTAS International Journal of Voluntary and Nonprofit Organizations
Produção Nacional
Revisado por pares
Jerônimo Nunes Rugani, Patrícia Flávia Quaresma, Célia Maria Ferreira Gontijo, Rodrigo Pedro Soares, Rubens Lima do Monte‐Neto,
Leishmania (Viannia) braziliensis is the most common etiological agent of cutaneous and mucocutaneous leishmaniasis (MCL) in Latin America. An interesting aspect of the disease outcome caused by this species is the appearance of non-ulcerated atypical cutaneous leishmaniasis. Atypical (AT) lesions are often associated with therapeutic failure when treated with antimony(Sb)-based drugs. Refractory cases are not necessarily due to intrinsic parasite drug resistance. The status of in vitro drug susceptibility ...
Tópico(s): Parasites and Host Interactions
2018 - Elsevier BV | Biomedicine & Pharmacotherapy
Produção Nacional
Revisado por pares
Ana Maria Murta Santi, Juliane S. Lanza, Luiza Guimarães Tunes, Jacqueline Araújo Fiuza, Gaétan Roy, Alessandra da Silva Orfanó, Andréa Teixeira−Carvalho, Frédéric Frézard, André Luís Branco de Barros, Silvane Maria Fonseca Murta, Rubens Lima do Monte‐Neto,
There is no safe and efficacious vaccine against human leishmaniasis available and live attenuated vaccines have been used as a prophylactic alternative against the disease. In order to obtain an attenuated Leishmania parasite for vaccine purposes, we generated L. infantum KHARON1 (KH1) null mutants (ΔLikh1). This gene was previously associated with growth defects in L. mexicana. ΔLikh1 was obtained and confirmed by PCR, qPCR and Southern blot. We also generate a KH1 complemented line with the introduction ...
Tópico(s): Eosinophilic Disorders and Syndromes
2018 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
José Rubens Lima Jardilino, Ana Maria Mendes Sampaio,
O presente artigo tem como objetivo discutir sobre o Desenvolvimento Profissional Docente (DPD) frente à política pública do Plano Nacional de Educação (Lei13.005/2014). O texto examina conceitos, construídos porpesquisadores ibéricos (Garcia, 1999, 2009; Imbernón, 2004; Nóvoa, 1991, 2002, 2008) e nacionais (Almeida, 2000; André, 1999,2017; Gatti, 2017; Jardilino,2016; Oliveira, 2012; Ponte, 1998) sobre o DPD e empreende esforços analíticos em torno das metas do PNE (2014-2024), que dialogam com o desenvolvimento ...
Tópico(s): Education and Public Policy
2019 - UNIVERSIDADE ESTADUAL DO CEARÁ | Educação & Formação
Produção Nacional
Revisado por pares
Jerônimo Nunes Rugani, Célia Maria Ferreira Gontijo, Frédéric Frézard, Rodrigo Pedro Soares, Rubens Lima do Monte‐Neto,
BACKGROUND In addition to the limited therapeutic arsenal and the side effects of antileishmanial agents, drug resistance hinders disease control. In Brazil, Leishmania braziliensis causes atypical (AT) tegumentary leishmaniasis lesions, frequently refractory to treatment. OBJECTIVES The main goal of this study was to characterise antimony (Sb)-resistant (SbR) L. braziliensis strains obtained from patients living in Xakriabá indigenous community, Minas Gerais, Brazil. METHODS The aquaglyceroporin 1- ...
Tópico(s): Research on Leishmaniasis Studies
2019 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Andrés Villaseñor Espinosa, Danilo de Souza Costa, Luiza Guimarães Tunes, Rubens Lima do Monte‐Neto, Richard Michael Grazul, Mauro V. de Almeida, Heveline Silva,
Four gold(I) complexes conceived as anticancer agents were synthesized by reacting [Au(PEt3 )Cl] and [Au(PPh3 )Cl] with ligands derived from δ-d-gluconolactone. The ligands' structure was designed to combine desired biological properties previously reported for each group. Ligands were synthesized from δ-d-gluconolactone via ketal protection and hydrazide formation followed by cyclization with CS2 to produce the novel oxadiazolidine-2-thione 7 and 8. Increasing of the ligands' lipophilicity via ketal ...
Tópico(s): Metal complexes synthesis and properties
2020 - Wiley | Chemical Biology & Drug Design
Produção Nacional
Luiza Guimarães Tunes, Roberta E. Morato, Adriana García, Vinicius Schmitz, Mário Steindel, José Dias Corrêa, Hélio F. Dos Santos, Frédéric Frézard, Mauro V. de Almeida, Heveline Silva, Nilmar Silvio Moretti, André Luís Branco de Barros, Rubens Lima do Monte‐Neto,
The drugs currently used to treat leishmaniases have limitations concerning cost, efficacy, and safety, making the search for new therapeutic approaches urgent. We found that the gold(I)-derived complexes were active against L. infantum and L. braziliensis intracellular amastigotes with IC50 values ranging from 0.5 to 5.5 μM. All gold(I) complexes were potent inhibitors of trypanothione reductase (TR), with enzyme IC50 values ranging from 1 to 7.8 μM. Triethylphosphine-derived complexes enhanced ...
Tópico(s): Parasites and Host Interactions
2020 - American Chemical Society | ACS Infectious Diseases
Produção Nacional
Revisado por pares
Arijit Bhattacharya, Audrey Corbeil, Rubens Lima do Monte‐Neto, Christopher Fernandez‐Prada,
Leishmaniasis (Leishmania species), sleeping sickness (Trypanosoma brucei), and Chagas disease (Trypanosoma cruzi) are devastating and globally spread diseases caused by trypanosomatid parasites. At present, drugs for treating trypanosomatid diseases are far from ideal due to host toxicity, elevated cost, limited access, and increasing rates of drug resistance. Technological advances in parasitology, chemistry, and genomics have unlocked new possibilities for novel drug concepts and compound screening ...
Tópico(s): Synthesis and Biological Evaluation
2020 - Multidisciplinary Digital Publishing Institute | Genes
Izhar Wallach, Denzil Bernard, Kong T. Nguyen, Gregory Ho, Adrian R. Morrison, Adrian Stecuła, Andreana M. Rosnik, Ann O’Sullivan, Aram Davtyan, Ben Samudio, Bill Thomas, Brad Worley, Brittany Butler, Christian Laggner, Desiree A. Thayer, Ehsan Moharreri, Greg Friedland, Ha H. Truong, Henry van den Bedem, Ho Leung Ng, Kate A. Stafford, Krishna K. Sarangapani, Kyle Giesler, Lien Ngo, Michael M. Mysinger, Mostafa Ahmed, Nicholas J. Anthis, Niel M. Henriksen, Paweł Gniewek, S.R. Eckert, Saulo de Oliveira, Shabbir Suterwala, Srimukh Veccham Krishna PrasadPrasad, Stefani Shek, Stephanie Contreras, Stephanie R. Hare, Teresa A. Palazzo, Terrence E. O’Brien, Tessa Van Grack, Tiffany Williams, Ting‐Rong Chern, Victor Kenyon, Andreia H. Lee, Andrew B. Cann, Bastiaan Bergman, Brandon Anderson, Bryan D. Cox, Jeffrey M. Warrington, Jon M. Sorenson, Joshua M. Goldenberg, Matthew A. Young, Nicholas DeHaan, Ryan P. Pemberton, Stefan Schroedl, Tigran M. Abramyan, T. Raghavendra Gupta, Venkatesh Mysore, Adam G. Presser, Adolfo A. Ferrando, Adriano D. Andricopulo, Agnidipta Ghosh, Aicha Gharbi Ayachi, Aisha Mushtaq, Ala M. Shaqra, Alan Kie Leong Toh, Alan V. Smrcka, Alberto Ciccia, Aldo Sena de Oliveira, Aleksandr Sverzhinsky, Alessandra Mara de Sousa, Alexander I. Agoulnik, Alexander Kushnir, Alexander N. Freiberg, Alexander V. Statsyuk, Alexandre R. Gingras, Alexei Degterev, Alexey Tomilov, Alice Vrielink, Alisa A. Garaeva, Amanda Bryant-Friedrich, Amedeo Caflisch, Amit K. Patel, Amith Vikram Rangarajan, An Matheeussen, Andrea Battistoni, Andrea Caporali, Andrea Chini, Andrea Ilari, Andrea Mattevi, Andrea Foote, Andrea Trabocchi, Andreas Stahl, Andrew B. Herr, Andrew D. Berti, Andrew Freywald, Andrew G. Reidenbach, Andrew Lam, Andrew Cuddihy, Andrew D. White, Angelo Taglialatela, Anil K. Ojha, Ann M. Cathcart, Anna A. L. Motyl, Anna Borowska, Anna D’Antuono, Anna K. H. Hirsch, Anna Maria Porcelli, Anna Minakova, Anna Montanaro, Anna Müller, Annarita Fiorillo, Anniina Virtanen, Anthony J. O’Donoghue, Antonio Del Rio Flores, Antonio E. Garmendia, Antonio Pineda‐Lucena, Antonito T. Panganiban, Ariela Samantha, Arnab K. Chatterjee, Arthur L. Haas, Ashleigh S. Paparella, Ashley L. St. John, Ashutosh Prince, Assmaa ElSheikh, Athena Apfel, Audrey Colomba, Austin T. O’Dea, Bakary N’tji Diallo, Beatriz Murta Rezende Moraes Ribeiro, Ben A. Bailey-Elkin, Benjamin Edelman, Benjamin Liou, Benjamin Perry, Benjamin Soon Kai Chua, Benjámin Kováts, Bernhard Englinger, Bijina Balakrishnan, Bin Gong, Bogos Agianian, Brandon Pressly, Brenda P. Medellin Salas, Brendan M. Duggan, Brian V. Geisbrecht, Brian Dymock, Brianna C. Morten, Bruce D. Hammock, Bruno Eduardo Fernandes Mota, B.C. Dickinson, Cameron Fraser, Camille Lempicki, Carl D. Novina, Carles Torner, Carlo Ballatore, Carlotta Bon, Carly J. Chapman, Carrie L. Partch, Catherine T. Chaton, Chang Shan Huang, Chao‐Yie Yang, Charlene M. Kahler, Charles Karan, Charles Keller, Chelsea L. Dieck, Chen Huimei, Chen Liu, Cheryl Peltier, Chinmay Kumar Mantri, Chinyere Maat Kemet, Christa E. Müller, Christian Weber, Christina M. Zeina, Christine S. Muli, Christophe Morisseau, Cigdem Alkan, Clara Reglero, Cody A. Loy, Cornelia M. Wilson, Courtney Myhr, Cristina Arrigoni, Cristina Paulino, César Santiago, Dahai Luo, Damon J. Tumes, Daniel A. Keedy, Daniel A. Lawrence, Daniel Chen, Danny Manor, Darci J. Trader, David A. Hildeman, David H. Drewry, David J. Dowling, David J. Hosfield, David M. Smith, David Moreira, David P. Siderovski, David Shum, David T. Krist, David W. H. Riches, Davide M. Ferraris, Deborah H. Anderson, Deirdre R. Coombe, Derek S. Welsbie, Di Hu, Diana Ortiz, Dina Alramadhani, Zhang Ding-qiang, Dipayan Chaudhuri, Dirk J. Slotboom, Donald R. Ronning, Donghan Lee, Dorian Dirksen, Douglas A. Shoue, Douglas W. Zochodne, Durga Krishnamurthy, Dustin Duncan, Dylan M. Glubb, Edoardo Luigi Maria Gelardi, Edward C. Hsiao, Edward G. Lynn, Elany Barbosa Silva, Elena Aguilera, Elena Lenci, Elena Theres Abraham, Eleonora Lama, Eleonora Mameli, Elisa Leung, Ellie Giles, Emily M. Christensen, Emily R. Mason, Enrico Petretto, Ephraim F. Trakhtenberg, Eric J. Rubin, Erick Strauss, Erik W. Thompson, Erika Cione, Erika M. Lisabeth, Erkang Fan, Erna Geessien Kroon, Eunji Jo, Eva M. García‐Cuesta, Evgenia Glukhov, Evripidis Gavathiotis, Yu Fang, Xiang Fei, Fenfei Leng, Feng Wang, Filippo Ingoglia, Focco van den Akker, Francesco Borriello, Franco J. Vizeacoumar, Frank Luh, Frederick S. Buckner, Frederick S. Vizeacoumar, Fredj Ben Bdira, Fredrik Svensson, G. Marcela Rodríguez, Gabriella Bognár, Gaia Lembo, Gang Zhang, Garrett Dempsey, Gary Eitzen, Gaétan Mayer, Geoffrey L. Greene, George A. Garcia, Gergely L. Lukács, Gergely Prikler, Gian Carlo G. Parico, Gianni Colotti, Gilles De Keulenaer, Gino Cortopassi, Giovanni Roti, Giulia Girolimetti, Giuseppe Fiermonte, Giuseppe Gasparre, Giuseppe Leuzzi, Gopal P. Dahal, Gracjan Michlewski, Graeme L. Conn, Grant Stuchbury, Gregory R. Bowman, Grzegorz M. Popowicz, Guido Veit, Guilherme de Souza, Gustav Akk, Guy Caljon, Guzmán Álvarez, Gwennan Rucinski, Gyeongeun Lee, Gökhan Cildir, Hai Li, Hairol E. Breton, Hamed Jafar‐Nejad, Han Zhou, Hannah P. Moore, Hannah Tilford, Haynes Yuan,
High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that ...
Nature Portfolio
Produção Nacional
Revisado por pares
Pedro Augusto Alves, Ellen G. de Oliveira, Ana Paula Moreira Franco-Luiz, Letícia Trindade Almeida, Amanda Bonoto Gonçalves, Iara A. Borges, Flávia Rocha, Raíssa Prado Rocha, Matheus Filgueira Bezerra, Pâmella Miranda, Flávio Diniz Capanema, Henrique Resende Martins, Gerald Weber, Santuza Maria Ribeiro Teixeira, Gabriel Luz Wallau, Rubens Lima do Monte‐Neto,
The coronavirus disease 2019 (COVID-19) pandemic unfolded due to the widespread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission reinforced the urgent need for affordable molecular diagnostic alternative methods for massive testing screening. We present the clinical validation of a pH-dependent colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection. The method revealed a limit of detection of 19.3 ± 2.7 viral genomic copies/ ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2021 - Frontiers Media | Frontiers in Microbiology
Produção Nacional
Revisado por pares
Marta Oliveira, Maria João Rodrigues, Catarina Pereira, Rubens Lima do Monte‐Neto, Policarpo Ademar Santos, Nuno R. Neng, J.M.F. Nogueira, João Varela, Luísa Barreira, Luísa Custódio,
This work reports for the first time the in vitro anti Leishmania infantum activity of acetone and dichloromethane (DCM) extracts from 25 extremophile plants from Southern Portugal. DCM extracts from Inula chritmoides and Spergularia rubra were active against axenic promastigotes and intracellular amastigotes, had anti-inflammatory properties on lipopolysaccharide (LPS)-stimulated macrophages, inhibited acetylcholinesterase and had no haemolytic activity on human erythrocytes. Eleven phenolics were ...
Tópico(s): Cynara cardunculus studies
2017 - Taylor & Francis | Natural Product Research