Jianmin Chai, Caleb Weiss, Paul A Beck, Wei Zhao, Ying Li, Jiangchao Zhao,
... stage, but knowledge of the dynamics of the rumen microbiome associated with the host are lacking. A ... stages of production affects the temporal dynamics of rumen microbiota. During the stocker phase, either dry hay ... received similar finishing diets with or without monensin. Rumen microbial samples were collected on d 0, 28, ... of the bacterial 16S rRNA gene of 263 rumen samples was sequenced. Results Higher alpha diversity, including ... the stocker stage temporally impact the dynamics of rumen microbiota. For example, shared bacteria, including Bacteroidales (ASV19) ...
Tópico(s): Gout, Hyperuricemia, Uric Acid
2024 - BioMed Central | Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology
Bingxing Jiang, Chaobin Qin, Yixue Xu, Xinhui Song, Yiheng Fu, Ruijia Li, Qingyou Liu, Deshun Shi,
... trait. It is unknown to what extent, the rumen microbiome and its metabolites, as well as the ... milk fat yield. Therefore, we will analyze the rumen microbiome and host-level potential regulatory mechanisms on milk yield and milk fat yield through rumen metagenomics, rumen metabolomics, and serum metabolomics experiments. Microbial metagenomics analysis ... significantly higher abundance of several species in the rumen of high-yield dairy buffaloes, which mainly belonged ... fatty acid biosynthesis and lipid metabolism. Furthermore, the rumen of high-yield dairy buffaloes exhibited a lower ...
Tópico(s): Ginseng Biological Effects and Applications
2024 - Frontiers Media | Frontiers in Microbiology
Springer Link
Jing Wang, Yuntao Dong, Wei Guo, Xiao Zhang, A. Allan Degen, Sisi Bi, Luming Ding, Xiang Chen, Ruijun Long,
... and physiologically to the Qinghai-Tibetan Plateau. The rumen microbiota has also co-evolved with the host ... the environment. Understanding the complex linkages between the rumen microbiota, the host, and the environment is essential to optimizing the rumen function to meet the growing demands for animal ... little is known about the mechanisms of host-rumen microbiome-environment linkages and how they ultimately benefit ... yaks and integrating the key aspects of its rumen microbiome with the host metabolic efficiency and homeostasis. ...
Tópico(s): Agroforestry and silvopastoral systems
2024 - Frontiers Media | Frontiers in Microbiology
Alaa Emara Rabee, M. Ghandour, A. Sallam, Eman A. Elwakeel, Rasha S. Mohammed, Ebrahim A. Sabra, A.M. Abdel-Wahed, Disouky Mohamed Mourad, Amal A. Hamed, Osama Raef Hafez,
Abstract Background Phytochemical compounds can modify the rumen microbiome and improve rumen fermentation. This study evaluated the impact of supplementation with tannin and an herbal mixture containing ginger ( Zingiber officinale ), garlic ( ... Artemisia vulgaris ), and turmeric ( Curcuma longa ) on the rumen fermentation and microbiota, and histology of rumen tissue of goats. Eighteen Shami male goats were ... Results Group HM revealed higher ( P < 0.05) rumen pH, total volatile fatty acids (VFA), acetic, propionic, ...
Tópico(s): Gout, Hyperuricemia, Uric Acid
2024 - BioMed Central | BMC Veterinary Research
Jianmin Chai, Y Zhuang, Kai Cui, Yanliang Bi, Naifeng Zhang,
... resistance. Although it has been reported that the rumen microbiome of ruminants may be a reservoir of ... the factors affecting the temporal dynamics of the rumen resistome are still unclear. Here, we collected rumen content samples of goats at 1, 7, 14, ... metagenomics, and assessed the temporal dynamics of the rumen resistome in goats at the early stage of ... conventional feeding system. Results In our results, the rumen resistome of goat kids contained ARGs to 41 ... age. A strong correlation between the profiles of rumen resistome and microbiome was found using Procrustes analysis. ...
Tópico(s): Milk Quality and Mastitis in Dairy Cows
2024 - BioMed Central | Microbiome
Abstract Background The rumen microbiome enables ruminants to digest otherwise indigestible feedstuffs, thereby facilitating the production of high-quality protein, albeit with suboptimal ... methane. Despite extensive research delineating associations between the rumen microbiome and ruminant production traits, the functional roles of the pervasive and diverse rumen virome remain to be determined. Results Leveraging a recent comprehensive rumen virome database, this study analyzes virus-microbe linkages, ...
Tópico(s): Plant Virus Research Studies
2024 - BioMed Central | Microbiome
Dangdang Wang, Luyu Chen, Guangfu Tang, Junjian Yu, Jie Chen, Zongjun Li, Yangchun Cao, Xinjian Lei, Lu Deng, Shengru Wu, Le Luo Guan, Junhu Yao,
... goats, and the contribution of the early-life rumen microbiome to lifelong growth and lactation performance in ... been well defined. Hence, this study assessed the rumen microbiome in young goats with different average daily ... 15 lowest ADG (LADG) goats were subjected to rumen fluid microbiome and metabolome profiling. The comparison of the rumen metagenome of HADG and LADG goats revealed that ... were enhanced in HADG goats, suggesting that the rumen fluid microbiome of HADG goats has higher feed ... and correlated with LADG-enriched metabolites, such as rumen acetate as well as methane. Additionally, some functional ...
Tópico(s): Reproductive Physiology in Livestock
2023 - BioMed Central | Microbiome
Dangdang Wang, Guangfu Tang, Yannan Wang, Junjian Yu, Luyu Chen, Jie Chen, Yan‐Bo Wu, Yuanjie Zhang, Yangchun Cao, Junhu Yao,
... this concept has never been used in the rumen, and little is known about the complex biological relationships between ruminants and their rumen bacterial clusters. In this study, we used young ... fed them the same diet, and analyzed their rumen microbiome and corresponding bacterial clusters. The relationships between the bacterial clusters and rumen fermentation and growth performance in the goats were ... and branched chain and saturated fatty acids in rumen fluid (P < 0.05). Several members of Firmicutes, ...
Tópico(s): Genetic and phenotypic traits in livestock
2023 - KeAi | Animal nutrition
Macey P. Soltis, Sarah E. Moorey, Amanda M. Egert-McLean, Brynn H. Voy, Elizabeth Shepherd, Phillip R. Myer,
The rumen is a complex organ that is critical for its host to convert low-quality feedstuffs into energy. ... other end products is primarily driven by the rumen microbiome and its interaction with the host. Importantly, the rumen is demarcated into five distinct rumen sacs as a result of anatomical structure, resulting in variable physiology among the sacs. However, rumen nutritional and microbiome studies have historically focused on ... or fluids sampled from single regions within the rumen. Examining the rumen microbiome from only one or ...
Tópico(s): Wheat and Barley Genetics and Pathology
2023 - Multidisciplinary Digital Publishing Institute | Microorganisms
Sijia Liu, Zhongtang Yu, Huiyue Zhong, Nan Zheng, Sharon Huws, Jiaqi Wang, Shengguo Zhao,
... ability to recycle urea-N back to the rumen where numerous ureolytic bacteria hydrolyze urea into ammonia, ... used by numerous bacteria as their nitrogen source. Rumen ureolytic bacteria are the key microbes making ruminants ... in situ agarose microsphere embedding and cultivation under rumen-simulating conditions, to isolate ureolytic bacteria from the rumen microbiome. We optimized the dilutions of the rumen microbiome during the enrichment, single-cell embedding, and ... microsphere-embedded bacteria using dialysis bags placed in rumen fluid. Metabonomic analysis revealed that the dialysis bags ...
Tópico(s): Biofuel production and bioconversion
2023 - BioMed Central | Microbiome
Ming Yan, Akbar Adjie Pratama, Sripoorna Somasundaram, Zongjun Li, Yu Jiang, Matthew B. Sullivan, Zhongtang Yu,
Abstract The diverse rumen virome can modulate the rumen microbiome, but it remains largely unexplored. Here, we mine 975 published rumen metagenomes for viral sequences, create a global rumen virome database (RVD), and analyze the rumen virome for diversity, virus-host linkages, and potential roles in affecting rumen functions. Containing 397,180 species-level viral operational ... vOTUs), RVD substantially increases the detection rate of rumen viruses from metagenomes compared with IMG/VR V3. ... from those found in the human gut. The rumen virome is predicted to infect the core rumen ...
Tópico(s): Viral gastroenteritis research and epidemiology
2023 - Nature Portfolio | Nature Communications
Jishan An, Weijun Shen, Hu Liu, Chen Yang, Kemeng Chen, Qiongwen Yuan, Zhiqing Li, Dingfu Xiao, Wang Zuo, Xinyi Lan, Lei Liu, Fachun Wan,
... study was conducted to compare the effects of rumen-protected (RP-Leu) and unprotected L -leucine (RU- ... bacterial composition, and amino acid metabolism in vitro rumen batch incubation. The 5.00 g RP-Leu ... Leu products were incubated in situ in the rumen of four beef cattle ( Bos taurus ) and removed ... 12, 16, and 24 h to determine the rumen protection rate. In in vitro incubation, both RP- ... vitro and in situ experiments confirmed that the rumen protection rate was greater ( p < 0.01) ... Leu was more stable than RU-Leu in rumen fluid, but RU-Leu also does not exhibit ...
Tópico(s): Animal Nutrition and Physiology
2023 - Frontiers Media | Frontiers in Microbiology
À. Bach, Mélody Baudon, G. Elcoso, Javier Viejo, Aurore Courillon,
... differences in dietary cation-anion difference (DCAD) on rumen pH, rumen microbial population, and milk performance of dairy cattle exposed to an induced decrease in rumen pH through a dietary challenge. Cows were randomly ... differing in the type of supplement to modulate rumen pH: (1) control, no supplementation; (2) SB, supplemented ... Seven cows per treatment were equipped with electronic rumen boluses to monitor rumen pH. Control and SB cows consumed less dry ... as dietary FCR decreased, independently of dietary treatments. Rumen pH decreased and the proportion of the day ...
Tópico(s): Animal health and immunology
2023 - Elsevier BV | Journal of Dairy Science
K.M. McCarthy, M.B. Lynch, K.M. Pierce, Alan G. Fahey, Vivian Gath, Michael McDonald, T.M. Boland, Helen Sheridan, Maria Markiewicz‐Kęszycka, F.J. Mulligan,
An experiment was conducted to compare the rumen fermentation and degradability of three forage treatments. A perennial ryegrass (PRG; Lolium perenne), perennial ryegrass and white clover (Trifolium pratense) (PRGWC) and multispecies ... intybus) and plantain (Plantago lanceolata), were compared. Six rumen fistulated lactating dairy cows were used in a ... before entering a 7 – day sampling period. Indwelling rumen pH probes were inserted into the rumen of each cow on day 15 and 16. Rumen fluid samples were taken on day 18 every ...
Tópico(s): Pasture and Agricultural Systems
2023 - Elsevier BV | Livestock Science
Claire A. Shaw, Yuna Park, María Eugenia González, Rich A. Duong, Pramod Pandey, Charles Brooke, Matthias Hess,
The rumen contains a complex mixture of microbes, crucial for the animal’s ability to degrade feed. Some of ... to the animal and improving animal productivity. Artificial rumen systems (ARSs) have been widely used to evaluate ... their ability to reduce methane production in the rumen and their effect on the rumen microbiome function prior to conducting resource-intensive animal ... different ARSs and compared them to in vivo rumen metrics. The results showed that all systems were ... over time. The batch-style ARS simulated the rumen over 48 h. The semi-continuous ARS mimicked ...
Tópico(s): Reproductive Physiology in Livestock
2023 - Multidisciplinary Digital Publishing Institute | Fermentation
Chunjia Jin, Xiaodong Su, Peiyue Wang, Ziqi Liang, Xinjian Lei, Hanxun Bai, Gaofeng Liang, Jie Li, Yangchun Cao, Junhu Yao,
... study was to investigate the effects of dietary rumen degradable starch level (RDS) on growth and carcass performance, rumen fermentation, and rumen papillae development in growing goats. Forty healthy, weaned, ... were randomly enrolled to receive either a low rumen degradable starch diet (LRDS, diet based on whole corn, RDS = 13.85 %, n = 20) or high rumen degradable starch diet (HRDS, diet based on crashed ... postprandial (P < 0.05). HRDS diet up-regulated rumen acetate proportion before morning feeding to 8 h ... decreased the length and the surface area of rumen papilla (P < 0.05). Further, HRDS diet decreased ...
Tópico(s): Genetic and phenotypic traits in livestock
2023 - Elsevier BV | Animal Feed Science and Technology
Jinsong Liang, Muhammad Zubair, Le Chen, Jianning Chang, Wei Fang, Mohammad Nabi, Wen Yang, Yajie Zhang, Yuehan Li, Panyue Zhang, Guangming Zhang, Aijie Wang,
... the problem of energy shortage and environmental pollution. Rumen microbes can improve the hydrolysis efficiency of corn ... volatile fatty acids (VFAs) efficiently. However, long-term rumen fermentation practice has not been conducted and performances ... hydrolysis and acidogenesis performances and microbial succession of rumen microbes for 120 d were explored in a ... 2%. A high VFA/SCOD ratio indicated that rumen semi-continuous reactor had a good hydrolysis and acidogenesis capacity. Diversity and abundance of rumen bacterial communities in the stable stage (after Day ...
Tópico(s): Biofuel production and bioconversion
2023 - Elsevier BV | Fuel
... Jersey dairy cows: Effects on enteric methane emission, rumen fermentation and bacterial community, digestibility, production, and feeding ... Jersey dairy cows: Effects on enteric methane emission, rumen fermentation and bacterial community, digestibility, production, and feeding ... fecal organic matter excretion (i.e., from decreased rumen or total-tract fiber digestibility due to increased ... Asparagopsis taxiformis and oregano leaves on methane emission, rumen fermentation, and lactational performance of dairy cows.J. ...
Tópico(s): Anaerobic Digestion and Biogas Production
2023 - Elsevier BV | Journal of Dairy Science
Felix Kibegwa, Rawlynce Bett, C.K. Gachuiri, Eunice Machuka, Francesca Stomeo, Fidalis D. N. Mujibi,
... on the composition and associated functional genes of rumen and fecal microbiota. We also explored fecal samples as a proxy for rumen liquor samples. Six crossbred dairy cattle were reared ... consecutive 10-day periods. After each period, individual rumen liquor and fecal samples were collected and analyzed ... compared between diets and sample types (fecal and rumen liquor). Results indicated that dietary modifications significantly affected several rumen and fecal microbial OTUs. In the rumen, an increase in dietary concentrate resulted in an ...
Tópico(s): Plant and fungal interactions
2023 - Public Library of Science | PLoS ONE
Huihui Wang, Manchun Su, Chunhui Wang, Dengpan Li, Qiao Li, Zilong Liu, Xingcai Qi, Yi Wu, Yongju Zhao, Taotao Li, Youji Ma,
... into the impact of yeast culture (YC) on rumen epithelial development, microbiota, and metabolome, with the aim of investigating YC's mechanism in regulating rumen fermentation. Thirty male lambs of Hu sheep with ... epithelial tissues were collected for histomorphological analysis, and rumen microorganisms were analyzed by 16S rDNA sequencing and rumen metabolites by untargeted liquid chromatography-mass spectrometry (LC- ... metabolomics techniques. The results showed that YC improved rumen papilla development and increased rumen papilla length (p < ...
Tópico(s): Ginseng Biological Effects and Applications
2023 - Frontiers Media | Frontiers in Microbiology
Shuli Yang, Guangrong Zhang, Zaimei Yuan, Shichun He, Rongjiao Wang, Jieyi Zheng, Huaming Mao, Jianmin Chai, Dongwang Wu,
The rumen of ruminants is inhabited by complex and diverse microorganisms. Young animals are exposed to a variety of ... sequencing of bacterial and fungal communities in the rumen of pastured yaks of different ages (from 5 ... amplified sequencing technology. The results showed that the rumen microflora of Zhongdian yaks changed gradually from 5 ... to stabilize at 2 years of age. The rumen of adult yaks was the most suitable for ... of most bacteria. Bactria diversity of the yak rumen increased gradually from 5 days after birth to ...
Tópico(s): Indigenous Studies and Ecology
2023 - Frontiers Media | Frontiers in Veterinary Science
Produção Nacional
Revisado por pares
L.L. Cunha, H.F. Monteiro, C.C. Figueiredo, Igor F. Canisso, R.C. Bicalho, F.C. Cardoso, Bart C. Weimer, F.S. Lima,
Abstract Less invasive rumen sampling methods, such as oro-esophageal tubing, became widely popular for exploring the rumen microbiome and metabolome. However, it remains unclear if such methods represent well the rumen contents from the rumen cannula technique. Herein, we characterized the microbiome and metabolome in the rumen content collected by an oro-esophageal tube and by rumen cannula in ten multiparous lactating Holstein cows. The ... pH of oro-esophageal samples was greater than rumen cannula, we found no difference in alpha and ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2023 - Nature Portfolio | Scientific Reports
S. Świerk, Marcin Przybyło, Jadwiga Flaga, Kinga Szczepanik, Aleksandra Garus‐Piętak, Weronika Biernat, Edyta Molik, Dorota Wojtysiak, Renata Miltko, P. Górka,
... is considered to have a positive impact on rumen epithelium growth and function. However, excessive ruminal butyrate production may affect the rumen negatively, particularly when the rumen is already challenged with low pH. The aim ... area, either in the atrium ruminis or ventral rumen (P ≥ 0.11). Stratum corneum thickness was higher ... 0.04), independently of the location in the rumen. In the atrium ruminis, the epithelium and living ... 03); conversely, in the ventral sac of the rumen, a thicker epithelium was observed due to both ...
Tópico(s): Animal health and immunology
2023 - Elsevier BV | animal
H.M. Golder, Jennifer M. Thomson, J. Rehberger, Alexandra H. Smith, E. Block, I.J. Lean,
... Canada, the USA, and Australia. Phenotypic observations included rumen metabolome, acidosis risk, ruminal bacterial taxa, and milk ... detergent fiber = 27 to 58% of dry matter). Rumen samples were collected <3 h after feeding and ... or hair (65 cows) collected simultaneously with the rumen samples and sequenced using the Geneseek Genomic Profiler ... tended to be associated with milk fat yield, rumen acetate, butyrate, and isovalerate concentrations and with the ... was associated or tended to be associated with rumen isobutyrate and caproate concentrations, and the center log ...
Tópico(s): Reproductive Physiology in Livestock
2023 - Elsevier BV | Journal of Dairy Science
Yongliang Huang, Guoxiu Wang, Qian Zhang, Zhanyu Chen, Chong Li, Weimin Wang, Xiaoxue Zhang, Xiaojuan Wang, Deyin Zhang, Panpan Cui, Zongwu Ma,
... However, feeding excessive MR might be detrimental to rumen development and microbial colonization. Herein, we investigated the effects of feeding different levels of MR on rumen digestive function and ruminal microorganisms. Fourteen healthy male ... feeding level on growth performance, fiber degradation rates, rumen fermentation parameters, enzyme activities and rumen histomorphology. We found that feeding with a high ... of early lambs, but decreased the starter intake, rumen weight and papillae length. We also analyzed the ...
Tópico(s): Turfgrass Adaptation and Management
2023 - Frontiers Media | Frontiers in Microbiology
Yongkang Zhen, Zanna Xi, Shaima Mohamed Nasr, Feiyang He, Mengli Han, Junliang Yin, Ling Ge, Yifei Chen, Yusu Wang, Wenjun Wei, Yihui Zhang, Mengzhi Wang,
... exogenous supplementation of butyrate in the regulation of rumen development and homeostasis. However, the effects of other ... to examine their respective roles in regulating the rumen microbiome, metabolism, and epithelium homeostasis. Data demonstrated that the infusion of sodium acetate (SA) increased rumen index while also promoting SCFA production and absorption ... concentration of occludin, and higher abundances of specific rumen bacteria, such as "Candidatus Saccharimonas," Christensenellaceae R-7, ...
Tópico(s): Plant and fungal interactions
2023 - American Society for Microbiology | Microbiology Spectrum
Wei Guo, Sisi Bi, W.W. Wang, Mi Zhou, A. L. A. Neves, A. Allan Degen, Le Luo Guan, Ruijun Long,
... the succession and establishment of the early-life rumen microbiome in newborns, but the contributions of different maternal sites to the rumen microbial establishment remain unclear. In the present study, samples from different dam sites (namely, oral, rumen fluid, milk, and teat skin) and rumen fluid of yak calves were collected at 6 ... of the bacterial and archaeal communities in the rumen during early life. Our analysis demonstrated that the ... clustered according to the sites, and the calves' rumen microbiota resembled that of the dam consistently regardless ...
Tópico(s): Animal health and immunology
2023 - Elsevier BV | Journal of Dairy Science