Raquel Caerols Mateo, Alejandro Tapia Frade,
Resumen es: Introduc ir se en la ensenanza del l en gu aje vis ua l y en la narrativa audiovisua l como hecho especifico de l len gu aj e cinematografico sup on e in...
Tópico(s): Media, Journalism, and Communication History
2013 - Universidad de Los Hemisferios | Razón y Palabra
... even been the subject of two feature films: RKO 281 (Benjamin Ross, 1999) and Mank (David Fincher, 2020), ...
Tópico(s): Digital Media Forensic Detection
2023 - Springer International Publishing | Quantitative methods in the humanities and social sciences
Sandra K. Cooper, Jui Pandhare, Steven P. Donald, James M. Phang,
... K. Phang J.M. J. Biol. Chem. 2006; 281: 2044-2052Abstract Full Text Full Text PDF PubMed Scopus (95) Google Scholar). Transfections and siRNA—The RKO cells were cultured in 6-well plates in ...
Tópico(s): Redox biology and oxidative stress
2008 - Elsevier BV | Journal of Biological Chemistry
... Zhu J. Chen X. J. Biol. Chem. 2006; 281: 2533-2542Abstract Full Text Full Text PDF PubMed Scopus (90) Google Scholar, 18Nozell S. Wu Y. McNaughton K. Liu G. Willis A. Paik J.C. Chen X. Oncogene. 2003; 22: 4333-4347Crossref PubMed Scopus (30) Google Scholar). RKO-GLX2 cell lines, which inducibly express GLX2 under ...
Tópico(s): Folate and B Vitamins Research
2006 - Elsevier BV | Journal of Biological Chemistry
Heike Allgayer, Heng Wang, Yao Wang, M. M. Heiss, Reinhard Bauer, Okot Nyormoi, Douglas Boyd,
... from a high urokinase receptor-expressing cell line (RKO) indicated that Sp1, Sp3, and a factor similar ... related factor diminished urokinase receptor promoter activity. In RKO cells, the expression of a negative regulator of ... from a high urokinase receptor-expressing cell line (RKO) indicated that Sp1, Sp3, and a factor similar ... related factor diminished urokinase receptor promoter activity. In RKO cells, the expression of a negative regulator of ... MACS-Select method of Miltenyi Biotech (Auburn, CA). RKO cells were co-transfected with the AP-2αB ...
Tópico(s): Ubiquitin and proteasome pathways
1999 - Elsevier BV | Journal of Biological Chemistry
Huei‐Min Lin, Masahiro Kaneshige, Li Zhao, Xiao‐Yong Zhang, John A. Hanover, Sheue-yann Cheng,
... Previously we have found that human colon carcinoma RKO cells exhibit strong T3-independent transcriptional activity. We therefore searched for co-regulatory proteins in RKO cells using a yeast two-hybrid system with ... carboxyl-terminal region and was abundantly expressed in RKO cells. The expressed protein localized both to the ... Previously we have found that human colon carcinoma RKO cells exhibit strong T3-independent transcriptional activity. We therefore searched for co-regulatory proteins in RKO cells using a yeast two-hybrid system with ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2001 - Elsevier BV | Journal of Biological Chemistry
Wengong Wang, Xiaoling Yang, Tomoko Kawai, Isabel López de Silanes, Krystyna Mazan-Mamczarz, Peili Chen, Yuh Min Chook, Christina Quensel, Matthias Köhler, Myriam Gorospe,
... Culture, Treatment, Transfection, and Infection—Human colorectal carcinoma RKO cells were cultured in minimum essential medium (41Gorospe ... s medium for 4 h. Infection efficiency of RKO cells was determined by infection with AdGFP at ... in accordance with the low infection rates of RKO cells (41Gorospe M. Wang X. Guyton K.Z. ... units/cell were used in all infections of RKO and 293 cells, respectively. Cytoplasmic, nuclear, and whole ... with IP buffer. Pulse Labeling of Endogenous Protein—RKO cells (200,000 cells/6-cm dish) were ...
Tópico(s): RNA modifications and cancer
2004 - Elsevier BV | Journal of Biological Chemistry
Yingjuan Qian, Jin Zhang, Bingfang Yan, Xinbin Chen,
... DEC1-XhoI as shown above. Cell Lines—MCF7, RKO, MCF7-p53-KD, and RKO-p53-KD were cultured in Dulbecco's modified ... Scopus (30) Google Scholar). MCF7-p53-KD and RKO-p53-KD are derivatives of MCF7 and RKO, respectively, in which p53 was stably knocked down ... gene. To this end, MCF7, MCF7-p53-KD, RKO, and RKO-p53-KD cells were untreated or treated with ... DEC1 was induced by doxorubicin in MCF7 and RKO cells (Fig. 1B, DEC1 panel). Similarly, p21 was ... p21 was detected in p53-knockdown MCF7 and RKO cells (Fig. 1B, DEC1 and p21 panels). Next, ...
Tópico(s): DNA Repair Mechanisms
2007 - Elsevier BV | Journal of Biological Chemistry
... the authors also demonstrate that the HDAC2-deficient RKO colon cancer cell line is highly refractory to ... APAF1), a proapoptotic protein, specifically in HDAC2-deficient RKO cells, establishing a link between APAF1 induction and ... levels of APAF1 were elevated in HDAC2 mutant RKO cells. Based on these findings, the authors propose ... PubMed Scopus (13) Google Scholar report that the RKO cell line cultured in their laboratory does express HDAC2, as do RKO cells obtained directly from the American Type Culture ...
Tópico(s): Ubiquitin and proteasome pathways
2008 - Elsevier BV | Gastroenterology
Long Shan Li, Julio C. Morales, Arlene Hwang, Mark W. Wagner, David A. Boothman,
... mammalian cytomegalovirus expression vector, and used to transfect RKO or HCT116 cells as indicated (14Davis T.W. ... Correlates with Apoptosis in MNNG-treated MMR-corrected RKO Cells—Human RKO cells are MMR-deficient due to hMLH1 promoter ... 59: 3021-3027PubMed Google Scholar); however, we selected RKO clones that express hMLH1 at levels comparable to ... cells was critical for long-term stability. Various RKO clones were established, and all showed increased sensitivity ... more years in culture, unlike previous reports of RKO clones in which hMLH1 expression was driven to ...
Tópico(s): Cholangiocarcinoma and Gallbladder Cancer Studies
2008 - Elsevier BV | Journal of Biological Chemistry
Eike Gallmeier, Eric S. Calhoun, Carlo Rago, Jonathan R. Brody, Steven C. Cunningham, Tomáš Hucl, Myriam Gorospe, Manu Kohli, Christoph Lengauer, Scott E. Kern,
... and FANCG in the human adenocarcinoma cell line RKO. The generation of naturally immortal, isogenic human cancer ... CA). pAAV, pRC, and pHelper were cotransfected into RKO cells using Lipofectamine (Invitrogen, Carlsbad, CA). Hygromycin-resistant ... cell population and chromosome. We disrupted FANCG in RKO cells by serially deleting the 2 alleles of ... Two FANCG+/− and 3 FANCG−/− clones were obtained. RKO cells contain an unbalanced translocation (der20)t(9; ... numbers reflect methodologic background levels previously reported for RKO cells (≤10%).19Lengauer C. Kinzler K.W. Vogelstein ...
Tópico(s): DNA and Nucleic Acid Chemistry
2006 - Elsevier BV | Gastroenterology
Tracy M. Wagner, James E. Mullally, F.A. Fitzpatrick,
... covalent adduct with LKB1 in MCF-7 and RKO cells. Site-directed mutagenesis implicated Cys210 in the ... covalent adduct with LKB1 in MCF-7 and RKO cells. Site-directed mutagenesis implicated Cys210 in the ... mg/ml gentamicin, and 10% fetal bovine serum. RKO colon cancer cells were maintained in minimal essential ... Covalently Labeled by PG-Amidopentylbiotin—MCF-7 and RKO cells were treated with 10–60 μm PGA1- ... HA or LKB1 (C210S)-HA was transfected into RKO cells using 1 μg/μl DNA, 3 μl ... of LKB1 is required for lipid adduct formation. RKO cells express negligible amounts of LKB1; therefore, we ...
Tópico(s): Cancer, Lipids, and Metabolism
2005 - Elsevier BV | Journal of Biological Chemistry
... bovine serum and penicillin/streptomycin. Human colorectal carcinoma RKO cells were grown on plates in Dulbecco's ... as substrates for 30 min at 30 °C. RKO cells were transfected with 3 μg of either ... imaging densitometer. FLAG-N- and FLAG-C-SSRP1 RKO cell lysates were used to perform co-IPs ... N- and C-SSRP1 in human colorectal carcinoma RKO cells and performed co-immunoprecipitations (co-IPs) with ... cand e), FLAG-C-SSRP1 stably expressed in RKO cells migrates slower than FLAG-N-SSRP1 (Fig. ...
Tópico(s): Epigenetics and DNA Methylation
2002 - Elsevier BV | Journal of Biological Chemistry
Chang Guo, Yu Wang, David L. Brautigan, James M. Larner,
... be an important target of damage sensing pathways. RKO cells, derived from a human colon cancer, were ... Roche Molecular Biochemicals (Meylan, France). Asynchronous Jurkat and RKO cells were exposed to various doses of radiation ... the kinetics of H1 dephosphorylation, asynchronous Jurkat and RKO cells were radiated with 5 Gy, and samples ... was no decrease in total H1 in either RKO or Jurkat cells due to IR. The dephosphorylation ... induced dephosphorylation of H1. As with Jurkat and RKO cells (Fig. 1), IR does not decrease total ...
Tópico(s): DNA Repair Mechanisms
1999 - Elsevier BV | Journal of Biological Chemistry
Xiaohua Shi, Yan-Qing Ma, Yizeng Tu, Ka Chen, Shan Wu, Koichi Fukuda, Jun Qin, Edward F. Plow, Chuanyue Wu,
... 1080 cells were from American Type Culture Collection. RKO, HT-29, DLD-1, LoVo, and HCT-116 ... University). The SK-LMS-1, HT-1080, and RKO cells were cultured in minimum Eagle's medium ... values <0.05 were considered statistically significant. For RKO cells, the cells were infected with β-galactosidase ... above. In some experiments, SK-LMS-1 and RKO cells were preincubated with function-blocking mouse anti- ... To assess FA formation, SK-LMS-1 or RKO cells infected with the control β-galactosidase adenovirus ...
Tópico(s): Galectins and Cancer Biology
2007 - Elsevier BV | Journal of Biological Chemistry
Laurent Prévotat, Rodolphe Filomenko, Éric Solary, Jean‐François Jeannin, Ali Bettaı̈eb,
... Laboratories (West Grove, PA). The human (SW480, HCT116, RKO, and HT29) cells, the murine (CT26) colorectal cancer ... glutamine at 37°C in a dry atmosphere. RKO, HCT 116, HT29, and CT26 cells were cultured ... cells, and we expressed pGL3-OT plasmid in RKO cells that do not express β-catenin (Figure ...
Tópico(s): Ubiquitin and proteasome pathways
2006 - Elsevier BV | Gastroenterology
Valérie Martin, Helle F. Jørgensen, Audrey S. Baur Chaubert, Jennifer Berger, Helen Barr, Phillip Shaw, Adrian Bird, Pascal Chaubert,
... was stably knocked down by RNA interference in RKO, a colon cancer cell line in which both < ... p14</i><sup>ARF</sup> gene in the RKO colon cancer cell line. Depletion of MBD2 by ... by expressing mouse MBD2 protein in MBD2-deficient RKO cells. <i>Conclusion:</i> These findings implicate MBD2 ...
Tópico(s): RNA modifications and cancer
2008 - Karger Publishers | Pathobiology
Wenjing Ruan, Shuzhen Zhu, Haibing Wang, Fangying Xu, Hong Deng, Yu Ma, Maode Lai,
... vitro, transfection of PcDNA3.1(IGFBP-rP1) into RKO, SW620 and CW2 cells induced a more pronounced ... genes were detected in PcDNA3.1(IGFBP-rP1)-RKO transfectants, using Affymetrix 133 plus 2.0 expression ... and immediate early response 5-like(IER5L) in RKO, SW620 and CW2 colon cancer cells, verified by ...
Tópico(s): Digestive system and related health
2010 - BioMed Central | Molecular Cancer
Heping Yang, Ainhoa Iglesias–Ara, Nathaniel Magilnick, Meng Xia, Komal Ramani, Hui Chen, Taunia D. Lee, José M. Mato, Shelly C. Lu,
... Human Research Review Committee. HepG2, HuH-7, and RKO cells were grown according to instructions provided by ... material online at www.gastrojournal.org). HepG2 and RKO cells were grown to 60%–70% confluency before ... the mitochondrial death pathway.J Biol Chem. 2006; 281: 15258-15267Crossref PubMed Scopus (193) Google Scholar We ...
Tópico(s): RNA regulation and disease
2007 - Elsevier BV | Gastroenterology
M. Saeed Sheikh, France Carrier, Mathilda A. Papathanasiou, M. Christine Hollander, Qimin Zhan, Kelly J. Yu, Albert J. Fornace,
... colony-forming efficiency of Chinese hamster ovary and RKO human colon carcinoma cells (12Hollander M.C. Alamo ... human cell lines: MCF-7, breast carcinoma cells; RKO, colon carcinoma cells; A549, lung carcinoma cells; H1299, ... Maruyama I. Suzuki K. J. Biochem. 1988; 103: 281-285Crossref PubMed Scopus (46) Google Scholar). The DDI ...
Tópico(s): RNA Interference and Gene Delivery
1997 - Elsevier BV | Journal of Biological Chemistry
Lidija Klampfer, Jie Huang, Georgia Corner, John M. Mariadason, Diego Arango, Takehiko Sasazuki, Senji Shirasawa, Leonard H. Augenlicht,
... type (WT) Ki-ras (Caco-2, Colo201, HT29, RKO, T84, and WiDr). cDNA microarray analysis was performed ... cell lines with WT p53 (HCT116, Lovo, LS174T, RKO, and SW48) and MT p53 (16E, 19A, Colo205, ...
Tópico(s): NF-κB Signaling Pathways
2003 - Elsevier BV | Journal of Biological Chemistry
Janet Y. Leung, Frank T. Kolligs, Rong Wu, Yali Zhai, Rork Kuick, Samir Hanash, Kathleen R. Cho, Eric R. Fearon,
... human colon cancer lines, except for HT29, LS174T, RKO, and SW48 cells, were grown in Dulbecco's ... L15 medium (Invitrogen) in the absence of CO2. RKO, HT29, HT29/β-Gal, and HT29/APC cells ...
Tópico(s): Kruppel-like factors research
2002 - Elsevier BV | Journal of Biological Chemistry
Weiting Ge, Hanguang Hu, Kefeng Ding, Lifeng Sun, Shu Zheng,
... the transfection of the ST14 gene in the RKO cell line. Our results also suggested that ST14 ... the dependence of TMEFF1 expression on ST14 in RKO cells. Finally, homology modeling studies suggested that TMEFF1 ...
Tópico(s): Amyloidosis: Diagnosis, Treatment, Outcomes
2006 - Elsevier BV | Journal of Biological Chemistry
Tammy L Romanuik, Takeshi Ueda, Nhu Le, Simon Haile, Theresa M.K. Yong, Thomas A. Thomson, Robert L. Vessella, Marianne D. Sadar,
... modified Eagle's medium (PC-3, DU145, and RKO), BRFF-HPC1 medium (MDA PCa 2b), or minimal ... lines that included the following: MG63, osteosarcoma cells; RKO, colon carcinoma cells; HEPG, hepatocellular carcinoma cells; MCF7, ...
Tópico(s): Cancer-related gene regulation
2009 - Elsevier BV | American Journal Of Pathology
... MD. 21. Pablo León Aguinaga, El cine norteamericano, 281–316. 22. By the end of World War II, six of the big eight Hollywood companies had local affiliates in Spain: Universal Pictures; 20th Century Fox; Loew's, Warner Brothers, Columbia and RKO. 23. Ralph Ackerman (Commercial Attache, US Embassy) to ...
Tópico(s): Cinema and Media Studies
2009 - Routledge | Historical Journal Of Film Radio and Television
Jin-Woo Ahn, Marshall Urist, Carol Prives,
... fetal bovine serum. For RNAi experiments, HCT116 and RKO (p53 wild type human colorectal adenocarcinoma cells) and ...
Tópico(s): Microtubule and mitosis dynamics
2003 - Elsevier BV | Journal of Biological Chemistry
Keiji Suzuki, Seiji Kodama, Masami Watanabe,
... the ATM protein fragment containing this motif in RKO cells abrogated the S-phase check point and ...
Tópico(s): Radiation Therapy and Dosimetry
1999 - Elsevier BV | Journal of Biological Chemistry
Lawrence H. Cohn, Gregory S. Couper, Sary F. Aranki, Robert J. Rko, David Adams, John J. Collins,
The myxomatous degenerated, prolapsed, or floppy mitral valve is the most common etiology of mitral regurgitation in North American populations. We performed mitral valve reconstruction for this diagnosis in 252 patients from 1984 to 1993. There were 165 males and 87 females ranging in age from 23 to 84 years (mean 64 years); 93 (37%) were > or = 70 years. One hundred eighty-six were New York Heart Association Functional Class III or IV and 29% (72) underwent concomitant coronary bypass operation. Operations ...
Tópico(s): Infective Endocarditis Diagnosis and Management
1994 - Wiley | Journal of Cardiac Surgery
... idea, KLF4 levels remain very low in the RKO colon cancer cell line, which contains wild-type ... EMBO J. 1998; 17: 5129-5140Crossref PubMed Scopus (281) Google Scholar). As preliminary data show that genetic ...
Tópico(s): Genetic Syndromes and Imprinting
2001 - Elsevier BV | Journal of Biological Chemistry
Jui Pandhare, Sandra K. Cooper, James M. Phang,
... cell line repository. Two colon cancer cell lines RKO and LoVo and the HEK293 cell line were ...
Tópico(s): Lipid metabolism and biosynthesis
2005 - Elsevier BV | Journal of Biological Chemistry