Lifang Wu, Yi Pan, Guoqiang Chen, Hiroaki Matsunami, Hanyi Zhuang,
... expressed in cultured cells of non-olfactory origins. RTP1S is an accessory protein to mammalian ORs and ... responses in heterologous cells. The mechanism by which RTP1S promotes the functional expression of ORs remains poorly ... a better understanding of the role(s) of RTP1S, we performed a series of structure-function analyses of RTP1S in HEK293T cells. By constructing RTP1S deletion and chimera series and subsequently introducing single- ... the protein, we found the N terminus of RTP1S is important for the endoplasmic reticulum exit of ...
Tópico(s): Neurobiology and Insect Physiology Research
2012 - Elsevier BV | Journal of Biological Chemistry
Yosuke Fukutani, Ryohei Tamaki, Ryosuke Inoue, Tomoyo Koshizawa, Shuto Sakashita, Kentaro Ikegami, Ikuroh Ohsawa, Hiroaki Matsunami, Masafumi Yohda,
Receptor-transporting protein 1S (RTP1S) is an accessory protein that mediates the transport of mammalian odorant receptors (ORs) into the plasma membrane. Although most ORs ... of ORs is achieved with the coexpression of RTP1S. However, the mechanism for RTP1S-mediated OR trafficking remains unclear. In this study, ... the mode of action and critical residues of RTP1S in OR trafficking. Experiments using N-terminal truncation and Ala substitution mutants of RTP1S demonstrated that four N-terminal amino acids have ...
Tópico(s): Neurobiology and Insect Physiology Research
2019 - Elsevier BV | Journal of Biological Chemistry
Teng Yu, Xubo Su, Yi Pan, Hanyi Zhuang,
Receptor transporting protein (RTP) family members, RTP1S and RTP2, are accessory proteins to mammalian odorant receptors (ORs). They are expressed in the olfactory sensory neurons and facilitate OR trafficking ... heterologous cells. We previously identified different domains in RTP1S that are important for different stages of OR ... we attempted to dissect the functional differences between RTP1S and RTP2 using a HEK293T cell-based OR ... ORs were tested against 28 cognate ligands, unlike RTP1S, which always showed a robust ability to support ...
Tópico(s): Neurobiology and Insect Physiology Research
2017 - Public Library of Science | PLoS ONE
Xiaoyu Qiang, Xingshao Liu, Xiaoxue Wang, Qing Zheng, Lijuan Kang, Xianxian Gao, Yushu Wei, Wenjie Wu, Hong Zhao, Weixing Shan,
... plant susceptibility factor Resistance to Phytophthora parasitica 1 (RTP1) is involved in ER stress sensing and rtp1-mediated resistance against P. parasitica is synergistically regulated ... ER stress-associated immune genes in Arabidopsis thaliana rtp1 mutant plants during the infection by P. parasitica. We further demonstrate RTP1 contributes to stabilization of the ER membrane-associated ... to P. parasitica infection. Taken together, we demonstrate RTP1 may exert negative modulating roles in the activation ...
Tópico(s): Plant Disease Resistance and Genetics
2021 - Oxford University Press | PLANT PHYSIOLOGY
Thomas G. Mast, Jessica H. Brann, Debra Ann Fadool,
... scaffolding proteins, the chaperones receptor transporting protein 1 (RTP1) and receptor expression enhancing protein 1 (REEP1) were also expressed. RTP1 was localized broadly across the VNO sensory epithelium, ... cells, and the soft palate. Both Homer and RTP1 formed protein-protein interactions with TRPC2 in native reciprocal pull-down assays and RTP1 increased surface expression of TRPC2 in in vitro assays. The RTP1-dependent TRPC2 surface expression was paralleled with an ...
Tópico(s): Neurobiology and Insect Physiology Research
2010 - BioMed Central | BMC Neuroscience
S. Buschbaum, O. Fursenko, D. Bolze, D. Wolansky, V. Melnik, J. Nieß, W. Lerch,
... on (100) Si substrates. The first RTP step (RTP1) was performed by isochronal annealing at various temperatures ... resistance (Rs) values after the first RTP step (RTP1 process window) is smaller for the Co/TiN ... and its uniformity for both systems if the RTP1 temperature was above 470 ^oC. For the Co/ ... final Rs results are not influenced by the RTP1 temperature or its uniformity (above 470 ^oC). In ... Co in a manner that depends on the RTP1 temperature and the Ti cap thickness.
Tópico(s): Semiconductor Quantum Structures and Devices
2004 - Elsevier BV | Microelectronic Engineering
Zhongdong Lin, Zhenwei Liu, Xiucui Li, Li Feng, Ying Hu, Bingyu Chen, Zhen Wang, Yong Liu,
... two deleterious de novo mutations in DYNC1H1 and RTP1 in two trios. Expression profile analysis showed that DYNC1H1 and RTP1 are expressed in almost all brain regions and ... genetic interaction network analyses suggested that DYNC1H1 and RTP1 are tightly associated with known epilepsy genes. Furthermore, ... results demonstrate DYNC1H1 as a strong candidate and RTP1 as a potential candidate on the onset of ...
Tópico(s): RNA and protein synthesis mechanisms
2017 - Nature Portfolio | Scientific Reports
Lorena Halty-deLeon, Fabio Miazzi, Sabine Kaltofen, Bill S. Hansson, Dieter Wicher,
... cells with a truncated form of the mouse RTP1 (RTP1S) or with the Drosophila sensory neuron membrane protein ... Moreover, the ability of SNMP1 to mimic the RTP1S effect may indicate possible new roles of this ...
Tópico(s): Invertebrate Immune Response Mechanisms
2016 - Elsevier BV | Journal of Neuroscience Methods
Linna Liu, Zhiwei Guo, Yan Zhang, Hua Qin, Han Yan,
... purified fraction 1 polysaccharide extracted from Rheum tanguticum RTP1 on irradiation-induced immune damage in mice.Kunming ... groups: normal group (NC), irradiation control group (IC), RTP1 low dose (200 mg/kg), middle dose (400 ... kg) and high dose (800 mg/kg) groups. RTP1 was administered by the gastric route for 14 ... markedly (P < 0.05 or P < 0.05).RTP1 has an obvious protective effects on damage in ...
Tópico(s): Ginseng Biological Effects and Applications
2012 - West Asia Organization for Cancer Prevention | Asian Pacific Journal of Cancer Prevention
Zeyu Lv, Mingming Zhao, Wenjing Wang, Qi Wang, Mengqi Huang, Chaoqun Li, Qichao Lian, Jin‐Qiu Xia, Ji Qi, Cheng‐Bin Xiang, Huiru Tang, Xiaochun Ge,
... largely unexplored. Here, we report the characterization of rtp1 (resistant to paraquat 1), an Arabidopsis mutant showing ... the widely used herbicides paraquat and diquat. The rtp1 mutant is semi-dominant and carries a point ... inhibitor ConA reduced the paraquat resistance of the rtp1 mutant. Paraquat release and uptake assays demonstrated that ...
Tópico(s): Photosynthetic Processes and Mechanisms
2021 - Elsevier BV | Molecular Plant
Chen Le, Le Shi, Yue Ma, Chunquan Zheng,
... TNF signaling pathway. The top 10 hub genes (Rtp1, Rps27a, Penk, Cxcl2, Gng8, Gng3, Cxcl1, Cxcr2, Ccl9, ... PCR validation of the five genes of interest (Rtp1, Rps27a, Penk, Cxcl2, and Anxa1), the expression level of Rtp1 mRNA was significantly decreased in the AR group ...
Tópico(s): Olfactory and Sensory Function Studies
2020 - Frontiers Media | Frontiers in Genetics
Ruchira Sharma, Yoshiro Ishimaru, Ian G. Davison, Kentaro Ikegami, Ming‐Shan Chien, Helena You, Quiyi Chi, Momoka Kubota, Masafumi Yohda, Michael Ehlers, Hiroaki Matsunami,
... OR trafficking and determining OR gene choice probabilities. Rtp1 and Rtp2 double knockout mice (RTP1,2DKO) show OR trafficking defects and decreased OSN ... despite the presence of fewer total OSNs in RTP1,2DKO. Unlike typical ORs, some overrepresented ORs show ...
Tópico(s): Neurobiology and Insect Physiology Research
2017 - eLife Sciences Publications Ltd | eLife
Chen Lin, Deying Yang, Xiaobin Gu, Xuerong Peng, Guangyou Yang,
... on the expression of the recombinant fusion protein (rTp1) from the Tp1 gene. Rapid amplification of cDNA ... pisiformis cysticercosis antisera. This purified recombinant fusion protein, rTp1, was probed by Dot-ELISA with sera from ... Dot-ELISA assay utilizing the recombinant fusion protein, rTp1, has a strong potential for the effective diagnosis ...
Tópico(s): Toxoplasma gondii Research Studies
2014 - Elsevier BV | Veterinary Parasitology
... surgical cavity observed on the sim-CT1 (boost RTP1).The volume reduction ratios were 14.4%-51. ... 0.001).The normal brain volumes in boost RTP1 were significantly reduced in boost RTP2, especially at ... CT2 which was not covered with the boost RTP1, developed in five cases (26.3%).The surgical ...
Tópico(s): Medical Imaging Techniques and Applications
2013 - Korean Academy of Medical Sciences | Journal of Korean Medical Science
Risa Nakase‐Richardson, Mark Sherer, Ronald T. Seel, Tessa Hart, Robin A. Hanks, Juan Carlos Arango‐Lasprilla, Stuart A. Yablon, Angelle M. Sander, Scott D. Barnett, William C. Walker, Flora M. Hammond,
... associated with return to productivity at 1 year (RTP1). Age was significantly associated with RTP1; however, younger patients had a different association than ... adjustment for individually significant variables, the odds of RTP1 decrease by 14% with every additional week of ...
Tópico(s): Traumatic Brain Injury Research
2011 - BMJ | Journal of Neurology Neurosurgery & Psychiatry
Hanyi Zhuang, Hiroaki Matsunami,
... we have shown that a shorter form of RTP1, RTP1S, supports robust cell-surface and functional expression of ... receptors. Using a combination of accessory proteins, including RTP1S, Ric8b, and G αolf , a diverse set of ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2007 - Elsevier BV | Journal of Biological Chemistry
Qiaona Pan, Beimi Cui, Fengyan Deng, Junli Quan, Gary J. Loake, Weixing Shan,
... to P. parasitica . Moreover, an RTP 1 mutant, rtp1‐1 , displayed localized cell death, increased reactive oxygen ... Col‐0, in response to P. parasitica infection. rtp1‐1 showed a similar disease response to the ...
Tópico(s): Plant Disease Resistance and Genetics
2015 - Wiley | New Phytologist
Blythe D. Shepard, Niranjana Natarajan, Ryan J. Protzko, Omar W. Acres, Jennifer L. Pluznick,
... and/or chaperones (i.e. Receptor Transporting Protein, RTP1S, Ric8b and Gαolf) to improve surface expression. However, ... Rho were co-expressed with previously reported chaperones (RTP1S, Ric8b and Gαolf), we observed surface expression for ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2013 - Public Library of Science | PLoS ONE
... O novo PNR', 15 June 2006. Public television RTP1 has broadcast a programme titled A Extrema‐direita ... the debate about immigration on the public channel RTP1, in the programme Prós e Contras, 15 December ...
Tópico(s): Diaspora, migration, transnational identity
2007 - Taylor & Francis | Representation
Harumi Saito, Momoka Kubota, Richard W. Roberts, Qiuyi Chi, Hiroaki Matsunami,
... surface. Here we show that the transmembrane proteins RTP1 and RTP2 promote functional cell surface expression of ... a third protein, REEP1. These findings suggest that RTP1 and RTP2 in particular play significant roles in ...
Tópico(s): Neurobiology and Insect Physiology Research
2004 - Cell Press | Cell
... 2002 and March 2003 for TVI, SIC and RTP1 channels. We divided the nutritional food claims in ... programmes (28,5% TVI, 38% SIC, 33,5 % RTP1) with a media of 6 food commercials an ...
Tópico(s): Educational Methods and Impacts
2004 - Lippincott Williams & Wilkins | Journal of Pediatric Gastroenterology and Nutrition
Xianxian Gao, Ya-ling TANG, Qing-yao SHI, Yushu Wei, Xiao-xue WANG, Weixing Shan, Xiaoyu Qiang,
... previous studies identified Resistance to Phytophthora parasitica 1 (RTP1) as a negative regulator of Arabidopsis resistance to multiple biotrophic pathogens and RTP1 loss-of-function plants displayed rapid cell death ... study, we aim to decipher the mechanism of RTP1-mediated cell death, and identified a member of ... processing enzymes (VPEs), γVPE, playing a role in rtp1-mediated resistance to P. parasitica and cell death ... 1-like protease activity in P. parasitica-infected rtp1 mutant plants. Besides, we found that the VPE/ ...
Tópico(s): Plant Pathogenic Bacteria Studies
2022 - Elsevier BV | Journal of Integrative Agriculture
Ana Cabrera, Carla Martins, Isabel Ferin Cunha,
... por blocos de jornais televisivos dos canais generalistas RTP1, SIC e TVI. Utilizamos uma metodologia fundada na ...
Tópico(s): Communication and COVID-19 Impact
2020 - Coimbra University Press | Media & Jornalismo
António Manuel Dinis Ribeiro Marques, Beatriz Lobo Moreira, Joana Cunha, Sofia Moreira,
... obtained by the designers, including in the television (RTP1) and several websites. A more conscious fashion consumer ...
Tópico(s): Consumer Packaging Perceptions and Trends
2019 - Elsevier BV | Procedia CIRP
... the television news programs of three main broadcasters – RTP1, SIC and TVI. For this purpose, we have ...
Tópico(s): Media, Gender, and Advertising
2018 - University of Beira Interior | Estudos em Comunicação
Franziska Noe, Tim Frey, Julia Fiedler, Christiane Geithe, Bettina Nowak, Dietmar Krautwurst,
... coupled receptors. Accessory proteins, such as the chaperone RTP1S, or Ric8b, have improved the surface expression of ...
Tópico(s): Neurobiology and Insect Physiology Research
2017 - POL Scientific | Journal of Biological Methods
Isidró Ferrer, Paula Garcia‐Esparcia, Margarita Carmona, Eva Carro, Eleonora Aronica, Gábor G. Kovács, Alice Grison, Stefano Gustincich,
... OR transporters Receptor Transporter Proteins 1 and 2 (RTP1 and RTP2), Receptor Expression Enhancing Protein 1 (REEP1), ...
Tópico(s): Advanced Chemical Sensor Technologies
2016 - Frontiers Media | Frontiers in Aging Neuroscience
Jaclyn Bubnell, Sophie Jamet, Delia Tomoiaga, Charlotte D’Hulst, Konstantinos Krampis, Paul Feinstein,
... even in the presence of proposed accessory proteins RTP1S or mβ2AR. To ask if ORs contain an ...
Tópico(s): Neurobiology and Insect Physiology Research
2015 - Public Library of Science | PLoS ONE
Nuno Felipe Almeida, Susana T. Leitão, Nicolas Krezdorn, Björn Rotter, Peter Winter, Diego Rubiales, María Carlota Vaz Patto,
... both inoculated libraries, as e.g. the rust Rtp1 transcript, may be responsible for the down-regulation ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
2014 - BioMed Central | BMC Plant Biology
Benjamin Pêtre, David L. Joly, Sébastien Duplessis,
... proteins have been described: AvrP123, AvrP4, AvrL567, AvrM, RTP1, and PGTAUSPE-10-1. Although some are well ...
Tópico(s): Plant Pathogens and Resistance
2014 - Frontiers Media | Frontiers in Plant Science