Marija Stanišić, Nikolina Popović Kokar, Predrag Ristić, Ana Marija Balaž, Miloš Ognjanović, Veljko Đokić, Radivoje Prodanović, Tamara R. Todorović,
Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites with higher activity were obtained, ...
Tópico(s): Chromium effects and bioremediation
2022 - Multidisciplinary Digital Publishing Institute | Polymers
Marija Stanišić, Nikolina Popović Kokar, Predrag Ristić, Ana Marija Balaž, Milan Senćanski, Miloš Ognjanović, Veljko Đokić, Radivoje Prodanović, Tamara R. Todorović,
Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein's surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite ...
Tópico(s): Enzyme Production and Characterization
2021 - Multidisciplinary Digital Publishing Institute | Polymers