Produção Nacional
Revisado por pares
Maira Alves Pereira, Vanessa Carla Furtado Mosqueira, Vildete Aparecida Sousa Carmo, Cristiano S. Ferrari, Eduardo C. O. Reis, Gilson Andrade Ramaldes, Valbert Nascimento Cardoso,
To evaluate the ability of polymeric nanocapsules (NCs) radiolabeled with technetium-99m D,L-hexamethylpropyleneamine oxime (Tc-HMPAO) to identify inflammatory process in an experimental model.NCs were prepared by interfacial deposition of preformed biodegradable polymer [poly (D,L-lactic acid) (PLA) and PLA-PEG (polyethyleneglycol)] followed by a solvent displacement. The size and homogeneity, and zeta potential of the NC preparations were determined in a Zetasizer by photon correlation spectroscopy ...
Tópico(s): RNA Interference and Gene Delivery
2009 - Lippincott Williams & Wilkins | Nuclear Medicine Communications
Produção Nacional
Revisado por pares
Vildete Aparecida Sousa Carmo, Cristiano S. Ferrari, Eduardo C. O. Reis, Gilson Andrade Ramaldes, Maira Alves Pereira, Mônica Cristina Oliveira, Valbert Nascimento Cardoso,
To investigate the biodistribution and the ability of stealth pH-sensitive liposomes radiolabelled with 99mTc to identify inflammatory regions in a rat focal inflammation model.Preformed glutathione-containing stealth pH-sensitive liposomes were labelled with 99mTc-hexamethylpropylene amine oxime (99mTc-HMPAO). The 99mTc-HMPAO radiolabelled stealth pH-sensitive liposomes (99mTc-SpHL) were administered intravenously in Wistar male rats with inflammation induced by injection subplantar of carrageenan ...
Tópico(s): Bone and Joint Diseases
2008 - Lippincott Williams & Wilkins | Nuclear Medicine Communications
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Maria Ângela de Barros Correia Menezes, Raquel Gouvêa dos Santos, Gilson Andrade Ramaldes,
Tópico(s): Radiation Therapy and Dosimetry
2010 - Springer Science+Business Media | Journal of Radioanalytical and Nuclear Chemistry
Produção Nacional
Revisado por pares
Guilherme Carneiro, Délia Chaves Moreira dos Santos, Mônica Cristina Oliveira, Ana Paula Fernandes, Luciana Souto Ferreira, Gilson Andrade Ramaldes, E. A. Nunan, Lucas Antônio Miranda Ferreira,
The present study aimed to evaluate the potential of liposomes loaded with paromomycin (PA), an aminoglycoside antibiotic associated with poor skin penetration, for the topical treatment of cutaneous leishmaniasis (CL). Fluid liposomes were prepared and characterized for particle size, zeta potential, and drug entrapment. Permeation studies were performed with two in vitro models: intact and stripped skin. The antileishmanial activity of free and liposomal PA was evaluated in BALB/c mice infected ...
Tópico(s): Insect and Pesticide Research
2010 - Taylor & Francis | Journal of Liposome Research
Produção Nacional
Revisado por pares
Cristiane dos Santos Giuberti, Eduardo Cássio de Oliveira Reis, Talita Guieiro Ribeiro Rocha, Elaine Amaral Leite, Rodrigo G. Lacerda, Gilson Andrade Ramaldes, Mônica Cristina Oliveira,
Long-circulating and pH-sensitive liposomes, containing cisplatin (SpHL-CDDP), have been developed as an alternative aimed at avoiding severe side effects as well as the appearance of resistance, which can limit the use of free cisplatin. However, physical (i.e., aggregation/fusion) and chemical instabilities limit the use of these drug carriers as pharmaceutical products. The preparation of freeze-dried pharmaceuticals has proven to be a successful strategy implemented to improve the stability of ...
Tópico(s): Drug Solubulity and Delivery Systems
2010 - Taylor & Francis | Journal of Liposome Research
Produção Nacional
Revisado por pares
Álvaro D. Carvalho Júnior, F.P. Vieira, V.J. De Melo, Míriam Teresa Paz Lopes, Josianne Nicácio Silveira, Gilson Andrade Ramaldes, Arlette Garnier‐Suillerot, Elene C. Pereira‐Maia, Mônica Cristina Oliveira,
We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl ...
Tópico(s): Drug Transport and Resistance Mechanisms
2007 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Produção Nacional
Revisado por pares
Maira Alves Pereira, Vanessa Carla Furtado Mosqueira, José Mário Carneiro Vilela, Margareth Spangler Andrade, Gilson Andrade Ramaldes, Valbert Nascimento Cardoso,
The present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use ...
Tópico(s): Orthopaedic implants and arthroplasty
2007 - Elsevier BV | European Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Mônica Cristina Oliveira, Raquel Gouvêa dos Santos, Margareth Spangler Andrade, José Mário Carneiro Vilela, Valbert Nascimento Cardoso, Gilson Andrade Ramaldes,
The present work describes the preparation, labeling, physicochemical characterization, and in vitro cytotoxic evaluation of long circulating pH-sensitive liposomes containing 159Gd-DTPA-BMA. These liposomes were successfully obtained and submitted to neutron irradiation for gadolinium labeling. Their size, distribution, and homogeneity were determined by photon correlation spectroscopy, while their zeta potential was determined by laser Doppler anemometry. The morphology and structural organization ...
Tópico(s): Radioactive element chemistry and processing
2011 - Elsevier BV | European Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Mônica Cristina Oliveira, André Luís Branco de Barros, Valbert Nascimento Cardoso, Gilson Andrade Ramaldes,
PEG-coated pH-sensitive and PEG-folate-coated pH-sensitive liposomes containing the Gd-DTPA-BMA complex were prepared and radiolabeled by neutron activation. The radiolabeled liposomes presented significant in vitro cytotoxic activity against Ehrlich tumor cells when compared with controls. The biodistribution profile of these liposomes and free 159Gd-DTPA-BMA were studied in mice bearing a previously-developed solid Ehrlich tumor. The results demonstrated an important uptake of the formulations ...
Tópico(s): Nanoparticle-Based Drug Delivery
2011 - Elsevier BV | European Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Valbert Nascimento Cardoso, André Luís Branco de Barros, Cristina Maria de Souza, Geovanni Dantas Cassali, Mônica Cristina Oliveira, Gilson Andrade Ramaldes,
In the present study, PEG-coated pH-sensitive and PEG-folate-coated pH-sensitive liposomes containing the 159Gd-DTPA-BMA were prepared and radiolabeled through neutron activation technique, aiming to study the in vivo antitumoral activity and toxicity on mice bearing a previously-developed solid Ehrlich tumor. The treatment efficacy was verified through tumoral volume increase and histomorphometry studies. The toxicity of formulations was investigated through animal weight variations, as well as ...
Tópico(s): Radiation Therapy and Dosimetry
2011 - Elsevier BV | European Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Guilherme Diniz Tavares, Cristiane M. Viana, José G.V.C. Araújo, Gilson Andrade Ramaldes, Wânia S. CARVALHO, Jorge L. Pesquero, José Mário Carneiro Vilela, Margareth Spangler Andrade, Mônica Cristina Oliveira,
Tópico(s): DNA and Nucleic Acid Chemistry
2006 - Elsevier BV | Chemical Physics Letters
Produção Nacional
Revisado por pares
Gilson Andrade Ramaldes, J. R. Deverre, J M Grognet, F. Puisieux, Elias Fattal,
The encapsulation efficiency of bovine serum albumin (BSA) within liposomes and its stability in physiological conditions were determined by a specific enzyme immunoassay (EIA) developed for this purpose. BSA was encapsulated within liposomes composed of soyabean phosphatidylcholine (PC), cholesterol (CH), phosphatidylglycerol (PG) (molar ratio 6:3:1) or distearoylphosphatidylcholine (DSPC), CH, PG, (molar ratio 6:3:1). Vesicles were prepared according to either the thin lipid film hydration or freeze-thawing ...
Tópico(s): Protein Interaction Studies and Fluorescence Analysis
1996 - Elsevier BV | International Journal of Pharmaceutics
Gilson Andrade Ramaldes, Elias Fattal, F. Puisieux, Michel Ollivon,
Tópico(s): Drug Transport and Resistance Mechanisms
1996 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Produção Nacional
Revisado por pares
Luciana Souto Ferreira, Gilson Andrade Ramaldes, E. A. Nunan, Lucas Antônio Miranda Ferreira,
Paromomycin (PA), a very hydrophilic antibiotic, has been tested as an alternative topical treatment against cutaneous leishmaniasis (CL). Although this treatment has shown promising results, it has not been successful in accelerating the recovery in most cases. This could be attributed to the low skin penetration of PA. Liposomal formulations usually provide sustained and enhanced drug levels in skin. The aim of this study was to prepare liposomal formulations containing PA and to investigate their ...
Tópico(s): Advancements in Transdermal Drug Delivery
2004 - Taylor & Francis | Drug Development and Industrial Pharmacy
Produção Nacional
Revisado por pares
Ana Luiza Chaves Maia, Carolina Henriques Cavalcante, Marina G.F. de Souza, Carolina A. Ferreira, Domenico Rubello, Sotirios Chondrogiannis, Valbert Nascimento Cardoso, Gilson Andrade Ramaldes, André Luís Branco de Barros, Daniel Crístian Ferreira Soares,
Hydroxyapatite is used as a drug-delivery system for bone therapy applications because of its biocompatibility, bioactivity, and osteoconductive properties. In addition, hydroxyapatite nanoparticles (HApN) might be used as a theranostic probe. The aim of this study was to prepare and characterize hydroxyapatite mesoporous nanoparticles, and radiolabel these nanoparticles with technetium-99m (Tc). Moreover, biodistribution studies were carried out in healthy mice.HApN were synthesized and characterized. ...
Tópico(s): Orthopedic Infections and Treatments
2016 - Lippincott Williams & Wilkins | Nuclear Medicine Communications
Produção Nacional
Revisado por pares
Ana Luiza Chaves Maia, Carolina A. Ferreira, André Luís Branco de Barros, Aline Teixeira Maciel e Silva, Gilson Andrade Ramaldes, Armando Silva‐Cunha, Diogo Coelho de Pádua Oliveira, Christian Fernandes, Daniel Crístian Ferreira Soares,
Despite advances in the development of new therapeutic agents and diagnostic imaging techniques, the 5-year survival of osteosarcoma, the most common type of bone cancer, remains practically unaltered for the last three decades at around 60%. Nanoparticle-based carriers have emerged as new class of drug delivery systems that could potentially overcome conventional chemotherapy limitations, by promoting a better drug biodistribution profile by allowing a preferential accumulation of the drug in the ...
Tópico(s): Nanoparticle-Based Drug Delivery
2017 - Taylor & Francis | Journal of drug targeting
Produção Nacional
Revisado por pares
Aline Teixeira Maciel e Silva, Ana Luiza Chaves Maia, Juliana de Oliveira Silva, André Luís Branco de Barros, Daniel Crístian Ferreira Soares, Mariana T. Q. de Magalhães, Ricardo José Alves, Gilson Andrade Ramaldes,
Carbohydrate receptors on liver represent attractive targets for receptor-mediated delivery of nanostructured therapeutics. In this study, two new cholesterol-based glycoconjugates derived from d-galactose and N-acetylglucosamine were synthesized and incorporated into liposomes. 99mTc-Cholesterol-DTPA complex was used for radiolabeling experiments in vivo with high radiochemical yields and stability. Biodistribution studies confirmed the targeting of galactosylated liposomes (GalL) to liver cells. ...
Tópico(s): Glycosylation and Glycoproteins Research
2018 - Elsevier BV | Carbohydrate Research
Mariana Cambão, Lígia Sousa, Miguel Santos, Sandra Mimoso, Sara Fernandes Correia, Dilermando Sobral,
... utentes inscritos na Unidade de Saúde Familiar de Ramalde, com idade ≥ 65 anos. Obteve-se uma amostragem ...
Tópico(s): Menopause: Health Impacts and Treatments
2019 - | Revista Portuguesa de Clínica Geral
Produção Nacional
Revisado por pares
Letícia Cristina Cidreira Boaro, Luiza Mello de Paiva Campos, Gustavo H.C. Varca, Tamiris Martins Ribeiro dos Santos, Pamela Adeline Marques, Mari Miura Sugii, Nathália Ramaldes SALDANHA, Karina Cogo, William Cunha Brandt, Roberto Ruggiero Braga, Duclerc Fernandes Parra,
The aim of this study was to develop a composite material with antibacterial activity using MMT loaded with clorhexidine (CHX). For that it was used a BisGMA/TEGDMA matrix and added low concentration of MMT/CHX. The aim was to evaluate the drug release capacity of MMT, and not to provide reinforcement.Six experimental composites were made with organic matrix of BisGMA/TEGDMA in equal proportions by weight. The composites received organophlizated montmorillonite with or without CHX. The concentrations ...
Tópico(s): Antimicrobial agents and applications
2019 - Elsevier BV | Dental Materials
Produção Nacional
Revisado por pares
Flávia Márcia Oliveira, Elisângela Martins dos Santos, Andréa C. Alves, Maria Aparecida Campana-Pereira, Gilson Andrade Ramaldes, Valbert Nascimento Cardoso, Valéria Ruiz‐de‐Souza, Célia Maria Ferreira Gontijo,
Previous work in this laboratory has demonstrated that ovalbumin coupled to palmitoyl residues (palmitoyl-Ova) does not induce oral tolerance. The present study sought to determine whether this coupling affects digestion, absorption and transfer of antigen. Ova and palmitoyl-Ova were shown to be digested differently in vitro by proteolytic enzymes and presented different tissue distribution kinetics after being labelled with (99m)technetium and orally administered to animals. Palmitoyl-Ova remained ...
Tópico(s): Advanced Drug Delivery Systems
2007 - Wiley | Scandinavian Journal of Immunology
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Andreza de Sousa Andrada, Gilson Andrade Ramaldes,
Today, cancer represents one of the main causes of death worldwide, making it a formidable challenge for all scientific areas that seek new therapeutic approaches for its cure. As a therapeutic approach, radiotherapy, widely used in treating various types of tumors, acts by not discriminating healthy cells from tumor cells. Seeking to minimize these effects, nanostructured carriers of radioisotopes have been studied with the aim of improving the specificity of action of ionizing radiation, delivering ...
Tópico(s): Medical Imaging Techniques and Applications
2014 - Taylor & Francis | Particulate Science And Technology
Produção Nacional
Revisado por pares
Sarah Simaan dos Santos, Luana Aparecida Ramaldes, Mônica Andrade Lima Gabbay, Regina S. Moisés, Sérgio Atala Dib,
Among the insulin resistance syndromes that lead to diabetes mellitus in young people, Rabson-Mendenhall syndrome (RMS; OMIM # 262190) is an autosomal recessive inherited disease caused by an insulin receptor mutation (INSR; 147,670). Due to the rarity and complexity of the disease, we have few therapeutic alternatives other than insulin with clinical studies with robust evidence. Some reports suggest the adjunct use of metreleptin, metformin, and pioglitazone with improved glycemic control, however, ...
Tópico(s): Protein Kinase Regulation and GTPase Signaling
2021 - Karger Publishers | Hormone Research in Paediatrics
Produção Nacional
Revisado por pares
F.P. Vieira, TAISSA LARA BARROS DE MESQUITA, Paulo Celso Pereira Lara, Gilson Andrade Ramaldes, Mark Anthony Beinner, Jos Bento B. Da Silva, Mônica Cristina Oliveira, Josianne Nicácio Silveira,
In this work, stability and the pH-sensitivity of pH-sensitive stealth liposomes containing cisplatin exposed to plasma medium and their subsequent responses to pH modifications were evaluated. A method to determine platin in mouse plasma by electrothermal atomic absorption spectroscopy (ET AAS) was developed and validated. At first, a comparative study of sample preparation treatments with basic, acidic, and acidic added with Triton X-100 as a modifier was done. The best treatment was obtained with ...
Tópico(s): RNA Interference and Gene Delivery
2013 - Elsevier BV | Journal of Pharmaceutical and Biomedical Analysis
Produção Nacional
Revisado por pares
Andréa C. Alves, Gilson Andrade Ramaldes, Mônica Cristina Oliveira, Valbert Nascimento Cardoso, T. A. Mota‐Santos, Ana Maria Caetano Faria, Célia Maria Ferreira Gontijo,
Oral administration of protein antigens, such as ovalbumin, may result in induction of either tolerance or immunization. To avoid oral tolerance, there are new strategies to protect the antigens from degradation within the gastrointestinal tract and to allow them to reach inductive immunological sites. One such strategy is the usage of liposomes. Different parameters may influence the stability of liposomes in the gastrointestinal tract. Herein, we studied the immunological consequences of oral ...
Tópico(s): Immune Cell Function and Interaction
2008 - Elsevier BV | Cellular Immunology
Produção Nacional
Revisado por pares
Daniel Crístian Ferreira Soares, Grasiely Faria de Sousa, André Luís Branco de Barros, Valbert Nascimento Cardoso, Mônica Cristina Oliveira, Gilson Andrade Ramaldes,
Theranostic nanosystems have attracted the attention of many scientists in worldwide due to its ability to accumulate and release selectively, in specific sites, compounds capable to generate images and to treat tumors, allowing simultaneously, the diagnose and treatment of cancer. Radioactive Gadolinium-159, a beta e gamma radiation emitter, has the properties to generate scintigraphic images and simultaneously, to conduct cancer cells to the death through high radiation doses. In this sense, the ...
Tópico(s): Radiopharmaceutical Chemistry and Applications
2015 - Elsevier BV | Journal of Drug Delivery Science and Technology
Produção Nacional
Revisado por pares
Ana Luiza Chaves Maia, Aline T. M. e Silva, Aina Liz Alves César, Cristiane dos Santos Giuberti, Fernanda Cristina Gontijo Evangelista, Janaína de Alcântara Lemos, Adriano de Paula Sabino, Ângelo Malachias, Christian Fernandes, André Luís Branco de Barros, Daniel Crístian Ferreira Soares, Gilson Andrade Ramaldes,
Gadodiamide (Gd-DTPA-BMA) is a gadolinium-based complex, used as a contrast agent in magnetic resonance imaging procedures. Recent studies revealed the capacity of this complex of inducing apoptosis in neoplastic cells. However, a great challenge for its use as an anticancer drug is low cellular internalization and drug delivery systems such as thermosensitive liposomes can be a strategy to overcome these limitations and increase anti-tumor efficacy. In this context, this study aimed to develop, ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2021 - Elsevier BV | Journal of Drug Delivery Science and Technology
Produção Nacional
Revisado por pares
Gilson Andrade Ramaldes, Maira Alves Pereira, Poliana Teixeira de Castro, Lauro Mello Vieira, Valbert Nascimento Cardoso,
O objetivo deste estudo foi marcar uma proteína utilizada como modelo, a soro albumina bovina (SAB), com 99mTecnécio (99mTc), encapsular a rroteína marcada (99mTc-SAB) em lipossomas e empregar este marcador para quantificar a 99m c-SAB capturada pelas placas de Peyer de camundongos Balb/c após administração oral. A 99mTc-SAB (taxa de marcação=94,9±2,4%; n=25) foi encapsulada em lipossomas multilamelar (MLV), unilamelar de pequeno tamanho (SUV) ou unilamelar de grande tamanho (LUV) compostos de fosfatidilcolina ...
Tópico(s): Protein purification and stability
2002 - Sociedade Brasileira de Ciência do Solo | Revista Brasileira de Ciência do Solo
Produção Nacional
Revisado por pares
Felipe Peixoto, P. Menezes Dias, Gilson Andrade Ramaldes, José Mário Carneiro Vilela, Margareth Spangler Andrade, Armando Silva‐Cunha,
Solid lipid nanoparticles (SLN) have generated increasing attention as an alternative carrier system, particularly for lipophilic drugs [1-2]. The system consists of lipid nanoparticles, which are solid at room temperature. The solid matrix offers the possibility to improve the stability against coalescence and the reduced mobility of incorporated drug molecules is a pre requisite for controlled drug release [3]. Dexamethasone acetate was used as a model drug because of its wide application in the ...
Tópico(s): Advancements in Transdermal Drug Delivery
2005 - Oxford University Press | Microscopy and Microanalysis
Produção Nacional
Revisado por pares
Andréa C. Alves, Cristiano Machado Gontijo, Mônica Cristina Oliveira, Simone Odília Antunes Fernandes, Flávia Márcia Oliveira, Valbert Nascimento Cardoso, Gilson Andrade Ramaldes,
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different ...
Tópico(s): RNA Interference and Gene Delivery
2005 - Instituto de Tecnologia do Paraná (Tecpar) | Brazilian Archives of Biology and Technology
Rita Rodrigues, José Ferrão Afonso,
... nave da Igreja Paroquial de S. Salvador de Ramalde e revestida por um tecto de quarenta e ...
Tópico(s): Historical Art and Architecture Studies
2013 - Catholic University of Portugal | Estudos de Conservação e Restauro