Meiqing Lei, Lingfeng Sun, Xian‐Sheng Luo, Xiaoyang Yang, Yu Feng, Xiaoxia Chen, Zhiming Wang,
... the predictive value of red blood cell lifespan (RBCLS), as determined by an automated CO breath test ... two common forms of microcytic hypochromic anemia (MHA).RBCLS measurements were conducted in 35 healthy controls (HCs) ... 55) with ELS TESTER that provides a direct RBCLS value read-out. RBCLS between IDA and Thal was compared and evaluated ... from the instrument.Compared with that in HCs, RBCLS in IDA and Thal groups was shortened; and median RBCLS was shorter in the Thal group than that ... versus 79 d, p < 0.001). The median RBCLS in IDA patients with chronic gastrointestinal (GI) bleeding ...
Tópico(s): Erythropoietin and Anemia Treatment
2019 - IOP Publishing | Journal of Breath Research
Jie Ma, Yanna Dou, Hanjie Zhang, Stephan Thijssen, Steven Williams, Viktoriya Kuntsevich, Georges Ouellet, Michelle MY Wong, Vanja Peršič, Anja Kruse, Laura Rosales, Yuedong Wang, Nathan W. Levin, Peter Kotanko,
... factors, with decreased red blood cell life span (RBCLS) being a significant contributor. Although the impact of reduced RBCLS on anemia is recognized, it is still a ... this study was to investigate the relationship between RBCLS and inflammatory biomarkers in chronic HD patients. <b><i>Design, Setting, Participants, and Measurements:</i></b> RBCLS was calculated from alveolar carbon monoxide concentrations measured ... baseline and during follow-up. The associations between RBCLS and inflammatory biomarkers were evaluated using linear mixed ...
Tópico(s): Iron Metabolism and Disorders
2017 - Karger Publishers | Blood Purification
Jidnyasa Joshi, Oliver Mueller‐Cajar, Yi-Chin Candace Tsai, F. Ulrich Hartl, Manajit Hayer‐Hartl,
... is a cylindrical complex composed of eight large (RbcL) subunits that are capped by four small subunits ( ... considerable biotechnological interest. Folding of the green-type RbcL subunits in cyanobacteria is mediated by the GroEL/ ... RAF1. Here, we show that the red-type RbcL subunits in the proteobacterium Rhodobacter sphaeroides also fold ... protrudes into the central solvent channel of the RbcL core complex. The two β-barrels stabilize the complex through multiple interactions with the RbcL subunits. A chimeric green-type RbcS carrying the ...
Tópico(s): Light effects on plants
2014 - Elsevier BV | Journal of Biological Chemistry
Lior Doron, Na’ama Segal, Hadas Gibori, Michal Shapira,
... of DnaJ. BSD 2 co‐migrated with the rbcL transcript on heavy polysomes, and both BSD 2 and rbcL m RNA shifted to the lighter fractions under ... repress the translation of the Rubisco large subunit (RbcL). This profile of co‐migration supports the possibility ... is required for the de novo synthesis of RbcL. Furthermore, BSD 2 co‐migrated with the rbcL transcript in a C. reinhardtii premature‐termination mutant that encodes the first 60 amino acids of RbcL. In both strains, BSD 2 shared its migration profile with the rbcL transcript but not with psbA m RNA . The ...
Tópico(s): Protein Structure and Dynamics
2014 - Wiley | The Plant Journal
Shaun M. Bowman, Minesh Patel, Pradeep Yerramsetty, Christopher M Mure, Amy M. Zielinski, Jeremy A. Bruenn, James O. Berry,
... and function. In mature C4 leaves, the plastidic rbcL gene, encoding the large subunit of the primary ... cells. Numerous studies have demonstrated that BS-specific rbcL gene expression is regulated predominantly at post-transcriptional ... of regulatory factors associated with C4 patterns of rbcL gene expression has been an elusive goal for ... based on its specific binding to plastid-encoded rbcL mRNA in vitro. Co-localized with LSU to ... and Arabidopsis (C3) reveals its tight association with rbcL gene expression in both plants. Reduced RLSB expression ( ...
Tópico(s): Lipid metabolism and biosynthesis
2013 - BioMed Central | BMC Plant Biology
... with declines in those of the large subunit (RBCL) being relatively slower. The amount of synthesized RBCL per unit level of RBCL mRNA and polysome loading of RBCL mRNA declined in senescent leaves, whereas such phenomena ... RBCS. These results suggested that gene expression of RBCL is downregulated at the level of its translation when a balance between RBCL and RBCS expression is disturbed by leaf senescence. ... that RBCS protein is a positive regulator for RBCL mRNA level in expanding rice leaves, as judged ...
Tópico(s): Plant Stress Responses and Tolerance
2013 - Oxford University Press | Journal of Experimental Botany
Yanna Dou, Anja Kruse, Peter Kotanko, Herman Rosen, Nathan W. Levin, Stephan Thijssen,
... than a binary outcome, and RBC life span (RBCLS) is a basic component of the process.2. ... 254Crossref PubMed Scopus (37) Google Scholar Reduction in RBCLS will necessarily entail a reduction in Hgb unless ... regardless of increases in EPO dose, a short RBCLS becomes the limiting factor in Hgb increase, giving ... impression of resistance to EPO. We have determined RBCLS repeatedly in 44 hemodialysis (HD) patients by carbon ... 399PubMed Google Scholar and saw an average baseline RBCLS of 70±23 days, which is in keeping ...
Tópico(s): Hemoglobinopathies and Related Disorders
2012 - Elsevier BV | Kidney International
Mike Thiv, Timotheüs van der Niet, Frank Rutschmann, Mats Thulin, Thomas Brune, H. P. Linder,
... analysis of the ITS, matK, and the atpB-rbcL spacer (Table 2), rooted with the the closely ... Collector No. Origin voucher ITS1 ITS2 matK atpB-rbcL spacer rbcL Cneoridium dumosum Hook.f. Thibault et Denslow s. ... and two taxa with reliable fossils for the rbcL analysis. Thamnosma was represented by six North American ... on only half of the gene length of rbcL (790–813 bp). Laboratory work The molecular work ... absence of paralogous copies of ITS. The atpB-rbcL intergenic spacer: atpB-F2 5′-GAAGTAGTAGGATTGATTCTC-3′, atpB- ...
Tópico(s): Botanical Studies and Applications
2010 - Wiley | American Journal of Botany
... Google Scholar) for the products of the L (rbcL) and S (rbcS) genes allowing their assembly into ... other species, the rbcX gene is situated between rbcL and rbcS (Larimer and Soper, 1993Larimer F.W. ... newly translated Rubisco L polypeptides. Soon after folded RbcL emerges, RbcX maximizes the correct assembly of RbcL octamers; otherwise, RbcL has a tendency to misassemble or produce unproductive ... is essential and facilitates the formation of soluble RbcL subunits, whereas the polar surface region ensures that ...
Tópico(s): Algal biology and biofuel production
2007 - Cell Press | Cell
Spencer M. Whitney, Robert E. Sharwood,
... and S. For the Rubisco L subunit gene (rbcL) located in the plastid genome (plastome), genetic manipulation ... highlighted by the transplastomic replacement of the tobacco rbcL with sunflower rbcL that produced tobacco-sunflower transformants whose hybrid sunflower ... higher plant Rubiscos, is coded by a single rbcL-rbcS operon and can be functionally expressed in ... type PCC6301 Rubisco Genes—The Synechococcus PCC6301 Rubisco rbcL-S operon was amplified from pSH1 (28Andrews T. ... 3′, the NdeI site is underlined, and the rbcL initiator codon is in bold) and 3′SacISynS ( ...
Tópico(s): Algal biology and biofuel production
2006 - Elsevier BV | Journal of Biological Chemistry
Alyssa Carré‐Mlouka, Annick Méjean, Philippe Quillardet, Hiroki Ashida, Y. SAITO, Akiho Yokota, Isabelle Callebaut, Agnieszka Sekowska, Elke Dittmann, Christiane Bouchier, Nicole Tandeau de Marsac,
... PCC 7806 that forms water blooms worldwide. The rbcL(I) gene belongs to the form I subfamily ... aeruginosa PCC 7806 cells, the expression of both rbcL(I) and rbcL(IV) is sulfur-dependent. The purified recombinant RbcL(IV) overexpressed in Escherichia coli cells did not ... 3-diketo-5-methylthiopentyl-1-phosphate, and the rbcL(IV) gene rescued a Bacillus subtilis MtnW-deficient mutant. Therefore, the Microcystis RbcL(IV) protein functions both in vitro and in ... pathway. Despite variations in the amino acid sequences, RbcL(IV) shares structural similarities with all members of ...
Tópico(s): Algal biology and biofuel production
2006 - Elsevier BV | Journal of Biological Chemistry
Ido Yosef, Vered Irihimovitch, Joel A. Knopf, Idan Cohen, Irit Orr-Dahan, Eyal Nahum, Chen Keasar, Michal Shapira,
... protein interactions with the 5′-untranslated region of rbcL. This RNA region specifically binds a group of ... protein interactions with the 5′-untranslated region of rbcL. This RNA region specifically binds a group of ... eight large subunits (LSU) encoded by the chloroplast rbcL gene and eight small subunits encoded by the ... RNA-binding proteins affected their interaction with the rbcL leader. We show that the interaction between RNA-binding proteins and the rbcL leader is interrupted by oxidative stress and that ...
Tópico(s): Photoreceptor and optogenetics research
2004 - Elsevier BV | Journal of Biological Chemistry
Yuchun Du, Srinivasa R. Peddi, Robert J. Spreitzer,
... 20 Å away from the active site. The rbcL gene is one of the most sequenced genes ... chloroplasts of eukaryotes. There are more than 2000 rbcL entries in the National Center for Biotechnology Information Entrez Proteins data base. Because rbcL encodes the catalytic large subunit of the ratelimiting ... 55-kDa large subunits (coded by the chloroplast rbcL gene) and eight ∼16-kDa small subunits (coded ... acetate as an alternative carbon source, and its rbcL and rbcS genes can both be eliminated or ... chloroplast transformation. Mutant 18-7G results from an rbcL UAG nonsense mutation that terminates large subunit translation ...
Tópico(s): Porphyrin Metabolism and Disorders
2003 - Elsevier BV | Journal of Biological Chemistry
Shen Luo, H. Ishida, Amane Makino, Tadahiko Mae,
Previous work has demonstrated that the large subunit (rbcL) of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo) ... 5226). In this study, we found that the rbcL could also be cleaved into several other fragments ... but near the bound metal in the adjacent rbcL. All of these residues are at or very ... the fragmentation. These results strongly suggest that the rbcL is cleaved by reactive oxygen species generated at ... Previous work has demonstrated that the large subunit (rbcL) of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo) ...
Tópico(s): Porphyrin Metabolism and Disorders
2002 - Elsevier BV | Journal of Biological Chemistry
Jong Wan Kim, Jeong Koo Park, Byung Hoon Kim, Jaeseon Lee, Woong Seop Sim,
We previously reported that the rbcL transcript level was significantly increased by light signal [Lee and Sim (1995)]. To investigate whether or not the accumulation of rbcL mRNA by light signal is caused by an increase of transcription activity of the rbcL gene, run-on transcription assays were performed. The results indicated that the accumulation of rbcL mRNA is due to an increase in the transcriptional activity of the rbcL gene by light. An electrophoretic mobility shift assay ( ... the specific binding proteins that interact with the rbcL promoter region. As a result of EMSA, a ...
Tópico(s): Soybean genetics and cultivation
2002 - Springer Science+Business Media | Molecules and Cells
Hisayoshi Nozaki, Manabu Takahara, Atsushi Nakazawa, Yoko Kita, Takashi Yamada, Hiroyoshi Takano, Shigeyuki Kawano, Masahiro Kato,
... position identical to that of the chloroplast-encoded rbcL coding region (rbcL-462 introns) and their ORFs from 13 strains ... and a related unicellular green alga, Vitreochlamys. The rbcL-462 introns contained an intact or degenerate ORF of various sizes except for the Gonium multicoccum rbcL-462 intron. Partial amino acid sequences of some rbcL-462 intron ORFs exhibited possible homology to the ... amino acid terminal domain. The distribution of the rbcL-462 introns is sporadic in the phylogenetic trees ...
Tópico(s): Genomics and Phylogenetic Studies
2002 - Elsevier BV | Molecular Phylogenetics and Evolution
... probes specific for the coding region of the rbcL and psbA mRNAs revealed that over-expression of ... led to a strong decrease in correctly processed rbcL and psbA mRNAs. Interestingly, an increased accumulation of ... Despite the weaker appearance of the rather heterogeneous rbcL specific signals in PNP− plants in northern analyses, additional quantification of total rbcL and psbA mRNA amounts (mature plus unprocessed mRNA) ... In contrast, the stability of mature psbA and rbcL mRNAs appears to remain unaffected by PNPase over- ...
Tópico(s): RNA and protein synthesis mechanisms
2002 - Springer Nature | The EMBO Journal
... ions.psbA mRNA (D1 protein of photosystem II),rbcL mRNA (large subunit of ribulose-1,5-bisphosphate ... ions.psbA mRNA (D1 protein of photosystem II),rbcL mRNA (large subunit of ribulose-1,5-bisphosphate ... transcripts. cis-Regulatory elements for the degradation of rbcL mRNA in Chlamydomonashave also been analyzed (15Salvador M. ... polysome association and mRNA stability was suggested for rbcL mRNA from studies of nuclear mutants in maize. ... abnormally few ribosomes showed that the level of rbcL mRNA was reduced 4-fold, indicating that the ...
Tópico(s): Plant Micronutrient Interactions and Effects
2000 - Elsevier BV | Journal of Biological Chemistry
... ribosomal protein S1 (Figure 1B). The analysis of rbcL gene expression, a gene that is under the ... 1991) of Wt and Sp spinach plantlets (the rbcL Wt and Sp cDNAs have been inversely loaded ... the same primers. Primers were 5′-AGATATCAGTATCTAGGG-3′ (rbcL), 5′-GCATCTTCCTCTCCAGG-3′ (clpP) and 5′-TTCATAGTTGCATTACT-3′ (rrn). Transcription start sites are −180 (rbcL), −53 (clpP) and PC (rrn). The −64 transcript (rbcL) is a processing intermediate. Download figure Download PowerPoint ... fractions contain mainly PEP and recognize specifically the rbcL promoter but not the rrn promoter (Iratni et ...
Tópico(s): Genomics and Phylogenetic Studies
2000 - Springer Nature | The EMBO Journal
Joaquín Moreno, Robert J. Spreitzer,
... 55-kDa large subunits (encoded by the chloroplast rbcL gene) and eight 15-kDa small subunits (encoded ... 6297Crossref PubMed Google Scholar). It results from an rbcL nonsense mutation that changes large-subunit Trp-66 ... fragment (bases −175 to 2776), containing the entire rbcL gene (bases 1–1428) (32Dron M. Rahire M. ... Google Scholar). To produce the C172S mutation, the rbcL gene sequence TGT (bases 514–516) was changed ... Genosys Biotechnologies, Inc.). The plasmid containing the C172S rbcL mutant gene was named pLS-C172S. Chloroplast transformation ...
Tópico(s): Microbial bioremediation and biosurfactants
1999 - Elsevier BV | Journal of Biological Chemistry
Andrea D. Wolfe, Claude W. dePamphilis,
The photosynthetic gene rbcL has been lost or dramatically altered in some lineages of nonphotosynthetic parasitic plants, but the dynamics of these events following loss of photosynthesis and whether rbcL has sustained functionally significant changes in photosynthetic parasitic plants are unknown. To assess the changes to rbcL associated with the loss of functional constraints for ... holoparasites"). With the exception of Conopholis (Orobanchaceae), the rbcL locus is present in all parasitic plants of ...
Tópico(s): Photosynthetic Processes and Mechanisms
1998 - Oxford University Press | Molecular Biology and Evolution
Qiu‐Yun Xiang, Pamela S. Soltis, Pamela S. Soltis,
... with those based on previous phylogenetic analysis of rbcL sequences. Analysis of a combined matK and rbcL sequence data set (a total of 2652 bp [ ... morphology and gynoecial vasculature. Comparisons of matK and rbcL sequences based on one of the most parsimonious rbcL‐matK trees indicate that matK has a much ... 66.9% in matK vs. 55.8% in rbcL ) and a lower transition:transversion ratio (1.23 in matK vs. 2.21 in rbcL ). The total number of nucleotide substitutions per site for matK is 2.1 times that of rbcL in Cornales. These findings are similar to recent ...
Tópico(s): Mycorrhizal Fungi and Plant Interactions
1998 - Wiley | American Journal of Botany
SL Pichard, Marc E. Frischer, JH Paul,
... have measured levels of the RuBPCase large subunit (rbcL) mRNA and DNA, in combination with rates of ... and the southeastern Gulf of Mexico.We measured rbcL mRNA, rbcL DNA, and the ratio of rbcL mRNA to rbcL DNA by extracting RNA and DNA and probing the extracts with the Synechococcus PCC 6301 rbcL gene as a probe.Additionally, rbcL mRNA was amplified from certain samples using a ... a transect from Tampa Bay seaward, levels of rbcL mRNA decreased 3to 8-fold from the estuarine ... offshore vertical profile, the subsurface maximum in the rbcL mRNA/DNA ratio coincided with the 60 m ...
Tópico(s): Microbial Community Ecology and Physiology
1993 - Inter-Research | Marine Ecology Progress Series
Yanbo Huang, Claudiane Ouellet‐Plamondon, Steven J. Thomson, Krishna N. Reddy,
Abstract: Rubidium chloride (RbCl) was used as a tracer tank-mixed with active ingredients to profile downwind deposition of aerially applied crop protection ... at each sampling station to collect sprayed solution. RbCl tracer was used to assess downwind drift of ... sampling station location had a significant effect on RbCl concentration. However, application release altitude was not significant to the change of RbCl. Another practical application in the same aerial application ... the off-target drift of aerially applied glyphosate. RbCl concentrations measured from Mylar sheets were correlated with ...
Tópico(s): Plant Surface Properties and Treatments
2017 - Chinese Society of Agricultural Engineering | International journal of agricultural and biological engineering
Dandan Gao, Dongdong Li, Wu Li,
Solubility of RbCl(cr) and CsCl(cr) in pure water were determined at subzero temperatures. The solubility of RbCl(cr) and CsCl(cr) decreased with temperature increment. Specific heat capacity of RbCl(aq) and CsCl(aq) solutions were determined at ... to near saturation. The specific heat capacity of RbCl(aq) and CsCl(aq) solutions decreased with concentration ... of CsCl(aq) is smaller than that of RbCl(aq). A temperature dependent thermodynamic model based on ... activity, thermal and solid-liquid equilibria properties of RbCl + H2O and CsCl + H2O systems. The obtained models ...
Tópico(s): Calcium Carbonate Crystallization and Inhibition
2016 - Elsevier BV | The Journal of Chemical Thermodynamics
Nastaran Kordjazy, Arya Haj‐Mirzaian, Shayan Amiri, Sattar Ostadhadi, Mehdi Kordjazy, Mohammad Sharifzadeh, Ahmad Reza Dehpour,
... We examined the antidepressant activity of rubidium chloride (RbCl) in male mice and the possible interference of ... used to evaluate the antidepressant-like effect of RbCl. These drugs were used in this study: NG- ... serum and hippocampus nitrite level after different treatments. RbCl (30 mg/kg), when administered 60 min before ... kg), co-administered with the effective dose of RbCl (30 mg/kg), reversed the anti-immobility effect of RbCl, while 7-NI (25 mg/kg) could not prevent the diminishing effect of RbCl on immobility time. Moreover, co-administration of non- ...
Tópico(s): Neuroscience and Neuropharmacology Research
2015 - Elsevier BV | European Journal of Pharmacology
... and eight large subunits coded for by the rbcL gene in the plastome. For synthesis of the ... suppression of the RBCS gene. Total RBCS and rbcL messenger RNA (mRNA) levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants, although ... amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with ... major chloroplast-encoded photosynthetic genes. The level of rbcL mRNA, in turn, was tightly correlated with the amount of RbcL synthesized, the molar ratio of RBCS synthesis to ...
Tópico(s): Plant Reproductive Biology
2012 - Oxford University Press | PLANT PHYSIOLOGY
Michel Goldschmidt‐Clermont, Michèle Rahire, Jean‐David Rochaix,
... mutants affected in 3′ maturation of the chloroplast rbcL mRNA in Chlamydomonas reinhardtii . We inserted a spectinomycin resistance cassette, 5′ atpA::aadA:: 3′ rbcL , in a peripheral domain of tscA , the gene ... which was due to processing at the 3′ rbcL element: the chimeric tscA RNA was truncated and ... of the second inverted repeat in the 3′ rbcL (IR2), and one deleted a larger region including ... the 3′ untranslated region (UTR) of the native rbcL gene, the rbcL mRNA accumulated to normal levels, but in strong ...
Tópico(s): Genomics and Phylogenetic Studies
2007 - Wiley | The Plant Journal
Dennis J. McCormac, Hanz Litz, Jianxin Wang, Paul Gollnick, James O. Berry,
... amaranth, a C(4) dicotyledonous plant, the plastid rbcL gene (encoding the large subunit of ribulose-1, ... mediated development. To identify post-transcriptional regulators of rbcL expression, three types of analyses (polysome heel printing, ... that multiple proteins interact with 5' regions of rbcL mRNA in light-grown, but not etiolated, amaranth ... UV cross-linking, was highly specific for the rbcL 5' RNA. Binding of p47 occurred only with RNAs corresponding to mature processed rbcL transcripts (5'-untranslated region (UTR) terminating at -66); ...
Tópico(s): Photoreceptor and optogenetics research
2001 - Elsevier BV | Journal of Biological Chemistry
JH Paul, Albin Alfreider, JB Kang, R.A. Stokes, David A. Griffin, Lisa Campbell, Erla Björk Örnólfsdóttir,
... 8 (2000) - doi:10.3354/meps198001 Form IA rbcL transcripts associated with a low salinity/high chlorophyll ... Ribulose-1,5-diphosphate carboxylase large subunit gene (rbcL) transcriptional activity. One sampling site (Stn 4) was ... 1.1 ng l-1) of Form IA rbcL mRNA in the surface water as determined by probing with a 1.1 kb Synechococcus WH7803 rbcL probe. Form IA rbcLs have been found to occur mainly in chemosynthetic ... the surface waters. The amount of Form IA rbcL was only about 3% of the concentration found ...
Tópico(s): Coral and Marine Ecosystems Studies
2000 - Inter-Research | Marine Ecology Progress Series