Stephan Nickell, R. Hegerl, Wolfgang Baumeister, Reinhard Rachel,
The hyperthermophilic archaeon Pyrodictium grows in the form of a macroscopically visible network. It consists of cells entrapped in an extracellular matrix of hollow tubules, the "cannulae." Here, we present the three-dimensional structure of a single cell in conjunction with two extracellular cannulae, as determined by cryo-electron microscopy. To achieve this, the information from two independent tilt series of the same specimen was combined, with the specimen rotated in the second series. In ...
Tópico(s): Iron oxide chemistry and applications
2003 - Elsevier BV | Journal of Structural Biology
Mikhail Kudryashev, Marek Cyrklaff, Björn Alex, Leandro Lemgruber, Wolfgang Baumeister, Reinhard Wallich, Friedrich Frischknecht,
Some Borrelia species are the causative agents of tick-borne Lyme disease responsible for different disabilities depending on species and hosts. Borrelia are highly motile bacterial cells, and light microscopy shows that these spirochetes can associate with each other during movement. Using cryo-electron tomography, we observed closely associated Borrelia cells. Some of these showed a single outer membrane surrounding two longitudinally arranged cytoplasmic cylinders. We also observed fusion of ...
Tópico(s): Vector-Borne Animal Diseases
2011 - Wiley | Cellular Microbiology
Johannes Hoffmann, Jan P. Fertmann, R diger G. H. Baumeister, Reinhard Putz, Andreas Frick,
Lipectomy is a standard procedure in plastic surgery. Until now, however, there was no definite information about the influence of different liposuction techniques (tumescent versus dry liposuction) on the integrity of lymph collectors during this procedure. To study the effect of these liposuction techniques on the incidence of lymph vessel injury, postmortem lymphatic preparations were done in nine human cadavers (18 lower extremities). Conventional liposuction with a blunt 4-mm cannula in the dry ...
Tópico(s): Breast Implant and Reconstruction
2004 - Lippincott Williams & Wilkins | Plastic & Reconstructive Surgery
Andreas Frick, Johannes Hoffmann, R. G. H. Baumeister, Reinhard Putz,
Liposuction is a standard procedure in plastic surgery. Especially, lipectomy with suction of the lower extremities has been of greater interest in recent years. Until now, however, there was no definite information about the integrity of epifascial lymph collectors during this procedure. To study the effect of liposuction devices on lymph vessel injury, postmortem lymphatic preparations were done in five human cadavers (10 lower extremities). Conventional liposuction with a blunt 4-mm cannula and ...
Tópico(s): Dupuytren's Contracture and Treatments
1999 - Lippincott Williams & Wilkins | Plastic & Reconstructive Surgery
Andreas Frick, Johannes Hoffmann, R. G. H. Baumeister, Reinhard Putz,
... Andreas M.D.; Hoffmann, Johannes N. M.D.; Baumeister, Rüdiger G. H. M.D.; Putz, Reinhard M.D. Author Information
Tópico(s): Skin Diseases and Diabetes
1999 - Lippincott Williams & Wilkins | Plastic & Reconstructive Surgery
... dem Zufall überlassen bleiben, noch zwangsweise herbeigeführt werden'. Reinhard Baumeister, Stadt-Erweiterungen in technischer, baupolizeilicher und wirtschaftlicher Beziehung ( ...
Tópico(s): Historical Architecture and Urbanism
2012 - Taylor & Francis | Planning Perspectives
Sascha Eulert, Martina Niewerth, Jana Hörstermann, Claudia Sengler, Daniel Windschall, Tilmann Kallinich, J. Grulich‐Henn, Frank Weller‐Heinemann, Ivan Foeldvari, Sandra Hansmann, Harald Baumeister, Reinhard W. Holl, Doris Staab, Kirsten Minden,
... BMS, Sanofi, MEDAC, Sandra Hansmann: None declared, Harald Baumeister: None declared, Reinhard Holl: None declared, Doris Staab: None declared, Kirsten ...
Tópico(s): Autoimmune and Inflammatory Disorders Research
2021 - BMJ | Annals of the Rheumatic Diseases
Petra Warschburger, Ann-Christin Petersen, Roman E. von Rezori, Friederike Buchallik, Harald Baumeister, Reinhard W. Holl, Kirsten Minden, Annabel Sandra Mueller‐Stierlin, Christina Reinauer, Doris Staab,
Abstract Background Relatively little is known about protective factors and the emergence and maintenance of positive outcomes in the field of adolescents with chronic conditions. Therefore, the primary aim of the study is to acquire a deeper understanding of the dynamic process of resilience factors, coping strategies and psychosocial adjustment of adolescents living with chronic conditions. Methods/design We plan to consecutively recruit N = 450 adolescents (12–21 years) from three German patient ...
Tópico(s): Family Support in Illness
2021 - BioMed Central | BMC Pediatrics
Enxian Shi, Zhengquan Wu, Birnur Sinem Karaoglan, Sabina Schwenk‐Zieger, Gisela Kranz, Nilofer Abdul Razak, Christoph A. Reichel, Martin Canis, Philipp Baumeister, Reinhard Zeidler, Olivier Gires,
Epithelial-to-mesenchymal transition (EMT) of malignant cells is a driving force of disease progression in human papillomavirus-negative (HPV-negative) head and neck squamous cell carcinomas (HNSCC). Sustained hyper-activation of epidermal growth factor receptor (EGFR) induces an invasion-promoting subtype of EMT (EGFR-EMT) characterized by a gene signature ("'EGFR-EMT_Signature'") comprising 5´-ectonucleotidase CD73. Generally, CD73 promotes immune evasion via adenosine (ADO) formation and associates with ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2023 - BioMed Central | Journal of Biomedical Science
Wolfgang Baumeister, Franck Hugues Karrenberg, Reinhard Rachel, Andreas Engel, B. ten Heggeler, W. O. Saxton,
The so‐called HPI layer, representing the major cell envelope protein of the radiotolerant bacterium Micrococcus radiodurans , is a hexagonally patterned macromolecular monolayer; it is intimately associated with the outer membraneous layer (‘backing layer’) of the bacterium and covers its surface completely. By virtue of its quite unusual resistance to chemical perturbants it can easily be isolated, e.g. by detergent extraction of whole cells. A detailed description of the morphology of the HPI layer ...
Tópico(s): Enzyme Structure and Function
1982 - Wiley | European Journal of Biochemistry
Mikhail Kudryashev, Marek Cyrklaff, Wolfgang Baumeister, Markus M. Simon, Reinhard Wallich, Friedrich Frischknecht,
Spirochetes of the Borrelia burgdorferi sensu lato group, the causative agents of Lyme borreliosis, exhibit a complex biology evolved in its zoonotic cycle. Cryo-electron tomography was used to investigate structural features of three species, B. burgdorferi, B. garinii and B. afzelii, known to cause different clinical manifestations in humans. All three organisms revealed an overall similar architecture and showed different numbers of periplasmic flagellar filaments, polar periplasmic void regions, ...
Tópico(s): Bartonella species infections research
2009 - Wiley | Molecular Microbiology
Frederike Lunkenheimer, Alexander J. Eckert, Dörte Hilgard, Daniel Köth, Bernhard Kulzer, Ursula Lück, Blanca Lüdecke, A Müller, Harald Baumeister, Reinhard W. Holl,
Mental comorbidities in patients with type 1 diabetes mellitus (T1D) are common, and can have a negative impact on acute blood glucose levels and long-term metabolic control. Information on the association of T1D and comorbid posttraumatic stress disorder (PTSD) with diabetes-related outcomes is limited. The aim was to examine the associations between a clinical diagnosis of PTSD and diabetes-related outcomes in patients with T1D. Patients with T1D and comorbid documented PTSD from the DPV database ( ...
Tópico(s): Childhood Cancer Survivors' Quality of Life
2023 - Nature Portfolio | Scientific Reports
René G. Feichtinger, Bettina E. Mucha, Holger Hengel, Zakaria Orfi, Christine Makowski, Junio Dort, Guy D’Anjou, Thi Tuyet Mai Nguyen, Rebecca Buchert, Hendrik Juenger, Peter Freisinger, Sarah Baumeister, Benedikt Schoser, Uwe Ahting, Reinhard Keimer, Cam‐Tu Émilie Nguyen, Paul Fabre, Julie Gauthier, Marguerite Miguet, Fátima Lopes, Afnan AlHakeem, Amal Alhashem, Brahim Tabarki, Krishna Kumar Kandaswamy, Peter Bauer, Peter Steinbacher, Holger Prokisch, Marc Sturm, Tim M. Strom, Benjamin Ellezam, Johannes A. Mayr, Lüdger Schöls, Jacques L. Michaud, Philippe M. Campeau, Tobias B. Haack, Nicolas A. Dumont,
PurposeSkeletal muscle growth and regeneration rely on muscle stem cells, called satellite cells. Specific transcription factors, particularly PAX7, are key regulators of the function of these cells. Knockout of this factor in mice leads to poor postnatal survival; however, the consequences of a lack of PAX7 in humans have not been established.MethodsHere, we study five individuals with myopathy of variable severity from four unrelated consanguineous couples. Exome sequencing identified pathogenic ...
Tópico(s): Tissue Engineering and Regenerative Medicine
2019 - Elsevier BV | Genetics in Medicine
Thomas Binz, Juan Blasi, Shinji Yamasaki, A. Baumeister, E. Link, Thomas C. Südhof, Reinhard Jahn, Heiner Niemann,
Clostridial neurotoxins, tetanus toxin (TeTx) and the seven related but serologically distinct botulinal neurotoxins (BoNT/A to BoNT/G), are potent inhibitors of synaptic vesicle exocytosis in nerve endings. Recently it was reported that the light chains of clostridial neurotoxins act as zinc-dependent metalloproteases which specifically cleave synaptic target proteins such as synaptobrevin/VAMPs, HPC-1/syntaxin (BoNT/C1), and SNAP-25 (BoNT/A). We show here that BoNT/E, like BoNT/A, cleaves SNAP-25, ...
Tópico(s): Neurological disorders and treatments
1994 - Elsevier BV | Journal of Biological Chemistry
Mikhail Kudryashev, Marek Cyrklaff, Reinhard Wallich, Wolfgang Baumeister, Friedrich Frischknecht,
Bacteria can be propelled in liquids by flagellar filaments that are attached to and moved by flagellar motors. These motors are rotary nanomachines that use the electrochemical potential from ion gradients. The motor can spin in both directions with specific proteins regulating the direction in response to chemotactic stimuli. Here we investigated the structure of flagellar motors of Borrelia spirochetes, the causative agents of Lyme disease in humans. We revealed the structure of the motor complex ...
Tópico(s): Escherichia coli research studies
2009 - Elsevier BV | Journal of Structural Biology
Katharina Köstner, Agnes Geirhos, Ramona Ranz, Angela Galler, Hanna Schöttler, Daniela Klose, L Feldhahn, Monika Flury, Katja Schaaf, Paul‐Martin Holterhus, Thomas Meißner, Petra Warschburger, Kirsten Minden, Svenja Temming, Annabel Sandra Mueller‐Stierlin, Harald Baumeister, Reinhard W. Holl,
Zusammenfassung Die interdisziplinäre Forschungskooperation COACH-Konsortium (Chronic Conditions in Adolescents – Implementation and Evaluation of Patient-centered Collaborative Healthcare) untersucht die psychosoziale Situation von Jugendlichen und jungen Erwachsenen mit chronischen körperlichen Erkrankungen. Zur Untersuchung der psychischen Komorbidität wurden bisher 1.023 Patienten mit Diabetes mellitus Typ 1 im Alter von 12–21 Jahren bei Routinevorstellungen in der Klinik bzw. Ambulanz mittels ...
Tópico(s): Bipolar Disorder and Treatment
2021 - Georg Thieme Verlag | Diabetologie und Stoffwechsel
E. Link, Juan Blasi, Edwin R. Chapman, Lambert Edelmann, A. Baumeister, Thomas Binz, Shinji Yamasaki, Heiner Niemann, Reinhard Jahn,
Tópico(s): Genetic Neurodegenerative Diseases
1994 - Academic Press | Advances in second messenger and phosphoprotein research
Wolfgang Baumeister, R. Guckenberger, Harald Engelhardt, Christopher L. Woodcock,
... Electron Microscopy of Biological Macromolecules WOLFGANG BAUMEISTER, WOLFGANG BAUMEISTER Max-Planck-Institut für Biochemie, D-8033 Martinsried, Federal Republic of GermanySearch for more papers by this authorREINHARD GUCKENBERGER, REINHARD GUCKENBERGER Max-Planck-Institut für Biochemie, D-8033 ... more papers by this author WOLFGANG BAUMEISTER, WOLFGANG BAUMEISTER Max-Planck-Institut für Biochemie, D-8033 Martinsried, Federal Republic of GermanySearch for more papers by this authorREINHARD GUCKENBERGER, REINHARD GUCKENBERGER Max-Planck-Institut für Biochemie, D-8033 ...
Tópico(s): Ion-surface interactions and analysis
1986 - Wiley | Annals of the New York Academy of Sciences
Reinhard Hatko, Joachim Baumeister, Volker Belli, Frank Puppe,
In this paper, we introduce the formal representation language DiaFlux, that is simple and easy to use on the one hand. On the other hand it allows for the definition of Computer-Interpretable Guidelines (CIGs), that can solve valuable tasks being executed in the clinical context. Further, we describe a wiki-driven development process using the stepwise formalization and allowing for almost self-acquisition by the domain specialists. The applicability of the approach is demonstrated by a protocol ...
Tópico(s): Electronic Health Records Systems
2012 - Springer Science+Business Media | Lecture notes in computer science
Katharina Förtsch, Rabea Viermann, Christina Reinauer, Harald Baumeister, Petra Warschburger, Reinhard W. Holl, Matthias Domhardt, Lisa Krassuski, Anna Lena Platzbecker, Hannah Kammering, Jennifer Cruz, Kirsten Minden, Doris Staab, Thomas Meißner,
The impact of the COVID-19 pandemic on the mental health of adolescents is of great concern, especially in the vulnerable group of adolescents with chronic medical conditions. The aim of this study was to examine this impact on the mental health of adolescents with chronic medical conditions treated in a German pediatric outpatient clinic.
Tópico(s): COVID-19 and Mental Health
2024 - Elsevier BV | Journal of Adolescent Health
Reinhard Rachel, Andreas Engel, Wolfgang Baumeister,
Tópico(s): Enzyme Structure and Function
1983 - Oxford University Press | FEMS Microbiology Letters
Reinhard Paschke, D. Balkow, Ute Baumeister, H. Hartung, J. R. Chipperfield, Antony B. Blake, Peter G. Nelson, G. W. Gray,
Abstract Di(5-alkylsalicylidene)ethylenediaminato nickel(II) and copper(II) complexes have been synthesized and investigated. They show similar behaviour to the corresponding alkyloxy-substituted complexes—wide mesophase ranges and calamitic smectic phases. The thermal stability of the nickel(II) complexes is higher than that of the copper(II) complexes. The title compound crystallizes with one water molecule per formula unit (water—diethyl ether interface) and Z = 4 in space group P21/c. X-ray crystal ...
Tópico(s): Metal-Catalyzed Oxygenation Mechanisms
1990 - Taylor & Francis | Molecular Crystals and Liquid Crystals Incorporating Nonlinear Optics
U. Schimmelpfennig, René Zimmering, Klaus‐Dieter Schleinitz, Reinhard Stosser, Eberhard Wenschuh, U. Baumeister, H. Hartung,
Abstract Organonickel(II)‐Komplexe des Typs [NiX{C 6 H 3 (CH 2 NR 1 R 2 ) 2 ‐2,6}] (X = Halogen, OH 2 + /CF 3 SO 3 − ; R 1 R 2 Et 1 ; R 1 R 2 iPr 2 ; R 1 = Me, R 2 = Cy 3 ; (NR 1 R 2 ) = piperidino 4 ; (NR 1 R 2 ) = pyrrolidino 5 ) werden beschrieben. Die Charakterisierung erfolgte mit Hilfe der 1 H und 13 C‐NMR‐ sowie der UV/Vis‐Spektroskopie. Von 1 a (X = Br) wurde die Kristallstruktur bestimmt. Dieser Komplex kristallisiert orthorhombisch in der Raumgruppe Pbca mit a = 1 335,8(2) pm, b = 1 903,3(3) pm, c = 1 365,4(3) pm und Z = 8, er besitzt ...
Tópico(s): Metal complexes synthesis and properties
1993 - Wiley | Zeitschrift für anorganische und allgemeine Chemie
Tim U. H. Baumeister, Nico Ueberschaar, Wolfgang Schmidt‐Heck, Jan Mohr, Michael Deicke, Thomas Wichard, Reinhard Guthke, Georg Pohnert,
Tópico(s): Mass Spectrometry Techniques and Applications
2018 - Springer Science+Business Media | Metabolomics
Christina Reinauer, Anna Lena Platzbecker, Rabea Viermann, Matthias Domhardt, Harald Baumeister, Katharina Foertsch, Hannah Linderskamp, Lisa Krassuski, Doris Staab, Kirsten Minden, Reinhold Kilian, Reinhard W. Holl, Petra Warschburger, Thomas Meißner,
Despite the high prevalence of anxiety and depression in youths with chronic medical conditions (CMCs), physicians encounter substantial barriers in motivating these patients to access mental health care services.To determine the efficacy of motivational interviewing (MI) training for pediatricians in increasing youths' use of mental health care.The COACH-MI (Chronic Conditions in Adolescents: Implementation and Evaluation of Patient-Centered Collaborative Healthcare-Motivational Interviewing) study ...
Tópico(s): Child and Adolescent Psychosocial and Emotional Development
2021 - American Medical Association | JAMA Network Open
J. S. Müller, Sofia Petrova, Reinhard Kiefer, Rolf Stucka, Christoph König, Sarah Baumeister, Angela Huebner, Hanns Lochmüller, Angela Abicht,
Congenital myasthenic syndromes (CMS) with deficiency of endplate acetylcholinesterase (AChE) are caused by mutations in the synapse specific collagenic tail subunit gene (COLQ) of AChE. We identified a novel missense mutation (T441A) homozygously in three CMS patients from two unrelated German families. The mutation is located in the C-terminal region of the ColQ protein, which initiates assembly of the triple helix, and is essential for insertion of the tail subunit into the basal lamina. Density ...
Tópico(s): Cancer Treatment and Pharmacology
2004 - Thieme Medical Publishers (Germany) | Neuropediatrics
Rudo Grimm, Hapreet Singh, Reinhard Rachel, Dieter Typke, Wolfram Zillig, Wolfgang Baumeister,
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a resolution of 20–40 nm from tilt series comprising 50–140 images. The dose was kept within tolerable limits. A resolution of 20 nm allowed visualization of the individual stalks of the S-layer of Pyrobaculum aerophilum cells, which had undergone partial ...
Tópico(s): Electron and X-Ray Spectroscopy Techniques
1998 - Elsevier BV | Biophysical Journal
Reinhard Rachel, U. Jakubowski, Wolfgang Baumeister,
SUMMARY A rapid cooling/cryotransfer system was designed to achieve a high reproducibility in vitrifying thin water films containing biological specimens. In order to improve the contrast these unstained specimens were deliberately freeze‐dried in situ in the electron microscope. The preservation of the structure obtained by freeze‐drying at 180 K and subsequent microscopy at 90 K is very encouraging as has been shown with two types of test specimen. Tubular photosynthetic membranes were used to ...
Tópico(s): Digital Holography and Microscopy
1986 - Wiley | Journal of Microscopy
W. Baumeister, Reinhard Rachel, R. Guckenberger, R. Hegerl,
Introduction Correlation averaging (CAV) is meanwhile an established technique in image processing of two-dimensional crystals /1,2/. The basic idea is to detect the real positions of unit cells in a crystalline array by means of correlation functions and to average them by real space superposition of the aligned motifs. The signal-to-noise ratio improves in proportion to the number of motifs included in the average. Unlike filtering in the Fourier domain, CAV corrects for lateral displacements of ...
Tópico(s): Advanced X-ray Imaging Techniques
1986 - Cambridge University Press | Proceedings annual meeting Electron Microscopy Society of America
Christiane Baumeister, Reinhard Ellwanger, Lutz Kilian,
It is commonly believed that the response of the price of corn ethanol (and hence of the price of corn) to shifts in biofuel policies operates in part through market expectations and shifts in storage demand, yet to date it has proved difficult to measure these expectations and to empirically evaluate this view. We utilize a recently proposed methodology to estimate the market’s expectations of the prices of ethanol, unfinished motor gasoline and crude oil at horizons from three months to one year. ...
Tópico(s): Global Energy and Sustainability Research
2016 - RELX Group (Netherlands) | SSRN Electronic Journal