Simona Baldassa, Alessandra Maria Calogero, Graziano Colombo, Renata Zippel, Nerina Gnesutta,
Abstract The serine/threonine kinase PAK4 is a Rho GTPases effector protein implicated in many critical biological processes, including regulation of cell morphology and motility, embryonic development, cell survival, response to infection, and oncogenic transformation. Consistently with its pro‐oncogenic features, PAK4 was found to be overexpressed in many cancer cell lines and tissues, and to be necessary to promote activation of survival pathways. PAK4, like other Paks, is now considered a promising ...
Tópico(s): Cardiomyopathy and Myosin Studies
2010 - Wiley | Journal of Cellular Physiology
Produção Nacional
Revisado por pares
Carlos A. Soriano de Souza, Ana Paula Vieira Colombo, Renata Souto, Carina Maciel Silva‐Boghossian, José Mauro Granjeiro, Gutemberg Gomes Alves, Alexandre Malta Rossi, Maria Helena M. Rocha‐Leão,
The kinetic of chlorhexidine digluconate (CHXDG) uptake from aqueous solution by hydroxyapatite (HA) was investigated by ultraviolet (UV) analysis performed in HA powder (UV-solid) after the CHX adsorption. Adsorption isotherm of chlorhexidine (CHX) uptake was modeled by a combination of Languimir and Langmuir–Freundlich mechanisms. Strong molecule–molecule interactions and positive cooperativity predominated in the surface when CHX concentration was above 8.6 μgCHX/mgHA. UV-solid spectra (shape, intensity ...
Tópico(s): Bone Tissue Engineering Materials
2011 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Produção Nacional
Revisado por pares
Ana Paula Vieira Colombo, Renata Souto, Carina Maciel Silva‐Boghossian, Rachel Miranda, Talita Gomes Baêta Lourenço,
The oral microbiome plays a crucial role in the establishment and maintenance of oral health. Major disturbances in the interactions between this microbiome, the microenvironment, and the host may lead to the development of oral diseases. A large proportion of infectious diseases that affect humans are caused by organisms living in biofilms. This is also the case for oral infections such as dental caries, periodontal diseases, and endodontic and fungal infections. In this review, new concepts regarding ...
Tópico(s): HIV/AIDS oral health manifestations
2014 - Springer Science+Business Media | Current Oral Health Reports
Produção Nacional
Revisado por pares
Sérgio Ruffo Roberto, Wellington Fernando Silva Borges, Ronan Carlos Colombo, Renata Koyama, Ibrar Hussain, Reginaldo Teodoro de Souza,
The objective of this study was to evaluate techniques, such as flower-cluster and berry-cluster thinning at different times, to prevent bunch compactness of 'BRS Vitoria', a new black seedless grape. The experiment was conducted during two consecutive seasons (2013 and 2014) in a commercial vineyard located in Marialva, PR, Brazil. The grapevines were trained in an overhead trellis system, spaced at 2.5 × 5.0 m. The experimental design used was randomized blocks with four replications and six treatments: ...
Tópico(s): Postharvest Quality and Shelf Life Management
2015 - Elsevier BV | Scientia Horticulturae
Chiara Paiardi, Fiorella Belotti, Sonia Colombo, Renata Tisi, Enzo Martegani,
The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor for Ras proteins whose catalytic domain is highly homologous to Ras-guanine nucleotide exchange factors from higher eukaryotes. In this study, glucose-induced Ras activation and cAMP response were investigated in mutants lacking the N-terminal domain of Cdc25 or where the entire CDC25 coding sequence was substituted by an expression cassette for a mammalian guanine nucleotide exchange factor catalytic domain. Our ...
Tópico(s): Enzyme Structure and Function
2007 - Oxford University Press | FEMS Yeast Research
Produção Nacional
Revisado por pares
Ana Paula Vieira Colombo, Renata Souto, Lélia Lima Araújo, Laís Christina Pontes Espíndola, Fátima Aparecida Rocha Resende Hartenbach, Clarissa Bichara Magalhães, Gabrielle da Silva Oliveira Alves, Talita Gomes Baêta Lourenço, Carina Maciel Silva‐Boghossian,
The dysbiotic biofilm of periodontitis may function as a reservoir for opportunistic human pathogens of clinical relevance. This study explored the virulence and antimicrobial susceptibility of staphylococci isolated from the subgingival biofilm of individuals with different periodontal conditions. Subgingival biofilm was obtained from 142 individuals with periodontal health, 101 with gingivitis and 302 with periodontitis, and cultivated on selective media. Isolated strains were identified by mass ...
Tópico(s): Streptococcal Infections and Treatments
2023 - Nature Portfolio | Scientific Reports
Valerio Leoni, G Puccetti, Renata Colombo, A.M. D'Ovidio,
The application of Tenax to the extraction of organic micro-pollutants such as pesticides and polychlorobiphenyls from waters has been studied, and it has been observed that also in the presence of other pollutants (oil, surface-active substances, etc.) the results obtained with an absorption column of Tenax-Celite are equivalent to those obtained with the continuous liquid-liquid extraction technique. For natural waters that contain solids in suspension that adsorb pesticides, it may be necessary ...
Tópico(s): Water Quality Monitoring Technologies
1976 - Elsevier BV | Journal of Chromatography A
D. Cova, Renata Colombo, G. Cellini,
A rapid and sensitive gas chromatographic method for the determination of the beta-adrenergic agent isoxsuprine in human plasma has been developed. The procedure involves the extraction of the drug with ether and an internal standard (propranolol) from plasma at alkaline pH, solvent evaporation, and the formation of a tri-trifluoroacetyl derivative by reaction with trifluoroacetic anhydride in ethyl acetate. Analyses were carried out by gas-liquid chromatography on a 3% OV-17 column using an electron ...
Tópico(s): Analytical Methods in Pharmaceuticals
1983 - Karger Publishers | Pharmacology
Produção Nacional
Revisado por pares
Renata Colombo, Janete H. Yariwake, Emerson Ferreira Queiroz, Karine Ndjoko, Kurt Hostettmann,
This work describes the on-line characterization of minor flavones from sugarcane (Saccharum officinarum) juice by high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry (LC/UV/MS) using atmospheric pressure chemical ionization-collision-induced dissociation (APCI-CID-MS/MS) and post-column derivatization using UV shift reagents. HPLC-UV analysis with shift reagents provided information about the substitution pattern in the flavonoid skeleton and, combined ...
Tópico(s): Chromatography in Natural Products
2009 - Brazilian Chemical Society | Journal of the Brazilian Chemical Society
Produção Nacional
Revisado por pares
Renata Colombo, Andrea N. L. Batista, Helder Lopes Teles, Geraldo Humberto Silva, Giovani C. C. Bomfim, Rosilene Cristina Rossetto Burgos, Alberto José Cavalheiro, Vanderlan da Silva Bolzani, Dulce Helena Siqueira Silva, Clícia R. Pelícia, Fabiana M. Guimarães, Maria Célia H. Heimberg,
Abstract Phyllanthus niruri L., commonly known in Brazil as ‘quebra‐pedra’, has long been used in the treatment of diverse diseases and especially urolithiasis. The therapeutic effects of P. niruri are attributed to various compounds present in the plant, including the hydrolysable tannin corilagin. In the present study, high‐performance liquid chromatography (HPLC‐/PAD) profiles of leaves and commercial extracts of P. niruri were examined and three compounds, found to be present in all of the samples ...
Tópico(s): Ethnobotanical and Medicinal Plants Studies
2009 - Wiley | Biomedical Chromatography
Produção Nacional
Revisado por pares
Renata Colombo, Janete H. Yariwake, Michael McCullagh,
The flavonoids present in sugarcane (Saccharum officinarum) extracts were analyzed by liquid chromatography - mass spectrometry (LC-MS), and a study of the fragmentation patterns of selected flavonoids was conducted using orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS). Seven C- and O-glycosylflavones were identified in the extracts, namely, schaftoside, isoschaftoside, luteolin-8-C-(rhamnosylglucoside), vitexin, orientin, tricin-7-O-neohesperidoside and ...
Tópico(s): Phytochemical Studies and Bioactivities
2008 - Brazilian Chemical Society | Journal of the Brazilian Chemical Society
Produção Nacional
Revisado por pares
Fabiana C. Vila, Renata Colombo, Tatiana Onofre de Lira, Janete H. Yariwake,
The antioxidant activity of sugarcane (Saccharum officinarum L.) juice towards DPPH reagent was determined (EC50) and the main compounds with radical scavenging activity in juice and leaves extracts were identified by HPLC-UV/PAD analysis combined with HPLC microfractionation monitored by TLC using β-carotene and DPPH as the detection reagents. In sugarcane leaves, luteolin-8-C-(rhamnosylglucoside) (1) was the most important compound with radical scavenging activity; in sugarcane juice, the flavones ...
Tópico(s): Natural Products and Biological Research
2008 - Brazilian Chemical Society | Journal of the Brazilian Chemical Society
Produção Nacional
Revisado por pares
Renata Colombo, Tanare C.R. Ferreira, Suellen Aparecida Alves, Marcos R.V. Lanza,
The aim of the study was to evaluate the efficiencies of photo-Fenton (Fe2+) and (Fe3+) processes in the degradation of high-concentrations of esfenvalerate (in the form of aqueous emulsion of a commercial formulation) using a recirculation flow-through photoreactor irradiated with UV light from a 15 W lamp (254 nm emission peak). The results obtained using a basic photo-Fenton (Fe2+) reaction (esfenvalerate 17 mg L−1; ferrous sulphate 1 mM; hydrogen peroxide 25 mM; pH 2.5) were compared with those acquired ...
Tópico(s): Insect and Pesticide Research
2011 - Elsevier BV | Journal of Hazardous Materials
Produção Nacional
Revisado por pares
Renata Colombo, Janete H. Yariwake, Emerson Ferreira Queiroz, Karine Ndjoko, Kurt Hostettmann,
Abstract HPLC‐UV‐MS (APCI‐MS/MS and CID/MS) was utilised for the identification of eight additional flavone glycosides from sugarcane ( Saccharum officinarum L., Gramineae) extracts of bagasse, leaves and juice (‘garapa’). Relevant information about substitution patterns was obtained through UV detection using post‐column addition of shift reagents, while tandem MS provided structural information confirming the proposed structures of the C ‐glycosides vitexin, orientin, luteolin‐8‐ C ‐(rhamnosylglucoside), ...
Tópico(s): Chromatography in Natural Products
2006 - Wiley | Phytochemical Analysis
Produção Nacional
Revisado por pares
Renata Colombo, Fernando Mauro Lanças, Janete H. Yariwake,
A high-performance liquid chromatography (HPLC) method with photo-diode array (DAD) detection was developed to separate and quantify flavonoids in sugarcane leaves and bagasse (= the crushed sugarcane refuse from juice extraction), and in sugarcane juice. Sugarcane flavonoids consist of a complex mixture of aglycones and glycosides (including flavonolignan glycosides), and the HPLC-UV method herein proposed is suitable for their quantification as total flavonoids. This method was applied to analyze samples ...
Tópico(s): Biofuel production and bioconversion
2005 - Elsevier BV | Journal of Chromatography A
Produção Nacional
Revisado por pares
Renata Colombo, Janete H. Yariwake, Emerson Ferreira Queiroz, Karine Ndjoko, Kurt Hostettmann,
Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization–MS/MS and collision-induced dissociation–MS). On-line UV and MS data demonstrated ...
Tópico(s): Phytochemical Studies and Bioactivities
2005 - Elsevier BV | Journal of Chromatography A
Produção Nacional
Revisado por pares
Renata Colombo, Tanare C.R. Ferreira, Suellen Aparecida Alves, Renato Lajarim Carneiro, Marcos R.V. Lanza,
Lambda-cyhalothrin is a potent pyrethroid insecticide used widely in pest management. Detectable levels of the pyrethroid in agricultural watersheds are potentially toxic to aquatic organisms. There is little information in the scientific literature about degradation in aqueous media of the Lambda-cyhalothrin by Advanced Oxidative Process. A mathematical approach for the degradation of this compound has not yet been fully explored… The Central composite design (CCD) and response surface method (RSM) ...
Tópico(s): Pesticide and Herbicide Environmental Studies
2013 - Elsevier BV | Journal of Environmental Management
Simona Rossi, A.P Dalpero, Severino Ghini, Renata Colombo, A. G. Sabatini, Stefano Girotti,
The analysis of several organophosphorus and carbamate pesticide residues in the bodies of honeybees using gas chromatography (GC) and gel permeation chromatography (GPC) clean-up is described. Freeze-dried or lyophilized insect samples were blended with diatomaceous earth (Extrelut) then underwent elution with methylene chloride. This extraction method has shown good recovery on various spike standard levels. Samples are cleaned up by GPC with a Bio Beads SX 3 column and a cyclohexane–ethylacetate ( ...
Tópico(s): Plant and animal studies
2001 - Elsevier BV | Journal of Chromatography A
Mónica Fernández-Franzón, Carolina Padrón-Sanz, L Marconi, Severino Ghini, Renata Colombo, A. G. Sabatini, Stefano Girotti,
A method based on solid-phase microextraction (SPME) followed by gas chromatography with nitrogen-phosphorus detection was developed for the purpose of determining 18 organophosphorus pesticide residues in honeybee samples (Apis mellifera). The extraction capacities of polyacrylate and poly(dimethylsiloxane) fibers were compared. The main factors affecting the SPME process, such as the absorption time profile, salt, and temperature, were optimized. The method involved honeybee sample homogenization, ...
Tópico(s): Plant and animal studies
2001 - Elsevier BV | Journal of Chromatography A
Produção Nacional
Revisado por pares
Renata Colombo, Tanare C.R. Ferreira, Renato A. Ferreira, Marcos R.V. Lanza,
Mefenamic acid (MEF) is a non-steroidal anti-inflammatory drug indicated for relief of mild to moderate pain, and for the treatment of primary dysmenorrhea. The presence of MEF in raw and sewage waters has been detected worldwide at concentrations exceeding the predicted no-effect concentration. In this study, using experimental designs, different oxidative processes (H2O2, H2O2/UV, fenton and Photo-fenton) were simultaneously evaluated for MEF degradation efficiency. The influence and interaction ...
Tópico(s): Advanced oxidation water treatment
2016 - Elsevier BV | Journal of Environmental Management
Elena Grego, Patrizia Robino, Clara Tramuta, Gessica Giusto, Michela Boi, Renata Colombo, G. Serra, S Chiadò-Cutin, Marco Gandini, Patrizia Nebbia,
Honey as a topical treatment for infected wounds dates back to ancient times. However, few studies have been reported concerning the medical properties of Italian honey. In this study, the microbial contamination, the antimicrobial activity and the antibiotic residues of 6 different varieties of Piedmont honeys were evaluated. The antimicrobial activity of honeys was tested by agar well diffusion method and 1 honey for each variety has been selected and tested by broth micro-dilution test to determine ...
Tópico(s): Essential Oils and Antimicrobial Activity
2016 - Society of Swiss Veterinarians | Schweizer Archiv für Tierheilkunde
Claudio Poggio, Marco Colombo, Carla Renata Arciola, Tiziana Greggi, Andrea Scribante, Alberto Dagna,
The latest diffusion of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the coronavirus disease (COVID-19), has involved the whole world population. Even if huge efforts to control the pandemic have been done, the viral spread is still continuing. COVID-19 is reported as a zoonosis jumped from bats and pangolins to humans. After infection in humans, SARS-CoV-2 is found in the nasopharyngeal and salivary secretions. The virus has also been detected in the blood plasma ...
Tópico(s): Dental Anxiety and Anesthesia Techniques
2020 - Multidisciplinary Digital Publishing Institute | Materials
Produção Nacional
Revisado por pares
Alana G. Souza, Giovanni F. de Lima, Renata Colombo, Derval dos Santos Rosa,
Tópico(s): Material Properties and Processing
2020 - Springer Science+Business Media | Cellulose
Produção Nacional
Revisado por pares
Caroline Cardoso de SOUZA, Catarina Amorim Oliveira, Jéssica Fontinelly PIRES, Tatiana Colombo Pimentel, Renata S.L. Raices, Luciana C. Nogueira,
This study aimed to develop and characterize mixed beverages (suchás) formulations based on organic apple juice and cardamom tea (F1= 50:50 vv−1, F2 = 60:40 vv−1 and F3 =70:30 vv−1). The formulation (F3) presented the highest total phenolic compounds content (31.79 mg EAG g-1), antioxidant activity (50.04 µg mL-1), total soluble solids (5.1 oBrix), acidity (pH 4.17 and total acidity 0.15 g malic acid 100 mL-1) and sensory acceptance (flavor and overall impression). Furthermore, it presented important volatile ...
Tópico(s): Medicinal Plants and Neuroprotection
2020 - Sociedade Brasileira de Ciência e Tecnologia de Alimentos | Food Science and Technology
Produção Nacional
Revisado por pares
Milla Araújo de Almeida, Renata Colombo,
Tópico(s): Catalysis for Biomass Conversion
2021 - Springer Science+Business Media | BioEnergy Research
Produção Nacional
Milla Araújo de Almeida, Renata Colombo,
The present paper reports the development of nutritious substrates through the use of renewable materials via vermicomposting. Vermicomposting was carried out using stacked bin composter (14.5 cm high, 78 cm wide and 30 cm long) filled with sawdust/soil mixture, fresh wastes, Eisenia fetida and dry wastes; this led to the generation of humus with chemical composition suitable for use as nutritional component (41.9% moisture; pH 7.3; 31.2% organic matter; 17.5 and 3.2% of fulvic and humic acid, respectively; ...
Tópico(s): Hygrothermal properties of building materials
2021 - Elsevier BV | Sustainable Chemistry and Pharmacy
Produção Nacional
Revisado por pares
Renata Colombo Bonadio, Ana Paula Messias, Otavio Augusto Moreira, Letícia Vecchi Leis, Bruna Orsi, Laura Testa, Maria Del Pilar Estevez Diz,
The COVID-19 pandemic has led to the need for health services adjustments, which may have compromised management of other diseases. For cancer patients, delays may significantly impair outcomes in some situations. We aimed to assess the impact of the COVID-19 pandemic in breast and cervical cancer diagnosis and treatment compared to the same period prior to the pandemic.Data were collected from patients attending their first visit to a Brazilian cancer centre from 1 September 2020 to 31 January ...
Tópico(s): Economic and Financial Impacts of Cancer
2021 - Cancer Intelligence | ecancermedicalscience
Produção Nacional
Revisado por pares
Guilherme Nader Marta, Renata Colombo Bonadio, Odeli Nicole Encinas Sejas, Gabriel Watarai, Maria Cecília Mathias-Machado, Lorena Teixeira Frasson, Camila Motta Venchiarutti Moniz, Raquel Keiko de Luca Ito, Driele Peixoto, Camilla O. Hoff, Veruska Menegatti Anastacio, Ulysses Ribeiro, Juliana Pereira, Vanderson Rocha, Edson Abdala, Maria Del Pilar Estevez Diz, Paulo M. Hoff,
Patients with cancer are at increased risk for unfavorable outcomes from COVID-19. Knowledge about the outcome determinants of severe acute respiratory syndrome coronavirus 2 infection in this population is essential for risk stratification and definition of appropriate management. Our objective was to evaluate prognostic factors for all-cause mortality in patients diagnosed with both cancer and COVID-19.
Tópico(s): Long-Term Effects of COVID-19
2021 - Lippincott Williams & Wilkins | JCO Global Oncology
Produção Nacional
Revisado por pares
Renata Colombo Bonadio, Paolo Tarantino, Laura Testa, Kevin Punie, Sònia Pernas, Carlos H. Barrios, Giuseppe Curigliano, Sara M. Tolaney, Romualdo Barroso‐Sousa,
New therapy options have changed the treatment landscape of early-stage triple-negative breast cancer (TNBC) in recent years. Most patients are candidates for neoadjuvant chemotherapy, which helps to downstage the tumor and tailor adjuvant systemic therapy based on pathologic response. Capecitabine, pembrolizumab, and olaparib have been incorporated into the armamentarium of adjuvant treatment for selected patients. The KEYNOTE-522 trial, that demonstrated the benefit of pembrolizumab, given in addition ...
Tópico(s): Advanced Breast Cancer Therapies
2022 - Elsevier BV | Cancer Treatment Reviews
Produção Nacional
Revisado por pares
Taynara Oliveira Silva, Lorena Athie Goulart, Isaac Sánchez-Montes, Géssica de Oliveira Santiago Santos, Renato Batista dos Santos, Renata Colombo, Marcos R.V. Lanza,
The present study reports the development and application of a gas diffusion electrode (GDE) based on Printex L6 carbon modified with 2.0% benzophenone-3,3′,4,4′-tetracarboxylic dianhydride (PL6C/2.0%BTDA) for the efficient electrogeneration of hydrogen peroxide (H2O2) and degradation of ciprofloxacin (CIP) using different advanced oxidation processes (AOPs). The modified GDE was studied under different current densities and 25 mA cm−2 was found to be the most efficient current density, since the application ...
Tópico(s): Advanced Photocatalysis Techniques
2022 - Elsevier BV | Chemical Engineering Journal