Produção Nacional
Revisado por pares
Hercílio Martelli Júnior, Renato Assis Machado, Paulo Rogério Ferreti Bonan, Danyel Elias da Cruz Pérez, Fábio Ramôa Pires, Fábio Abreu Alves,
... papers by this authorRenato Assis Machado, Corresponding Author Renato Assis Machado [email protected] orcid.org/0000-0002-1697-3662 ... University of Campinas (FOP/UNICAMP), Piracicaba, Brazil Correspondence Renato Assis Machado, University of Campinas (FOP/UNICAMP), 901 Limeira Avenue, ... papers by this authorRenato Assis Machado, Corresponding Author Renato Assis Machado [email protected] orcid.org/0000-0002-1697-3662 ... University of Campinas (FOP/UNICAMP), Piracicaba, Brazil Correspondence Renato Assis Machado, University of Campinas (FOP/UNICAMP), 901 Limeira Avenue, ...
Tópico(s): Oral health in cancer treatment
2020 - Wiley | Oral Diseases
Produção Nacional
Revisado por pares
Bruna G. Palm, Dimas I. Alves, Mats I. Pettersson, Viet T. Vu, Renato Machado, Renato J. Cintra, Fábio M. Bayer, Patrik Dammert, Hans Hellsten,
This paper presents five different statistical methods for ground scene prediction (GSP) in wavelength-resolution synthetic aperture radar (SAR) images. The GSP image can be used as a reference image in a change detection algorithm yielding a high probability of detection and low false alarm rate. The predictions are based on image stacks, which are composed of images from the same scene acquired at different instants with the same flight geometry. The considered methods for obtaining the ground scene ...
Tópico(s): Synthetic Aperture Radar (SAR) Applications and Techniques
2020 - Multidisciplinary Digital Publishing Institute | Sensors
Produção Nacional
Revisado por pares
S.F. Pires, Wanderson D. DaRocha, Jorge Marcelo de Freitas, Laser Antônio Machado Oliveira, Gregory T. Kitten, Carlos Renato Machado, Sérgio D.J. Pena, Egler Chiari, Andréa Mara Macedo, Santuza Maria Ribeiro Teixeira,
Different strains of Trypanosoma cruzi were transfected with an expression vector that allows the integration of green fluorescent protein (GFP) and red fluorescent protein (RFP) genes into the beta-tubulin locus by homologous recombination. The sites of integration of the GFP and RFP markers were determined by pulse-field gel electrophoresis and Southern blot analyses. Cloned cell lines selected from transfected epimastigote populations maintained high levels of fluorescent protein expression even ...
Tópico(s): Biochemical and Molecular Research
2007 - Elsevier BV | International Journal for Parasitology
Produção Nacional
Revisado por pares
Aline Araújo Zuma, Danielle Pereira Cavalcanti, Marcelo Zogovich, Ana Carolina L. Machado, I.C. Mendes, Marc Thiry, Antônio Galina, Wanderley de Souza, Carlos Renato Machado, María Cristina M. Motta,
Trypanosoma cruzi, the etiological agent of Chagas disease, exhibits a single mitochondrion with an enlarged portion termed kinetoplast. This unique structure harbors the mitochondrial DNA (kDNA), composed of interlocked molecules: minicircles and maxicircles. kDNA is a hallmark of kinetoplastids and for this reason constitutes a valuable target in chemotherapeutic and cell biology studies. In the present work, we analyzed the effects of berenil, a minor-groove-binding agent that acts preferentially ...
Tópico(s): Biochemical and Molecular Research
2014 - Springer Science+Business Media | Parasitology Research
Produção Nacional
Revisado por pares
Liane Unchalo Machado, Humberto Holmer Fiori, Matteo Baldisserotto, Pedro Celiny Ramos Garcia, Ana Cláudia Vieira, Renato Machado Fiori,
To evaluate surfactant production and function in term neonates with transient tachypnea of the newborn (TTN).Samples of gastric aspirates collected within 30 minutes of birth from 42 term newborns with gestational age ≥ 37 weeks (21 patients with TTN and 21 control subjects), delivered via elective cesarean delivery, were analyzed with lamellar body count and stable microbubble test.Results of lamellar body counts and stable microbubble tests were significantly lower in the TTN group than in control ...
Tópico(s): Congenital Diaphragmatic Hernia Studies
2011 - Elsevier BV | The Journal of Pediatrics
Produção Nacional
Revisado por pares
Alice Machado-Silva, Santuza Maria Ribeiro Teixeira, Glória Regina Franco, Andréa Mara Macedo, Sérgio D.J. Pena, Richard McCulloch, Carlos Renato Machado,
Trypanosomes are unicellular eukaryotes that cause disease in humans and other mammals. Trypanosoma cruzi and Trypanosoma brucei are the causative agents, respectively, of Chagas disease in the Americas and sleeping sickness in sub-Saharan Africa. To better comprehend the interaction of these parasites with their hosts, understanding the mechanisms involved in the generation of genetic variability is critical. One such mechanism is mismatch repair (MMR), which has a crucial, evolutionarily conserved ...
Tópico(s): Helminth infection and control
2008 - Elsevier BV | Gene
Produção Nacional
Revisado por pares
Alice Machado-Silva, Paula Gonçalves Cerqueira, Viviane Grazielle-Silva, Fernanda Ramos Gadelha, Eduardo de Figueiredo Peloso, Santuza Maria Ribeiro Teixeira, Carlos Renato Machado,
Trypanosoma cruzi, the causative agent of Chagas disease, is an obligatory intracellular parasite with a digenetic life cycle. Due to the variety of host environments, it faces several sources of oxidative stress. In addition to reactive oxygen species (ROS) produced by its own metabolism, T. cruzi must deal with high ROS levels generated as part of the host's immune responses. Hence, the conclusion that T. cruzi has limited ability to deal with ROS (based on the lack of a few enzymes involved with ...
Tópico(s): Parasites and Host Interactions
2015 - Elsevier BV | Mutation Research/Reviews in Mutation Research
Produção Nacional
Revisado por pares
Clarisse Bavoux, Andréia Machado Leopoldino, Valérie Bergoglio, Jiyang O‐Wang, Tomoo Ogi, Anne Bieth, Jean‐Gabriel Judde, Sérgio Danilo Junho Pena, Marie‐France Poupon, Thomas Helleday, Masatoshi Tagawa, Carlos Renato Machado, Jean‐Sébastien Hoffmann, Christophe Cazaux,
Abstract It is currently widely accepted that genetic instability is key to cancer development. Many types of cancers arise as a consequence of a gradual accumulation of nucleotide aberrations, each mutation conferring growth and/or survival advantage. Genetic instability could also proceed in sudden bursts leading to a more drastic upheaval of structure and organization of the genome. Genetic instability, as an operative force, will produce genetic variants and the greater the instability, the ...
Tópico(s): Cancer Genomics and Diagnostics
2005 - American Association for Cancer Research | Cancer Research
Produção Nacional
Revisado por pares
Priscila C. Campos, Viviane G. Silva, Carolina Furtado, Alice Machado-Silva, Wanderson D. DaRocha, Eduardo de Figueiredo Peloso, Fernanda Ramos Gadelha, Marisa Helena Gennari de Medeiros, Gustavo de Carvalho Lana, Ying Chen, Rebecca L. Barnes, Danielle G. Passos-Silva, Richard McCulloch, Carlos Renato Machado, Santuza Maria Ribeiro Teixeira,
Components of the DNA mismatch repair (MMR) pathway are major players in processes known to generate genetic diversity, such as mutagenesis and DNA recombination. Trypanosoma cruzi, the protozoan parasite that causes Chagas disease has a highly heterogeneous population, composed of a pool of strains with distinct characteristics. Studies with a number of molecular markers identified up to six groups in the T. cruzi population, which showed distinct levels of genetic variability. To investigate the ...
Tópico(s): Helminth infection and control
2010 - Elsevier BV | Molecular and Biochemical Parasitology
Marcos Szeinfeld, I. H. Ihmedan, Marvin Moser, Renato Machado, K. Klose, A. N. Serafini,
Szeinfeld, M.; Ihmedan, I. H.; Moser, M. M.; Machado, R.; Klose, K. H.; Serafini, A. N. Author Information
Tópico(s): Spinal Hematomas and Complications
1986 - Lippincott Williams & Wilkins | Obstetric Anesthesia Digest
Produção Nacional
Revisado por pares
Jorge Marcelo de Freitas, Luciana de Oliveira Andrade, Simone F. Pires, Ricardo Santana de Lima, Egler Chiari, Ricardo Ribeiro dos Santos, Milena Botelho Pereira Soares, Carlos Renato Machado, Glória Regina Franco, Sérgio D.J. Pena, Andréa Mara Macedo,
We have previously demonstrated that both parasite genetic variability and host genetic background were important in determining the differential tissue distribution of the Col1.7G2 and JG T. cruzi monoclonal strains after artificial infections in mice. We observed that the JG strain was most prevalent in hearts of mouse lineages with the MHC haplotype H-2d (BALB/c and DBA2), while Col1.7G2 was predominant in hearts from C57BL/6 mice, which have the H-2b haplotype. To assess whether the MHC gene ...
Tópico(s): Toxoplasma gondii Research Studies
2009 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Michelle Barbi de Moura, Bruno Luiz Fonseca Schamber‐Reis, Danielle Gomes Passos Silva, Matheus Andrade Rajão, Andréa Mara Macedo, Glória Regina Franco, Sérgio Danilo Junho Pena, Santuza Maria Ribeiro Teixeira, Carlos Renato Machado,
Abstract We report the cloning and characterization of the DNA polymerase η gene from Trypanosoma cruzi ( TcPolη ), the causative agent of Chagas disease. This protein, which can bypass cyclobutane pyrimidine dimers, contains motifs that are conserved between Y family polymerases. In vitro assays showed that the recombinant protein is capable of synthesizing DNA in undamaged primer‐templates. Intriguingly, T. cruzi overexpressing TcPolη does not increase its resistance to UV‐light (with or without caffeine) ...
Tópico(s): Biochemical and Molecular Research
2009 - Wiley | Environmental and Molecular Mutagenesis
Produção Nacional
Revisado por pares
Daniella Alchaar D’Ávila, Andréa Mara Macedo, Helder Magno Silva Valadares, Eliane Dias Gontijo, Ana Maria de Castro, Carlos Renato Machado, Egler Chiari, Lúcia Maria da Cunha Galvão,
ABSTRACT Our research aimed to characterize the genetic profiles of 102 Trypanosoma cruzi isolates recently obtained from 44 chronic chagasic patients from different regions of the states of Minas Gerais and Goiás in Brazil. At least two isolates were obtained from each patient at different times in order to study the parasite population dynamics during disease progression in the chronic phase. The isolates were characterized molecularly by genotyping the 3′ region of the 24Sα rRNA, the mitochondrial ...
Tópico(s): Insect symbiosis and bacterial influences
2009 - American Society for Microbiology | Journal of Clinical Microbiology
Produção Nacional
Revisado por pares
Sérgio D.J. Pena, Carlos Renato Machado, Andréa Mara Macedo,
Although the genome of Trypanosoma cruzi has been completely sequenced, little is known about its population structure and evolution. Since 1999, two major evolutionary lineages presenting distinct epidemiological characteristics have been recognised: T. cruzi I and T. cruzi II. We describe new and important aspects of the population structure of the parasite, and unequivocally characterise a third ancestral lineage that we propose to name T. cruzi III. Through a careful analysis of haplotypes (blocks ...
Tópico(s): Helminth infection and control
2009 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Daniel Barbosa, Renato Machado Monaro, Denis V. Coury, Mário Oleskovicz,
This research presents a method for frequency estimation in power systems using an adaptive filter based on the Least Mean Square Algorithm (LMS). In order to analyze a power system, three-phase voltages were converted into a complex signal applying the αβ-transform and the results were used in an adaptive filtering algorithm. Although the use of the complex LMS algorithm is described in the literature, this paper deals with some practical aspects of the algorithm implementation. In order to reduce ...
Tópico(s): Power Line Communications and Noise
2009 - Elsevier BV | International Journal of Electrical Power & Energy Systems
Produção Nacional
Revisado por pares
Bianca Zingales, Andrade Sg, Marcelo R. S. Briones, D.M. Campbell, Egler Chiari, O. Fernandes, Felipe Guhl, E. Lages‐Silva, A. A. P. Macedo, Carlos Renato Machado, MA Miles, A J Romanha, NR Sturm, Michel Tibayrenc, A.G. Schijman,
In an effort to unify the nomenclature of Trypanosoma cruzi, the causative agent of Chagas disease, an updated system was agreed upon at the Second Satellite Meeting.A consensus was reached that T. cruzi strains should be referred to by six discrete typing units (T.cruzi I-VI).The goal of a unified nomenclature is to improve communication within the scientific community involved in T. cruzi research.The justification and implications will be presented in a subsequent detailed report.
Tópico(s): Helminth infection and control
2009 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Francisco Pereira Lobo, Bruno Eduardo Fernandes Mota, Sérgio D.J. Pena, Vasco Azevedo, Andréa Mara Macedo, Andreas Tauch, Carlos Renato Machado, Glória Regina Franco,
Virus-host biological interaction is a continuous coevolutionary process involving both host immune system and viral escape mechanisms. Flaviviridae family is composed of fast evolving RNA viruses that infects vertebrate (mammals and birds) and/or invertebrate (ticks and mosquitoes) organisms. These host groups are very distinct life forms separated by a long evolutionary time, so lineage-specific anti-viral mechanisms are likely to have evolved. Flaviviridae viruses which infect a single host lineage ...
Tópico(s): Viral Infections and Vectors
2009 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Magda Lahorgue Nunes, Fabiana Eloisa Mugnol, Igor Bica, Renato Machado Fiori,
Pyridoxine dependency and congenital hypophosphatasia are unusual metabolic disorders. We report a female infant born from healthy consanguineous parents with shortening of limbs, detected during pregnancy by ultrasonography. Immediately after delivery, the baby was admitted to the neonatal intensive care unit because of respiratory distress. A bone radiograph showed hypomineralization of all bones, and serum alkaline phosphatase was very low (10 U/L). Within the first day of life, seizures (focal ...
Tópico(s): Bone health and treatments
2002 - SAGE Publishing | Journal of Child Neurology
Produção Nacional
Revisado por pares
P.R. Einloft, Pedro Celiny Ramos Garcia, Jefferson Pedro Piva, Francisco Bruno, Délio José Kipper, Renato Machado Fiori,
OBJETIVO: Revisar e descrever os dados epidemiológicos dos pacientes admitidos em uma unidade de terapia pediátrica brasileira (UTIP) e compará-los aos aspectos clínicos associados aos índices de gravidade e mortalidade. Descrever as características desses pacientes, incluindo os dados demográficos, prevalência de doenças, índices de mortalidade e fatores associados. MÉTODOS: Os dados foram coletados retrospectivamente de todos os pacientes admitidos na UTIP de um hospital universitário entre 1978 ...
Tópico(s): Neonatal Respiratory Health Research
2002 - UNIVERSIDADE DE SÃO PAULO | Revista de Saúde Pública
Produção Nacional
Revisado por pares
Humberto Holmer Fiori, Ioanna Varela, Adriana Bosquê Justo, Renato Machado Fiori,
Aims: To determine the usefulness of the stable microbubble test (SMT) and of the click test (CT) on gastric aspirates obtained soon after birth to predict respiratory distress syndrome (RDS) in preterm babies not requiring ventilation at birth. Patients and methods: The study was carried out with a cohort between 24 and 34 weeks of gestational age. Gastric secretions were collected before 1 hour of life and frozen for further analysis. Results: 110 neonates were studied. For a cut-off value ≤ 10 microbubbles/ ...
Tópico(s): Respiratory Support and Mechanisms
2003 - De Gruyter | Journal of Perinatal Medicine
Produção Nacional
Revisado por pares
Luiz Augusto-Pinto, Santuza Maria Ribeiro Teixeira, Sérgio D.J. Pena, Carlos Renato Machado,
Abstract We have identified single-nucleotide polymorphisms (SNPs) in the mismatch-repair gene TcMSH2 from Trypanosoma cruzi. Phylogenetic inferences based on the SNPs, confirmed by RFLP analysis of 32 strains, showed three distinct haplogroups, denominated A, B, and C. Haplogroups A and C presented strong identity with the previously described T. cruzi lineages I and II, respectively. A third haplogroup (B) was composed of strains presenting hybrid characteristics. All strains from a haplogroup encoded ...
Tópico(s): Genetic factors in colorectal cancer
2003 - Oxford University Press | Genetics
Produção Nacional
Revisado por pares
Débora de Oliveira Lopes, Bruno Luiz Fonseca Schamber‐Reis, Carlos Gustavo Regis-da-Silva, Matheus Andrade Rajão, Wanderson D. DaRocha, Andréa Mara Macedo, Glória Regina Franco, Sheila Cristina Nardelli, Sérgio Schenkman, Jean‐Sébastien Hoffmann, Christophe Cazaux, Sérgio Danilo Junho Pena, Santuza Maria Ribeiro Teixeira, Carlos Renato Machado,
Mammalian DNA polymerase β is a nuclear enzyme involved in the base excision and single-stranded DNA break repair pathways. In trypanosomatids, this protein does not have a defined cellular localization, and its function is poorly understood. We characterized two Trypanosoma cruzi proteins homologous to mammalian DNA polymeraseβ, TcPolβ and TcPolβPAK, and showed that both enzymes localize to the parasite kinetoplast. In vitro assays with purified proteins showed that they have DNA polymerization ...
Tópico(s): HIV/AIDS drug development and treatment
2008 - Elsevier BV | DNA repair
Produção Nacional
Revisado por pares
Matheus Andrade Rajão, Danielle G. Passos-Silva, Wanderson D. DaRocha, Glória Regina Franco, Andréa Mara Macedo, Sérgio D.J. Pena, Santuza Maria Ribeiro Teixeira, Carlos Renato Machado,
DNA polymerase kappa (Pol kappa) is a low-fidelity polymerase that has the ability to bypass several types of lesions. The biological role of this enzyme, a member of the DinB subfamily of Y-family DNA polymerases, has remained elusive. In this report, we studied one of the two copies of Pol kappa from the protozoan Trypanosoma cruzi (TcPol kappa). The role of this TcPol kappa copy was investigated by analysing its subcellular localization, its activities in vitro, and performing experiments with ...
Tópico(s): Insect symbiosis and bacterial influences
2008 - Wiley | Molecular Microbiology
Produção Nacional
Revisado por pares
Rogerio Tessler, Mariutska Zadinello, Humberto Holmer Fiori, Maurício Obal Colvero, Jaques Belik, Renato Machado Fiori,
Objectives: Sildenafil, a phosphodiesterase-5 inhibitor, significantly improves oxygenation when used in animal models and patients with pulmonary hypertension. Tadalafil is a new and clinically available phosphodiesterase-5 inhibitor that, aside from causing pulmonary vasodilation, has been shown to increase cardiac output in pulmonary hypertensive adults. Its hemodynamic effects on the newborn, however, have not been tested. The objective was to evaluate the effect of tadalafil on central hemodynamics ...
Tópico(s): Cardiac, Anesthesia and Surgical Outcomes
2008 - Lippincott Williams & Wilkins | Pediatric Critical Care Medicine
Produção Nacional
Revisado por pares
Antônia Claudia Jácome da Câmara, Adalberto A. Varela-Freire, Helder Magno Silva Valadares, Andréa Mara Macedo, Daniella Alchaar D’Ávila, Carlos Renato Machado, E. Lages‐Silva, Egler Chiari, Lúcia Maria da Cunha Galvão,
Trypanosoma cruzi genetic diversity was investigated in 25 isolates (vectors and humans) from the semiarid zone of the State of Rio Grande do Norte, Brazil. Molecular markers (3' region of the 24Salpha rRNA; mitochondrial cytochrome oxidase subunit 2 (COII) gene; spliced leader intergenic region (SL-IR) gene; allelic size microsatellite polymorphism) identified 56% TcIII (100% Panstrongyluslutzi; 50% Triatomabrasiliensis); 40% TcII (91.7% humans; 50% T. brasiliensis) and 4% TcI (human). Microsatellite ...
Tópico(s): Helminth infection and control
2010 - Elsevier BV | Acta Tropica
Produção Nacional
Revisado por pares
Ricardo Garcia Branco, Lisandra Dias Xavier, Pedro Celiny Ramos Garcia, Jefferson Pedro Piva, Humberto Holmer Fiori, Matteo Baldisserotto, Renato Machado Fiori, Robert C. Tasker,
To evaluate the feasibility and safe operationalization of a pediatric glycemic control protocol in the setting of a general pediatric intensive care unit in a developing country.Prospective, observational cohort study carried out over 12 months.Fourteen-bed pediatric intensive care unit in Brazil.Children requiring mechanical ventilation with at least one organ system dysfunction were included.Glucose was monitored and insulin used for persistent hyperglycemia (glucose >140 mg/dL [7.8 mmol/L] for ...
Tópico(s): Diabetes Management and Research
2010 - Lippincott Williams & Wilkins | Pediatric Critical Care Medicine
Produção Nacional
Revisado por pares
Claudiney Melquíades Rodrigues, Helder Magno Silva Valadares, Amanda Fortes Francisco, Jerusa Marilda Arantes, Camila França Campos, Andréa Teixeira−Carvalho, Olindo Assis Martins‐Filho, Márcio Sobreira Silva Araújo, Rosa Maria Esteves Arantes, Egler Chiari, Glória Regina Franco, Carlos Renato Machado, Sérgio Danilo Junho Pena, Ana Maria Caetano Faria, Andréa Mara Macedo,
A century after the discovery of Trypanosoma cruzi in a child living in Lassance, Minas Gerais, Brazil in 1909, many uncertainties remain with respect to factors determining the pathogenesis of Chagas disease (CD). Herein, we simultaneously investigate the contribution of both host and parasite factors during acute phase of infection in BALB/c mice infected with the JG and/or CL Brener T. cruzi strains. JG single infected mice presented reduced parasitemia and heart parasitism, no mortality, levels ...
Tópico(s): Parasites and Host Interactions
2010 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Danielle G. Passos-Silva, Matheus Andrade Rajão, Pedro Henrique Nascimento de Aguiar, João P. Vieira‐da‐Rocha, Carlos Renato Machado, Carolina Furtado,
A wide variety of DNA lesions arise due to environmental agents, normal cellular metabolism, or intrinsic weaknesses in the chemical bonds of DNA. Diverse cellular mechanisms have evolved to maintain genome stability, including mechanisms to repair damaged DNA, to avoid the incorporation of modified nucleotides, and to tolerate lesions (translesion synthesis). Studies of the mechanisms related to DNA metabolism in trypanosomatids have been very limited. Together with recent experimental studies, ...
Tópico(s): Biochemical and Molecular Research
2010 - Hindawi Publishing Corporation | Journal of Nucleic Acids
Produção Nacional
Revisado por pares
Jefferson Pedro Piva, Patrícia M. Lago, Jairo Othero, Pedro Celiny Ramos Garcia, Renato Machado Fiori, Humberto Holmer Fiori, Luiz Alexandre Alegretti Borges, Fernando Suparregui Dias,
Objective To evaluate the modes of death and treatment offered in the last 24 h of life to patients dying in 10 Brazilian intensive care units (ICUs) over a period of 2 years. Design and setting Cross-sectional, multicentre, retrospective study based on medical chart review. The medical records of all patients that died in seven paediatric and three adult ICUs belonging to university and tertiary hospitals over a period of 2 years were included. Deaths in the first 24 h of admission to the ICU and ...
Tópico(s): Grief, Bereavement, and Mental Health
2010 - BMJ | Journal of Medical Ethics
Produção Nacional
Revisado por pares
Inah Westphal Batista da Silva Daniel, Humberto Holmer Fiori, Jefferson Pedro Piva, Terezinha Paz Muñhoz, André Valiente Nectoux, Renato Machado Fiori,
Lamellar body count (LBC) in amniotic fluid is being used to identify infants at risk of respiratory distress syndrome (RDS) who would benefit from surfactant prophylaxis or very early therapy. The test in gastric aspirates of newborns has not been properly explored.The main objective of this research was to evaluate the performance of LBC alone or in combination with the stable microbubble test (SMT), done on gastric aspirates from preterm babies to predict RDS.A total of 34 preterm infants with RDS ...
Tópico(s): Neuroscience of respiration and sleep
2010 - Karger Publishers | Neonatology