Record Anti-Slavery Reporter p 365 1841 Dec 23. , quotation from the concerning the purchase of slaves from the King of Ashanti for ...
1841 - Gale Group | SAS Part 1
Xavier C. Ding, Helge Großhans,
... and/or targets. Alternatively, the transfected miRNA target reporters that were used in the bulk of studies ... 2007) and choice of the promoters that drive reporter gene expression (Kong et al, 2008) can affect ... proteins ALG-1/-2 and causes upregulation of reporter genes under miRNA control (Ding et al, 2005; ... confer let-7-mediated regulation on an unrelated reporter gene (Slack et al, 2000). To verify that ... UTR, we employed transgenic animals expressing a lacZ reporter gene fused to the lin-41 3′UTR ... 7-binding sites. (A) Schematic representation of the reporter strains. Black square, WT N2; Is[col-10∷ ...
Tópico(s): MicroRNA in disease regulation
2009 - Springer Nature | The EMBO Journal
Record Anti-Slavery Reporter p 401 1842 Jan 3. extract quoted from the , about the dismissal of the British consul in Tunis for ...
1842 - Gale Group | SAS Part 1
Nobuaki Eto, Makoto Miyagishi, Reiko Inagi, Toshiro Fujita, Masaomi Nangaku,
... in a hypoxic milieu, we constructed a luciferase reporter vector by incorporating the promoter region of human ... a hypoxic milieu, we constructed a second luciferase reporter vector by incorporating seven tandem repeats of HIF- ... were considered to be those which suppressed VEGF reporter activity in comparison with the negative control in ... 0.05 were considered statistically significant. A VEGF-reporter vector was obtained using a 2.7-kbp ... assay, HEK293T cells were transiently transfected with VEGF reporter vector and were subjected to a graded oxygen ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2009 - Elsevier BV | American Journal Of Pathology
Record Anti-Slavery Reporter p 425 1842 Jan 17. , notice quoted from the of the British and Foreign Anti-Slavery Society's address ...
1842 - Gale Group | SAS Part 1
Debra Ellisor, Dorothy Koveal, Nellwyn Hagan, Ashly Brown, Mark Zervas,
... a loxP flanked Stop sequence, which precedes a reporter allele. Upon recombination, the resulting phenotyping allele is ‘turned on’ and then constitutively expressed. Thus, the reporter functions as a high fidelity genetic lineage tracer in vivo. Currently there is an array of reporter alleles that can be used in marking strategies, ... To assess the recombination/marking potential of the reporters, we utilized CreERT under the control of a ... knock-in line in combination with three different reporters: R26R (LacZ reporter), Z/EG (EGFP reporter), and ...
Tópico(s): RNA Research and Splicing
2009 - Elsevier BV | Gene Expression Patterns
Project Gutenberg
Anti-Slavery Reporter Morning Herald p 122 1842 June 29. Extract from the explaining the circumstances relating to the insertion in ...
1842 - Gale Group | SAS Part 1
Judith McIntosh White, Tracy Rutherford,
... spongiform encephalopathy (BSE) event to discover impact of reporters' work role identities on news story source choices ... were divided into two equal groups based on reporters' work-role identity (dichotomized between science-specialty beat reporters and non-specialty reporters) and analyzed by length, number of sources, and ... sources for stories written by science-specialty beat reporters and those written by non-specialty reporters. However, while mean overall source variety did not differ between the two reporter groups, work-role identify correlated with use of ...
Tópico(s): Climate Change Communication and Perception
2009 - New Prairie Press | Journal of Applied Communications
Anti-Slavery Reporter pp 22 a-h 1835. , No. 112, pp 265-80, articles on British Guiana, Trinidad and Jamaica.
1835 - Gale Group | SAS Part 1
Jian Fu, Jirong Zhang, Fang Jin, Jamie Patchefsky, Karl‐Heinz Braunewell, Andres J. Klein–Szanto,
... pGL4.10[luc2] vector and pGL4.73 luciferase reporter vectors (Promega, Madison, WI) were used for VSNL1 promoter reporter assays. VP-1998 (VP2kb) was constructed as described ( ... 2000 (Invitrogen) using the manufacturer's protocol. For reporter gene assay, DNA mixture containing 0.8 μg ... step with 150 μl of passive lysis buffer. Reporter gene activity was measured in 96-well plates according to the protocol from Dual-Luciferase reporter 1000 assay system kit (Promega) by using an ... and 50 pmol was finally used in the reporter gene assay with 0.8 μg of VP- ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2009 - Elsevier BV | Journal of Biological Chemistry
Anti-Slavery Reporter, pp 33-36 [1835]. No. 112, article entitled Further Postscript quoting the speech of the Marquis of Sligo ...
1835 - Gale Group | SAS Part 1
Christian Kühn, Derk Frank, Rainer Will, Christoph Jaschinski, Robert Frauen, Hugo A. Katus, Norbert Frey,
... to robust induction of an NFAT-dependent luciferase reporter, whereas DYRK1A attenuated calcineurin-induced reporter activation in cardiomyocytes. Conversely, both knockdown and pharmacological ... to robust induction of an NFAT-dependent luciferase reporter, whereas DYRK1A attenuated calcineurin-induced reporter activation in cardiomyocytes. Conversely, both knockdown and pharmacological ... 24 h. Simultaneous to the infection with the reporter virus, cells were infected by adenoviruses as indicated. ... which had been tested to not activate the reporter. Luciferase assays were performed according to the manufacturer' ...
Tópico(s): Cardiac electrophysiology and arrhythmias
2009 - Elsevier BV | Journal of Biological Chemistry
Anti-Slavery Reporter pp 120-27 1835 Oct. Buxton to Lord Sligo, about his letters to Lord Suffield, his complaints of ...
1835 - Gale Group | SAS Part 1
Renata Usaite, Michael C. Jewett, Ana Paula Oliveira, John R. Yates, Lisbeth Olsson, Jens Nielsen,
... response to the loss of Snf1 kinase activity. Reporter effector analysis identified transcription factors (TFs), whose target ... a group to genetic Snf1 kinase complex disruptions. Reporter metabolite analysis identified metabolic hot spots that significantly ... 1). These four methods allowed us to identify reporter effectors reporter metabolites, high scoring sub-networks, and high scoring ... the loss of Snf1 kinase activity. (C) Represents Reporter Effector analysis tool (Oliveira et al, 2008) that, ... 3, Gene 4, and Gene 5. (D) Represents Reporter Metabolite Analysis (Patil and Nielsen, 2005) that, based ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2009 - Springer Nature | Molecular Systems Biology
Directory of Open Access Books (DOAB)
Anti-Slavery Reporter Mirror of Parliament pp 306-09 1836 Jan 12. Buxton to Paget, advising him to consult the , the ...
1836 - Gale Group | SAS Part 1
K. Arumugam, Yiying Wang, Linda Hardy, Melanie C MacNicol, Angus M. MacNicol,
... consensus polyadenylation hexanucleotides (grey hexagon). (B) β-globin reporter 3′ UTRs were generated by inserting a consensus ... of oocytes were injected with the indicated mRNA reporter constructs, incubated for approximately 16 h and then ... immature oocyte samples. The polyadenylation status of the reporter mRNAs analyzed by RNA-ligation coupled PCR using ... 2002). When examined in the context of a reporter mRNA, an MBE directs early polyadenylation (before GVBD), ... translation activation of cyclin B5 mRNA, we utilized reporter mRNAs fused to either the wild-type cyclin ...
Tópico(s): Genomics and Chromatin Dynamics
2009 - Springer Nature | The EMBO Journal
Anti-Slavery Reporter pp 448-49 1840 Oct 26. Buxton to Sir George Stephen, asking him to assist Washington in preparing ...
1840 - Gale Group | SAS Part 1
Seung‐Hee Nam, Kyoengwoo Min, Hye‐Jin Hwang, Hae-ock Lee, Jung Hwa Lee, Jong‐Bok Yoon, Hyun‐Sook Lee, Sungsu Park, Junho Lee,
... rapsyn stability control. We show that a functional reporter of muscular C. elegans RPY-1 is degraded ... National Health Institutes, Bethesda, MD). Construction of the Reporter Genes and rpy-1 Derivatives-For examination of rpy-1 expression patterns, we constructed a reporter gene that encoded the full-length C. elegans ... gene fused to GFP and Discosoma sp. red reporter genes. To ensure avoidance of mosaicism, which is ... isoform (NM_032645) were fused to the GFP reporter gene under the control of a muscle-specific ...
Tópico(s): ATP Synthase and ATPases Research
2009 - Elsevier BV | Journal of Biological Chemistry
Frontmatter: Notice to Subscribers of the West African Reporter, All Moneys and Post Office Orders Must …, The West African Reporter. Classified ads: Multiple Classified Advertisements, For Sherbro. News: ...
1884 - Gale Group | NCCO EuropeAfrica
Satoru Torii, Kentaro Kobayashi, Masayuki Takahashi, Kasumi Katahira, Kenji Goryo, Natsuki Matsushita, Ken‐ichi Yasumoto, Yoshiaki Fujii‐Kuriyama, Kazuhiro Sogawa,
... 2000 (Invitrogen). Construction of the HRE-dependent luciferase reporter plasmid and the assay of luciferase activity were ... of the E1b-luc2 plasmid. The dual luciferase reporter assay was performed according to the manufacturer's ... cells in different nutritional conditions, we introduced a reporter plasmid containing HREs (Fig. 1A) (17.Sogawa K. ... cultured PC12 cells, a 4-fold induction of reporter activity was found in response to CoCl2 (Fig. ... of grouped nutrients to RPMI and assayed the reporter activity (supplemental Fig. S1). Subsequently, each nutrient in ...
Tópico(s): Chronic Obstructive Pulmonary Disease (COPD) Research
2009 - Elsevier BV | Journal of Biological Chemistry