Prachi Korgaonkar, Ashish Patil, Nilesh Khochare,
... IGMP), Internet Protocol (IP), Address Resolution Protocol (ARP), Reverse Address Resolution Protocol(RARP), etc. Each Protocol has its own vulnerability, ...
Tópico(s): Advanced Malware Detection Techniques
2012 - | International journal of advanced research in computer science and electronics engineering
Darja Pollpeter, Andrew Sobala, Michael H. Malim,
... cell proteins on viral DNA synthesis. Here we address the lack of a cell-based, high-coverage, and high-resolution assay that is capable of defining retroviral reverse transcription intermediates within the physiological context of virus infection. The described method captures the 3'-termini of nascent complementary DNA (cDNA) molecules within HIV-1 infected cells at single nucleotide resolution. The protocol involves harvesting of whole cell DNA, targeted enrichment ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - MyJOVE | Journal of Visualized Experiments
Darja Pollpeter, Andrew Sobala, Michael H. Malim,
... cell proteins on viral DNA synthesis. Here we address the lack of a cell-based, high-coverage, and high-resolution assay that is capable of defining retroviral reverse transcription intermediates within the physiological context of virus infection. The described method captures the 3'-termini of nascent complementary DNA (cDNA) molecules within HIV-1 infected cells at single nucleotide resolution. The protocol involves harvesting of whole cell DNA, targeted enrichment ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - MyJOVE | Journal of Visualized Experiments
Ronald J. Bonnstetter, Eric Gehrig, Dustin Hebets,
... items. This pilot triangulation process is designed to address response process validation, as described by The American Psychological Association to begin to better understand whether the responder is following the intended purpose of the assessment. Method: The protocol involves collecting electroencephalographic (EEG) data, using standardized low-resolution brain electromagnetic tomography (sLORETA) to analyze and view ...
Tópico(s): Academic and Historical Perspectives in Psychology
2018 - International Society for Neurofeedback and Research (ISNR) | NeuroRegulation
John C. Greenwood, Fatima M. Talebi, David H. Jang, Audrey E. Spelde, Todd J. Kilbaugh, Frances S. Shofer, Michael A. Acker, John Augoustides, Jan Bakker, Nuala J. Meyer, Jacob S. Brenner, Vladimir R. Muzykantov, Benjamin S. Abella,
... measured by performing a substrate–uncoupler–inhibitor titration protocol with high resolution respirometry on peripheral blood mononuclear cells at baseline ...
Tópico(s): Cardiac, Anesthesia and Surgical Outcomes
2022 - Public Library of Science | PLoS ONE
Wei Yang, Nengquan Li, Jingsheng Miao, Lisi Zhan, Shaolong Gong, Zhongyan Huang, Chuluo Yang,
... which was conveniently synthesized by a two-step protocol. Expectedly, the resolution of helicene-BN afforded two helically chiral enantiomers, ...
Tópico(s): Photochromic and Fluorescence Chemistry
2022 - Chinese Chemical Society | CCS Chemistry
Batuhan Yenilmez, Mark Kelly, Guo‐Fang Zhang, Nicole Wetoska, Olga Ilkayeva, Kyounghee Min, Leslie A. Rowland, Chloe DiMarzio, Wentao He, Naideline Raymond, Lawrence M. Lifshitz, Meixia Pan, Xianlin Han, Jun Xie, Randall H. Friedline, Jason K. Kim, Guangping Gao, Mark A. Herman, Christopher B. Newgard, Michael Czech,
... both are depleted. Figure 2A depicts the experimental protocol used to address the key questions of our study concerning regulation ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2022 - Elsevier BV | Journal of Biological Chemistry
Hao Wu, Benjamin D. Humphreys,
... RNA. Only 10% to 20% of this is reverse-transcribed regardless of the scRNA-seq protocol, and, as a consequence, all protocols use an ...
Tópico(s): Renal and related cancers
2017 - Elsevier BV | Kidney International
Yajing Zhang, Fangfang Liu, Chengli Zhang, Meijing Ren, Manchao Kuang, Ting Xiao, Xuebing Di, Lin Feng, Li Fu, Shujun Cheng,
... MA) and reverse transcribed using a SuperScript II reverse transcription kit according to the manufacturer's protocol (catalog number 18064014; Thermo Fisher Scientific). Then real- ...
Tópico(s): Cancer-related Molecular Pathways
2019 - Elsevier BV | American Journal Of Pathology
Jill M. Hoffman, Aristea Sideri, Jonathan J. Ruiz, Dimitris Stavrakis, David Q. Shih, Jerrold R. Turner, Charalabos Pothoulakis, Ιορδάνης Καραγιαννίδης,
... and mouse colonic tissue using standard Trizol-based protocols, and complementary DNA was generated by reverse-transcriptase (RT) reaction (Promega, Madison, WI). An Applied ...
Tópico(s): Infant Nutrition and Health
2018 - Elsevier BV | Cellular and Molecular Gastroenterology and Hepatology
Britta A. M. Bouwman, Nicola Crosetto, Magda Bienko,
... an emerging approach that can be leveraged to address fundamental research questions on intercell genome variability and for cancer diagnostics, allowing high-resolution mapping of intratumor heterogeneity.Together, spatial genomics and ... 502: 59-64Crossref PubMed Scopus (906) Google Scholar] protocol became available to generate genome-wide chromatin contact maps of single cells. To reduce loss of material and increase resolution, more recent approaches such as Dip-C [49. ... 502: 59-64Crossref PubMed Scopus (906) Google Scholar] protocol became available to generate genome-wide chromatin contact maps of single cells. To reduce loss of material and increase resolution, more recent approaches such as Dip-C [49. ...
Tópico(s): Genomic variations and chromosomal abnormalities
2022 - Elsevier BV | Trends in Genetics
Thorsten Derlin, Benedikt Jaeger, Danny Jonigk, Rosa Marie Apel, Julia Freise, Hoen‐oh Shin, Desiree Weiberg, G. Warnecke, Tobias L. Roß, Hans‐Jürgen Wester, Benjamin Seeliger, Tobias Welte, Frank M. Bengel, Antje Prasse,
... no. 74104; Qiagen) in accordance with the standard protocol provided by the manufacturer. Total RNA were reverse transcribed to complementary DNA by using the High- ...
Tópico(s): Sarcoidosis and Beryllium Toxicity Research
2020 - Elsevier BV | CHEST Journal
Marc Hammarlund, Oliver Hobert, David M. Miller, Nenad Šestan,
... the neuronal transcriptome from animal to animal? To address these questions, CeNGEN will also use single-cell-sequencing technology (scRNA-seq) to provide single-neuron resolution at selected developmental stages. The bulk sequencing data ...
Tópico(s): Bioinformatics and Genomic Networks
2018 - Cell Press | Neuron
Elliott D. Crouser, Michael A. Matthay,
... achieve hemodynamic stability. More fundamental therapies that can reverse endothelial injury may be needed. It is also possible that restoration of macroscopic blood flow, such as is achieved by EGDT, fails to address some of the critical mechanisms of organ damage. ...
Tópico(s): Respiratory Support and Mechanisms
2017 - Elsevier BV | CHEST Journal
Produção Nacional
Revisado por pares
Marie Fidela R. Paraiso, Jubilee Brown, Maurício Simões Abrão, H Dionisi, R.B. Rosenfield, Ted T.M. Lee, Nucelio Lemos,
... based on institutional COVID-19 diagnostic and treatment protocols. Surgical rescheduling should require clinical improvement, normalization of chest CT scans, and 2 negative RT-PCR tests to confirm resolution [31Scheib S. Covid-19: impact in New Orleans ...
Tópico(s): Cardiac, Anesthesia and Surgical Outcomes
2020 - Elsevier BV | Journal of Minimally Invasive Gynecology
Hajime Ogino, Yasuhiro Kamei, Toshinori Hayashi, Joe Sakamoto, Makoto Suzuki, Takeshi Igawa,
... may change his words, if the "Methods and Protocols" section could help the developmental biologists to address questions such as “Why is the body size ...
Tópico(s): Pluripotent Stem Cells Research
2021 - Wiley | Development Growth & Differentiation
Tatiana Leonova, Christian Ihling, Mohamad Saoud, Nadezhda Frolova, Robert C. Rennert, Ludger A. Wessjohann, Andrej Frolov,
... for plant matrices. To fill this gap, we address here the potential of FASP in combination with two protein isolation protocols for quantitative analysis of pea ( Pisum sativum ) seed ...
Tópico(s): Identification and Quantification in Food
2022 - Frontiers Media | Frontiers in Plant Science
Veit Schwämmle, Christina E. Hagensen, Adelina Rogowska‐Wrzesinska, Ole N. Jensen,
... scale proteomics results, regardless of instrument platform, experimental protocol and software tools. However, the multitude of different combinations of experimental strategies, mass spectrometry techniques and informatics methods complicate the decision of choosing appropriate statistical approaches. We address this challenge by introducing PolySTest, a user-friendly ... scale proteomics results, regardless of instrument platform, experimental protocol and software tools. However, the multitude of different combinations of experimental strategies, mass spectrometry techniques and informatics methods complicate the decision of choosing appropriate statistical approaches. We address this challenge by introducing PolySTest, a user-friendly ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2020 - Elsevier BV | Molecular & Cellular Proteomics
Leslie Chang, Lei Zhang, Jiajia Xu, Carolyn A. Meyers, Li Zhu, Noah Yan, Erin Zou, Aaron W. James,
... 5′-GAACCTGATGGACATGTTCAGG-3′ (forward) and 5′-AGTGCGTTCGAACGCTAGAGCCTGT-3′ (reverse). All animal experiments were performed according to the approved protocol of the Animal Care and Use Committee at ... and the animal was monitored per established postoperative protocols. Animals were sacrificed at 3, 7, 14, and 21 days after surgery for analysis (n = 3 mice per time point). Samples were harvested from 3 to 21 days after injury, fixed in 4% paraformaldehyde for 24 hours, and scanned using a high-resolution micro–computed tomography imaging system (SkyScan 1294; Bruker ...
Tópico(s): Knee injuries and reconstruction techniques
2018 - Elsevier BV | American Journal Of Pathology
Yunjie Tu, Li He, Tao Tu, Qi Zhang,
... Mineralization: A Biocatalyst for Ester Hydrolysis and Kinetic Resolution.ACS Appl. Mater. Interfaces2016, 8, 24517–24524. Google Scholar 29. Chulkaivalsucharit P.; Wu X.; Ge J.Synthesis of Enzyme-Embedded Metal–Organic Framework Nanocrystals in Reverse Micelles.RSC Adv.2015, 5, 101293–101296. Google ...
Tópico(s): Dendrimers and Hyperbranched Polymers
2021 - Chinese Chemical Society | CCS Chemistry
Chuan‐Chih Hsu, Yingfang Zhu, Justine Arrington, Sebastian Juan Paez, Pengcheng Wang, Peipei Zhu, I‐Hsuan Chen, Jian‐Kang Zhu, W. Andy Tao,
... 1168-1182Crossref PubMed Scopus (41) Google Scholar). To address the limits of global plant phosphoproteomics, we have carefully evaluated the performance of several techniques that have been previously employed in each proteomic sample preparation step. We introduce here a universal sample preparation protocol, which significantly increased the coverage and depth of ... was used for reverse transcription using M-MLV reverse transcriptase (Promega). Real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA) on a CFX96 real-time PCR detection system (Bio-Rad). Tomato Actin 7 (ACT7) was used as the internal reference for all reactions. Ground tomato leaves were lysed in (1) Tris-HCl protocol: 100 mm Tris-HCl (pH 7.5), 250 ...
Tópico(s): Plant nutrient uptake and metabolism
2018 - Elsevier BV | Molecular & Cellular Proteomics
Dana Boyd Barr, Naveen Puttaswamy, Lindsay M. Jaacks, Kyle Steenland, Sarah Rajkumar, Savannah Gupton, P. Barry Ryan, Kalpana Balakrishnan, Jennifer L. Peel, William Checkley, Thomas Clasen, Maggie L. Clark,
... HAPIN, Household Air Pollution Intervention Network; HRMS, high resolution mass spectrometry; ICP-MS, inductively coupled plasma-mass spectrometry; LC-MS/MS, liquid chromatography-tandem mass spectrometry; NT, nasal turbinate swab; POC, point-of-care; RT-PCR, reverse transcription–polymerase chain reaction.aMeasured in all other ...
Tópico(s): Climate Change and Health Impacts
2020 - National Institute of Environmental Health Sciences | Environmental Health Perspectives
Junichi Matsumoto, Shingo Takada, Shintaro Kinugawa, Takaaki Furihata, Hideo Nambu, Naoya Kakutani, Masaya Tsuda, Arata Fukushima, Takashi Yokota, Shinya Tanaka, Hidehisa Takahashi, Masashi Watanabe, Shigetsugu Hatakeyama, Masaki Matsumoto, Keiichi I. Nakayama, Yutaro Otsuka, Hisataka Sabe, Hiroyuki Tsutsui, Toshihisa Anzai,
... with the sham mice as measured by high-resolution respirometry (Oxygraph-2k, Oroboros Instruments; Figure [C]).Download figureDownload PowerPointFigure. Brain-derived neurotrophic factor (BDNF) improves limited exercise capacity and mitochondrial respiration in skeletal muscle from heart failure mice.A, Study protocol 1 (at 2 weeks after surgery in sham ...
Tópico(s): Neuroscience and Neural Engineering
2018 - Lippincott Williams & Wilkins | Circulation
Nikhil Sahajpal, Ashis K. Mondal, Allan Njau, Sudha Ananth, S. Ghamande, Madhuri Hegde, Alka Chaubey, Amyn M. Rojiani, Ravindra Kolhe,
... investigation in larger cohorts. Well-defined sample collection protocols should help resolve some of the discrepancies reported in the literature but concordant results observed with multiple studies in healthcare setting endorse the clinical utility of saliva samples in healthcare setting. Resolution of specimen collection variability could result in implementation ...
Tópico(s): SARS-CoV-2 and COVID-19 Research
2020 - Future Science Ltd | Future Medicinal Chemistry
Cheng-Te Hsiao, Po-Wei Wang, Hua-Chien Chang, Yen‐Ying Chen, Shuihua Wang, Yijuang Chern, Kay‐Hooi Khoo,
... processed automatically by GlyPick. The high acquisition speed, resolution, and mass accuracy afforded by top-notch Orbitrap Fusion MS system now allow a sensible spectral count and/or summed ion intensity-based glycome-wide glycotope quantification. We report here the technical aspects, reproducibility and optimization of such an aNAlytical approach that uses the same acidic reverse phase C18 NAnoLC conditions fully compatible with proteomic ... processed automatically by GlyPick. The high acquisition speed, resolution, and mass accuracy afforded by top-notch Orbitrap Fusion MS system now allow a sensible spectral count and/or summed ion intensity-based glycome-wide glycotope quantification. We report here the technical aspects, reproducibility and optimization of such an aNAlytical approach that uses the same acidic reverse phase C18 NAnoLC conditions fully compatible with proteomic ...
Tópico(s): Genomics and Phylogenetic Studies
2017 - Elsevier BV | Molecular & Cellular Proteomics
Bobby Yanagawa, Subodh Verma, C. David Mazer,
... found this link.16, 17 Further studies with protocol‐driven cerebral imaging will be needed to better understand the cerebral embolic risk. Other important questions to address include whether subclinical valve leaflet thrombosis occurs in ...
Tópico(s): Cardiac Structural Anomalies and Repair
2017 - Wiley | Journal of the American Heart Association
Evgeny Kanshin, Sébastien Giguère, Jing Cheng, Mike Tyers, Pierre Thibault,
... Each sample was loaded and separated on a reverse-phase analytical column (18 cm length, 150 μm inner diameter) (Jupiter C18, 3 μm, 300 Å, Phenomenex) packed manually. LC separations were performed at a flow rate of 0.6 μl/min using a linear gradient of 5–30% aqueous acetonitrile (0.2% FA) in 106 min. MS spectra were acquired with a resolution of 60,000. The TopSpeed (maximum number of ...
Tópico(s): Fungal and yeast genetics research
2017 - Elsevier BV | Molecular & Cellular Proteomics
Gretchen LeFever Watson, Nancy Selfridge, Bruce E. Wright,
... improvement by establishing clear policy and an associated protocol containing highly scripted language (see Table 2). Research has shown that the practice of full disclosure can substantially decrease the number of claims, lawsuits, and time from reporting to resolution as well as liability, compensation, and administrative costs.34 Full disclosure also guards against the costly second victim phenomenon “whereby health care workers are also traumatized by the same events that harm patients.”35 The success of this well-coordinated effort to address one of healthcare's thorniest issues—an issue ...
Tópico(s): Healthcare professionals’ stress and burnout
2020 - Wiley | Health Science Reports
Liza O’Donnell, Diane Rebourcet, Laura F. Dagley, Raouda Sgaier, Giuseppe Infusini, Peter J. O’Shaughnessy, Frédéric Chalmel, Daniela Fietz, W. Weidner, Julien M. D. Legrand, Robin M. Hobbs, Robert I. McLachlan, Andrew I. Webb, Adrian Pilatz, Thorsten Diemer, Lee B. Smith, Peter G. Stanton,
... induced dissociation (CID). Raw files consisting of high-resolution MS/MS spectra were processed with MaxQuant (version 1.5.8.3) for feature detection and protein identification using the Andromeda search engine.32 Extracted peak lists were searched against the UniProtKB/Swiss-Prot Mus musculus or Homo sapiens databases (October 2016) and a separate reverse decoy database to empirically assess the false discovery ...
Tópico(s): Xenotransplantation and immune response
2021 - Wiley | The FASEB Journal
Lin Zhu, Sin‐Yee Fung, Guangshan Xie, Lok-Yin Roy Wong, Dong‐Yan Jin, Zongwei Cai,
... were loaded on a self-pack C18 analytical reverse phase column (ID 75 μm × 15 cm, 200Å, 3 μm particles) at flow rate of 300 nL/min and a 75 min LC gradient of 8% to 28% ACN in 0.1% FA was used. Orbitrap fusion tribrid (Thermo-Fisher) MS machine was used to analyze the peptide sample in a data-dependent acquisition mode, with 120,000 resolution at MS1 scan and 30,000 at MS2 ( ...
Tópico(s): Virus-based gene therapy research
2020 - Elsevier BV | Molecular & Cellular Proteomics