Prachi Korgaonkar, Ashish Patil, Nilesh Khochare,
... IGMP), Internet Protocol (IP), Address Resolution Protocol (ARP), Reverse Address Resolution Protocol(RARP), etc. Each Protocol has its own vulnerability, ...
Tópico(s): Advanced Malware Detection Techniques
2012 - | International journal of advanced research in computer science and electronics engineering
Darja Pollpeter, Andrew Sobala, Michael H. Malim,
... cell proteins on viral DNA synthesis. Here we address the lack of a cell-based, high-coverage, and high-resolution assay that is capable of defining retroviral reverse transcription intermediates within the physiological context of virus infection. The described method captures the 3'-termini of nascent complementary DNA (cDNA) molecules within HIV-1 infected cells at single nucleotide resolution. The protocol involves harvesting of whole cell DNA, targeted enrichment ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - MyJOVE | Journal of Visualized Experiments
Darja Pollpeter, Andrew Sobala, Michael H. Malim,
... cell proteins on viral DNA synthesis. Here we address the lack of a cell-based, high-coverage, and high-resolution assay that is capable of defining retroviral reverse transcription intermediates within the physiological context of virus infection. The described method captures the 3'-termini of nascent complementary DNA (cDNA) molecules within HIV-1 infected cells at single nucleotide resolution. The protocol involves harvesting of whole cell DNA, targeted enrichment ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - MyJOVE | Journal of Visualized Experiments
... debate on S. Res. 98., the Byrd-Hagel Resolution, Cong. Rec. S8115, 25 July 1997. In 2009, efforts to address global climate change are underway in a wide range of forums. Parties to the United Nations Framework Convention on Climate Change (UNFCCC) and its Kyoto Protocol aim to adopt, in Copenhagen, Denmark in December ...
Tópico(s): Global Energy and Sustainability Research
2009 - Oxford University Press | Duke journal of comparative & international law
Robin Andersson, Yun Chen, Leighton J. Core, John T. Lis, Albin Sandelin, Torben Heick Jensen,
... due to differences in the published GRO-seq protocols (see Supplemental Experimental Procedures), rather than because of differently used thresholds. What then is the degree of divergent transcription initiation from human gene promoters? To address this, we assessed which fraction of transcribed gene promoters analyzed by Duttke et al. also display detectable reverse-strand transcription as a function of thresholds on ...
Tópico(s): Gene Regulatory Network Analysis
2015 - Elsevier BV | Molecular Cell
Mark Sevecka, Alejandro Wolf‐Yadlin, Gavin MacBeath,
... Overall, our list of validated antibodies and associated protocols establish lysate microarrays as a robust tool for systems biology. Lysate microarrays (reverse-phase protein arrays) hold great promise as a tool for systems-level investigations of signaling and multiplexed analyses of disease biomarkers. To date, however, widespread use of this technology has been limited by questions concerning data quality and the specificity of detection reagents. To address these concerns, we developed a strategy to identify ...
Tópico(s): Gene expression and cancer classification
2011 - Elsevier BV | Molecular & Cellular Proteomics
Kirsten L Coffey, A.V. Krushinsky, Colin Green, Paul J. Donaldson,
... cell layers has not been comprehensively studied. To address this issue the authors have performed an extensive molecular screening of connexin (Cx) expression patterns in ciliary epithelium of the rat. Initially, mRNA was extracted from rat ciliary bodies, reverse-transcribed, and subjected to two rounds of PCR using primer sets designed against each of the 14 Cx isoforms known to be expressed in the rat. This initial screening protocol amplified eight candidate Cx isoforms (Cxs 26, 31, ...
Tópico(s): Biochemical effects in animals
2002 - Elsevier BV | Experimental Eye Research
Alexander Y. Andreyev, Zhouxin Shen, Ziqiang Guan, Andrea C. Ryan, Eoin Fahy, Shankar Subramaniam, Christian R.H. Raetz, Steven P. Briggs, Edward A. Dennis,
... ammonium acetate for 20 min) followed by high resolution reverse phase separation using an acetonitrile gradient of 0– ...
Tópico(s): Mitochondrial Function and Pathology
2009 - Elsevier BV | Molecular & Cellular Proteomics
Cláudia Piccoli, Roberto Ria, Rosella Scrima, Olga Cela, Annamaria D’Aprile, Domenico Boffoli, Franca Falzetti, Antonio Tabilio, Nazzareno Capitanio,
... the legend of Fig. 3D) following the SuperScript Reverse Transcriptase protocol. Samples of 5 μl of reverse transcription reaction were PCR-amplified in a total ...
Tópico(s): Neutrophil, Myeloperoxidase and Oxidative Mechanisms
2005 - Elsevier BV | Journal of Biological Chemistry
Heeyoung Jung, Wan‐Seon Lim, Jungha Hong, Cinyoung Hur, Joochul Lee, Taewan You, Jee-Sook Eun, B.-J. Kwak, J.H. Kim, Hae Sook Jeon, Taewhan KIM, Woojik Chun,
... ID (EID) from Routing Locators (RLOC) to mainly address board gateway protocol (BGP) routing scalability. In LISP, both EID and ... and the map-reply message then takes the reverse path. Figure 3Open in figure viewerPowerPoint ILMS structure. To address the scalability on the ever-increasing number of ...
Tópico(s): Software-Defined Networks and 5G
2015 - Electronics and Telecommunications Research Institute | ETRI Journal
Min Cong, Tianhui Liu, Ping Wang, Fan Xu, Aiting Yang, Yanfeng Bai, Zhenwei Peng, Peng Wu, Xiaofei Tong, Jing Chen, Hai Li, Rui Cong, Shuzhen Tang, Bao-en Wang, Ji-dong Jia, Hong You,
... Carlsbad, CA) extraction according to the manufacturer’s protocol. The reverse transcription reaction was performed using a reverse transcription ...
Tópico(s): Liver Disease Diagnosis and Treatment
2013 - Elsevier BV | American Journal Of Pathology
Alison A. Gardner, Ethan C. Reichert, Matthew K. Topham, Diana M. Stafforini,
... it was necessary to adapt the standard fractionation protocol to improve resolution in the heavy density region. We adjusted the ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
2008 - Elsevier BV | Journal of Biological Chemistry
... WTO agreements on one hand, and an accession protocol whose provisions modify the application of the WTO agreements to the acceded Member, on the other. In addition, the Appellate Body chose not to address the dissenting opinion from the Panel below and the views of several third parties that disagreed with its prior ruling on the same legal issue in China-Raw Materials, thereby forgoing the opportunity to reexamine and possibly reverse that problematic ruling. The Appellate Body’s decision ...
Tópico(s): International Arbitration and Investment Law
2014 - RELX Group (Netherlands) | SSRN Electronic Journal
... and N.W. Ross. 1995. A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide ...
Tópico(s): RNA and protein synthesis mechanisms
2006 - Future Science Ltd | BioTechniques
Nicolas Poulalhon, Dominique Farge, N. Roos, Charlotte Tacheau, Cindy Neuzillet, Laurence Michel, Alain Mauviel, Franck Verrecchia,
... an MTS assay according to the manufacturer's protocol (Promega, Madison, WI). Reverse Transcription-PCR Analysis—Total RNA was extracted according ...
Tópico(s): Protease and Inhibitor Mechanisms
2006 - Elsevier BV | Journal of Biological Chemistry
Kikuo Isoda, Shojiro Sawada, Makoto Ayaori, Taizo Matsuki, Reiko Horai, Yutaka Kagata, Kôji Miyazaki, Masatoshi Kusuhara, Mitsuyo Okazaki, Osamu Matsubara, Yoichiro Iwakura, Fumitaka Ohsuzu,
... to the manufacturer's instructions. Purified RNA was reverse-transcribed according to the protocols supplied by the manufacturer. Quantitative gene expression analysis ...
Tópico(s): Liver Disease Diagnosis and Treatment
2004 - Elsevier BV | Journal of Biological Chemistry
Henna M. Moore, Baoyan Bai, François‐Michel Boisvert, Leena Latonen, Ville Rantanen, Jeremy C. Simpson, Rainer Pepperkok, Angus I. Lamond, Marikki Laiho,
... in Leica EMstain automatic staining unit under standard protocols. Uranyl acetate and lead citrate increase resolution and contrast of cellular structures, such as nucleoli ...
Tópico(s): RNA and protein synthesis mechanisms
2011 - Elsevier BV | Molecular & Cellular Proteomics
Beatriz Sánchez-Vega, Francisco Vega, L. Jeffrey Medeiros, Ming S. Lee, Rajyalakshmi Luthra,
... and post-treatment specimens from a patient. To address these limitations, we designed a multiplex real-time PCR protocol that allows amplification of control and target genes ... and post-treatment specimens from a patient. To address these limitations, we designed a multiplex real-time PCR protocol that allows amplification of control and target genes ...
Tópico(s): T-cell and Retrovirus Studies
2002 - Elsevier BV | Journal of Molecular Diagnostics
Lanlan Shen, Yi Guo, Han Y. H. Chen, Saira Ahmed, Jean‐Pierre J. Issa,
... contentAnalytical Biochemistry, Vol. 384, No. 2Methylation-sensitive high-resolution melting20 November 2008 | Nature Protocols, Vol. 3, No. 12Variable DNA methylation patterns associated ...
Tópico(s): Genomics and Chromatin Dynamics
2007 - Future Science Ltd | BioTechniques
Yi-Nan Zhang, Ehtesham Baig, David B. Williams,
... of random primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to the manufacturer's protocol. For quantitative PCR, reaction components were obtained from ...
Tópico(s): Heat shock proteins research
2006 - Elsevier BV | Journal of Biological Chemistry
Saša Štefanić, Cornelia Spycher, Laura Morf, Gemma Fabriàs, Josefina Casas, Elisabeth M. Schraner, Peter J. Wild, Adrian B. Hehl, Sabrina Sonda,
... was performed using ∼350 ng RNA and Omniscript reverse transcriptase (Qiagen), according to manufacturer's protocol. Amplification was performed in an iCycler iQ (Biorad, ...
Tópico(s): Toxoplasma gondii Research Studies
2010 - Elsevier BV | Journal of Lipid Research
J. W. Head, Tanya R. Mealy, Francis X. McCormack, Barbara A. Seaton,
... was then further refined to 2.1-Å resolution against the native data using simulated annealing protocols. Iterative cycles of rebuilding and refinement, using difference Fourier and simulated annealing omit maps, were used to produce the final model. Broken density at the N terminus precluded unambiguous positioning of residues 81–83, which were eventually omitted. For the structure of the SP-A/samarium complex, 2.5-Å resolution data at the remote wavelength (λ = 1.771 ...
Tópico(s): Protein Structure and Dynamics
2003 - Elsevier BV | Journal of Biological Chemistry
Robert Walczak, Sean B. Joseph, Bryan Laffitte, Antonio Castrillo, Liming Pei, Peter Tontonoz,
... reverse transcribed with random hexamers using the Taqman reverse transcription reagent kit (Applied Biosystems) according to the manufacturer's protocol. Each Taqman reaction (50 μl) contained 50 ng of cDNA, 900 nm forward primer, 900 nm reverse primer, 100 nm dual-labeled fluorogenic probe (IDT), ...
Tópico(s): Cancer, Lipids, and Metabolism
2004 - Elsevier BV | Journal of Biological Chemistry
Alexander Heiss, Alexander DuChesne, Bernd Denecke, Joachim Grötzinger, Kazuhiko Yamamoto, Thomas Renné, Willi Jahnen‐Dechent,
... RNA was prepared from HepG2 cells following established protocols. The cystatin domains were cloned by reverse transcription-PCR using murine leukemia virus reverse transcriptase ( ...
Tópico(s): Blood properties and coagulation
2003 - Elsevier BV | Journal of Biological Chemistry
Jérôme N. Feige, Laurent Gelman, Cicerone Tudor, Yves Engelborghs, Walter Wahli, Béatrice Desvergne,
... analysis presented in Fig. 5 (recovery curves), the resolution was switched to 128 × 128 pixels, and forward-reverse scanning was applied allowing acquisition of images every ...
Tópico(s): Protein Degradation and Inhibitors
2005 - Elsevier BV | Journal of Biological Chemistry
Derek Pociask, Kong Chen, Sun Mi Choi, Tim D. Oury, Chad Steele, Jay K. Kolls,
... γδ T cells from CXCL10−/− mice failed to reverse the severe fibrosis in γδ KO mice. These results indicate that γδ T cells promote the resolution of fibrosis through the production of CXCL10. γδ ... γδ T cells from CXCL10−/− mice failed to reverse the severe fibrosis in γδ KO mice. These results indicate that γδ T cells promote the resolution of fibrosis through the production of CXCL10. Both ...
Tópico(s): Immune Cell Function and Interaction
2011 - Elsevier BV | American Journal Of Pathology
Ching‐Yu Lin, Huiming Huang, Han‐Min Chen,
... CAS, Google Scholar10. Fernandez-Patron, C. 2002. Zinc-reverse staining technique, p. 251–258. In J.M. Walker (Ed.), The Protein Protocols Handbook. Humana Press, Totowa.Crossref, Google Scholar11. Castellanos- ... gel electrophoresis by passive elution of lipopolysaccharides from reverse-stained gels: analysis of gel-purified lipopolysaccharides from Haemophilus influenzae strain Rd. Anal. Chem. 75:4918–4924.Crossref, Medline, CAS, Google Scholar14. Pupo, E., N.J. Phillips, B.W. Gibson, M.A. Apicella, and E. Hardy. 2004. Matrix-assisted laser desorption/ionization-time of flightmass spectrometry of lipopolysaccharide species separated by slab-polyacrylamide gel electrophoresis: high-resolution separation and molecular weight determination of lipooligosaccharides from ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2006 - Future Science Ltd | BioTechniques
Parameswari Govindarajan, Wolfgang Böcker, Thaqif El Khassawna, Marian Kampschulte, Gudrun Schlewitz, Britta Huerter, Ursula Sommer, Lutz Dürselen, Anita Ignatius, Natali Bauer, Gábor Szalay, Sabine Wenisch, Katrin Susanne Lips, Reinhard Schnettler, Alexander C. Langheinrich, Christian Heiß,
... Total RNA was extracted using TRIzol manufacturer’s protocol (Invitrogen, Carlsbad, CA). Briefly, 1 μg of RNA was reverse transcribed to cDNA with a QuantiTect Reverse Transcriptase Kit (Qiagen, Hilden, Germany). Real-time analysis was done with a Bio-Rad iQ5 PCR cycler (Bio-Rad, Paris, France) by a two-step cycling protocol using a Quantifast SYBR Green RT-PCR kit ( ...
Tópico(s): Bone Metabolism and Diseases
2014 - Elsevier BV | American Journal Of Pathology
Katrin Tanneberger, Juörgen Kirchberger, Joörg Baör, Wolfgang Schellenberger, Sven Rothemund, Manja Kamprad, Henning Otto, Torsten Schoöneberg, Anke Edelmann,
... Google Scholar) and according to the manufacturer's protocol (Gene-Racer™ kit with cloned avian myeloblastosis virus reverse transcriptase, Invitrogen). PCR fragments were subcloned into pCR2. ...
Tópico(s): Metabolism, Diabetes, and Cancer
2007 - Elsevier BV | Journal of Biological Chemistry
Sarah M. Tete, David Kipling, Johanna Westra, Aalzen de Haan, Marc Bijl, Deborah K. Dunn–Walters, Surinder S. Sahota, Nicolaas A. Bos,
... strand cDNA was synthesized from RNA following standard protocols using Superscript-III (Invitrogen, Waltham, MA) in a reverse transcriptase system (Promega, Madison, WI). cDNA was used ...
Tópico(s): Multiple Myeloma Research and Treatments
2015 - Elsevier BV | Experimental Hematology