A. R. Sanderson, William G. Juergens, Jack L. Strominger,
The β ribitol teichoic acid was extracted and purified from Staphylococcus aureus strain Wood 46 and chemically and immunologically characterised. Rabbit ... aureus cells. Liquid phase immunoprecipitation of the β ribitol teichoic acid-anti-Staphylococcus aureus serum system was ... nephelometry. Various mono- and disaccharides (N-acetyl-glucosamine-ribitol with α- or β-linkage and N-acetyl-glucosamine-ribitol-phosphate with β-linkage) were prepared by organic synthesis, reproducing part of the ribitol teichoic acid molecule. Inhibition by those mono- or ...
Tópico(s): Natural product bioactivities and synthesis
1961 - Elsevier BV | Biochemical and Biophysical Research Communications
Gunnar Haukenes, D. C. Ellwood, J. Baddiley, Per Oeding,
The β ribitol teichoic acid was extracted and purified from Staphylococcus aureus strain Wood 46 and chemically and immunologically characterised. Rabbit ... aureus cells. Liquid phase immunoprecipitation of the β ribitol teichoic acid-anti-Staphylococcus aureus serum system was ... nephelometry. Various mono- and disaccharides (N-acetyl-glucosamine-ribitol with α- or β-linkage and N-acetyl-glucosamine-ribitol-phosphate with β-linkage) were prepared by organic synthesis, reproducing part of the ribitol teichoic acid molecule. Inhibition by those mono- or ...
Tópico(s): Polysaccharides and Plant Cell Walls
1961 - Elsevier BV | Biochimica et Biophysica Acta
D.R.D. Shaw, David Mirelman, Anadi N. Chatterjee, James T. Park,
... lack N-acetylglucosamine (GlcNAc) on the cell wall ribitol teichoic acid, the CDP-ribitol:acceptor phosphoribitoltransferase was normal but no UDP-GlcNAc:poly(ribitol phosphate) GlcNAc-transferase activity was detected. Revertants of ... Type II mutants (52A5, 52A7, M3, M9) lacked ribitol teichoic acid in the cell wall and no ribitol phosphate polymer was detected in any other cell ... in the spent medium. Despite the lack of ribitol teichoic acid, normal activities in vitro of phosphoribitoltransferase ... type II mutants contained high levels of CDP-ribitol. The lack of ribitol teichoic acid in the ...
Tópico(s): Microbial Natural Products and Biosynthesis
1970 - Elsevier BV | Journal of Biological Chemistry
Theresa M. Bisson, Eugene J. Oliver, Robert P. Mortlock,
The incubation of Aerobacter aerogenes PRL-R3 with ribitol resulted in the induction of ribitol dehydrogenase and d -ribulokinase, coordinately controlled enzymes of the pathway of ribitol catabolism. A dehydrogenase-negative mutant was unable to induce d -ribulokinase activity following incubation with ribitol. Similar experiments using a kinase-negative mutant resulted in normal induction of ribitol dehydrogenase, as compared to the wild-type PRL- ... d -ribulose. In contrast to the experiments using ribitol as the substrate, the isomerization of d -arabinose ...
Tópico(s): Amino Acid Enzymes and Metabolism
1968 - American Society for Microbiology | Journal of Bacteriology
Smriti U. Mehta, Autar K. Mattoo, V. V. Modi,
1. Supplementation of cultures of Eremothecium ashbyii with ribitol leads to a twofold increase in riboflavin formation ... with ribose or ribulose phosphate. Addition of unlabelled ribitol decreases the incorporation of [1-(14)C]ribose into riboflavin, indicating that free ribitol is preferred to ribose for incorporation into riboflavin. 2. The enzymes ribitol kinase, d-ribose reductase, d-ribose 5'-phosphatase ... nucleosidase were demonstrated in the cell-free extracts. Ribitol induces the formation of ribitol kinase. The enzyme ...
Tópico(s): Diet, Metabolism, and Disease
1972 - Portland Press | Biochemical Journal
C. Kahle, K.-H. Schneider, Friedrich Giffhorn,
The phototrophic bacterium Rhodobacter sphaeroides strain Si4 induced ribitol dehydrogenase (EC 1.1.1.56) when grown on ribitol- or xylitol-containing medium. This ribitol dehydrogenase was purified to apparent homogeneity by ammonium ... r 25000 suggesting a dimeric enzyme structure. The ribitol dehydrogenase was specific for NAD+ but unspecific as ... its polyol substrate. In order of decreasing activity ribitol, xylitol, erythritol, D-glucitol and D-arabitol were ... 5. The equilibrium constant of the interconversion of ribitol to D-ribulose was determined to be 0· ...
Tópico(s): Aldose Reductase and Taurine
1992 - Microbiology Society | Journal of General Microbiology
Mark P. Pereira, Eric D. Brown,
CDP-ribitol synthase catalyzes the formation of CDP-ribitol from ribulose 5-phosphate, NADPH, and CTP. CDP-ribitol is an activated precursor for the synthesis of ... including Staphylococcus aureus. We showed previously that CDP-ribitol synthase activity in H. influenzae is catalyzed by ... the work reported here, we predicted a CDP-ribitol synthesis locus in S. aureus tandemly arranged as ... have shown the formation of a functional CDP-ribitol synthase complex between TarI and TarJ. Steady-state mechanistic studies of the CDP-ribitol synthases TarIJ and Bcs1 revealed that the analogous ...
Tópico(s): Polyamine Metabolism and Applications
2004 - American Chemical Society | Biochemistry
Alexa Bourand, Marı́a J. Yebra, Grégory Boël, Alain Mazé, Josef Deutscher,
... not ATCC 334, are able to ferment D-ribitol (also called D-adonitol). However, a BL23-derived ... suggesting that strain BL23 transports and phosphorylates D-ribitol via a PTS. We identified an 11-kb ... and six metabolic enzymes potentially involved in D-ribitol metabolism. Deletion of the gene encoding the EIIB component of the presumed ribitol PTS indeed prevented D-ribitol fermentation. In addition, we overexpressed the six catabolic ... of four of them. They encode a D-ribitol-5-phosphate (D-ribitol-5-P) 2-dehydrogenase, ...
Tópico(s): Polyamine Metabolism and Applications
2013 - American Society for Microbiology | Journal of Bacteriology
Osao Adachi, Yoshikazu Fujii, Yoshitaka Ano, Duangtip Moonmangmee, Hirohide Toyama, Emiko Shinagawa, Gunjana Theeragool, Napha Lotong, Kazunobu Matsushita,
... pentitol oxidation by acetic acid bacteria, two different ribitol oxidizing enzymes, one in the cytosolic fraction of ... respect to oxidative fermentation. The cytoplasmic NAD-dependent ribitol dehydrogenase (EC 1.1.1.56) was crystallized ... The enzyme catalyzed a shuttle reversible oxidoreduction between ribitol and D-ribulose in the presence of NAD ... by the enzyme with reaction rates comparable to ribitol oxidation. D-Ribulose, L-ribulose, and L-xylulose ... was found at pH 6.0. NAD-Dependent ribitol dehydrogenase seemed to be specific to oxidoreduction between ...
Tópico(s): Biofuel production and bioconversion
2001 - Oxford University Press | Bioscience Biotechnology and Biochemistry
Michela Zolli, Daniel J. Kobric, Eric D. Brown,
CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of d-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated ... the order of the reactions catalyzed by CDP-ribitol synthase and conducted experiments to resolve the question ... 5-phosphate followed by cytidylyl transfer to d-ribitol 5-phosphate. Steady state kinetic measurements revealed a ... fold kinetic preference for cytidylyl transfer to d-ribitol 5-phosphate over d-ribulose 5-phosphate. Rapid ...
Tópico(s): Polyamine Metabolism and Applications
2001 - American Chemical Society | Biochemistry
Hirokazu Yagi, Chu‐Wei Kuo, Takayuki Obayashi, Satoshi Ninagawa, Kay‐Hooi Khoo, Koichi Kato,
... the two sites, with and without a single ribitol phosphate (RboP) extending from terminal HexNAc. At much ... the two sites, with and without a single ribitol phosphate (RboP) extending from terminal HexNAc. At much ... mannose glycan on a-dystroglycan contains a phospho-ribitol primed for matriglycan addition.Elife. 2016; 5: e14473Crossref ... T. Identification of a post-translational modification with ribitol-phosphate and its defect in muscular dystrophy.Cell ... Schaftingen E. Bommer G.T. ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol ...
Tópico(s): Biotin and Related Studies
2016 - Elsevier BV | Molecular & Cellular Proteomics
Isabelle Gerin, Benoît Ury, Isabelle Breloy, C. Bouchet-Séraphin, Jennifer Bolsée, Mathias Halbout, Julie Graff, Didier Vertommen, Giulio G. Muccioli, Nathalie Seta, Jean‐Marie Cuisset, Ivana Dabaj, Susana Quijano‐Roy, Ammi Grahn, Emile Van Schaftingen, Guido T. Bommer,
... enzymes mutated in dystroglycanopathies can collaborate to attach ribitol phosphate onto α-dystroglycan. Specifically, we demonstrate that isoprenoid synthase domain-containing protein (ISPD) synthesizes CDP-ribitol, present in muscle, and that both recombinant fukutin ( ... and fukutin-related protein (FKRP) can transfer a ribitol phosphate group from CDP-ribitol to α-dystroglycan. We also show that ISPD and FKTN are essential for the incorporation of ribitol into α-dystroglycan in HEK293 cells. Glycosylation of ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2016 - Nature Portfolio | Nature Communications
Anja Follens, Maria Veiga‐da‐Cunha, Rita Merckx, Emile Van Schaftingen, Johan Van Eldere,
... type b Orf1, previously shown to have CDP-ribitol pyrophosphorylase activity (J. Van Eldere, L. Brophy, B. ... Escherichia coli . Cells expressing this protein displayed both ribitol 5-phosphate dehydrogenase and CDP-ribitol pyrophosphorylase activities, whereas these activities were not detectable ... to near homogeneity and found to copurify with ribitol 5-phosphate dehydrogenase and CDP-ribitol pyrophosphorylase activities. These had superimposable elution profiles from ... phosphate and NADPH in one direction and for ribitol 5-phosphate and NADP + in the other direction ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
1999 - American Society for Microbiology | Journal of Bacteriology
Walinka van Tol, Monique van Scherpenzeel, Mohammad Alsady, Moniek Riemersma, Esther Hermans, Else Kragt, Giorgio Tasca, Erik‐Jan Kamsteeg, Maartje Pennings, Ellen van Beusekom, R. Jeroen Vermeulen, Hans van Bokhoven, Nicol C. Voermans, Michèl A.A.P. Willemsen, Angel Ashikov, Dirk J. Lefeber,
Many muscular dystrophies currently remain untreatable. Recently, dietary ribitol has been suggested as a treatment for cytidine diphosphate (CDP)-l-ribitol pyrophosphorylase A (CRPPA, ISPD), fukutin (FKTN), and fukutin-related protein (FKRP) myopathy, by raising CDP-ribitol concentrations. Thus, to facilitate fast diagnosis, treatment development, and treatment monitoring, sensitive detection of CDP-ribitol is required.An LC-MS method was optimized for CDP-ribitol in human and mice cells and tissues.CDP- ...
Tópico(s): Glycogen Storage Diseases and Myoclonus
2019 - American Association for Clinical Chemistry | Clinical Chemistry
Marcela P. Cataldi, Anthony Blaeser, Peijuan Lu, Victoria Leroy, Qi Long Lu,
... the most common causes of dystroglycanopathy. FKRP transfers ribitol-5-phosphate to the O-mannosyl glycan on α-DG from substrate cytidine diphosphate (CDP)-ribitol, which is synthesized by isoprenoid synthase domain-containing ... ISPD). We previously reported that oral administration of ribitol restores therapeutic levels of functional glycosylation of α- ... overexpression of ISPD to the levels of CDP-ribitol and F-α-DG with and without ribitol supplementation in the disease model. ISPD overexpression alone ...
Tópico(s): Exercise and Physiological Responses
2019 - Cell Press | Molecular Therapy — Methods & Clinical Development
Pei Lu, Jason D. Tucker, Elizabeth K. Branch, Fei Guo, Anthony Blaeser, Qi Long Lu,
... cell growth and tumorigenicity. We recently demonstrated that ribitol, considered to be a metabolic end-product, enhances ... the current study, we tested the hypothesis that ribitol could also enhance matriglycan expression in cancer cells. Our results showed for the first time that ribitol is able to significantly enhance the expression of ... on α-DG in breast cancer cells. The ribitol effect is associated with an increase in levels of CDP-ribitol, the substrate for the ribitol-5-phosphate transferases ...
Tópico(s): Ubiquitin and proteasome pathways
2020 - Nature Portfolio | Scientific Reports
Benoît Ury, Sven Potelle, Francesco Caligiore, Matthew R. Whorton, Guido T. Bommer,
... is the only mammalian glycan known to contain ribitol phosphate groups. Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan. Since CDP-ribitol is synthesized in the cytoplasm, we hypothesized that ... into the Golgi apparatus. We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter ... bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol. To test this, we expressed SLC35A1 with mutations ...
Tópico(s): Neuroscience and Neuropharmacology Research
2021 - Elsevier BV | Journal of Biological Chemistry
Hideki Tokuoka, Rieko Imae, Hitomi Nakashima, Hiroshi Manya, Chiaki Masuda, Shunsuke Hoshino, Kazuhiro Kobayashi, Dirk J. Lefeber, Riki Matsumoto, Takashi Okada, Tamao Endo, Motoi Kanagawa, Tatsushi Toda,
Abstract Ribitol-phosphate modification is crucial for the functional maturation of α-dystroglycan. Its dysfunction is associated with muscular ... treatments are currently available for diseases caused by ribitol-phosphate defects. In this study, we demonstrate that ... ISPD ), which encodes an enzyme that synthesizes CDP-ribitol, a donor substrate for ribitol-phosphate modification. We generated skeletal muscle-selective Ispd ... mice, leading to a pathogenic reduction in CDP-ribitol levels, abnormal glycosylation of α-dystroglycan, and severe ...
Tópico(s): Ubiquitin and proteasome pathways
2022 - Nature Portfolio | Nature Communications
S. B. Hulley, Sten Bay Jørgensen, E. C. C. Lin,
Ribitol dehydrogenase from Aerobacter aerogenes strain 1033 acted only on ribitol, but was induced both by ribitol and by d-arabitol. The induction of this ... depend on its metabolism. A mutant which produced ribitol dehydrogenase constitutively was isolated, in which the formation ... although catabolite repression continued to exert its influence. Ribitol dehydrogenase was obligatory for the utilization of ribitol under both aerobic and anaerobic conditions. Under anaerobic conditions the growth rate on ribitol was severely limited by inadequate hydrogen acceptors and ...
Tópico(s): Enzyme Catalysis and Immobilization
1963 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
W T Charnetzky, Robert P. Mortlock,
... inducible pathways for the degradation of the pentitols ribitol and d -arabitol. These pathways are closely linked ... PW52. There are two regulatory sites for the ribitol catabolic pathway as defined by loci for mutations to constitutive synthesis of ribitol dehydrogenase and d -ribulokinase, rbtB and rbtC . The ... and rbtD represent the corresponding genes for the ribitol pathway. The two mutations that lead to constitutive ... can be reduced by increasing the levels of ribitol dehydrogenase either by induction with ribitol or by ...
Tópico(s): Pancreatic function and diabetes
1974 - American Society for Microbiology | Journal of Bacteriology
Kohei Yokoyama, Tomoyuki Miyashita, Yoshio Araki, Eiji Ito,
... of linkage unit intermediates and their functions in ribitol teichoic acid biosynthesis were studied with membranes obtained ... The formation of labeled polymer from CDP-[14C]ribitol and CDP-glycerol in each membrane system was ... GlcNAc-PP-prenol served as an acceptor for ribitol phosphate units, but (glycerol phosphate)-ManNAc-[14C]GlcNAc- ... PP-prenol served as a better acceptor for ribitol phosphate units than (glycerol phosphate)2-ManNAc-[14C]GlcNAc-PP-prenol. In this membrane system (ribitol phosphate)-(glycerol phosphate)-ManNAc-[14C]GlcNAc-PP-prenol ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
1986 - Wiley | European Journal of Biochemistry
Félix Omeñaca, José García-Sicilia, Pilar Garcı́a-Corbeira, Reyes Boceta, V. Garcia Torres,
... booster dose at 18 to 20 months. Antipolyribosyl ribitol phosphate antibody concentrations were determined in serum samples ... primary and booster vaccination.Postprimary seroprotection rates (antipolyribosyl ribitol phosphate > or = 0.15 microg/mL) were lower ... infants (92.5% vs 97.8%), with antipolyribosyl ribitol phosphate geometric mean concentrations of 2.241 vs ... mL was considered. Prebooster seroprotection rates and antipolyribosyl ribitol phosphate geometric mean concentrations were low in both groups (antipolyribosyl ribitol phosphate > or = 1.0 microg/mL in 10. ...
Tópico(s): Influenza Virus Research Studies
2006 - American Academy of Pediatrics | PEDIATRICS
Jason D. Tucker, Ravi Doddapaneni, Pei Lu, Qi Long Lu,
... of mannosylation of α-dystroglycan with the metabolite ribitol in breast cancer lines. Here we investigate the effects of pentose sugars ribitol, ribose, and xylitol media supplementation in breast cancer ... distinctive patterns of alterations in metabolic pathways by ribitol, contrasted with the closely related pentose ribose and pentitol xylitol. Significantly, ribitol supplementation enhances utilization of glucose by glycolysis, whereas ribose improves oxidative phosphorylation and fatty acid synthesis. Ribitol supplementation also increased levels of reduced glutathione (associated ...
Tópico(s): Cancer-related Molecular Pathways
2022 - Public Library of Science | PLoS ONE
Robert P. Mortlock, Dexter D. Fossitt, David H. Petering, W.A. Wood,
... d -Ribulokinase was purified from cells grown on ribitol, d -arabitol, and xylitol, and from a mutant constitutive for ribitol dehydrogenase; d -xylulokinase, from cells grown on d - ... l -xylulokinase, after growth on l -xylose. Similarly, ribitol dehydrogenase was purified after growth on ribitol, d -arabinose, and xylitol, and from the ribitol dehydrogenase-constitutive mutant. d -Arabitol dehydrogenase was obtained ... distinguished only by their substrate and immunological specificity. Ribitol dehydrogenase and d -arabitol dehydrogenase could be distinguished ...
Tópico(s): Enzyme Structure and Function
1965 - American Society for Microbiology | Journal of Bacteriology
W T Charnetzky, Robert P. Mortlock,
... is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (RDH) and d -ribulokinase (DRK). These two ... carbon and energy source, mutants constitutive for the ribitol pathway are able to utilize RDH to oxidize ... d -ribulose by using the DRK of the ribitol pathway. Derivatives of an l -fucose isomerase-constitutive mutant were plated on d -arabinose, ribitol, and xylitol to select and identify mutations in the ribitol pathway. Using the transducing phage PW52, we were ...
Tópico(s): Polyamine Metabolism and Applications
1974 - American Society for Microbiology | Journal of Bacteriology
Mark P. Pereira, Michael A. D’Elia, Justyna Troczynska, Eric D. Brown,
... a putative duplication of genes linked to CDP-ribitol synthesis (tarI'J' and tarIJ) as well as poly(ribitol phosphate) polymerization (tarK and tarL) in S. aureus. ... to create mutants in either of the putative ribitol phosphate polymerases (encoded by tarK and tarL) without ... aureus cell wall. Although genes linked to CDP-ribitol synthesis are also duplicated, a null mutant in ... which catalyzes the rate-limiting step in CDP-ribitol formation. This work provides new insights into understanding the complex synthetic steps of the ribitol phosphate polymer in S. aureus and has implications ...
Tópico(s): Biochemical and Structural Characterization
2008 - American Society for Microbiology | Journal of Bacteriology
Christian Draing, Markus Pfitzenmaier, Sebastiana Zummo, Giuseppe Mancuso, Armin Geyer, Thomas Härtung, Sonja von Aulock,
... by a pentamer repeating unit consisting of one ribitol and a tetrasaccharide carrying the unusual substituents phosphocholine ... of LTA from R6 but showed that its ribitol carries an N-acetyl-d-galactosamine substituent. The ... from Fp23 indicate that this LTA additionally contains ribitol-bound d-alanine. Dose-response curves of the ... by a pentamer repeating unit consisting of one ribitol and a tetrasaccharide carrying the unusual substituents phosphocholine ... of LTA from R6 but showed that its ribitol carries an N-acetyl-d-galactosamine substituent. The ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2006 - Elsevier BV | Journal of Biological Chemistry
Marcela P. Cataldi, Peijuan Lu, Anthony Blaeser, Qi Long Lu,
... with aberrant neuronal development. Here we report that ribitol, a pentose alcohol with previously unknown function in ... with severe congenital muscular dystrophy. Oral administration of ribitol increases levels of ribitol-5-phosphate and CDP-ribitol and restores therapeutic levels of F-α-DG in skeletal and cardiac muscles. Furthermore, ribitol, given before and after the onset of disease ... and respiratory functions in the FKRP mutant mice. Ribitol treatment presents a new class, low risk, and ...
Tópico(s): Sirtuins and Resveratrol in Medicine
2018 - Nature Portfolio | Nature Communications
Donald Trimbur, Robert P. Mortlock,
... of E. coli K-12 which contained a ribitol operon. L-ribose-positive strains of E. coli, ... induce the enzymes of the L-arabinose and ribitol pathways, but only ribitol-negative mutants derived from strains originally L-ribose ... grow on L-ribose, showing that a functional ribitol pathway was required. One of the mutations permitting ... NADPH-linked reductase which converted L-ribose to ribitol. L-ribose is not metabolized by an isomerization ... metabolized by the reduction of L-ribose to ribitol, followed by the conversion to D-ribulose by ...
Tópico(s): Enzyme Structure and Function
1991 - American Society for Microbiology | Journal of Bacteriology
Ranjitha Singh, Raushan Kumar Singh, Sang-Yong Kim, Sujan Sigdel, Jihyun Park, Joon-Ho Choi, In‐Won Kim, Jung-Kul Lee,
... does not meet the increasing demand. In addition, ribitol oxidization for the production of d-ribulose has ... this study, we have employed the Enterobacter aerogenes ribitol: NAD+ 2-oxidoreductase (EaRDH), which could efficiently and selectively convert ribitol to d-ribulose. The purified EaRDH enzyme and ... cell catalyst) were used to produce d-ribulose. Ribitol was efficiently converted to d-ribulose by EaRDH ... and in vitro production of d-ribulose from ribitol. The oxidation of ribitol to d-ribulose by ...
Tópico(s): Amino Acid Enzymes and Metabolism
2016 - Elsevier BV | Biochemical Engineering Journal