Jediael Ng, Zhijun Guo, Oliver Mueller‐Cajar,
The photosynthetic CO2 fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) forms dead-end inhibited complexes while binding multiple sugar phosphates, including its substrate ribulose 1,5-bisphosphate. Rubisco can be rescued from ... the AAA+ hexamer. The photosynthetic CO2 fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) forms dead- ... while binding multiple sugar phosphates, including its substrate ribulose 1,5-bisphosphate. Rubisco can be rescued from ... Rubisco. The enzyme binds the five carbon sugar ribulose 1,5-bisphosphate (RuBP), adds a carbon dioxide ...
Tópico(s): Plant biochemistry and biosynthesis
2020 - Elsevier BV | Journal of Biological Chemistry
Ranjitha Singh, Raushan Kumar Singh, Sang-Yong Kim, Sujan Sigdel, Jihyun Park, Joon-Ho Choi, In‐Won Kim, Jung-Kul Lee,
d-Ribulose, a potentially valuable rare sugar and an excellent building block in asymmetric synthesis, is usually produced by ... addition, ribitol oxidization for the production of d-ribulose has not yet become an industrial reality because ... could efficiently and selectively convert ribitol to d-ribulose. The purified EaRDH enzyme and the recombinant Escherichia ... whole-cell catalyst) were used to produce d-ribulose. Ribitol was efficiently converted to d-ribulose by EaRDH with a yield of ∼85%, suggesting ...
Tópico(s): Amino Acid Enzymes and Metabolism
2016 - Elsevier BV | Biochemical Engineering Journal
Miia Helanto, Kristiina Kiviharju, Matti Leisola, Antti Nyyssölä,
L-Ribulose is a rare and expensive sugar that can be used as a precursor for the production of ... work we describe a production process for L-ribulose using L-arabinose, a common component of polymers ... mutant were used for the production of L-ribulose. The isomerization of L-arabinose to L-ribulose was very unfavorable for L-ribulose formation. However, high L-ribulose yields were obtained by complexing the produced L-ribulose with borate. The process for L-ribulose production in borate buffer by resting cells was ...
Tópico(s): Enzyme Structure and Function
2007 - American Society for Microbiology | Applied and Environmental Microbiology
Spencer M. Whitney, Robert E. Sharwood,
... subunit (L) of the photosynthetic CO2-fixing enzyme ribulose 1,5-bisphospahte carboxylase/oxygenase (Rubisco) is now ... carboxylation rate, and Michaelis constants for CO2 and ribulose 1,5-bisphosphate for the cyanobacterial Rubisco complexes ... subunit (L) of the photosynthetic CO2-fixing enzyme ribulose 1,5-bisphospahte carboxylase/oxygenase (Rubisco) is now ... carboxylation rate, and Michaelis constants for CO2 and ribulose 1,5-bisphosphate for the cyanobacterial Rubisco complexes ... efficiency of the photosynthetic CO2-fixing enzyme d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4. ...
Tópico(s): Algal biology and biofuel production
2006 - Elsevier BV | Journal of Biological Chemistry
Ritva Verho, Mikko Putkonen, John Londesborough, Merja Penttilä, Peter Richard,
... l-xylulose to xylitol. It also converts d-ribulose to d-arabinitol but has no activity with ... The Km values for l-xylulose and d-ribulose are 9.6 and 4.7 mm, respectively. ... l-xylulose to xylitol. It also converts d-ribulose to d-arabinitol but has no activity with ... The Km values for l-xylulose and d-ribulose are 9.6 and 4.7 mm, respectively. ... enzymes l-arabinose isomerase, l-ribulokinase, and l-ribulose-5-phosphate 4-epimerase convert l-arabinose to ... at least in triplicate. The Km for d-ribulose was 4.7 ± 0.8 mm, and the ...
Tópico(s): Polyamine Metabolism and Applications
2004 - Elsevier BV | Journal of Biological Chemistry
... to carbon dioxide, was a new pentose ester, ribulose 5-phosphate, which was then converted to ribose ... in our purified dehydrogenase preparations. The separation of ribulose phosphate from ribose phosphate and the demonstration that ... the sugar in the new pentose ester as ribulose was based on a number of criteria, including ... suggested cleavage of a 5-carbon sugar, probably ribulose 5-phosphate, between carbon atoms 2 and 3. ... using radioactive carbon dioxide, they had identified both ribulose diphosphate and a 7-carbon sugar, sedoheptulose monophosphate, ...
Tópico(s): Enzyme function and inhibition
2002 - Elsevier BV | Journal of Biological Chemistry
Osao Adachi, Yoshikazu Fujii, Yoshitaka Ano, Duangtip Moonmangmee, Hirohide Toyama, Emiko Shinagawa, Gunjana Theeragool, Napha Lotong, Kazunobu Matsushita,
... a shuttle reversible oxidoreduction between ribitol and D-ribulose in the presence of NAD and NADH, respectively. ... with reaction rates comparable to ribitol oxidation. D-Ribulose, L-ribulose, and L-xylulose were well reduced by the ... not oxidized by this enzyme. However, no D-ribulose accumulation was observed outside the cells during the growth of the organism on ribitol. L-Ribulose was accumulated in the culture medium instead, as ... was accounted to catalyze ribitol oxidation to D-ribulose in cytoplasm, taking D-ribulose to the pentose ...
Tópico(s): Biofuel production and bioconversion
2001 - Oxford University Press | Bioscience Biotechnology and Biochemistry
Hajime Sugawara, Hiroki Yamamoto, Naoki Shibata, Tsuyoshi Inoue, Sachiko Okada, Chikahiro Miyake, Akiho Yokota, Yasushi Kai,
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) obtained from a thermophilic red ... 294 which are reported to bind the substrate, ribulose 1,5-bisphosphate, form hydrogen bonds characteristic of ... plant enzymes are almost overlapped to each other. Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4. ... 294 which are reported to bind the substrate, ribulose 1,5-bisphosphate, form hydrogen bonds characteristic of ... plant enzymes are almost overlapped to each other. Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC4.1. ...
Tópico(s): Microbial metabolism and enzyme function
1999 - Elsevier BV | Journal of Biological Chemistry
H. Ishida, Amane Makino, Tadahiko Mae,
The large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) in the illuminated lysates of wheat (Triticum aestivum L.) chloroplasts is broken down by reactive ... The fragmentation was differentially affected by CO2, Mg2+, ribulose 1,5-bisphosphate, or 2-carboxyarabinitol 1,5- ... in the light. The large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) in the ... The fragmentation was differentially affected by CO2, Mg2+, ribulose 1,5-bisphosphate, or 2-carboxyarabinitol 1,5- ...
Tópico(s): Free Radicals and Antioxidants
1999 - Elsevier BV | Journal of Biological Chemistry
Heather J. Kane, Jean‐Marc Wilkin, Archie R. Portis, T. John Andrews,
Oxidation of D-ribulose-1,5-bisphosphate (ribulose-P2) during synthesis and/or storage produces D-glycero-2,3-pentodiulose-1, 5-bisphosphate (pentodiulose-P2), a potent slow, tight-binding inhibitor of spinach (Spinacia oleracea L.) ribulose-P2 carboxylase/oxygenase (Rubisco). Differing degrees of contamination ... time courses to vary between different preparations of ribulose-P2. With some ribulose-P2 preparations, this compound can be the dominant ... It was produced slowly during frozen storage of ribulose-P2, even at low pH, more rapidly in ...
Tópico(s): Antioxidant Activity and Oxidative Stress
1998 - Oxford University Press | PLANT PHYSIOLOGY
Spencer M. Whitney, Denis C. Shaw, David Yellowlees,
... Yellowlees David 1995Evidence that some dinoflagellates contain a ribulose-1,5-bisphosphate carboxylase / oxygenase related to that ... 1995.0040SectionRestricted accessArticleEvidence that some dinoflagellates contain a ribulose-1,5-bisphosphate carboxylase / oxygenase related to that ... 1995https://doi.org/10.1098/rspb.1995.0040AbstractThe ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit ... S (1998) Phytoplankton Activity Through the Measurement of Ribulose Bisphosphate Carboxylase Gene Expression (RuBisCO) Molecular Approaches to ...
Tópico(s): Protist diversity and phylogeny
1995 - Royal Society | Proceedings of the Royal Society B Biological Sciences
Brigitte Gontero, Marie‐Thérèse Giudici‐Orticoni, Jacques Ricard,
Octameric ribulose bisphosphate carboxylase‐oxygenase binds in an independent manner its substrate (ribulose bisphosphate) and a substrate analog (6‐phosphogluconate). The ... behaviour of the active site becomes different if ribulose bisphosphate carboxylase‐oxygenase is inserted in the five‐enzyme complex previously isolated from chloroplasts. Ribulose bisphosphate carboxylase‐oxygenase then becomes more active than ... protein–protein interactions alters the kinetic behaviour of ribulose bisphosphate carboxylase‐oxygenase. This alteration may be expressed ...
Tópico(s): Metal-Catalyzed Oxygenation Mechanisms
1994 - Wiley | European Journal of Biochemistry
Robert L. Houtz, Malcolm Royer, Michael E. Salvucci,
The large subunit (LS) of tobacco (Nicotiana rustica) ribulose-1,5-bisphosphate carboxylase/oxygenase (ribulose-P(2) carboxylase) contains a trimethyllysyl residue at ... 14, whereas this position is unmodified in spinach ribulose-P(2) carboxylase. A protein fraction was isolated ... adenosyl-[methyl-(3)H]-l-methionine to spinach ribulose-P(2) carboxylase. (3)H-Methyl groups incorporated into spinach ribulose-P(2) carboxylase were alkaline stable but could ... N-terminal peptide from the LS of spinach ribulose-P(2) carboxylase contained the site of methylation, ...
Tópico(s): Photosynthetic Processes and Mechanisms
1991 - Oxford University Press | PLANT PHYSIOLOGY
Daryl L. Edmondson, Murray R. Badger, T. John Andrews,
... was initiated by exposure to its substrate, d-ribulose-1,5-bisphosphate (ribulose-P(2)). The decline continued exponentially, with a ... initial rate ratio and the half-time. Varying ribulose-P(2) concentration in the range above that ... of inactivation. The affinities for CO(2) and ribulose-P(2) were unaffected by the inactivation. The decline in activity occurred with preparations of ribulose-P(2) which contained no detectable d-xylulose-1,5-bisphosphate and also with ribulose-P(2) which had been generated enzymatically immediately ...
Tópico(s): Enzyme Structure and Function
1990 - Oxford University Press | PLANT PHYSIOLOGY
Jayashree K. Sainis, Kathleen Merriam, Gary C. Harris,
When Ribulose- 1,5-bisphosphate carboxylase/oxygenase was purified from spinach leaves (Spinacia oleracea) using precipitation with polyethylene glycol ... phosphoribulokinase and 7% of phosphoriboisomerase activities copurified with ribulose- 1,5-bisphosphate carboxylase/oxygenase. This enzyme preparation showed ribose-5-phosphate and ribulose-5-phosphate dependent carboxylase and oxygenase activities which were nearly equivalent to its corresponding ribulose- 1,5-bisphosphate dependent activity. The ribose-5- ...
Tópico(s): Amino Acid Enzymes and Metabolism
1989 - Oxford University Press | PLANT PHYSIOLOGY
B. Lehnherr, Félix Mächler, A. Grandjean, Jürg Fuhrer,
... the determination of the steady state levels of ribulose 1,5-bisphosphate, 3-phosphoglycerate, triose-phosphate, ATP, ADP, AMP, and activity of ribulose, 1,5-bisphosphate carboxylase/oxygenase. Photosynthesis of flag ... associated with a decrease in chlorophyll, soluble protein, ribulose bisphosphate carboxylase/oxygenase activity, ribulose bisphosphate, and adenylates. No decrease was found for ... leaves showed an increased degree of activation of ribulose bisphosphate carboxylase/oxygenase and a decreased ratio of ...
Tópico(s): Air Quality and Health Impacts
1988 - Oxford University Press | PLANT PHYSIOLOGY
Christine H. Foyer, Robert T. Furbank, David A. Walker,
Changes in the concentrations of glycerate 3-phosphate, ribulose 1,5-bisphosphate, fructose 1,6-bisphosphate and ... was then used to study metabolite regulation of ribulose-1,5-bisphosphate carboxylase. The effects of physiological ... fructose 1,6-bisphosphate and dihydroxyacetone phosphate on ribulose-1,5-bisphosphate carboxylase activity of isolated stromal protein at saturating and limiting ribulose 1,5-bisphosphate were measured by incorporation of 14CO2. A system containing ribulose 5-phosphate and an ATP-generating mechanism is ...
Tópico(s): Light effects on plants
1987 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
Robert T. Furbank, Christine H. Foyer, David A. Walker,
The capacity of ribulose-1,5-bisphosphate carboxylase to bind reversibly chloroplast metabolites which are the substrates for both thylakoid and stromal enzymes ... chloroplasts and chloroplast extracts and with pure wheat ribulose-1,5-bisphosphate carboxylase. Measurements of the rate ... effects of binding ADP and inorganic phopshate to ribulose-1,5-bisphosphate carboxylase. The presence of ribulose-1,5-bisphosphate carboxylase significantly altered the values ... while in ‘leaky’ chloroplasts preincubated with KCN and ribulose 1,5-bisphosphate the value was decreased to ...
Tópico(s): Light effects on plants
1987 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
... of the light harvesting chlorophyll alb complex and ribulose biphosphate carboxylase/oxygenase in greening pea leaves. J. ... multi-gene family encoding the small subunit of ribulose-1–5 bisphosphate carboxylase in soybean. J. Molec. ... Chua (1984) Light-regulated expression of a pea ribulose 1–5-bisphosphate carboxylase small subunit gene in ... pea nuclear gene encoding the small subunit of ribulose-1–5 bisphosphate carboxylase. EMBO Journal 3, 1671– ... 1984) Single gene for the large subunit of ribulose bisphosphate carboxylase in maize yields two differentially regulated ...
Tópico(s): Plant nutrient uptake and metabolism
1985 - Wiley | Photochemistry and Photobiology
Cheryl A. Janson, Ward W. Smith, David Eisenberg, F.C. Hartman,
Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39)from Rhodospirillum rubrum has been crystallized ... of the large subunit of a higher plant ribulose-1,5-bisphosphate carboxylase/ oxygenase (477 residues in ... the structure of either the plant or bacterial ribulose-1,5-bisphosphate carboxylasefoxygenase should aid in learning the structure of the other.Ribulose-1,5-bisphosphate carboxylase/oxygenase catalyzes the initial ... and in a variety of photosynthetic microorganisms.The ribulose-1,5-bisphosphate carboxylase/oxygenase from the purple ...
Tópico(s): Photosynthetic Processes and Mechanisms
1984 - Elsevier BV | Journal of Biological Chemistry
... the time before freezing. During induction of photosynthesis, ribulose 1,5-bisphosphate levels increased in parallel with ... In the steady state, a similar relation between ribulose 1,5-bisphosphate levels and CO2 uptake was ... light intensity increased CO2 fixation rates but not ribulose 1,5-bisphosphate levels. Increasing the CO2 concentration resulted in increased CO2 uptake, whereas ribulose 1,5-bisphosphate levels decreased. Even under CO2 saturation, ribulose 1,5-bisphosphate levels were about 100 nmol/ ...
Tópico(s): Marine and coastal ecosystems
1984 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
... effect of subsequent illumination on photosynthetic oxygen evolution, ribulose bisphosphate carboxylase activity, ATP content, and ribulose bisphosphate content was investigated. Inorganic phosphate had little effect on ribulose bisphosphate carboxylase activity in darkness or during the ... phosphate to chloroplasts illuminated without phosphate restored the ribulose bisphosphate carboxylase activity, increased the ATP, and decreased the ribulose bisphosphate in the organelles. The responses to CO2, ...
Tópico(s): Soil Carbon and Nitrogen Dynamics
1984 - Oxford University Press | Journal of Experimental Botany
Douglas B. Jordan, Raymond Chollet,
Substrate ribulose bisphosphate is a potent and a weak inhibitor of the rate of CO2/Mg2+ activation in the ... respectively.At 2 "C, the concen- tration of ribulose bisphosphate required for 50% inhibition of the initial ... rubrum carboxylase.Activator 14C02 trapping experiments demonstrated that ribulose bisphosphate inhibits activation by excluding activator COz from ... enzyme forms which indicated that the K D (ribulose bisphosphate) was 0.02 1 p~ for spinach ... not explained by the equilibrium binding results alone.Ribulose bisphosphate was observed to dissociate very slowly from ...
Tópico(s): Coenzyme Q10 studies and effects
1983 - Elsevier BV | Journal of Biological Chemistry
Andreas Gardemann, Mark Stitt, H. W. Heldt,
... stromal metabolites on the light-activated form of ribulose-5-phosphate kinase was studied with the enzyme ... the enzyme is competitively inhibited with respect to ribulose 5-phosphate by 6-phosphogluconate, ribulose 1,5-bisphosphate, 3-phosphoglycerate and phosphate. Also, ... 6-bisphosphate is inhibitory. All these compounds, except ribulose 1,5-bisphosphate, show an increasing inhibitory effect ... values. Therefore, in the presence of these inhibitors, ribulose-5-phosphate kinase becomes strongly pH dependent. These ...
Tópico(s): Photoreceptor and optogenetics research
1983 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
Richard C. Sicher, A. L. Hatch, David K. Stumpf, Richard G. Jensen,
Ribulose 1,5-bisphosphate in the chloroplast has been suggested to regulate the activity of the ribulose bisphosphate carboxylase/oxygenase. To generate high levels of ribulose bisphosphate, isolated and intact spinach chloroplasts were illuminated ... conditions, chloroplasts generate internally up to 300 nanomoles ribulose 1,5-bisphosphate per milligram chlorophyll if O( ... also absent. This is equivalent to 12 millimolar ribulose bisphosphate, while the enzyme, ribulose bisphosphate carboxylase, offers ...
Tópico(s): Light effects on plants
1981 - Oxford University Press | PLANT PHYSIOLOGY
Richard C. Sicher, Richard G. Jensen,
The response of ribulose 1,5-bisphosphate levels and CO(2) fixation rates in isolated, intact spinach chloroplasts to pyrophosphate, triose phosphates, dl- ... minute in the light, a rapid accumulation of ribulose bisphosphate was measured in most preparations of intact ... CO(2) fixation and also the levels of ribulose bisphosphate. CO(2) fixation was inhibited by dl-glyceraldehyde and O(2) with corresponding decreases in ribulose bisphosphate. When the rate of photosynthesis decreased at limiting irradiances (low light), the level of ribulose bisphosphate in the chloroplast did not always decrease, ...
Tópico(s): Marine and coastal ecosystems
1979 - Oxford University Press | PLANT PHYSIOLOGY
Ribulose-1,5-bisphosphate carboxylase was allowed to react with [2-18O]ribulose 1,5-bisphosphate in the presence of either ( ... dioxide and in the 2-keto position of ribulose 1,5-bisphosphate. This result indicates that the oxygen atom at carbon-3 of ribulose 1,5-bisphosphate is retained during the carboxylation ... phosphoglycerate derived from carbon atoms 3–5 of ribulose 1,5-bisphosphate. In the latter case, significant ( ... indicating that the 2-keto oxygen atom of ribulose 1,5-bisphosphate is retained during the carboxylation ...
Tópico(s): Microbial metabolism and enzyme function
1978 - Wiley | European Journal of Biochemistry
William A. Laing, John T. Christeller,
... dependent interconversions between active and inactive states of ribulose 1,5-bisphosphate carboxylase is presented. It was found that ribulose bisphosphate oxygenase and ribulose bisphosphate carboxylase could be totally inactivated by excluding ... and the rate was inversely related to the ribulose bisphosphare concentration. The initial rate of fully activated ... showed normal Michaelis-Menten kinetics with respect to ribulose bisphosphate (Km = 10muM). Activation was shown to depend ... 10muM. By studying activation and de-activation of ribulose bisphosphate carboxylase as a function of CO2 and ...
Tópico(s): Porphyrin Metabolism and Disorders
1976 - Portland Press | Biochemical Journal
Larry W. Peterson, Ray C. Huffaker,
... darkness and in light. Chlorophyll, total soluble protein, ribulose 1,5-diphosphate carboxylase protein and activity each ... lost was accounted for by a decrease in ribulose 1,5-diphosphate carboxylase protein, suggesting that the ... site of degradation early in senescence.Loss of ribulose 1,5-diphosphate carboxylase protein was negatively correlated ... constants. Cycloheximide essentially prevented the loss of chlorophyll, ribulose 1,5-diphosphate carboxylase protein, and activity and ... chloramphenicol had little effect on the loss of ribulose 1,5-diphosphate carboxylase protein or chlorophyll, or ...
Tópico(s): Plant Gene Expression Analysis
1975 - Oxford University Press | PLANT PHYSIOLOGY
Marcia Wishnick, M. Daniel Lane,
Abstract Spinach leaf ribulose 1,5-diphosphate carboxylase is reversibly inhibited by cyanide at low concentration; 10-5 m and 10-4 m ... 5 m) is uncompetitive (anticompetitive) with respect to ribulose 1,5-diphosphate (ribulose-1,5-di-P) and is of mixed ... evidence suggest that cyanide combines readily with enzyme-ribulose-1,5-di-P complex, but not with enzyme. The inference that enzyme-ribulose-1,5-di-P complex reacts with cyanide ... with the use of the gel filtration technique. Ribulose-1,5-di-P uniformly labeled with 14C ...
Tópico(s): Polyamine Metabolism and Applications
1969 - Elsevier BV | Journal of Biological Chemistry