Richard H. Smith, Justin R. Levy, Robert M. Kotin,
... cell density and scalability achievable with Spodoptera frugiperda Sf9 insect cells grown in serum-free suspension culture. ... 13: 1935-1943Crossref PubMed Scopus (351) Google Scholar Sf9 insect cells are infected with three different recombinant ... and cap gene expression during rAAV production in Sf9 insect cellsTo characterize the temporal occurrence and relative ... a time-course analysis was performed in which Sf9 cells grown in suspension culture were co-infected ... 13: 1935-1943Crossref PubMed Scopus (351) Google Scholar Sf9 cells were sampled at various times postinfection and ...
Tópico(s): CAR-T cell therapy research
2009 - Elsevier BV | Molecular Therapy
Shilpa Jain, Xiaoling Zhang, Preeti J. Khandelwal, Aleister J. Saunders, Brian S. Cummings, Peter Oelkers,
... lysophosphatidylcholine acyltransferase 3 (LPCAT3). Expression of LPCAT3 in Sf9 insect cells conferred robust esterification of lysophosphatidylcholine in ... Electrospray ionization mass spectrometric analysis of phospholipids in Sf9 cells expressing LPCAT3 showed a relative increase in ... lysophosphatidylcholine acyltransferase 3 (LPCAT3). Expression of LPCAT3 in Sf9 insect cells conferred robust esterification of lysophosphatidylcholine in ... Electrospray ionization mass spectrometric analysis of phospholipids in Sf9 cells expressing LPCAT3 showed a relative increase in ...
Tópico(s): Sphingolipid Metabolism and Signaling
2009 - Elsevier BV | Journal of Lipid Research
Yasuo Tsunaka, Junko Toga, Hiroto Yamaguchi, Shin‐ichi Tate, Susumu Hirose, Kosuke Morikawa,
... sites of Drosophila melanogaster FACT (dFACT) expressed in Sf9 cells. These sites are densely concentrated in the ... sites of Drosophila melanogaster FACT (dFACT) expressed in Sf9 cells. These sites are densely concentrated in the ... molecular level. Using the dFACT proteins expressed in Sf9 cells, we investigated how the phosphorylation of dSSRP1 ( ... site-directed mutagenesis method (Stratagene). To produce the Sf9-AB-HMG and Sf9-AID proteins, the protease site insertions within the ... bacmids. The bacmid-cellfection complex was transfected into Sf9 cells. The Sf9 insect cells (ovary-derived cells ...
Tópico(s): DNA Repair Mechanisms
2009 - Elsevier BV | Journal of Biological Chemistry
Hirofumi Sonoda, Junken Aoki, Tatsufumi Hiramatsu, Mayuko Ishida, Koji Bandoh, Yuki Nagai, Ryo Taguchi, Keizo Inoue, Hiroyuki Arai,
... specific PLA1(PS-PLA1). When expressed in insect Sf9 cells, this enzyme was recovered from the Triton ... added phospholipid substrates. However, culture medium obtained from Sf9 cells expressing the enzyme was found to activate ... specific PLA1(PS-PLA1). When expressed in insect Sf9 cells, this enzyme was recovered from the Triton ... added phospholipid substrates. However, culture medium obtained from Sf9 cells expressing the enzyme was found to activate ... continuously produced and released into the medium in Sf9-mPA-PLA1 cells. (ii) The production of LPA ...
Tópico(s): Lipid Membrane Structure and Behavior
2002 - Elsevier BV | Journal of Biological Chemistry
Hidekazu Takahashi, Haruhiko Fujiwara,
... is expressed from a recombinant baculovirus, transposes in Sf9 cells into the chromosomal (TTAGG)n sequences, at ... nuclear polyhedrosis virus (AcNPV) in Spodoptera frugiperda 9 (Sf9) cells. Since S.frugiperda belongs to the same ... al., 2001), the retrotransposition into the host cell (Sf9) chromosomal telomeric repeats is expected to occur. Using ... SART1 retrotransposition, we expressed SART1 from AcNPV in Sf9 cells and monitored, using PCR, whether the silkworm SART1 transposed into the Sf9 chromosomal telomeric repeats (Figure 1A). In this heterologous ...
Tópico(s): Plant Virus Research Studies
2002 - Springer Nature | The EMBO Journal
Jurgen F.M. Vanhauwe, Tarita O. Thomas, Richard D. Minshall, Chinnaswamy Tiruppathì, Anli Li, Annette Gilchrist, Eun-Ja Yoon, Asrar B. Malik, Heidi E. Hamm,
... binding to Go proteins when co-expressed in Sf9 membranes. The potential coupling of thrombin receptors to ... binding to Go proteins when co-expressed in Sf9 membranes. The potential coupling of thrombin receptors to ... Gi and Go proteins when co-expressed in Sf9 cell membranes. In addition, we show that Go1minigenes ... UK). [35S]GTPγS was from PerkinElmer Life Sciences. Sf9 cells were grown at 27 °C and at ... 1271-1274Crossref PubMed Scopus (371) Google Scholar). Harvested Sf9 cells were washed with ice-cold 50 mm ...
Tópico(s): Cell Adhesion Molecules Research
2002 - Elsevier BV | Journal of Biological Chemistry
Fiona Grüninger-Leitch, Daniel C. Schlatter, Erich Küng, Peter Nelböck, Heinz Döbeli,
... cleavage enzyme (BACE) have been cloned, expressed in Sf9 cells, and purified to homogeneity. This aspartic protease ... cleavage enzyme (BACE) have been cloned, expressed in Sf9 cells, and purified to homogeneity. This aspartic protease ... rapid purification of the recombinant proteins. Expression in Sf9 insect cells via recombinant baculovirus resulted in higher ... viral DNA was transformed into the insect cells. Sf9 cells were cultured at 27 °C in TC100 ... of BACE and BACE2, 24-liter fermenters of Sf9 cells were infected with a multiplicity of infection ...
Tópico(s): Phytase and its Applications
2002 - Elsevier BV | Journal of Biological Chemistry
Reto Mu ̈ller, Friedrich Altmann, Dapeng Zhou, Thierry Hennet,
... it as a FLAG-tagged recombinant protein in Sf9 insect cells. Glycosyltransferase assays showed thatbrainiacis capable of ... it as a FLAG-tagged recombinant protein in Sf9 insect cells. Glycosyltransferase assays showed thatbrainiacis capable of ... mm)Mock1-aAnti-FLAG bead bound lysate from Sf9 cells infected with mock baculovirus.brn1-bAnti-FLAG bead bound lysate from Sf9 cells infected with brn baculovirus.pmol/min/mlGlc( ... 91-a Anti-FLAG bead bound lysate from Sf9 cells infected with mock baculovirus.1-b Anti- ...
Tópico(s): Lysosomal Storage Disorders Research
2002 - Elsevier BV | Journal of Biological Chemistry
Andreas Gille, Hui-Yu Liu, Stephen R. Sprang, Roland Seifert,
... the β2-adrenoreceptor (β2AR) couples to Gs, and Sf9 insect cell membranes expressing β2AR-Gαs fusion proteins. ... the β2-adrenoreceptor (β2AR) couples to Gs, and Sf9 insect cell membranes expressing β2AR-Gαs fusion proteins. ... GαsS, β2AR-GαsL, and β2AR-Gαolf, expressed in Sf9 insect cells. Fusion proteins provide close proximity of ... SAL, (±)-alprenolol and CTX were obtained from Sigma. Sf9 cells were cultured and infected with recombinant baculoviruses ... were obtained from BioWhittaker (Walkersville, MD). S49 and Sf9 membranes were prepared according to the protocol described ...
Tópico(s): Peptidase Inhibition and Analysis
2002 - Elsevier BV | Journal of Biological Chemistry
Priya Viswanathan, B. Venkaiah, M. Senthil Kumar, Sheeba Rasheedi, Sudhanshu Vrati, Murali Dharan Bashyam, Seyed E. Hasnain,
... mammalian cells. Cells and Virus—Spodoptera frugiperda cells (Sf9) were grown in TNMFH medium (Invitrogen) containing 10% ... Transient Transfection Assays—Transfection of reporter plasmids into Sf9 insect cells was carried out using Lipofectin as ... Shift Assay—Nuclear protein extracts were prepared from Sf9 cells or the mammalian cells using a modified ... enhancer of transcription from the AcMNPV promoters in Sf9 insect cells. To check if hr1 can enhance ... same CMV promoter-containing plasmids were transfected into Sf9 cells, a 2.6-fold enhancement is observed ( ...
Tópico(s): Virus-based gene therapy research
2003 - Elsevier BV | Journal of Biological Chemistry
Andreas Gille, Roland Seifert,
... forskolin-stimulated adenylyl cyclase (AC) in Gαs-expressing Sf9 insect cell membranes and S49 wild-type lymphoma ... forskolin-stimulated adenylyl cyclase (AC) in Gαs-expressing Sf9 insect cell membranes and S49 wild-type lymphoma ... purity available and obtained from Sigma or Fisher. Sf9 cells were cultured and infected with 1:100 ... 80: 678-696Crossref PubMed Scopus (57) Google Scholar). Sf9 membranes were prepared as described (12Seifert R. Lee ... before membrane preparation. S49 membranes were prepared as Sf9 membranes except that S49 cells were disintegrated by ...
Tópico(s): Phosphodiesterase function and regulation
2003 - Elsevier BV | Journal of Biological Chemistry
Danilo Guerini, Bin Pan, Ernesto Carafoli,
... on its properties. The isoform was overexpressed in Sf9 cells, purified on calmodulin columns, and characterized functionally. ... and PMCA4 isoforms. Pulse and chase experiments on Sf9 cells overexpressing the PMCA pumps showed that PMCA1 ... on its properties. The isoform was overexpressed in Sf9 cells, purified on calmodulin columns, and characterized functionally. ... and PMCA4 isoforms. Pulse and chase experiments on Sf9 cells overexpressing the PMCA pumps showed that PMCA1 ... its calpain sensitivity. The pump was expressed in Sf9 insect cells with the help of the baculovirus ...
Tópico(s): Pancreatic function and diabetes
2003 - Elsevier BV | Journal of Biological Chemistry
Adrienn Bodó, Éva Bakos, Flóra Szeri, András Váradi, Balázs Sarkadi,
... and MRP3, expressed at similar high levels in Sf9 cells. In isolated Sf9 membrane vesicles we measured both human MRP2- and ... J. Biol. Chem. 1998; 273: 32167-32175Google Scholar. Sf9 (Spodoptera frugiperda) cells were cultured and infected with ... 768Google Scholar. Membrane Preparation and Immunoblotting—Virus-infected Sf9 cells were harvested, their membranes were isolated and ... of the proteins of isolated membranes obtained from Sf9 cells and separated by SDS gel electrophoresis. The Sf9 cells were infected with the recombinant baculoviruses inducing ...
Tópico(s): Pregnancy and Medication Impact
2003 - Elsevier BV | Journal of Biological Chemistry
Barry E. Knox, Ernesto Salcedo, Katherine Mathiesz, Jodi Schaefer, Wen-Hai Chou, Linda V. Chadwell, W. Clay Smith, Steven G. Britt, Robert B. Barlow,
... cell culture expression systems: mammalian COS1 cells, insect Sf9 cells, and amphibian Xenopus oocytes. We extracted and ... cis-retinal. The protein expressed in COS1 and Sf9 cells appeared to be misfolded, improperly localized, and ... cell culture expression systems: mammalian COS1 cells, insect Sf9 cells, and amphibian Xenopus oocytes. We extracted and ... cis-retinal. The protein expressed in COS1 and Sf9 cells appeared to be misfolded, improperly localized, and ... for amino acids 330-348 from bovine rhodopsin. Sf9 Expression Vector—Lim Rh1-1D4 was transferred into ...
Tópico(s): Neurobiology and Insect Physiology Research
2003 - Elsevier BV | Journal of Biological Chemistry
Lei Zhang, Anil K. Joshi, Sean C. Smith,
... forms of the mitochondrial proteins were expressed in Sf9 cells and purified, the acyl carrier protein was ... forms of the mitochondrial proteins were expressed in Sf9 cells and purified, the acyl carrier protein was ... Expression of the Human Mitochondrial Malonyltransacylase in Insect Sf9 Cells—The amplified constructs encoding MTmit1, MTmit22, and ... generated, and the encoded protein was expressed in Sf9 insect cells using the Bac-to-Bac baculoviral ... frame with the coding sequence. Subcellular Fractionation of Sf9 Cells Expressing the Full-length and Putative N- ...
Tópico(s): Chemical Synthesis and Analysis
2003 - Elsevier BV | Journal of Biological Chemistry
Markus Koglin, Sönke Behrends,
... with the human ॆ1 subunit in the baculovirus/Sf9 system. Spectral, biochemical, and pharmacological analysis shows that ... with the human ॆ1 subunit in the baculovirus/Sf9 system. Spectral, biochemical, and pharmacological analysis shows that ... shows NO-sensitive enzyme activity after expression in Sf9 or HEK-293 cells in the absence of ... of purity, were obtained from Sigma. Products for Sf9 cell culture were from Invitrogen. Cloning of the ... according to the BAC-TO-BACTM System (Invitrogen). Sf9 cells were cultured in Sf-900 II serum- ...
Tópico(s): Aldose Reductase and Taurine
2003 - Elsevier BV | Journal of Biological Chemistry
Dongning He, Stephen Barnes, Charles N. Falany,
... rat liver λZAP cDNA library and expressed in Sf9 insect cells using a baculoviral vector. rBAT displayed ... rat liver λZAP cDNA library and expressed in Sf9 insect cells using a baculoviral vector. rBAT displayed ... cDNA library and the functional enzyme expressed in Sf9 insect cells. The active enzyme was capable of ... was utilized to generate enzymatically active rBAT in Sf9 insect cells. Primers were designed according to the ... generate recombinant baculovirus particles containing rBAT, 9 × 105 Sf9 cells in mid-log phase were seeded in ...
Tópico(s): Metabolism and Genetic Disorders
2003 - Elsevier BV | Journal of Lipid Research
... containing an intrinsic calmodulin (CaM) purified from an Sf9/baculovirus expression system (Chen, P.-F., and Wu, ... containing an intrinsic calmodulin (CaM) purified from an Sf9/baculovirus expression system (Chen, P.-F., and Wu, ... CaM bound to the protein isolated from an Sf9/baculoviral expression system. This mutant was completely CaM- ... Wild-type and mutant eNOSs expressed in an Sf9/baculovirus system were purified by adding an adequate ... chemicals were products of Bio-Rad. Spodoptera frugiperda (Sf9) cells, baculovirus transfer vector (pVL1392) and BaculoGold viral ...
Tópico(s): Photosynthetic Processes and Mechanisms
2003 - Elsevier BV | Journal of Biological Chemistry
Christin E. Bland, Priscilla J. Kimberly, Matthew D. Rand,
... g/liter) (1× BPYE) supplement (Difco).Spodoptera frugiperda (SF9) cells were cultured in Graces medium (JRH Biosciences) ... Dl-S2 and transiently transfected S2, Kc167, and SF9 cells with pIZDlV5 were analyzed. After transient transfection ... lines: DrosophilaS2 and Kc167 lines and the Lepidopteran SF9 cell line. Treatment of DlV5-expressing S2 cells ... 1C). Delta processing also occurs in Kc167 and SF9 cells (Fig.1B). In contrast to the S2 ... the major product in the untreated Kc167 and SF9 cells. Furthermore, a significantly greater amount of the ...
Tópico(s): Congenital Ear and Nasal Anomalies
2003 - Elsevier BV | Journal of Biological Chemistry
Dominique T. Brandt, Axel Goerke, Marion Heuer, Mario Gimona, Michael Leitges, Elisabeth Kremmer, Reiner Lammers, Hermann Haller, Harald Mischak,
... PTP, protein tyrosine phosphatase; GST, glutathione S-transferase; Sf9, Spodoptera frugiperda; MBP, myelin basic protein; pNPP, p- ... PTP, protein tyrosine phosphatase; GST, glutathione S-transferase; Sf9, Spodoptera frugiperda; MBP, myelin basic protein; pNPP, p- ... To express GST-tagged PKCs in Spodoptera frugiperda (Sf9) insect cells, the complete coding regions of the ... with Baculo-Gold (BD PharMingen, Dianova) DNA in Sf9 cells according to the manufacturers' recommendations. Five plaques of each baculovirus were picked and amplified in Sf9 cells. Protein expression in infected cells was examined ...
Tópico(s): Cardiomyopathy and Myosin Studies
2003 - Elsevier BV | Journal of Biological Chemistry
Lucien Gazi, Juan F. López‐Giménez, Martin Rüdiger, Philip G. Strange,
... receptors were then expressed in Spodoptera frugiperda 9 (Sf9) cells using the baculovirus system, and their oligomerization ... Spiperone labelled D2 receptors in membranes prepared from Sf9 cells expressing epitope-tagged D2L or D2S receptors, ... homo-oligomerization of D2L and D2S receptors in Sf9 cells. When the FLAG-tagged D2S and HIV- ... two isoforms can also form hetero-oligomers in Sf9 cells. Time-resolved FRET with europium and XL665-labelled antibodies was applied to whole Sf9 cells and to membranes from Sf9 cells expressing ...
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2003 - Wiley | European Journal of Biochemistry
Christoph Geisler, Jared J. Aumiller, Donald L. Jarvis,
... cloning of genes encoding β-N-acetylglucosaminidases from Sf9 cells, which are a clonal derivative of the ... isolation of three β-N-acetylglucosaminidase genes from Sf9 cells, which were designated SfGlcNAcase-1,-2,-3 ( ... also molecularly cloned two allelic variants of an Sf9 cell β-N-acetylglucosaminidase gene, which they termed ... gene encodes the processing β-N-acetylglucosaminidase of Sf9 cells. In the present report we provide data ... cloned a new β-N-acetylglucosaminidase cDNA from Sf9 cells, which turned out to be the S. ...
Tópico(s): Transgenic Plants and Applications
2008 - Elsevier BV | Journal of Biological Chemistry
... for radioresistance of a lepidopteran insect cell line, Sf9, established from the ovarian tissue of Fall armyworm, ... reactive oxygen species (ROS) contribute to radioresistance of Sf9 cells, besides indicating a radioprotective role of cellular ... the LD50 of 49.7 Gy derived for Sf9 cells is about three times the LD50 value ... to 50 Gy induce transient growth inhibition in Sf9 cells, which gets released during 48—96 h post-irradiation (3). The authors further show that Sf9 cells do not undergo phosphatidylserine externalization (annexin-V ...
Tópico(s): Insect behavior and control techniques
2010 - Oxford University Press | Mutagenesis
Valentina Godovikova, Helena H. Ritchie,
... Pharmingen, San Diego, CA)Google Scholar) into insect Sf9 cells to obtain virus stock. To produce recombinant proteins, insect Sf9 cells infected with recombinant virus stock at a ... purification, the supernatant from pVL941-DSP370 cDNA-infected Sf9 cells was diluted 1:20 with 100% trichloroacetic ... be secreted into the insect culture medium. Insect Sf9 cells were infected with baculovirus containing either DSP- ... from DSP. Dynamic Processing of DSP-PP240 Protein—Sf9 cells were infected for 4 days with baculovirus ...
Tópico(s): Oral and Maxillofacial Pathology
2007 - Elsevier BV | Journal of Biological Chemistry
Noboru Tomiya, Someet Narang, Jung Park, Badarulhisam Abdul‐Rahman, One Choi, Sundeep Singh, Jun Hiratake, Kanzo Sakata, Michael J. Betenbaugh, Karen Palter, Yuan C. Lee,
... acetylhexosaminidase from the culture medium of Spodoptera frugiperda Sf9 cells (Sfhex). The purified Sfhex protein showed 10 ... lysosomal β-N-acetylhexosaminidases. Recombinant Sfhex expressed in Sf9 cells exhibited the same substrate specificity and pH ... Sfhex was secreted into the culture medium by Sf9 cells, a significant amount of Sfhex was also ... β-N-acetylhexosaminidase acting on N-glycans from Sf9 cells. Paucimannosidic glycans are often predominant in N- ... acetylhexosaminidase from the culture medium of Spodoptera frugiperda Sf9 cells (Sfhex). The purified Sfhex protein showed 10 ...
Tópico(s): Trypanosoma species research and implications
2006 - Elsevier BV | Journal of Biological Chemistry
Kei Takahashi, Yasuhisa Kimura, Noriyuki Kioka, Michinori Matsuo, Kazumitsu Ueda,
... HDL formation, we expressed human ABCA1 in insect Sf9 cells and purified it. Trypsin limited-digestion of ... HDL formation, we expressed human ABCA1 in insect Sf9 cells and purified it. Trypsin limited-digestion of ... DPPE, 1,2-dipalmitoylphosphatidylethanolamine; DPPG, 1,2-dipalmitoylphosphatidylglycerol; Sf9, Spodoptera frugiperda 9; HEK, human embryo kidney; NGF, ... by the co-transfection of Spodoptera frugiperda 9 (Sf9) cells with pVL1392-ABCA1-TATH and Baculogold™ DNA. ... in the dark. Expression of Human ABCA1 in Sf9 Cells—Sf9 cells were grown at 27 °C ...
Tópico(s): Systemic Lupus Erythematosus Research
2006 - Elsevier BV | Journal of Biological Chemistry
Kyungmi Yang, Jeong Hak Kim, Hae Jong Kim, Insung Park, Ick Young Kim, Beom-Seok Yang,
... expression vector. Cell Lines and Culture—Spodoptera frugiperda SF9 insect cells (Clontech) were maintained in TNM-FH ... baculoviral expression vector plasmid DNA was transfected into sf9 cells along with viral genomic DNA from a ... a titer of ∼108 plaque-forming units/ml. sf9 cells were infected with multiplicity of infection 10 ... Biosciences) by fast-protein liquid chromatography system. Infected sf9 cells from 400-ml cultures were suspended in ... GST-tagged proteins bound to glutathione beads, infected sf9 cells were lysed in 500 μl of the ...
Tópico(s): Cellular Mechanics and Interactions
2005 - Elsevier BV | Journal of Biological Chemistry
Katrin Schnizler, Beate Saeger, Carsten K. Pfeffer, Alexander Gerbaulet, Ulrich Ebbinghaus‐Kintscher, Christoph Methfessel, Eva-Maria Franken, K. Raming, Christian H. Wetzel, Arunesh Saras, H. Pusch, Hanns Hatt, Günter Gisselmann,
... of pHCl in oocytes of Xenopus laevis and Sf9 cells induces a chloride current with a linear ... of pHCl in oocytes of Xenopus laevis and Sf9 cells induces a chloride current with a linear ... of these splice variants in Xenopus oocytes and Sf9 cells revealed a unique combination of pharmacological and ... the extracellular solution. Cell Culture and Transfection of Sf9 Cells for Patch-clamping Experiments—Sf9 cells were grown at 26 °C in Sf- ... after transfection. Whole Cell Voltage Clamp Experiments on Sf9 Cells—Membrane currents of EGFP-expressing Sf9 cells ...
Tópico(s): Nicotinic Acetylcholine Receptors Study
2005 - Elsevier BV | Journal of Biological Chemistry
Marianne G.S. de Carvalho, Ashley L. McCormack, Eric Olson, Farideh Ghomashchi, Michael H. Gelb, John R. Yates, Christina C. Leslie,
... identified using recombinant cPLA2 expressed in Spodoptera frugiperda (Sf9) cells. Analysis by high performance liquid chromatography of ... 727 using mass spectrometry and automated Edman sequencing. Sf9 cells infected with recombinant virus expressing cPLA2 exhibited ... inhibitor okadaic acid, which was not observed in Sf9 cells infected with wild-type virus. Stimulation of Sf9 cells with A23187 and okadaic acid also increased ... in insect cells. These results show that the Sf9-baculovirus expression system is useful for investigation of ...
Tópico(s): Enzyme Structure and Function
1996 - Elsevier BV | Journal of Biological Chemistry
Ran Zhang, Ellen Buczko, Maria Dufau,
... in both the COS1 cell and the insect Sf9 cell with the soluble splice variant Form B ... Marcel Dekker, NY1976: 175-183Google Scholar). Spodoptera frugipera (Sf9) cells (PharMingen) were co-transfected with 4 μg ... recombinant virus titers were amplified by reinfection of Sf9 cells, and titers were monitored by plaque assay. ... WT and mutant LHRs was achieved by infecting Sf9 cells (7 × 106 cells/75-cm2 flask) with ... Detergent-solubilized Extracts of Transfected COS1 Cells and Sf9 CellsCOS1 Cells48 h after transfection with WT or ...
Tópico(s): Neurobiology and Insect Physiology Research
1996 - Elsevier BV | Journal of Biological Chemistry