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... Items Theatre Prisoners of the pestilence One Flea Sparc Bush Multiple Classified Advertising Items Theatre 2 Donald ...
1995 - Gale Group | TDA
Robert B. Vernon, Helene Sage,
... The 43,000-MW, secreted, calcium-binding glycoprotein SPARC (Secreted Protein, Acidic and Rich in Cysteine) is ... reorganization. Here, we report on the expression of SPARC in the testes of adult mice. By immunohistochemistry, SPARC was observed in the cytoplasm of Leydig cells ... of approximately 42,000 MW that bound anti-SPARC antibodies. Northern blot analysis revealed 2.3 kilobase (kb) SPARC mRNA in the testis, a size comparable to that of SPARC mRNA in nongonadal cells. Western blot assays of ... mouse Leydig cells cultured 24–48 h expressed SPARC in cytoplasmic granules. Cultured Leydig cells incorporated [35S] ...
Tópico(s): Infant Nutrition and Health
1989 - Oxford University Press | Biology of Reproduction
... DOAJ. O financiamento foi fornecido pelo OSI, JISC, SPARC Europe e CURL.##O OpenDOAR é um diretório ...
Lund University. Libraries + University of Nottingham. Information Services
Muhammad Yunus, Aidan Foster-Carter, Erin Mooney, Philip Pascall, Philip Raworth, Robin Hardy, David Green, Linda Bell,
... Handed Airlines in China Face value Losing his Sparc Trial and error Churning heads Chief executives Annual ...
2002 - Gale Group | The Economist
Helene Sage, Robert B. Vernon, Sarah E. Funk, Elizabeth A. Everitt, John C. Angello,
SPARC (Secreted Protein Acidic and Rich in Cysteine) is a Ca+2-binding glycoprotein that is differentially ... migration, and proliferation. We show here that exogenous SPARC, added to cells in culture, was associated with ... aortic endothelial cells, exposed to 2-40 micrograms SPARC/ml per 2 x 10(6) cells, exhibited ... grew in aggregates after replating in media without SPARC. SPARC caused rounding of bovine endothelial cells, fibroblasts, and ... 3T3 were not affected. The activity of native SPARC was inhibited by heat denaturation and prior incubation ...
Tópico(s): Bone health and treatments
1989 - Rockefeller University Press | The Journal of Cell Biology
... Sciences (AIBS), o Scholarly Publishing & Academic Resources Coalition (SPARC), a Universidade do Kansas, a Greater Western Library ...
BioOne
Hironori Uehara, Ling Luo, Jacquelyn M. Simonis, Nirbhai Singh, Ethan Will Taylor, Balamurali K. Ambati,
It is known that SPARC gates VEGF-A signal transduction towards KDR, the primary angiogenic VEGF receptor. We sought to determine whether inhibition of SPARC activity using anti-SPARC peptide could inhibit laser-induced CNV by promoting ... VEGF-A to FLT-1. We created anti-SPARC l-peptide and retro-inverso anti-SPARC d-peptide. Anti-SPARC peptides or PBS were injected intravitreally 1 day ... or after laser induction. Intravitreal injection of anti-SPARC l-peptide 1 day before laser induction promotes ... phosphorylation and inhibited laser-induced CNV and anti-SPARC d-peptide had no effect. Injection 1 day ...
Tópico(s): Angiogenesis and VEGF in Cancer
2009 - Elsevier BV | Vision Research
Amy D. Bradshaw, Catalin F. Baicu, Tyler J. Rentz, An O. Van Laer, Janet M. Boggs, J. Matthew Lacy, Michael R. Zile,
... that secreted protein acidic and rich in cysteine (SPARC) plays a key role in post–synthetic procollagen ... diastolic function are affected by the absence of SPARC, age-matched wild-type (WT) and SPARC-null mice underwent either transverse aortic constriction (TAC) ... morphological structure was examined by scanning electron microscopy. SPARC expression was measured by immunoblot. LV, myocardial, and ... studies. In WT mice, TAC increased LV mass, SPARC expression, myocardial diastolic stiffness, fibrillar collagen content, and ...
Tópico(s): Connective tissue disorders research
2009 - Lippincott Williams & Wilkins | Circulation
Shanna A. Arnold, Rolf A. Brekken,
... many clinical studies have found a correlation of SPARC expression with malignant progression and patient survival, the mechanisms for SPARC function in tumorigenesis and metastasis remain elusive. The activity of SPARC is context- and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression ... studies and in animal models. The capacity of SPARC to dictate tumorigenic phenotype has been attributed to ... remodeling, angiogenesis, immune modulation and metastasis. Given that SPARC is credited with such varied activities, this review ...
Tópico(s): Oral and Maxillofacial Pathology
2009 - Springer Science+Business Media | Journal of Cell Communication and Signaling
Neil Desai, Vuong Trieu, B Damascelli, Patrick Soon‐Shiong,
SPARC up-regulation is a poor prognostic factor in head and neck cancer. It was hypothesized that because of a SPARC-albumin interaction, tumoral SPARC facilitates the accumulation of albumin in the tumor ... by correlating the response to nab-paclitaxel and SPARC tumor expression in a retrospective analysis of a ... the 16 nab-paclitaxel-treated patients (12/16 SPARC-positive, 75%). Response to nab-paclitaxel was higher for SPARC-positive patients (10/12, 83%) than SPARC-negative patients (1/4, 25%). The SPARC-negative ...
Tópico(s): Oral and Maxillofacial Pathology
2009 - Elsevier BV | Translational Oncology
... happen?The Scholarly Publishing and Academic Resources Coalition (SPARC) was formed with this purpose in mind. SPARC, an international alliance of over 800 academic and ... DO or any other journal, consider using the SPARC Author Addendum, available at www.arl.org/sparc/author. This is a legal instrument that modifies ... is also a good source of information on SPARC's education, advocacy, and publisher partner programs in ... happen? The Scholarly Publishing and Academic Resources Coalition (SPARC) was formed with this purpose in mind. SPARC, ...
Tópico(s): Academic Publishing and Open Access
2009 - Elsevier BV | American Journal of Orthodontics and Dentofacial Orthopedics
Seno,
The secreted protein acidic and rich in cysteine (SPARC) is a secreted glycoprotein that plays an essential ... the present study, we investigated the role of SPARC in the motile and invasive activities of human glioma cells by silencing the SPARC gene. Introduction of SPARC-targeted small interfering RNA (siRNA) into glioma cell lines resulted in downregulation of SPARC expression, and significantly suppressed glioma cell migration in ... was significantly reduced in the cells transfected with SPARC siRNA compared with those transfected with control siRNA. ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2009 - Spandidos Publishing | International Journal of Oncology
Daniel D. Von Hoff, Ramesh K. Ramanathan, Mitesh J. Borad, Dan Laheru, Lee Smith, Timothy R. Wood, Ronald L. Korn, Neil Desai, José Iglesias, Manuel Hidalgo,
... cells and surrounding stroma are known to overexpress SPARC (secreted protein acid rich in cysteine), which is ... accumulation of paclitaxel through binding of albumin to SPARC. This disease specific phase 1 study was designed ... P and the correlation of response with tumor SPARC and serum CA19–9 levels. Methods: nab-P ... prior chemotherapy for their metastatic disease. Level 3 SPARC staining by immunohistochemistry was considered positive. Results: 63 ... The median survival was 9 months to date. SPARC data were available for 35 pts, of which ...
Tópico(s): Bone health and treatments
2009 - Lippincott Williams & Wilkins | Journal of Clinical Oncology
G. Chen, Xiaoqing Tian, Z. Liu, Sijie Zhou, B Schmidt, Doris Henne‐Bruns, Max G. Bachem, Marko Kornmann,
Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell–cell and cell–matrix interactions. In cancer, SPARC can be not only associated with a highly ... this study was to characterise the function of SPARC and its modulation by fibroblast growth factor receptor ( ... 1 isoforms in pancreatic ductal adenocarcinoma (PDAC). Exogenous SPARC inhibited growth, movement, and migration. ShRNA inhibition of endogenous SPARC in ASPC-1 and PANC-1 cells resulted ... fibroblast growth factor (FGF) 1 and FGF2. Endogenous SPARC expression in PANC-1 cells was increased in ...
Tópico(s): Pancreatic and Hepatic Oncology Research
2009 - Springer Nature | British Journal of Cancer
Izabela Podgorski, Bruce E. Linebaugh, Jennifer E. Koblinski, Deborah Rudy, Mackenzie K. Herroon, Mary B. Olive, Bonnie F. Sloane,
... K is capable of processing and thereby modulating SPARC, a protein implicated in bone metastasis and inflammation. The coincident up-regulation of SPARC and cathepsin K occurred both in vivo in ... stromal cell interaction increased secretion and processing of SPARC, as did co-cultures of bone marrow stromal ... inhibitors had significantly reduced secretion and cleavage of SPARC. Increases in secretion of pro-inflammatory cytokines (ie, ... cathepsin K in regulation of biological activity of SPARC in bone metastasis. Bone metastasis is a hallmark ...
Tópico(s): Bone Metabolism and Diseases
2009 - Elsevier BV | American Journal Of Pathology
Katarina Kos, Steve Wong, Bee K. Tan, Anders Gummesson, Margareta Jernås, Niclas Franck, David Kerrigan, Fredrik H. Nyström, Lena Carlsson, Harpal S. Randeva, Jonathan Pinkney, John Wilding,
Matricellular Secreted Protein, Acidic and Rich in Cysteine (SPARC), originally discovered in bone as osteonectin, is a ... metabolic dysregulation. Therefore, we tested the hypothesis that SPARC in human adipose tissue is influenced by glucose ... lean control subjects for analysis of metabolic markers, SPARC, and various cytokines (RT-PCR). Additionally, 24 obese ... cultured with recombinant leptin, insulin, and glucose, and SPARC mRNA and protein expression determined by Western blot analyses.SPARC expression in human adipose tissue correlated with fat ...
Tópico(s): Bone Metabolism and Diseases
2009 - American Diabetes Association | Diabetes
Shanna A. Arnold, Lee B. Rivera, Andrew F. Miller, Juliet G. Carbon, Seán Dineen, Yang Xie, Diego H. Castrillón, E. Helene Sage, Pauli Puolakkainen, Amy D. Bradshaw, Rolf A. Brekken,
Utilizing subcutaneous tumor models, we previously validated SPARC (secreted protein acidic and rich in cysteine) as a key component of the stromal response, where it regulated tumor size, angiogenesis and extracellular ... we demonstrate that pancreatic tumors grown orthotopically in Sparc-null (Sparc(-/-)) mice are more metastatic than tumors grown in wild-type (Sparc(+/+)) littermates. Tumors grown in Sparc(-/-) mice display reduced deposition of fibrillar collagens I ... in tumors grown in the absence of host SPARC. However, tumors from Sparc(-/-) mice display increased permeability ...
Tópico(s): Cancer Research and Treatments
2009 - The Company of Biologists | Disease Models & Mechanisms
Evangelos Terpos, Dimitrios Christoulas, Eirini Katodritou, Cornelia Bratengeier, Brigitte Lindner, Silvia Harmelin, Gerhard Hawa, Georgios Boutsikas, Magdalini Migkou, Maria Gavriatopoulou, Eurydiki Michalis, Anastasia Pouli, Efstathios Kastritis, Konstantinos Zervas, Meletios Α. Dimopoulos,
... induced osteoblast differentiation and ectopic bone formation. Osteonectin (SPARC) is a multi-faceted protein that belongs to ... formation, initiating mineralization and promoting mineral crystal formation. SPARC shows affinity for collagen in addition to bone ... possible correlations with clinical and laboratory data, including SPARC levels, ISS stage and survival. One hundred and ... therapy, including bisphosphonates, were evaluated. Serum sclerostin and SPARC were measured using an ELISA methodology developed by ...
Tópico(s): Bone Metabolism and Diseases
2009 - Elsevier BV | Blood
Matthew J. Socha, Neveen Said, Yan‐Shan Dai, Joseph Kwong, Preetha Ramalingam, Vuong Trieu, Neil Desai, Samuel C. Mok, Kouros Motamed,
... carcinomas. Secreted protein acidic and rich in cysteine (SPARC) is reportedly detrimental to the growth of ovarian ... epigenetically silenced in several cancers. We hypothesized that SPARC is downregulated in ovarian cancer through aberrant promoter hypermethylation. To that end, we analyzed SPARC expression in ovarian cancer cell lines and investigated the methylation status of the Sparc promoter using methylation-specific polymerase chain reaction. Our results show that SPARC mRNA expression is decreased in three (33%) and ...
Tópico(s): Cancer-related gene regulation
2009 - Elsevier BV | Neoplasia
Zhong‐Sheng Zhao, Yuanyu Wang, Yongquan Chu, Zai‐yuan Ye, Hou‐Quan Tao,
... of secreted protein, acidic and rich in cysteine (SPARC), in the development and progression of gastric cancer.Immunohistochemistry was used to analyze SPARC, integrin beta1, and matrix metalloproteinase (MMP)-2 expression in 436 clinicopathologically characterized gastric cancer cases.SPARC, integrin beta1, and MMP-2 protein levels were ... gastric cancer lesions compared with adjacent noncancerous tissues. SPARC protein was detected in 334 of 436 human ... also found a positive correlation between expression of SPARC and MMP2, and SPARC and integrin beta1. In ...
Tópico(s): Cancer Research and Treatments
2009 - American Association for Cancer Research | Clinical Cancer Research
Ramez I. Haddadin, Dong‐Jin Oh, Min Hyung Kang, Theodoros Filippopoulos, Meenakashi Gupta, Lois Hart, E. Helene Sage, Douglas J. Rhee,
SPARC is a matricellular protein that is highly expressed in remodeling tissues, including the trabecular meshwork and ... body. The hypothesis for the study was that SPARC contributes to the regulation of intraocular pressure (IOP). The IOPs of SPARC-null mice, their corresponding wild-type (WT), and ... Diurnal and nocturnal IOPs of C57Bl/6x129SvJ WT, SPARC-null, and heterozygous mice were measured. Fluorophotometric measurements ... LM).During the day, the mean IOP of SPARC-null mice (n = 142, 16.9 +/- 2.4 ... 5 mm Hg; P < 10(-4)). At night, SPARC-null mice also exhibited a blunted increase in ...
Tópico(s): Retinal Diseases and Treatments
2009 - Cadmus Press | Investigative Ophthalmology & Visual Science
Kazutaka Horie, Masami Tsuchihara, Tetsuya Nakatsura,
... of secreted protein acidic and rich in cysteine (SPARC) is associated with increased aggressiveness and poor prognosis ... not fully clarified. To validate the potential of SPARC as a therapeutic target, we examined the effect of the knockdown of SPARC with SPARC‐specific siRNA on the growth of human melanoma cell lines. SPARC siRNAs exerted a potent knockdown effect. Silencing of SPARC resulted in growth inhibition with G 1 arrest ... but not in CRL1579 cells. Conditioned media containing SPARC from MeWo cells could not restore the growth ...
Tópico(s): Cancer-related Molecular Pathways
2009 - Wiley | Cancer Science
Süheyla Bozkurt, Erdoğan Ayan, Fatihhan Bolukbasi, İlhan Elmacı, M. Necmettin Pamir, Aydın Sav,
... tumor cell invasion and in progression to malignancy. SPARC (secreted protein, acidic and rich in cysteine) (osteonectin) ... this study was to evaluate the expression of SPARC with proliferation index, p53 reactivity in WHO grade ... Using immunohistochemical analysis, we evaluated the expression of SPARC, Ki-67 (MIB-1) and p53 in meningiomas. Immunohistochemical scores of SPARC were determined as the sum of frequency (0- ... cells. A high immunohistochemical score (4-6) for SPARC was more frequent in atypical and in anaplastic ...
Tópico(s): Bone health and treatments
2009 - Wiley | Apmis
Catalina Atorrasagasti, Mariana Malvicini, Jorge B. Aquino, Laura Alaniz, Mariana Garcı́a, Marcela F. Bolontrade, Manglio Rizzo, Osvaldo L. Podhajcer, Guillermo Mazzolini,
... related death worldwide. Current treatments are extremely disappointing. SPARC (Secreted protein, acidic and rich in cysteine) is ... HepG2, Hep3B and Huh7) with an adenovirus expressing SPARC (AdsSPARC) to examine the role of SPARC expression on HCC cells and its effect on ... proliferation and cell cycle profile were unaffected; however, SPARC overexpression reduced HCC proliferation when cells were grown ... mild induction of cellular apoptosis was observed upon SPARC overexpression. SPARC overexpression resulted in spheroid growth inhibition ...
Tópico(s): Pancreatic and Hepatic Oncology Research
2009 - Wiley | International Journal of Cancer
P Hasselaar, David J. Loskutoff, Michael Sawdey, E. Helene Sage,
SPARC, a Ca(2+)-binding glycoprotein that is expressed during tissue morphogenesis and functions as an inhibitor ... on Western blots with anti-PAI-1 antiserum. SPARC stimulated the secretion of PAI-1 protein into ... values within 24 h after the addition of SPARC. Levels of PAI-1 mRNA were elevated 2- ... 4 to 24 h after the addition of SPARC and did not increase with higher concentrations of SPARC. Since the induction of PAI-1 mRNA by SPARC was not blocked by cycloheximide, de novo protein ... 1 was not due to contamination of the SPARC preparations with endotoxin. These data demonstrate that SPARC ...
Tópico(s): Oral microbiology and periodontitis research
1991 - Elsevier BV | Journal of Biological Chemistry
Maurice Ringuette, Sashko Damjanovski, Donna L. Wheeler,
To explore the biological functions of SPARC (secreted protein, acidic, rich in cysteine), a Ca 2+ -binding extracellular glycoprotein, we have examined its expression in an evolutionary diverse group of organisms. Similar patterns of SPARC mRNA expression were observed in adult mouse and rat tissues. SPARC transcripts represented 0.0002–0.0025% of the ... brain, and heart, whereas relatively low levels of SPARC RNA were detected in liver and kidney. Within ... correlation was observed between the tissue distribution of SPARC and cytoskeletal actin transcripts. Southern blot analysis revealed ...
Tópico(s): Fluoride Effects and Removal
1991 - NRC Research Press | Biochemistry and Cell Biology
Sarah E. Funk, E. Helene Sage,
SPARC (secreted protein, acidic and rich in cysteine) is an extracellular, Ca2(+)-binding protein associated with cellular ... migration, proliferation, and/or differentiation. Active preparations of SPARC bind to specific components of the extracellular matrix ... rounded phenotype. In this study we show that SPARC modulates the progression of bovine aortic endothelial cells ... cycle. At a concentration of 20 micrograms/ml, SPARC inhibited the incorporation of [3H]thymidine into newly ... greater than 90% inhibition at 30 micrograms of SPARC per ml after 24 hr. A 20-residue ...
Tópico(s): Cancer Research and Treatments
1991 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Mario P. Colombo, Giuliana Ferrari, G Biondi, Daniela Galasso, Chin C. Howe, Giorgio Parmiani,
SPARC (secreted protein acid and rich in cysteine), also known as osteonectin or BM-40, is a ... and slot-blot analyses were used to study SPARC expression in tumours induced in vivo by methylcholanthrene ( ... and SV-40 infection. MCA-induced tumours expressed SPARC mRNA at quantitatively different levels. Fibroblasts transformed in ... extent, by SV-40, showed reduced levels of SPARC mRNA expression compared with normal fibroblasts. Run-on assay indicated that transcription of SPARC was lower in the Kirsten-MSV transformed cells ...
Tópico(s): Bone health and treatments
1991 - Pergamon Press | European Journal of Cancer and Clinical Oncology
Timothy F. Lane, M. Luisa Iruela‐Arispe, E. Helene Sage,
... of several extracellular matrix proteins. The expression of SPARC, a secreted collagen-binding glycoprotein, has been shown ... process. We now show that addition of purified SPARC protein, or an N-terminal synthetic peptide (SPARC4- ... the synthesis of type 1-plasminogen activator inhibitor. SPARC decreased fibronectin mRNA by 75% over 48 h, an effect that was inhibited by anti-SPARC immunoglobulins. Levels of thrombospondin-1 mRNA were diminished ... plasminogen activator inhibitor (PAI-1) were enhanced by SPARC and the SPARC4-23 peptide. The effects were ...
Tópico(s): Cell Adhesion Molecules Research
1992 - Elsevier BV | Journal of Biological Chemistry