Naofumi Kajitani, Masaaki Iwata, Akihiko Miura, Kyohei Tsunetomi, Takehiko Yamanashi, Ryoichi Matsuo, Tsuyoshi Nishiguchi, Saki Fukuda, Mayu Nagata, Midori Shibushita, Takahira Yamauchi, Shenghong Pu, Yukihiko Shirayama, Ken Watanabe, Koichi Kaneko,
Abstract Aims Neuroinflammation is deeply related to the pathophysiology of depression. Beta‐hydroxybutyrate (BHB), which is an endogenous ketone body, exerts anti‐inflammatory effects, and peripheral administration of BHB induces antidepressant effects in an animal model of depression; however, it is unclear whether BHB specifically mediates these actions in the brain. Thus, we administered BHB directly into the brain in a rodent model of depression using a chronic unpredictable stress (CUS) paradigm. ...
Tópico(s): Stress Responses and Cortisol
2020 - Wiley | Neuropsychopharmacology Reports
Anna O. Pinson, Azure L. Yarbrough, John M. Bush, Christian V. Cabanlong, Amal Shoeib, Bailey K. Jackson, Saki Fukuda, Jyoti Gogoi, William E. Fantegrossi, Keith R. McCain, Paul L. Prather, Ryoichi Fujiwara, Anna Radomińska‐Pandya,
AKB48 and its fluorinated derivative 5F-AKB48 are synthetic cannabinoids (SCs) which have caused hospitalizations and deaths in human users. Abuse of SCs is dangerous because users may mistake them for natural cannabis, which is generally considered to be unlikely to elicit adverse effects. The present studies were designed to investigate the in vitro oxidative metabolism of 5F-AKB48 by human microsomal fractions from different organs and sexes as well as recombinant human cytochrome P450s (P450s). ...
Tópico(s): Alcohol Consumption and Health Effects
2020 - Elsevier BV | Pharmacology Biochemistry and Behavior
Hanna J. Kulbeth, Saki Fukuda, Lisa K. Brents,
Chronic prenatal exposure to opioids often causes fetal opioid dependence that leads to neonatal opioid withdrawal syndrome (NOWS) shortly after delivery. Rat models of NOWS often require quantifying neonatal withdrawal behaviors using time-consuming, labor-intensive manual scoring methods. The goal of this study was to automate quantification of opioid withdrawal in neonatal rat pups. Accordingly, we used the animal behavior software Ethovision® XT to analyze archived videos of rat pups subjected ...
Tópico(s): Neonatal and fetal brain pathology
2021 - Elsevier BV | Neurotoxicology and Teratology
Ayaka Tobo, Masayuki Tobo, Takashi Nakakura, Masashi Ebara, Hideaki Tomura, Chihiro Mogi, Dong‐Soon Im, Naoya Murata, Atsushi Kuwabara, Saki Ito, Hayato Fukuda, Mitsuhiro Arisawa, Satoshi Shuto, Michio Nakaya, Hitoshi Kurose, Kōichi Sato, Fumikazu Okajima,
G protein-coupled receptor 4 (GPR4), previously proposed as the receptor for sphingosylphosphorylcholine, has recently been identified as the proton-sensing G protein-coupled receptor (GPCR) coupling to multiple intracellular signaling pathways, including the Gs protein/cAMP and G13 protein/Rho. In the present study, we characterized some imidazopyridine compounds as GPR4 modulators that modify GPR4 receptor function. In the cells that express proton-sensing GPCRs, including GPR4, OGR1, TDAG8, and ...
Tópico(s): Drug Transport and Resistance Mechanisms
2015 - Public Library of Science | PLoS ONE
Yumi Kanegae, Miho Terashima, Saki Kondo, Hiromitsu Fukuda, Aya Maekawa, Pei Zheng, Izumu Saito,
Tissue-/cancer-specific promoters for use in adenovirus vectors (AdVs) are valuable for elucidating specific gene functions and for use in gene therapy. However, low activity, non-specific expression and size limitations in the vector are always problems. Here, we developed a 'double-unit' AdV containing the Cre gene under the control of an α-fetoprotein promoter near the right end of its genome and bearing a compact 'excisional-expression' unit consisting of a target cDNA 'upstream' of a potent promoter ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2010 - Oxford University Press | Nucleic Acids Research
Yoshiaki Tomimori, Tsuyoshi Muto, Harukazu Fukami, Kayo Saito, Chika Horikawa, Nobuo Tsuruoka, Masayuki Saito, Namino Sugiura, Kyoko Yamashiro, Motoo Sumida, Saki Kakutani, Yoshiaki Fukuda,
An epicutaneous application of 2,4-dinitrofluorobenzene (DNFB) to a mouse ear caused a transient skin swelling, and the repetition of the challenge enlarged the contact dermatitis. The repeated challenge with DNFB also induced eosinophil infiltration on the application site. Administration of a chymase inhibitor significantly inhibited the ear swelling as well as eosinophil accumulation. An intradermal injection of human chymase to the mouse ear also elicited transient skin swelling and eosinophil ...
Tópico(s): Asthma and respiratory diseases
2002 - Elsevier BV | Laboratory Investigation
Yoshiaki Tomimori, Nobuo Tsuruoka, Harukazu Fukami, Kayo Saito, Chika Horikawa, Masayuki Saito, Tsuyoshi Muto, Namino Sugiura, Kyoko Yamashiro, Motoo Sumida, Saki Kakutani, Yoshiaki Fukuda,
Intradermal injection of human chymase (EC 3.4.21.39) into the mouse ear elicited an edematous skin reaction in a biphasic manner, with a transient reaction peaking at 1 hr, followed by a delayed response persisting for at least 24hr. The kinetics of this reaction was analogous to the biphasic skin reaction induced by ascaris extract in actively sensitized mice. A similarity between the two dermatitis models was also shown by histological analysis, i.e. accumulation of inflammatory cells was observed ...
Tópico(s): Asthma and respiratory diseases
2002 - Elsevier BV | Biochemical Pharmacology
Yoshiaki Tomimori, Tsuyoshi Muto, Harukazu Fukami, Kayo Saito, Chika Horikawa, Nobuo Tsuruoka, Kyoko Yamashiro, Masayuki Saito, Namino Sugiura, Motoo Sumida, Saki Kakutani, Yoshiaki Fukuda,
Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These results suggest that chymase released by mast cells may participate in local mast cell accumulation in a positive feedback fashion. Immunohistochemical analysis revealed that the intradermal injection of chymase ...
Tópico(s): Asthma and respiratory diseases
2002 - Elsevier BV | Biochemical and Biophysical Research Communications
Hayato Fukuda, Saki Ito, Kenji Watari, Chihiro Mogi, Mitsuhiro Arisawa, Fumikazu Okajima, Hitoshi Kurose, Satoshi Shuto,
GPR4, a pH-sensing G protein-coupled receptor, is highly expressed in endothelial cells and may be activated in myocardial infarction due the decreased tissue pH. We are interested in GPR4 antagonists as potential effective pharmacologic tools and/or drug leads for the treatment of myocardial infarction. We investigated the structure-activity relationship of a known GPR4 antagonist 1 as a lead compound to identify 3b as the first potent and selective GPR4 antagonist, whose effectiveness was demonstrated ...
Tópico(s): Receptor Mechanisms and Signaling
2016 - American Chemical Society | ACS Medicinal Chemistry Letters
Nobuhiko Fukuda, Saki Naito, Daiki Masukawa, Moemi Kaneda, Hiroshi Miyamoto, Takaya Abe, Yui Yamashita, Itaru Endo, Fumio Nakamura, Yoshio Goshima,
Oa1 is the casual gene for ocular albinism-1 in humans. The gene product OA1, alternatively designated as GPR143, belongs to G-protein coupled receptors. It has been reported that OA1 is a specific receptor for 3, 4-dihydroxy- L-phenylalanine (DOPA) in retinal pigmental epithelium where DOPA facilitates the pigmentation via OA1 stimulation. We have recently shown that OA1 mediates DOPA-induced depressor response in rat nucleus tractus solitarii. However, the distribution and function of OA1 in other ...
Tópico(s): Olfactory and Sensory Function Studies
2015 - Elsevier BV | Brain Research
Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Yu Masaki, Asahi Fukuda, Saki Hashimoto, Masayoshi Nakasako, Yuichi Ichikawa, Hitoshi Kurumizaka, Mitsuhiro Shimizu, Yayoi Inui, Sachihiro Matsunaga, Takayuki Kato, Keiichi Namba, K. Yamaguchi, Kazuo Kuwata, Hiroshi Kameda, Naoya Fukui, Yasushi Kawata, Takashi Kameshima, Yuki Takayama, Koji Yonekura, Masaki Yamamoto,
We have developed an experimental apparatus named KOTOBUKI-1 for use in the coherent x-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. The apparatus allows us to collect diffraction data for frozen-hydrated specimens at 66 K and provides an experimental environment to easily transfer frozen-hydrated specimens from liquid nitrogen storage to the specimen stage for x-ray exposure. Since 2012, the apparatus has been used in the single-shot ...
Tópico(s): X-ray Spectroscopy and Fluorescence Analysis
2015 - IOP Publishing | Journal of Physics B Atomic Molecular and Optical Physics
Marina Ichige, Erina Fukuda, Saki Miida, Jun‐ichiro Hattan, Norihiko Misawa, Shun Saito, Takahiro Fujimaki, Masaya Imoto, Kazutoshi Shindo,
Isoflavone glucosides (2'-hydroxy,5-methoxy genistein-7-O-glucoside (1), 2'-hydroxy genistein-7-O-gentibioside (2), 5-methoxy genistein-7-O-glucoside (3), 3',5-dimethoxy genistein-7-O-glucoside (4), 2'-hydroxy genistein-7-O-glucoside (5), genistein-7-O-gentibioside (6), 2'-hydroxy,5-methoxy genistein-4',7-O-diglucoside (7), and 2'-hydroxy genistein-4',7-O-diglucoside (8)) were isolated from the groundnut of Apios americana Medik. Their structures were elucidated on the basis of HR-ESI-MS and 1D- and 2D-NMR ...
Tópico(s): Coconut Research and Applications
2013 - American Chemical Society | Journal of Agricultural and Food Chemistry
Masashi Nakajima, Kaori Sakuishi, Saki Fukuda, Shinsuke Fujioka, Hideji Hashida,
Tópico(s): Viral Infections and Immunology Research
2008 - Oxford University Press | Clinical Infectious Diseases
Abstract Whistling has become more popular in recent years. “Whistling competitions” are being held in Japan since 2005. However, it can be difficult to teach some people how to whistle. One of the reasons is that theoretical information, including clear descriptions about how to whistle is scant. A better understanding of the principle would be beneficial to both the trainer and the trainee. In this study, the glottis opening area during whistling was measured via endoscopic imaging data. The amplitude ...
Tópico(s): Diverse Musicological Studies
2020 - Wiley | Electronics and Communications in Japan
Morié Ishida, Saki P. Arai, Norihiko Ohbayashi, Mitsunori Fukuda,
The small GTPase Rab27A is a crucial regulator of actin-based melanosome transport in melanocytes, and functionally defective Rab27A causes human Griscelli syndrome type 2, which is characterized by silvery hair. A GTPase-deficient, constitutively active Rab27A(Q78L) mutant has been shown to act as an inhibitor of melanosome transport and to induce perinuclear aggregation of melanosomes, but the molecular mechanism by which Rab27A(Q78L) inhibits melanosome transport remained to be determined. In ...
Tópico(s): Retinal Development and Disorders
2014 - Elsevier BV | Journal of Biological Chemistry
Asako Kukita, Kenbun Sone, Syuzo Kaneko, Eiryo Kawakami, Shinya Oki, Machiko Kojima, Miku Wada, Yusuke Toyohara, Yu Takahashi, Futaba Inoue, Saki Tanimoto, Ayumi Taguchi, Tomohiko Fukuda, Yuichiro Miyamoto, Michihiro Tanikawa, Mayuyo Mori‐Uchino, Tetsushi Tsuruga, Takayuki Iriyama, Yōko Matsumoto, Kazunori Nagasaka, Osamu Wada‐Hiraike, Katsutoshi Oda, Ryuji Hamamoto, Yutaka Osuga,
The histone methyltransferase SET domain-containing protein 8 (SETD8), which methylates histone H4 lysine 20 (H4K20) and non-histone proteins such as p53, plays key roles in human carcinogenesis. Our aim was to determine the involvement of SETD8 in endometrial cancer and its therapeutic potential and identify the downstream genes regulated by SETD8 via H4K20 methylation and the p53 signaling pathway. We examined the expression profile of SETD8 and evaluated whether SETD8 plays a critical role in the ...
Tópico(s): RNA modifications and cancer
2022 - Multidisciplinary Digital Publishing Institute | Cancers
Aya Maekawa, Pei Zheng, Mariko Suzuki, Hiromitsu Fukuda, Yohei Ono, Saki Kondo, Izumu Saito, Yumi Kanegae,
First-generation adenovirus vectors (FG AdVs) are widely used in basic studies and gene therapy. However, virus-associated (VA) RNAs that act as small-interference RNAs are indeed transcribed from the vector genome. These VA RNAs can trigger the innate immune response. Moreover, VA RNAs are processed to functional viral miRNAs and disturb the expressions of numerous cellular genes. Therefore, VA-deleted AdVs lacking VA RNA genes would be advantageous for basic studies, both in vitro and in vivo. Here, ...
Tópico(s): Viral gastroenteritis research and epidemiology
2013 - Nature Portfolio | Scientific Reports
Takashi Kato, Masaki Yamamoto, Yoshitaka Honda, Takashi Orimo, Izumi Sasaki, Kohei Murakami, Hiroaki Hemmi, Yuri Fukuda‐Ohta, Kyoichi Isono, Saki Takayama, Hidenori Nakamura, Yoshiro Otsuki, Toshiaki Miyamoto, Junko Takita, Takahiro Yasumi, Ryuta Nishikomori, Tadashi Matsubayashi, Kazushi Izawa, Tsuneyasu Kaisho,
Coatomer subunit alpha (COPA) syndrome, also known as autoinflammatory interstitial lung, joint, and kidney disease, is caused by heterozygous mutations in COPA. We identified a novel COPA variant in 4 patients in one family. We undertook this study to elucidate whether and how the variant causes manifestations of COPA syndrome by studying these 4 patients and by analyzing results from a gene-targeted mouse model.We performed whole-exome sequencing in 7 family members and measured the type I interferon ( ...
Tópico(s): Immune Response and Inflammation
2021 - Wiley | Arthritis & Rheumatology
Yuki Fukuhara, Naofumi Kamimura, Masahiro Nakajima, Shojiro Hishiyama, Hirofumi Hara, Daisuke Kasai, Yukiko Tsuji, Sachiko Narita-Yamada, Saki Nakamura, Y. Katano, Nobuyuki Fujita, Yoshihiro Katayama, Masao Fukuda, Shinya Kajita, Eiji Masai,
Bacterial genes for the degradation of major dilignols produced in lignifying xylem are expected to be useful tools for the structural modification of lignin in plants. For this purpose, we isolated pinZ involved in the conversion of pinoresinol from Sphingobium sp. strain SYK-6. pinZ showed 43–77% identity at amino acid level with bacterial NmrA-like proteins of unknown function, a subgroup of atypical short chain dehydrogenases/reductases, but revealed only 15–21% identity with plant pinoresinol/ ...
Tópico(s): Plant-derived Lignans Synthesis and Bioactivity
2012 - Elsevier BV | Enzyme and Microbial Technology
Whistling has become more popular in recent years. “Whistling competitions” are being held in Japan since 2005. However, it can be difficult to teach some people how to whistle. One of the reasons is that theoretical information, including clear descriptions about how to whistle is scant. A better understanding of the principle would be beneficial to both the trainer and the trainee. In the present study, the glottis opening area during whistling was measured via endoscopic imaging data. The amplitude ...
Tópico(s): Musicians’ Health and Performance
2019 - Institute of Electrical Engineers of Japan | IEEJ Transactions on Fundamentals and Materials
Mikio Mori, Saki Fukuda, Kotaro Ishiguro, Saran Sukplang,
Whistling competitions have been held regularly in Japan in recent years, and some prize-winning whistlers have established music classrooms to teach musical whistling. However, in our survey, one-third of people could not whistle. Our work studied the principles of sound production in human whistling, using physical models of the human vocal tract. The models were obtained from an X-ray computed tomography (CT) image of the vocal tract during whistling. However, it is hard to explain to the person ...
Tópico(s): Global trade, sustainability, and social impact
2019 - Institute of Electrical Engineers of Japan | IEEJ Transactions on Fundamentals and Materials
Yasushi Ichikawa, Eri Oota, Susumu Odajima, Masayuki Kintsu, Saki Todo, Kimikazu Takeuchi, Yuki Yamauchi, Hiroaki Shiraki, Kentaro Yamashita, Terunobu Fukuda, Eriko Hisamatsu, Ken‐ichi Hirata, Hidekazu Tanaka,
The efficacy of a therapy for patients with transthyretin amyloid cardiomyopathy (ATTR-CM) has not been proven, but tafamidis has been associated with favorable outcomes. However, echocardiographic details of the association of tafamidis with cardiac morphology remain undetermined. Moreover, whether the efficacy of tafamidis varies with the degree of cardiac involvement remains unknown. Using echocardiography, this study investigated the impact of tafamidis on the cardiac morphology of patients with ...
Tópico(s): Peptidase Inhibition and Analysis
2023 - Japanese Circulation Society | Circulation Journal
Takashi Okitsu, Hikaru Nakahigashi, Ryosuke Sugihara, Ikki Fukuda, Saki Tsuji, Yasuko In, Akimori Wada,
Iodocyclization of silyl group-substituted homopropargylic carbamates and amides proceeded via 6-exo-dig mode to afford 6-vinylene-4,5-dihydro-1,3-oxazines in moderate to quantitative yields. This is the first report for silyl group-solely directed iodocyclization of alkynes utilizing the β-silyl effect. Under these mild reaction conditions, various functionalities such as secondary alcohol, acetal, urea, and sulfide were tolerated.
Tópico(s): Catalytic Alkyne Reactions
2018 - Wiley | Chemistry - A European Journal
Yui Fukuda, Takumi Kikumura, Saki Sakoda, Genki Ikeda, Yuki Nakamura, Masakazu Dojyo, Yasunori Yamada, Takeshi Hanamoto,
A practical synthesis of (Z)-2-bromo-2-CF3-vinyl phenyl sulfide from 2-CF3-vinyl phenyl sulfide was achieved using bromine (Br2) in the presence of LiBr and LiOAc in AcOH in one flask. This method affords a stereodefined product in high yield under mild conditions. The synthetic application of the product was briefly examined, providing a wide range of Sonogashira cross-coupling products using terminal acetylenes as a coupling partner.
Tópico(s): Chemical Synthesis and Analysis
2019 - Thieme Medical Publishers (Germany) | Synlett
Mitsuyoshi Fukuda, Takanori Kanazawa, Shingo Iioka, Takayuki Oguma, Ryohei Iwasa, Saki Masuoka, Naoto Suzuki, Yasuhiro Kosuge, Toyofumi Suzuki,
This study aimed to determine the effect of intranasal dosing speed and administrating volume of nose-to-brain delivery on candidates for peptide drugs (molecular weight ca. 1–10 kDa). Using inulin as the model molecule of a peptide drug, intranasal administration by cannulation from the airway side through the esophagus was tested in mice. This was done to determine the quantitative distribution levels of the drug in the brain and cerebral spinal fluid (CSF). Distribution levels were increased with ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2021 - Elsevier BV | Journal of Controlled Release
Yukiko Tsuji, Ruben Vanholme, Yuki Tobimatsu, Yasuyuki Ishikawa, Clifton E. Foster, Naofumi Kamimura, Shojiro Hishiyama, Saki Hashimoto, Amiu Shino, Hirofumi Hara, Kanna Sato‐Izawa, Paula Oyarce, Geert Goeminne, Kris Morreel, Jun Kikuchi, Toshiyuki Takano, Masao Fukuda, Yoshihiro Katayama, Wout Boerjan, John Ralph, Eiji Masai, Shinya Kajita,
Summary Bacteria‐derived enzymes that can modify specific lignin substructures are potential targets to engineer plants for better biomass processability. The Gram‐negative bacterium Sphingobium sp. SYK ‐6 possesses a Cα‐dehydrogenase (LigD) enzyme that has been shown to oxidize the α‐hydroxy functionalities in β–O–4‐linked dimers into α‐keto analogues that are more chemically labile. Here, we show that recombinant LigD can oxidize an even wider range of β–O–4‐linked dimers and oligomers, including ...
Tópico(s): Biochemical and biochemical processes
2015 - Wiley | Plant Biotechnology Journal
Akihiko Miura, Takehiko Yamanashi, Naofumi Kajitani, Saki Fukuda, Kyohei Tsunetomi, Ryoichi Matsuo, Tsuyoshi Nishiguchi, Shenghong Pu, Yumeto Nakada, Yukihiko Shirayama, Ken Watanabe, Koichi Kaneko, Masaaki Iwata,
Tópico(s): Adipose Tissue and Metabolism
2025 - Tottori University | Yonago acta medica
Julia Tobacyk, Saki Fukuda, Brian J. Parks, Michael D. Berquist, Lisa K. Brents,
Abstract ID 90377 Poster Board 078 During the 12-month period ending in April 2021, opioid-related deaths, primarily from fentanyl, reached an all-time high in the US, affecting up to 100,000 people annually. People undergoing medication-assisted treatment for opioid use disorder (OUD ), including pregnant women, often relapse to fentanyl use. The gold standard treatment for OUD during pregnancy is buprenorphine (BUP) , which improves maternal-fetal outcomes but causes neonatal opioid withdrawal syndrome (NOWS) ...
Tópico(s): Diet and metabolism studies
2024 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Yosuke Toya, Waku Hatta, Tomohiro Shimada, Tamotsu Matsuhashi, Takeharu Shiroki, Yu Sasaki, Tetsuya Tatsuta, Jun Nakamura, Norihiro Hanabata, Yohei Horikawa, Ko Nagino, Tomoyuki Koike, Atsushi Masamune, Yoshihiro Harada, Tetsuya Ohira, Katsunori Iijima, Yasuhiko Abe, Takuto Hikichi, Shohei Igarashi, Saki Fushimi, Hiroaki Takeda, Shinsaku Fukuda, Takayuki Matsumoto,
Clinical outcomes of endoscopic submucosal dissection (ESD) for esophageal squamous cell carcinoma (ESCC) with esophageal varices (EVs) are obscure. We aimed to elucidate the clinical outcomes of ESD for ESCC with EVs in a multicenter, retrospective study.
Tópico(s): Gastric Cancer Management and Outcomes
2023 - Wiley | Digestive Endoscopy
The principle of sound production in human whistling is not well-known. A good understanding of the principle would be very beneficial to both the trainer and the trainee. We previously built a physical model of a human vocal tract on whistling developed from a CT image of the vocal tract during whistling, and demonstrate that the whistle pitch increased by narrowing the oral cavity. However, some whistle performers change the whistle pitch without almost changing the oral cavity shape. No studies ...
Tópico(s): Design Education and Practice
2020 - Institute of Electrical Engineers of Japan | IEEJ Transactions on Fundamentals and Materials