Produção Nacional
Revisado por pares
Gustavo Jorge dos Santos, Sandra Mara Ferreira, Fernanda Ortis, Luiz Fernando de Rezende, Chengyang Li, Ali Naji, Everardo M. Carneiro, Klaus H. Kaestner, Antônio C. Boschero,
Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) functions both in regulation of insulin secretion and neurotransmitter release through common downstream mediators. Therefore, we hypothesized that pancreatic ß-cells acquire and store the information contained in calcium pulses as a form of "metabolic memory", just as neurons store cognitive information. To test this hypothesis, we developed a novel paradigm of pulsed exposure of ß-cells to intervals of high glucose, followed by a 24-h consolidation ...
Tópico(s): Receptor Mechanisms and Signaling
2014 - Elsevier BV | Molecular Metabolism
Produção Nacional
Revisado por pares
P Brandimarti, José Maria Costa-Júnior, Sandra Mara Ferreira, André Otávio Peres Protzek, Gustavo Jorge dos Santos, Everardo M. Carneiro, Antônio C. Boschero, Luiz Fernando de Rezende,
Insulin clearance plays a major role in glucose homeostasis and insulin sensitivity in physiological and/or pathological conditions, such as obesity-induced type 2 diabetes as well as diet-induced obesity. The aim of the present work was to evaluate cafeteria diet-induced obesity-induced changes in insulin clearance and to explain the mechanisms underlying these possible changes. Female Swiss mice were fed either a standard chow diet (CTL) or a cafeteria diet (CAF) for 8 weeks, after which we performed ...
Tópico(s): Pancreatic function and diabetes
2013 - Bioscientifica | Journal of Endocrinology
Produção Nacional
Revisado por pares
Flávia M. Paula, Sandra Mara Ferreira, Antônio C. Boschero, Kleber L.A. Souza,
Peroxiredoxins are a family of six antioxidant enzymes (PRDX1-6), and may be an alternative system for the pancreatic beta cells to cope with oxidative stress. This study investigated whether the main diabetogenic pro-inflammatory cytokines or the anti-inflammatory cytokine IL-4 modulate PRDXs levels and putative intracellular pathways important for this process in the insulin-producing RINm5F cells. RINm5F cells expressed significant amounts of PRDX1, PRDX3 and PRDX6 enzymes. Only PRDX6 was modulated ...
Tópico(s): Heat shock proteins research
2013 - Elsevier BV | Molecular and Cellular Endocrinology
Produção Nacional
Revisado por pares
José Maria Costa Júnior, Morgana Rejane Rabelo Rosa, André Otávio Peres Protzek, Flávia MM De Paula, Sandra Mara Ferreira, Luiz Fernando de Rezende, Emerielle C. Vanzela, Cláudio C. Zoppi, Leonardo R. Silveira, Ísis do Carmo Kettelhut, Antônio C. Boschero, Camila Aparecida Machado de Oliveira, Everardo M. Carneiro,
Tópico(s): Adipose Tissue and Metabolism
2015 - Springer Science+Business Media | Amino Acids
Produção Nacional
Revisado por pares
André Otávio Peres Protzek, Luiz Fernando de Rezende, José Maria Costa-Júnior, Sandra Mara Ferreira, Ana Paula Gameiro Cappelli, Flávia M. Paula, Jane Cristina de Souza, Mirian Ayumi Kurauti, Everardo M. Carneiro, Alex Rafacho, Antônio C. Boschero,
Glucocorticoid treatment induces insulin resistance (IR), which is counteracted by a compensatory hyperinsulinemia, due to increased pancreatic β-cell function. There is evidence for also reduced hepatic insulin clearance, but whether this correlates with altered activity of insulin-degrading enzyme (IDE) in the liver, is not fully understood. Here, we investigated whether hyperinsulinemia, in glucocorticoid-treated rodents, is associated with any alteration in the insulin clearance and activity of ...
Tópico(s): Regulation of Appetite and Obesity
2015 - Elsevier BV | The Journal of Steroid Biochemistry and Molecular Biology
Produção Nacional
Revisado por pares
José Maria Costa-Júnior, Sandra Mara Ferreira, Mirian Ayumi Kurauti, Diana L. Bernstein, Elena G. Ruano, Vasumathi Kameswaran, Jonathan Schug, Ricardo de Freitas Dias, Cláudio C. Zoppi, Antônio C. Boschero, Camila Aparecida Machado de Oliveira, Gustavo Jorge dos Santos, Everardo M. Carneiro, Klaus H. Kaestner,
Epigenetic regulation is considered the main molecular mechanism underlying the developmental origin of health and disease's (DOHAD) hypothesis. Previous studies that have investigated the role of paternal exercise on the metabolic health of the offspring did not control for the amount and intensity of the training or possible effects of adaptation to exercise and produced conflicting results regarding the benefits of parental exercise to the next generation. We employed a precisely regulated exercise ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Produção Nacional
Revisado por pares
Mirian Ayumi Kurauti, Ricardo de Freitas Dias, Sandra Mara Ferreira, Jean Franciesco Vettorazzi, Tarlliza R. Nardelli, Hygor N. Araújo, Gustavo Jorge dos Santos, Everardo M. Carneiro, Antônio C. Boschero, Luiz Fernando de Rezende, José Maria Costa-Júnior,
The effects of exercise on insulin clearance and IDE expression are not yet fully elucidated. Here, we have explored the effect of acute exercise on insulin clearance and IDE expression in lean mice. Male Swiss mice were subjected to a single bout of exercise on a speed/angle controlled treadmill for 3-h at approximately 60-70% of maximum oxygen consumption. As expected, acute exercise reduced glycemia and insulinemia, and increased insulin tolerance. The activity of AMPK-ACC, but not of IR-Akt, ...
Tópico(s): Apelin-related biomedical research
2016 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Mirian Ayumi Kurauti, José Maria Costa-Júnior, Sandra Mara Ferreira, Gustavo Jorge dos Santos, André Otávio Peres Protzek, Tarlliza R. Nardelli, Luiz Fernando de Rezende, Antônio C. Boschero,
The aim of this study was to investigate the insulin clearance in diet-induced obese (DIO) mice submitted to acute endurance exercise (3h of treadmill exercise at 60-70% VO2max). Glucose-stimulated insulin secretion in isolated islets; ipGTT; ipITT; ipPTT; in vivo insulin clearance; protein expression in liver, skeletal muscle, and adipose tissue (insulin degrading enzyme (IDE), insulin receptor subunitβ(IRβ), phospho-Akt (p-Akt) and phospho-AMPK (p-AMPK)), and the activity of IDE in the liver and skeletal ...
Tópico(s): Metabolism, Diabetes, and Cancer
2016 - Bioscientifica | Journal of Endocrinology
Produção Nacional
Revisado por pares
Mirian Ayumi Kurauti, José Maria Costa-Júnior, Sandra Mara Ferreira, Gustavo Jorge dos Santos, Carlos H. Sponton, Everardo M. Carneiro, Guilherme Defante Telles, Mara Patrícia Traina Chacon‐Mikahil, Cláudia Regina Cavaglieri, Luiz Fernando de Rezende, Antônio C. Boschero,
Abstract Impairment of the insulin-degrading enzyme (IDE) is associated with obesity and type 2 diabetes mellitus (T2DM). Here, we used 4-mo-old male C57BL/6 interleukin-6 (IL-6) knockout mice (KO) to investigate the role of this cytokine on IDE expression and activity. IL-6 KO mice displayed lower insulin clearance in the liver and skeletal muscle, compared with wild type (WT), due to reduced IDE expression and activity. We also observed that after 3-h incubation, IL-6, 50 and 100 ng ml −1 , increased ...
Tópico(s): Exercise and Physiological Responses
2017 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Jean Franciesco Vettorazzi, Mirian Ayumi Kurauti, Gabriela Moreira Soares, Patrícia Cristine Borck, Sandra Mara Ferreira, Renato Chaves Souto Branco, Luciana de Souza Lima Michelone, Antônio C. Boschero, José Maria Costa, Everardo M. Carneiro,
Disruption of insulin secretion and clearance both contribute to obesity-induced hyperinsulinemia, though reduced insulin clearance seems to be the main factor. The liver is the major site for insulin degradation, a process mainly coordinated by the insulin-degrading enzyme (IDE). The beneficial effects of taurine conjugated bile acid (TUDCA) on insulin secretion as well as insulin sensitivity have been recently described. However, the possible role of TUDCA in insulin clearance had not yet been explored. ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2017 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Mirian Ayumi Kurauti, Sandra Mara Ferreira, Gabriela Moreira Soares, Jean Franciesco Vettorazzi, Everardo M. Carneiro, Antônio C. Boschero, José Maria Costa-Júnior,
Human life expectancy is increasing faster lately and, consequently, the number of patients with age-related diseases such as type 2 diabetes (T2D) is rising every year. Cases of hyperinsulinemia have been extensively reported in elderly subjects and this alteration in blood insulin concentration is postulated to be a cause of insulin resistance, which in some cases triggers T2D onset. Thus, it is important to know the underlying mechanisms of age-dependent hyperinsulinemia to find new strategies ...
Tópico(s): Regulation of Appetite and Obesity
2018 - Wiley | Journal of Cellular Physiology
Produção Nacional
Revisado por pares
José Maria Costa-Júnior, Sandra Mara Ferreira, André Otávio Peres Protzek, Gustavo Jorge dos Santos, Ana Paula Gameiro Cappelli, Leonardo R. Silveira, Cláudio C. Zoppi, Camila Aparecida Machado de Oliveira, Antônio C. Boschero, Everardo M. Carneiro, Luiz Fernando de Rezende,
Introduction Endurance training improves peripheral insulin sensitivity in the liver and the skeletal muscle, but the mechanism for this effect is poorly understood. Recently, it was proposed that insulin clearance plays a major role in both glucose homeostasis and insulin sensitivity. Therefore, our goal was to determine the mechanism by which endurance training improves insulin sensitivity and how it regulates insulin clearance in mice. Methods Mice were treadmill-trained for 4 weeks at 70–80% ...
Tópico(s): Pancreatic function and diabetes
2015 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Francine Pereira de Carvalho, Thaís Ludmilla Moretto, Izabelle Dias Benfato, Marcela Barthichoto, Sandra Mara Ferreira, José Maria Costa-Júnior, Camila Aparecida Machado de Oliveira,
To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57Bl/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral ...
Tópico(s): Adipokines, Inflammation, and Metabolic Diseases
2018 - Portland Press | Bioscience Reports
Produção Nacional
Revisado por pares
Ana Luísa Valente, Cláudia Delgado, Cláudia Moreira, Sandra Mara Bessa Ferreira, Thomas Dellinger, Miguel Â. A. Pinheiro de Carvalho, Graça Costa,
The helminth fauna of pelagic-stage loggerhead sea turtles, Caretta caretta, is still poorly known. Here, we describe the helminth-component community of healthy, free-ranging juvenile loggerhead sea turtles captured in the waters around Madeira Island, Portugal. Fifty-seven were used in this study. The esophagus, stomach, intestine, liver, gallbladder, spleen, kidneys, trachea, bronchi, urinary bladder, heart, left and right aortas, and coelomic cavity were macroscopically inspected; organs and ...
Tópico(s): Bird parasitology and diseases
2009 - American Society of Parasitologists | Journal of Parasitology
Produção Nacional
Revisado por pares
Ana Augusta Salvador, Rossana Podestá, Jane Mara Block, Sandra Regina Salvador Ferreira,
Pecan nut is a dried fruit, appreciated worldwide because of its particularly pleasant flavor and its wealth of healthy bioactive components. The nut oil is rich in monounsaturated fatty acids and vitamin E, currently recognized as antioxidant agents. The oil extraction from the pecan nut cake, an industrial byproduct from oil production, is an alternative to value the industrial application of the pecan nut. In the present work, different extraction processes, low-pressure methods (LPE) and supercritical ...
Tópico(s): Bee Products Chemical Analysis
2016 - Elsevier BV | The Journal of Supercritical Fluids
Produção Nacional
Revisado por pares
Guilherme Galvarros Bueno Lôbo Ribeiro, Reginaldo Ramos De Lima, Cláudia Emília Vieira Wiezel, Luzitano Brandão Ferreira, Sandra Mara Bispo Sousa, Dulce Maria Sucena da Rocha, Maria do Carmo Tomitão Canas, Juliana Nardelli‐Costa, Maria de Nazaré Klautau‐Guimarães, Aguinaldo Luíz Simões, Silviene Fabiana de Oliveira,
Abstract The genetic constitution of Afro‐derived Brazilian populations is barely studied. To improve that knowledge, we investigated the Alu YAP element and five Y‐chromosome STRs (DYS19, DYS390, DYS391, DYS392, and DYS393) to estimate ethnic male contribution in the constitution of four Brazilian quilombos remnants: Mocambo , Rio das Rãs , Kalunga , and Riacho de Sacutiaba . Results indicated significant differences among communities, corroborating historical information about the Brazilian settlement. ...
Tópico(s): Genetic diversity and population structure
2009 - Wiley | American Journal of Human Biology
Produção Nacional
Revisado por pares
Catharina de Barros Pimentel Villaça, Carolina Cavalcante de Paula, Caroline Cruz de Oliveira, Eloisa Aparecida Vilas‐Boas, Junia Carolina Santos-Silva, Sérgio Ferreira de Oliveira, Fernando Abdulkader, Sandra Mara Ferreira, Fernanda Ortis,
What is the central question of this study? Type 1 diabetes mellitus (T1D) leads to hyperglycaemia owing to pancreatic β-cell destruction by the immune system. Physical exercise has been shown to have potentially beneficial protective roles against cytokine-induced pancreatic β-cell death, but its benefits are yet to be proved and should be understood better, especially in the islet environment. What is the main finding and its importance? Physical exercise protects against β-cell loss in a well- ...
Tópico(s): Diabetes Management and Research
2021 - Wiley | Experimental Physiology
Produção Nacional
Revisado por pares
Sandra Mara Ferreira, Gustavo Jorge dos Santos, Luiz Fernando de Rezende, Luciana Mateus Gonçalves, Junia Carolina Santos-Silva, Carolina L. Bigarella, Everardo M. Carneiro, Sara T.O. Saad, Antônio C. Boschero, Helena C.L. Barbosa,
ARHGAP21 is a Rho GTPase-activating protein (RhoGAP) that associates with many proteins and modulates several cellular functions, including actin cytoskeleton rearrangement in different tissues. However, it is unknown whether ARHGAP21 is expressed in pancreatic beta cells and its function in these cells. Herein, we assess the participation of ARHGAP21 in insulin secretion. Neonatal mice were treated with anti-sense oligonucleotide against ARHGAP21 (AS) for 2 days, resulting in a reduction of the protein' ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2015 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Izabelle Dias Benfato, Thaís Ludmilla Moretto, Francine Pereira de Carvalho, Marcela Barthichoto, Sandra Mara Ferreira, José Maria Costa Júnior, Mariana Cruz Lazzarin, Flávia de Oliveira, Carolina S. Martínez, Carolina Prado de França Carvalho, Camila Aparecida Machado de Oliveira,
What is the central question of this study? Is the initial decline of spontaneous physical activity (SPA) in mice related to impaired insulin and leptin signalling or brain-derived neurotrophic factor expression in the hypothalamus? What is the main finding and its importance? We showed that SPA started to decline at an early stage, concomitantly with an impairment of hypothalamic leptin signalling. Consequently, energy expenditure decreased and glucose tolerance worsened. Our results demonstrate ...
Tópico(s): Obesity, Physical Activity, Diet
2017 - Wiley | Experimental Physiology
Produção Nacional
Revisado por pares
Ana Cristina Pinheiro do Prado, Helen Silvestre da Silva, Sheila Mello da Silveira, Pedro Luiz Manique Barreto, Cleide Rosana Werneck Vieira, Marcelo Maraschin, Sandra Regina Salvador Ferreira, Jane Mara Block,
In this study, the effect of the extraction processes (infusion, infusion followed by spray drying, ethanol extraction and supercritical extraction) on the total content and profile of phenolic compounds, antioxidant and antimicrobial activities of extracts of pecan nut shell were studied. The extract obtained through infusion followed by atomization in a spray dryer showed significantly higher (p < 0.05) contents of total phenolic compounds (590.78 mg GAE/g) and condensed tannins (412.10 mg CE/g), and ...
Tópico(s): Essential Oils and Antimicrobial Activity
2013 - Elsevier BV | Industrial Crops and Products
Produção Nacional
Revisado por pares
Richard Tello, Sandra Mara Torres Müller, André Ferreira, Teodiano Bastos-Filho,
Introduction The main idea of a traditional Steady State Visually Evoked Potentials (SSVEP)-BCI is the activation of commands through gaze control. For this purpose, the retina of the eye is excited by a stimulus at a certain frequency. Several studies have shown effects related to different kind of stimuli, frequencies, window lengths, techniques of feature extraction and even classification. So far, none of the previous studies has performed a comparison of performance of stimuli colors through ...
Tópico(s): Neural dynamics and brain function
2015 - Springer Nature | Research on Biomedical Engineering
Produção Nacional
Revisado por pares
Gerson Lopes Teixeira, Laércio Galvão Maciel, Simone Mazzutti, Cintia B. Gonçalves, Sandra Regina Salvador Ferreira, Jane Mara Block,
The Amazonian tree Pentaclethra macroloba (pracaxi) has pod-shaped fruits with flattened seeds mostly composed of lipids, carbohydrates, and proteins, besides secondary compounds, which provide potential use by many industries. This study evaluated the supercritical carbon dioxide (SC-CO2) extraction of pracaxi seed oil (PSO) at different conditions of pressure (200–300 bar) and temperature (40–60 °C). The PSO was assessed for its fatty acids and triacylglycerols (TAG) compositions, total phenolic content ...
Tópico(s): Plant biochemistry and biosynthesis
2020 - Elsevier BV | Biocatalysis and Agricultural Biotechnology
Produção Nacional
Revisado por pares
Ágatha Nogueira Previdelli, Samantha Caesar de Andrade, Milena Monfort Pires, Sandra Roberta G. Ferreira, Regina Mara Fisberg, Dirce Maria Lobo Marchioni,
O Índice de Qualidade da Dieta Revisado é um indicador de qualidade da dieta desenvolvido consoante com as recomendações nutricionais vigentes. Os dados dietéticos foram provenientes do estudo de base-populacional, Inquérito de Saúde e Alimentação (ISA)-Capital-2003. O Índice contém 12 componentes, sendo nove fundamentados nos grupos de alimentos do Guia Alimentar Brasileiro de 2006, cujas porções diárias são expressas em densidade energética; dois nutrientes (sódio e gordura saturada); e Gord_AA ( ...
Tópico(s): Consumer Attitudes and Food Labeling
2011 - UNIVERSIDADE DE SÃO PAULO | Revista de Saúde Pública
Produção Nacional
Revisado por pares
Ágatha Nogueira Previdelli, Samantha Caesar de Andrade, Milena Monfort Pires, Sandra Roberta G. Ferreira, Regina Mara Fisberg, Dirce Maria Lobo Marchioni,
O Índice de Qualidade da Dieta Revisado é um indicador de qualidade da dieta desenvolvido consoante com as recomendações nutricionais vigentes. Os dados dietéticos foram provenientes do estudo de base-populacional, Inquérito de Saúde e Alimentação (ISA)-Capital-2003. O Índice contém 12 componentes, sendo nove fundamentados nos grupos de alimentos do Guia Alimentar Brasileiro de 2006, cujas porções diárias são expressas em densidade energética; dois nutrientes (sódio e gordura saturada); e Gord_AA ( ...
Tópico(s): Obesity, Physical Activity, Diet
2011 - UNIVERSIDADE DE SÃO PAULO | Revista de Saúde Pública
Produção Nacional
Revisado por pares
Gabriela Polmann, Vinícius Badia, Renan Danielski, Sandra Regina Salvador Ferreira, Jane Mara Block,
The purpose of this review is to demonstrate how the unexplored nuts native to Brazil can contribute positively to biodiversity and human nutrition. This review presents data on the composition of macronutrients, micronutrients, and bioactive compounds of non-conventional nuts native from Brazil (baru, chichá, gurguéia, monguba, pracaxi and sapucaia). The knowledge about these underutilized raw materials is important to stimulate their production, commercialization and consumption, as well as generate ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2021 - Elsevier BV | Future Foods
Produção Nacional
Revisado por pares
Gerson Lopes Teixeira, Laércio Galvão Maciel, Simone Mazzutti, Rafaela Cristina Turola Barbi, Rosemary Hoffmann Ribani, Sandra Regina Salvador Ferreira, Jane Mara Block,
Pachira aquatica Aubl. (munguba) seeds represent a rich source of lipids and biocompounds with potential for sustainable exploitation. This work evaluated the feasibility of using supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) to obtain a lipid-rich fraction and extracts rich in biocompounds from munguba seeds. The SFE at 30 MPa/60 °C/120 min was the best condition for munguba seed oil (MSO) extraction (52 g 100 g−1), providing 95% extraction efficiency compared to Soxhlet ...
Tópico(s): Polysaccharides Composition and Applications
2021 - Elsevier BV | Journal of Cleaner Production
Produção Nacional
Revisado por pares
Gabriela Polmann, Vinícius Badia, Renan Danielski, Sandra Regina Salvador Ferreira, Jane Mara Block,
This review provides information on nutritional characteristics, extraction techniques, bioactive compounds, bioavailability and bioaccessibility through in vitro and in vivo assays on nuts and food products obtained from walnuts, such as almonds, walnuts, cashew nuts, pistachios, hazelnuts, walnuts, walnuts, macadamia nuts, Brazil nuts, pine nuts and peanuts. The influence of the consumption of these nuts on human health was carried out through a meta-analysis. Data meta-analysis indicated that ...
Tópico(s): Heavy Metals in Plants
2022 - Taylor & Francis | Food Reviews International
Produção Nacional
Revisado por pares
Luana dos Santos, Jonathan Pena Castro, Carine Mendonça Francisco, Marcelo Ricardo Vicari, Mara Almeida, Leonardo Gusso Goll, Sandra Morelli, Roberto Ferreira Artoni,
B chromosomes, also known as supernumerary or accessory chromosomes, are additional chromosomes over the standard complement found in various groups of plants and animals. We investigated the presence of, and characterized, supernumerary microchromosomes in Astyanax goyacensis using classical and molecular cytogenetic methods.Three specimens possessed 2n = 50 chromosomes (8m + 26sm + 8st + 8a), and two specimens contained 1 to 9 additional B microchromosomes varying intra- and inter-individually. Chromosome ...
Tópico(s): Fish Biology and Ecology Studies
2013 - BioMed Central | Molecular Cytogenetics
Produção Nacional
Revisado por pares
Robson Barth, Carolina Ruoso, Sandra Mara Ferreira, Francieli Caroline de Ramos, Fernanda Barbosa Lima, Antônio C. Boschero, Gustavo Jorge dos Santos,
Regardless of the etiology, any type of DM presents a reduction of insulin-secreting cell mass, so it is important to investigate pathways that induce the increase of this cell mass.Based on the fact that (1) HNF4α is crucial for β-cell proliferation, (2) DEX-induced IR promotes β-cell mass expansion, and (3) the stimulation of β-cell mass expansion may be an important target for DM therapies, we aimed to investigate whether DEX-induced proliferation of β pancreatic cells is dependent on HNF4α.We used ...
Tópico(s): Diabetes Management and Research
2021 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Flávio José Sambatti Pieralisi, Mônica Rossi Rodrigues, V. G. Segura, Sandra Mara Maciel, F. B. A. Ferreira, José Eduardo García, Regina Célia Poli-Frederico,
Aim. The aim of the present paper was to evaluate the genotypic diversity of S. mutans in caries-free and caries-active preschool children in Brazil. Design. Twenty-eight preschool children were examined regarding caries experience by the dmft index. DNA from 280 isolates of S. mutans was extracted. S. mutans evaluated using to the PCR method, with primers for the glucosyltransferase gene. The genetic diversity of S. mutans isolates was analyzed by arbitrary primed-PCR (AP-PCR) reactions. The differences ...
Tópico(s): Gut microbiota and health
2009 - Hindawi Publishing Corporation | International Journal of Dentistry