Hiromichi OHTSUKA, Narumi Fukunaga, Hidefumi Hara, Shigeo Fukuda, Tomohito Hayashi, Fumio Hoshi, Tomoo YOSHINO, Masateru Koiwa, Seiichi Kawamura,
To clarify the cellular immune condition in Japanese Black (JB) calves with a weak syndrome, peripheral leukocyte populations were analyzed by flow cytometry. Twenty JB calves were divided into two groups based on clinical observations; one group of calves was weak, because they had experienced an onset of diarrhea within 3 days after birth and needed treatment (Group 1 ;n=10), and the other group of calves was healthy (Group 2; n=10). With regard to leukocyte populations, CD8(+) cells and gamma delta ...
Tópico(s): Toxin Mechanisms and Immunotoxins
2003 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Kenji Wada, Yayoi Hashiba, Hiromichi OHTSUKA, Masayuki Kohiruimaki, Machiko MASUI, Seiichi Kawamura, Hiroshi Endô, Yoshimi Ogata,
The effects of mycotoxins on mitogen-stimulated proliferation of bovine peripheral blood mononuclear cells (PBMCs) were investigated. Aflatoxin B(1) (AFB(1)), deoxynivalenol (DON) and zearalenone (ZEN) were added to cultures of PBMCs, and the proliferation responses were measured using MTT bioassays. Suppression of the proliferation of calf PBMCs by AFB(1) and DON was significantly stronger than that of cow PBMCs, whereas there were no differences in suppressive effects on PBMCs from Holstein and Japanese ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2008 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Maiko ONO, Yumi Saruyama, Machiko Mukai, Masayuki Kohiruimaki, Seiichi Kawamura,
ABSTRACT Japanese black (JB) calves have greater susceptibility to infectious diseases compared to Holstein (Hol) calves. In order to clarify the differences in cellular immune status between JB and Hol calves, the leukocyte population and lymphocyte proliferative ability were analyzed. In total 200 healthy calves, 1 day to 14 weeks of age, were examined: 105 JB and 95 Hol calves. Lower numbers in peripheral blood and percentage in peripheral blood mononuclear cells of CD3 + TcR1‐N12 + T cells and major ...
Tópico(s): T-cell and B-cell Immunology
2011 - Wiley | Animal Science Journal
Hiromichi OHTSUKA, Atsuya OGATA, Nobuhiro Terasaki, Masateru Koiwa, Seiichi Kawamura,
In this study, we investigated whether ozonated autohemoadministration (OAHA) influences leukocyte populations in cows with clinical inflammatory disease. Eleven cows with inflammatory disease (Inflammatory Group) and three healthy cows (Control Group) were used for this study. The CD4+/CD8+ ratio in the Inflammatory Group increased significantly compared to that in the Control Group 3 to 4 days after OAHA treatment. In the Inflammatory Group, the number of CD14+ cells decreased gradually after OAHA, ...
Tópico(s): Reproductive Physiology in Livestock
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Ken-ichi SHIBANO, Seiichi Kawamura,
Blood samples were taken from eight multiparous cows at a dairy farm on eight occasions between the prepartum period and peak lactation to study the serum concentrations of amino acids and biochemical constituents. The cows were classified as having either severe hepatic lipidosis (HL) or non-hepatic lipidosis (non-HL) according to their clinical condition after calving and changes in serum biochemical parameters. The serum concentrations of beta-hydroxybutyric acid were higher in the HL group than ...
Tópico(s): Ruminant Nutrition and Digestive Physiology
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Chika Watanabe, Masayuki Kohiruimaki, Takaaki ANDO, Daisaku WATANABE, Machiko MASUI, Tomohito Hayashi, Ryo Abe, Masateru Koiwa, Shigeru Sato, Seiichi Kawamura,
To clarify the relationship between cellular immune status and nutritive condition in periparturient dairy cows, feeding content, blood profiles, and immune condition were observed in cows from two dairy herds with different types of feed content. Immunological analyses such as leukocyte population and peripheral blood mononuclear cell (PBMC) mRNA of IFN-γ, TNF-α, IL-4, and IL-10, quantified by real-time RT-PCR were performed. With regard to feed content during dry periods, there were six cows in ...
Tópico(s): Cancer-related molecular mechanisms research
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Masateru Koiwa, Shigeo Fukuda, Yohei SATOH, Tomohito Hayashi, Fumio Hoshi, Tomoo YOSHINO, Seiichi Kawamura,
To clarify the cellular immune system in dairy cows with inflammatory diseases after calving, the leukocyte subsets were examined in Holstein dairy cows. Twenty Holstein cows reared in one herd, were used in this study. Nine cows (Group 1) experienced onset of mastitis or puerperal fever within 2 weeks after calving, and the other eleven cows remained healthy (Group 2) after calving. The numbers of CD3+, CD4+ and CD8+ cells tended to be lower in Group 1 than in Group 2 from the day of calving through ...
Tópico(s): Effects of Environmental Stressors on Livestock
2004 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Seiichi Higuchi, M. Fujimori, Fumio Hoshi, Seiichi Kawamura, Yoshio Yasuda,
The development of Babesia gibsoni in the salivary glands of larval Rhipicephalus sanguineus ticks was morphologically studied for 4 days of feeding of tick on rabbits. Babesia gibsoni showed two forms (sporont and sporozoite) in the budding process. Sporozoites were formed in the acinar cells of salivary glands and released from the hollow cytoplasmic area of these cells by day 4 of feeding. The morphology of B. gibsoni in the salivary glands of R. sanguineus shows a close similarity to those of ...
Tópico(s): Toxin Mechanisms and Immunotoxins
1995 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Masateru Koiwa, Atsushi HATSUGAYA, Katsunori Kudo, Fumio Hoshi, Noboru Itoh, Hiroshi Yokota, Hiroyuki Okada, Seiichi Kawamura,
To clarify the relationship between tumor necrosis factor (TNF) and insulin resistance in dairy cows affected with fatty liver, naturally occurring cases were investigated. The affected cows were classified into following three groups according to histopathologic findings of the liver: mild fat droplet deposition (group 1; n=11), severe fat droplet deposition (group 2; n=10), and cloudy swelling (group 3; n=8). Serum TNF activities in Group 2 (8.67 ± 2.16 U/ml) and Group 3 (11.65 ± 1.92 U/ml) were significantly ...
Tópico(s): Effects of Environmental Stressors on Livestock
2001 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Katsunori Kudo, Kazunori Mori, Fuminori Nagai, Atsushi HATSUGAYA, Motoshi Tajima, Keiji Tamura, Fumio Hoshi, Masateru Koiwa, Seiichi Kawamura,
Changes in the activities of serum cytokines and in acute phase response were observed in dairy cows with naturally occurring coliform mastitis. Seven cows with severe mastitis showed systemic and mammary inflammatory response throughout the observation period, and 11 cows with mild mastitis recovered and were able to be milked within 3 days of onset of mastitis. Serum interleukin (IL)-I and tumor necrosis factor (TNF) activities were higher in the severe group than in the mild group at the first ...
Tópico(s): Infant Nutrition and Health
2001 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Seiichi Higuchi, Hidehiro KURODA, Humio HOSHI, Seiichi Kawamura, Yoshio Yasuda,
Studies were made on the development of Babesia gibsoni in the midgut of the nymphal stage of the tick, Rhipicephalus sanguineus. Six hr after repletion, merozoites of B. gibsoni, free of erythrocytes, were observed in the midgut contents of the ticks. After that, within 24 hr, those merozoites were transformed into ring-forms which were relatively large ring 1-2 μm in diameter. Later, the ring forms developed into spherical forms which were somewhat elliptical in shape and 3-4 μm in diameter. Within ...
Tópico(s): Toxin Mechanisms and Immunotoxins
1999 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Morihiro HAMANA, Hiromichi OHTSUKA, M. Oikawa, Seiichi Kawamura,
In order to decipher the amino acid (AA) balance in the dairy cattle with left displaced abomasum (LDA), blood samples were obtained from thirty LDA and 12 healthy control cattle. Plasma from LDA cattle exhibited significantly higher free fatty acid and β-hydroxybutyrate, lower glucogenic AA, such as methionine, alanine and serine, and higher ratio of ketogenic AA among blood free AA, such as leucine and lysine. The Fischer ratio, phenylalanine and albumin were similar to control cattle. These results ...
Tópico(s): Ruminant Nutrition and Digestive Physiology
2010 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Masayuki Kohiruimaki, Tomohito Hayashi, Ken Katsuda, Keiichi Matsuda, Machiko MASUI, Ryo Abe, Seiichi Kawamura,
To clarify the relationship between cellular immune status and nutritive condition, feeding program, blood profiles, and leukocyte populations were analyzed in two dairy herds experiencing frequent mastitis. Fourteen of the 35 lactating cows in herd A, and 18 of the 50 lactating cows in herd B scored positive on the California Mastitis Test (CMT), and 3 of the 73 lactating cows were CMT positive in herd C, which was the control. All herds were evaluated during five different milking stages, and blood ...
Tópico(s): Immune Response and Inflammation
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Seiichi Higuchi, Maki IZUMITANI, Humio HOSHI, Seiichi Kawamura, Yoshio Yasuda,
Studies were made on the development of Babesia gibsoni in the midgut of the larval tick, Rhipicephalus sanguineus. Six hr after repletion, merozoites of B. gibsoni, freed from erythrocytes, were observed in the midgut contents of the tick. After that, within 24 hr, those merozoites were transformed into the ring-forms which were relatively large, 2-3 μm in diameter. Later, the ring forms developed into the spherical forms which were subelliptical in shape and 4-6 μm in diameter. Within 2-4 days, ...
Tópico(s): Insect and Pesticide Research
1999 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Ken-ichi SHIBANO, Seiichi Kawamura, Ritsuko HAKAMADA, Yozo KAWAMURA,
The serum concentration of non-essential amino acid (NEAA) was measured in ten Holstein dairy cows grouped as low production (n=5) and high production (n=5) from one month pre-partum through four months post-partum and the relationship between production and amino acid concentrations was studied. The glycine (Gly)/NEAA ratio and the glycine/alanine ratio of the high production group were significantly higher than the low production group (p<0.01). The observed decrease of the alanine (Ala)/NEAA ratio ...
Tópico(s): Genetic and phenotypic traits in livestock
2005 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Seiichi Higuchi, Sumito SIMOMURA, Hironobu YOSHIDA, Fumio Hoshi, Seiichi Kawamura, Yoshio Yasuda,
[in Japanese]
Tópico(s): Insect Pest Control Strategies
1991 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Masayuki Kohiruimaki, Hiromichi OHTSUKA, Erika TANAMI, Madoka Kitagawa, Machiko MASUI, Takaaki ANDO, Seiichi Kawamura,
The leukocyte populations of periparturient dairy cows were analyzed after administration of active egg white/Clostridium butyricum Miyairi additive. Sixty-eight Holstein milking cows were divided into 3 groups. Group A was administered active egg white product (AEWP)/Clostridium butyricum Miyairi 588 (Miyairi 588) additive (n=23). Group B was administered Miyairi 588 only (n=23), and Group C was the control group (n=22). The challenged groups were administered 100 g of AEWP + Miyairi 588, or Miyairi 588 ...
Tópico(s): Reproductive Physiology in Livestock
2008 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Daisaku WATANABE, Takashi Hirano, Yoshikazu Sugimoto, Yoshimi Ogata, Shogo Abe, Takaaki ANDO, Hiromichi OHTSUKA, Tetsuo Kunieda, Seiichi Kawamura,
Blood examinations and genotyping of Factor XI (F11) were performed in growth retardation Japanese Black cattle and their dams. Genotyping of F11 revealed that the recessive homozygous and heterozygous genotype frequencies were 5.2% and 50.0% in the Claudin-16 (CL-16) deficiency group (n=58), 0% and 14.2% in the renal dysplasia group (n=7), 0% and 26.1% in the non-CL-16 deficiency nephritis group (n=23), 8.9% and 46.7% in the hypogenesis syndrome group (n=45), 6.2% and 25.0% in the neonatal weak calf ...
Tópico(s): Barrier Structure and Function Studies
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Shoh-ichi KOMATSU, Satoru Konnai, Shigeo Fukuda, Yoshio Kiku, Tomoo YOSHINO, Masateru Koiwa, Seiichi Kawamura,
To evaluate cellular immunity in Japanese Black (JB) calves, changes in peripheral leucocytes were com-pared in two groups of calves–JB calves (JB group;n=10) and Holstein calves (Hol group; n=10). In both groups flow cytometric analysis (FCM) was used within 3 days (0 week) and 3, 6, 9, and 12 weeks after birth.Forward (FSC) and side-scatter (SSC) dot-plots were examined and used to construct monocyte-lymphocytes gates of 3 sizes (small, medium, and large). In both groups, numbers of medium (M)-ML were ...
Tópico(s): Microbial infections and disease research
2002 - Canadian Veterinary Medical Association | Journal of the Japan Veterinary Medical Association
Nobuhiro Terasaki, Atsuya OGATA, Hiromichi OHTSUKA, Keiji Tamura, Fumio Hoshi, Masateru Koiwa, Seiichi Kawamura,
Changes in subsets of peripheral blood mononuclear cells and lymphocyte blastogenesis reaction were observed after ozonated autohemoadministration (OAHA) in calves. Ten healthy calves were used in this study. After OAHA, CD8+ cells, CD14+ cells and WC1+cells increased (p<0.05). The level of lymphocyte blastogenesis stimulated by PHA decreased after OAHA. A significant increase in serum IL-6 activity was observed in OAHA calves. These results suggested that OAHA induced immunological changes in calves.
Tópico(s): Xenotransplantation and immune response
2001 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Hiromichi OHTSUKA, Narumi Fukunaga, Shigeo Fukuda, Atsushi HATSUGAYA, Tomohito Hayashi, Hidefumi Hara, Masateru Koiwa, Ryo Abe, Seiichi Kawamura,
To clarify the effect of nutritive conditions on changes in immune cells in Japanese Black (JB) calves during the growth period, leukocyte populations were analyzed in ten healthy JB calves managed in one herd. The calves were divided into two groups: five calves in Group 1 were given insufficient nutrition, and the other five calves in Group 2 received adequate nutrition. The levels of serum total cholesterol and glucose were significantly lower in Group 1 than in Group 2 at 1 month. The numbers ...
Tópico(s): Animal Disease Management and Epidemiology
2005 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Arifur Rahman, Seiichi Kawamura, Masahiro Hatsu, Md. Mozammel Hoq, Kazuhiro Takamizawa,
The zygomycete fungus Rhizomucor pusillus HHT-1, cultured on L(+)arabinose as a sole carbon source, produced extracellular alpha-L-arabinofuranosidase. The enzyme was purified by (NH4)2SO4 fractionation, gel filtration, and ion exchange chromatography. The molecular mass of this monomeric enzyme was 88 kDa. The native enzyme had a pI of 4.2 and displayed a pH optimum and stability of 4.0 and 7.0-10.0, respectively. The temperature optimum was 65 degrees C, and it was stable up to 70 degrees C. The ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2001 - Canadian Science Publishing | Canadian Journal of Microbiology
Hiromichi OHTSUKA, Masami Uematsu, Yumi Saruyama, Maiko ONO, Masayuki Kohiruimaki, Takaaki ANDO, Seiichi Kawamura,
Older cows show a high incidence of infectious diseases during the periparturient period. The periparturient infectious diseases are closely associated with the immune function of dairy cows during the pre-calving period. In order to evaluate the relationship between the immune cell population and age in the cows during the pre-calving period, we obtained blood samples from 170 dairy cows during the pre-calving period. We chose only healthy cows, which did not develop clinical diseases within 2 ...
Tópico(s): Immune Cell Function and Interaction
2009 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
M. Kubota, Tomohito Hayashi, Kenji Iwasaki, Hiromichi OHTSUKA, Masayuki Kohiruimaki, Seiichi Kawamura, Kengo Sakaguchi, Ryosuke Abe,
Quantitative PCR can be an effective method for identifying the bacteria causing mastitis. However, PCR detection is hampered by the presence of inflammatory somatic cells. To eliminate this problem, we attempted to establish methods that allow the effective separation of bacterial cells from somatic cells in mastitis milk with amino-silica. Somatic cells and Staphylococcus aureus cells have different sizes, surface structures, and overall electrical charges; therefore, their adsorption and desorption ...
Tópico(s): Enterobacteriaceae and Cronobacter Research
2007 - Elsevier BV | Journal of Dairy Science
R. Ando, Akihiko Yonezawa, Chie Watanabe, Seiichi Kawamura,
This study assessed the flexor reflex induced by intraarterial algogenic drugs in anesthetized rats. The experiments were performed on male Sprague Dawley rats weighing 290-350 g. The animals were anesthetized with urethane (1.3 g/kg i.p.) and an arterial cannula was inserted to the level of the bifurcation of the femoral artery. The magnitude of the flexor reflex was examined by recording the electromyograph from the posterior biceps femoris/semitendinous muscles. Results showed that the flexor ...
Tópico(s): Pain Management and Treatment
2004 - Thomson Reuters | Methods and Findings in Experimental and Clinical Pharmacology
Seiichi Kawamura, Hideyuki Ikematsu, H Ogimoto,
We experienced two cases of atypical pneumonia accompanied with rhabdomyolysis and diagnosed them as psittacosis based on the results of investigation of paired serum samples. Rhabdomyolysis may be associated with some kinds of viral or bacterial infections, but no report was found in the case of psittacosis as far as we searched and such a description was not found in textbooks. Heretofore, the elevation of GOT and LDH as the laboratory findings in psittacosis was considered as the reflection of ...
Tópico(s): Cardiovascular Effects of Exercise
1990 - | Kansenshogaku zasshi
Seiichi Higuchi, Kazuhiko EZURA, M Hamana, Seiichi Kawamura, Yoshio Yasuda,
Studies were made on the development of Babesia ovata in the midgut of the nymphal tick vector, Haemaphysalis longicornis. In 12 hr post-repletion, merozoites were observed outside of erythrocytes infected with B. ovata in the contents of the midgut of the tick. After that, these merozoites were transformed into ring-forms which were comparatively large ring 2-3μm in diameter. Within 48-72 hr post-repletion, ring-form protozoa developed into spherical form 4/5μm in diameter. Within 3-4 days post- ...
Tópico(s): Agricultural pest management studies
1989 - Japanese Society of Veterinary Science | The Japanese Journal of Veterinary Science
Naoyuki Itoh, M. Agri.d, Seiichi Higuchi, Seiichi Kawamura, Itoh's,
Summary The effect of diminazene aceturate on splenectomized and nonsplenectomized dogs with Babesia gibsoni (B. gibsoni) infection was investigated. In splenectomized dogs, the fissional and multiplicational stages of B. gibsoni were observed in peripheral blood films, and hemoglobinuria was frequently observed. These findings were different from previous reports 1–6 and were not changed by administration of diminazene aceturate. It is clear that the intramuscular administration of diminazene aceturate ...
Tópico(s): Animal Nutrition and Physiology
1988 - Wiley | Veterinary Clinical Pathology
Masayuki Kohiruimaki, Hiromichi OHTSUKA, Tomohito Hayashi, Kayoko Kimura, Machiko MASUI, Takaaki ANDO, Daisaku WATANABE, Seiichi Kawamura,
The purpose of this study was to clarify whether weight change rate (WCR) is useful in evaluating herd health. Five herds (A, B, C, D, and E) were examined monthly for approximately 1 year to measure both WCR and BCS. WCR was calculated using the following equation: WCR (%) = (body weight of current month-body weight of the preceding month)/body weight of current month x 100. In addition, blood profiles were performed in each herd before and after calving. The incidence of periparturient diseases was higher ...
Tópico(s): Ruminant Nutrition and Digestive Physiology
2006 - Japanese Society of Veterinary Science | Journal of Veterinary Medical Science
Susumi Hatakeyama, Mitsuhiro Kawamura, Seiichi Takano,
... RETURN TO ISSUEPREVArticleNEXTTotal Synthesis of (-)-PaeoniflorinSusumi Hatakeyama, Mitsuhiro Kawamura, and Seiichi TakanoCite this: J. Am. Chem. Soc. 1994, 116, ...
Tópico(s): Alkaloids: synthesis and pharmacology
1994 - American Chemical Society | Journal of the American Chemical Society