Naoko Sugihara, Ayato Kanda, Teruyuki Nakano, Takahiro Nakamura, Hisao Igusa, Setsuko Hara,
Since deodorization distillate, a by-product of rice bran oil production, contains squalene (ca. 8%) and phytosterols (ca. 4%) as unsaponifiable components, the concentration of those materials for their use in the cosmetics and food industries is desirable. In the present work, a novel fractionation method of concentrating squalene and phytosterols from deodorization distillate or the unsaponifiable components of the deodorization distillate without oxidative deterioration was examined. Supercritical ...
Tópico(s): Chemical and Physical Properties in Aqueous Solutions
2010 - Japan Oil Chemists' Society | Journal of Oleo Science
Shigesaburo Ogawa, Ayano Endo, Naoki Kitahara, Takayuki Yamagishi, Satoka Aoyagi, Setsuko Hara,
Solvent-free synthesis encourages the design of processes and products that reduce the use and generation of hazardous chemicals. Given the importance of developing greener methodologies, we sought to determine the factors influencing the reaction temperature required for solvent-free, enzymatic synthesis of sugar esters such as trehalose (TRE) esters, using Novozyme 435 as the enzyme catalyst. The use of lauric acid (La) and ethyl laurate (LaEt) as acyl donors did not affect the activation temperature ...
Tópico(s): Enzyme Production and Characterization
2019 - Elsevier BV | Carbohydrate Research
Ayano Yoshie, Ayato Kanda, Takahiro Nakamura, Hisao Igusa, Setsuko Hara,
Although there are various determination methods for γ -oryzanol contained in rice bran oil by absorptiometry, normal-phase HPLC, and reversed-phase HPLC, their accuracies and the correlations among them have not been revealed yet. Chloroform-containing mixed solvents are widely used as mobile phases in some HPLC methods, but researchers have been apprehensive about its use in terms of safety for the human body and the environment. In the present study, a simple and accurate determination method ...
Tópico(s): Cholesterol and Lipid Metabolism
2009 - Japan Oil Chemists' Society | Journal of Oleo Science
Atsushi Utsugi, Ayato Kanda, Setsuko Hara,
The Lipase-catalyzed transesterification method is utilized as a safe and effective method for preparing various structured oils. As each lipase shows different fatty acid specificity, it is important to select an appropriate lipase according to fatty acid species incorporated into oils. In the present study fatty acid specificities of lipases obtained from different origins were evaluated by transacylation between oils and various fatty acids. Of 12 kinds of lipases used, 5 lipases have 1,3-regio ...
Tópico(s): Lipid metabolism and biosynthesis
2009 - Japan Oil Chemists' Society | Journal of Oleo Science
Ryo Fukasawa, Ayato Kanda, Setsuko Hara,
Powdered rooibos tea extract (RTE), which is rich in polyphenols, is made from rooibos tea by freeze-drying. "Rooibos" is Afrikaans for "red bush," and the scientific name is "Aspalathus linearis." It is a broom-like member of the legume family of plants and is used to make an herbal tea which has been popular in South Africa for generations and is now consumed in many countries. In the present work, the anti-oxidative effect of RTE on oils and fats in autoxidation or thermal oxidation was studied, ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2009 - Japan Oil Chemists' Society | Journal of Oleo Science
Setsuko Hara, Eri Ogawa, Yoichiro Totani,
A simple and accurate method for determining polar compound (PC) content in heat-deteriorated oils was developed, since universal standards are necessary for assessing thermal rancidity in frying oil quality control. At present, the means used for standards differ according to countries. For instance, in Japan the upper limit of acid value (AV) is 2.5 and carbonyl value (CV) is 50, in the United States free fatty acid (FFA) content is less than 2.0%, and in EU countries PC content is below about 25% ...
Tópico(s): Fermentation and Sensory Analysis
2006 - Japan Oil Chemists' Society | Journal of Oleo Science
Takeshi Segawa, Masazumi Kamata, Setsuko Hara, Yoichiro Totani,
The antioxidative and synergistic effects of nitrogen-containing phospholipids such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PMME), phosphatidyl-N, N-dimethylethanolamine (PDME), and phosphatidylserine (PS) were investigated using fish oil as the polyunsaturated substrate during autoxidation at 30°C in the dark. The phospholipids had remarkable synergistic effect on mixed tocopherol (Toc).Based on the increase in time for each Toc isomer to disappear ...
Tópico(s): Antioxidant Activity and Oxidative Stress
1995 - Japan Oil Chemists' Society | Yukagaku
Yoichiro Totani, Setsuko Hara,
Abstract Transesterifications were investigated to determine a means for preparing polyunsaturated phospholipids simply from soy phospholipid, sardine oil, and two kinds of microbial lipases originating from Candida cylindracea and Rhizopus delemar . The optimum reaction conditions for Candida cylindracea lipase were: 4 g of sardine oil, 10 mL of water, 0.7 g of lipase, 10 mL of hexane, 48 hr of reaction time at 37°C for 3 g of soy phospholipid, for which the transesterification ratio reached approximately ...
Tópico(s): Fatty Acid Research and Health
1991 - Wiley | Journal of the American Oil Chemists Society
Setsuko Hara, Yoichiro Totani,
Abstract A modified method for peroxide value (POV) determination of lipids was developed through the application of potentiometry to conventional POV tests such as the official method of the Japan Oil Chemists’ Society (JOCS). The new method permits a simple and reliable determination of low hydroperoxide levels in the initial stages of lipid autoxidation when only very small amounts of sample are available, even when those levels are measured on less than 10 mg of lipid. Using the present method, ...
Tópico(s): Analytical Chemistry and Chromatography
1988 - Wiley | Journal of the American Oil Chemists Society
Implicational Spectatorship:Hara Setsuko and the Queer Joke Yuka Kanno (bio) What does it mean to have evidence of someone's sexual ... late 1940s through the 1960s, the name of Hara Setsuko is always evoked with a certain nostalgia. For ... nostalgia. Like many others, I was struck by Hara Setsuko, not so much by her legendary beauty as ...
Tópico(s): Asian Culture and Media Studies
2011 - University of Minnesota Press | Mechademia Second Arc
Kousuke Kotani, Y. Yamamoto, Setsuko Hara,
Human milk fat substitutes (HMFSs), rich in palmitic acid (P) at the sn-2 position of triacylglycerol (TAG), were prepared from lard via Novozym435®- and Lipozyme RM-IM®-mediated two-step reactions. First, 2-palmitoyl monoacylglycerol (2-P-MAG, 90% purity) was prepared via Novozym435®mediated ethanolysis of lard. Then, 2-P-MAG, oleic acid (O), linoleic acid (L), and lard were dissolved in hexane and subjected to a Lipozyme RM-IM®-mediated reaction for HMFS preparation. The effect of the amount of 2-P- ...
Tópico(s): Protein Hydrolysis and Bioactive Peptides
2015 - Japan Oil Chemists' Society | Journal of Oleo Science
Y. Yamamoto, Yuka Imori, Setsuko Hara,
The location of unsaturated fatty acid in triacylglycerols (TAGs) affects their oxidative stability. Herein, we studied the effect of location of conjugated linolenic acids (CLNs) in TAGs on their oxidative stability. First, the TAGs containing CLNs at sn-1(3) and sn-2 positions, were enzymatically prepared via Lipozyme RM-IM (immobilized Rhizomucor miehei lipase)-mediated acidolysis- and contained ca. 60% of CLNs at sn-1(3) or sn-2 position. The auto-oxidation test at 30°C in the dark showed oxidative ...
Tópico(s): Advanced Glycation End Products research
2013 - Japan Oil Chemists' Society | Journal of Oleo Science
Masaru Takeuchi, Setsuko Hara, Yoichiro Totani, Hidehiko Hibino, Yukihisa Tanaka,
高度不飽和リン脂質に富む筋子油の自動酸化挙動をオキアミ油とサケ油の自動酸化挙動と比較した。筋子油とオキアミ油はカロテノイドの一種であるアスタキサンチンとリン脂質を含むため, 30℃の自動酸化に対してサケ油よりもはるかに安定であった。次に, 筋子油とそのトリアシルグリセリン及びリン脂質区分の酸化安定性を同条件下に評価した。その結果, 筋子油中のヒドロペルオキシドの蓄積は筋子油に含まれるアスタキサンチンとトコフェロールの酸化防止効果により, トリアシルグリセリン区分やリン脂質区分中よりもかなり抑制された。それ故, 高度不飽和脂肪酸に富む脂質の自動酸化はアスタキサンチン, トコフェロール及びリン脂質が共存することによって抑制され, リン脂質はトコフェロールに対する相乗剤として作用した。
Tópico(s): Inorganic and Organometallic Chemistry
1997 - | Journal of Japan Oil Chemists Society
Takeshi Segawa, Setsuko Hara, Yoichiro Totani,
Tópico(s): Various Chemistry Research Topics
1994 - Japan Oil Chemists' Society | Yukagaku
Takeshi Segawa, Setsuko Hara, Yoichiro Totani,
The antioxidative and synergistic effects of glycerophospholipids (PL) such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidil-inositol (PI) were investigated using fish oil as a substrate at 30°C in the dark.PC was noted to express antioxidative effect, PE strong synergistic effect, PG proxidative effect and PI to have no effect at all. During autoxidation, mixed tocopherols added to the substrate were consumed in the order, α-, γ-δ-isomers, with ...
Tópico(s): Edible Oils Quality and Analysis
1994 - Japan Oil Chemists' Society | Yukagaku
... of the most popular actresses of the time, Hara Setsuko, Takamine Hideko, and Sugimura Haruko, were identified strongly ... of the top stars of this period. Both Hara Setsuko and Mifune Toshiro had slightly Caucasian features, suggesting ...
Tópico(s): Hong Kong and Taiwan Politics
2003 - | Cineaction!
Shigesaburo Ogawa, Mai Shinkawa, Ryuji Hirase, Taro Tsubomura, Katsuya Iuchi, Setsuko Hara,
Development of a novel antioxidant-delivery vehicle exerting biosafety has been attracting a great deal of interest. In this study, a vehicle comprising a natural composite consisting of vitamin E (α-tocopherol; Toc) and cyclodextrin (CD) additives was developed, directed toward aqua-related biological applications. Not only β-CD, but also γ-CD, tended to form a water-insoluble aggregate with Toc in aqueous media. The aggregated vehicle, in particular the γ-CD-added system, showed a remarkable sustained ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2021 - Multidisciplinary Digital Publishing Institute | Antioxidants
S Ishikawa, Atsushi Maruyama, Y. Yamamoto, Setsuko Hara,
While some isothiocyanate (ITCs) are attractive targets for the agricultural and pharmaceutical industries, the presence of goitrin and ITCs has hampered the widespread utilization of rapeseed meal. ITCs are the products of the myrosinase-mediated hydrolysis of glucosinolate (GSLs). As such, a study was conducted in order to gain a better understanding into the identity of the GSLs contained in rapeseed meal. Extraction of the GSLs was carried out with 20% ethanol, affording 3.0% GSL content. The ...
Tópico(s): Insect Pest Control Strategies
2014 - Japan Oil Chemists' Society | Journal of Oleo Science
Setsuko Hara, Hiroyuki HASUO, Makoto Nakasato, Yuzo HIGAKI, Yoichiro Totani,
Modified glycerophospholipids with expected novel physiological functions were prepared by introducing different acyl groups into soybean phospholipids. The method was based on the enzymatic transesterification of phospholipids with monoacylglycerol (MG) or diacylglycerol (DG). The transacylation ratios reached 46-59% and nearly quantitative recovery of phospholipids was obtained in the case of transacylation of phospholipids with MGs having C12 to C18 saturated and monounsaturated acyl groups. With ...
Tópico(s): Metabolism and Genetic Disorders
2002 - Japan Oil Chemists' Society | Journal of Oleo Science
Shigesaburo Ogawa, Koji Honda, Taro Tsubomura, Kiichiro Totani, Isao Takahashi, Setsuko Hara,
The understanding of the basic physicochemical properties of trehalose lipid is indispensable to extending their availability. In this study, the hydrate crystal (Cr), the liquid crystalline (LC) phase and the glassy state formations of 6-O-acyl trehalose fatty acid monoester (TREn) were examined under in a desiccated system. TREn (n = 10, 12, 14, 16) formed monohydrate Cr and showed the hydrate Cr-glassy fluid lamellar LC (Lα) phase transition via dehydration in the heating process. Here, Lα phase for ...
Tópico(s): Material Dynamics and Properties
2018 - Elsevier BV | Chemistry and Physics of Lipids
Setsuko Hara, Norio OKADA, Hidehiko Hibino, Yoichiro Totani,
The autoxidation of certain polyunsaturated triglycerides (PUFA-TG) such as fish oils was inhibited effectively by the addition of 5 % soybean phospholipids (PL) to PUFA-TG used as a substrate. The antioxidative effect of PL became greater with increase in phosphatidylcholine (PC) content in PL. The antioxidative effect of mixed tocopherol (Toc) also increased synergistically by adding 0.1 % Toc and 1 % PL to the substrate.The effects of each phospholipid class such as PC, phosphatidylethanolamine (PE) and ...
Tópico(s): Pesticide Exposure and Toxicity
1992 - Japan Oil Chemists' Society | Yukagaku
Y. Yamamoto, Eri Mizuta, Mizue Ito, Masataka Harata, Shigeru Hiramoto, Setsuko Hara,
To utilize n-3 polyunsaturated fatty acid (PUFA) for a wide range of applications, we prepared phospholipids (PLs) containing PUFAs as constituent fatty acids (PUFA-PLs) via commercially available lipase OF-mediated transacylation with PL from soy (Soy-PL) and ethyl ester of PUFA (PUFA-Et). In a preliminary study to evaluate PUFA-incorporation (wt%) on phosphatidylcholine (PC), we observed that dehydration of Soy-PL is critical. PUFA-incorporation in PLs increased with acyl ratio and time. Finally, maximum ...
Tópico(s): Enzyme Catalysis and Immobilization
2014 - Japan Oil Chemists' Society | Journal of Oleo Science
Tópico(s): Sarcoma Diagnosis and Treatment
1999 - Soc for Publication of Acta Dermato-Venereologica | Acta Dermato Venereologica
Shigesaburo Ogawa, Ryuji Hirase, Tomoyuki Ohishi, Setsuko Hara, Isao Takahashi,
Abstract Ascorbic acid 6‐palmitate (AsP) is an industrially utilized antioxidant and amphiphilic lipid, which forms a liquid crystalline (LC) phase. Herein, we describe the basic thermal behavior of AsP under anhydrous condition. Grazing‐incidence wide‐angle X‐ray‐diffraction (GI‐WAXD) measurements using a two‐dimensional detector (2D‐GI‐WAXD) and perpendicularly aligned AsP films, together with thermal analysis, showed that the AsP forms only a lamellar LC phase above its melting point ( T m ); however, ...
Tópico(s): Bee Products Chemical Analysis
2020 - Wiley | ChemistrySelect
Shinsuke Dote, Y. Yamamoto, Setsuko Hara,
Two kinds of oils, pure perilla oil and a blend of perilla oil with palm oil, and their enzymatically interesterified oils having the same fatty acid compositions but with different compositions of triacylglycerol (TAG) species, were studied. In particular, the effects of TAG molecular species on the oxidation resistance of oils containing α-linolenic acid (Ln) were investigated. The content of TAG binding to three Ln molecules (3Ln-TAG) was found to be different between perilla oil (38.7%) and the ...
Tópico(s): Food Chemistry and Fat Analysis
2016 - Japan Oil Chemists' Society | Journal of Oleo Science
Y. Yamamoto, Shinichiro Hiyama, Yoshihiko Takase, Akio Kadowaki, Setsuko Hara,
The effects of antioxidants on the oxidative deterioration of emulsified oils and fats differ depending on the oxidation conditions, oils and fats used, and type of emulsifier. In this study, milk fat was emulsified to obtain water-oil (O/W) emulsion using Tween20 as emulsifier. The antioxidative effects of several antioxidants with various lipophilic properties, such as δ-tocopherol (Toc), epigallocatechin gallate (EGCg), quercetin (Qu), green tea extract (GTE), and rooibos tea extract (RTE) were investigated, ...
Tópico(s): Protein Hydrolysis and Bioactive Peptides
2014 - Japan Oil Chemists' Society | Journal of Oleo Science
Yohko Kuge, Ayato Kanda, Setsuko Hara,
Although conjugated oils are paid much attentions to their interesting physiological properties such as anticancer, anti-arteriosclerosis, anti-hypertension activities, loss in body fat etc, there is few information on their oxidation behavior. In the present work, their oxidation behavior and oxidation prevention method were evaluated to utilize as functional foods or drugs. As results, an oxidation behavior of conjugated oils was different from that of corresponding non-conjugated oils, and conjugated ...
Tópico(s): Edible Oils Quality and Analysis
2009 - Japan Oil Chemists' Society | Journal of Oleo Science
Setsuko Hara, Yoshiyuki Kohno, Minoru Aoyama, Shuichi Abe, Tsugio ISOBE, Motoko KAKUTA, Kenichi KURIYAMA, Kazuhiro Nakamura, Yoshiaki Hirata, Yuko Miyake, Youichi Watanabe,
A novel method using isooctane was established for determining the peroxide value (PV), widely used as basis for assessing the rancidity of oil and fat. This was done through the collaboration of 10 research organizations by potentiometry using an automatic titrator equipped with a platinum complex electrode.The potentiometric PV method gave a 10% higher value than by the official method of the Japan Oil Chemists' Society using isooctane (JOCS-I method) for samples with PV from 0.5 to 100 meq/kg. ...
Tópico(s): Electrochemical sensors and biosensors
1997 - | Journal of Japan Oil Chemists Society
Takao Ishizuka, Yutaka Tomoda, S Kaseki, Setsuko Goto, Takako Hara, Tatsuya Kobayashi,
In an attempt to improve the diagnosis and treatment of intracranial metastases of choriocarcinoma, the authors carried out a clinicopathologic investigation of 36 patients with choriocarcinoma metastasized in the brain. Analysis on the autopsy findings of 30 cases with documented intracranial metastases of choriocarcinoma proved neurosurgical resectability in most cases. After initiation of the clinical use of dactinomycin in 1965, 5 of 10 patients in the surgically treated group, and 1 of 17 in ...
Tópico(s): Brain Metastases and Treatment
1983 - Wiley | Cancer
Shin Ogasawara, Shigesaburo Ogawa, Y. Yamamoto, Setsuko Hara,
The development of human milk fat substitutes (HMFSs), rich in palmitic acid (16:0) at the sn-2 position of triacylglycerol (TAG) and rich in unsaturated fatty acids (FAs) (oleic acid, 18:1 and linoleic acid, 18:2) at the sn-1(3) positions, has gained popularity. In this study, HMFSs containing polyunsaturated fatty acids (PUFAs) predominantly at the sn-2 position were prepared, and their oxidation stabilities were compared. First, a non-PUFA-containing HMFS (NP-HMFS) was produced by enzymatic reactions ...
Tópico(s): Advanced Glycation End Products research
2020 - Japan Oil Chemists' Society | Journal of Oleo Science