Shahar Frenkel, Jacob Pe’er, Ron Kaufman, Bella Maly, Zohar Habot‐Wilner,
To evaluate the efficacy of the different diagnostic tests for vitreoretinal lymphoma (VRL).A cohort of 150 patients with a presumed diagnosis of VRL. Vitrectomy samples were analysed for cytology, monoclonality [polymerase chain reaction (PCR)] and cytokine levels, and anterior chamber taps were analysed for cytokine levels. Vitreoretinal lymphoma (VRL) was diagnosed after confirming the clinical suspicion with vitreal or brain cytology.Vitreoretinal lymphoma (VRL) was diagnosed in 78 patients. Vitreal ...
Tópico(s): Viral-associated cancers and disorders
2020 - Wiley | Acta Ophthalmologica
Zohar Habot‐Wilner, Shahar Frenkel, Jacob Pe’er,
Summary Vitreo‐retinal lymphoma (VRL) is the most common intraocular lymphoma and is highly associated with central nervous system (CNS) lymphoma (CNSL), both posing a therapeutic challenge. We investigated patients’ characteristics, efficacy and safety of intravitreal methotrexate (MTX) injections and their outcomes over 20 years. The records of 129 patients diagnosed between 1997 and 2018 were retrospectively reviewed. Lymphoma involved both the CNS and vitreo‐retina (49%), solely the CNS (37%) or solely ...
Tópico(s): Vascular Tumors and Angiosarcomas
2021 - Wiley | British Journal of Haematology
Produção Nacional
Revisado por pares
Denise Carbonell, Sarakshi Mahajan, Soon‐Phaik Chee, Bianka Sobolewska, Rupesh Agrawal, Tanja Bülow, Vishali Gupta, Nicholas P. Jones, Massimo Accorinti, Mamta Agarwal, Tracy T. Batchelor, Jyotirmay Biswas, Luca Cimino, Ninette H. tenDam-van Loon, Alejandra de‐la‐Torre, Shahar Frenkel, Jacob Pe’er, Michal Kramer, Elisabetta Miserocchi, Manabu Mochizuki, Thomas Neß, James T. Rosenbaum, H. Nida Sen, Michael Simion, H. Sitter, Daniel V. Vasconcelos-Santos, Zohar Habot‐Wilner, Sarah E. Coupland, José S. Pulido, Justine R. Smith, Jennifer E. Thorne, Manfred Zierhut,
Purpose To provide recommendations for diagnosis of vitreoretinal lymphoma (VRL).Methods Literature was reviewed for reports supporting the diagnosis of VRL. A questionnaire (Delphi 1 round) was distributed to 28 participants. In the second round (Delphi 2), items of the questionnaire not reaching consensus (75% agreement) were discussed to finalize the recommendations.Results Presenting symptoms include floaters and painless loss of vision, vitreous cells organized into sheets or clumps. Retinal lesions ...
Tópico(s): Viral-associated cancers and disorders
2021 - Taylor & Francis | Ocular Immunology and Inflammation
Rana’a T. Al‐Jamal, Nathalie Cassoux, Laurence Desjardins, Bertil Damato, Lazaros Konstantinidis, Sarah E. Coupland, Heinrich Heimann, Aleksandra Petrović, Léonidas Zografos, Ann Schalenbourg, Juan P. Velazquez-Martin, Hatem Krema, Anna Bogdali, Anna Markiewicz, Bożena Romanowska‐Dixon, Claudia H.D. Metz, Eva Biewald, Norbert Bornfeld, Hayyam Kıratlı, Inge H. G. Bronkhorst, Martine J. Jager, Marina Marinkovic, Maria Filì, Stefan Seregard, Shahar Frenkel, Jacob Pe’er, Sachin M. Salvi, I G Rennie, Iwona Rospond‐Kubiak, Jarosław Kocięcki, Jens Folke Kiilgaard, Steffen Heegaard, Victoria M.L. Cohen, Mandeep S. Sagoo, A.G. Amiryan, S. V. Saakyan, Nils Eide, Jørgen Krohn, Edoardo Midena, Raffaele Parrozzani, J.D. Grange, Emine Kılıç, Maria Antonietta Blasi, María A. Saornil, Tero Kivelä,
Tópico(s): Glaucoma and retinal disorders
2016 - Elsevier BV | Ophthalmology
Michal Sagi, Avishag Frenkel, Avital Eilat, Naomi Weinberg, Shahar Frenkel, Jacob Pe’er, Dvorah Abeliovich, Israela Lerer,
Tópico(s): Hedgehog Signaling Pathway Studies
2015 - Springer Science+Business Media | Familial Cancer
Olga Dratviman‐Storobinsky, Yoram Cohen, Shahar Frenkel, Jacob Pe’er, Nitza Goldenberg‐Cohen,
Purpose.: Somatic mutations in codon 209 of the GNAQ gene are the first initiating events to be identified in uveal melanoma. The purpose of this study was to search for GNAQ209 mutations in conjunctival melanocytic lesions. Methods.: Forty archival samples of conjunctival melanocytic lesions (conjunctival nevi, primary acquired melanosis, and conjunctival melanoma), 27 samples of uveal melanoma, and 11 samples of uveal melanoma metastases to the liver (3 of which matched primary uveal melanoma samples)— ...
Tópico(s): Cancer and Skin Lesions
2010 - Cadmus Press | Investigative Ophthalmology & Visual Science
Panagiota S. Filippou, Annie Ren, Dimitrios Korbakis, Lampros Dimitrakopoulos, Antoninus Soosaipillai, Vivian Barak, Shahar Frenkel, Jacob Pe’er, Michal Lotem, Sharon Merims, Rafael Molina, Ivan M. Blasutig, Dimitrios P. Bogdanos, Eleftherios P. Diamandis,
Mucin 13 (MUC13) is a cell surface glycoprotein aberrantly expressed in a variety of epithelial carcinomas. Thus far, the role of MUC13 in various diseases remains elusive. To the best of our knowledge, this is the first study to examine the potential of MUC13 as a serum biomarker in a variety of carcinomas and other conditions.We developed a recombinant MUC13 protein, mouse monoclonal antibodies and enzyme immunoassay (ELISA) for MUC13. We used this assay to measure MUC13 levels in the supernatants ...
Tópico(s): Immunotherapy and Immune Responses
2018 - De Gruyter | Clinical Chemistry and Laboratory Medicine (CCLM)
Yoram Cohen, Efrat Merhavi-Shoham, Bat-Chen R. Avraham-Lubin, Michael Savetsky, Shahar Frenkel, Jacob Pe’er, Nitza Goldenberg‐Cohen,
Purpose.: Many malignancies are known to be associated with abnormal activation of the PI3K-AKT pathway. Recently, a somatic mutation in the AKT1 gene (E17K) was identified in a small proportion of human tumors. This mutation activated AKT1 by means of abnormal membrane recruitment and stimulated downstream signaling. This study was designed to analyze AKT1 mutations in retinoblastoma and gain insights into the role PI3K-AKT pathway plays in the development of this tumor. Methods.: Twenty-four samples ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2009 - Cadmus Press | Investigative Ophthalmology & Visual Science
Nadia Kaiserman, Igor Kaiserman, Karen Hendler, Shahar Frenkel, J. Pe’er,
Aims: Ruthenium-106 brachytherapy is an effective method for treating small to medium uveal melanomas. The purpose of this study was to examine its effectiveness and safety in the management of thick posterior uveal melanoma (apical height ⩾8.0 mm) and to compare it with enucleation. Methods: 126 consecutive patients with thick uveal melanoma were included. 63 patients treated with Ruthenium-106 brachytherapy were compared with 63 patients treated with enucleation. The main outcome measures were visual ...
Tópico(s): Ocular Infections and Treatments
2009 - BMJ | British Journal of Ophthalmology
Yoram Cohen, Efrat Merhavi-Shoham, Revital B. Avraham, Shahar Frenkel, Jacob Pe’er, Nitza Goldenberg‐Cohen,
Tópico(s): Ocular Oncology and Treatments
2008 - Elsevier BV | Experimental Eye Research
Ofira Zloto, Jacob Pe’er, Shahar Frenkel,
Purpose.: We examined the clinical differences in manifestation and prognosis of uveal melanoma (UM) between men and women. Methods.: We evaluated 723 UM patients (325 males) who were treated between 1988 and 2010 at a national referral center. Men and women were compared regarding differences in annual distribution, age at diagnosis, size and intraocular location of the tumor, symptoms leading to diagnosis, recurrence, development of metastases, and mortality. Statistical analysis included ANOVA, ...
Tópico(s): Nonmelanoma Skin Cancer Studies
2012 - Cadmus Press | Investigative Ophthalmology & Visual Science
Naomi Weintraub, Nurit Reshef, Jacob Pe’er, Shahar Frenkel, Ido Rot, Naomi Shoshani, Michael Weintraub,
Abstract Background Monocular vision has been found to have a negative effect on children's motion processing and motor functions. Yet, knowledge of motor function of survivors of retinoblastoma (RB) with monocular vision (due to enucleation, for example) is limited. This study examined motor function and its relationship to visual‐related and health‐related quality of life (HRQOL) in survivors of RB with monocular vision. Procedure Parents of 27 survivors of RB, who underwent an enucleation of one ...
Tópico(s): Cytomegalovirus and herpesvirus research
2019 - Wiley | Pediatric Blood & Cancer
Shahar Frenkel, Ofira Zloto, Jacob Pe’er, Vivian Barak,
High expression levels of insulin-like growth factor-1 (IGF-1) receptor were associated with metastatic uveal melanoma (UM). The purpose of this study was to examine the potential of serum IGF-1 in early detection of liver metastasis.IGF-1 serum levels were analyzed using enzyme-linked immunosorbent assay for 118 subjects in three different groups: 55 disease-free (DF) UM patients who did not develop metastasis within 10 years of diagnosis; 22 metastatic patients; and 41 healthy subjects. Matched pairs ...
Tópico(s): Cancer and Skin Lesions
2012 - Cadmus Press | Investigative Ophthalmology & Visual Science
Efrat Merhavi, Yoram Cohen, Bat Chen R. Avraham, Shahar Frenkel, Itay Chowers, Jacob Pe’er, Nitza Goldenberg‐Cohen,
Aberrant promoter hypermethylation of CpG islands is thought to play an important role in the inactivation of tumor-suppressor genes (TSGs) in cancer. Studies of cutaneous melanoma have reported a high methylation rate for MGMT, DAPK, RAR-b2, and RASSF1A. In colon cancer, SOCS-1, IGF-2, RUNX3, NEUROG1, and CACNA1G are commonly inactivated. The concomitant methylation of at least three of these genes may represent a distinct trait, the CpG island methylator phenotype (CIMP). The purpose of the present ...
Tópico(s): Hedgehog Signaling Pathway Studies
2007 - Cadmus Press | Investigative Ophthalmology & Visual Science
Jacob Pe’er, Jacob M. Rowe, Shahar Frenkel, Eldad J. Dann,
... Hebrew University Medical Center in Jerusalem, Israel. Jacob Pe'er*, Jacob M. Rowe , Shahar Frenkel*, Eldad J. Dann , * Department of Ophthalmology, Hadassah- ...
Tópico(s): Viral-associated cancers and disorders
2010 - Wiley | American Journal of Hematology
Lauren A. Dalvin, José S. Pulido, Carol L. Shields, Alessandro Marchese, Elisabetta Miserocchi, Shahar Frenkel, Jacob Pe’er,
To investigate the risk of developing central nervous system (CNS) lymphoma in patients with vitreoretinal lymphoma (VRL) presenting with unilateral versus (vs.) bilateral ocular involvement.
Tópico(s): Viral-associated cancers and disorders
2022 - Springer Nature | Eye
Our case represents a unique occurrence of pigmented choroidal tumor that clinically appeared as choroidal melanoma and was treated accordingly. At the same time, while evaluating the patient for systemic metastases of uveal melanoma, she was diagnosed as having lung carcinoma. Events that led to the enucleation of the eye enabled a histological diagnosis: pulmonary adenocarcinoma. In our case, it happened that a very rare pigmented choroidal metastatic tumor was the presenting sign of a pulmonary ...
Tópico(s): Brain Metastases and Treatment
2012 - Karger Publishers | Case Reports in Ophthalmology
Jaime Levy, Shahar Frenkel, Mario Baras, Miriam Y. Neufeld, J. Pe’er,
Aim To evaluate the histopathologic factors statistically associated with the presence of calcification in eyes with retinoblastoma. Methods Retrospective, consecutive and observational case series. Three hundred and two enucleated eyes with retinoblastoma examined between the years 1960 and 2008. Five representative histopathologic slides of the pupil–optic nerve section and three cross optic nerve sections were retrospectively reviewed. The presence and degree of calcification as well as other histopathologic ...
Tópico(s): Soft tissue tumor case studies
2011 - BMJ | British Journal of Ophthalmology
Dudi Shneor, Robert Folberg, J. Pe’er, Alik Honigman, Shahar Frenkel,
The fast proliferation of tumor cells develops faster than the vasculature, resulting, in most malignant tumors, in generation of hypoxic regions. Hypoxia renders solid tumors resistant to radiation and chemotherapeutics while providing opportunities for tumor-selective therapies targeting tumor hypoxia. Here we exploit two properties of tumors: propagation of tumor cells and ongoing generation of hypoxic regions to construct a system that preferentially leads to the death of tumor cells and thus ...
Tópico(s): RNA modifications and cancer
2016 - Springer Nature | Cancer Gene Therapy
Vivian Barak, Jacob Pe’er, Inna Kalickman, Shahar Frenkel,
High levels of serum VEGF have been reported in many types of cancers, especially in the metastatic stage. The aim of this study was to examine the potential of VEGF serum level as a tumor marker for metastases in uveal melanoma (UM) patients.Levels of serum VEGF were analyzed by ELISA for 23 UM patients (none of whom developed metastases within 5 years from diagnosis) at the time of diagnosis, soon after treatment, and 3 years later, and compared with serum VEGF levels of 39 metastatic patients, ...
Tópico(s): Glaucoma and retinal disorders
2011 - Taylor & Francis | Current Eye Research
Robert Folberg, Shrihari S. Kadkol, Shahar Frenkel, Klara Valyi‐Nagy, Martine J. Jager, Jacob Pe’er, Andrew J. Maniotis,
Authentication of cell lines in biomedical research has been elevated to a very high priority. From a review of the literature, Lacroix1 reviewed the issue of cross-contamination of cell lines including the well known contamination of cell lines with HeLa cells,2 and the mis-identification of the ECV304 cell line as “immortalized endothelial cells” when these cells in fact originated from T24 bladder carcinoma cells.3 Lacroix 1 estimated that between 18 and 36% of cell lines have been misclassified. ...
Tópico(s): Barrier Structure and Function Studies
2008 - Cadmus Press | Investigative Ophthalmology & Visual Science
Gala Beykin, Jacob Pe’er, Gail Amir, Shahar Frenkel,
Objective To report clinical, histopathological and molecular features of 'salmon patch'-like conjunctival lesions in paediatric and adolescent patients, and discuss management of these patients and outcome. Methods Patients who presented between 2000 and 2011 with a conjunctival 'salmon-patch'-like lesion in the plical area, were identified by chart review. Clinical parameters, demographic characteristics and details of ophthalmic imaging were collected, and the effect of treatment examined. Results Eleven ...
Tópico(s): Cancer and Skin Lesions
2013 - BMJ | British Journal of Ophthalmology
Gala Beykin, Jacob Pe’er, Yitzchak Hemo, Shahar Frenkel, Itay Chowers,
Aims Retinal detachment can develop following brachytherapy for uveal melanoma. This complication may result in substantial visual loss and poses a significant therapeutic dilemma due to the required surgical intervention for correction of the detachment. We report the incidence of retinal detachment in eyes treated with brachytherapy for posterior uveal melanoma and the outcome of pars plana vitrectomy in those eyes. Methods Patients who developed tractional or combined tractional–rhegmatogenous retinal ...
Tópico(s): Corneal Surgery and Treatments
2013 - BMJ | British Journal of Ophthalmology
Iris melanomas constitute between 3% and 10% of all malignant melanomas of the uvea and, unlike posterior uveal melanoma, have a low rate of metastasis.1 The most common way of treating circumscribed iris melanoma is by partial, usually sector, iridectomy,2 with iris reconstruction where possible. When the anterior chamber angle or ciliary body is involved, iridotrabeculectomy or iridocyclectomy should be performed. Complications of iridectomy include hyphema, cataract, wound dehiscence, episcleral ...
Tópico(s): Ocular Infections and Treatments
2011 - BMJ | British Journal of Ophthalmology
Shahar Frenkel, Karen Hendler, T. Siegal, Edna Shalom, J. Pe’er,
To describe our experience in treating vitreoretinal involvement of primary central nervous system lymphoma, by intravitreal injections of methotrexate (MTX).Patients with suspected intraocular lymphoma underwent a diagnostic vitrectomy. Samples were sent for cytology, genetic evaluation and for interleukin level measurements. Treatment protocol included injection of 400 microg/0.1 ml MTX intravitreally twice weekly for 4 weeks, once weekly for 8 weeks, and then once monthly for 9 months, for a total ...
Tópico(s): Viral-associated cancers and disorders
2008 - BMJ | British Journal of Ophthalmology
Fred K. Chen, Shahar Frenkel, T. Siegal, Edna Shalom, Itay Chowers, J. Pe’er,
To determine the incidence and characteristics of maculopathy associated with blood-brain barrier disruption (BBBD) which is used in treating primary central nervous system lymphoma (PCNSL).Files of all 56 patients with PCNSL, with or without intraocular lymphoma (IOL), treated at Hadassah University Hospital during the years 1997-2007 were reviewed. Data on 46 patients for whom we had documentation of ocular examination were studied. Those who were alive at the time of the data collection were invited ...
Tópico(s): Brain Metastases and Treatment
2008 - BMJ | British Journal of Ophthalmology
Shahar Frenkel, Ofira Zloto, Michal Sagi, Avishag Fraenkel, Jacob Pe’er,
Tópico(s): Head and Neck Surgical Oncology
2016 - Elsevier BV | Experimental Eye Research
Shahar Frenkel, Gal Goshen, Lu Leach, Jacob Pe’er, Michael Mimouni, Eytan Z. Blumenthal,
The purpose of this study was to describe the distribution of Muller cell within the peripapillary retinal nerve fiber layer (RNFL) in human eyes. Eleven unpaired normal postmortem eyes were recruited into this study. Each eye was sectioned using the "umbrella technique" to obtain a concentric peripapillary ring centered on the optic disc, with a diameter of 3.0 mm. Immunohistochemistry with anti- CRALBP stained Muller cell within each ring. The RNFL thickness measurements around the peripapillary ...
Tópico(s): Retinal Diseases and Treatments
2017 - Elsevier BV | Experimental Eye Research
Yossi Mandel, Shlomi Laufer, Michael Belkin, Boris Rubinsky, Jacob Pe’er, Shahar Frenkel,
Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is characterized by high rates of metastatic disease. Although brachytherapy is the most common globe-sparing treatment option for small- and medium-sized tumors, the treatment is associated with severe adverse reactions and does not lead to increased survival rates as compared to enucleation. The use of irreversible electroporation (IRE) for tumor ablation has potential advantages in the treatment of tumors in complex ...
Tópico(s): Pulsed Power Technology Applications
2013 - Public Library of Science | PLoS ONE
J. Pe’er, E. Z Blumenthal, Shahar Frenkel,
To obtain iris biopsy samples of sufficient quality and quantity for histopathological analysis using a novel punch biopsy technique.Two patients underwent iris tumour biopsy at an ocular oncology service. A trabeculectomy punch (Kelly Descemet's membrane punch) with a 1.0 mm diameter head and a 0.75 mm deep bite was inserted through a clear cornea perforated by a SatinSlit 3.2 mm angled slit knife into a viscoelastic-filled anterior chamber. The Kelly punch was placed over the lesion and pressed ...
Tópico(s): Ear and Head Tumors
2007 - BMJ | British Journal of Ophthalmology