Pyrophosphate‐dependent 6‐phosphofructo‐1‐phosphotransferase (PFP) consists of α (regulatory) and β (catalytic) subunits. The α‐subunit was previously reported to be much more susceptible to tryptic digestion than the β‐subunit. In this study, ligand‐induced protection of PFP subunits against proteolysis by subtilisin was investigated in vitro and the data obtained demonstrated that fructose 1,6‐bisphosphate (Fru‐1,6‐P 2 ), while exerting negligible effect on the β‐subunit, remarkably protected the α‐subunit ...
Tópico(s): Glycogen Storage Diseases and Myoclonus
1999 - Wiley | FEBS Letters
Zhen-Hua Yong, Genyun Chen, Shi Jiaonai, Da-Quan Xu,
It has been generally proved impossible to reassemble ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from fully denatured subunits in vitro in higher plant, because large subunit of fully denatured Rubisco is liable to precipitate when the denaturant is removed by common methods of direct dilution and one-step dialysis. In our experiment, the problem of precipitation was resolved by an improved gradual dialysis method, which gradually decreased the concentration of denaturant. However, ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2006 - Oxford University Press | Acta Biochimica et Biophysica Sinica
Hu Zhiyuan, Zha Jingjuan, Minxian Wu, Shi Jiaonai,
Tópico(s): Nitrogen and Sulfur Effects on Brassica
1994 - Oxford University Press | Acta Biochimica et Biophysica Sinica
Dong Longying, Jun Lin, Shi Jiaonai,
Phosphoenolpyruvate carboxylase (PEPCase) is one of the key enzymes in C_(4)-dicarboxylic assimilation, and an important target for genetic manipulation for increasing photosynthetic efficiency in higher plants. For introducing PEPCase gene into C_(3) a plant, we preliminarily selected the T7 RNA polymerase/promoter system, a very usefully expression plasmid, to express PEPCase cDNA in E. coli, and tried to find out the optimum conditions for its expression in higher plants. pPEP3055 plasmid was ...
Tópico(s): Photosynthetic Processes and Mechanisms
1993 - Elsevier BV | Zhiwu shengwu yu fenzi shengwuxue xuebao
Tópico(s): Food Quality and Safety Studies
1987 - Springer Science+Business Media | Science China Chemistry