Hui Zhang, Angelo D’Alessandro, Min Li, Julie A. Reisz, Suzette Riddle, Akshay Muralidhar, Todd Bull, Lan Zhao, Evgenia Gerasimovskaya, Kurt R. Stenmark,
Rationale: Sildenafil, a well-known vasodilator known to interfere with purinergic signaling through effects on cGMP, is a mainstay ... in this study we aimed to investigate if sildenafil, beyond its well-known vasodilator role in smooth ... 27 PH patients before and after treatment with sildenafil, demonstrated a partial, but specific effect of sildenafil on purine metabolites, especially adenosine, adenine, and xanthine. ... were only decreased in a small subset of sildenafil-treated patients.To better understand potential effects of ...
Tópico(s): Vascular Anomalies and Treatments
2023 - Elsevier BV | Vascular Pharmacology
Abdullah H Al Ibrahim, Khalid Q Ghallab, Fatima I. Alhumaid, Husain H Almahfoudh, Ali J Almadan, Mohammed A Al Eid, Mujtaba AlMishqab, Mohammed F Alsaffar, Jawad H Aljamea,
Sildenafil is a medication used for the treatment of erectile dysfunction. It was approved by the U. ... articles have raised concerns regarding the use of sildenafil and the occurrence of serious adverse events, such ... review the existing literature on mortality associated with sildenafil use. The method used for this systematic review ... articles found to address the concerns associated with sildenafil and mortality. A total of 19 reports were ... reports to the FDA on the use of sildenafil eight months after its introduction to the market ...
Tópico(s): Pharmaceutical Quality and Counterfeiting
2022 - Cureus, Inc. | Cureus
Trond J. Cooper, John G.F. Cleland, Marco Guazzi, Pierpaolo Pellicori, Tuvia Ben Gal, Offer Amir, Abdallah Al‐Mohammad, Andrew L. Clark, Alex McConnachie, Kjetil Steine, Kenneth Dickstein,
... with a substantial symptom burden and poor prognosis. Sildenafil, a phosphodiesterase‐5 (PDE‐5) inhibitor, might have ... to determine the safety, tolerability, and efficacy of sildenafil in patients with HFrEF and indirect evidence of PHT. Methods and results The Sildenafil in Heart Failure (SilHF) trial was an investigator‐ ... assigned in a 2:1 ratio to receive sildenafil (up to 40 mg three times/day) or ... 210 participants but, due to problems with supplying sildenafil/placebo and recruitment, only 69 patients (11 women, ...
Tópico(s): Cardiovascular Issues in Pregnancy
2022 - Elsevier BV | European Journal of Heart Failure
Su‐jin Rhee, Seung Han Shin, Jaeseong Oh, Young Hwa Jung, Chang Won Choi, Han‐Suk Kim, Kyung‐Sang Yu,
Abstract Sildenafil is widely used off-label in pediatric patients with pulmonary arterial hypertension (PAH). This study was conducted to characterize the pharmacokinetics (PK) of sildenafil in term and preterm neonates with PAH, by ... A population PK modelling analysis was performed using sildenafil and its metabolite N-desmethyl sildenafil (DMS) concentration data from 19 neonates with PAH, ... gestational ages ranged 24–41 weeks. They received sildenafil orally at a dose of 0.5–0. ... four times a day. To investigate the appropriate sildenafil dose, simulations were conducted according to body weight ...
Tópico(s): Heart Failure Treatment and Management
2022 - Nature Portfolio | Scientific Reports
Ghada S. El‐Tanbouly, Rehab S. Abdelrahman,
... current work explored the influences of time dependent Sildenafil (SILD) administration, and the possible outcomes from its ... intrarectally, 2 days before sacrification. SILD + AA, received sildenafil (25 mg/kg, orally) for 6 days starting ... pre-injection of AA; SILD-t + AA, received sildenafil (25 mg/kg, orally), starting at time of ... of AA injection; SILD-t + DEXA + AA, received sildenafil (25 mg/kg, orally) and dexamethasone (2 mg/kg, i.p.), as mentioned. Sildenafil markedly ameliorated disease activity index (DAI), ulcer scores, ...
Tópico(s): IL-33, ST2, and ILC Pathways
2022 - Springer Nature | Inflammopharmacology
Céline Baron‐Menguy, Arnaud Bocquet, Alexis Richard, Anne‐Laure Guihot, Bertrand Toutain, Pierre Pacaud, Céline Fassot, Gervaise Loirand, Daniel Henrion, Laurent Loufrani,
... play a major role in revascularization following ischemia. Sildenafil inhibits phosphodiesterase-5, increases intracellular cGMP and induces ... incompletely understood. Thus, we investigated the effect of sildenafil on post-ischemic revascularization. The left femoral artery was ligated in control and sildenafil-treated (25 mg/kg per day) rats. Vascular ... post-ligation, respectively, and was significantly increased in sildenafil-treated rats to 47 ± 4% and 128 ± 11%, ... 0.48-fold) in control without effect in sildenafil-treated animals. Blood flow and arteriolar density followed ...
Tópico(s): Protease and Inhibitor Mechanisms
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Bruce A. Berkowitz, Robert H. Podolsky, Karen Lins Childers, Aicha Saadane, Timothy S. Kern, Robin Roberts, Hailey Olds, Joydip Joy, Collin Richards, Tilman Rosales, Michael Schneider, Brennan Schilling, Arthur Orchanian, Emma Graffice, Kenan Sinan, Haohua Qian, Lamis Harp,
Purpose The phosphodiesterase inhibitor sildenafil is a promising treatment for neurodegenerative disease, but it can cause oxidative stress in photoreceptors ex vivo and degrade visual ... we test the hypotheses that in wildtype mice sildenafil causes i) wide-spread photoreceptor oxidative stress in ... In dark or light-adapted C57BL/6 mice ± sildenafil treatment, the presence of oxidative stress was evaluated ... Results In light-adapted mice, 1 hr post-sildenafil administration, oxidative stress was most evident in the ...
Tópico(s): Photoreceptor and optogenetics research
2021 - Public Library of Science | PLoS ONE
Reza Sari Motlagh, Mohammad Abufaraj, Lin Yang, Keiichiro Mori, Benjamin Pradère, Ekaterina Laukhtina, Hadi Mostafaei, Victor M. Schuettfort, Fahad Quhal, Francesco Montorsi, Mohsen Amjadi, Christian Gratzke, Shahrokh F. Shariat,
... CrI 1.24–29.8) and 100 mg sildenafil regular doses, ie once daily or nightly (OR ... recovery. The certainty of results for 100 mg sildenafil regular dose was moderate, while pelvic floor muscle ... were reported in any of the studies. Conclusions: Sildenafil 100 mg regular dose is the best penile ... Scholar 9. : Effect of starting penile rehabilitation with sildenafil immediately after robot-assisted laparoscopic radical prostatectomy on ... placebo-controlled trial of on-demand vs. nightly sildenafil citrate as assessed by Rigiscan and the international ...
Tópico(s): Genital Health and Disease
2021 - Lippincott Williams & Wilkins | The Journal of Urology
Christine M. Pierce, Min H. Zhang, Baldvin Jónsson, Dinu Iorga, N. Cheruvu, Cecile C. Balagtas, Robin H. Steinhorn,
To investigate the efficacy and safety of sildenafil added to inhaled nitric oxide (iNO) for newborn infants with persistent pulmonary hypertension of newborn (PPHN) or hypoxic respiratory failure (HRF) at risk ... 60, were randomized (1:1) to intravenous (IV) sildenafil (loading: 0.1 mg/kg, over 30 minutes; ... 87 infants screened, 29 were randomized to IV sildenafil and 30 to placebo; 13 discontinued treatment (sildenafil, n = 6; placebo: n = 7), including 3 deaths (sildenafil: n = 2; placebo: n = 1). Treatment failure rates ...
Tópico(s): Pulmonary Hypertension Research and Treatments
2021 - Elsevier BV | The Journal of Pediatrics
Jong Mok Park, Ju Hyun Shin, Seung Woo Yang, Ji Yong Lee, Chung Lyul Lee, Jae Sung Lim, Ki Hak Song, Gun‐Hwa Kim, Yong Gil Na,
Although metformin and sildenafil can protect various organs against ischemia/reperfusion (I/R) injuries, their effects and mechanisms of action in bladder I/ ... effects and mechanisms of action of metformin and sildenafil against bladder I/R insults in rats.One ... and bladder I/R groups treated with metformin, sildenafil, or both agents. Ischemia was induced by clamping ... mg/kg metformin and/or 1-mg/kg sildenafil.I/R injuries induced increased malondialdehyde levels and ... were attenuated by treatment with metformin and/or sildenafil. The I/R group had significantly higher Jun ...
Tópico(s): Cardiac Ischemia and Reperfusion
2021 - Korean Continence Society | International Neurourology Journal
Felix R. De Bie, Francesca Maria Russo, Pieter Van Brantegem, Barbara E. Coons, James K. Moon, Zexuan Yang, Chengcheng Pang, Janaína Campos Senra, Camilla Omann, Pieter Annaert, Karel Allegaert, Marcus G. Davey, Alan W. Flake, Jan Deprest,
Maternal transplacental administration of sildenafil is being considered for a variety of fetal conditions. Clinical translation also requires evaluation of fetal safety in a higher ... accessible and allows for direct fetal administration of sildenafil.Five fetal lambs were placed on extracorporeal support ... the EXTEND device and received continuous intravenous (IV) sildenafil (0.3-0.5-0.7 mg/kg/ ... a duration of one to seven days. Plasma sildenafil concentrations were sampled at regular intervals to establish ...
Tópico(s): Pregnancy and preeclampsia studies
2021 - Elsevier BV | Biomedicine & Pharmacotherapy
Mohammed Murtadha, Mohamed Raslan, Sarah Fahmy, Nagwa A. Sabri,
Sildenafil citrate, a widely-used oral therapy for erectile dysfunction, is a cytochrome P3A4 (CYP3A4) enzyme substrate. ... smokers, which predominantly mediate the hepatic elimination of sildenafil. Cigarette and/or cannabis smoking could therefore alter the exposure of sildenafil. The aim of this study was to examine ... on the pharmacokinetics, pharmacodynamics, safety and tolerability of sildenafil. Thirty-six healthy human subjects were equally divided ... Each group was administered a single dose of sildenafil (50 mg tablets). The primary outcome measures included ...
Tópico(s): Pharmacological Effects and Toxicity Studies
2021 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Eva Ausó, Violeta Gómez‐Vicente, Gema Esquiva,
Phosphodiesterase type 5 (PDE5) inhibitors such as Viagra® (sildenafil citrate) have demonstrated efficacy in the treatment of ... in rod and cone photoreceptors. The effects of sildenafil on the visual system have been investigated in ... to the fact that a high dose of sildenafil may cause mild and transient visual symptoms in ... on the pathophysiology of visual disorders induced by sildenafil. The aim of this review was not only to gather and summarize the information available on sildenafil clinical trials (CTs), but also to spot subpopulations ...
Tópico(s): Glaucoma and retinal disorders
2021 - Multidisciplinary Digital Publishing Institute | Biomedicines
... delve into the efficacy of PDE5 inhibitors including sildenafil, tadalafil and vardenafil. Used predominantly in the treatment ... to the therapeutic value of PDE5 inhibitors, particularly sildenafil and tadalafil, in the management of vascular sequelae ... commonly for the treatment of male erectile dysfunction, sildenafil is a PDE5 inhibitor that was originally introduced ... this lied within the positive regulatory effects of sildenafil on the NO/cGMP pathway, later observed with ... lung, the primary organ involved in COVID-19, sildenafil and tadalafil have also been approved by the ...
Tópico(s): COVID-19 Clinical Research Studies
2021 - Future Medicine | Future Cardiology
Jürgen Behr, Steven D. Nathan, Wim Wuyts, Nesrin Moğulkoç, Demosthenes Bouros, Katerina M. Antoniou, Julien Guiot, Mordechai R. Kramer, Klaus-Uwe Kirchgaessler, Monica Bengus, Frank Gilberg, Andras Perjesi, Sergio Harari, Athol U. Wells,
The benefit of sildenafil in patients with advanced idiopathic pulmonary fibrosis (IPF) at risk of poor outcomes from pulmonary hypertension, whether already present ... aimed to assess the efficacy and safety of sildenafil added to pirfenidone versus placebo added to pirfenidone ... Patients were randomly assigned 1:1 to oral sildenafil tablets (20 mg three times daily) or placebo, ... randomly assigned to a treatment group (n=88 sildenafil; n=89 placebo) and were assessed for the ... with disease progression over 52 weeks between the sildenafil (64 [73%] of 88 patients) and placebo groups ( ...
Tópico(s): Respiratory and Cough-Related Research
2020 - Elsevier BV | The Lancet Respiratory Medicine
Apichart Atipairin, Charisopon Chunhachaichana, Titpawan Nakpheng, Narumon Changsan, Teerapol Srichana, Somchai Sawatdee,
Sildenafil citrate is used to treat mild to moderate erectile dysfunction and premature ejaculation. However, it has ... resolved by transdermal delivery to the penis. Hence, sildenafil citrate was formulated as a microemulsion system using ... microemulsion was 2% w/w poloxamer 188. The sildenafil microemulsion-loaded hydrogels were characterised for their appearance, particle size, pH, spreadability, swelling index, viscosity, sildenafil drug content, membrane permeation, epithelial cell cytotoxicity, and ...
Tópico(s): Ocular Surface and Contact Lens
2020 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Yuan Lv, Binyu Luo, Robert R. LaBadie, Hua Zhu, Yan Feng, Cynthia C. Ernst, Penelope Crownover, Yali Liang, Qinying Zhao,
Abstract Sildenafil citrate is approved to treat erectile dysfunction. An orally disintegrating tablet (ODT) of sildenafil citrate that does not require swallowing or administration ... has been developed. The bioequivalence and bioavailability of sildenafil citrate ODT (50 mg) without and with water were compared with conventional sildenafil citrate tablets (50 mg) in an open‐label, ... n = 36) were allocated to 1 of 6 sildenafil citrate treatment sequences under fasted conditions, and plasma samples for determination of sildenafil concentrations were collected predose through 14 hours postdose. ...
Tópico(s): Marriage and Sexual Relationships
2020 - Wiley | Clinical Pharmacology in Drug Development
Fariba Hassanvand, Tannaz Mohammadi, Negar Ayoubzadeh, Atieh Tavakoli, Naiemeh Hassanzadeh, Nafiseh Sadat Sanikhani, Ali Ipakchi Azimi, Hamid Reza Mirzaei, Mehdi Khodamoradi, Kazem Abbaszadeh Goudarzi, Hossein Pourghadamyari, Mohamad Ali Zaimy,
... In this study, the antitumor activity of the sildenafil as a PDE-5 inhibitor alone and in ... MCF-7 and MDA-MB-468 was assessed.Sildenafil as PDE type 5 (PDE5) inhibitor is the ... treated by cisplatin in the presence/absence of sildenafil. Cell viability, apoptosis, and reactive oxygen species (ROS) ... cell line, following 24 h incubation, combinations of sildenafil with cisplatin (P < 0.001) showed decreased cell viability when compared to sildenafil and cisplatin alone. Moreover in MDA-MB-468 ... with cisplatin, in the presence or absence of sildenafil. However, following 48 h incubation, combinations of cisplatin ...
Tópico(s): Histone Deacetylase Inhibitors Research
2020 - BioMed Central | Journal of Cancer Research and Therapeutics
Ruchit Patel, Shyam Kumar Singh Thingnam, Anand Kumar Mishra, Manoj Kumar Rohit, V Upadhyay, Vikram Halder, Vidur Bansal,
... defect. It increases perioperative mortality and morbidity. Oral sildenafil is an effective pulmonary vasodilator. In this study, we assessed effects of perioperative oral sildenafil therapy on pulmonary artery pressure and early surgical ... hypertension were divided into two groups. In the sildenafil group (n = 15, mean age 23.3 months), oral sildenafil was administered two weeks before surgery. In the ... group (n = 15, mean age 36 months), preoperative sildenafil was not given. Sildenafil was continued postoperatively in ...
Tópico(s): Congenital Heart Disease Studies
2020 - Wiley | Journal of Cardiac Surgery
Ryota Akabane, A. Sakatani, Mizuki Ogawa, M. Nagakawa, Hirosumi Miyakawa, Yuichi Miyagawa, Naoyuki TAKEMURA,
The effects of different doses of orally administered sildenafil on pulmonary haemodynamics in dogs with pulmonary hypertension ( ... this study, we examined the effects of oral sildenafil using a canine model of chronic embolic PH ( ... received 1, 2 or 4 mg/kg of sildenafil orally twice a day for seven days. Then, ... output (CO) were obtained after seven days of sildenafil administration via right heart catheterisation and oscillometric blood pressure measurements. Sildenafil was well tolerated in this study. Sildenafil administered ...
Tópico(s): Transplantation: Methods and Outcomes
2020 - Elsevier BV | Research in Veterinary Science
Fieke Terstappen, Anne Richter, A. Titia Lely, Freek E. Hoebeek, Ayten Elvan‐Taşpınar, Arend F. Bos, Wessel Ganzevoort, Anouk Pels, Petra Lemmers, Elisabeth M. W. Kooi,
Objective: Sildenafil is under investigation as a potential agent to improve uteroplacental perfusion in fetal growth restriction (FGR). However, ... rates of persistent pulmonary hypertension and mortality in sildenafil-exposed neonates. This hypothesis-generating study within the ... women with FGR received 25 mg placebo or sildenafil thrice daily within the Dutch STRIDER trial. We ... and interaction with time (slope) between placebo- and sildenafil-exposed groups were tested using mixed effects analyses ... vs. 77%; both n = 14) with comparable slopes. Sildenafil-exposed infants ( n = 5) showed lower renal rSO ...
Tópico(s): Pregnancy and preeclampsia studies
2020 - Frontiers Media | Frontiers in Pediatrics
Simone Schneider, Mary Bailey, Tracy Spears, Charles R. Esther, Matthew M. Laughon, Christoph P. Hornik, Wesley Jackson,
... the prevention of pulmonary hypertension in this population. Sildenafil is a potent pulmonary vasodilator approved by the ... adults. Preclinical models suggest a beneficial effect of sildenafil on premature lungs through improved alveolarization and preserved vascular development. Sildenafil may therefore prevent the development of pulmonary hypertension ... generate safety, pharmacokinetics, and preliminary effectiveness data on sildenafil in a population of premature infants with severe ... sequential dose-escalating, double-masked, safety trial of sildenafil in premature infants with severe bronchopulmonary dysplasia. We ...
Tópico(s): Respiratory Support and Mechanisms
2020 - BioMed Central | BMC Pediatrics
... phosphodiesterase 5 (PDE5), which degrades cGMP, is upregulated. Sildenafil inhibits PDE5 and increases cGMP levels. Integrating previous findings, we determine that most doses of sildenafil (especially low doses) likely activate peroxisome proliferator-activated ... and PGC1α deacetylation. Via PGC1α signaling, low-dose sildenafil likely suppresses β-secretase 1 expression and amyloid- ... upregulates antioxidant enzymes, and induces mitochondrial biogenesis. Plus, sildenafil should increase brain perfusion, insulin sensitivity, long-term ...
Tópico(s): Cholinesterase and Neurodegenerative Diseases
2020 - IOS Press | Journal of Alzheimer s Disease Reports
Lauren J. Lee, Terence A. Maguire, Martine C. Maculaitis, Birol Emir, Vicky W. Li, Mara Jeffress, Jim Z. Li, Kelly H. Zou, Shaantanu Donde, David Taylor,
... Agency in the United Kingdom (UK) formally reclassified sildenafil citrate 50 mg tablets as a pharmacy medicine (sildenafil-P) in 2017 for adult men with erectile ... life (QoL) before and after the availability of sildenafil-P. Methods Adult men with ED aged ≥18 ... provided data at baseline (prior to launch of sildenafil-P) and every 3 months after the launch. ... models were used to assess the association of sildenafil-P use with total physician/nurse practitioner and ... 001) at 12 months were significantly higher among sildenafil-P users than those who never used sildenafil- ...
Tópico(s): Hormonal and reproductive studies
2020 - Wiley | International Journal of Clinical Practice
The illegal adulteration of sildenafil in herbal food supplements and alcoholic drinks immensely threatens human health due to its harmful side-effects. Therefore, it is ... to accurately detect and identify the presence of sildenafil in alcoholic drinks. In this study, Opto Trace ... enhanced Raman spectroscopy (SERS) active colloids to detect sildenafil. The results demonstrated that the Raman enhancement factor ( ... 107 and the limits of detection (LODs) of sildenafil in health wine and liquor were found to ...
Tópico(s): Hormonal and reproductive studies
2019 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Han‐Mo Yang, Sooryeonhwa Jin, Hyunduk Jang, Ju‐Young Kim, Jooeun Lee, Joonoh Kim, Hyo‐Soo Kim,
Abstract Sildenafil is known to reduce cardiac hypertrophy through cGMP-dependent protein kinase (cGK) activation. Studies have demonstrated that ... to examine the effects of cGK activation by sildenafil on neointimal formation and platelet aggregation. After vascular ... rat carotid arteries was significantly reduced in the sildenafil-treated group. This effect of sildenafil was accompanied by the reduction of viability and ... experiments showed that the increased cGK activity by sildenafil inhibited platelet-derived growth factor-induced phenotype change ...
Tópico(s): Platelet Disorders and Treatments
2019 - Nature Portfolio | Scientific Reports
Produção Nacional
Revisado por pares
Marcos André Soares Leal, Rafaela Aires, Thamirys Pandolfi, Vinícius Bermond Marques, Bianca Prandi Campagnaro, Thiago de Melo Costa Pereira, Silvana S. Meyrelles, Manuel Campos‐Toimil, Elisardo C. Vasquez,
... to investigate and elucidate the possible mechanisms of sildenafil, a selective phosphodiesterase-5 inhibitor, actions on endothelial ... from spontaneously hypertensive rats (SHR). SHR treated with sildenafil (40 mg/kg/day, p.o., 3 weeks) ... used for structural analysis of the endothelial surface. Sildenafil reduced high SBP and partially restored the vasodilator ... prostanoids contribution in the endothelium-dependent vasodilation in sildenafil-treated SHR compared to non-treated SHR. Also, the relaxant responses to sildenafil and 8-Br-cGMP were normalized in sildenafil- ...
Tópico(s): Renin-Angiotensin System Studies
2019 - Elsevier BV | Vascular Pharmacology
Yu Ueda, Lynelle R. Johnson, Eric S. Ontiveros, Lance C. Visser, Catherine T. Gunther‐Harrington, Joshua A. Stern,
... morbidity and mortality in both humans and dogs. Sildenafil, a phosphodiesterase-5 (PDE5) inhibitor causing accumulation of ... could contribute to variability in the efficacy of sildenafil. In this study, response to sildenafil therapy was evaluated in dogs with PH by ... score, and plasma cGMP concentrations before and after sildenafil therapy. Overall, tricuspid regurgitation estimated systolic pressure gradient (PG) and QOL score were significantly improved after sildenafil therapy, and the plasma cGMP concentration was significantly ...
Tópico(s): Phosphodiesterase function and regulation
2019 - Nature Portfolio | Scientific Reports
Basu Chakrabarty, Hiroki Ito, Manuela Ximenes, Nobuyuki Nishikawa, Bahareh Vahabi, Anthony Kanai, Anthony E. Pickering, Marcus J. Drake, Christopher H. Fry,
PDE inhibitors such as sildenafil alleviate lower urinary tract symptoms; however, a complete understanding of their action on the bladder remains unclear. We are investigating the effects of sildenafil, on post and preganglionic nerve-mediated contractions of ... strips, was measured using a luciferin-luciferase assay.Sildenafil (20 μM) inhibited nerve-mediated contractions in young ... relationships, demonstrating a greater effect at low frequencies. Sildenafil reduced the atropine-resistant, purinergic component of nerve- ...
Tópico(s): Adenosine and Purinergic Signaling
2019 - Wiley | British Journal of Pharmacology