J. Lewis Smith, H. G. C. Rose, Addinell Hewson, A. Charpentier, Joseph H. Wythe, Henry G. Landis, A. Charpentier, Ernst Ziegler, Frederick P. Henry, Richard Douglas, John W. McAlister, Barton Pitts, E. H. Hill, T. S. Robertson,
... Still in the Middle of the Road near Staufen, in Untermunsterthal, A Text-Book of Pathological Anatomy ...
1887 - Gale Group | NCCO-STM 1of2
Michaela Monshausen, Monika Rehbein, Dietmar Richter, Stefan Kindler,
... neurones, homologues of the Drosophila RNA‐binding protein Staufen are part of ribonucleoprotein complexes that move bidirectionally ... and translation. In this study, putative components of Staufen granules were identified in a yeast two‐hybrid ... a rat brain cDNA library with a rat Staufen bait. Protein phosphatase‐1 was found as an ... sequence motif (R‐K‐V‐T‐F) in Staufen that is conserved in a number of proteins ... both proteins was shown by coimmunoprecipitation of rat Staufen and protein phosphatase‐1 from the cytoplasm of ...
Tópico(s): Genomics and Chromatin Dynamics
2002 - Wiley | Journal of Neurochemistry
Michael Doyle, Michael Kiebler,
... contribution to dendritic mRNA localization include ZBP1, the Staufen proteins, fragile × mental retardation protein (FMRP), the cytoplasmic ... growth cone turning (Sasaki et al, 2010). The Staufen proteins are some of the best-known proteins involved in mRNA localization in many species. Staufen orthologues have been implicated in mRNA transport in ... sea mollusc Aplysia and vertebrates (St Johnston, 2005). Staufen proteins belong to the family of double-stranded ... St Johnston et al, 1992). In mammals, two Staufen proteins exist: a ubiquitously expressed Staufen 1 (Stau1) ...
Tópico(s): RNA and protein synthesis mechanisms
2011 - Springer Nature | The EMBO Journal
Staufen is a double-stranded RNA-binding protein that forms RNA granules by RNA-dependent and -independent interactions. Staufen was initially described in Drosophila as a key ... transport, thus affecting neuron plasticity, learning and memory. Staufen also regulates translation and mRNA decay. In recent years, Staufen was shown to be an important regulatory component ... reprogramming translation and inhibiting pro-apoptotic pathways, and Staufen appears to negatively modulate SG formation by several mechanisms. More recently, mammalian Staufen was found in RNA granules and pathological cytoplasmic ...
Tópico(s): RNA regulation and disease
2011 - IMR Press | Frontiers in Bioscience-Scholar
Michael Kiebler, Gary J. Bassell,
... localization in polarized cells. One key player is Staufen, a double-stranded RNA binding protein that plays ... colleagues were the first to express the mammalian Staufen homolog tagged with GFP in hippocampal neurons and to observe the MT-dependent recruitment of Staufen into RNA granules, which exhibited rapid bidirectional movements ... PDF PubMed Scopus (296) Google Scholar). In addition, Staufen and hnRNP-A2 were identified as also being ... a number of RNA binding proteins, such as Staufen and SYNCRIP, which was shown by Bannai and ...
Tópico(s): RNA modifications and cancer
2006 - Cell Press | Neuron
Staufen is a double-stranded RNA-binding protein that forms RNA granules by RNA-dependent and - independent interactions. Staufen was initially described in Drosophila as a key ... transport, thus affecting neuron plasticity, learning and memory. Staufen also regulates translation and mRNA decay. In recent years, Staufen was shown to be an important regulatory component ... reprogramming translation and inhibiting pro-apoptotic pathways, and Staufen appears to negatively modulate SG formation by several mechanisms. More recently, mammalian Staufen was found in RNA granules and pathological cytoplasmic ...
Tópico(s): RNA regulation and disease
2012 - IMR Press | Frontiers in Bioscience-Scholar
María Gabriela Thomas, Leandro Martínez Tosar, Mariela Loschi, Juana M. Pasquini, Jorge Correale, Stefan Kindler, Graciela L. Boccaccio,
Staufen is a conserved double-stranded RNA-binding protein required for mRNA localization in Drosophila oocytes and embryos. The mammalian homologues Staufen 1 and Staufen 2 have been implicated in dendritic RNA targeting ... processes. A third kind of RNA granules lacks Staufen and contains major myelin mRNAs. Myelin Staufen granules associate with microfilaments and microtubules, and their ... by polysome-disrupting drugs. Under oxidative stress, both Staufen 1 and Staufen 2 are recruited into stress ...
Tópico(s): RNA regulation and disease
2004 - American Society for Cell Biology | Molecular Biology of the Cell
Laurent Chatel‐Chaix, Jean-François Clément, Catherine Martel, Véronique Bériault, Anne Gatignol, Luc DesGroseillers, Andrew J. Mouland,
Staufen is a host protein that is selectively incorporated into human immunodeficiency virus type 1 (HIV-1) ... proviruses harboring wild-type or mutant forms of Staufen were expressed in 293T cells. Cell fractionation analyses demonstrated that Staufen cosedimented with pr55Gag within detergent-resistant, trypsin-sensitive ... assays demonstrated a specific and direct interaction between Staufen and the nucleocapsid domain of pr55Gag in vitro ... interaction is shown here to be mediated by Staufen's dsRBD3, with a contribution from its C- ...
Tópico(s): Immune Cell Function and Interaction
2004 - Taylor & Francis | Molecular and Cellular Biology
Paolo Macchi, Amy M. Brownawell, Barbara Grunewald, Luc DesGroseillers, Ian G. Macara, Michael Kiebler,
The mammalian double-stranded RNA-binding proteins Staufen (Stau1 and Stau2) are involved in RNA localization in polarized neurons. In contrast to the more ubiquitously expressed Stau1, Stau2 is mainly expressed ... third double-stranded RNA-binding domain (RBD3) of Staufen is essential for RNA interaction. When conserved amino ... destination. The mammalian double-stranded RNA-binding proteins Staufen (Stau1 and Stau2) are involved in RNA localization ... third double-stranded RNA-binding domain (RBD3) of Staufen is essential for RNA interaction. When conserved amino ...
Tópico(s): Nuclear Structure and Function
2004 - Elsevier BV | Journal of Biological Chemistry
... March 2000free access Distinct roles of two conserved Staufen domains in oskar mRNA localization and translation David ... favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Drosophila Staufen protein is required for the localization of oskar ... mRNA in dividing neuroblasts. The only regions of Staufen that have been conserved throughout animal evolution are ... dsRNA when the insertion is removed. Full-length Staufen protein lacking this insertion is able to associate ...
Tópico(s): Silk-based biomaterials and applications
2000 - Springer Nature | The EMBO Journal
Andres Ramos, Stefan Grünert, Jan Adams, David Micklem, Mark R. Proctor, Stefan M.V. Freund, Mark Bycroft, Daniel St Johnston, Gabriele Varani,
Article1 March 2000free access RNA recognition by a Staufen double-stranded RNA-binding domain Andres Ramos Andres ... we have studied the third dsRBD from Drosophila Staufen. The domain binds optimally to RNA stem–loops ... this interaction. By mutating these residues in a staufen transgene, we show that the RNA-binding activity of dsRBD3 is required in vivo for Staufen-dependent localization of bicoid and oskar mRNAs. Using ... dsRBD was first identified in the Drosophila protein Staufen, which contains five copies of this motif (St ...
Tópico(s): RNA Research and Splicing
2000 - Springer Nature | The EMBO Journal
Alejandra Gardiol, Daniel St Johnston,
... show that the evolutionary conserved dsRNA binding protein, Staufen, localises to the postsynaptic side of the Drosophila ... for the localisation of coracle mRNA and protein. Staufen plays a well-characterised role in the localisation of oskar mRNA to the oocyte posterior, where Staufen dsRNA-binding domain 5 is specifically required for its translation. Removal of Staufen dsRNA-binding domain 5, disrupts the postsynaptic accumulation ... affecting the localisation of cora mRNA, suggesting that Staufen similarly regulates Coracle translation. Tropomyosin II, which functions ...
Tópico(s): Neurobiology and Insect Physiology Research
2014 - Elsevier BV | Developmental Biology
Kirsten Hanke, Oliver Höhn, Linda Liedgens, Katharina Fiddeke, Jula Wamara, Reinhard Kurth, Norbert Bannert,
... two-hybrid approach, we have newly identified human Staufen-1 as a Rec-interacting protein. The interaction ... by coimmunoprecipitation experiments, and the relevant site in Staufen-1 has been mapped to double-stranded RNA binding domain 4 (RBD4). Staufen-1 is in several aspects functionally related to ... for efficient translation. We observed an accumulation of Staufen-1 in the nucleus of Rec-expressing cells ... as well as in the cytoplasm. Overexpression of Staufen-1 resulted in a 5-fold enhancement in ...
Tópico(s): RNA Research and Splicing
2013 - American Society for Microbiology | Journal of Virology
... to clone and characterize the posterior group gene staufen, which Trudi Schüpbach had shown to be the ... Schüpbach and Wieschaus, 1986). The embryos produced by staufen mutant females have a reduced head that often ... other posterior group genes, vasa, valois and tudor, staufen is named after a royal family that died ... lack of offspring, in this case the Hohen Staufen, the most famous of whom was the Holy ... investigate the cause of the head defects in staufen mutants, and the obvious place to start was ... that extends towards the middle, which explains why staufen mutants lack the anterior structures that require the ...
Tópico(s): Neurobiology and Insect Physiology Research
2001 - Springer Nature | The EMBO Journal
... and prospero mRNA are partitioned to the GMC. Staufen, a dsRNA-binding protein required for localizing maternal ... GMC (10Broadus J Fuerstenberg S Doe C.Q Staufen-dependent localization of prospero mRNA contributes to neuroblast ... Miranda, is required to localize both Prospero and Staufen to the GMC. Since Prospero is independently localized ... mRNA localization gratuitous? The answer is no. While staufen mutants alone are not defective in GMC differentation, staufen is important for GMC fate, since staufen mutations ...
Tópico(s): Fungal and yeast genetics research
1998 - Cell Press | Cell
Suzanne M. Hurst, Nicholas J. Talbot, Howard Stebbings,
ABSTRACT In Drosophila melanogaster the staufen gene encodes an RNA-binding protein that is essential for the correct localization of certain nurse cell-derived transcripts in oocytes. ... the mechanism underlying mRNA localization is unknown, mRNA-staufen complexes have been shown to move in a ... dependent manner, and it has been suggested that staufen associates with a motor protein which generates the ... of large aggregates of parallel microtubules. Using a staufen peptide antibody and RNA probes we have identified ...
Tópico(s): Plant Molecular Biology Research
1999 - The Company of Biologists | Journal of Cell Science
Prashali Bansal, Johannes Madlung, Kristina Schaaf, Boris Maček, F. Bono,
... include the double-stranded-RNA-binding protein (dsRBP) Staufen (Stau) (4St Johnston D. Beuchle D. Nüsslein-Volhard C. staufen, a gene required to localize maternal RNAs in ... Identification of a novel homolog of the Drosophila staufen protein in the chromosome 8q13–q21.1 region. ... C. Ortín J. A human sequence homologue of Staufen is an RNA-binding protein that is associated ... Luo M. Nabi I.R. DesGroseillers L. Mammalian staufen is a double-stranded-RNA- and tubulin-binding ... Scholar, 8Yoon Y.J. Mowry K.L. Xenopus Staufen is a component of a ribonucleoprotein complexcontaining Vg1 ...
Tópico(s): RNA and protein synthesis mechanisms
2020 - Elsevier BV | Molecular & Cellular Proteomics
Uwe Irion, Jan Adams, Chin-Wen Chang, Daniel St Johnston,
The double-stranded RNA binding protein Staufen is required for the microtubule-dependent localization of bicoid and oskar mRNAs to opposite poles of the Drosophila oocyte and also mediates ... divisions. The posterior localization of oskar mRNA requires Staufen RNA binding domain 2, whereas prospero mRNA localization ... to RNA binding domain 5, suggesting that different Staufen domains couple mRNAs to distinct localization pathways. Here, ... the expression of Miranda during mid-oogenesis targets Staufen/oskar mRNA complexes to the anterior of the ...
Tópico(s): RNA Interference and Gene Delivery
2006 - Elsevier BV | Developmental Biology
Cornelia Brendel, Monika Rehbein, Hans‐Jürgen Kreienkamp, Friedrich Buck, Dietmar Richter, Stefan Kindler,
... and neuroblasts, the double-stranded RNA binding protein Staufen assembles into ribonucleoprotein particles, which mediate cytoplasmic mRNA ... little is known about the molecular composition of Staufen-containing ribonucleoprotein complexes. Here, we have used a ... step affinity purification protocol to identify components of Staufen 1-containing particles. Whereas the nucleocytoplasmic RNA-binding protein nucleolin is linked to Staufen in an RNA-dependent manner, the association of ... of the large and small ribosomal subunits with Staufen ribonucleoprotein complexes is RNA-independent. Notably, all these ...
Tópico(s): RNA regulation and disease
2004 - Portland Press | Biochemical Journal
Rachel Allison, Kevin Czaplinski, Anna Git, ELIZABETH ADEGBENRO, Fiona A. Stennard, Evelyn Houliston, Nancy Standart,
... early asymmetries in the developing embryo. In Drosophila , Staufen is intimately involved in the localization of maternally ... embryo. We show that double-stranded RNA-binding Staufen proteins are present in the oocytes of a ... and Vg1 mRNA become localized. We identified two Staufen isoforms named XStau1 and XStau2, where XStau1 was found to be the principal Staufen protein in oocytes, eggs, and embryos, the levels ... proteins peaking during mid-oogenesis. In adults, Xenopus Staufens are principally expressed in ovary and testis. XStau1 ...
Tópico(s): Genomics and Chromatin Dynamics
2004 - Cold Spring Harbor Laboratory Press | RNA
Michael Kiebler, Luc DesGroseillers,
... RNA-binding proteins, with a special emphasis on Staufen and its involvement in the transport of mRNAs ... Elphick L Nüsslein-Volhard C St. Johnston D Staufen protein associates with the 3′-UTR of bicoid ... RBP) and double-stranded RNA-binding proteins (especially Staufen). The striking (and surprising) outcome that homologs of ... involved in mRNA transport, and then focus on Staufen and ZBP-1, the first two proteins linked ... In NeuronsStaufenDrosophilaneuroblastprospero13Broadus J Fuerstenberg S Doe C.Q Staufen-dependent localization of prospero mRNA contributes to neuroblast ...
Tópico(s): RNA Research and Splicing
2000 - Cell Press | Neuron
... in Drosophila, the double-stranded RNA-binding protein Staufen associates with bicoid, oskar, and prospero mRNA and ... trans-acting RNA-binding proteins. In Drosophila, the Staufen protein associates with similar RNA-containing and microtubule- ... 503) Google Scholar). In mammals, several homologs of Staufen have recently been identified (7Kiebler M.A Hemraj ... 197) Google Scholar). Immunohistochemical localization of a rat Staufen homolog revealed a somatodendritic localization in cultured hippocampal ... Scholar). In vitro binding assays indicated that mouse Staufen binds all double-stranded RNAs tested (19Wickham L ...
Tópico(s): Silk-based biomaterials and applications
1999 - Cell Press | Neuron
Yoon Ki Kim, Luc Furic, Marc Parisien, François Major, Luc DesGroseillers, Lynne E. Maquat,
... how extensively the double-stranded RNA-binding protein Staufen (Stau)1 is utilized by mammalian cells to ... variety of physiologic transcripts and metabolic pathways. Introduction Staufen (Stau)1-mediated mRNA decay (SMD) is a ... ARF1 3′-UTR To date, the best characterized Staufen-binding site exists within Drosophila bicoid mRNA (Ferrandon ... Linker scanning mutations that disrupt the interaction of Staufen with this mRNA mapped to three noncontiguous regions: ... al, 1994). Given the structural complexity of this Staufen-binding site(s), we anticipated that identifying functional ...
Tópico(s): RNA and protein synthesis mechanisms
2007 - Springer Nature | The EMBO Journal
María Verónica Báez, Graciela L. Boccaccio,
... contain polyadenylated mRNA and the RNA-binding proteins Staufen, TIAR, TIA-1, and HuR. Smaug 1 foci ... contain polyadenylated mRNA and the RNA-binding proteins Staufen, TIAR, TIA-1, and HuR. Smaug 1 foci ... pcDNA3.0 vector. A pCDNA6.0 encoding murine Staufen 1 (GenBank™ accession number AF395842) (20Thomas M.G. ... 1:10,000 to 1:20,000; anti-Staufen 1 RLS1, 1:5000 (20Thomas M.G. Martinez ... As expected, the double-stranded RNA-binding protein Staufen 1 was detected in polysomes and in a ... Fig. 4F). Finally, for comparison, a construct encoding Staufen 1-V5 was cotransfected with hSmaug 1-V5 ...
Tópico(s): Genomics and Chromatin Dynamics
2005 - Elsevier BV | Journal of Biological Chemistry
Yong-Fang Wu, Yueming Zhang, He-Hua Ge, Chongyang Ren, Zhe‐Zhe Zhang, Lei Cao, Fang Wang, Gui‐Hai Chen,
... associated proteins, such as RNA-binding proteins (RBPs). Staufen is a double-stranded RBP that plays a ... whether these adverse factors can induce changes in Staufen expression, and whether these changes are correlated with ... assessed using the Morris water maze test, while Staufen expression was examined at both the protein and ... aged mice had worse cognitive performance and higher Staufen expression than young mice. Embryonic inflammation induced cognitive impairment and increased Staufen expression in the middle-aged mice, whereas adolescent ...
Tópico(s): Tryptophan and brain disorders
2020 - Frontiers Media | Frontiers in Aging Neuroscience
Nico Goldscheider, Timothy Bechtel,
... classical German literature know the historic town of Staufen (7,800 inhabitants; Fig. 1) as the town ... immediately following geothermal drillings in the city centre. Staufen is located in the Upper Rhine Graben, within ... Upper Triassic (Keuper), which is also present below Staufen, includes gypsum and anhydrite layers and lenses of ... knowledge appears to have been ignored during the Staufen project, and a predictable disaster has run its ... released in December 2008 on the website of Staufen, www.stadt-staufen.de). One type of crack ...
Tópico(s): CO2 Sequestration and Geologic Interactions
2009 - Springer Science+Business Media | Hydrogeology Journal
Paolo Macchi, Sven Kroening, Isabel M. Palacios, Simona Baldassa, Barbara Grunewald, Concetta Ambrosino, Bernhard Goetze, Andrei N. Lupas, Daniel St Johnston, Michael Kiebler,
Staufen1, the mammalian homolog of Drosophila Staufen, assembles into ribonucleoprotein particles (RNPs), which are thought to transport and localize RNA into dendrites of mature hippocampal neurons. We therefore investigated ... additional components of the RNA localization complex besides Staufen are conserved. One candidate is the mammalian homolog ... of the oskar RNA localization complex along with Staufen. In this study, we report the characterization of ... Btz is still able to interact with Drosophila Staufen and reach the posterior pole in the wild- ...
Tópico(s): Plant Molecular Biology Research
2003 - Society for Neuroscience | Journal of Neuroscience
John Laver, Xiao Li, Kristin Ancevicius, J. Timothy Westwood, Craig A. Smibert, Quaid Morris, Howard D. Lipshitz,
... interactions of double-stranded RNA-binding proteins like Staufen with RNA in vitro, how they achieve target ... co-immunoprecipitations followed by microarray analysis to identify Staufen-associated mRNAs in early Drosophila embryos.Analysis of ... revealed a number of potentially novel roles for Staufen.Using computational methods, we identified two sequence features that distinguish Staufen's target transcripts from non-targets.First, these ... unbound transcripts.Second, the 3 0 UTRs of Staufen-bound transcripts are highly enriched for three types ...
Tópico(s): RNA modifications and cancer
2013 - Oxford University Press | Nucleic Acids Research
Michaela Monshausen, Ulrich Putz, Monika Rehbein, Michaela Schweizer, Luc DesGroseillers, Dietmar Kuhl, Dietmar Richter, Stefan Kindler,
... highly homologous to the Drosophila RNA-binding protein Staufen. Mammalian Staufen appears to be expressed in most tissues and brain areas. Two distinct rat brain Staufen isoforms, rStau+I6 and rStau-I6, are encoded ... rStau+I6. Using an antibody directed against mammalian Staufen, the protein was detected in somata and dendrites ... cortex. Ultrastructural studies revealed that in dendrites, rat Staufen accumulates along microtubules. Thus in neurones, rat Staufen may serve to link RNAs to the dendritic ...
Tópico(s): RNA regulation and disease
2001 - Wiley | Journal of Neurochemistry
Alison Schuldt, Jan H.J. Adams, Catherine M. Davidson, David Micklem, Jim Haseloff, Daniel St Johnston, Andrea H. Brand,
... is similarly segregated by the dsRNA binding protein, Staufen. Here we show that Staufen interacts in vivo with a segment of the prospero 3′ UTR. Staufen protein and prospero RNA colocalize to the apical ... prophase. Both the apical and basal localization of Staufen are abolished by the removal of a conserved ... screen with Miranda protein. Furthermore, Miranda colocalizes with Staufen protein and prospero mRNA during neuroblast divisions, and neither Staufen nor prospero RNA are localized in miranda mutants. ...
Tópico(s): RNA modifications and cancer
1998 - Cold Spring Harbor Laboratory Press | Genes & Development