Era A. Izano, Suhagi Shah, Jeffrey B. Kaplan,
Respiratory infections caused by nontypeable Haemophilus influenzae (NTHi) are a major medical problem. Evidence suggests that the ability to form biofilms on mucosal surfaces may play a role in NTHi pathogenesis. However, the factors that contribute to NTHi biofilm cohesion remain largely unknown. In this study we investigated the biofilm growth and detachment phenotypes of eight NTHi clinical strains in vitro. We found that the majority of strains produced biofilms within 6 h when cultured statically ...
Tópico(s): Bacterial biofilms and quorum sensing
2009 - Elsevier BV | Microbial Pathogenesis
Krishnaraj Ganeshnarayan, Suhagi Shah, Matthew Libera, Anthony Santostefano, Jeffrey B. Kaplan,
ABSTRACT Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-β(1,6)- N -acetyl- d -glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilms produced in vitro by the gram-negative porcine respiratory pathogen Actinobacillus pleuropneumoniae and the gram-positive device-associated pathogen Staphylococcus epidermidis . ...
Tópico(s): Antimicrobial Peptides and Activities
2008 - American Society for Microbiology | Applied and Environmental Microbiology
Jie Hu, Uma N. M. Rao, Suhagi Jasani, Vineesh Khanna, Kenneth Yaw, Urvashi Surti,
Leiomyosarcomas (LMS) account for 10–20% of all soft tissue sarcomas. We analyzed 10 primary, 5 metastatic, and 2 recurrent extrauterine LMS. Genomic imbalances were detected in 15 out of the 17 tumors. The most common regions of loss were 13q (59%, 10 of 17), 10q (59%, 10 of 17), 2q (35%, 6 of 17), and 16q (29%, 5 of 17). The most common region of gain was 5p (35%, 6 of 17). High-level gain of DNA copy number was detected in 6p and 17p. Loss of function of tumor suppressor genes or the activation of ...
Tópico(s): Neurofibromatosis and Schwannoma Cases
2005 - Elsevier BV | Cancer Genetics and Cytogenetics
Sara Cohen, Norah L. Smith, Courtney E McDougal, Marion Pepper, Suhagi Shah, George Yap, Hans Acha‐Orbea, Aimin Jiang, Björn E. Clausen, Brian D. Rudd, Eric Denkers,
Beta-catenin signaling has recently been tied to the emergence of tolerogenic dendritic cells (DCs). In this article, we demonstrate a novel role for beta-catenin in directing DC subset development through IFN regulatory factor 8 (IRF8) activation. We found that splenic DC precursors express beta-catenin, and DCs from mice with CD11c-specific constitutive beta-catenin activation upregulated IRF8 through targeting of the Irf8 promoter, leading to in vivo expansion of IRF8-dependent CD8a+, plasmacytoid, ...
Tópico(s): T-cell and B-cell Immunology
2014 - American Association of Immunologists | The Journal of Immunology
Veerupaxagouda Patil, Yanlin Zhao, Suhagi Shah, Barbara A. Fox, Leah M. Rommereim, David J. Bzik, George Yap,
Pro-inflammatory M1 macrophages are critical for defense against intracellular pathogens while alternatively-activated M2 macrophages mediate tissue homeostasis and repair. Whether these distinct activation programs are mutually exclusive or can co-exist within the same cell is unclear. Here, we report the co-existence of these programs in Toxoplasma gondii-elicited inflammatory macrophages. This is independent of parasite expression of the virulence factor ROP16 and host cell expression of signal ...
Tópico(s): HIV Research and Treatment
2013 - Elsevier BV | International Journal for Parasitology
Suhagi Shah, Gijsbert M. Grotenbreg, Amariliz Rivera, George Yap,
The proinflammatory cytokine IL-12 drives the generation of terminally differentiated KLRG1+ effector CD8+ T cells. Using a Toxoplasma vaccination model, we delineate the sequence of events that naïve CD8+ T cells undergo to become terminal effectors and the differentiation steps controlled by IL-12. We demonstrate that direct IL-12 signaling on CD8+ T cells is essential for the induction of KLRG1 and IFN-γ, but the subsequent downregulation of CXCR3 is controlled by IL-12 indirectly through the ...
Tópico(s): Immune Cell Function and Interaction
2015 - eLife Sciences Publications Ltd | eLife
Andrew Marple, Wenhui Wu, Suhagi Shah, Yanlin Zhao, Peicheng Du, William C. Gause, George Yap,
Abstract Concurrent helminth infection potently inhibits T cell immunity; however, whether helminthes prevent T cell priming or skew clonal recruitment and effector differentiation is not known. Using coinfection with two natural mouse pathogens, Heligmosomoides polygyrus and Toxoplasma gondii, to investigate the negative impact of helminthes on the CD8 T cell response, we demonstrate helminth-induced suppression of IL-12–dependent differentiation of killer-like receptor G1+ effector CD8 T cells ...
Tópico(s): Toxoplasma gondii Research Studies
2016 - American Association of Immunologists | The Journal of Immunology
Mautin Hundeyin, Emma Kurz, Ankita Mishra, Juan Andres Kochen Rossi, Shannon M. Liudahl, Kenna R. Leis, Harshita Mehrotra, Mirhee Kim, Luisana E. Torres, Adesola Ogunsakin, Jason M. Link, Rosalie C. Sears, Shamilene Sivagnanam, Jeremy Goecks, Kittiya Islam, Igor Dolgalev, Shivraj Savadkar, Wei Wang, Berk Aykut, Joshua Leinwand, Brian Diskin, Salma Adam, Muhammad Israr, Maeliss Gelas, Justin Lish, Kathryn Chin, Mohammad Saad Farooq, Benjamin Wadowski, Jingjing Wu, Suhagi Shah, Dennis O. Adeegbe, Smruti Pushalkar, Varshini Vasudevaraja, Deepak Saxena, Kwok‐Kin Wong, Lisa M. Coussens, George Miller,
Abstract Unconventional T-lymphocyte populations are emerging as important regulators of tumor immunity. Despite this, the role of TCRαβ+CD4−CD8−NK1.1− innate αβ T cells (iαβT) in pancreatic ductal adenocarcinoma (PDA) has not been explored. We found that iαβTs represent ∼10% of T lymphocytes infiltrating PDA in mice and humans. Intratumoral iαβTs express a distinct T-cell receptor repertoire and profoundly immunogenic phenotype compared with their peripheral counterparts and conventional lymphocytes. ...
Tópico(s): CAR-T cell therapy research
2019 - American Association for Cancer Research | Cancer Discovery
Jiehui Deng, Aatish Thennavan, Suhagi Shah, Ece Bagdatlioglu, Natalie Klar, Adriana Heguy, Christian Marier, Peter Meyn, Yutong Zhang, Kristen Labbe, Christina Almonte, Michelle Krogsgaard, Charles M. Perou, Kwok‐Kin Wong, Sylvia Adams,
Immunotherapy has recently been shown to improve outcomes for advanced PD-L1-positive triple-negative breast cancer (TNBC) in the Impassion130 trial, leading to FDA approval of the first immune checkpoint inhibitor in combination with taxane chemotherapy. To further develop predictive biomarkers and improve therapeutic efficacy of the combination, interrogation of the tumor immune microenvironment before therapy as well as during each component of treatment is crucial. Here we use single-cell RNA ...
Tópico(s): CAR-T cell therapy research
2020 - Springer Science+Business Media | Breast Cancer Research and Treatment
Tricia J. Johnson, Paula P. Meier, Daniel T. Robinson, Sumihiro Suzuki, Suhagi Kadakia, Andrew N. Garman, Aloka L. Patel,
In the United States, 10% of infants are born preterm (PT; <37 weeks gestational age) each year and are at higher risk of complications compared to full term infants. The burden of PT birth is borne disproportionately by Black versus non-Black families, with Black mothers significantly more likely to give birth to a PT infant. One proven strategy to improve short- and long-term health outcomes in PT infants is to feed mother’s own milk (MOM; breast milk from the mother). However, mothers must make ...
Tópico(s): Work-Family Balance Challenges
2023 - Multidisciplinary Digital Publishing Institute | Children
Adel Aboul-Naga, Taha H. Abdelsabour,
... Minor local breeds are Fallahi, Wahati, Abudeleik, and Suhagi sheep located mainly in the south and in ...
Tópico(s): Livestock Management and Performance Improvement
2023 - | Journal of Animal and Poultry Production
Kayla M. Harmeyer, Jiehui Deng, Suhagi Shah, Ece Bagdatlioglu, Aarif Ahsan, Christopher M. Reid, Aatish Thennavan, Charles M. Perou, Kwok‐Kin Wong, Harvey I. Pass,
... lung cancer.Citation Format: Kayla Harmeyer, Jiehui Deng, Suhagi Shah, Ece Bagdatlioglu, Aarif Ahsan, Christopher Reid, Aatish ...
Tópico(s): Cancer Genomics and Diagnostics
2019 - SAGE Publishing | Tumor Biology
Kayla M. Harmeyer, Jiehui Deng, Suhagi Shah, Ece Bagdatlioglu, Aarif Ahsan, Christopher M. Reid, Aatish Thennavan, Charles M. Perou, Kwok‐Kin Wong, Harvey I. Pass,
... lung cancer. Citation Format: Kayla Harmeyer, Jiehui Deng, Suhagi Shah, Ece Bagdatlioglu, Aarif Ahsan, Christopher Reid, Aatish ...
Tópico(s): Cancer Genomics and Diagnostics
2019 - American Association for Cancer Research | Cancer Research
Jiehui Deng, Suhagi Shah, Aatish Thennavan, Michelle Krogsgaard, Charles M. Perou, Kwok‐Kin Wong, Sylvia Adams,
... immunotherapy for TNBC patients. Citation Format: Jiehui Deng, Suhagi Shah, Aatish Thennavan, Michelle Krogsgaard, Charles M. Perou, ...
Tópico(s): Single-cell and spatial transcriptomics
2019 - American Association for Cancer Research | Cancer Research
M. C. Agrawal, J David George, Samidha Gupta,
... of 3 991 L. Meola collected [rum Marhai, Suhagi and Gorakhpur areas of Jabalpur, 134 (3.35%) ...
Tópico(s): Helminth infection and control
2000 - Indian Council of Agricultural Research | The Indian Journal of Animal Sciences
... C. Dyrland, Charlie B. Fischer, Aloka L. Patel, Suhagi KadakiaRush University Medical CenterAbstract Category: Human Milk CompositionBackground: ...
Tópico(s): Infant Development and Preterm Care
2023 - Mary Ann Liebert, Inc. | Breastfeeding Medicine
Nicole Larsen, Tricia J. Johnson, Aloka L. Patel, Mary Dyrland, Charlie B. Fischer, Kayla Dobies, Paula P. Meier, Suhagi Kadakia,
Tópico(s): Child Nutrition and Feeding Issues
2025 - Mary Ann Liebert, Inc. | Breastfeeding Medicine
Suhagi Shah, Gijsbert M. Grotenbreg, George Yap,
Abstract The obligate intracellular parasite Toxoplasma gondii elicits a robust and uniquely IL-12 dependent CD8+ T cell immune response. We have previously shown that IL-12 is required for the differentiation of KLRG1+ CD8+ T effector cells following T. gondii infection. We have also shown that in the absence of IL-12, the frequency of KLRG1- memory precursor effector cell (MPECs) differentiation is increased at the expense of KLRG1+ short-lived effector cells (SLECs). Therefore, IL-12 may be a critical ...
Tópico(s): Immune Cell Function and Interaction
2012 - American Association of Immunologists | The Journal of Immunology
Suhagi Shah, Gijsbert M. Grotenbreg, Amariliz Rivera, George Yap,
Abstract We have previously shown that IL-12 is necessary for the differentiation of KLRG1+ IFN-γ producing CD8+ T effector cells during the immune response to Toxoplasma gondii. However, which key events during the activation, differentiation and mobilization of CD8+ T effector cells are critically regulated by IL-12 have not been identified. By tracking the dynamics of endogenous and adoptively transferred T. gondii-reactive CD8+ T cells, we were able to identify two key IL-12 dependent checkpoints. ...
Tópico(s): Cytomegalovirus and herpesvirus research
2013 - American Association of Immunologists | The Journal of Immunology
Andrew Marple, Wenhui Wu, Suhagi Shah, Zhao Yanlin, Peicheng Du, William C. Gause, George Yap,
Tópico(s): Parasites and Host Interactions
2017 - American Association of Immunologists | The Journal of Immunology
Suhagi Kadakia, Alan Cabasso, Anita Siu, Rose St. Fleur,
Tópico(s): Child Nutrition and Feeding Issues
2018 - American Academy of Pediatrics | PEDIATRICS
Deborah Linehan, Dennis Lee, Cheryl Horn, Suhagi Kadakia, Rose M. Viscardi,
Background: Oxygen therapy in the premature neonatal population is associated with negative outcomes and neonatal intensive care units (NICU) use continuous pulse oximetry to limit its use in these vulnerable subjects. The NICU at a large university hospital has a 4-pronged Target Oxygen Protocol 1) Set correct SpO2 alarm limits 2) Use Nellcor OxiMax N-600x pulse oximeter (Covidien, Mansfield, Massachusetts) to display histogram of percent time in each SpO2 quintile per 24h period 3) Display correct ...
Tópico(s): Non-Invasive Vital Sign Monitoring
2019 - American Association for Respiratory Care | Respiratory Care
Jieun David, Suhagi Kadakia, Beverley Robin,
Due to the COVID-19 pandemic, medical education training programs had to rapidly switch to a virtual interview (VI) format for the 2021 application cycle. Studies have demonstrated a gap in applicant perceptions of program culture through the VI. The objectives of this study were to assess the perceptions of culture from VIs compared to direct experience after beginning training and explore VI gaps in representing culture.
Tópico(s): Higher Education Practises and Engagement
2024 - Cureus, Inc. | Cureus
Sierra S. Donnell, Megan Kraemer, Suhagi Kadakia,
Abstract Objectives To present an unusual presentation and diagnosis of CHARGE syndrome with vocal fold paralysis, a rarely associated congenital laryngeal anomaly, as the presenting feature. Case presentation A four-day old, full-term, male infant born via uncomplicated vaginal delivery with a nursery course significant for failed hearing screen presented to an emergency department (ED) with respiratory distress and worsening stridor. He was transferred to a level III neonatal intensive care unit ( ...
Tópico(s): Congenital heart defects research
2023 - De Gruyter | Case Reports in Perinatal Medicine
Genesis Talia Mancha, Suhagi Kadakia, Lorenzo F. Muñoz, Laura M. Seske,
Background: Neurosurgical interventions in neonates may contribute to increased neonatal intensive care unit (NICU) stay. The impact of neurosurgical interventions on length of stay (LOS) and cost is not well-documented in the literature. In addition to LOS, other factors may impact overall resource utilization. Our objective was to perform a cost analysis of neonates who underwent neurosurgical procedures. Methods: A retrospective chart review was performed on NICU patients who underwent ventriculoperitoneal ...
Tópico(s): Spinal Dysraphism and Malformations
2023 - Medknow | Surgical Neurology International
Suhagi Kadakia, Amal Isaiah, Dina El‐Metwally,
Tópico(s): Infant Development and Preterm Care
2022 - Springer Nature | Pediatric Research