Project Gutenberg
Zi-Mei Peng, Xiaojian Han, Tao Wang, Jianjun Li, Chunxi Yang, Fangfang Tou, Zhen Zhang,
Abstract Background Triple-negative breast cancer (TNBC) remains the most challenging subtype of breast cancer and lacks definite treatment targets. Aerobic glycolysis is a hallmark of metabolic reprogramming that ... roles of the posttranslational modification of PFKP in TNBC remain unknown. Methods To explore whether PFKP protein has a potential role in the progression of TNBC, protein levels of PFKP in TNBC and normal breast tissues were examined by CPTAC ... used to detect the effect of PFKP on TNBC progression. To clarify the role of the USP5- ...
Tópico(s): Mitochondrial Function and Pathology
2024 - BioMed Central | Breast Cancer Research
Zhaolei Cui, Yanhong Li, Yingying Lin, Chaoqiang Zheng, Lingqing Luo, Dan Hu, Yan Chen, Zhenzhou Xiao, Yang Sun,
... lactylation expression signature in triple-negative breast cancer (TNBC) remains underexplored. Methods We conducted a comprehensive lactylproteome profiling of eight pairs of TNBC samples and their matched adjacent tissues. This was ... LA). The expression of identified lactylated proteins in TNBC was detected using immunoblotting and immunohistochemistry (IHC) with ... proteins, delineating the protein lactylation alteration signature in TNBC. Bioinformatic and functional analyses indicated that these lactylated ...
Tópico(s): RNA modifications and cancer
2024 - Frontiers Media | Frontiers in Endocrinology
Ximin Tan, Deguang Kong, Zhuoli Tao, Fangling Cheng, Baoyuan Zhang, Zaiqi Wang, Qi Mei, Chuang Chen, Kongming Wu,
Abstract Background Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype lacking effective targeted therapies, necessitating innovative treatment approaches. While targeting ROS proto-oncogene 1 (ROS1) with ... impact of FAK inhibitor IN10018 and crizotinib in TNBC and elucidate the underlying mechanisms. Methods We employed ... to analyze FAK and ROS1 mRNA levels in TNBC and adjacent normal tissues. Furthermore, we investigated the correlation between FAK, ROS1, and TNBC clinical prognosis using the GSE database. We conducted ...
Tópico(s): Cell Adhesion Molecules Research
2024 - BioMed Central | Biomarker Research
Hong Hu, Kwok Lung Tong, John Y. C. Tsang, Chia W. Ko, Frederick W.K. Tam, Thomson Loong, Gary M. Tse,
•Utilizing TNBC IHC surrogate and spatial immune patterns may be a practical means of representing gene profiling subtypes.•Surrogate TNBC subtypes showed distinct features and prognoses.•Marker expression ... breast origin of metastatic cancers varied among different TNBC subtypes. BackgroundTriple-negative breast cancer (TNBC) subtyping by gene profiling has provided valuable clinical ... Here, we aimed to evaluate the relevance of TNBC subtyping using immunohistochemistry (IHC), which could be a ... applications in patient management.MethodsA total of 123 TNBC cases were classified using androgen receptor (AR), CD8, ...
Tópico(s): Cancer Diagnosis and Treatment
2024 - Elsevier BV | ESMO Open
Yan Zhao, Yangyang Yu, Xiangmin Li, Ayao Guo,
... and immune evasion in triple-negative breast cancer (TNBC). Methods PTX resistance-related genes were screened by bioinformatics. CEBPD and VAMP3 expression in clinical TNBC samples was examined by immunohistochemistry. Three PTX-resistant TNBC cell lines (MDA-MB-231/PTX, MDA-MB- ... programmed cell death-ligand 1 (PD-L1) in TNBC cells was detected. Extracellular vesicles (EVs) from TNBC cells were isolated to examine their effects on ... chemo-resistant tissue samples and in PTX-resistant TNBC cells. The CEBPD downregulation enhanced PTX sensitivity of ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2024 - BioMed Central | Journal of Experimental & Clinical Cancer Research
Huachen Song, Zitong Zhao, Liying Ma, Weihong Zhao, Yi Hu, Yongmei Song,
Abstract Triple-negative breast cancer (TNBC) cells are in a more hypoxic and starved state than non-TNBC cells, which makes TNBC cells always maintain high autophagy levels. Emerging evidence ... regulation and functions of autophagy-induced circRNAs in TNBC remain unclear. In our study, autophagy-responsive circRNA candidates in TNBC cells under amino acid starved were identified by ... autophagic cells. Knockdown of circEGFR inhibited autophagy in TNBC cells, and circEGFR derived from exosomes induced autophagy ...
Tópico(s): Cancer-related molecular mechanisms research
2024 - Springer Nature | Oncogene
Long Lin, Xiangyu Fei, Liucui Chen, Yao Liang, Xiaoyong Lei,
Triple-negative breast cancer (TNBC) poses a significant clinical challenge due to its propensity for metastasis and poor prognosis. TNBC evades the body's immune system recognition and ... numerous solid tumors, exhibits pronounced activation in specific TNBC subtypes. Consequently, targeting the JAK2/STAT3 signaling pathway ... as a promising and precise therapeutic strategy for TNBC. The signal transduction cascade of the JAK2/STAT3 ... this pathway as a potential therapeutic target for TNBC treatment. This article comprehensively reviews preclinical and clinical ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2024 - Frontiers Media | Frontiers in Oncology
Weidan Zhang, Xin Tang, Yang Peng, Yingkun Xu, Liu Li, Shengchun Liu,
... major challenge in treating triple‑negative breast cancer (TNBC); chemotherapy remains the primary approach. The present study ... binding protein 2 (GBP2) in activating autophagy in TNBC and its impact on the sensitivity of TNBC cells to paclitaxel (PTX). Transfection with lentivirus was performed to establish TNBC cell lines with stable, high GBP2 expression. The ... quantitative PCR and western blotting, respectively. Autophagy in TNBC cells was evaluated using immunoblotting, transmission electron microscopy ...
Tópico(s): Epigenetics and DNA Methylation
2024 - Spandidos Publishing | International Journal of Oncology
Sanjib Chaudhary, Appadurai Muthamil Iniyan, Shailendra Kumar Maurya, Palanisamy Nallasamy, Saravanakumar Marimuthu, Ashu Shah, Pranita Atri, Chirravuri Venkata Ramakanth, Subodh M. Lele, Parthasarathy Seshacharyulu, Moorthy P. Ponnusamy, Mohd W. Nasser, Apar Kishor Ganti, Surinder K. Batra, Imayavaramban Lakshmanan,
Abstract Background Triple-negative breast cancer (TNBC) is highly aggressive with an increased metastatic incidence compared to other breast cancer subtypes. However, due to the absence of clinically reliable biomarkers and targeted therapy in TNBC, outcomes are suboptimal. Hence, there is an urgent ... to identifying novel therapeutic targets for managing metastatic TNBC. Methods The clinical significance of MUC16 and ELAVL1 ... Microarray was performed on MUC16 knockdown and scramble TNBC cells and MUC16-associated genes were identified using ...
Tópico(s): RNA and protein synthesis mechanisms
2023 - BioMed Central | Breast Cancer Research
Xie Hu, Ping Yang, Songhao Chen, Gang Wei, Lijuan Yuan, Zhenyu Yang, Li Gong, Li He, Lin Yang, Shujia Peng, Yanming Dong, Xianli He, Guo‐Qiang Bao,
... in some patients with triple-negative breast cancer (TNBC). However, its potential impacts on clinical features and tumor biological characteristics in TNBC remain unclear. Methods We enrolled 251 consecutive TNBC patients retrospectively, including 157 HER2-low (HER2 low ) ... cell RNA sequencing (scRNA-seq) with another seven TNBC samples (HER2 neg vs. HER2 low , 4 vs. ... differences of tumor biological properties between the two TNBC phenotypes. The underlying molecular distinctions were also explored and then verified in the additional TNBC samples. Results Compared with HER2 neg TNBC, HER2 ...
Tópico(s): Single-cell and spatial transcriptomics
2023 - BioMed Central | Breast Cancer Research
Arianna Giacomini, Marta Turati, Elisabetta Grillo, Sara Rezzola, Gaia C. Ghedini, Ander Churruca Schuind, Eleonora Foglio, Federica Maccarinelli, Jessica Faletti, Serena Filiberti, Angela Chambery, Mariangela Valletta, Laura Melocchi, Stéphanie Gofflot, Barbara Chiavarina, Andrei Turtoï, Marco Presta, Roberto Ronca,
... depending on tumor context. Triple negative breast cancer (TNBC) represents the most aggressive histotype of breast cancer, ... alternative druggable targets is of great interest in TNBC.The expression of PTX3 in BC tumor samples ... tumor and stromal cells to PTX3 production in TNBC was assessed by analyzing single cell RNA sequencing data and RNAscope performed on TNBC tumor samples. In order to investigate the effects of PTX3 in TNBC, different cell lines were engineered to knock-down ( ... the molecular mechanism(s) exerted by PTX3 in TNBC.In silico and experimental data indicate that PTX3 ...
Tópico(s): Circular RNAs in diseases
2023 - BioMed Central | Experimental Hematology and Oncology
Wei Wang, Priyanka S. Rana, Vesna Markovic, Khalid Sossey‐Alaoui,
... the USA and worldwide. Triple negative breast cancer (TNBC), which lacks expression of hormone receptors (ER-α ... WAVE3 has been established as a promoter of TNBC development and metastatic progression. In this study, we ... WAVE3 promotes therapy-resistance and cancer stemness in TNBC, through the regulation of β-catenin stabilization. Methods ... study the WAVE3/β-catenin oncogenic signaling in TNBC. Tumor xenograft assays were used to study the role of WAVE3 in mediating chemotherapy resistance of TNBC tumors. Results Genetic inactivation of WAVE3 in combination ...
Tópico(s): Tumors and Oncological Cases
2023 - BioMed Central | Breast Cancer Research
Bingye Shi, Wei Zhang, Tao Wang, Zhenyu Cui,
... related genes (CRGs) on triple negative breast cancer (TNBC) development, as well as their associated molecular mechanisms, ... Model, 11 CRGs may be especially important in TNBC development and progression (considered as the Key-TNBC-CRGs). The expression of several Key-TNBC-CRGs (e.g., ATP7A, PIK3CA, LIAS, and LIPT ) ... common mutations. The SCNA variation of 11 Key-TNBC-CRGs are related to differences immune infiltration profiles. ... 836). Finally, we discovered some potential drugs for TNBC treatment based on the strategy of targeting 11 ...
Tópico(s): RNA modifications and cancer
2023 - BioMed Central | BMC Bioinformatics
Meng Hao, Bin Huang, Renfei Wu, Zheng Peng, Kathy Qian Luo,
Triple-negative breast cancer (TNBC) has higher mortality than non-TNBC because of its stronger metastatic capacity. Increasing studies reported that TNBC tumors had more macrophage infiltration than non-TNBC tumors, which promoted the metastasis of TNBC cells. However, how TNBC cells become more malignant after interacting with macrophages ... In this study, it is observed that when TNBC cells are co-cultured with macrophages, they display higher viability and stronger metastatic ability than non-TNBC cells. Mechanistic studies reveal that TNBC cells acquired ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2023 - Wiley | Advanced Science
Synnøve Yndestad, Christina Engebrethsen, Andrea Herencia-Ropero, Oleksii Nikolaienko, Olav Karsten Vintermyr, Reidun Lillestøl, Laura Minsaas, Beryl Leirvaag, Gjertrud T. Iversen, Bjørnar Gilje, Egil Støre Blix, Helge Espelid, Steinar Lundgren, Jürgen Geisler, Hildegunn S. Aase, Turid Aas, Einar Gudlaugsson, Alba Llop‐Guevara, Violeta Serra, Emiel A. M. Janssen, Per Eystein Lønning, Stian Knappskog, Hans Petter Eikesdal,
... is highly prevalent in triple-negative breast cancer (TNBC) and associated with response to PARP inhibition (PARPi). ... we studied the prevalence of HRD in non-TNBC to assess the potential for PARPi in a ... HRD-S was present in 5% of non-TNBC cases (n = 9 of 169), contrasting 47% of the TNBC tumors (n = 15 of 32). HRD-W was observed in 23% of non-TNBC (n = 39 of 169) and 59% of TNBC cases (n = 19 of 32). Of 58 non-TNBC and 30 TNBC biopsies examined for RAD51 foci, 4 of 4 (100%) non-TNBC and 13 of 14 (93%) TNBC cases classified ...
Tópico(s): BRCA gene mutations in cancer
2023 - Lippincott Williams & Wilkins | JCO Precision Oncology
Weina Li, Guohui Han, Feng Li, Peng Bu, Yating Hao, Li Huang, Xiangdong Bai,
... and therapy resistance of triple‐negative breast cancer (TNBC). Currently, immune checkpoint inhibitors that regulate T‐cell ... L1, are emerging as new promising therapy for TNBC patients. However, only very limited patients showed complete ... the key reasons for the immune escape of TNBC. The regulation of exosome‐derived cirmiRNAs on CD8 + T cells in TNBC deserves more investigation. Here, the cirmiR‐20a‐5p level was significantly upregulated in the plasma of TNBC patients and culture supernatant of TNBC cells. High ...
Tópico(s): Circular RNAs in diseases
2023 - Wiley | Cancer Science
Shuizhong Cen, Xiaojie Peng, Jianwen Deng, Haiyun Jin, Zhinan Deng, X. Lin, Di Zhu, Ming Jin, Yanwen Zhu, Pusheng Zhang, Yunfeng Luo, Hong-yan Huang,
Triple-negative breast cancer (TNBC) is characterized by fast growth, high metastasis, high invasion, and a lack of therapeutic targets. Mitosis and metastasis of TNBC cells are two important biological behaviors in TNBC malignant progression. It is well known that the ... AFAP1-AS1 is involved in the mitosis of TNBC cells remains unknown. In this study, we investigated ... 1 (PLK1) activation and participating in mitosis of TNBC cells. We detected the expression of AFAP1-AS1 in the TNBC patient cohort and primary cells by in situ ...
Tópico(s): RNA Research and Splicing
2023 - Cognizant Communication Corporation | Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics
Tahir Zaib, Ke Cheng, Tingdang Liu, Ruyi Mei, Qin Liu, Xiaoling Zhou, Lifang He, Hibba Rashid, Qingdong Xie, Hanif Khan, Yien Xu, Pingnan Sun, Jundong Wu,
Triple-negative breast cancer (TNBC) accounts for 15–20% of all breast cancer cases. Due to the lack of expression of well-known molecular targets [ ... a need for more alternative treatment approaches in TNBC. Chimeric antigen receptor (CAR)-T cell-based immunotherapy ... aimed to investigate the expression of CD22 in TNBC. Bioinformatic analysis was performed to evaluate the expression ... performed to characterize the expression of CD22 in TNBC cell lines. Immunohistochemical (IHC) staining was performed on tumor specimens from 97 TNBC patients for CD22 expression. Moreover, statistical analysis was ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Clare M. Adams, Ramkrishna Mitra, You‐Cai Xiao, Peter Michener, Juan Palazzo, Allen Chao, Jitendra Gour, Joel Cassel, Joseph M. Salvino, Christine M. Eischen,
Triple-negative breast cancers (TNBC) frequently inactivate p53, increasing their aggressiveness and therapy resistance. We identified an unexpected protein vulnerability in p53-inactivated TNBC and designed a new PROteolysis TArgeting Chimera (PROTAC) ... VHL recruitment. MDM2 loss in p53 mutant/deleted TNBC cells in two-dimensional/three-dimensional culture and TNBC patient explants, including relapsed tumors, causes apoptosis while ... PROTAC is stable in vivo, and treatment of TNBC xenograft-bearing mice demonstrates tumor on-target efficacy ...
Tópico(s): Cancer-related Molecular Pathways
2023 - American Association for Cancer Research | Cancer Discovery
Jiaxin Zhang, Kaicheng Zhou, Jingbo Lin, Xianxian Yao, Dianwen Ju, Xian Zeng, Zhiqing Pang, Wuli Yang,
Triple-negative breast cancer (TNBC) causes great suffering to patients because of its heterogeneity, poor prognosis, and chemotherapy resistance. Ferroptosis is characterized by iron-dependent ... plays a vital role in the treatment of TNBC based on its intrinsic characteristics. To identify the relationship between chemotherapy resistance and ferroptosis in TNBC, we analyzed the single cell RNA-sequencing public ... that the expression of Gpx4 in DOX-resistant TNBC cells was significantly higher than that in DOX- ...
Tópico(s): RNA modifications and cancer
2023 - Elsevier BV | Biomaterials
Zhitao Han, Qi Jia, Jilei Zhang, Miaomiao Chen, Lining Wang, Kai Tong, Weiwei He, Yajie Zhang, Weina Zhu, Qin Ju, Tao Wang, Tielong Liu, Yong Ma, Yuanming Chen, Siluo Zha, Chunlei Zhang,
... initiation and progression of Triple-negative breast cancer (TNBC). The publicly available TCGA database of breast cancer ... to assess YOD1 expression and function in human TNBC and then explored the underlying molecular events. Methods We detected the expression of YOD1 in 32 TNBC and 44 NTNBC samples by qRT-PCR, Western ... was assessed in vitro and in vivo for TNBC cell proliferation, migration, invasion, cell-cycle and drug ... was found that YOD1 was significantly upregulated in TNBC tissues compared with non-triple-negative breast cancer ( ...
Tópico(s): Metal-Catalyzed Oxygenation Mechanisms
2023 - BioMed Central | Journal of Experimental & Clinical Cancer Research
Lifang Yuan, Jiannan Liu, Lei Bao, Huajun Qu, Jinyu Xiang, Ping Sun,
... regulator gene (FRG) expression patterns in patients with TNBC based on data derived from The Cancer Genome ... TCGA). Further, it was utilized to establish a TNBC FRG signature, after which the association between this ... RNA expression datasets and clinical information about 190 TNBC patients, after which a prognostic TNBC-related FRG signature was established using a least ... data from the Gene Expression Omnibus (GEO). The TNBC-specific prognostic gene was identified via this method. ... RT-qPCR) analyses of clinical tissue samples and TNBC cell lines. Chemotherapy interactions and predicted drug sensitivity ...
Tópico(s): RNA modifications and cancer
2023 - Frontiers Media | Frontiers in Oncology
Huibian Zhang, Min Gao, Wenying Zhao, Lin Yu,
... of breast cancer cells. Triple-negative breast cancer (TNBC) is a specific breast cancer subtype with high ... the specific roles and mechanisms of SND1 in TNBC metastasis remain unaddressed.Immunostaining was used to detect the SND1 expression in tissue samples of 58 TNBC and 10 glioblastomas (GBM) as positive control. The ... assays to investigate the function of SND1 in TNBC cell migration and invasion.The data of immunohistochemistry manifested that SND1 is the overexpression in metastasized TNBC and an independent factor for TNBC prognosis. SND1 ...
Tópico(s): Chromatin Remodeling and Cancer
2023 - BioMed Central | Breast Cancer Research
Li-Feng Dong, Fangfang Chen, Yang-Fan Fan, Kun Zhang, Huihui Chen,
... in the progression of triple-negative breast cancer (TNBC). Methods circ-0000512 expression in TNBC tissues and paired adjacent normal tissues and cells ... by qRT-PCR. Moreover, circ-0000512 expression in TNBC cells was modulated by transfection. Thereafter, colony formation ... conducted to observe cell proliferation, migration and apoptosis. TNBC cells were treated with cycloheximide and the protease ... cell death ligand 1 (PD-L1) ubiquitination in TNBC cells. The T cell killing ability was assessed ... Results circ-0000512 was upregulated in patients with TNBC. circ-0000512 knockdown attenuated the proliferation and migration ...
Tópico(s): MicroRNA in disease regulation
2023 - BMJ | Journal for ImmunoTherapy of Cancer
Chinnadurai Mani, Ganesh Acharya, Karunakar Saamarthy, Damieanus Ochola, Srinidhi Mereddy, Kevin Pruitt, Upender Manne, Komaraiah Palle,
Abstract Background Triple-negative breast cancer (TNBC) affects young women and is the most aggressive subtype of breast cancer (BC). TNBCs disproportionally affect women of African-American (AA) descent ... gene RAD51 as a poor prognosis marker in TNBC and its posttranscriptional regulation through microRNAs (miRNAs). This ... and develop novel therapeutic combinations to effectively treat TNBCs and reduce disparity in clinical outcomes. Methods Analysis ... and PrognoScan identified the overexpression of RAD51 in TNBCs. miRNA sequencing identified significant downregulation of RAD51-targeting ...
Tópico(s): Genomics and Chromatin Dynamics
2023 - BioMed Central | Breast Cancer Research
Kaushik Das, Subhojit Paul, Arnab Ghosh, Saurabh Gupta, Tanmoy Mukherjee, Prem Shankar, Anshul Sharma, Shiva Keshava, Subhash C. Chauhan, Vivek K. Kashyap, Deepak Parashar,
Triple-negative breast cancer (TNBC) is an aggressive subtype accounting for ~10-20% of all human BC and is characterized by the absence of estrogen ... its unique molecular profile and limited targeted therapies, TNBC treatment poses significant challenges. Unlike other BC subtypes, TNBC lacks specific molecular targets, rendering endocrine therapies and ... regimen is the predominant systemic treatment modality for TNBC in current clinical practice. However, the efficacy of chemotherapy in TNBC is variable, with response rates varying between a ...
Tópico(s): Nanoplatforms for cancer theranostics
2023 - Multidisciplinary Digital Publishing Institute | Cancers
Terrance J. Lynn, Jianhui Shi, Haiyan Liu, Sara E. Monaco, Jeffrey W. Prichard, Fan Lin,
... Definitive diagnosis of metastatic triple-negative breast carcinoma (TNBC) is challenging on cytologic samples. Recent studies demonstrated ... and specific marker for diagnosing breast carcinomas, including TNBC, on surgical specimens. Objective.— To evaluate TRPS1 expression in TNBCs on cytologic samples and a large series of ... binding protein 3 (GATA3) was performed on 35 TNBC cases on surgical specimens, and 29 consecutive TNBC cases on cytologic specimens. IHC analysis of TRPS1 ... Results.— Of the surgical specimens, 35 of 35 TNBC cases (100%) were positive for TRPS1, all with ...
Tópico(s): Cancer Genomics and Diagnostics
2023 - American Medical Association | Archives of Pathology & Laboratory Medicine
Shuning Ding, Q. L. Niu, Qingchen Zhu, Yiwei Tong, Shengyue Wang, Xiaosong Chen, Qiang Tian, Yichuan Xiao, Kunwei Shen,
... the immune microenvironment of triple negative breast cancer (TNBC) compared with other subtypes of breast cancer remain ... to depict and compare the immune landscape among TNBC, human epidermal growth factor receptor 2‐positive (HER2 + ) ... as well as transcriptome features were compared among TNBC, human HER2 + breast cancer, and luminal‐like breast ... clusters were identified. A unique immunosuppressive microenvironment in TNBC was decoded as compared to that in HER2 + ... cells. Tregs and exhausted CD8 + T cells in TNBC exhibited increased immunosuppression signature and dysfunctional scores. Pseudotime ...
Tópico(s): T-cell and B-cell Immunology
2023 - Wiley | Cancer Communications
Ling Deng, Li Liao, Yinling Zhang, Shu‐Yuan Hu, Shao‐Ying Yang, Xiao-Yan Ma, Min-Ying Huang, Fang-Lin Zhang, Da‐Qiang Li,
Triple-negative breast cancer (TNBC), although highly lethal, lacks validated therapeutic targets. Here, we report that U2 snRNP-associated SURP motif-containing protein (U2SURP), a ... arginine rich protein family, was significantly upregulated in TNBC tissues, and its high expression was associated with poor prognosis of TNBC patients. MYC, a frequently amplified oncogene in TNBC tissues, enhanced U2SURP translation through an eIF3D (eukaryotic ... mechanism, resulting in the accumulation of U2SURP in TNBC tissues. Functional assays revealed that U2SURP played an ...
Tópico(s): Cancer-related gene regulation
2023 - Elsevier BV | Cancer Letters