Shinya Suzu, Nobuya Yanai, Minoru Saito, Takuji Kawashima, Tadashi Kasahara, Masaki Saito, Fumimaro Takaku, Kazuo Motoyoshi,
ABSTRACT Human monocytic colony‐stimulating factor (hM‐CSF) stimulates mature cells as well as progenitor cells of human monocyte lineage. In the present study, we investigated the effect of completely purified native hM‐CSF on the tumoricidal activity of human monocytes against Raji (Burkitt lymphoma‐derived cell line) using a chromium release assay. Preincubation of human peripheral blood monocytes with hM‐CSF for 48 h markedly enhanced their antibody‐dependent cellular cytotoxicity (ADCC). This ...
Tópico(s): Immunotherapy and Immune Responses
1990 - Oxford University Press | Japanese Journal of Cancer Research
Hideki Ozawa, Masaharu Kotani, Ikuo Kawashima, Tadashi Tai,
We established six murine monoclonal antibodies (MAbs) specific for b-pathway ganglio-series gangliosides by immunizing C3H/HeN mice with these purified gangliosides adsorbed to Salmonella minnesota mutant R595. The binding specificities of these MAbs were determined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatogram. These six MAbs, designated GGB19, GMR2, GMR7, GGR12, GMR5, and GGR13 reacted strongly with the gangliosides GD3, O-Ac-GD3, GD2, GD1b, GT1b, and GQ1b, ...
Tópico(s): Galectins and Cancer Biology
1992 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Hideki Ozawa, Ikuo Kawashima, Tadashi Tai,
Tópico(s): Galectins and Cancer Biology
1992 - Elsevier BV | Archives of Biochemistry and Biophysics
Kazuya I.P.J. Hidari, Ikuo Kawashima, Tadashi Tai, Fuyuhiko Inagaki, Yoshitaka Nagai, Yutaka Sanai,
Two gangliosides were efficiently synthesized from asialo‐G m1 (Galβ1‐3GalNAcβ1‐4Galβ1‐4Glcβ1‐1 Cer) and cytidine 5′‐phosphate‐ N ‐acetylneuraminic acid (CMP‐NeuAc) by using sialyltransferases in rat liver Golgi vesicles in vitro . These gangliosides were rapidly purified by a combination of anion exchange and reverse‐phase column chromatographies. The ganglioside structures were determined by TLC analysis, treatment with a sialidase from Salmonella typhimurium LT2, which specifically hydrolyzes α2‐3 ...
Tópico(s): Caveolin-1 and cellular processes
1994 - Wiley | European Journal of Biochemistry
Takuma Matsuoka, Takuji Kawashima, Tadashi Nakamura, Yoshihiro Kanamaru, Tomio Yabe,
Tópico(s): Insect and Arachnid Ecology and Behavior
2012 - Springer Science+Business Media | Apidologie
Susumu Kusunoki, Atsuro Chiba, Yoshio Hirabayashi, Fumitoshi Irie, Masaharu Kotani, Ikuo Kawashima, Tadashi Tai, Yoshitaka Nagai,
We have established a monoclonal antibody, GGR41, specific for a new class of minor gangliosides, such as GQ1b alpha and GT1a alpha, by immunizing mice with a GQ1b-rich ganglioside fraction extracted from bovine brain. Each of those minor gangliosides has been reported to be one of the cholinergic-specific gangliosides (Chol-1). Careful examination of binding specificity of the antibody by both an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms showed that the antibody ...
Tópico(s): Neuroblastoma Research and Treatments
1993 - Elsevier BV | Brain Research
Kiyomitsu Nara, Yumiko Watanabe, Ikuo Kawashima, Tadashi Tai, Yoshitaka Nagai, Yutaka Sanai,
To address the role of α2,8‐sialyltransferase (G D3 synthase) in the biosynthesis of gangliosides, we examined the substrate specificity of the enzyme. In the ganglioside synthesis pathway, it has been generally accepted that sialyltransferase II (SAT II) catalyzes the production of G D3 from G M3 , and sialyltransferase V (SAT V) catalyzes the production of G D1c /G T1a /G Q1b from G M1b /G D1a /G T1b . However, acceptor specificity of the cloned G D3 synthase that was isolated from human melanoma cells [Nara, ...
Tópico(s): Enzyme Structure and Function
1996 - Wiley | European Journal of Biochemistry
Yuri Kashima, Satoshi Kanematsu, Saori Asai, Mio Kusada, Suzuyo Watanabe, Takuji Kawashima, Tadashi Nakamura, Masaya Shimada, Tsuyoshi Goto, Satoshi Nagaoka,
Royal jelly (RJ) intake lowers serum cholesterol levels in animals and humans, but the active component in RJ that lowers serum cholesterol level and its molecular mechanism are unclear. In this study, we set out to identify the bile acid-binding protein contained in RJ, because dietary bile acid-binding proteins including soybean protein and its peptide are effective in ameliorating hypercholesterolemia. Using a cholic acid-conjugated column, we separated some bile acid-binding proteins from RJ ...
Tópico(s): Phytoestrogen effects and research
2014 - Public Library of Science | PLoS ONE
Tetsuya Takahashi, Mitsuo Kawashima, Tadashi Yasuoka, Michiharu Kamiyoshi, Katuhide TANAKA,
Mesotocin and arginine vasotocin were injected intravenously once at various dosage levels (.0025 to 25 μg/kg body weight) immediately after an intravenous injection of 15 mL/kg body weight saline solution into hens in which an artificial anus had been surgically created, and the volume of urine excreted during a 5-h period, following the injection, was measured. Arginine vasotocin caused a monophasic, dose-dependent decrease in urine volume. By contrast, mesotocin was biphasic: at .025 and .25 μg/kg ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
1995 - Elsevier BV | Poultry Science
Yasuhisa Kuroda, Ayato Kawashima, Tadashi Urai, Hisanobu Ogoshi,
Atropisomerization of meso-tetrakis(2-carboxy-4-nonylphenyl)porphyrin 1, in nonpolar solvents such as CHCl3 gives the αααα isomer exclusively, while the same isomerization in polar solvents such as DMSO proceeds normally. Spectroscopic investigations of 1 suggest that the porphyrin exists as a cofacial dimer in nonpolar solvents, where eight hydrogen bonds among tour pairs of carboxylic acids in 1 are formed.
Tópico(s): Molecular spectroscopy and chirality
1995 - Elsevier BV | Tetrahedron Letters
Jun Fujishiro, Tetsuya Ishimaru, Masahiko Sugiyama, Mari Arai, Keisuke Suzuki, Hiroshi Kawashima, Tadashi Iwanaka,
Tópico(s): Congenital Anomalies and Fetal Surgery
2015 - Springer Science+Business Media | Surgery Today
Shizuya Saika, Akifumi Yamanaka, Akio Yamanaka, Ayako Minamide, Keisyu Kin, Kumi Shirai, Sai‐ichi Tanaka, Yoshiji Kawashima, Tadashi Katoh, Yuka Okada, Kiyomi Ohkawa, Yoshitaka Ohnishi,
Tópico(s): Retinal Diseases and Treatments
1998 - Springer Science+Business Media | Graefe s Archive for Clinical and Experimental Ophthalmology
Nobuyuki Kawashima, Tadashi Yagi, Kouya Kojima,
Because plastics contribute to healthy and sound everyday life, global usage volume of plastic is expected to increase. However, the chemical industry is facing challenges in plastics from the viewpoints of resource depletion and environmental burden. These trends have led to discussions on how plastics should move forward in a sustainable society and a circular economy considering resource conversion, efficient after‐use utilization, and environmental protection. Bioplastics, both bio‐based and ...
Tópico(s): Chemistry and Chemical Engineering
2019 - Wiley | Macromolecular Materials and Engineering
Reiko Saito, Hidekazu Osada, Keita Wagatsuma, Irina Chon, Isamu Sato, Takashi Kawashima, Tadashi Saito, Naoki Kodo, Yasuhiko Ono, Yasushi Shimada, WintWint Phyu, Yugo Shobugawa,
We conducted a prospective, multicenter, non-randomized observational study to assess the duration of fever and symptoms of influenza A/H1N1pdm09 and A/H3N2 infected children < 19 years old treated with either baloxavir or oseltamivir. Additionally, these symptoms were investigated in association with pre- and post-baloxavir treatment-emergent polymerase acidic unit (PA) variants as compared to non-substituted viruses. Following receipt of informed consent, baloxavir was administered to 102 influenza ...
Tópico(s): Viral Infections and Outbreaks Research
2020 - Elsevier BV | Antiviral Research
Nobuyuki Kawashima, Tadashi Yagi, Kouya Kojima,
The chemical industry and subsequent value chain of plastics are facing significant challenges from the viewpoints of resource conversion and environmental burden. Now is the time to explore the future direction of plastics, which will require an integrated scheme using resource circulation, carbon neutrality, and a social system to promote after-use treatment under the concept of a circular economy. Polylactic acid (PLA) should help reduce greenhouse gas (GHG) emissions as a biobased material and ...
Tópico(s): Sustainable Supply Chain Management
2021 - Multidisciplinary Digital Publishing Institute | Sustainability
Yoshikazu Tanaka, Toru Hayashi, Koji Kawashima, Tadashi Yokoyama, Toshio Watanabe,
By the radiation-copolymerization method with polyethylene glycoldimethacrylate (PGD) as a main polymerizable reagent, microbial cells of Brevibacterium ammoniagenes were immobilized with high specific activity of NAD kinase and high mechanical strength. The reagents used for the immobilization such as PGD, polyvinyl alcohol (PVA), and N,N'-methylenebisacrylamide (Bis) did not inversely affect the enzyme activity. Freezing and irradiation treatment of the cell-reagent solution did not inactivate the ...
Tópico(s): Pancreatic function and diabetes
1982 - Wiley | Biotechnology and Bioengineering
Masaharu Horii, Takashi Ide, Kenji KAWASHIMA, Tadashi Yamamoto,
Effect of dietary miso on the cholesterol metabolism was examined in rats fed the diet containing 0.5% cholesterol and 0.15% sodium cholate. The inclusion of 20% desalted miso to the atherogenic diet significantly decreased the concentration of serum cholesterol throughout the 30 days experimental period. Serum triglyceride and phospholipid as well as hepatic lipid concentrations at the time of sacrifice (30 days) were in no way affected by dietary miso. The ingestion of the miso preparation doubled ...
Tópico(s): Natural Antidiabetic Agents Studies
1990 - | NIPPON SHOKUHIN KOGYO GAKKAISHI
Hiroshi Kawashima, Tadashi Iwanaka, Masatomo Matsumoto, Hajime Takayasu, Toshinori Aihara, Hiroshi Kishimoto, Satohiko Imaizumi,
Pyloric stenosis caused by gastrointestinal duplication is an extremely rare condition in neonates and young infants; only a few cases have been reported (1-4). Histologic evaluation revealed gastric duplication in all cases except one, which involved a pyloroduodenal duplication (5). Pyloric stenosis in all of the cases was caused by cystic formation of gastroduodenal duplication with no exceptions. In the case we report, an upper gastrointestinal barium study (UGI) accurately indicated pyloric stenosis ...
Tópico(s): Gastrointestinal disorders and treatments
1998 - Lippincott Williams & Wilkins | Journal of Pediatric Gastroenterology and Nutrition
Takayuki Kawashima, Tadashi Umeno, Takeshi Terazawa, Tomoyuki Wada, Takashi Shuto, Haruto Nishida, Hirofumi Anai, Yasuhide Nakayama, Shinji Miyamoto,
Abstract OBJECTIVES Aortic valve neocuspidization has shown satisfactory clinical outcomes; however, autologous pericardium durability is a concern for young patients. This study applied an autologous collagenous membrane (Biosheet®), produced by in-body tissue architecture, to aortic valve neocuspidization and investigated its long-term outcome in a goat model. METHODS Moulds were embedded subcutaneously in 6 goats. After 2 months, Biosheets formed in the moulds. We performed aortic valve neocuspidization ...
Tópico(s): Aortic aneurysm repair treatments
2021 - Oxford University Press | Interactive Cardiovascular and Thoracic Surgery
Koji Takahashi, Kayoko Saito, Katsuhiko Tamura, Michiyo Honda, H Touei, Osamu Sakai, Y Kawashima, Tadashi Kuji, Masashi Ohtani, Terufumi Kawamoto,
A new technique for hepatic intraarterial digital subtraction angiography (DSA)--hepatoportal subtraction angiography (HPSA)--that emphasizes the enhancement of tumors by suppressing liver parenchymal enhancement has been developed. The tumor detection sensitivity of HPSA was evaluated prospectively in comparison with that of conventional hepatic intraarterial DSA, unenhanced computed tomography (CT) or CT enhanced with iodine-containing contrast material (conventional CT), CT with iodized oil, and ...
Tópico(s): Genetic and Kidney Cyst Diseases
1990 - Radiological Society of North America | Radiology
Tomohisa Sakaue, Tadaaki Koyama, Yoshitsugu Nakamura, Keitaro Okamoto, Takayuki Kawashima, Tadashi Umeno, Yasuhide Nakayama, Shinji Miyamoto, Fumiaki Shikata, Mika Hamaguchi, Jun Aono, Mie Kurata, Kenji Namiguchi, Shunji Uchita, Junya Masumoto, Osamu Yamaguchi, Shigeki Higashiyama, Hironori Izutani,
Histologic evaluations revealed excessive accumulations of macrophages and absence of fibroblastic interstitial cells in explanted bioprosthetic valves. Comprehensive gene and protein expression analysis and histology unveiled an accumulation of fibrinogen and plasminogen, an activator of infiltrated macrophages, from degenerated valve surfaces in the interstitial spaces. These pathologies were completely reproduced in a goat model replaced with an autologous pericardium-derived aortic valve. Further ...
Tópico(s): Orthopaedic implants and arthroplasty
2023 - Elsevier BV | JACC Basic to Translational Science
Takashi Matsuura, Hiroaki Uetake, Tadahiro Ohmi, Junichi Murota, Koichi Fukuda, Nobuo Mikoshiba, Tadashi Kawashima, Yoshihiro Yamashita,
Using a newly developed ultraclean electron cyclotron resonance plasma etcher, Si wafers masked by SiO2 were etched with a chlorine plasma at pressures of 0.6–4.0 mTorr with a microwave power of 300–700 W. Ultraclean processing under a low ion energy condition at high pressures has revealed that there is an induction period during which time there is no SiO2 etching. This is not observed with Si. During the induction period, perfectly selective etching for Si to SiO2 has been achieved. Under this ...
Tópico(s): Metal and Thin Film Mechanics
1990 - American Institute of Physics | Applied Physics Letters
Tadashi Kawashima, Matteo Pellegrini, Guillaume Chanfreau,
The role of many splicing factors in pre-mRNA splicing and the involvement of these factors in the processing of specific transcripts have often been defined through the analysis of loss-of-function mutants in vivo. Here we show that inactivating the nonsense-mediated mRNA decay (NMD) results in an enhancement of splicing phenotypes associated with several S. cerevisiae splicing factor mutations. Tiling microarrays showed that inactivation of the NMD factor Upf1p in the prp17Delta and prp18Delta ...
Tópico(s): RNA modifications and cancer
2009 - Cold Spring Harbor Laboratory Press | RNA
Tsuyoshi Satoh, Tadashi Kawashima, Satoru Takahashi, Ken Sakai,
Tópico(s): Chemical Synthesis and Reactions
2003 - Elsevier BV | Tetrahedron
Tsunenori Matsunaga, Masaki Tsujimoto, Tadashi Kawashima, Yasuhisa Tsujimoto, Mamoru Fujiwara, Atsushi Ookubo, Yoshihiko Hayashi,
The aim of the present study was to investigate whether the concentration of arsenic (As) released from gray or white mineral trioxide aggregates (MTAs) met the requirement of the International Standards Organization (ISO) for dental cements.Sample preparations were carried out according to the ISO methods. After centrifugation of dissolved samples, As (III) concentration in the final supernatant was analyzed by a high-performance atomic absorption spectrophotometer.As (III) concentration from both MTAs ...
Tópico(s): Sinusitis and nasal conditions
2010 - Elsevier BV | Journal of Endodontics
Defne E. Egecioglu, Tadashi Kawashima, Guillaume Chanfreau,
The MAT a1 gene encodes a transcriptional repressor that is an important modulator of sex-specific gene expression in Saccharomyces cerevisiae . MAT a1 contains two small introns, both of which need to be accurately excised for proper expression of a functional MAT a1 product and to avoid production of aberrant forms of the repressor. Here, we show that unspliced and partially spliced forms of the MAT a1 mRNA are degraded by the nuclear exonuclease Rat1p, the nuclear exosome and by the nuclear RNase ...
Tópico(s): RNA Interference and Gene Delivery
2011 - Oxford University Press | Nucleic Acids Research
Christopher M. Allan, Shauna Hill, Susan F. Morvaridi, Ryoichi Saiki, Jarrett Johnson, Wei‐Siang Liau, Kathleen Hirano, Tadashi Kawashima, Ziming Ji, Joseph A. Loo, Jennifer N. Shepherd, Catherine F. Clarke,
Coenzyme Qn (ubiquinone or Qn) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail of n isoprene units. Saccharomyces cerevisiae coq1–coq9 mutants have defects in Q biosynthesis, lack Q6, are respiratory defective, and sensitive to stress imposed by polyunsaturated fatty acids. The hallmark phenotype of the Q-less yeast coq mutants is that respiration in isolated mitochondria can be rescued by the addition of Q2, a soluble Q analog. Yeast coq10 mutants ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2012 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Tadashi Kawashima, Stephen M. Douglass, Jason Gabunilas, Matteo Pellegrini, Guillaume Chanfreau,
Saccharomyces cerevisiae has been used as a model system to investigate the mechanisms of pre-mRNA splicing but only a few examples of alternative splice site usage have been described in this organism. Using RNA-Seq analysis of nonsense-mediated mRNA decay (NMD) mutant strains, we show that many S. cerevisiae intron-containing genes exhibit usage of alternative splice sites, but many transcripts generated by splicing at these sites are non-functional because they introduce premature termination ...
Tópico(s): RNA modifications and cancer
2014 - Public Library of Science | PLoS Genetics
Tomohiko ISE, Naoyuki Arita, Toshihiko Asami, Takenori Nakajima, Iwao Kawashima, Tadashi Maeda,
We have developed externally pressurized small-size conical shaped gas bearings for micro rotary machines such as gas blowers, compressors, and medical devices. The bearings are lightweight and enable machines and devices to be downsized by reducing the number of parts, since the journal and thrust loads can be supported by a pair of bearings. The conical bearings (diameters 8 mm and 10 mm) were designed and the manufacturing techniques were considered. The bearing type is a double-row inherent orifice ...
Tópico(s): Gear and Bearing Dynamics Analysis
2014 - Elsevier BV | Precision Engineering
Hideo Kubo, Masaharu Kotani, Hideki Ozawa, Lkuo Kawashima, Tadashi Tai, Akemi Suzuki,
A frozen section technique for frog oocytes was developed without using any organic solvent. It was applied to examine the distribution of acidic glycosphingolipids (ganglioside GM1 and sulfatide) in Xenopus oocytes, eggs and embryos by indirect immunofluorescence microscopy with specific monoclonal antibodies against the acidic glycolipids. Although glycolipids are generally present on the cell surface, GM1 and sulfatide were distributed in the cytoplasm of animal and vegetal hemispheres, respectively, ...
Tópico(s): Neurobiology and Insect Physiology Research
1995 - Wiley | Development Growth & Differentiation