Xinshan Zang, Xiaoli Geng, Fei Wang, Zhenshan Liu, Liyuan Zhang, Yue Zhao, Xuejun Tian, Zhongfu Ni, Yingyin Yao, Mingming Xin, Zhaorong Hu, Qixin Sun, Huiru Peng,
... thermotolerance are largely unknown. A novel ferritin gene, TaFER, was identified from our previous heat stress-responsive ... analysis of a heat-tolerant wheat cultivar (TAM107). TaFER was mapped to chromosome 5B and named TaFER-5B. Expression pattern analysis revealed that TaFER-5B was induced by heat, polyethylene glycol (PEG), ... acid (Fe-EDDHA). To confirm the function of TaFER-5B in wheat, TaFER-5B was transformed into the wheat cultivar Jimai5265 ( ... ferritin from mono- and dico-species is conserved, TaFER-5B was transformed into Arabidopsis, and overexpression of ...
Tópico(s): Plant responses to water stress
2017 - BioMed Central | BMC Plant Biology
Huadong Zhang, Jinghan Song, Feiyan Dong, Yaqian Li, Shijie Ge, Bo Wei, Yike Liu,
... acting elements and expression patterns analysis suggested that TaFERs might play an important role combating to abiotic ... current study provides insight into the role of TaFER genes in wheat.
Tópico(s): Legume Nitrogen Fixing Symbiosis
2023 - Elsevier BV | Plant Physiology and Biochemistry
Yuji Omata, Tomoaki Yamauchi, Akito Tsuruta, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo,
... R. Bernhart S.H. Höner zu Siederdissen C. Tafer H. Flamm C. Stadler P.F. Hofacker I. ...
Tópico(s): RNA Interference and Gene Delivery
2021 - Elsevier BV | Journal of Biological Chemistry
Tobias Diehl, John Clinton, Carlo Cauzzi, Toni Kraft, Philipp Kästli, N. Deichmann, Frédérick Massin, Francesco Grigoli, Irene Molinari, Maren Böse, Manuel Hobiger, Florian Haslinger, Donat Fäh, Stefan Wiemer,
... by an M L 2.9 earthquake near Tafers in 2018. The event images a NW–SE ...
Tópico(s): Landslides and related hazards
2021 - Springer Nature | Swiss Journal of Geosciences
Matteo Di Nardo, Aparna Hoskote, Tim Thiruchelvam, Jon Lillie, Marie Horan, Sylvia Belda Hofheinz, Laurent Dupic, R. Gimeno, Maria Elena De Piero, Valeria Lo Coco, Peter P. Roeleveld, Marc D. Davidson, Tim Jones, Lars Mikael Broman, Roberto Lorusso, Jan Bělohlávek,
... Padova, Padova, Italy); Olivier Brissaud, MD and Nadir Tafer, MD (SAR Sud Hopital Haut Léveque, CHU Bordeaux, ...
Tópico(s): Cardiac Structural Anomalies and Repair
2020 - Lippincott Williams & Wilkins | ASAIO Journal
Meiling Zhang, Hui Wang, Ting Nie, Jintao Bai, Fei Zhao, Shenghua Ma,
... GO were significantly improved. Furthermore, electrochemical impedance and Tafer polarisation curves showed that added 0.5 wt% ...
Tópico(s): High voltage insulation and dielectric phenomena
2020 - De Gruyter | Corrosion Reviews
Guadalupe Piñar, Hakim Tafer, Manfred Schreiner, Heinz Miklas, Katja Sterflinger,
Summary This study provides an example in the emerging field of biocodicology showing how metagenomics can help answer relevant questions that may contribute to a better understanding of the history of ancient manuscripts. To this end, two Slavonic codices dating from the 11th century were investigated through shotgun metagenomics. Endogenous DNA enabled to infer the animal origin of the skins used in the manufacture of the two codices, while nucleic sequences recovered from viruses were investigated ...
Tópico(s): Building materials and conservation
2020 - Wiley | Environmental Microbiology
Chao Shu, Hao Du, Wenhong Pu, Changzhu Yang, Jianyu Gong,
... 43V at 10 mA cm−2 with a Tafer slope of 63 mV dec−1, which were ...
Tópico(s): Electrochemical Analysis and Applications
2020 - Elsevier BV | International Journal of Hydrogen Energy
Guadalupe Piñar, Caroline Poyntner, Ksenija Lopandić, Hakim Tafer, Katja Sterflinger,
Living organisms leave traces of DNA in the objects they colonize and contribute to the microbiome of a given environment. The microbiome of cultural objects is an indicator of their conservation status and restoration history. Two oil paintings on canvas from the 18th and 19th centuries, one of them (painting M) showing an active fungal colonization, while the second (painting N) with a cracked varnish surface, were investigated by metagenomic analyses using whole genome amplification (WGA) and the ...
Tópico(s): Cultural Heritage Materials Analysis
2020 - Elsevier BV | International Biodeterioration & Biodegradation
Hakim Tafer, Caroline Poyntner, Ksenija Lopandić, Katja Sterflinger, Guadalupe Piñar,
Salt mines are among the most extreme environments as they combine darkness, low nutrient availability, and hypersaline conditions. Based on comparative genomics and transcriptomics, we describe in this work the adaptive strategies of the true halophilic fungus
Tópico(s): Microbial Natural Products and Biosynthesis
2019 - Multidisciplinary Digital Publishing Institute | Genes
Guadalupe Piñar, Caroline Poyntner, Hakim Tafer, Katja Sterflinger,
In this study, three possibly smuggled marble statues of an unknown origin, two human torsi (a female and a male) and a small head, were subjected to molecular analyses. The aim was to reconstruct the history of the storage of each single statue, to infer the possible relationship among them, and to elucidate their geographical shift. A genetic strategy, comprising metagenomic analyses of the 16S ribosomal DNA (rDNA) of prokaryotes, 18S rDNA of eukaryotes, as well as internal transcribed spacer regions ...
Tópico(s): Environmental DNA in Biodiversity Studies
2019 - BioMed Central | Annals of Microbiology
Katja Sterflinger, Christian Voitl, Ksenija Lopandić, Guadalupe Piñar, Hakim Tafer,
Recent investigations have shown that xerophilic fungi may pose a biodeterioration risk by threatening objects of cultural heritage including many types of materials, including wood, paint layers, organic glues or leather and even metal. Historic—and also new built—pipe organs combine all those materials. In this study, halotolerant aspergilli and penicillia with low optimal temperatures were shown to be the most frequent invaders of pipe organs. The fungi form white mycelia on the organic components ...
Tópico(s): Building materials and conservation
2018 - Multidisciplinary Digital Publishing Institute | Life
Caroline Poyntner, Ursula Mirastschijski, Katja Sterflinger, Hakim Tafer,
The black yeast Exophiala dermatitidis is a polyextremophilic human pathogen, especially known for growing in man-made extreme environments. Reported diseases caused by this fungus range from benign cutaneous to systemic infections with 40% fatality rate. While the number of cases steadily increases in both immunocompromised and immunocompetent people, detailed knowledge about infection mechanisms, virulence factors and host response are scarce. To understand the impact of the putative virulence ...
Tópico(s): Nail Diseases and Treatments
2018 - Frontiers Media | Frontiers in Microbiology
Jan Hoinka, Rolf Backofen, Teresa M. Przytycka,
... R. Bernhart S.H. Höner Zu Siederdissen C. Tafer H. Flamm C. Stadler P.F. Hofacker I. ...
Tópico(s): DNA and Nucleic Acid Chemistry
2018 - Cell Press | Molecular Therapy — Nucleic Acids
María Garnica, Eva Bacaicoa, Verónica Mora, Sara San Francisco, Roberto Baigorri, Ángel M. Zamarreño, José María García‐Mina,
... genes encoding the nicotianamine aminotransferase (TaNAAT) and ferritin (TaFER) have been evaluated in plants subjected to different ...
Tópico(s): Plant nutrient uptake and metabolism
2018 - BioMed Central | BMC Plant Biology
Donatella Tesei, Hakim Tafer, Caroline Poyntner, Guadalupe Piñar, Ksenija Lopandić, Katja Sterflinger,
The fungal genus Knufia mostly comprises extremotolerant species from environmental sources, especially rock surfaces. The draft genome sequence of the rock fungus Knufia petricola presented here is the first whole-genome sequence of the only species among black fungi known to have a nonmelanized spontaneous mutant.
Tópico(s): Infectious Diseases and Mycology
2017 - American Society for Microbiology | Genome Announcements
Barbara Blasi, Hakim Tafer, Christina Kustor, Caroline Poyntner, Ksenija Lopandić, Katja Sterflinger,
Abstract Cladophialophora immunda is an ascomycotal species belonging to the group of the black yeasts. These fungi have a thick and melanized cell wall and other physiological adaptations that allows them to cope with several extreme physical and chemical conditions. Member of the group can colonize some of the most extremophilic environments on Earth. Cladophialophora immunda together with a few other species of the order Chaetothyriales show a special association with hydrocarbon polluted environments. ...
Tópico(s): Genomics and Phylogenetic Studies
2017 - Nature Portfolio | Scientific Reports
Justyna Adamiak, Anna Otlewska, Hakim Tafer, Ksenija Lopandić, Beata Gutarowska, Katja Sterflinger, Guadalupe Piñar,
The aim of this study was to detect the community structure of Bacteria and Archaea, especially halophilic microorganisms, involved in the biodeterioration of historic building materials, as brick and paint coating. The Ion Torrent™ sequencing technology platform was used for the first time to analyse these materials. Amplicons were generated by using universal, bacteria- and archaea-specific primers, all flanking the V4 hypervariable region of the 16S rDNA, in order to compare the sensitivity of ...
Tópico(s): Cultural Heritage Materials Analysis
2017 - Elsevier BV | International Biodeterioration & Biodegradation
Marcel Zámocký, Hakim Tafer, Katarína Chovanová, Ksenija Lopandić, Anna Kamlárová, Christian Obinger,
The ascomycetous family Chaetomiaceae (class Sordariomycetes) includes numerous soilborn, saprophytic, endophytic and pathogenic fungi which can adapt to various growth conditions and living niches by providing a broad armory of oxidative and antioxidant enzymes. We release the 34.7 Mbp draft genome of Chaetomium cochliodes CCM F-232 consisting of 6036 contigs with an average size of 5756 bp and reconstructed its phylogeny. We show that this filamentous fungus is closely related but not identical ...
Tópico(s): Microbial Metabolism and Applications
2016 - BioMed Central | BMC Genomics
Barbara Blasi, Caroline Poyntner, Tamara Rudavsky, Francesc X. Prenafeta‐Boldú, Sybren de Hoog, Hakim Tafer, Katja Sterflinger,
A collection of 163 strains of black yeast-like fungi from the CBS Fungal Biodiversity Center (Utrecht, The Netherlands), has been screened for the ability to grow on hexadecane, toluene and polychlorinated biphenyl 126 (PCB126) as the sole carbon and energy source. These compounds were chosen as representatives of relevant environmental pollutants. A microtiter plate-based culture assay was set up in order to screen the fungal strains for growth on the selected xenobiotics versus glucose, as a positive ...
Tópico(s): Lichen and fungal ecology
2016 - Taylor & Francis | Geomicrobiology Journal
Caroline Poyntner, Barbara Blasi, Elsa Arcalís, Ursula Mirastschijski, Katja Sterflinger, Hakim Tafer,
The black yeast Exophiala dermatitidis is a widespread polyextremophile and human pathogen, that is found in extreme natural habitats and man-made environments such as dishwashers. It can cause various diseases ranging from phaeohyphomycosis and systemic infections, with fatality rates reaching 40%. While the number of cases in immunocompromised patients are increasing, knowledge of the infections, virulence factors and host response is still scarce. In this study, for the first time, an artificial ...
Tópico(s): Antifungal resistance and susceptibility
2016 - Frontiers Media | Frontiers in Cellular and Infection Microbiology
Zakaria Jalal, Xavier Iriart, Marie–Lou Dinet, Jean‐Bernard Selly, Nadir Tafer, Pauline Renou, Igor Sibon, Jean‐Benoît Thambo,
Percutaneous left atrial appendage (LAA) closure has emerged as an alternative therapeutic option for the prevention of embolic stroke in high-risk patients with non-valvular atrial fibrillation. The presence of thrombus in the LAA is currently a contraindication to the procedure. To describe a modified LAA closure technique that allows a safe procedure in patients with LAA thrombus. Between May 2013 and October 2014, LAA closure was performed in three patients with LAA thrombus (mean age 73.6 ± 14 ...
Tópico(s): Cardiac tumors and thrombi
2016 - Elsevier BV | Archives of cardiovascular diseases
Radia Tafer, Mohamad Sleiman, Abdelaziz Boulkamh, Claire Richard,
The photolysis of aqueous 5-chlorosalicylic acid (ClSA) and dihydroxybenzoic acid (DHBA), its main photoproduct, was studied to determine the extent of degradation caused by simulated solar light. Photoproducts identification was achieved using high resolution LC-MS and GC-MS. About 40 photoproducts from C19 to C1 were characterized, including a dihydroxycyclopentadienic acid, a ring contraction photoproduct, and numerous carbonyls and carboxylic acid derivatives that were detected thanks to derivatization. ...
Tópico(s): Marine and coastal ecosystems
2016 - Elsevier BV | Water Research
Pierre‐Emmanuel Séguéla, François Roubertie, Bernard Kreitmann, Philippe Mauriat, Nadir Tafer, Zakaria Jalal, Jean‐Benoît Thambo,
As preoperative morbi-mortality remains significant, care of newborns with transposition of the great arteries is still challenging. In this review of the literature, we discuss the different treatments that could improve the patient's condition into the preoperative period. Instead of a standardized management, we advocate personalized care of these neonates. Considering the deleterious effects of hypoxia, special attention is given to the use of non-invasive technologies to assess oxygenation ...
Tópico(s): Renal and Vascular Pathologies
2016 - Elsevier BV | Archives of cardiovascular diseases
Guadalupe Piñar, Hakim Tafer, Katja Sterflinger, Flavia Pinzari,
Summary L eonardo da V inci's self‐portrait is affected by foxing spots. The portrait has no fungal or bacterial infections in place, but is contaminated with airborne spores and fungal material that could play a role in its disfigurement. The knowledge of the nature of the stains is of great concern because future conservation treatments should be derived from scientific investigations. The lack of reliable scientific data, due to the non‐culturability of the microorganisms inhabiting the portrait, ...
Tópico(s): Conservation Techniques and Studies
2015 - Wiley | Environmental Microbiology Reports
Barbara Blasi, Hakim Tafer, Donatella Tesei, Katja Sterflinger,
Exophiala dermatitidis (Wangiella dermatitidis) belongs to the group of the so-called black yeasts. Thanks in part to its thick and strongly melanized cell walls, E. dermatitidis is extremely tolerant to various kinds of stress, including extreme pH, temperature and desiccation. E. dermatitidis is also the agent responsible for various severe illnesses in humans, such as pneumonia and keratitis, and might lead to fatal brain infections. Due to its association with the human environment, its poly- ...
Tópico(s): Fungal Infections and Studies
2015 - Public Library of Science | PLoS ONE
Pierre‐Emmanuel Séguéla, Philippe Mauriat, Jean-Baptiste Mouton, Nadir Tafer, Jana Assy, Géraldine Poncelet, Karine Nubret, Xavier Iriart, Jean‐Benoît Thambo,
Children with dilated cardiomyopathy in advanced heart failure may spend a long time awaiting heart transplantation. Consequently, mechanical circulatory support is sometimes required as a bridge to transplantation. Levosimendan, a positive inotropic agent, has been reported to be safe and efficient for the treatment of paediatric heart failure.To report our experience with levosimendan in children with decompensated dilated cardiomyopathy.Paediatric patients with dilated cardiomyopathy on the transplant ...
Tópico(s): Cardiac pacing and defibrillation studies
2015 - Elsevier BV | Archives of cardiovascular diseases
Donatella Tesei, Gorji Marzban, Martina Marchetti‐Deschmann, Hakim Tafer, Elsa Arcalís, Katja Sterflinger,
The black yeast Exophiala dermatitidis is a worldwide distributed agent of primary and secondary diseases in both immunocompromised and healthy humans, with a high prevalence in human-made environments. Since thermo-tolerance has a crucial role in the fungus persistence in man-dominated habitat and in its pathogenicity, three incubation temperatures (37, 45, 1 °C) and two time spans (1 h, 1 week) were selected to simulate different environmental conditions and to investigate the effect of temperature ...
Tópico(s): Antifungal resistance and susceptibility
2015 - Elsevier BV | Journal of Proteomics
Jörg Ettenauer, Guadalupe Piñar, Hakim Tafer, Katja Sterflinger,
The traditional methodology used for the identification of microbes colonizing our cultural heritage was the application of cultivation methods and/or microscopy. This approach has many advantages, as living microorganisms may be obtained for physiological investigations. In addition, these techniques allow the quantitative and qualitative assessment of the investigated environment. Quantitative analyses are done by plate count and the determination of abundance by the colony forming unit (CFU). ...
Tópico(s): Indoor Air Quality and Microbial Exposure
2014 - Frontiers Media | Frontiers in Microbiology
Christian Anthon, Hakim Tafer, Jakob H. Havgaard, Bo Thomsen, Jakob Hedegaard, Stefan E. Seemann, Sachin Pundhir, Stephanie Kehr, Sebastian Bartschat, Mathilde Nielsen, Rasmus Oestergaard Nielsen, Merete Fredholm, Peter F. Stadler, Jan Gorodkin,
Abstract Background Annotating mammalian genomes for noncoding RNAs (ncRNAs) is nontrivial since far from all ncRNAs are known and the computational models are resource demanding. Currently, the human genome holds the best mammalian ncRNA annotation, a result of numerous efforts by several groups. However, a more direct strategy is desired for the increasing number of sequenced mammalian genomes of which some, such as the pig, are relevant as disease models and production animals. Results We present ...
Tópico(s): RNA and protein synthesis mechanisms
2014 - BioMed Central | BMC Genomics