Melissa G. Mitchum, Shinjiro Yamaguchi, Atsushi Hanada, Ayuko Kuwahara, Yasushi Yoshioka, Tomohiko Kato, Satoshi Tabata, Yuji Kamiya, Tai‐ping Sun,
Summary Gibberellin (GA) 3‐oxidase, a class of 2‐oxoglutarate‐dependent dioxygenases, catalyzes the conversion of precursor GAs to their bioactive forms, thereby playing a direct role in determining the levels of bioactive GAs in plants. Gibberellin 3‐oxidase in Arabidopsis is encoded by a multigene family consisting of at least four members, designated AtGA3ox1 to AtGA3ox4 . It has yet to be investigated how each AtGA3ox gene contributes to optimizing bioactive GA levels during growth and development. ...
Tópico(s): Plant Stress Responses and Tolerance
2006 - Wiley | The Plant Journal
Christine M. Fleet, Shinjiro Yamaguchi, Atsushi Hanada, Hiroshi Kawaide, Charles J. David, Yuji Kamiya, Tai‐ping Sun,
Abstract The plant growth hormone gibberellin (GA) is important for many aspects of plant growth and development. Although most genes encoding enzymes at each step of the GA biosynthetic pathway have been cloned, their regulation is less well understood. To assess how up-regulation of early steps affects the biosynthetic pathway overall, we have examined transgenic Arabidopsis plants that overexpress either AtCPS or AtKS or both. These genes encode the enzymes ent-copalyl diphosphate synthase (CPS) ...
Tópico(s): Plant Reproductive Biology
2003 - Oxford University Press | PLANT PHYSIOLOGY
Jianhong Hu, Melissa G. Mitchum, Neel G. Barnaby, Belay T. Ayele, Mikihiro Ogawa, Edward A. Nam, Wei-Chu Victoria Lai, Atsushi Hanada, José M. Alonso, Joseph R. Ecker, Stephen M. Swain, Shinjiro Yamaguchi, Yuji Kamiya, Tai‐ping Sun,
Abstract Gibberellin 3-oxidase (GA3ox) catalyzes the final step in the synthesis of bioactive gibberellins (GAs). We examined the expression patterns of all four GA3ox genes in Arabidopsis thaliana by promoter–β-glucuronidase gene fusions and by quantitative RT-PCR and defined their physiological roles by characterizing single, double, and triple mutants. In developing flowers, GA3ox genes are only expressed in stamen filaments, anthers, and flower receptacles. Mutant plants that lack both GA3ox1 ...
Tópico(s): Plant Physiology and Cultivation Studies
2008 - Oxford University Press | The Plant Cell
Rodolfo Zentella, Zhonglin Zhang, Me‐Hea Park, Stephen G. Thomas, Akira Endo, Kohji Murase, Christine M. Fleet, Yusuke Jikumaru, Eiji Nambara, Yuji Kamiya, Tai‐ping Sun,
Bioactive gibberellins (GAs) are phytohormones that regulate growth and development throughout the life cycle of plants. DELLA proteins are conserved growth repressors that modulate all aspects of GA responses. These GA-signaling repressors are nuclear localized and likely function as transcriptional regulators. Recent studies demonstrated that GA, upon binding to its receptor, derepresses its signaling pathway by binding directly to DELLA proteins and targeting them for rapid degradation via the ...
Tópico(s): Plant Stress Responses and Tolerance
2007 - Oxford University Press | The Plant Cell
Aron L. Silverstone, Hou-Sung Jung, Alyssa Dill, Hiroshi Kawaide, Yuji Kamiya, Tai‐Ping Sun,
Tópico(s): Polysaccharides and Plant Cell Walls
2001 - Oxford University Press | The Plant Cell
Aron L. Silverstone, Hou-Sung Jung, Alyssa Dill, Hiroshi Kawaide, Yuji Kamiya, Tai‐ping Sun,
RGA (for repressor of ga1-3) and SPINDLY (SPY) are likely repressors of gibberellin (GA) signaling in Arabidopsis because the recessive rga and spy mutations partially suppressed the phenotype of the GA-deficient mutant ga1-3. We found that neither rga nor spy altered the GA levels in the wild-type or the ga1-3 background. However, expression of the GA biosynthetic gene GA4 was reduced 26% by the rga mutation, suggesting that partial derepression of the GA response pathway by rga resulted in the feedback ...
Tópico(s): Polysaccharides and Plant Cell Walls
2001 - Oxford University Press | The Plant Cell
Shinjiro Yamaguchi, Yuji Kamiya, Tai‐ping Sun,
Summary Gibberellins (GAs) are biosynthesized through a complex pathway that involves several classes of enzymes. To predict sites of individual GA biosynthetic steps, we studied cell type‐specific expression of genes encoding early and late GA biosynthetic enzymes in germinating Arabidopsis seeds. We showed that expression of two genes, AtGA3ox1 and AtGA3ox2 , encoding GA 3‐oxidase, which catalyzes the terminal biosynthetic step, was mainly localized in the cortex and endodermis of embryo axes in ...
Tópico(s): Plant Stress Responses and Tolerance
2001 - Wiley | The Plant Journal
Xin Zhou, Zhonglin Zhang, Jeongmoo Park, Ludmila Tyler, Yusuke Jikumaru, Kai Qiu, Edward A. Nam, Shelley Lumba, Darrell Desveaux, Peter McCourt, Yuji Kamiya, Tai‐ping Sun,
The phytohormone gibberellin (GA) plays a key role in promoting stem elongation in plants. Previous studies show that GA activates its signaling pathway by inducing rapid degradation of DELLA proteins, GA signaling repressors. Using an activation-tagging screen in a reduced-GA mutant ga1-6 background, we identified AtERF11 to be a novel positive regulator of both GA biosynthesis and GA signaling for internode elongation. Overexpression of AtERF11 partially rescued the dwarf phenotype of ga1-6. AtERF11 ...
Tópico(s): Plant Stress Responses and Tolerance
2016 - Oxford University Press | PLANT PHYSIOLOGY
Shinjiro Yamaguchi, M. W. Smith, Robert G. Brown, Yuji Kamiya, Tai‐Ping Sun,
Tópico(s): Leaf Properties and Growth Measurement
1998 - Oxford University Press | The Plant Cell
Shinjiro Yamaguchi, M. W. Smith, Robert G. Brown, Yuji Kamiya, Tai‐ping Sun,
Despite extensive studies on the roles of phytochrome in photostimulated seed germination, the mechanisms downstream of the photoreceptor that promote germination are largely unknown. Previous studies have indicated that light-induced germination of Arabidopsis seeds is mediated by the hormone gibberellin (GA). Using RNA gel blot analyses, we studied the regulation of two Arabidopsis genes, GA4 and GA4H (for GA4 homolog), both of which encode GA 3β-hydroxylases that catalyze the final biosynthetic ...
Tópico(s): Plant Molecular Biology Research
1998 - Oxford University Press | The Plant Cell
Zhong-Lin Zhang, Mikihiro Ogawa, Christine M. Fleet, Rodolfo Zentella, Jianhong Hu, Jung-Ok Heo, Jun Lim, Yuji Kamiya, Shinjiro Yamaguchi, Tai‐ping Sun,
The diterpenoid phytohormone gibberellin (GA) controls diverse developmental processes throughout the plant life cycle. DELLA proteins are master growth repressors that function immediately downstream of the GA receptor to inhibit GA signaling. By doing so, DELLAs also play pivotal roles as integrators of internal developmental signals from multiple hormone pathways and external cues. DELLAs are likely nuclear transcriptional regulators, which interact with other transcription factors to modulate ...
Tópico(s): Photosynthetic Processes and Mechanisms
2011 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Atiako Kwame Acheampong, Jianhong Hu, Ariel Rotman, Chuanlin Zheng, Tamar Halaly, Yumiko Takebayashi, Yusuke Jikumaru, Yuji Kamiya, Amnon Lichter, Tai‐ping Sun, Etti Or,
Gibberellins (GAs) regulate numerous developmental processes in grapevine (Vitis vinifera) such as rachis elongation, fruit set, and fruitlet abscission. The ability of GA to promote berry enlargement has led to its indispensable use in the sternospermocarpic ('seedless') table grape industry worldwide. However, apart from VvGAI1 (VvDELLA1), which regulates internode elongation and fruitfulness, but not berry size of seeded cultivars, little was known about GA signalling in grapevine. We have identified ...
Tópico(s): Plant Reproductive Biology
2015 - Oxford University Press | Journal of Experimental Botany
Tópico(s): Plant Diversity and Evolution
1994 - Oxford University Press | The Plant Cell
The first committed step in the gibberellin (GA) biosynthetic pathway is the conversion of geranylgeranyl pyrophosphate (GGPP) through copalyl pyrophosphate (CPP) to ent-kaurene catalyzed by ent-kaurene synthetases A and B. The ga1 mutants of Arabidopsis are gibberellin-responsive male-sterile dwarfs. Biochemical studies indicate that biosynthesis of GAs in the ga1 mutants is blocked prior to the synthesis of ent-kaurene. The GA1 locus was cloned previously using the technique of genomic subtraction. ...
Tópico(s): Photosynthetic Processes and Mechanisms
1994 - Oxford University Press | The Plant Cell
Eunkyoo Oh, Shinjiro Yamaguchi, Jianhong Hu, Yusuke Jikumaru, Byunghyuck Jung, Inyup Paik, Hee‐Seung Lee, Tai‐ping Sun, Yuji Kamiya, Giltsu Choi,
Abstract Previous work showed that PHYTOCHROME-INTERACTING FACTOR3-LIKE5 (PIL5), a light-labile basic helix-loop-helix protein, inhibits seed germination by repressing GIBBERELLIN 3β-HYDROXYLASE1 (GA3ox1) and GA3ox2 and activating a gibberellic acid (GA) catabolic gene (GA2ox2). However, we show persistent light-dependent and PIL5-inhibited germination behavior in the absence of both de novo GA biosynthesis and deactivation by GA2ox2, suggesting that PIL5 regulates not only GA metabolism but also GA ...
Tópico(s): Seed Germination and Physiology
2007 - Oxford University Press | The Plant Cell
Akihito Chugun, Kyosuke Temma, Toshifumi OYAMADA, Natsuyo Suzuki, Yoshinori Kamiya, Yukio Hara, Takushi Sasaki, Hiroshi Kondo, Tai Akera,
Doxorubicin treatment causes delayed development of cardiotoxicity. Whether the doxorubicin-induced impairment of cardiac functions reverses or progresses with time after the cessation of the treatment was examined. The rats were injected with doxorubicin (2.5 mg/kg, i.v., once a week for 3 weeks) and sacrificed at 1 (1W), 13 (13W), or 18 (18W) weeks after the final doxorubicin administration. The time to peak of twitch contraction observed at 2-Hz stimulation was not altered in left atrial or ventricular ...
Tópico(s): Carbon Nanotubes in Composites
2000 - NRC Research Press | Canadian Journal of Physiology and Pharmacology
The two‐step cyclization reaction of ent ‐kaurene synthesis from geranylgeranyl diphosphate is the first committed step in the biosynthetic pathway of the plant hormone gibberellin. Recent molecular cloning and characterization of the genes encoding the two corresponding enzymes, copalyl diphosphate synthase (CPS) and ent ‐kau‐rene synthase (KS), have demonstrated that ent ‐kaurene synthesis is localized in the plastids and is highly regulated in specific tissues and cell types during plant development. ...
Tópico(s): Plant Gene Expression Analysis
1997 - Wiley | Physiologia Plantarum
Tahar Aït-Ali, Stephen M. Swain, James B. Reid, Tai‐ping Sun, Yuji Kamiya,
Summary Gibberellins (GAs) are hormones required for several aspects of plant development, including internode elongation and seed development in pea ( Pisum sativum L.). The first committed step in the GA biosynthesis pathway is the conversion of geranylgeranyl diphosphate (GGDP) to ent ‐kaurene via copalyl diphosphate (CDP). These two reactions are catalyzed by the cyclases ent ‐kaurene synthase A (KSA) and ent ‐kaurene synthase B (KSB), respectively. Previous genetic and biochemical analysis of the GA‐ ...
Tópico(s): Plant Stress Responses and Tolerance
1997 - Wiley | The Plant Journal
Akihito Chugun, Kyosuke Temma, Toshifumi OYAMADA, Natsuyo Suzuki, Yoshinori Kamiya, Yukio Hara, Takushi Sasaki, Hiroshi Kondo, Tai Akera,
Doxorubicin treatment causes delayed development of cardiotoxicity. Whether the doxorubicin-induced impairment of cardiac functions reverses or progresses with time after the cessation of the treatment was examined. The rats were injected with doxorubicin (2.5 mg/kg, i.v., once a week for 3 weeks) and sacrificed at 1 (1W), 13 (13W), or 18 (18W) weeks after the final doxorubicin administration. The time to peak of twitch contraction observed at 2-Hz stimulation was not altered in left atrial or ventricular ...
Tópico(s): Carbon Nanotubes in Composites
2000 - NRC Research Press | Canadian Journal of Physiology and Pharmacology
Mitsunori Seo, Atsushi Hanada, Ayuko Kuwahara, Akira Endo, Masanori Okamoto, Yukika Yamauchi, Helen North, Annie Marion‐Poll, Tai‐ping Sun, Tomokazu Koshiba, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara,
In a wide range of plant species, seed germination is regulated antagonistically by two plant hormones, abscisic acid (ABA) and gibberellin (GA). In the present study, we have revealed that ABA metabolism (both biosynthesis and inactivation) was phytochrome-regulated in an opposite fashion to GA metabolism during photoreversible seed germination in Arabidopsis. Endogenous ABA levels were decreased by irradiation with a red (R) light pulse in dark-imbibed seeds pre-treated with a far-red (FR) light pulse, ...
Tópico(s): Seed Germination and Physiology
2006 - Wiley | The Plant Journal
Shinjiro Yamaguchi, Tai‐ping Sun, Hiroshi Kawaide, Yuji Kamiya,
The ga2 mutant of Arabidopsis thaliana is a gibberellin-deficient dwarf. Previous biochemical studies have suggested that the ga2 mutant is impaired in the conversion of copalyl diphosphate to ent-kaurene, which is catalyzed by ent-kaurene synthase (KS). Overexpression of the previously isolated KS cDNA from pumpkin (Cucurbita maxima) (CmKS) in the ga2 mutant was able to complement the mutant phenotype. A genomic clone coding for KS, AtKS, was isolated from A. thaliana using CmKS cDNA as a heterologous ...
Tópico(s): Plant Gene Expression Analysis
1998 - Oxford University Press | PLANT PHYSIOLOGY
Shuhei Mayanagi, Hiroya Takeuchi, Satoshi Kamiya, Masahiro Niihara, Rieko Nakamura, Tsunehiro Takahashi, Norihito Wada, Hirofumi Kawakubo, Yoshiro Saikawa, Tai Omori, Tadaki Nakahara, Makio Mukai, Yuko Kitagawa,
Tópico(s): Gastrointestinal Tumor Research and Treatment
2014 - Springer Science+Business Media | Annals of Surgical Oncology
Ayako Shimada, Hiroya Takeuchi, Taiki Ono, Satoshi Kamiya, Kazumasa Fukuda, Rieko Nakamura, Tsunehiro Takahashi, Norihito Wada, Hirofumi Kawakubo, Yoshiro Saikawa, Tai Omori, Tadaki Nakahara, Masahiro Jinzaki, Koji Murakami, Yuko Kitagawa,
Tópico(s): Metastasis and carcinoma case studies
2016 - Springer Science+Business Media | Annals of Surgical Oncology
Shinjiro Yamaguchi, M. W. Smith, Robert Angus Brown, Yuji Kamiya, Sun Tai-ping,
Tópico(s): Phytase and its Applications
1999 - Oxford University Press | Plant and Cell Physiology
Michiyo Kato, Tai Kurosawa, Tetsuji Kamiya,
We developed the Coparental Regulation Inventory to assess the regulatory behavior of the mothers in involving fathers with child rearing. We translated and modified the short form of the Parental Regulation Inventory (PRI) for Japanese couples in different stages of child rearing. An online questionnaire was conducted with mothers (n = 500) and fathers (n = 500) whose youngest child was less than 21-years-old. Exploratory factor analysis identified two factors, which were labeled “encouragement” and “criticism”. ...
Tópico(s): Attachment and Relationship Dynamics
2014 - The Japanese Psychological Association | The Japanese journal of psychology
Satoshi Kamiya, Hiroya Takeuchi, Tadaki Nakahara, Masahiro Niihara, Rieko Nakamura, Tsunehiro Takahashi, Norihito Wada, Hirofumi Kawakubo, Yoshiro Saikawa, Tai Omori, Koji Murakami, Yuko Kitagawa,
Sentinel node (SN) mapping using dye and radioisotope (RI) tracer has been reported to be feasible in cases of early gastric cancer. Because accurate diagnosis of micrometastasis is sometimes difficult in the limited time available during surgery, a faster and simpler method of improving the intraoperative diagnostic precision of lymph node metastasis is needed. The amount of tracer deposited in an SN can be determined from its radioactivity; however, the significance of the RI count has not been ...
Tópico(s): Metastasis and carcinoma case studies
2015 - Springer Science+Business Media | Gastric Cancer
Kyosuke Temma, Akihito Chugun, Natsuyo Suzuki, Kazunori Kamiya, Yukio Hara, Takushi Sasaki, Hiroshi Kondo, Tai Akera,
Tópico(s): Ion channel regulation and function
2000 - Japanese Pharmacological Society | The Japanese Journal of Pharmacology
Tomomichi Suzuki, Kenji Okumura, Takahito Sone, Tai Kosokabe, Hideyuki Tsuboi, Junichiro Kondo, Hiroaki Mukawa, Hiroki Kamiya, Takahito Tomida, Hajime Imai, Hideo Matsui, Tetsuo Hayakawa,
Reduced or impaired synthesis of nitric oxide promotes the proliferation of vascular smooth muscle cells, and thus may induce the neointimal formation leading to coronary in-stent restenosis. Recent reports have suggested that the Glu298Asp polymorphism in exon 7 of the endothelial nitric oxide synthase gene is associated with coronary spasm and acute myocardial infarction. In this study, we have examined the implication of this polymorphism with regard to coronary restenosis after Palmaz-Schatz ...
Tópico(s): Cardiovascular Function and Risk Factors
2002 - Elsevier BV | International Journal of Cardiology
Junichiro Kondo, T. Sone, Hideyuki Tsuboi, Hiroaki Mukawa, Tai Kosokabe, Michitaka Tsuzuki, Takahito Tomida, Tomomichi Suzuki, Hiroki Kamiya, Kazunori Hayashi, Hideo Matsui, Kenji Okumura,
We studied whether angiotensin-converting enzyme inhibition with quinapril treatment can prevent in-stent restenosis after successful implantation of Palmaz-Schatz stents. Intravascular ultrasound study, but not quantitative coronary angiography analysis, revealed that quinapril treatment significantly prevented the loss of both minimal lumen cross-sectional area and lumen volume in stents, in addition to reducing the increase in intimal hyperplasia volume.
Tópico(s): Acute Myocardial Infarction Research
2001 - Elsevier BV | The American Journal of Cardiology
Nobuhiko Chikamoto, Tai-ichiro Chikama, Naoyuki Yamada, Teruo Nishida, Tadashi Ishimitsu, Akira Kamiya,
The efficacy of eyedrops containing peptides based on substance P (FGLM-amide) and insulinlike growth factor-1 (SSSR) for prevention of superficial punctate keratopathy (SPK) after cataract surgery in individuals with type 2 diabetes was examined. Twenty-nine patients (29 eyes) with diabetes were enrolled in a double-masked, prospective, randomized, placebo-controlled clinical study. They were randomly assigned to one of two groups that received eyedrops containing either FGLM-amide and SSSR (n = 14) or ...
Tópico(s): Ocular Surface and Contact Lens
2009 - Springer Science+Business Media | Japanese Journal of Ophthalmology