Tomohisa Mori, Teruo Hayashi, Eri Hayashi, Tsung‐Ping Su,
The membrane of the endoplasmic reticulum (ER) of a cell forms contacts directly with mitochondria whereby the contact is referred to as the mitochondrion-associated ER membrane or the MAM. Here we found that the MAM regulates cellular survival via an MAM-residing ER chaperone the sigma-1 receptor (Sig-1R) in that the Sig-1R chaperones the ER stress sensor IRE1 to facilitate inter-organelle signaling for survival. IRE1 is found in this study to be enriched at the MAM in CHO cells. We found that IRE1 ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2013 - Public Library of Science | PLoS ONE
Teruo Hayashi, Li‐I Tsao, Tsung‐Ping Su,
Abstract The delta opioid peptide [D‐Ala 2 ,D‐Leu 5 ]enkephalin (DADLE) has been shown to promote organ survival and to protect against methamphetamine‐induced neurodegeneration. However, the cellular mechanisms of these actions of DADLE are not totally clear. We examined the action of DADLE in serum‐deprived pheochromocytoma cells (PC12) and found that DADLE protected against cell death in those cells. However, the dose–response curves of the protective effects of DADLE are U‐shaped as judged by three ...
Tópico(s): Neuroscience and Neuropharmacology Research
2001 - Wiley | Synapse
Yun Wang, Teruo Hayashi, Chen‐Fu Chang, Yung‐Hsiao Chiang, Li-I Tsao, Tsung‐Ping Su, Cesar V. Borlongan, Shinn‐Zong Lin,
Background and Purpose —Previous studies have indicated that both methamphetamine (MA) and ischemia/reperfusion injuries involve reactive oxygen species formation and activation of apoptotic mechanism. That MA could have a synergistic or additive effect with stroke-induced brain damage is possible. The purpose of the present study was to investigate whether administration of MA in vivo would potentiate ischemic brain injury. Methods —Adult CD-1 mice were pretreated with MA or saline. Each animal later ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2001 - Lippincott Williams & Wilkins | Stroke
Cytoskeletal proteins are important in protein trafficking, membrane protein clustering, dendrite growth and the morphological maintenance of neurons. Sigma(1) receptors are unique endoplasmic reticular (ER) proteins that bind (+)benzomorphans, neurosteroids and psychotropic drugs such as cocaine. Cocaine, via sigma(1) receptors, can cause the dissociation of a cytoskeletal adaptor protein ankyrin from inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3)] receptors on the ER as a sigma(1)-receptor-ankyrin complex, ...
Tópico(s): Nicotinic Acetylcholine Receptors Study
2001 - Elsevier BV | Trends in Pharmacological Sciences
Ankyrin is a cytoskeletal adaptor protein that controls important cellular functions, including Ca 2+ efflux at inositol 1,4,5-trisphosphate receptors (IP 3 R) on the endoplasmic reticulum. The present study found that sigma-1 receptors (Sig-1R), unique endoplasmic reticulum proteins that bind certain steroids, neuroleptics, and psychotropic drugs, form a trimeric complex with ankyrin B and IP 3 R type 3 (IP 3 R-3) in NG-108 cells. The trimeric complex could be coimmunoprecipitated by antibodies against ...
Tópico(s): Neuropeptides and Animal Physiology
2001 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Teruo Hayashi, Hiroshi Hirata, Masato Asanuma, Bruce Ladenheim, Li‐I Tsao, Jean Lud Cadet, Tsung‐Ping Su,
The delta opioid peptide [D-Ala2, D-Leu5]enkephalin (DADLE) has been reported to block the neurotoxicity induced by multiple administrations of a moderate dose of methamphetamine (METH). We examined in this study if DADLE might block the neurotoxicity caused by a single high dose of METH in CD-1 mice. The levels of dopamine transporter (DAT), tyrosine hydroxylase (TH), major biogenic amines including DA, 5-hydroxytryptamine (5-HT), and their metabolites were examined. In addition, since the tumor suppressor ...
Tópico(s): Cancer-related Molecular Pathways
2001 - Wiley | Synapse
Takeshi Miyazawa, Shunji Takahashi, Akihiro Kawata, Suresh Panthee, Teruo Hayashi, Takeshi Shimizu, Toshihiko Nogawa, Hiroyuki Osada,
Understanding the biosynthetic mechanism of the atypical polyketide extender unit is important for the development of bioactive natural products. Reveromycin (RM) derivatives produced by Streptomyces sp. SN-593 possess several aliphatic extender units. Here, we studied the molecular basis of 2-alkylmalonyl-CoA formation by analyzing the revR and revS genes, which form a transcriptional unit with the revT gene, a crotonyl-CoA carboxylase/reductase homolog. We mainly focused on the uncharacterized ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2015 - Elsevier BV | Journal of Biological Chemistry
Teruo Hayashi, Hiroshi Kakisawa, Shô Itô, Yuh Pan Chen, Hong-Yen Hsū,
Tópico(s): Turfgrass Adaptation and Management
1972 - Elsevier BV | Tetrahedron Letters
Teruo Hayashi, Teiki Iwaoka, Noriko Takeda, Eiji Ohki,
3-Dithiocarbonates (1d, 1e, 3a) and 3, 4-bis analog (1b) derived from methyl 2, 6-dibenzyloxycarbonylamino-2, 6-dideoxy-α-D-glucopyranoside (1a) were converted into 3-deoxy compounds (1c, 1f, 3b) and 3-eno compound (2) respectively in good yield on treatment with tributylstannane. This reaction process was extended into the field of aminoglycoside antibiotics, giving 5-deoxykanamycin B (8a), 3', 4', 5-trideoxykanamycin B (8c), 3', 4', 5-trideoxy-3'-enokanamycin B (8b), 6-deoxybutirosin A (9a), and 3'-deoxybutirosin ...
Tópico(s): Microbial Natural Products and Biosynthesis
1978 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Teruo Hayashi, Ronald H. Thomson,
The wood of Conocarpus erectus contains conocarpol and 2′-methoxyconocarpol, simple 1,4-diarylbutane-type lignans, and conocarpan, a lignan of the dehydrodi-isoeugenol type.
Tópico(s): Remote Sensing and Land Use
1975 - Elsevier BV | Phytochemistry
Teruo Hayashi, Ronald H. Thomson,
Tópico(s): Traditional and Medicinal Uses of Annonaceae
1974 - Elsevier BV | Phytochemistry
S. OIDA, Akira Yoshida, Teruo Hayashi, Eiji Nakayama, Sadao Sato, Eiji Ohki,
Abstract The structure for the fused β-lactam obtained by treatment of the secolactam 3 with base in the presence of Cu(I)-ion is not the penem 1 (R=CH 3 ), but the isopenem 4 on the basis of X-ray analysis.
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
1980 - Elsevier BV | Tetrahedron Letters
Teruo Hayashi, Akira Yoshida, Noriko Takeda, S. OIDA, SHINICHI SUGAWARA, Eiji Ohki,
The synthesis of optically active "1-thiathienamycin"13a and other (hydroxyethyl)-penemcarboxylic acids from the 8R and 8S azetidinones 3 and 4 via an intramolecular Wittig reaction of the trithiocarbonatephosphoranes 7 is described. Trans and cis penem esters 8 and 9 were found to form an equilibrium mixture on heating. The antibacterial activities of these penemcarboxylic acids are discussed.
Tópico(s): Synthesis and Reactivity of Sulfur-Containing Compounds
1981 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Akira Yoshida, Teruo Hayashi, Noriko Takeda, S. OIDA, Eiji Ohki,
The synthesis of hydroxyethylazetidinone precursors to 1-thia analogs of thienamycin is described. An N-protected azetidinone 4 was hydroxyethylated via an aldol reaction to give four diastereomers 5-8, which were converted to the diastereomeric pair of racemic 8R and 8S trans azetidinones 9b and 10b. Stereochemical assignment was achieved by correlation of 9b with the optically active azetidinone 16 which was obtained from the hydroxyethylpenicillanate 14a with known stereochemistry. The optically ...
Tópico(s): Carbohydrate Chemistry and Synthesis
1981 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Akira Yoshida, Teruo Hayashi, Noriko Takeda, S. OIDA, Eiji Ohki,
Treatment of the oxalimide 5 with triethyl phosphite formed the penem 3 and the triethoxyphosphonium ylide 7, presumably via the common carbene intermediate 10. Prolonged heating of 7 afforded 3 and its C-5 epimer. Similar reaction of the oxalimide 13 with trimethyl phosphite gave only the ylide 14, which was further heated to cyclize to the penem 15.
Tópico(s): Synthetic Organic Chemistry Methods
1983 - Pharmaceutical Society of Japan | Chemical and Pharmaceutical Bulletin
Takenori Kusumi, Takashi Ooi, Teruo Hayashi, Hiroshi Kakisawa,
Tópico(s): Phytochemistry and Biological Activities
1985 - Elsevier BV | Phytochemistry
Tsung‐Ping Su, Teruo Hayashi, D. Bruce Vaupel,
An endogenous hallucinogenic compound binds to a ligand-gated chaperone.
Tópico(s): Tryptophan and brain disorders
2009 - American Association for the Advancement of Science | Science Signaling
Shang‐Yi Tsai, Teruo Hayashi, Brandon K. Harvey, Yun Wang, Wells W. Wu, Rong‐Fong Shen, Yongqing Zhang, Kevin G. Becker, Barry J. Hoffer, Tsung‐Ping Su,
Sigma-1 receptors (Sig-1Rs) are endoplasmic reticulum (ER)-resident proteins known to be involved in learning and memory. Dendritic spines in hippocampal neurons play important roles in neuroplasticity and learning and memory. This study tested the hypothesis that Sig-1Rs might regulate denritic spine formation in hippocampal neurons and examined potential mechanisms therein. In rat hippocampal primary neurons, the knockdown of Sig-1Rs by siRNAs causes a deficit in the formation of dendritic spines ...
Tópico(s): Receptor Mechanisms and Signaling
2009 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Cesar V. Borlongan, Teruo Hayashi, Peter R. Oeltgen, Tsung‐Ping Su, Yun Wang,
Delta opioid peptide [D-ala2,D-leU5]enkephalin (DADLE) induces hibernation in summer ground squirrels, and enhances preservation and survival of isolated or transplanted lungs and hearts. In the present study, we investigated the protective effect of DADLE in the central nervous system. Adult Sprague-Dawley rats were pretreated with DADLE (4 mg/kg every 2 h × 4 injections, i.p.) or saline prior to unilateral occlusion of the middle cerebral artery (MCA). Daily behavioral tests revealed that ischemic animals ...
Tópico(s): Biochemical effects in animals
2009 - BioMed Central | BMC Biology
Johann Meunier, Teruo Hayashi,
The expression of Bcl-2, the major antiapoptotic member of the Bcl-2 family, is under complex controls of several factors, including reactive oxygen species (ROS). The σ-1 receptor (Sig-1R), which was recently identified as a novel molecular chaperone at the mitochondria-associated endoplasmic reticulum membrane (MAM), has been shown to exert robust cellular protective actions. However, mechanisms underlying the antiapoptotic action of the Sig-1R remain to be clarified. Here, we found that the Sig- ...
Tópico(s): Sphingolipid Metabolism and Signaling
2009 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Chun‐Ting Lee, Elin Lehrmann, Teruo Hayashi, Rose Amable, Shang‐Yi Tsai, Jia Chen, Joseph F. Sanchez, James Shen, Kevin G. Becker, William J. Freed,
Objectives: Prenatal exposure to cocaine causes cytoarchitectural alterations in the developing neocortex. Previously, we reported that cocaine inhibits neural progenitor cell proliferation through oxidative endoplasmic reticulum stress and consequent down-regulation of cyclin A, whereas cyclin A expression was increased in astrocytes. In the present study, cell type-specific responses to cocaine were further explored. Methods: Gene expression profiles were examined in 5 types of cells obtained ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2009 - Lippincott Williams & Wilkins | Journal of Addiction Medicine
Teruo Hayashi, Rosario Rizzuto, György Hajnóczky, Tsung‐Ping Su,
The physical association between the endoplasmic reticulum (ER) and mitochondria, which is known as the mitochondria-associated ER membrane (MAM), has important roles in various cellular ‘housekeeping' functions including the non-vesicular transports of phospholipids. It has recently become clear that the MAM also enables highly efficient transmission of Ca 2+ from the ER to mitochondria to stimulate oxidative metabolism and, conversely, might enable the metabolically energized mitochondria to regulate ...
Tópico(s): Mitochondrial Function and Pathology
2009 - Elsevier BV | Trends in Cell Biology
Shang‐Yi Tsai, Teruo Hayashi, Tomohisa Mori, Tsung‐Ping Su,
Chaperones are proteins that assist the correct folding of other protein clients either when the clients are being synthesized or at their functional localities. Chaperones are responsible for certain diseases. The sigma-1 receptor is recently identified as a receptor chaperone whose activity can be activated/deactivated by specific ligands. Under physiological conditions, the sigma-1 receptor chaperones the functional IP3 receptor at the endoplasmic reticulum and mitochondrion interface to ensure ...
Tópico(s): Computational Drug Discovery Methods
2009 - Bentham Science Publishers | Central Nervous System Agents in Medicinal Chemistry
Teruo Hayashi, Zuzana Justinová, Eri Hayashi, Gianfrancesco Cormaci, Tomohisa Mori, Shang‐Yi Tsai, Chanel Barnes, Steven R. Goldberg, Tsung‐Ping Su,
σ-1 Receptors are endoplasmic reticulum (ER) chaperones that are implicated in the neuroplasticity associated with psychostimulant abuse. We immunocytochemically examined the distribution of σ-1 receptors in the brain of drug-naive rats and then examined the dynamics of σ-1 receptors and other ER chaperones in specific brain subregions of rats that self-administered methamphetamine, received methamphetamine passively, or received only saline injections. σ-1 Receptors were found to be expressed in ...
Tópico(s): Tryptophan and brain disorders
2009 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Minoru Takebayashi, Teruo Hayashi, Tsung‐Ping Su,
One theory concerning the action of antidepressants relates to the drugs9 ability to induce an adaptive plasticity in neurons such as neurite sprouting. Certain antidepressants are known to bind to ς-1 receptors (Sig-1R) with high affinity. Sig-1R are dynamic endoplasmic reticulum proteins that are highly concentrated at the tip of growth cones in cultured cells. We therefore tested the hypotheses that Sig-1R might participate in the neurite sprouting and that antidepressants with Sig-1R affinity ...
Tópico(s): Receptor Mechanisms and Signaling
2002 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Although sigma receptors were discovered in 1982, the biochemical and physiological roles of sigma receptors have just begun to unveil.Sigma receptors are non-opioid, non-phencyclidine receptors that contain two subtypes: sigma-1 and sigma-2 receptors.The sigma-1 receptor has been cloned and its sequence does not resemble that of any mammalian protein.Sigma-2 receptors have not been cloned.The focus of this review will be on sigma-1 receptors.Sigma-1 receptors contain 223 amino acids and reside ...
Tópico(s): Neuropeptides and Animal Physiology
2003 - Bentham Science Publishers | Current Medicinal Chemistry
The brain σ-1 receptors can bind neurosteroids and psychotropic drugs, including neuroleptics and cocaine and are implicated in schizophrenia, depression, and drug dependence. In this study, we found that σ-1 receptors specifically target lipid storage sites (lipid droplets) on the endoplasmic reticulum by forming a distinct class of lipid microdomains. Both endogenously expressing σ-1 receptors and transfected C-terminally enhanced yellow fluorescent protein (EYFP)-tagged σ-1 receptors (Sig-1R-EYFP) ...
Tópico(s): Neuropeptides and Animal Physiology
2003 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Jeffrey W. Grimm, Lin Lü, Teruo Hayashi, Bruce T. Hope, Tsung‐Ping Su, Yavin Shaham,
Using a rat model of drug craving, we found that the responsiveness to cocaine cues progressively increases or incubates over the first 60 d of cocaine withdrawal. Here we studied whether alterations in brain-derived neurotrophic factor (BDNF) protein levels within the mesolimbic dopamine system are associated with this incubation phenomenon. BDNF is involved in synaptic plasticity and was found to enhance responding for cues associated with natural rewards. Rats were trained to press a lever to ...
Tópico(s): Receptor Mechanisms and Signaling
2003 - Society for Neuroscience | Journal of Neuroscience
The sigma-1 receptors bind diverse kinds of psychoactive compounds, including cocaine, and translocate upon stimulation by these compounds. However, the exact intracellular localization and dynamics of sigma-1 receptors have been unclear. We recently found that sigma-1 receptors specifically localize on cholesterol-enriched loci on the endoplasmic reticulum (ER) membrane that function as neutral lipid storage sites (i.e., ER lipid droplets or ER-LDs) from which neutral lipids bud out to form cytosolic ...
Tópico(s): Neuroscience and Neuropharmacology Research
2003 - American Society for Pharmacology and Experimental Therapeutics | Journal of Pharmacology and Experimental Therapeutics
Chun‐Ting Lee, Jia Chen, Teruo Hayashi, Shang‐Yi Tsai, Joseph F. Sanchez, Stacie L. Errico, Rose Amable, Tsung‐Ping Su, Ross H. Lowe, Marilyn A. Huestis, James Shen, Kevin G. Becker, Herbert M. Geller, William J. Freed,
Prenatal exposure of the developing brain to cocaine causes morphological and behavioral abnormalities. Recent studies indicate that cocaine-induced proliferation inhibition and/or apoptosis in neural progenitor cells may play a pivotal role in causing these abnormalities. To understand the molecular mechanism through which cocaine inhibits cell proliferation in neural progenitors, we sought to identify the molecules that are responsible for mediating the effect of cocaine on cell cycle regulation. ...
Tópico(s): Prenatal Substance Exposure Effects
2008 - Public Library of Science | PLoS Medicine