Produção Nacional
Revisado por pares
Lorena S Derengowski, Calliandra Maria de-Souza-Silva, Shélida Vasconcelos Braz, Thiago Machado Mello-de-Sousa, Sônia Nair Báo, Cynthia Maria Kyaw, Ildinete Silva-Pereira,
Abstract Background Farnesol is a sesquiterpene alcohol produced by many organisms, and also found in several essential oils. Its role as a quorum sensing molecule and as a virulence factor of Candida albicans has been well described. Studies revealed that farnesol affect the growth of a number of bacteria and fungi, pointing to a potential role as an antimicrobial agent. Methods Growth assays of Paracoccidioides brasiliensis cells incubated in the presence of different concentrations of farnesol ...
Tópico(s): Plant-Microbe Interactions and Immunity
2009 - BioMed Central | Annals of Clinical Microbiology and Antimicrobials
Produção Nacional
Revisado por pares
Clináscia Rodrigues Rocha Araújo, Thiago de Mello Silva, Mônica Maria de Almeida Lopes, Paula Villela, Antônio Flávio de Carvalho Alcântara, Nísia Andrade Villela Dessimoni Pinto,
The in vitro antioxidant capacity, total phenolic content and mineral elements of the fruit peel of Myrciaria cauliflora were investigated. The antioxidant capacity was analyzed by the diphenylpicrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) and β-carotene methods. The assays based on the DPPH (EC50 = 3.18 g sample/g DPPH), ABTS•+ (1017 μmol Trolox/g sample), FRAP (1676 µM Fe2SO4/g sample) and β-carotene/linoleic acid (70% of oxidation ...
Tópico(s): Phytochemistry and Biological Activities
2013 - INSTITUTO DE TECNOLOGIA DE ALIMENTOS | Brazilian journal of food technology/Brazilian Journal of Food Technology
Produção Nacional
Revisado por pares
Ariane Leite Rozza, Thiago de Mello Moraes, Hélio Kushima, Domingos Sávio Nunes, Clélia Akiko Hiruma-Lima, Cláudia Helena Pellizzon,
This study aimed to evaluate the gastroprotective mechanism of action of the essential oil of Croton cajucara Benth. (Euphorbiaceae) stem bark in ethanol-induced gastric ulcers and its in vitro anti-Helicobacter pylori activity. The involvement of heat-shock protein-70, vasoactive intestinal peptide, glutathione, nitric oxide, and nonprotein sulfhydryl compounds in the gastroprotective effect was determined in male Wistar rats. The minimum inhibitory concentration against H. pylori was determined ...
Tópico(s): Insect Pest Control Strategies
2011 - Mary Ann Liebert, Inc. | Journal of Medicinal Food
Produção Nacional
Revisado por pares
Ariane Leite Rozza, Thiago de Mello Moraes, Hélio Kushima, Alexandre Tanimoto, Márcia Ortiz Mayo Marques, Taís Maria Bauab, Clélia Akiko Hiruma-Lima, Cláudia Helena Pellizzon,
Citrus lemon (CL) belongs to Rutaceae family and is popularly known in Brazil as limão siciliano. The phytochemical analysis of CL fruit bark essential oil showed two majority components, limonene (LIM) and β-pinene (PIN). This study aimed to evaluate the gastroprotective mechanism of action from CL, LIM and PIN in ethanol- and indomethacin-induced gastric ulcers and its in vitro anti-Helicobacter pylori activity. After ethanol-induced gastric ulcer, the ulcer area was measured and the stomachs were ...
Tópico(s): Piperaceae Chemical and Biological Studies
2010 - Elsevier BV | Chemico-Biological Interactions
Produção Nacional
Revisado por pares
Fábio Cruz Moleiro, Márcio Adriano Andréo, Raquel de Cássia dos Santos, Thiago de Mello Moraes, Clenilson Martins Rodrigues, Camila Bernardes de Andrade Carli, Flávia Cristine Mascia Lopes, Cláudia Helena Pellizzon, Iracilda Zeppone Carlos, Taís Maria Bauab, Wagner Vilegas, Clélia Akiko Hiruma-Lima,
Mouriri elliptica Martius (Melastomataceae) is species reputed in folk medicine to heal gastric ulcer and gastritis. Methanolic extract (ME) and ethyl acetate fraction (EAF) from leaves of Mouriri elliptica were evaluated for their gastroprotective, healing, immunological, toxicological and anti-Helicobacter pylori activities. The gastroprotective action of ME and EAF was evaluated in rodent experimental models and to elucidate mechanisms of action, the antisecretory action, involvements of NO, SH, ...
Tópico(s): Phytochemical Studies and Bioactivities
2009 - Elsevier BV | Journal of Ethnopharmacology
Produção Nacional
Revisado por pares
Thiago de Mello Moraes, Clenilson Martins Rodrigues, Hélio Kushima, Taís Maria Bauab, Wagner Villegas, Cláudia Helena Pellizzon, Alba Regina Monteiro Souza Brito, Clélia Akiko Hiruma-Lima,
Tópico(s): Food Science and Nutritional Studies
2008 - Elsevier BV | Journal of Ethnopharmacology
Produção Nacional
Revisado por pares
César Augusto Cuba Cuba, Vera Ferreira, Maria Luisa Furlin Bampi, Albino V. Magalhães, Philip D. Marsden, A. Vexenat, Milton Thiago de Mello,
Foureen marmosets (Callithrix penicillata) were inoculated intradermally with promastigotes and/or amastigotes of Leishmania (Viannia) brazilensis (L. (V) b.) strains MHOM/BR/83/LTB-300MHOM/BR/85/LTB-12 MHOM/BR/81/LTB-179 and MHOM/BR/82/LTB-250. The evolution of subsequent lesions was studied for 15 to 75 weeks post-inoculation (PI). All but of the L. (V) b. injected marmosets developed a cutaneous lesion at the point of inoculation after 3 to 9 weeks, characterized by the appearance of subcutaneous nodules ...
Tópico(s): Primate Behavior and Ecology
1990 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Christian Derntl, Fernando Guzmán‐Chávez, Thiago Machado Mello-de-Sousa, Hans‐Jürgen Busse, Arnold J. M. Driessen, Robert L. Mach, Astrid R. Mach‐Aigner,
Sorbicillinoids are a diverse group of yellow secondary metabolites that are produced by a range of not closely related ascomycetes, including Penicillium chrysogenum, Acremonium chrysogenum, and Trichoderma reesei. They share a similarity to the name-giving compound sorbicillin, a hexaketide. Previously, a conserved gene cluster containing two polyketide synthases has been identified as the source of sorbicillin, and a model for the biosynthesis of sorbicillin in P. chrysogenum has been proposed. ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2017 - Frontiers Media | Frontiers in Microbiology
Produção Nacional
Revisado por pares
Thiago Machado Mello-de-Sousa, Alice Rassinger, Marion E. Pucher, Lílian dos Santos Castro, Gabriela Félix Persinoti, Rafael Silva‐Rocha, Márcio José Poças-Fonseca, Robert L. Mach, Roberto Nascimento Silva, Astrid R. Mach‐Aigner,
Trichoderma reesei is used for industry-scale production of plant cell wall-degrading enzymes, in particular cellulases, but also xylanases. The expression of the encoding genes was so far primarily investigated on the level of transcriptional regulation by regulatory proteins. Otherwise, the impact of chromatin remodelling on gene expression received hardly any attention. In this study we aimed to learn if the chromatin status changes in context to the applied conditions (repressing/inducing), and ...
Tópico(s): Fungal and yeast genetics research
2015 - BioMed Central | BMC Genomics
Produção Nacional
Revisado por pares
Thiago Machado Mello-de-Sousa, Alice Rassinger, Christian Derntl, Márcio José Poças-Fonseca, Robert L. Mach, Astrid R. Mach‐Aigner,
The ascomycete Trichoderma reesei is used for the production of plant cell wall-degrading enzymes in industrial scale. The interplay of the transactivator Xyr1 and the repressor Cre1 mainly regulates the expression of these enzymes. During induc-ing conditions, such as in the presence of sophorose, the transcription of the two major cellulase-encoding genes, cbh1 and cbh2, is activated as well as the expression of xyr1. In the presence of D-glucose carbon catabolite repression mediated by Cre1 takes ...
Tópico(s): Fungal and yeast genetics research
2015 - Bentham Science Publishers | Current Genomics
Produção Nacional
Thiago Machado Mello-de-Sousa, Rita Gorsche, Alice Rassinger, Márcio José Poças-Fonseca, Robert L. Mach, Astrid R. Mach‐Aigner,
Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulases. Due to three rounds of undirected mutagenesis its genetic background differs from the wild-type QM6a in many ways, of which two are the lack of a 83 kb large sequence in scaffold 15 and the partial lack of the gene encoding the Carbon catabolite repressor 1 (CREI). However, it is still ...
Tópico(s): Plant nutrient uptake and metabolism
2014 - Springer Science+Business Media | Biotechnology for Biofuels
Produção Nacional
Revisado por pares
Thiago Machado Mello-de-Sousa, Ildinete Silva-Pereira, Márcio José Poças-Fonseca,
Tópico(s): Enzyme Catalysis and Immobilization
2010 - Elsevier BV | Enzyme and Microbial Technology
Produção Nacional
Revisado por pares
Luciana O. Pereira, Leandro Santos Bicalho, Mariana Lopes, Thiago Machado Mello De Sousa, Sônia Nair Báo, Maria de Fatima Menezes Almeida Santos, Márcio José Poças-Fonseca,
Background: Bracken fern ( Pteridium aquilinum ) has been consumed by humans and animals for centuries. However, its consumption is associated with a high incidence of cancer in the upper digestory tract of different species. Although the oral cavity is the first site of contact with ingested toxic substances, the interaction of bracken fern composites with oral cell lines has not yet been studied. Methods: In order to study the biological responses of oral cells exposed to bracken fern, we evaluated ...
Tópico(s): Phytochemistry and Biological Activities
2009 - Wiley | Journal of Oral Pathology and Medicine
Produção Nacional
Revisado por pares
Juliana Costa Khouri, Inês S. Resck, Márcio José Poças-Fonseca, Thiago Machado Mello De Sousa, Luciana O. Pereira, Adriano Barros Barata de Oliveira, César Koppe Grisólia,
The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic ...
Tópico(s): DNA Repair Mechanisms
2007 - Brazilian Society of Genetics | Genetics and Molecular Biology
Agnes Przylucka, Günseli Bayram Akçapınar, Klaus Bonazza, Thiago Machado Mello-de-Sousa, Astrid R. Mach‐Aigner, Victor Lobanov, Hinrich Grothe, Christian P. Kubicek, Erik Reimhult, Irina S. Druzhinina,
Hydrophobins (HFBs) are small surface-active proteins secreted by filamentous fungi. Being amphiphilic, they spontaneously form layers that convert surfaces from hydrophilic to hydrophobic and vice versa. We have compared properties of the class II HFB4 and HFB7 from Trichoderma virens as produced in Escherichia coli and Pichia pastoris. Since the production in E. coli required denaturation/renaturation steps because of inclusion bodies, this treatment was also applied to HFBs produced and secreted ...
Tópico(s): Tardigrade Biology and Ecology
2017 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Produção Nacional
Revisado por pares
Paula Fernandes Tavares Cezar de Mello, Thiago Gomes de Toledo-Pinto, Carolinne de Sales Marques, Lucia Elena Alvarado-Arnez, Cynthia Chester Cardoso, Luana Tatiana Albuquerque Guerreiro, Sérgio Luiz Gomes Antunes, Márcia Rodrigues Jardim, Claúdia Covas, Ximena Illaramendi, Ida Maria Foschiani Dias‐Baptista, Patrícia Sammarco Rosa, Sandra Maria Barbosa Durães, Antônio Guilherme Pacheco, Marcelo Ribeiro‐Alves, Euzenir Nunes Sarno, Milton Ozório Moraes,
Mycobacterium leprae infects macrophages and Schwann cells inducing a gene expression program to facilitate its replication and progression to disease. MicroRNAs (miRNAs) are key regulators of gene expression and could be involved during the infection. To address the genetic influence of miRNAs in leprosy, we enrolled 1,098 individuals and conducted a case-control analysis in order to study four miRNAs genes containing single nucleotide polymorphism (miRSNP). We tested miRSNP-125a (rs12975333 G>T), ...
Tópico(s): Infectious Diseases and Tuberculosis
2014 - Public Library of Science | PLoS neglected tropical diseases
Produção Nacional
Revisado por pares
Eliane Gonçalves Gomes, U. G. P. de Abreu, João Carlos Correia Baptista Soares de Mello, Thiago Bernardino de Carvalho, Sérgio de Zen,
The cow-calf beef production sector in Brazil has a wide variety of operating systems. This suggests the identification and the characterization of homogeneous regions of production, with consequent implementation of actions to achieve its sustainability. In this paper we attempted to measure the performance of 21 livestock modal production systems, in their cow-calf phase. We measured the performance of these systems, considering husbandry and production variables. The proposed approach is based ...
Tópico(s): Agricultural Economics and Policy
2012 - Sociedade Brasileira de Pesquisa Operacional | Pesquisa Operacional
Produção Nacional
Revisado por pares
Genésio Pacheco, Milton Thiago de Mello,
Tópico(s): Bacteriophages and microbial interactions
1950 - American Society for Microbiology | Journal of Bacteriology
Produção Nacional
Revisado por pares
Clélia Akiko Hiruma-Lima, Clenilson Martins Rodrigues, Hélio Kushima, Thiago Mello Moraes, Solange de Fátima Lólis, Shirley Barbosa Feitosa, Luciana Di Pietro Magri, Fernanda Rocha Soares, Maíra Cola, Fabio D.P. Andrade, Wagner Vilegas, Alba Regina Monteiro Souza Brito,
Tópico(s): Phytochemical Studies and Bioactivities
2008 - Elsevier BV | Journal of Ethnopharmacology
Thiago de Mello - Nascido em 1926, na cidade de Barreirinha,interior do Amazonas, Thiago de Mello é autor de algumas das mais expressivas obras da literatura amazonense e brasileira. Iniciou seus estudos em Manaus, mas depois transferiu-se para o rio de Janeiro, onde cursou até o quarto ano da Faculdade de Medicina. dividido entre a arte poética e a ciência médica, optou pela primeira. Publicou seu primeiro livro Silêncio e palavra, em 1951. Thiago foi um dos intelectuais perseguidos pela ditadura militar ...
Tópico(s): Psychology and Mental Health
2000 - School of Communications and Arts of the University of São Paulo | Comunicação & Educação
Produção Nacional
Revisado por pares
Eduardo Muchiutti Kispergher, Camila Agner D’Aquino, Luís C. da Costa, Thiago Carvalho de Mello, Regina Weinschutz, Álvaro Luiz Mathias,
This study aimed to evaluate the effect of variations in organic load (hydraulic retention times - HRTs: from 2 to 0.5 day) and in alkalinity (NaHCO3 from 4,000 to 1,000 mg.L−1) on methane production. Biomass of sewage sludge was inoculated and stabilized on 1" polypropylene rings. The rings were immersed in the liquid phase (8.41 L) of an upflow anaerobic filter reactor (12.22 L). A solution of 5 g of whole milk powder per liter was used to simulate effluent from the dairy industry. Process effectiveness ...
Tópico(s): Phosphorus and nutrient management
2017 - Sociedade Brasileira de Engenharia Agrícola | Engenharia Agrícola
Produção Nacional
Revisado por pares
Luana Bandeira de Mello Amaral, Thiago Brasileiro de Vasconcelos, Fabiane Elpídio de Sá, Andréa Soares Rocha da Silva, Raimunda Hermelinda Maia Macena,
Resumo: A violência baseada no gênero tem se constituído em um fenômeno social que influencia sobremaneira o modo de viver, adoecer e morrer das mulheres. Este estudo visa conhecer as diferenças antes e após a criação da Lei Maria da Penha (LMP) nas agressões sofridas pelas mulheres que foram atendidas na Unidade de Proteção Especial do Estado do Ceará. Trata-se de um estudo seccional, exploratório-descritivo e documental. A amostra foi composta por 197 prontuários das mulheres atendidas em uma Unidade ...
Tópico(s): Sexual Assault and Victimization Studies
2016 - UNIVERSIDADE FEDERAL DE SANTA CATARINA | Revista Estudos Feministas
Produção Nacional
Revisado por pares
Bianca Zeponi Fernandes de Mello, Thiago Cruvinel, Daniela Ríos, María Aparecida de Andrade Moreira Machado, Fabrício Pinelli Valarelli, Thaís Marchini de Oliveira,
Ectodermal dysplasia is a rare congenital disease that affects several structures of ectodermal origin. The most commonly related oral characteristics are hypodontia, malformed teeth and underdeveloped alveolar ridges. New alternative treatments are needed due to the failure of the conventional prosthesis retention. This case report outlines the oral rehabilitation treatment of a 9-year-old girl with ectodermal dysplasia. The treatment was performed with conventional prosthesis upon mini-implants. ...
Tópico(s): Cleft Lip and Palate Research
2015 - Fundação Odontológica de Ribeirão Preto | Brazilian Dental Journal
Produção Nacional
Revisado por pares
Ivan Gonzales Gargate, Thiago Castilho de Mello,
In this paper we prove that the image of multilinear polynomials evaluated on the algebra UTn(K) of n × n upper triangular matrices over an infinite field K equals Jr, a power of its Jacobson ideal J = J(UTn(K)). In particular, this shows that the analogue of the Lvov—Kaplansky conjecture for UTn(K) is true, solving a conjecture of Fagundes and de Mello. To prove that fact, we introduce the notion of commutator-degree of a polynomial and characterize the multilinear polynomials of commutator-degree ...
Tópico(s): Advanced Differential Equations and Dynamical Systems
2022 - Hebrew University of Jerusalem | Israel Journal of Mathematics
Pedro Fagundes, Thiago Castilho de Mello,
We describe the images of multilinear polynomials of degree up to four on the upper triangular matrix algebra.
Tópico(s): Advanced Optimization Algorithms Research
2019 - University of Zagreb | Operators and Matrices
Produção Nacional
Revisado por pares
Eveline Queiroz de Pinho Tavares, Marciano Régis Rubini, Thiago Machado Mello-de-Sousa, Gilvan Caetano Duarte, Fabrícia Paula de Faria, Edivaldo Ximenes Ferreira Filho, Cynthia Maria Kyaw, Ildinete Silva-Pereira, Márcio José Poças-Fonseca,
Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris , the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35 kDa protein which was purified through a two-step chromatography procedure. The highest enzyme activity was detected ...
Tópico(s): Enzyme Catalysis and Immobilization
2013 - Hindawi Publishing Corporation | Enzyme Research
Produção Nacional
Revisado por pares
Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Márcio José Poças-Fonseca, Marlene Teixeira De‐Souza, Diorge P. Souza, J. R. M. de Almeida, Camila Marinho-Silva, Nádia Skorupa Parachin, Alessandra da Silva Dantas, Thiago Machado Mello-de-Sousa, Lídia Maria Pepe de Moraes,
DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understanding the life cycle of an organism. We used computational tools for data mining of genes involved in these processes in the pathogenic fungus Paracoccidiodes brasiliensis. Data ...
Tópico(s): Plant Disease Resistance and Genetics
2005 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Milton Thiago de Mello, Niber Paz M. Silva,
Experiments for the investigation of dehydrogenase activity of washed cells of a strains of Br. abortus and another of Br. suis in presence of different single added substrates are reported. The activity was measured as the amount of formazan produced by the reduction of 2, 3, 5-triphenyltetrazolum chloride acting as a hydrogen ions acceptor, at pH 7.0. In a general manner the dehydrogenase activity of Br. suis was much more intense than that of Br. abortus (fig. 5). In the conditions of the experiments ...
Tópico(s): Escherichia coli research studies
1955 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Produção Nacional
Revisado por pares
Raquel L. Silva, Thiago de Roure Bandeira de Mello, João Paulo Barreto Sousa, Lorena Carneiro Albernaz, Natália Mendes Gomes Magalhães, Lais S. Morais, Larissa R. Francisco, Walter Leal Filho, Laila Salmen Espíndola,
Insecticides have been used not only to control pests in agricultural production, but also in integrated vector management strategies to reduce populations of mosquitoes, and, consequently, decrease transmission of pathogens they carry. The Aedes aegypti mosquito is a vector of dengue, Zika, chikungunya and yellow fever viruses, whereas the common mosquito Culex quinquefasciatus is a vector of human filariasis and the West Nile virus. Natural insecticides constitute effective ecofriendly alternatives ...
Tópico(s): Insect and Pesticide Research
2022 - Elsevier BV | Industrial Crops and Products
Produção Nacional
Revisado por pares
Rafaella Silveira, Thiago de Roure Bandeira de Mello, M. R. Sartori, Gabriel Sérgio Costa Alves, Fernando Fonseca, Carla Simone Vizzotto, Ricardo Henrique Krüger, Mercedes Bustamante,
Biological nitrogen fixation (BNF) represents the main input source of N in tropical savannas. BNF could be particularly important for Brazilian savannas (known as Cerrado) that show a highly conservative N cycle. We evaluated the effects of seasonal precipitation and nutrient additions on the nifH gene abundance in soils from a long-term fertilization experiment in a Cerrado's native area. The experiment consists of five treatments: (1) control, (2) liming, (3) nitrogen (N), (4) nitrogen + phosphorus (NP), ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
2021 - Cell Press | iScience