Marcela Vlková, Zita Chovancová, Jana Nechvátalová, Ashley N. Connelly, Marcus D. Davis, Peter Slanina, Lucie Travnickova, Marek Litzman, Tereza Grymová, Přemysl Souček, Tomáš Freiberger, Jiří Litzman, Zdeněk Hel,
Common variable immunodeficiency disorders (CVID) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The specific mechanisms mediating immune deficiency in CVID remain to be determined. Previous studies indicated that immune dysregulation in CVID patients is associated with chronic microbial translocation, systemic immune activation, and altered homeostasis ...
Tópico(s): Immune Cell Function and Interaction
2018 - American Association of Immunologists | The Journal of Immunology
Anna Svatikova, Naima Covassin, Kiran R. Somers, Krishen Somers, Filip Souček, Tomáš Kára, Jan Bukartyk,
a Calculated using the 2-sample t test.b Performed by asking participants to sustain an isometric handgrip of one-third of maximum voluntary handgrip contraction, using a dynamometer.c Participants completed serial mathematical tasks as fast as possible.d Performed last because of its sustained effects.Participants immersed 1 hand into ice water.
Tópico(s): Eating Disorders and Behaviors
2015 - American Medical Association | JAMA
Josef Matoušek, Josef Souček, Tomáš Slavík, Milan Tománek, J. Eugene Lee, Ronald T. Raines,
Onconase (ONC) and bovine seminal ribonuclease (BS-RNase) are homologs of bovine pancreatic ribonuclease (RNase A). Unlike RNase A, ONC and BS-RNase can evade the cytosolic ribonuclease inhibitor protein and are potent cytotoxins. Here, the endogenous cytotoxic activities of ONC and BS-RNase are compared in a wide variety of assays. Injections of ONC into one or both testes of mice and rats evokes a stronger aspermatogenic activity than does the injection of BS-RNase. Epididymides exposed to ONC lose ...
Tópico(s): Renal and related cancers
2003 - Elsevier BV | Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Jií Dostál, Petr Hamal, Libuše Pavlíčková, Milan Souček, Tomáš Ruml, Iva Pichová, Olga Hrušková‐Heidingsfeldová,
ABSTRACT The yeasts of the genus Candida are opportunistic pathogens associated with the rising incidence of life-threatening infections in immunocompromised individuals. Secretion of aspartic proteinases has been determined to be one of the virulence factors of the pathogenic Candida species. To analyze the extracellular proteolytic activities of a large number of Candida clinical isolates, we developed a screening system based on a solid medium containing hemoglobin as the sole nitrogen source. ...
Tópico(s): Peptidase Inhibition and Analysis
2003 - American Society for Microbiology | Journal of Clinical Microbiology
Josef Matoušek, Giovanni Gotte, P Poučková, Josef Souček, Tomáš Slavík, Francesca Vottariello, Massimo Libonati,
Dimers, trimers, and tetramers of bovine ribonuclease A, obtained by lyophilization of the enzyme from 40% acetic acid solutions, were purified and isolated by cation exchange chromatography. The two conformers constituting each aggregated species were assayed for their antitumor, aspermatogenic, or embryotoxic activities in comparison with monomeric RNase A and bovine seminal RNase, which is dimeric in nature. The antitumor action was tested in vitro on ML-2 (human myeloid leukemia) and HL-60 (human ...
Tópico(s): PARP inhibition in cancer therapy
2003 - Elsevier BV | Journal of Biological Chemistry
Lucie Grodecká, Pavla Hujová, Michal Kramárek, Tereza Kršjaková, Tatiana Kováčová, Katarína Vondrášková, Barbora Ravčuková, Kristýna Hrnčířová, Přemysl Souček, Tomáš Freiberger,
Both variants affecting splice sites and those in splicing regulatory elements (SREs) can impair pre-mRNA splicing, eventually leading to severe diseases. Despite the availability of many prediction tools, prognosis of splicing affection is not trivial, especially when SREs are involved. Here, we present data on 92 in silico-/55 minigene-analysed variants detected in genes responsible for the primary immunodeficiencies development (namely BTK, CD40LG, IL2RG, SERPING1, STAT3, and WAS). Of 20 splicing- ...
Tópico(s): RNA and protein synthesis mechanisms
2017 - Elsevier BV | Clinical Immunology
Minna Kotilainen, Richard Krumpolec, Daniel Franta, Pavel Souček, Tomáš Homola, David Cameron, P. Vuoristo,
The thermal stability of copper substrate material used in solar thermal collectors was investigated with and without atomic layer deposited (ALD) hafnium oxide barrier films at temperatures of 200–400 °C. HfO2 films were studied as barriers against thermal diffusion of copper substrate atoms. The ALD HfO2 thin films were deposited in a thermal process at 200 °C using Tetrakis(Dimethylamido)Hafnium(Hf(NMe2)4) and H2O precursors, with 200, 400, and 600 cycles. The Cu substrates with and without HfO2 ...
Tópico(s): Silicon and Solar Cell Technologies
2017 - Elsevier BV | Solar Energy Materials and Solar Cells
Jakub Lokoč, Patrik Veselý, František Mejzlík, Gregor Kovalčík, Tomáš Soućek, Luca Rossetto, Klaus Schoeffmann, Werner Bailer, Cathal Gurrin, Loris Sauter, Jaeyub Song, Stefanos Vrochidis, Jiaxin Wu, Björn Þór Jónsson,
Comprehensive and fair performance evaluation of information retrieval systems represents an essential task for the current information age. Whereas Cranfield-based evaluations with benchmark datasets support development of retrieval models, significant evaluation efforts are required also for user-oriented systems that try to boost performance with an interactive search approach. This article presents findings from the 9th Video Browser Showdown, a competition that focuses on a legitimate comparison ...
Tópico(s): Domain Adaptation and Few-Shot Learning
2021 - Association for Computing Machinery | ACM Transactions on Multimedia Computing Communications and Applications
Luca Rossetto, Ralph Gasser, Jakub Lokoč, Werner Bailer, Klaus Schoeffmann, Bernd Muenzer, Tomáš Soućek, Phuong Anh Nguyen, Paolo Bolettieri, Andreas Leibetseder, Stefanos Vrochidis,
Despite the fact that automatic content analysis has made remarkable progress over the last decade - mainly due to significant advances in machine learning - interactive video retrieval is still a very challenging problem, with an increasing relevance in practical applications. The Video Browser Showdown (VBS) is an annual evaluation competition that pushes the limits of interactive video retrieval with state-of-the-art tools, tasks, data, and evaluation metrics. In this paper, we analyse the results ...
Tópico(s): Video Analysis and Summarization
2020 - Institute of Electrical and Electronics Engineers | IEEE Transactions on Multimedia
Jakub Lokoč, Gregor Kovalčík, Tomáš Soućek,
The known-item and ad-hoc video search tasks still represent challenging problems for the video retrieval community. During last years, the Video Browser Showdown identified several promising approaches that can improve the effectiveness of interactive video retrieval tools focusing on the tasks. We present a major revision of the SIRET interactive video retrieval tool that follows these findings. The new version employs three different query initialization approaches and provides several result ...
Tópico(s): Multimodal Machine Learning Applications
2018 - Springer Science+Business Media | Lecture notes in computer science
Luca Rossetto, Ralph Gasser, Silvan Heller, Mahnaz Amiri Parian, Loris Sauter, Florian Spieß, Heiko Schuldt, Ladislav Peška, Tomáš Soućek, Miroslav Kratochvíl, František Mejzlík, Patrik Veselý, Jakub Lokoč,
In the research of video retrieval systems, comparative assessments during dedicated retrieval competitions provide priceless insights into the performance of individual systems. The scope and depth of such evaluations are unfortunately hard to improve, due to the limitations by the set-up costs, logistics, and organization complexity of large events. We show that this easily impairs the statistical significance of the collected results, and the reproducibility of the competition outcomes. In this ...
Tópico(s): Video Analysis and Summarization
2021 - IEEE Computer Society | IEEE Multimedia
Jakub Lokoč, František Mejzlík, Tomáš Soućek, Patrik Dokoupil, Ladislav Peška,
Interactive video search systems effectively combine text-image embedding approaches and smart user interfaces allowing various means of browsing in intermediate result sets. In this paper, we combine features from VIRET and SOMHunter systems into a novel approach for segment based interactive video retrieval. Based on our SOMHunter log analysis and VIRET tool performance in known-item search tasks, we focus on two specific features – a combination of context-aware ranking by text queries and Bayesian- ...
Tópico(s): Video Analysis and Summarization
2022 - Springer Science+Business Media | Lecture notes in computer science
Irina Levchuk, Tomáš Homola, Gaurav Singhal, Juan José Rueda-Márquez, Julius A. Vida, Pavel Souček, Tomáš Svoboda, Elena Villar-Navarro, Olga Levchuk, Petr Dzik, Anna Lähde, Javier Moreno‐Andrés,
In this study TiO2/polysiloxane(SiBi) thin films modified with different concentrations of graphene oxide (GO) were prepared by ink-jet printing on flexible polyethylene terephthalate (PET) substrates. Prepared coatings were characterized by means of scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), ultraviolet photoelectron spectroscopy (UPS), Raman and water contact angle measurements. During photocatalytic tests strong change of color ...
Tópico(s): TiO2 Photocatalysis and Solar Cells
2022 - Elsevier BV | Chemical Engineering Journal Advances
František Lehár, Nándor Szegedi, Jakub Hejč, Jiří Jež, Filip Souček, Tomáš Kulík, A. Siruckova, Zoltán Salló, Klaudia Vivien Nagy, Béla Merkely, László Gellér, Zdeněk Stárek,
Interventional cardiology procedures may expose patients and staff to considerable radiation doses. We aimed to assess whether exposure to ionizing radiation during catheter ablation of supraventricular tachycardia (SVT) can be completely avoided.In this prospective randomized study, patients with SVT (atrioventricular re-entrant tachycardia n = 94, typical atrial flutter n = 29) were randomly assigned in a 1:1 ratio to catheter ablation with conventional fluoroscopic guidance (CF group) or with the EnSite ...
Tópico(s): Cardiac electrophysiology and arrhythmias
2022 - Oxford University Press | EP Europace
Masoud Shekargoftar, J. Pospı́šil, Matouš Kratochvíl, Julius A. Vida, Pavel Souček, Tomáš Homola,
Deposition of the electron transport layer (ETL) is an important step in the manufacture of low‐cost, solution‐processed perovskite solar cells (PSCs). Thermal annealing processes account for a major part of the energy consumption involved in the fabrication of PSCs. The current paper presents a plasma‐based and highly convenient method (5 min, <60 °C) for the deposition of SnO 2 films (PT‐SnO 2 ) from a colloidal nanoparticle dispersion. A comparative evaluation of PT‐SnO 2 films with those created by ...
Tópico(s): ZnO doping and properties
2021 - Wiley | Energy Technology
Tereza Grymová, Lucie Grodecká, Přemysl Souček, Tomáš Freiberger,
Mutations in the C1 inhibitor (C1INH) encoding gene, SERPING1, are associated with hereditary angioedema (HAE) which manifests as recurrent submucosal and subcutaneous edema episodes. The major C1INH function is the complement system inhibition, preventing its spontaneous activation. The presented study is focused on SERPING1 exon 3, an alternative and extraordinarily long exon (499 bp). Endogenous expression analysis performed in the HepG2, human liver, and human peripheral blood cells revealed several ...
Tópico(s): Peptidase Inhibition and Analysis
2019 - Elsevier BV | Molecular Immunology
Jakub Lokoč, Gregor Kovalčík, Tomáš Soućek,
During the last three years, the most successful systems at the Video Browser Showdown employed effective retrieval models where raw video data are automatically preprocessed in advance to extract semantic or low-level features of selected frames or shots. This enables users to express their search intents in the form of keywords, sketch, query example, or their combination. In this paper, we present new extensions to our interactive video retrieval system VIRET that won Video Browser Showdown in ...
Tópico(s): Video Analysis and Summarization
2019 - Springer Science+Business Media | Lecture notes in computer science
Jiřı́ Škvor, Petra Lipovová, P Poučková, Josef Souček, Tomáš Slavík, Josef Matoušek,
The antiproliferative and antitumor effect of wheat leaf ribonuclease was tested in vitro on the human ML-2 cell line and in vivo on athymic nude mice bearing human melanoma tumors. The antiproliferative activity of this plant ribonuclease was negligible in comparison with bovine seminal ribonuclease. In the experiments in vivo, a significant decrease of the tumor size, however, was observed in the mice treated with wheat leaf ribonuclease (27 kDa) compared with the control RNase A and polyethylene ...
Tópico(s): Plant Genetic and Mutation Studies
2006 - Lippincott Williams & Wilkins | Anti-Cancer Drugs
Lenka Špinarová, Jiřı́ Toman, J Pospísilová, Miroslav Souček, Tomáš Kára, Miloš Štejfa,
Aim: Correlation of five humoral markers with laboratory, echocardiographic and right heart catheterization parameters in patients with chronic heart failure. Study population: 29 patients, heart failure NYHA II and III, ejection fraction below 40% with coronary artery disease or dilated cardiomyopathy. Methods: evaluation of thromboxane, prostaglandin F (PGF), tumor necrosis factor (TNF) alpha, endothelin-1 and big endothelin rest levels and their correlation with: (1) laboratory parameters: Sodium, ...
Tópico(s): Advanced Chemical Sensor Technologies
1998 - Elsevier BV | International Journal of Cardiology
Jiří Souček, Tomáš Šturc, Ján Mareček,
The cultivation of linseed is an alternative to the production of flax stem, which is globally on the decline. The alternative method to the traditional harvest using the flax puller is increasing use of combine harvester. By this method the harvest efficiency will be increased, which represents a significant step to an improvement of economy of cultivation. Depending on the variety, the value of specific energy consumption was determined from (0.43 MJ/kg) up to (0.63 MJ/kg) if we use flax puller ...
Tópico(s): Varied Academic Research Topics
2017 - Mendel University Press | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
Julius A. Vida, Pavol Gemeiner, Michaela Pavličková, Martina Mazalová, Pavel Souček, Dušan Plašienka, Tomáš Homola,
We present a rapid and low-temperature (70 °C) route for the preparation of crystalline TiO 2 on Ti 3 C 2 T x MXene surfaces using atmospheric pressure oxygen plasma. Reactive oxygen species from plasma break the MXene structure and form TiO 2 nanoparticles.
Tópico(s): 2D Materials and Applications
2022 - Royal Society of Chemistry | Nanoscale
Leona Vavro, Kamil Souček, Daniel Kytýř, Tomáš Fíla, Zbyněk Keršner, Martin Vavro,
The article deals with the use of computed X-ray radiography to visualize the development of the fracture process zone in the rock samples. For radiographic observations during the three-point bending loading, glauconitic sandstone from the Řeka quarry (sometimes also referred to as Godula sandstone) was used. The chevron-notched cylindrical specimens with the diameter of 29 mm and 120 mm nominal length were prepared from the sandstone blocks. These specimens were subjected to the Chevron Bend (CB) ...
Tópico(s): Drilling and Well Engineering
2017 - Elsevier BV | Procedia Engineering
Ivana Musilová, Ctirad Andrýs, Marcela Drahošová, Ondřej Souček, Martin Štěpán, Tomas Bestvina, Richard Špaček, Bo Jacobsson, Teresa Cobo, Marian Kacerovský,
To evaluate umbilical cord blood interleukin (IL)-6 concentrations and the occurrence of fetal inflammatory response syndrome (FIRS) with respect to microbial invasion of the amniotic cavity (MIAC) and/or intraamniotic inflammation (IAI) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM).One-hundred-eighty-eight women with singleton pregnancies complicated by PPROM between gestational ages of 24 + 0 and 36 + 6 weeks were included in the study. Blood samples were obtained by venipuncture ...
Tópico(s): Maternal and Perinatal Health Interventions
2016 - Informa | The Journal of Maternal-Fetal & Neonatal Medicine
Jindřich Špinar, Jiří Vítovec, Miroslav Souček, Ladislav Dušek, Tomáš Pavlík,
The CORD trials tested ramipril and losartan in patients with hypertension. CORD A randomised 4016 patients with blood pressure (BP) 3 months. The patients discontinued ACEI and switched to losartan. After 1 month the BP decreased to 7.7/4.7 mm Hg (p<0.001) and after 1 year to 13.8/8.7 mm Hg (p<0.001). CORD B compared ramipril and losartan in 3813 patients with hypertension who were not being treated with an ACEI or ARB. The patients were randomised to ramipril (n=1926) or losartan (n=1887). After 1 ...
Tópico(s): Sodium Intake and Health
2009 - Elsevier BV | International Journal of Cardiology
Ladislav Peška, Gregor Kovalčík, Tomáš Soućek, Vít Škrhák, Jakub Lokoč,
The W2VV++ model BoW variant integrated to VIRET and SOMHunter systems has proven its effectiveness in the previous Video Browser Showdown competition in 2020. As a next experimental interactive search prototype to benchmark, we consider a simple system relying on the more complex BERT variant of the W2VV++ model, accepting a rich text input. The input can be provided by keyboard or by speech processed by a third-party cloud service. The motivation for the more complex BERT variant is its good performance ...
Tópico(s): Video Analysis and Summarization
2021 - Springer Science+Business Media | Lecture notes in computer science
Lukáš Mach, Helena Bedáňová, Miroslav Souček, Michal Karpíšek, Tomáš Konečný, Petr Němec, Marek Orban,
Cardiac surgery and cardiopulmonary bypass (CPB) have been shown to stimulate a systemic inflammatory response which has been associated with adverse postoperative outcomes. Adipose tissue, both epicardial (EAT) and subcutaneous (SAT), is a known source of inflammatory cytokines, but its role in the pathophysiology of surgery- and CPB-induced systemic inflammatory response has not been fully elucidated. Therefore, we conducted a study to establish levels of selected cytokines in EAT and SAT prior to ...
Tópico(s): Cardiac and Coronary Surgery Techniques
2016 - SAGE Publishing | Perfusion
Ivana Musilová, Ctirad Andrýs, Marcela Drahošová, Ondřej Souček, Lenka Plíšková, Martin Štěpán, Tomas Bestvina, Jan Malý, Bo Jacobsson, Marian Kacerovský,
The aim of this study was to evaluate the amniotic fluid cathepsin-G concentrations in women with preterm prelabor rupture of membranes (PPROM) based on the presence of the microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI).A total of 154 women with singleton pregnancies complicated by PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid cathepsin-G concentrations were assessed by ELISA. MIAC was ...
Tópico(s): Pregnancy-related medical research
2016 - Informa | The Journal of Maternal-Fetal & Neonatal Medicine
Martin Vavro, Kamil Souček, Tomáš Daněk, Dalibor Matýsek, L. Georgiovská, Vendula Zajícová,
Slate is a traditional stone product that has long been used for roofing widely around the world. The presence of iron sulphides is one of the most significant factors affecting roofing slate resistance to weathering and, therefore, the quality of slates and their use in construction. Iron sulphide oxidation is the main process of roofing slate degradation. Qualitative and quantitative analyses to determine iron sulphides in roofing slates are usually conducted using transmitted and reflected light ...
Tópico(s): Geological Formations and Processes Exploration
2018 - Geological Society of London | Quarterly Journal of Engineering Geology and Hydrogeology
Silvie Kremserová, Tomáš Perečko, Karel Souček, Anna Klinke, Stephan Baldus, Jason P. Eiserich, Lukáš Kubala,
Systemic inflammation accompanying diseases such as sepsis affects primarily lungs and induces their failure. This remains the most common cause of sepsis induced mortality. While neutrophils play a key role in pulmonary failure, the mechanisms remain incompletely characterized. We report that myeloperoxidase (MPO), abundant enzyme in neutrophil granules, modulates the course of acute pulmonary inflammatory responses induced by intranasal application of lipopolysaccharide. MPO deficient mice had ...
Tópico(s): Respiratory Support and Mechanisms
2016 - Hindawi Publishing Corporation | Oxidative Medicine and Cellular Longevity
Tomáš Roubı́ček, Ondřej Souček, Roman Vodička,
An isothermal small-strain model based on the concept of generalized standard materials is devised, combining Maxwell-type rheology, damage, and perfect plasticity in the bulk. An interface analogue of the model is prescribed at the lithospheric faults, exploiting concepts of adhesive contacts with interfacial plasticity. The model covers simultaneously features such as rupturing of the fault zone accompanied with weakening/healing effects and also seismic waves emission and propagation connected ...
Tópico(s): Rock Mechanics and Modeling
2013 - Society for Industrial and Applied Mathematics | SIAM Journal on Applied Mathematics